Pharmacology, Toxicology and Environmental Health Commons^™

Environmental Factors And Human Health Interactions : Ultrafine Particles, Temperature Variability, And Proximity To Power Stations, Ian Ryan

Legacy Theses & Dissertations (2009 - 2024)

Background: Air pollution leads to more than four million premature deaths worldwide and nearly 200,000 in the US each year. While there is extensive research relating PM2.5 to hospital visits for various health outcomes, there is relatively little research on ultrafine particles (UFPs), a particularly small and dangerous air pollutant. Another important exposure is temperature due to its association with dehydration, heat stress, and cardiovascular-related hospital admissions. However, relatively little research has been conducted on diurnal temperature range (DTR), or the range of temperature within a single day, which is an important indicator of climate change. Importantly, the interaction between …

Megacity : A Reservoir Of Toxic Environmental Contaminants And Health Disease Burden, Omosehin Daniel Moyebi

Legacy Theses & Dissertations (2009 - 2024)

Ambient air pollution is a global environmental problem of concern with more than 95% of the world population living in areas with unhealthy air. World Health Organization (WHO) in 2019 stated that air pollution is a principal environmental risk to human health with about 7 million premature air pollution-related deaths annually. Several epidemiological studies have linked morbidity due to cardiovascular diseases (CVDs) to ambient air pollution exposure, especially in North America and Europe. However, the effect of air pollution in Karachi, Pakistan is understudied, which is the case for many urban centers in the developing countries. Karachi is a megacity …

Identification Of Translesion Synthesis Inhibitors That Target Rev7/Rev3 Protein-Protein Interactions, Seema Patel

Honors Scholar Theses

Translesion synthesis (TLS) is a cellular mechanism utilized by cancer cells to tolerate DNA damage caused by chemotherapeutics, like cisplatin, by replicating past unrepaired lesions. This increases the rate of mutations, which leads to the emergence of drug-resistant cancer cells. Preliminary studies have shown that disrupting the protein-protein interactions (PPI) in the TLS heteroprotein complex increases cells’ sensitivity to first-line genotoxic chemotherapy, illustrating how inhibiting TLS assembly and function can significantly increase cancer cell death. These results underscore the therapeutic potential of targeting TLS PPI. Our current work in this area is focusing on inhibitors capable of disrupting the Rev7/Rev3 …

The Roles Of Pon2 In Mitochondrial Physiology, Lung Tumor Cell Proliferation, And Lung Tumorigenesis., Aaron Whitt

Electronic Theses and Dissertations

Paraoxonase 2 (PON2) is an intracellular, multifunctional enzyme with near-ubiquitous tissue distribution. Within cells, PON2 is localized to mitochondria and endoplasmic reticulum (ER), where it mitigates the formation of reactive oxygen species (ROS). PON2’s chief enzymatic function is its lactonase activity, through which it catalyzes the hydrolysis of a bacterial quorum-sensing molecule, N-(3-oxododecanoyl)-l-homoserine lactone (C12), effectively disrupting bacterial intercellular communication and protecting against infection. C12 is produced by the opportunistic pathogen Pseudomonas aeruginosa and has been shown to disrupt various aspects of eukaryotic host cell physiology and evoke apoptotic cell death through the activity of PON2. Additionally, PON2 has garnered …

Particulate Hexavalent Chromium Inhibits Homologous Recombination Repair By Targeting Rad51 Paralogs In Human Lung Fibroblasts., Aggie R. Williams

Electronic Theses and Dissertations

Hexavalent Chromium [Cr(VI)] is a known human lung carcinogen and general health hazard. The mechanism of carcinogenesis remains poorly understood, but chromosome instability (CIN) is the major theory in its carcinogenic mechanism. Homologous recombination (HR) repair is a DNA repair pathway that prevents CIN by repairing DNA double-strand breaks. RAD51, a key mediator protein of HR repair, along with the RAD51 paralogs (RAD51B, C, D, XRCC2, and 3) are required for HR repair. During HR, RAD51 loads and forms a helical nucleoprotein filament structure to promote DNA strand exchange and stimulate pairing activity of DNA. Cr(VI) exposures have been shown …

Monarch Butterflies: Rearing Methodologies And The Impact Of Fungicide And Insecticide Exposures, Matthew Greiner

Department of Entomology: Dissertations, Theses, and Student Research

The North American migratory monarch butterfly, Danaus plexippus L., populations has experienced a ca. 90% decline over the past two decades. The decline is attributed to climate change, loss and degradation of overwintering habitat, and the loss of milkweed plants in the midwestern United States. The remaining milkweed stands often occur close to agricultural fields, and efforts to establish additional milkweed on the landscape focus on agricultural systems. However, milkweed plants near agricultural fields are likely exposed to pesticides that could adversely impact monarch caterpillars. Effective management of milkweed habitat supporting monarch caterpillars requires knowledge about the toxicological impacts of …

Unveiling The Opioid Crisis: The Impact Of Opioids On Body Systems And Alternative Treatment Options To Reduce The Nationwide Opioid Epidemic, Krishna S. Patel, Elizabeth A. Manheim Ph.D.

Kean Quest

No abstract provided.

Cannabinoids And Retinal Fibrotic Disorders., Lucy June Sloan

Electronic Theses and Dissertations

Retinal fibrosis is detrimental to vision. Retinal pigment epithelial (RPE) cells contribute to several retinal fibrotic diseases. Upon exposure to TGF-β, a key fibrotic cytokine, RPE cells trans-differentiate to myofibroblasts marked by the integration of α-SMA fibers into F-actin stress fibers, which confer strong contractility. Myofibroblasts produce and contract the collagen-rich fibrotic scar and disrupt retinal architecture. In this study, we investigated the in vitro effects of the putative endocannabinoid compound N-oleoyl dopamine (OLDA) on TGF-β2 induced porcine RPE cell contraction and α-SMA expression. Using an in vitro collagen matrix contraction assay, we found that OLDA inhibited TGF-β2 induced contraction …

Regaining Effort-Based Food Motivation: The Drug Methylphenidate Reverses The Depressive Effects Of Tetrabenazine In Female Rats, Deanna Pietrorazio

Honors Scholar Theses

Tetrabenazine (TBZ), a vesicular monoamine transporter type 2 (VMAT-2) inhibitor, depletes dopamine and induces motivational deficits and other depressive symptoms in humans. Methylphenidate (MPH) is a dopamine transport blocker that is used to enhance motivational function. Previous studies have shown that in male rats, TBZ induces a shift in effort-related choice such that a low-effort bias is induced. In male rats this occurs at a dose range of 0.75-1.0 mg/kg TBZ, and this effect is reversible with co-administration of MPH. Recent studies have shown that females need a higher dose of TBZ (2.0 mg/kg) to show the low-effort bias. The …

Removal Of Arsenic (Iii) And Arsenic (V) From Aqueous Solution Via Common Sunflower Helianthus Annuus By Phytoremediation, Irving A. Vazquez Hurtado

Theses and Dissertations

Phytoremediation provides a cost-effective, non-invasive technique to remove contaminants from the soil and groundwater. Sunflowers are fast growing and have a showed effective at removing various pollutants from soil, including lead and other heavy metals. Health issues related to arsenic are mainly attributed to exposure of arsenite, whereas arsenate is much less toxic. The common sunflower Helianthus annuus was the species used to remove both arsenic (III) and As(V) ions from hydroponics solutions. In the present study sunflower seeds were germinated for a week, placed under the sun for a day and subsequently placed in a nutrient solution to aid …

Blood Lead Levels In Minority Children: A Case Of Environmental Racism, Erick Rivera

Sociology & Criminal Justice Theses & Dissertations

Racial minorities in the United States have suffered from being disadvantaged. Among these disadvantages is environmental racism. This includes minority communities being ‘sacrifice zones’ for toxic waste and being exposed to lead poisoning. The purpose of this study is to examine differences in blood levels between white children and children of color. This research will follow a bivariate model for the first research question, “Do youth of color (under the age of 18) have higher BLLs than white children?” The bivariate model will look at the relationship between ethno-racial group and BLLs. Specifically, an analysis of variance (ANOVA) will be …

Behavioral Effects And Neurobiological Mechanisms Of 3-Aminobenzimide In A Rodent Model Of Chronic Psychological Stress, Liza Wills

Electronic Theses and Dissertations

Major depressive disorder (MDD) is a leading cause of disability worldwide, with a lifetime prevalence rate of approximately 20%. Inadequate pharmacological treatment methods for MDD are a significant debilitating factor. Patient estimates suggest that the treatment resistance rate for pharmacological interventions is over 30%. Postmortem analyses of human tissue of individuals diagnosed with MDD have shown an increase in Poly (ADP-ribose) polymerase 1 (PARP-1) mRNA gene expression in prefrontal cortical white matter when compared to psychiatrically normal brain tissue. In order to further investigate this issue, the present study used the social defeat stress/chronic unpredictable stress (SDS + CUS) rodent …

Effects Of 2,4-Di-Tert-Butylphenol On Osteogenic And Myogenic Differentiation Of Human Induced Pluripotent Stem Cells, Thanh-Bình Dương

All Theses

Synthetic phenolic antioxidants (SPAs) are a class of compounds used to increase the durability of polymers as well as to increase the shelf-life of food, cosmetics, and other personal care items. Of these SPAs is 2,4-di-tert-butylphenol (2,4-DBP). 2,4-DBP is primarily used in polyethylene crosspolymer pipes, which are now used as a replacement for the traditional copper pipes for household water distribution. However, 2,4-DBP has been found to leach from these pipes into the water, enabling a mode of exposure to humans. 2,4-DBP has been detected in human urine, serum, but more importantly, maternal serum, placenta, and cord blood. …

Floating Treatment Wetlands For Brackish Waters: Plant Selection And Nutrient Uptake Potential., Andrea Landaverde

All Theses

Brackish water bodies in coastal regions provide critical ecosystem services that support human and environmental health. Anthropogenic activities such as agricultural and industrial activities, construction, urban settlements, and tourism contribute to increased inputs of nitrogen (N) and phosphorus (P) in brackish coastal ecosystems. Excess nutrients can lead to impaired water quality and affect marine organisms. Floating treatment wetlands (FTWs) are a vegetated-base technology used to remove contaminants from water column, that has been mainly studied and applied in freshwater systems. Application of FTWs in brackish systems requires further investigation, as high salinity in brackish waters could result in toxicity to …

Operatic Mysticisms: Mountains, Deserts, Waterscapes, Andrew Demczuk

Electronic Theses and Dissertations

Operatic Mysticisms: Mountains, Deserts, Waterscapes examines the ways we encounter environments as readers/viewers of operas, literature, film, and sound recordings, and how each medium requires different detail-gathering techniques. Respective to the previously mentioned mediums, Sun & Sea (2017), Mount Analogue (1952), El Mar La Mar (2017), and Energy Field (2010) are analyzed by engaging with environmental media studies and invention. Reflecting the nature of each landscape—summits of mountains, aporias of deserts, and mysteries of waterscapes—an elemental approach is taken in investigating how these spaces may be noticed, internalized, recorded, and traversed by both the artist and viewer. …

Hyper Stable Variants Of Fgf-1-Fgf-2 Dimer, Madison Shields Mcclanahan

Chemistry & Biochemistry Undergraduate Honors Theses

Fibroblast Growth Factors (FGFs), including FGF-1 and FGF-2, are proteins that play a crucial role in cell proliferation, cell differentiation, cell migration, and tissue repair. FGF-1 and FGF-2 are useful in accelerating the healing process in the human body; however, these proteins are naturally thermally unstable, resulting in a relatively low half-life in vivo. 1,8 In efforts to improve the stability of this protein, FGF-1 and FGF-2 proteins are engineered by combining the amino acid sequences of the two proteins to form a heterodimer and obtain novel properties. These two FGF variants are chosen for their specific wound healing capabilities. …

Atrazine Levels In Rural Nebraska Counties And Parkinson's Disease, Muhammad Zahid

Capstone Experience: Master of Public Health

Parkinson's disease (PD) is a multifactorial disorder, connecting both genetic susceptibility and environmental risk factors. Several epidemiological and experimental studies support the evidence that pesticides exposure is positively associated with enhancing neurodegenerative diseases. The high amount of atrazine used in Nebraska and increased PD cases led us to evaluate the association between the long-lived water-soluble pesticide atrazine and PD in Nebraska counties. A person's exposure to atrazine may come from inhalation, dermal absorption, and ingestion. Three types of atrazine data covering 1992 to 2017 were used for this study: applied atrazine, atrazine in surface water, and atrazine in groundwater. In …

Differential Transcriptome Analysis Reveals That Cache Valley Pm2.5 Triggers The Unfolded Protein Response In Human Lung Cells, Morgan Eggleston

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Worldwide, exposure to air pollution is a serious human health threat. Particulate matter (PM) air pollution is a mixture of suspended solid and/or liquid particles and particle size is determined by its aerodynamic diameter. Fine, or “respirable” particles, typically from vehicle emissions, manufacturing, power generation, agriculture, as well as secondary photochemical reactions, are classified as ≤2.5μm in diameter (PM_2.5). Upon inhalation, PM_2.5 particles can reach the lower, more sensitive regions of the lung, enter the bloodstream, and be distributed to other areas in the body. Large-scale epidemiology studies have shown that PM_2.5 air pollution is associated …

Assessment Of Soil, Water Contamination And Land Cover Changes In The Urban And Suburban Watersheds Of Houston, Texas, Felica Rena Davis

Dissertations (2016-Present)

Intense urbanization and increased anthropogenic activity are two important factors contributing to the decline of soil and water quality in Houston watersheds, which poses a great environmental and human health risks. This study, therefore, was designed to: 1) determine nutrient and metal concentrations in soil and water samples along Carpenters Bayou (CaB), Cedar Bayou (CB), Sims Bayou (SB), Spring Creek Bayou (SC) and Vince Bayou (VB), 2) analyze land cover changes in watersheds using Landsat image analysis and 3) evaluate socio-economic characteristics and human health risks for each watershed. Soil and water samples were collected from three different locations with …

Amelioration Of Acrolein Effects On The Small Intestine Of Rats By Curcumin And Compound 20, Syntia Engiasa Kwende

Dissertations (2016-Present)

Acrolein is an unsaturated aldehyde which produce chemicals used as an intermediate reactive aldehyde in the chemical industry. The primary component of acrolein, is derived from the incomplete combustion of wood, plastic, fossil fuels, the main component of cigarette smoke, the burning of fats and overheating of oil. The purpose of this research is to investigate whether curcumin and compound 20 prevents the induction of inflammatory and oxidative response caused by acrolein in the small intestine. Curcumin is the yellow pigment, lipophilic polyphenol substance that gives turmeric the medicinal properties. It can also define as the primary bioactive substance in …

Early Detection Of Atypical Motor And Neurobehavior Of Infants At Risk Secondary To Opioid Exposure: A Prospective Study, Kara Boynewicz

Electronic Theses and Dissertations

Prenatal opioid exposure has been studied in relation to infants' medical outcomes. However, large gaps exist in the literature supporting early identification of atypical neurobehavior and motor development of infants with prenatal opioid exposure. The purpose of the study was to investigate whether prenatal opioid exposure has a negative influence on a newborn infant’s neurobehavior and motor development to aid in the early identification of potential delays. Using a prospective quasi experimental design, infants motor development using the Test of Infant Motor Performance (TIMP) and neurobehavior using the NICU Neonatal Network Scale (NNNS) was assessed on 58 infants in a …

Evaluation Of Biochemical And Pathological Parameters At Different Doses Of Cisplatin In Experimental Animal Model: Toxicological Study On An Antineoplastic Drug, Farhana Sultana, Muhammed Mohibul Islam, Mohammad Nurul Amin, Nusrat Jahan, Asma Kabir, Talha Bin Emran, Bibek Chandra Sutradhar, Sujan Banik

Makara Journal of Health Research

Background: This study aimed to assess the effect of cisplatin-induced toxicities on biochemical and pathological parameters such as body, liver, and kidney weights, blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), and blood cells (RBCs and WBCs) in white Swiss albino mice.

Methods: Cisplatin’s potential toxic effects on body, liver, and kidney weights were evaluated using standard laboratory methods. Blood biochemical levels such as BUN, creatinine, and ALT levels were determined by an auto-hemolyzer using commercial diagnostic kits. Blood cells (RBCs and WBCs) were counted under a microscope by a hemocytometer.

Results: Cisplatin’s potential toxic effects on …

Barriers To Public Health In Latino American Indigenous Communities, Wilzave Quiles Guzman

Honors Program Theses and Research Projects

This paper focuses on barriers to public health in Latino American Indigenous communities. Indigenous people throughout the world are at a public health disadvantage due to a lack of clearly defined practices within the decision-making processes to plan, evaluate, and control finances from governmental and non-governmental entities in charge of managing health services. I argue that political, social, and institutional changes are still needed to achieve health equity in Indigenous communities and that these changes should go beyond implementing a universal health care program. I must recognize improvements made by many Latino American countries like Brazil, which prepared a plan …

Powering A Sustainable Electric Vehicle Future, Harmony Ruth, Jordan Brickman

Undergraduate Research Symposium Posters

Electric vehicle sales account for roughly 20% of total sales world wide and are projected to be 80% by 2050. China's electric vehicle market holds a 13.3% market share. China exported 550,000 electric vehicles in 2021. China represents a 54% share of the world's coal consumption as of 2020. Why study China on this issue? China is a major player in the World Economy.

Diversity Of Bacteriophage In Burkholderia Species, Abigail Price

Honors Projects

Bacteriophages are viruses that infect and replicate within bacteria and offer the potential of a therapeutic alternative to chronic infections that do not respond to antibiotic-based therapies. B. vietnamiensis is one of a number of Burkholderia species involved with chronic drug resistant infections in the lungs of individuals with compromised respiratory systems, as found in those with chronic obstructive pulmonary disease and, most especially, are of particular significance in patients with cystic fibrosis. The diversity of the Burkholderia species is explored by using online databases and looking at bacteriophage or phage-encoding viruses found in B. vietnamiensis. The open reading frames …

Aeromonas Phage Research And The Public Health Impact Of Antibiotics In Aquaculture Workers, Madelyn Merchant

Honors Projects

One of the most common fish diseases in aquaculture is Aeromonas infection. The most common way to treat this infection is through antibiotics. The bacteria in the fish can become antibiotic-resistant and perpetuate the disease. The diseases in fish create a huge financial loss and the industry loses $6 billion per year due to diseases in fish. An alternative to antibiotics is bacteriophage which causes less environmental degradation and is better for human gut flora. In aquaculture there have been examples of aquaculture workers becoming sick from the water in aquaculture ponds as well as from people eating the fish. …

Accumulation Of Persistent Organic Pollutants In Marine Mammals: A Case Study On Cetaceans, Pinnipeds, And Sirenians, Alydia Moorhead

All HCAS Student Capstones, Theses, and Dissertations

Persistent organic pollutants (POPs) are lipophilic semi-volatile organic chemicals that present a range of challenges to marine biota, specifically marine mammals that often occupy a high trophic position in the food web. POPs have become a global problem since they have been shown to cause immunologic, teratogenic, carcinogenic, neurological, and reproductive complications in living organisms due to their resistance to biodegradation and their lipophilic nature. Marine mammals can accumulate these toxic substances through direct ingestion, trophic transfer, adsorption, and maternal offloading. They are susceptible to both bioaccumulation and biomagnification of POPs. Accumulation of POPs is affected by many variables, including …

The Various Nervous System Drugs Commonly Used For Treatment And The Disparities In Availability Of These Drugs In Minority Groups, Iman Haurami

Honors Scholars Collaborative Projects

The nervous system is a complex network in our body that is responsible for actions, reflexes, and even sensations. With the brain being part of the CNS, many common nervous system disorders are due to a defect in the central nervous system and the functions of this organ. These disorders include mental health problems, degenerative diseases, seizures, infections, and other possible genetic diseases. Many of these diseases are common in society and constantly researched. Even though these diseases are common, treatment accessibility and effects on people of minority groups are overlooked compared to the focus on majority groups. For drug …

A Psychopharmacology Review Of Brexpiprazole, Nicole A. Marion

Conspectus Borealis

Brexpiprazole is an antipsychotic drug often used to help treat schizophrenia and major depressive disorder. This review paper breaks down the drugs history including what it is used for, how it is used, and how it was developed. Pharmacokinetics including administration routes, steady states, and metabolism. This review also describes pharmacodynamics, focusing on drug affinity. It will also discuss pharmacological effects pulling evidence from clinical trials. Last this review will describe the toxicology and safety of brexpiprazole.

Exchange Protein Directly Activated By Camp (Epac1) Protects Human Endothelial Cells From Tumor Necrosis Factor-⍺ (Tnf-⍺) Induced Cell Death, Anh Luu, Abigail Carpenter, Rosetta Tolley

ONU Student Research Colloquium

Millions of people are affected by diseases involving inflammatory states such as sepsis. Sepsis is associated with elevated tumor necrosis factor alpha (TNF-⍺) which mediates inflammation. Excessive TNF-⍺ contributes to death of endothelial cells (EC) with subsequent disruption of vascular endothelial function. Exchange Factor Directly Activated by cAMP (EPAC1) is a cyclic-AMP (cAMP) sensor that regulates multiple EC functions. We hypothesized that EPAC1 activation affects the response of EC to TNF-a. Microvascular EC were pretreated for 45 minutes with 100 mM 8-pCPT-2′-O-Me-cAMP (8-CPT, specific EPAC1 activator) or 10 mM forskolin (adenylyl cyclase activator that increases cAMP), then exposed to 5 …