Pharmacology, Toxicology and Environmental Health Commons^™

Behavior Of Gold Nanoparticles In Physiological Environment And The Role Of Agglomeration And Fractal Dimension, Rose Cooper

Browse all Theses and Dissertations

The Present study was designed to examine the role of agglomeration, density and the resulting fractal dimension of nanomaterials in cell culture media. Studies were completed on the kinetics and the process of agglomeration, as well as how to calculate fractal dimensions. The correlation of such complex agglomeration patterns of nanomaterials in culture media, their translocation into cells, and toxic effects were observed. Our results showed that smaller primary particles agglomerated at an accelerated rate when compared to the larger primary particles. They also demonstrated increased cellular uptake, but exhibited lower fractal dimensions. The larger primary particle agglomerates displayed obvious …

The Effect Of Silver Nanoparticles On Synaptic Responses In The Lateral Giant Escape Circuit Of The Crayfish, Samantha N. Adkins

Theses, Dissertations and Capstones

The lateral giant neuron in the crayfish is a neuron that controls the reflexive escape response; the context and experience-dependent tuning of this response is critical for survival. Serotonin modulates synaptic responses in this neuron, and social experiences (stress) changes the modulatory role of serotonin. I investigated the mechanisms of induced synaptic response changes and serotonergic modulatory changes after silver nanoparticle exposure to ask if 1) contaminant induced stress changes serotonergic modulation in a manner consistent with social stress and 2) shed light on potential neurotoxic effects of widely used, but poorly understood nanomaterials. Our data show that stress induced …

Acute Toxicity Assessment Of N,N-Diethyl-M-Toluamide (Deet) On The Photosynthetic Activity Of The Dinoflagellate Gymnodinium Instriatum, Eloy Martinez, Sylvia Velez, Marietta Mayo, Miguel Sastre

Faculty Research & Creative Activity

Despite the ubiquitous occurrence of N, N-diethyl-m-toluamide (DEET) in aquatic systems, assessments evaluating the toxicity of DEET on phytoplankton species are summed to a single study on a unicellular green alga. In particular, the toxicological effects of DEET in dinoflagellates are unknown. In this study, we employed the mixotrophic dinoflagellate Gymnodinium instriatum as a study system to evaluate acute effects of DEET on the oxygen flux of laboratory cultures. This study reports an inhibitory reaction model of DEET described by the equation y = 4.99 x 0.54, where y represents the percent inhibition of oxygen flux and x represents DEET …

Using A Novel Optogenetic Approach To Directly Assess 5-Ht1a Somatodendritic Autoreceptor Function In Response To Chronic Selective Serotonin Reuptake Inhibitor Treatment, Kelly Marie Mcgregor

Wayne State University Dissertations

Antidepressant drugs are widely used but their mechanism of action remains only partially understood. One leading hypothesis holds that a key effect of chronic treatment with a Selective Serotonin Reuptake Inhibitor (SSRI) is loss of somatodendritic 5-HT1A receptor-mediated autoinhibition in serotonergic neurons of the dorsal raphe nucleus (DRN). However, technical limitations have prevented direct testing of this hypothesis. In the current study we took advantage of optogenetic strategies to assess the effects of the classic SSRI fluoxetine on 5-HT1A receptor-mediated autoinhibition. We conducted these experiments in mice expressing the light-sensitive ion channel Channelrhodopsin (ChR) in 5-HT neurons to facilitate their …

A Systematic Review And Meta-Regression Analysis Of Lung Cancer Risk And Inorganic Arsenic In Drinking Water., Steven H. Lamm, Hamid Ferdosi, Elisabeth K. Dissen, Ji Li, Jaeil Ahn

Epidemiology Faculty Publications

High levels (> 200 µg/L) of inorganic arsenic in drinking water are known to be a cause of human lung cancer, but the evidence at lower levels is uncertain. We have sought the epidemiological studies that have examined the dose-response relationship between arsenic levels in drinking water and the risk of lung cancer over a range that includes both high and low levels of arsenic. Regression analysis, based on six studies identified from an electronic search, examined the relationship between the log of the relative risk and the log of the arsenic exposure over a range of 1-1000 µg/L. The …

An Exploratory Study Of Dog Park Visits As A Risk Factor For Exposure To Drug-Resistant Extra-Intestinal Pathogenic E. Coli (Expec)., Lubna N. Ahmed, Lance B. Price, Jay P. Graham

Environmental and Occupational Health Faculty Publications

Background

Extraintestinal pathogenic E. coli (ExPEC) are common causative agents of urinary tract infections in humans. Dogs have been found to harbor ExPEC. This study tested stool samples from dogs (n = 16), the shoes of dog park visitors (n = 16) and the shoes of controls (n = 16) for ExPEC. Phenotypic resistance of isolates was characterized.

Findings

ExPEC were present in one-third of the dog stool samples, 9% of the samples from the shoes of dog park visitors and 6% of control samples. Half of the ExPEC isolates were multi-drug resistant.

Discussion

The findings suggest that dogs may …

Cip10 Optimization For 4,4-Methylene Diphenyl Diisocyanate Aerosol Sampling And Field Comparison With Impinger Method, Silvia Puscasu, Simon Aubin, Yves Cloutier, Philippe Sarazin, Huu Van Tra, Sébastien Gagné

Études primaires

4,4-methylene diphenyl diisocyanate (MDI) aerosol exposure evaluation in spray foam insulation application is known as being a challenge because the spray foam application actually involves a fast-curing process. Available techniques are either not user-friendly or are inaccurate or not validated for this application. To address these issues, a new approach using a CIP10M was developed to appropriately collect MDI aerosol in spray foam insulation while being suitable for personal sampling. The CIP10M is a commercially available personal aerosol sampler that has been validated for the collection of microbial spores into a liquid medium. Tributylphosphate with 1-(2-methoxyphenyl)piperazine (MOPIP) was introduced into …

Comparison Between The Asset Ez4 Nco And Impinger Sampling Devices For Aerosol Sampling Of 4,4′-Methylene Diphenyl Diisocyanate In Spray Foam Application, Silvia Puscasu, Simon Aubin, Yves Cloutier, Philippe Sarazin, Huu Van Tra, Sébastien Gagné

Études primaires

4,4′-methylene diphenyl diisocyanate (MDI) aerosol exposure evaluation in spray foam insulation application is known to be a challenge. Current available techniques are either not user-friendly or are inaccurate or are not validated for this application. A new sampler has recently been developed to address the user-friendliness issues with other samplers: the ASSET EZ4-NCO, but the use of this sampler in spray foam insulation applications has not been demonstrated or validated. Because of this, the current work was undertaken to provide a comparison of the ASSET sampler with an impinger method, considered to be the best available method in the context …

Short Native Α-Helical Cationic Antimicrobial Peptides: Promising Alternative Antibiotics, Jiraphun Jittikoon

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Synthesis And Characterization Of Sodium Alginate Conjugate: Preparation And Evaluation Of Transdermal Patches Of Losartan Potassium Using Sodium Alginate Conjugate, Satheesha Babu Bk, Srinivasa Rao K

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Relationship Between Carbamazepine Concentrations In Serum And Saliva Of Thai Epileptic Patients, Piyaporn Kaewdoung, Chartchai Puripokai, Chartchai Puripokai, Mayuree H. Tantisira, Somsong Laanwprasert

The Thai Journal of Pharmaceutical Sciences

This study aims to examine and verify the correlation between serum and saliva carbamazepine concentrations in Thai epileptic patients. The subjects were patients aged 15-60 years old who were under treatment with carbamazepine. Carbamazepine concentrati...

Development And Validation Of A Stability-Indicating Ultra-Performance Liquid Chromatographic Method For The Determination Of Rifampicin In Bulk Drug And Capsules, N. Swamy, K. Basavaiah, P. Vamsikrishna, G. Krishnamurthy

The Thai Journal of Pharmaceutical Sciences

A rapid method based on ultra-performance liquid chromatography (UPLC) was developed and validated for the specific determination of anti-tuberculosis drug rifampicin (RIF) in bulk form and capsules. The UPLC determination was carried out on a reversed-p...

Synthesis Of Novel Fused Heterocyclic Pyrazolo-Pyridazine Derivatives As Antimicrobial Agents, M. Alam, M. S. Zaman, K. Arora, A. Ahmad, S. A. Khan, A. Husain

The Thai Journal of Pharmaceutical Sciences

A series of pyrazolo-pyridazine derivatives (4a-j) were synthesized and evaluated for their antimicrobial activities with an aim to obtain promising antimicrobial agents. In the first step, 6-substituted phenyl-2,3,4,5- tetrahydropyridazin-3-one (2a,b) w...

Application Of Proteoglycans From Fish Cartilage For The Acceleration Of Burn Wound Healing, Sitthiphon Bunman, Pornanong Aramwit, Noppadol Larbcharoensub, Pasarapa Towiwat

The Thai Journal of Pharmaceutical Sciences

Cartilage contains a variety of proteoglycans (PGs) that can accelerate wound healing. The aim of this study was to evaluate the efficacy of proteoglycans extracted from fish cartilage for the acceleration of burn wound healing. Second degree burn wounds...

Evaluation Of Anti-Anxiety Activity Of Melissa Parviflora (Benth.) In Rats, Kundan Singh Bora, Aruna Dubey

The Thai Journal of Pharmaceutical Sciences

Melissa parviflora Benth. (Family: Lamiaceae) has been traditionally used as a tranquillizer, relaxants, nervine and sleeping aids throughout the world. The plant is reported to relieve tension and stress reactions, and widely valued for its calming prop...

In Vitro Biological Activities Of Black Pepper Essential Oil And Its Major Components Relevant To The Prevention Of Alzheimer’S Disease, Pattamapan Lomarat, Kittisak Sripha, Phanida Phanthong, Worawan Kitphati, Krit Thirapanmethee, Nuntavan Bunyapraphatsara

The Thai Journal of Pharmaceutical Sciences

Black pepper oil and its major components were investigated for the biological activities related to the prevention of AD. The inhibition of acetylcholinesterase (AChE) and β-amyloid aggregation, anti-inflammatory and antioxidant activities were examine...

Phytofabrication Of Silver Nanoparticles Using Horse Gram (Dolichos Biflorus L.) Seed Extract And Assessment Of Its Bactericidal And Antioxidant Activities, Mahadevan Vijayalakshmi, Thangavel Rajagopal, Jeyachandran Vijayakumar, Ponnirul Ponmanickam

The Thai Journal of Pharmaceutical Sciences

The silver nanoparticles synthesized by phytofabrication method receive bigger attention due to their fascinating properties. This study deals with the green mediated synthesis of silver nanoparticles using seed extract of horse gram (Dolichos biflorus L...

Screening Of Patients Receiving Selegiline From Methamphetamine Abusers Using The Urinary Amphetamine/Methamphetamine Ratio, Nunthika Kaewpunya, Wichian Tungtananuwat, Akravudh Viriyavejakul, Patramon Yongpanich, Nantana Thong-Ra-Ar, Chanchai Hosanguan, Somsong Lawanprasert

The Thai Journal of Pharmaceutical Sciences

Methamphetamine (MA), the major metabolite of selegiline excreted in urine, can cause false positive interpretation of patient receiving selegiline as a MA user based on the routine non-chiral separation method. This study aims to compare the ratio of am...

Protein-Protein Docking And Molecular Dynamics Simulations Elucidated Binding Modes Of Fubi-P62 Uba Complex, Sumet Chongruchiroj, Panida Kongsawadworakul, Veena Nukoolkarn, Montree Jaturanpinyo, Wichit Nosoong-Noen, Jiraporn Chingunpitak, Jaturong Pratuangdejkul

The Thai Journal of Pharmaceutical Sciences

The cytosolic Fau protein, a precursor of antimicrobial peptide, composes of ubiquitin-like domain FUBI at Nterminus and ribosomal protein rpS30 at C-terminus. Fau has been important in killing of intracellular Mycobacterium tuberculosis infection throug...

In Silico Docking And Pharmacophoric Analysis Of 3-Indolyl Pyridine Derivatives Toward Cyclooxygenase-2, Sukumar Bepary, Ge Hyeong Lee, Hee Jong Lim, In Kwon Youn

The Thai Journal of Pharmaceutical Sciences

A series of 3-indolyl pyridine derivatives having anti-inflammatory activities were docked into the indomethacin binding pocket of cyclooxygenase-2 enzyme. In this in silico study, the possible orientations of the molecules in the binding site of the rec...

Integration Of Thai Traditional Medicine Into Physicians' Practice Part 1: Conditions Facilitating The Integration, Katekaew Jehso, Sanguan Lerkiatbundit, Wantanee Wiroonpanich

The Thai Journal of Pharmaceutical Sciences

The purpose of this study was to gain a greater understanding of the physicians’ experiences in the conditions facilitating physicians to integrate Thai traditional medicine (TTM) into their current practice. A qualitative grounded theory method was de...

Integration Of Thai Traditional Medicine Into Physicians' Practice Part 2: Raising Consciousness, The Process Of Integration From Physicians' Experiences, Katekaew Jehso, Sanguan Lerkiatbundit, Wantanee Wiroonpanich

The Thai Journal of Pharmaceutical Sciences

The main purpose of the study was to gain deeper insight on physicians’ experiences in the integration process of Thai traditional medicine (TTM) into their practice. A grounded theory method was used to gain an understanding of the core process from p...

Hptlc Method For Simultaneous Estimation Of Ramipril And Losartan Potassium In Pharmaceutical Dosage Form: Development And Validation Consideration, Darshana A. Parmar, Dinesh V. Thakkar, Rashmin B. Patel, Mrunali R. Patel

The Thai Journal of Pharmaceutical Sciences

This paper describes validated high-performance thin-layer chromatography (HPTLC) methods for simultaneous estimation of ramipril (RAM) and losartan potassium (LOS) in pure powder and formulation. The HPTLC separation was achieved on an aluminum-backed l...

Anti-Platelet Aggregation Activity And Chemical Analysis Of Raw And Wine Stir-Fried Chuanxiong Rhizoma (Kot-Hua-Bua), Uthai Sotanaphun, Rataya Luechapudiporn, Prinyaporn Pradmeeteekul, Jankana Burana-Osot, Panadda Phattanawasin, Noppamas Soonthornchareonnon, Yenchit Techadamrongsin

The Thai Journal of Pharmaceutical Sciences

Chuanxiong Rhizoma (CR) is the dried rhizome of Ligusticum chuanxiong Hort. It is known as Kot-HuaBua in Thai traditional medicine. Two types of CR, raw CR and wine stir-fried CR, were commercially available in traditional drugstores in Thailand. Raw CR ...

Development Thai Herbal Medicine Knowledge Base Using Ontology Technique, Wachana Tungkwampian, Anuchai Theerarungchaisri, Marut Buranarach

The Thai Journal of Pharmaceutical Sciences

This study proposes a method for developing a knowledge base of Thai herbal medicine by using ontology techniques and developing its application as a semantic search system. Since knowledge of Thai herbal medicine is often presented in flat knowledge and...

Economic Impact Assessment On Good Pharmacy Practice Regulation In Thai Community Pharmacy, Thatjuta Wuttipanich, Tanattha Kitisopee

The Thai Journal of Pharmaceutical Sciences

Good Pharmacy Practice (GPP) in community and hospital pharmacy setting is a crucial standard for pharmacy services. The Thai Food and Drug Administration (Thai-FDA) realized the benefit of GPP and tried to implement this concept as a regulation for ever...

Preference On Medication Therapy Management (Mtm) Service: Results From Discrete Choice Experiment, Sutthawan Chancheochai, Rungpetch Sakulbumrungsil, Surachat Ngorsurachet

The Thai Journal of Pharmaceutical Sciences

Objectives: To assess preference and estimate willingness to pay (WTP) for medication therapy management (MTM) service. Method: A discrete choice experiment (DCE) was conducted in general population. The five relevant MTM service attributes (service sett...

Development And Validation Of A Stability-Indicating Hplc Method For Determination Of Clorazepate Dipotassium And Its Main Impurities In Bulk Drug And Capsules, Jankana Burana-Osot, Chanokporn Sukonpan, Sooksri Ungboriboonpisal

The Thai Journal of Pharmaceutical Sciences

A simple isocratic stability-indicating HPLC method was developed and validated for the simultaneous determination of clorazepate dipotassium in the presence of its main impurities; nordiazepam and 2-amino-5- chlorobenzophenone, in bulk drug and capsul...

Cocrystallization Of Artemisinin And Amodiaquine Hydrochloride, Wiriyaporn Sirikun, Jittima Chatchawalsaisin, Narueporn Sutanthavibul

The Thai Journal of Pharmaceutical Sciences

Artemisinin (ART) and amodiaquinedihydrochloride dihydrate (AQD) was selected as model host and guest molecules in the study. Cocrystallization of ART and AQD at mole ratio of 1:1 by temperature change technique using ethanol, new structure was found, ...

Elderly Patient Adherence To Ocular Pharmaceuticals; Impact Of Educational And Physical Barriers, Lucky Wagner

All Graduate Theses, Dissertations, and Other Capstone Projects

The purpose of this research was to assess and evaluate elderly patient's attitudes and behavior regarding educational and physical barriers and the impact on adherence to ocular pharmaceuticals. A 19 question survey was developed and in order to collect data. The survey was administered in person at the Edina Senior Center and the Burnsville Senior Center and online via Zoomerang survey software regarding elderly patient attitudes on educational and physical barriers and their impact on ocular pharmaceutical adherence. Findings from this study indicate elderly patients treating an ocular condition with ocular pharmaceuticals expressed strong agreement with improving upon both educational …