Plant Breeding and Genetics Commons^™

Response Differences Of Two Maize Hybrid Varieties To The Application Of Bioagent-Ameliorant And Chicken Manure In Fertile Soil, Endang Kantikowati, Karya, Yudi Yusdian, Dian Murti Minangsih, Tien Turmuktini, Erna Yuliani, Fija Faujiah

Jurnal Kultivasi

To ensure adequate nutrition for the growth of maize (Zea mays L.) in sustainable agriculture, it is necessary to apply bio-agent soil conditioner and manure. This study aims to determine the effect of doses of bioagent-ameliorant and chicken manure on growth and yield components. The research was conducted in highland with an altitude 800 m above sea level. This study used an experimental method with two sets of experiments, namely experiments using one and two-cobs maize cultivars. The experiment used a randomized block design (RBD) with two treatment factors and three replications. The first treatment factor was the dose of …

Origin, Rise And Development Of American Upland Cotton And Their Status At Present. Second Edition, Ed. By F.M. Bourland, J. O. Ware

AAES Research Reports and Research Bulletins

American Upland cotton (Gossypium hirsutum) is a Dixie product. Although the stocks of the species were brought from elsewhere, new types (through series of adaptational changes) formed this distinctive group—the final characteristics of which are a product of the Cotton Belt of the United States. These biological processes were considerably aided by man and the steps of development possibly were about as follows: (a) natural selection took place in the earlier introductions, (b) seed were saved from the more choice stocks, (c) series of subsequent introductions were obtained from the better sources, (d) these were acclimatized and the superior ones …

Mycorrhizal Feedbacks Influence Global Forest Structure And Diversity, Camille S. Delavaux, Joseph A. Lamanna, Jonathan A. Myers, Richard P. Phillips, Salomón Aguilar, David Allen, Alfonso Alonso, Kristina J. Anderson-Teixeira, Matthew E. Baker, Jennifer L. Baltzer, Pulchérie Bissiengou, Mariana Bonfim, Norman A. Bourg, Warren Y. Brockelman, David F.R.P. Burslem, Li Wan Chang, Yang Chen, Jyh Min Chiang, Chengjin Chu, Keith Clay, Susan Cordell, Mary Cortese, Jan Den Ouden, Christopher Dick, Sisira Ediriweera, Erle C. Ellis, Anna Feistner, Amy L. Freestone, Thomas Giambelluca, Christian P. Giardina, Gregory S. Gilbert, Fangliang He, Jan Holík, Robert W. Howe, Walter Huaraca Huasca, Stephen P. Hubbell, Faith Inman, Patrick A. Jansen, Daniel J. Johnson, Kamil Kral, Sabrina E. Russo, Et Al.

Center for Plant Science Innovation: Faculty Publications

One mechanism proposed to explain high species diversity in tropical systems is strong negative conspecific density dependence (CDD), which reduces recruitment of juveniles in proximity to conspecific adult plants. Although evidence shows that plant-specific soil pathogens can drive negative CDD, trees also form key mutualisms with mycorrhizal fungi, which may counteract these effects. Across 43 large-scale forest plots worldwide, we tested whether ectomycorrhizal tree species exhibit weaker negative CDD than arbuscular mycorrhizal tree species. We further tested for conmycorrhizal density dependence (CMDD) to test for benefit from shared mutualists. We found that the strength of CDD varies systematically with mycorrhizal …

Enabling Breeding For Fruitlet Freeze Tolerance In Peach, Ufuk Caglar

All Theses

Climate change is affecting the production of temperate fruit crops, with cold temperatures emerging as a critical abiotic stressor that limits plant growth and performance. Freeze damage, particularly in spring, has resulted in significant economic losses in peach production in the southeastern United States. Research efforts in peach and other Prunus species have primarily focused on studying dormancy-related traits associated with bloom time, such as chill and heat requirement, with fruitlet freeze tolerance not equally represented. Breeding for climate resilience in peach requires a combination of these traits to allow for late bloom via targeted chill requirement and high heat …

Evaluation Of Vegetative Indices To Determine Canopy Ground Cover For Winter Survival And Hybrid Necrosis In Winter Wheat, Micheal Young

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The benefit of unmanned aircraft systems and image processing methods in agronomic research across numerous crops has been well documented as has the importance of wheat, Triticum aestivum L., on the global food supply. Hence there is great interest in digital solutions applied to aspects of wheat breeding. A major trait of importance to winter wheat breeders in higher latitudes is winter survival, which can result in poor yield and performance if lines do not survive extreme cold. Scoring winter survival is most commonly based on visual score of 0% to 100% with the higher percentage conveying higher winter survival …

Arkansas Soybean Research Studies 2022, Jeremy Ross

Arkansas Agricultural Experiment Station Research Series

Arkansas is the leading soybean-producing state in the mid-southern United States. Arkansas ranked 11th in soybean production in 2022 when compared to the other soybean-producing states in the U.S. The state represented 3.04% of the total U.S. soybean production and 3.64% of the total acres planted in soybean in 2022. The 2022 state soybean average yield was 52.0 bushels per acre, tying the previous state yield record of 52 bushels per acre set in 2021. The top five soybean-producing counties in 2022 were Mississippi, Crittenden, Phillips, Poinsett, and Arkansas (Table 1). These five counties accounted for over 35.7% of the …

A Genome Wide Association Study Of Heat Tolerance In Snap Bean (Phaseolus Vulgaris), Morgan Stone

All Theses

Bean production in the United States has decreased while the temperatures have been steadily increasing, reaching new highs each year. Heat stress is detrimental to common bean (also known as snap bean) production. Symptoms of heat stress include decrease in pollen viability, shriveling of pods, and pod abortion making them unmarketable. Pod production of 323 snap bean accessions from a large diversity panel was assessed in a randomized complete block design with field trials at two different times in the spring season. The results show a significant decrease in the number of pods produced per plant and weight of pods …

Integrated Techniques To Identify And Characterize Resistance To Fusarium Wilt Caused By Fusarium Oxysporum, F. Sp. Vasinfectum Race 4 In Two Domesticated Gossypium Species, Stephen Parris

All Dissertations

Fusarium wilt of cotton (Gossypium hirsutum and G. barbadense), caused by Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4), is the most threatening disease faced by today’s cotton breeders. During the past 20 years, over $7 million have been dedicated to efforts in breeding upland cotton for resistance to FOV4 while yielding little to no results. To counteract this, we conducted a study in Clint, TX, that utilized control plots and geostatistical methods to adjust phenotypic data for the heterogeneous distribution of FOV4 in the field, improving the accuracy of genetic resistance assessments. Concurrently, early-generation genetic mapping in …

Corn And Grain Sorghum Performance Tests 2023, J. F. Carlin, R. B. Mulloy, R. D. Bond, J. C. Mccoy

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

The 2023 corn performance tests contained 46 hybrids and were conducted at the Northeast Rice Research and Extension Center (NERREC) at Harrisburg, the Northeast Rice Research and Extension Center (NEREC) at Keiser, the Lon Mann Cotton Research Station (LMCRS) near Marianna, the Rohwer Research Station (RRS) near Rohwer, and the Rice Research and Extension Center (RREC) …

Evaluation Of Oxyfluorfen For Use In Roxy Rice, Casey Hunter Arnold

Graduate Theses and Dissertations

Mid-southern U.S. rice (Oryza sativa L.) producers are challenged every year to control problematic barnyardgrass [Echinochloa crus-galli (L.) Beauv.] and weedy rice (Oryza sativa L.) because they have evolved resistance to most labeled herbicides. Oxyfluorfen is a Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 14 herbicide that inhibits protoporphyrinogen oxidase within the plant. Currently, there are no HRAC/WSSA Group 14 herbicides labeled for barnyardgrass or weedy rice control in midsouthern rice production. Therefore, oxyfluorfen is being evaluated for barnyardgrass and weedy rice control. Plant breeders at the University of California – Davis were able to breed …

Advances And Opportunities In Unraveling Cold-Tolerance Mechanisms In The World's Primary Staple Food Crops, Sofora Jan, Sachin Rustgi, Rutwik Barmukh, Asif B. Shikari, Brenton Leske, Amanuel Bekuma, Darshan L. Sharma, Wujun Ma, Upendra Kumar, Uttam Kumar, Abhishek Bohra, Rajeev K. Varshney, Reyazul Rouf Mir

Grain and Other Field Crops Research Articles

Temperatures below or above optimal growth conditions are among the major stressors affecting productivity, end-use quality, and distribution of key staple crops including rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays L.). Among temperature stresses, cold stress induces cellular changes that cause oxidative stress and slowdown metabolism, limit growth, and ultimately reduce crop productivity. Perception of cold stress by plant cells leads to the activation of cold-responsive transcription factors and downstream genes, which ultimately impart cold tolerance. The response triggered in crops to cold stress includes gene expression/suppression, the accumulation of sugars upon chilling, and signaling molecules, among …

Janus , A Spliceosome-Associated Protein, Promotes Mirna Biogenesis In Arabidopsis, Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MicroRNAs ( miRNAs ) are important regulators of genes expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex ( MC ) . Here, we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 ( DCL1 ) and SERRATE ( SE ) and directly binds the stem- loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript ( pri-miRNAs ) with MC are reduced. …

Quizalofop Resistance In Weedy Rice (Oryza Sativa L.) Is Mainly Conferred By An Ile1781leu Mutation, Fidel Gonzalez-Torralva, Jason K. Norsworthy

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Weedy rice (Oryza sativa L.) is an economically important weed species in rice (Oryza sativa L.) cropping systems. Two weedy rice samples (acc7 and acc8) suspected to be resistant to quizalofop-ethyl (quizalofop) were collected in Arkansas. In this research, susceptibility to quizalofop and resistance mechanisms have been explored. Dose-response assays displayed a resistance index of 42- and 58-fold for the acc7 and acc8, respectively. Experiments with metabolism inhibitors demonstrated that NBD-Cl (4-chloro-7-nitrobenzofurazan) increased quizalofop efficacy slightly in acc8, whereas malathion did not improve effectiveness in resistant samples. Sequencing of the ACCase gene displayed an Ile1781Leu substitution in the …

Genomic Variations Explorer (Genvarx): A Toolset For Annotating Promoter And Cnv Regions Using Genotypic And Phenotypic Differences, Yen On Chan, Jana Biová, Anser Mahmood, Nicholas Dietz, Kristin Bilyeu, Mária Škrabišová, Trupti Joshi

Biomedical Sciences

The rapid growth of sequencing technology and its increasing popularity in biology-related research over the years has made whole genome re-sequencing (WGRS) data become widely available. A large amount of WGRS data can unlock the knowledge gap between genomics and phenomics through gaining an understanding of the genomic variations that can lead to phenotype changes. These genomic variations are usually comprised of allele and structural changes in DNA, and these changes can affect the regulatory mechanisms causing changes in gene expression and altering the phenotypes of organisms.Inthisresearchwork,wecreatedtheGenVarXtoolset,thatisbacked by transcription factor binding sequence data in promoter regions, the copy number variations …

Arkansas Wheat Performance Tests 2022-2023, J. F. Carlin, R. B. Mulloy, R. D. Bond

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Crop Variety Improvement Program.

The tests provide information to companies developing cultivars and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests were conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location and cultural practice information accompany each …

Qtls And Candidate Loci Associated With Drought Tolerance Traits Of Kaybonnet X Zhe733 Recombinant Inbred Lines Rice Population, Yheni Dwiningsih, Julie Thomas, Anuj Kumar, Chirag Gupta, Navdeep Gill, Charles Ruiz, Jawaher Alkahtani, Niranjan Baisakh, Andy Pereira

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Rice is the most important staple crop for the sustenance of the world’s population, and drought is a major factor limiting rice production. Quantitative trait locus (QTL) analysis of drought-resistance-related traits was conducted on a recombinant inbred line (RIL) population derived from the self-fed progeny of a cross between the drought-resistant tropical japonica U.S. adapted cultivar Kaybonnet and the drought-sensitive indica cultivar ZHE733. K/Z RIL population of 198 lines was screened in the field at Fayetteville (AR) for three consecutive years under controlled drought stress (DS) and well-watered (WW) treatment during the reproductive stage. The effects of DS were quantified …

Cercocarpus Ledifolius Var. Intricatus ‘Doubledown’, Asmita Paudel, Youping Sun, Larry A. Rupp

Plants, Soils, and Climate Faculty Publications

Bonsai (tray landscape, potted scenery, potted landscape, miniature trees, and rockery) is an artistic horticulture practice of developing aesthetically formed trees and landscapes in miniature with appropriately aesthetic containers. This has been practiced over a few thousand years in oriental cultures, including the ancient Chinese tradition of penzai or penjing, from which the art originated; the miniature living landscapes of Vietnamese hòn non bộ; and the Japanese variations of bonsai and “tray planting” (Gustafson 1995). To produce bonsai plants that share similar shapes and styles of mature, full-size trees, cultivation techniques are used, including leaf trimming, …

Overcoming Genetic Paucity Of Camelina Sativa: Possibilities For Interspecific Hybridization Conditioned By The Genus Evolution Pathway, Rostyslav Y. Blume, Ruslan Kalendar, Liang Guo, Edgar B. Cahoon, Yaroslav B. Blume

Center for Plant Science Innovation: Faculty Publications

Camelina or false flax (Camelina sativa) is an emerging oilseed crop and a feedstock for biofuel production. This species is believed to originate from Western Asian and Eastern European regions, where the center of diversity of the Camelina genus is located. Cultivated Camelina species arose via a series of polyploidization events, serving as bottlenecks narrowing genetic diversity of the species. The genetic paucity of C. sativa is foreseen as the most crucial limitation for successful breeding and improvement of this crop. A potential solution to this challenge could be gene introgression from Camelina wild species or from …

A Facile Agrobacterium‑Mediated Transformation Method For The Model Unicellular Green Algae Chlamydomonas Reinhardtii, Truyen N. Quach, Shirley J. Sato, Mark R. Behrens, Paul N. Black, Concetta C. Dirusso, Heriberto D. Cerutti, Tom Elmo Clemente

Center for Plant Science Innovation: Faculty Publications

A reliable and simple Agrobacterium-mediated transformation system for the unicellular green algae model organism Chlamydomonas reinhardtii has been developed. The protocol has been successfully employed with both neomycin phosphotransferase II (nptII) and the phleomycin resistance (bleI) genes coupled with the selective agents paromomycin and zeocin, respectively. A set of binary vectors were assembled that carry the selectable marker cassettes under control either of the Rbcs2 alone or fused to the HSP270A leader sequence, PsaD, or ß-tubulin2 promoters. The corresponding T-DNA elements also harbored a cassette with a codon-optimized version of yellow fluorescence protein (YFP) under …

Genetic Improvement Of Tocotrienol Content Enhances The Oxidative Stability Of Canola Oil, Min Deng, Hao Chen, Wei Zhang, Edgar B. Cahoon, Yongming Zhou, Chunyu Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Tocotrienols and tocopherols, which are synthesized in plastids of plant cells with similar functionalities, comprise vitamin E to serve as a potent lipid-soluble antioxidant in plants. The synthesis of tocopherols involves the condensation of homogentisic acid (HGA) and phytyl diphosphate (PDP) under the catalysis of homogentisate phytyltransferase (HPT). Tocotrienol synthesis is initiated by the condensation of HGA and geranylgeranyl diphosphate (GGDP) mediated by homogentisate geranylgeranyl transferase (HGGT). As one of the most important oil crops, canola seed is regarded as an ideal plant to efficiently improve the production of vitamin E tocochromanols through genetic engineering approaches. However, only …

An Improved Digestion And Analysis Procedure For Silicon In Plant Tissue, Noah James Langenfeld, Bruce Bugbee

Plants, Soils, and Climate Faculty Publications

Silicon (Si) in plant tissues reduces abiotic and biotic stress, but it is incorporated as silica (SiO₂), which is difficult to solubilize for analysis. We modified an oven-induced tissue-digestion and analysis method to improve Si solubilization and validated its accuracy by quantifying the mass-balance recovery of Si from the hydroponic solution and plant tissues of cucumber (Cucumis sativus). Leaf, stem, and root tissues were dried, finely-ground, and digested in 12.5 molar sodium hydroxide at 95°C for 4 hours. Solutions were then acidified with 6 molar hydrochloric acid to achieve a pH below 2 for measurement of …

Driscoll’S Water-Use Efficiency (Wue) Strawberry Breeding Trial, Aidan Inoue

Plant Sciences

California’s water supply is seeing stress and growers are increasingly under pressure to lower their water use. While reducing irrigation to strawberry plants can be used to raise sugars (°bx), research on the impact of deficit irrigation on plant growth, fruit yield and quality are not yet quantified. The purpose of this experiment is to observe the effects of a ~50% deficit irrigation treatment on tabletop strawberry plants where drainage measurements and plant absorption can be recorded. Results indicate that plant vigor and total marketable yield are predominantly treatment dependent whereas fruit characteristics, including culls and berry size are reliant …

Maize Leaf Angle Genetic Gain Is Slowing Down In The Last Decades, Elvis F. Elli, Jode Edwards, Jianming Yu, Slobodan Trifunovic, Douglas M. Eudy, Kevin R. Kosola, Patrick S. Schnable, Kendall R. Lamkey, Sotirios V. Archontoulis

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Quantifying historical changes in maize leaf angle and factors affecting it can enhance our understanding of canopy architecture and light capture, and hence crop productivity. Our objectives were to (1) quantify leaf angle genetic gain per canopy position in Bayer's legacy maize (Zea mays L.) hybrids; (2) dissect the contribution of breeding from plant density on historical changes in leaf angle; and (3) synthesize our findings with literature to determine leaf angle changes over a century of breeding. We measured leaf angle in 78 maize hybrids released between 1980 and 2020 across eight environments in the US Corn Belt. …

Summaries Of Arkansas Cotton Research 2022, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

The current economic environment continues to drive the need to produce record or near-record yields to be profitable. Price volatility in 2022 added another level of difficulty in the quest for being profitable. The cotton market saw significant movement after reaching a $1.5802 per pound high in May 2022, cotton prices corrected and more than halved in value at the October 2022 low, and then consolidated around the 80 cents per pound level in December 2022 (Cotton is Consolidating). Great uncertainties still exist for the upcoming season, most of which are outside of our control. These include, but are not …

Exome-Based New Allele-Specific Pcr Markers And Transferability For Sodicity Tolerance In Bread Wheat (Triticum Aestivum L.), Roopali Bhoite, Rosemary Smith, Urmil Bansal, Mirza Dowla, Harbans Bariana, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Targeted exome-based genotype by sequencing (t-GBS), a sequencing technology that tags SNPs and haplotypes in gene-rich regions was used in previous genome-wide association studies (GWAS) for sodicity tolerance in bread wheat. Thirty-nine novel SNPs including 18 haplotypes for yield and yield-components were identified. The present study aimed at developing SNP-derived markers by precisely locating new SNPs on ~180 bp allelic sequence of t-GBS, marker validation, and SNP functional characterization based on its exonic location. We identified unknown locations of significant SNPs/haplotypes by aligning allelic sequences on to IWGSC RefSeqv1.0 on respective chromosomes. Eighteen out of the target 39 SNP locations …

Induction Of Ploidy Level On Three Patchouli (Pogostemon Cablin Benth) Cultivars By Colchicine In Vitro, Suseno Amien, Nikkita Putri Darmawan, Daniya Fathya

Jurnal Kultivasi

Patchouli plants produce essential oils and are used as a raw material and fixative agent for perfumes. The most widely cultivated patchouli plant in Indonesia is Aceh patchouli. Sexual propagation in Aceh patchouli plants is impossible as it does not flower. Genetic diversity can be elevated through another method, such as polyploidy induction using colchicine. This research aimed to evaluate the state-of-the-art application of polyploidization techniques as a breeding tool for callus-based explants. A completely randomized design with a factorial pattern was used in this experiment consisting of two factors, patchouli cultivars (Sidikalang, Tapak Tuan, and Lhokseumawe) and colchicine concentration …

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Research in Biology

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

Testing Of Resistance To Downy Mildew On Several Sweet Corn Inbred Lines Of 7th Generation, Heri Kustanto

Jurnal Kultivasi

Sweet corn is one of the most well-known horticultural crop commodities and can adapt to various climates and environments. Efforts to control downy mildew disease continue to be carried out in collaboration with various parties in different ways, including genetic improvement of the varieties planted. The purpose of this study was to test the resistance of the 7th generation of sweet corn inbred lines to downy mildew and to find out the phenotypic characters that affect downy mildew. The new inbred lines obtained from the results of a long breeding process will be better tested for resistance to downy mildew …

Breeding Cotton For Determinate Growth Habit: Increasing Cotton Fiber Yield By Remobilization Of End-Of-Season Perennial Reserves, Salman Naveed

All Dissertations

Cotton (Gossypium spp.) is a major export commodity for the U.S. Despite advances in plant breeding and management practices, the cotton yield gains in the U.S. have stagnated. Therefore, to increase cotton fiber yield, we undertook a novel approach by altering the growth habit of cotton from perennial to annual. In this effort, eight genotypes showing strong expression alleles for five floral induction and meristem identity genes were selected from 44 Upland cotton mini-core collection genotypes and genetically crossed in various combinations to stack the strong expression alleles in a genotype. These efforts have allowed us to develop high-yielding, …

Identifying Genetic Sources Of Anthracnose Resistance In Global Sorghum Lines, Mary-Frances Behnke

All Theses

Anthracnose of sorghum (causal agent: Colletotrichum sublineola) is the most detrimental disease of sorghum (Sorghum bicolor) worldwide, significantly reducing grain yield. This study includes a literature review of the disease and a meta-analysis of genome-wide association studies (GWAS) of anthracnose-resistance in sorghum as means to elucidate genetic sources of the trait. The meta-analysis includes genotypic and phenotypic data from 5 unique studies and 1,071 sorghum lines. Original genotyping-by-sequencing data from were obtained primarily from National Center for Biotechnology Information’s Sequence Read Archive (NCBI-SRA) and prepared and processed using the Tassel 5 GBS v2. pipeline. Phenotypic data were …