Food Biotechnology Commons^™

Research Progress Of Concentrated Fruit Juice And Its Fermented Wine, Yin Zi-Ying, Guan Jun-Feng, Liu Jin-Long

Food and Machinery

This paper expounded the characteristics of concentrated fruit juice, and the advantages, disadvantages and optimization of common concentrated technologies, summarized the research status of fermented fruit wine with concentrated fruit juice as raw material, and looked forward to the research direction of fermented fruit wine with concentrated fruit juice as raw material.

Research Progress On The Effect Of Lactic Acid Bacteria On The Flavor Of Fermented Vegetables, Hou Xiao-Yi, Wang Jian-Hui, Deng Na, Li Hui, Zhang Bo, Cai Yong-Jian

Food and Machinery

In the process of vegetable fermentation, the variety and quantity of microorganisms are complex and changeable. Lactic acid bacteria (LAB), as the main power in the process of vegetable fermentation, participate in the regulation of various metabolic pathways and affect the formation of flavor. In this review, the changes of major flavor metabolites during vegetable fermentation and their internal relationship with the community structure and abundance of LAB were summarized, then the potential mechanism of LAB fermentation to produce flavor substances was discussed. Moreover, the application of LAB in vegetable fermentation was prospected.

Development And Control Of Biofilms: Novel Strategies Using Natural Antimicrobials, Sheetal Jha, Sanjeev Anand

Dairy Science and Food Science Faculty Publications: 2022 to Present

Separation membranes have a wide application in the food industry, for instance, in the clarification/fractionation of milk, the concentration/separation of selected components, and wastewater treatment. They provide a large area for bacteria to attach and colonize. When a product comes into contact with a membrane, it initiates bacterial attachment/colonization and eventually forms biofilms. Several cleaning and sanitation protocols are currently utilized in the industry; however, the heavy fouling of the membrane over a prolonged duration affects the overall cleaning efficiency. In view of this, alternative approaches are being developed. Therefore, the objective of this review is to describe the novel …

Angiotensin I-Converting Enzyme Inhibitory And Antioxidative Activities Of The Bioactive Peptides In The Crude Salt-Soluble Protein Hydrolysates Of Pili Nut (Canarium Ovatum Engl.) Cv. Mayon Kernel, Ella Mae Gamba, Lawrence Yves Uy, Mary Ann Torio

The Philippine Agricultural Scientist

The in silico enzyme digestion and peptide function annotation of pili nut (Canarium ovatum Engl.) 11S globulin protein sequence revealed the presence of bioactive peptides with antihypertensive and antioxidative activities. Molecular docking simulations showed that the proline-histidine (PH) ligand can better inhibit angiotensin I-converting enzyme (ACE) activity than captopril. This result was verified through biochemical assays. Pili kernel protein was extracted with 0.5 M NaCl buffer using the modified Osborne fractionation method, which yielded 100 mg of protein per gram of defatted pili kernel determined via Bradford assay. SDS-PAGE suggested that the protein extract had a major legumin-like (11S) …

Upcycling Imperfect Broccoli And Carrot To Healthy Snacks Using An Innovative 3d Food Printing Approach, Taylor Clary

Food Science Undergraduate Honors Theses

Vegetables are healthy foods with nutritional benefits; however, nearly one-third of the world’s fruits and vegetables are lost each year. The losses have the same nutritional value as an acceptable vegetable but have odd shapes or blemishes that make them unsuitable to consumers. 3D technology has numerous capabilities in the automotive, healthcare, and, now, food industries. Within the food industry, 3D food printing can bring customization to product design and nutrition as well as other advancements, including decreasing food waste. In this study, imperfect vegetables (i.e., broccoli and carrot) were upcycled into freeze-dried powders to improve their shelf-life before they …

Optimizing Soil Nutrient Management To Improve Dry Edible Bean Yield And Protein Quality, Emily Jundt

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Soil nutrient management is of the utmost importance to ensure crop yields are maximized to feed the world’s growing population. Micro and macronutrient deficiencies can be detrimental to plant growth and cause a drastic reduction in yield. Optimization of nutrient management needs extensive research. Nutrients such as nitrogen increase crop yield, even in legumes capable of using a large amount of nitrogen (N) via biological N fixation. Iron (Fe) fertilizers are also crucial for legumes as they are susceptible to Fe deficiency induced chlorosis.

Dry edible beans (Phaseolus vulgaris) are leguminous plants and excellent dietary protein sources. Great …

Cloud Point Extraction Coupled With Isooctane Back Extraction For Determination Of Eight Pesticide Residues In Tea Beverages By Gas Chromatography Mass Spectrometry, Xie Jie, Zhou Run, Kong Ling-Can, Meng Yuan-Hua, Wang Yu

Food and Machinery

Objective: Rapid determination of organochlorine and pyrethroid pesticide residues in tea beverages. Methods: Selecting the non-ionic surfactant PEG-4000 as cloud point extraction, pesticides were extracted with isooctane by ultrasonic back extraction. The upper layer of isoctane solution was analyzed by Gas Chromatography/Mass. The isooctane solution was separated by HP-5 ms UI capillary column and analyzed qualitatively and quantitatively by GC/MS. Results: The eight pesticides showed a good linear relationship between 0.01～2.0 and 0.02～4.0 mg/L, respectively, with the correlation coefficients (R) of over 0.995. The detection limit and quantification limit of this method were 0.003~0.008 mg/L and 0.01~0.02 mg/L, respectively. The …

Research On Trajectory Planning Algorithm Of Artificial Meat Adipose Tissue 3d Printing, Chen Lian-Jie, Ning Meng, Chen Hui-Tao, Zhang Fan

Food and Machinery

Objective: Aiming at the problems of unstable 3D printing quality and low printing efficiency of adipose tissue part of artificial meat based on plant raw materials, studied and improve the intelligent 3D printing trajectory motion algorithm matching with food materials, to reduce the accumulation and corners in the printing path. Methods: A trajectory planning method adapted to the characteristics of artificial meat adipose tissue raw materials was proposed. The complex section layer of the sliced model was divided into several simple polygon sub-partition contours using Bayesian algorithm, and an improved nearest neighbor method was used to determine the optimal motion …

Determination Of The Residues Of 9 Kinds Of Antiviral Drugs In Animal-Derived Sports Nutrition By Hilic-Ms/Ms, Guo Wei

Food and Machinery

Objective: This study aimed to help with strengthening the monitoring of antiviral drug residues in animal-derived sports nutrition products. Methods: A method for determining the residues of 9 kinds of antiviral drugs in animal-derived sports nutrition was established, by using hydrophilic interaction chromatography tandem mass spectrometry. The samples were extracted by 1% acetic acid-acetonitrile and purified by PRiME HLB column. The mobile phase system of acetonitrile-10 mmol/L ammonium acetate solution (containing 0.1% formic acid) was separated by Sielc Obelisc R column in gradient elution mode to achieve the separation of nine target components. Results: The 9 kinds of antiviral drugs …

Research On Control System Of Carya Cathayensis Shell Breaking Automatic Production Line Based On Plc, Zhao Yan-Li, Zhao Qian, Li Zhi-Qiang

Food and Machinery

Objective: In order to solve the problems of low automation level and low efficiency of the control system in the current automated production line of hickory shell breaking. Methods: On the basis of the existing automatic production line of hickory shell breaking, a control system of hickory shell breaking automatic production line based on PLC control technology is proposed. With PLC as the core, the control of the whole hickory shell breaking automatic production line was completed, including shell breaking machine, air separator, shell breaking separator, color sorter and other equipment. The feasibility of the method was verified by testing …

Evaluation On Fruit Texture And Quality Of Different Pear Varieties During Cold Storage, Zhou Hui-Juan, Ye Zheng-Wen, Zeng Si-Yi, Wang Xiao-Qing, Luo Jun

Food and Machinery

Objective: To determine the key points of pear quality formation and deterioration of different Pyrus pyrifolia pears during cold storage. Methods: Zaoshengxinshui, Hujingli18, Hujingli67 were used as materials, the fruit of three varieties with the same ripeness were stored in cold storage at （1.0±0.5） ℃ and relative humidity of 85%~90% for 60 days, fruit texture, total soluble solids, titratable acid, sugar and acid contents and 10 sensor values were determined every 10 days. Results: Hujingli18 and Zaoshengxinshui were the typical type of fructose accumulation,Hujingli 67 was the type of sorbitol accumulation, and all three varieties were the type of malic …

Research Progress Of Subcritical Extraction Technology In The Field Of Nutrient Component Extraction From Aquatic Products, Wang Wen-Jie, Chen Xiu-Biao, Chen Yun-Yun, Zhou Xu-Xia, Ding Yu-Ting

Food and Machinery

The fundamental extraction principle, production process, and instrument of the subcritical fluid (including water) technology in terms of the aquatic products materials were introduced in this review, and the academic progress of SFE technology in extracting and separating nutritional components from the aquatic products within the recent 10 years were summarized, in terms of proteins/peptides, lipids and polysaccharides from aquatic products. The advantages of different processing factors for the extraction of specific components were summarized, and the future development direction of SFE equipment and technology in the deep processing of aquatic products was prospected.

Design And Experiment Of One Cylinder Driving Five Rows Longan Core-Removing Mechanism, Xie Hai-Jun, Zhang Lian-Bo, Zhu Ming-Chao, Li Peng-Sheng

Food and Machinery

Objective: This paper puts forward one cylinder driving five-row longan core-removing mechanism for the low production efficiency of the pneumatic longan core-removing mechanism. Methods: The mechanism adopts one cylinder driving five rows handspikes in the direction, which transport longan to core-removing hole, after the longan is cut under the triangular blade, and another cylinder drives five handspikes in the vertical direction in order to remove longan core. Five longan cores is removed one working process, so that the production efficiency is improved. Results: The result of experiments showed that the order of influencing on longan core-removing loss rate is blade …

Flow Field Simulation And Experiment Of Mussel High Pressure Water Jet Cleaning Nozzle, Xie Fei, Yuan Yue-Feng, Wang Jia-Sheng

Food and Machinery

Objective： It solves the problems of large water consumption and poor cleaning effect existing in the traditional cleaning method of mussels. Methods： The geometric models of Axial fan-nozzle with different structural parameters were established by Solidworks software. After setting the parameters in fluent software, the velocity and water content of the internal and external flow fields of the jet were numerically calculated. Results： The larger the spray angle of the nozzle was, the faster the decay velocity of the flow field of the jet was, and the lower the water content was. The larger the equivalent diameter of the nozzle …

Effects Of Different Drying Methods On Degradation Characteristics Of Anthocyanins And Antioxidant Capacity In Blueberries, Zhao Wei, Huang Jia-Qi

Food and Machinery

Objective: Reducing the degradation of anthocyanins in blueberry during drying. Methods: The far infrared drying was applied to the drying of blueberries, and compared with traditional hot air drying to explore the degradation characteristics of major anthocyanins and the changes of PPO enzyme activity and antioxidant capacity of blueberries under different drying methods. Results: High retention of two major anthocyanins （D3G and C3G） in blueberries under far-infrared drying, and the second-order mathematical model could better simulate their degradation process. Compared with hot-air drying, the rate constants （k） and entropy values （ΔS） of far-infrared dried blueberry anthocyanins were smaller, and the …

Comparison Of Fermentation Effects Of Six Kinds Of Probiotics On Rice Wine, Huang Zhi-Guo, Pu Ling-Ping, Ren Zhi-Qiang, Cheng Guo-Fu, Deng Jie

Food and Machinery

Objective: This study aimed to investigate the effects of 6 kinds of probiotics and their different adding ways on the production of rice wine. Methods: The probiotic rice wine was prepared by adding Lactobacillus rhamnosus BV-77, Lactobacillus fermentans TF-331, Lactobacillus saliva AP-32, Lactobacillus johnsoni MH-68, Lactobacillus acidophilus TYCA-06 and Lactobacillus casei CS-773. To compare the effects of saccharifying enzyme alone, saccharifying enzyme and yeast at the same time and adding methods （probiotic bran koji and probiotic powder） on alcohol content, reducing sugar, total acid and sensory quality of rice wine. Results: Yeast can promote the fermentation of probiotic rice wine …

Progress In The Application Of Nano-Antibody In The Detection Of Small Molecular Contaminants In Food, Jin Ping, Ding Hong-Liu, Jin Xiao-Hong, Shen Qi-Liang, Fan Chun-Yan

Food and Machinery

The structure and characteristics of nano-antibody and their applications in the detection of mycotoxins, pesticide and veterinary drug residues, small molecule toxic substances and other small molecule pollutants were introduced, and the development direction of nano-antibody in biological detection technology and diagnosis research was prospected.

Rapid Determination Of Sulfites By Microextraction-Ion Chromatography In Red Wine, Wang Zhi-Hong, Lu Fei, Ni Yong-Fu, Cao Peng, Zhang Hong-Wei

Food and Machinery

Objective: In view of the improvement of the national standard method of distillation pretreatment, combined with the advantages of ion chromatography, a rapid determination of sulfite by microextract-ion chromatography in red wine was established. Methods: Sulfur dioxide was released by microextraction device, which was absorbed by sodium hydroxide solution to form sulfite, and then oxidized to sulfate. The samples were then filtered by 0.45 μm filter and determined by ion chromatograph. Results: The concentration of sulfite ion in range of 1.0～50 mg/L showed a linear relationship with the peak area, with correlation coefficient of 0.999 9, and the detection limit …

Evaluation Of Uncertainty For The Determination Of Thiocyanate In Cheese By Inhibited Conductivity Ion Chromatography, Pan Can-Sheng, Zhang Zhi-Zhou, Xia Bing, Zhang Fei, Wu Ping, Wu Ling-Tao

Food and Machinery

Objective: To evaluate the uncertainty of the determination of thiocyanate in cheese by inhibition conductance ion chromatography. Methods: The content of thiocyanate in cheese was determined by inhibition conductance ion chromatography and a mathematical model was established. The source of uncertainty introduced in the process of the experiment was described, and the synthetic uncertainty was calculated for each uncertainty component. Results: The determination of thiocyanate was （10.2±0.56） mg/kg, k=2, p=95%. Conclusion: The uncertainty in the measurement process mainly comes from the concentration of thiocyanate in the test solution, followed by sample recovery and repeatability experiments.

Quality Change Of Prawn During Low-Frequency Electric Field Ice Temperature Storage, Yu Ming, Xu Li-Juan, Chen Hai-Qing, Liang Zuan-Hao, Chen Shan-Shan, Liang Feng-Xue, Ao Fei-Fei

Food and Machinery

Objective: This study aimed to explore the feasibility of low-frequency electric field assisted controlled freezing-point storage (CFPS) technology improving the quality of shrimp. Methods: Low-frequency electric field was applied on the basis of controlled freezing-point storage (LFEF+CFPS) to preserve prawns, and the contents of malondialdehyde and salt soluble protein, as well as the changes of Ca²⁺-ATPase activity, pH value, shear force, color difference and protein molecular weight of prawns were monitored. Results: Compared with the conventional CFPS, the LFEF+CFPS had no significant effect on the content of malondialdehyde, salt-soluble protein, pH, shear force and color of prawn meat. …

Preparation Of Antioxidant Hydrolysate Of Agaricus Blazei Murill, Chen Zhuo-Juan, Ke Xiu-Xian, Huang Xiao

Food and Machinery

Objective: This study aimed to investigate the technological conditions for the preparation of antioxidant hydrolysate of Agaricus blazei Murill and analyze the nutritional components of the hydrolysate. Methods: Papain, cellulase and pectinase were prepared in m_papain∶m_cellulase∶m_pectinase ratio of 3∶4∶4 to dissolve the fruiting bodies of A. blazei. Response surface test was used to optimize the enzymatic hydrolysis process of A. blazei. Results: The optimal conditions of compound enzyme hydrolysis were as follows: compound enzyme dosage 0.5%, pH 6.2, hydrolysis temperature 49 ℃, hydrolysis time 147 min. Under these conditions, when the concentration of A. blazei …

Combination Of Hs-Spme-Gc-O-Ms And E-Nose Revealed The Characteristic Aroma Differences Of Watermelon Seeds Under Various Thermal Processing Treatments, Zhang Ru-Ru, Yu Xiong-Wei, Ouyang Hui, Peng Wu, Xu Wei-Jian, Fu Qin-Li, Li Shu-Gang

Food and Machinery

Objective: This study aimed to explore the differences in the aroma of watermelon seeds under different thermal processes. Methods: The analysis technics such as Headspace-Solid Phase Micro Extraction-Gas Chromatography-Olfactory-Mass Spectrometry (HS-SPME-GC-O-MS) and Electronic Nose (E-nose) were applied. Results: Thermal processing changed the flavor of watermelon seeds from light fragrance to strong roasted flavor; A total of 18 categories including 115 volatile compounds were detected with the help of HS-SPME-GC-O-MS, among which pyrazines, alcohols and aldehydes accounted for 34.25%, 21.07% and 10.99% of the total volatile compounds, respectively; In addition, 63 volatile compounds were discovered in watermelon seeds after microwave treatment, …

Construction And Research Of Bioreactor Simulation Scale-Up Model, Hu Yi-Wen, Han Fei-Fei, Jin Kui-Qi, Sun Yang

Food and Machinery

Objective: Building a perfect bioreactor scale-up model provides convenience for enterprise production, and a guidance for reactor manufacturers. Methods: Through the test of 1.5, 5, 20, 200 L scale reactor, the correlation and parameters of the volume dissolved oxygen coefficient kLa and mixing time tm were optimized and explored, the simulation amplification model of bioreactor was established, and the Scaleuper amplification system was built by MATLAB. Results: Compared with the test results, the simulation deviation of kLa was within ±15%. By introducing the definition of critical speed into the correlation of tm, the overall simulation deviation of tm was reduced …

Preparation And Analytical Application Of Molecularly Imprinted Polymer Electrochemical Sensor Forganoderic Acid A, Huang Gui-Zhen, Wang Qing-Xiang, Chen Jin-Mei, Zhan Feng-Ping, Wei Lan, Zheng Wan-Rong

Food and Machinery

Objective: Construction of a simple and rapid method for the analysis of ganoderic acid A （GAA）. Methods: The molecular imprinted polymer （MIP） that can specifically recognize ganoderic acid A （GAA） were prepared by cyclic voltammetric deposition using GAA as template molecule and o-phenylenediamine as functional monomer. The structure, morphology and electrochemical behavior of MIP were characterized by infrared spectroscopy, scanning electron microscopy and electrochemical methods. Using [Fe（CN）₆]^3-/4- as the active indicator, the analytical performance of the sensor for GAA was investigated. Porous MIP for GAA can be prepared on the electrode surface by one-step electropolymerization. Results: The …

Determination Of Bisphenol A And Nonylphenol In Food Packaging Material By High Performance Liquid Chromatography-Tandem Mass Spectrometry, Tang Ji-Wang, Yuan Lie-Jiang, Xiao Yong, Wang Shu-Xia, Pan Zhao, Deng Hang

Food and Machinery

Objective： A method based on high-performance liquid chromatography-mass spectrometry was developed for the simultaneous determination of bisphenol A and nonylphenol in food packaging material. Methods: The extraction solvent and extraction method were investigated. Solid-phase extraction （SPE） conditions were optimized. The effects of solid phase extraction column, loading solvent, eluting solvent and elution solvent on the purification effect were systematically analyzed. The samples were ultrasonic extracted with dichloromethaneand cleaned up on a solid-phase extraction （SPE） cartridge equipped with packing material of silicone. The separation was performed on a Venusil MP C₁₈（2）（100 mm×2.1 mm,2.6 μm）by gradient elution with 0.1% ammonia …

Simultaneous Determination Of 24 Kinds Of Pesticide Residues In Coffee Peel By Solid Phase Extraction-Gas Chromatography-Mass Spectrometry, Liu Chao, Mei Li-Bao, Yin Hai-Fei, Li Shu-Zhen, Long Yu-Zhou

Food and Machinery

Objective: A solid phase extraction-gas chromatography/mass spectrometry （GC-MS） method was developed for the simultaneous determination of 24 pesticide residues in coffee peel. Methods: The sample was wetted with water, extracted with acetonitrile, the extract solution is concentrated and then purified with ProElut TPC solid phase extraction column, eluted with acetonitrile and toluene=3∶1, after concentration, the sample was determinated by GC-MS and the peak area was measured by internal standard method. Results: The 24 pesticides were separated well within 28 minutes. The residue of pesticide was in the range of 0.016～0.400 mg/kg, the linearity of the method was good, the correlation …

Quality And Safety Management Research Of Traditional Fermented Meat Products, Zhao Ya-Li, Zhang Xiang-Mei, Lu Han, Yang Bei, Wen Gang

Food and Machinery

The quality safety risks and quality management issues of traditional fermented meat products were analyzed. This paper put forward suggestions on increasing research and development efforts, accelerating the knowledge benefit transformation process, standardizing the standard system, refining supervision standards, standardizing workshop production, optimizing product markets, strengthening the awareness of social participation, and promoting social co-governance, in order to improve the overall quality and safety level of the traditional fermented meat products industry.

Research On Multi-Agent Collaborative Governance Of Food Safety In Catering Services Enabled By Blockchain Technology, Yu Hui-Li, Liu Deng-Pan

Food and Machinery

Objective: Solving the problem of poor information exchange among the governance subjects will help to improve the governance effect. Methods: By sorting out the typical food safety crisis events of catering services in China in 2021, and analyzing the relationship between blockchain technology and food safety collaborative governance of catering services. Results: Governance information fragmentation, information asymmetry and difficulty in information traceability were realistic dilemma of multi-agent collaborative governance. Blockchain technology and multi-agent collaborative governance had the coupling of information decentralization, security, transparency and traceability. Conclusion: Construct the multi-agent collaborative governance framework enabled by blockchain technology, promote the in-depth integration …

Optimization On Extraction Of Cherry Seed Oil By Aqueous Enzymatic Method And Analysis Of Its Physical-Chemical Properties, He Lin-Feng, Wang Jing, Li Li-Qin, Xiao Di, Li Shang-Ze, Hu Yu

Food and Machinery

Objective: To improve the availability of cherry seed, cherry seed oil was extracted by the aqueous enzymatic method. Methods: the ratio of mixed enzymes was optimized by mixture design on the basis of single factor experiment. The basic indexes of oil such as fatty acid composition and physical and chemical properties were detected. Results: the optimum extraction conditions were a 2.0% addition of mixed enzyme comprising cellulase, pectinase and acid protease （0.67∶0.10∶0.23）, liquid to material ratio of 10∶1 (mL/g), enzymolysis temperature of 45 ℃, pH of 4.0 and time of 4.0 h. The recovery of oil was 93.18% and the …

Study On Supercritical Co2 Extraction Process, Volatile Components And Antioxidant Activity Of Rose Oil From Dianhong Rose, Liu Jin-Yun, Chang Jian, Jiang Zhuo-Fang, Xu Chong-Jun, Cheng Wan, Wu Heng

Food and Machinery

Objective: Comprehensive utilization of Dianhong rose resources to improve the added value of products. Methods: The single-factor experiment and response surface experiment were used to optimize the extraction process of supercritical CO₂ with essential oil yield as the measurement index. The composition and relative content of different rose oils were analyzed by GC-MS. The components and relative contents of different rose oils were analyzed by GC-MS, and the antioxidant activities of different rose oils were evaluated. Results: The results showed that the optimal extraction parameters were as followed: particle size of 40 mesh, extraction pressure of 25.5 MPa, extraction …