Food Biotechnology Commons^™

Bioresource Management Via Least Cost Feed Formulation For Red Tilapia (Oreochromis Sp.): Application Of Linear Programming Technique, Boudjema Kamel, Redhouane Benfares, Affaf Kord, Toufik Milla, Belkacem Guenachi, Lynda Frai, Rachida Itcher, Hocine Kherzane

Journal of Bioresource Management

The method of linear programming (LP) was utilized to determine the relationship between the composition of nutrients of each ingredients, specifications for raw materials, cost and requirements for nutrients of the red tilapia (Oreochromis sp.), in order to define: (1) least cost ration formulation, (2) least cost diet, (3) minimum price of producing a particular diet after meeting a number of requirements. The resulting LP models were solved using the simplex algorithm. Therefore, our results show that, formulated feed had high protein content (31.92 %), Lipid (2.53), Moisture (9.10 %) and Ash (9.00 %). The utilization of diet …

The Nutritional And Biotechnological Potential Of Amaranth: A Versatile Plant For Health And Sustainability, Zeliha Selamoglu, Muhammad Yasir Naeem, Yaira Rakhmetova, Tulkinzhon Gaipov

Journal of Bioresource Management

The amaranth plant, originating from Central and South America, was a staple food for ancient civilizations like the Aztecs and Inca. Today, it is cultivated across tropical and subtropical regions, including Africa, Asia, and North America. Belonging to the Amaranthaceae family and comprising approximately 60 species, amaranth is valued for its adaptability to various soil and climatic conditions. This versatile crop is a nutrient-dense food source with a diverse chemical composition and numerous bioactive compounds, particularly in its seeds. Naturally gluten-free, amaranth is ideal for individuals with celiac disease or gluten sensitivity. It provides a complete profile of essential amino …

Study Of The Incorporation Of Date Stone Powder On Technological And Rheological Characteristics Of Common Wheat Flour, Djilali Bouhadi, Hamza Belkhodja, Zouaoui Benattouche, Abderrahmane Remil, Ahmed Hariri

Journal of Bioresource Management

The fortification of bakery products with bioactive and functional substances is the main focus of food sciences. These nutrients include date fruits (Phoenix dactylifera L.) and their byproducts. This work aims to illustrate the effects of using date components in bakery products and to determine how these effects affect the products' technological, rheological, and sensory qualities. To achieve this purpose, date powder was introduced in bread making procedure with different rates (1, 3, 5, 7 and 9 %). Then, the physicochemical, sensorial and rheological characteristics was determined. In terms of bread qualities, date powder improved the volume and form …

Observations On The Biodegration By Solid State Fermentation Of The Giant Swamp Taro (Cyrtosperma Merkusii) Biomass By Two Endolichenic Fungi Isolates, Maria Helena, Helen Joan Lawalata, Dino Rahardiyan, Livana Dethris Rawung, Emma Mauren Moko

Makara Journal of Science

Giant swamp taro (GST/Cyrtosperma merkusii), a local North Sulawesi coastal plant, is underutilized but has high po-tential for cultivation as a paludiculture plant on rewetted peatland restorations. GST has high starch content and is currently being explored for potential use. This study explored the potential of using the biomass pulp yielded as resi-dues of the starch extraction process. Endolichenic fungi (Trichoderma reesei and Aspergillus aculeatus) isolated from local lichens, were inoculated on the GST biomass in a solid state fermentation (SSF). Findings of this study indicate that both isolates were effective in degrading the lignocellulosic biomass of GST over an …

Effects Of Heat Moisture Treatment On Dietary Fiber Utilization By The Gut Microbiota, Yichen Bai

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The present study investigates the impact of heat moisture treatment (HMT) on the utilization of dietary fibers from red lentil hulls and wheat bran and its subsequent effect on gut microbiota and short-chain fatty acid production. HMT significantly decreased the water absorption index in red lentil hulls but increased it in wheat bran. For resistant starch (RS), HMT elevated its content in red lentil hulls but diminished it in wheat bran. In terms of dietary fiber content, HMT increased soluble dietary fiber in red lentil hulls. However, carbohydrate utilization and SCFA production by gut microbiota did not significantly change after …

Development Of Fortified Blended Food Using Great Northern Bean, Aemiro Tadesse Zula

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Undernutrition is a global health challenge affecting people across all stages of life. While everyone is susceptible, certain groups face a significant burden. These include those living in poverty, young children, adolescents, older people, and lactating and pregnant women. Food insecurity is another primary global concern affecting billions of individuals worldwide and is closely associated with undernutrition. Implementing interventions to address these two significant global burdens is critical, and international organizations are playing a crucial role through food aid programs using Fortified Blended Foods such as corn-soy blend (CSB), corn-soy blend plus (CSB+), and corn-soy blend plus plus (CSB++). CSB+ …

Phenotypic Correlation, Path Analysis, And Quantitative Trait-Based Selection Of Elite Wheat Genotypes Under Heat Stress Conditions In The Terai Region Of Nepal, Radhakrishna Bhandari, Binod Panthi, Shivalal Nyaupane, Sandesh Shrestha, Prabin Sharma, Rajesh Kumar Gupta, Sansar Sahani, Mukti Ram Poudel

Makara Journal of Science

Wheat is one of the most important cereal crops worldwide, but the production and productivity of wheat is affected by heat stress. A field experiment using an alpha lattice design with seven blocks was conducted on 35 elite wheat genotypes in the Terai region of Nepal to identify the most appropriate trait resulting in a high-yielding wheat genotype with high tolerance to heat stress. Correlation analysis revealed that booting-to-heading duration (BtoH), booting-to-anthesis duration (BtoA), plant height (Ph), spike length (SL), spike weight (SW), thousand grain weight (TGW), straw yield (SY), and total biomass yield (TY) had a significant positive correlation …

Development Of Pork Meatballs Supplemented With Commercial Soluble Dietary Fibres And Mimic In Vitro Simulated Gastrointestinal Digestion, Chonlada Srikamwang, Pirawan Chaiwan, Sarana Rose Sommano, Malaiporn Wongkaew

International Journal of Health and Allied Sciences

This study investigated the effects of supplementing tapioca starch with commercially soluble dietary powder in meatballs to develop a low-glycaemic index (GI) food for people with high blood sugar levels. This work evaluates the physicochemical characteristics, texture profile analysis (TPA), proximate composition, and digestibility of carbohydrate in meatballs supplemented with commercial soluble dietary powder using 3x3 factorial completely randomised design. The physicochemical characteristics findings indicate that the colour values of GI meatballs, including L*, a*, and b*, ranged from 63.60 to 68.49, 1.52 to 3.05, and 13.86 to 16.56, respectively. The colour difference values of meatballs containing dietary fibre in …

Development And Validation Of Aronia Melanocarpa Berry Recipes For Home Canning: Integrating Thermal Lethality Studies, Microbiological Safety, And Antioxidant Analysis, Juan Diego Villegas Posada

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Aronia melanocarpa, also known as chokeberry, is gaining popularity for its high antioxidant content and health benefits, including reducing oxidative stress and inhibiting cancer gene expression. However, its characteristic bitterness and astringency limit its broader appeal. Chokeberry's low pH places it in the acid foods category, making it ideal for safe home canning. An acidic environment combined with heat treatment inhibits harmful bacteria like Clostridium botulinum. Home canning preserves food by sealing it in airtight containers and heating it to destroy spoilage microorganisms.

Developing safe and palatable recipes is essential for making chokeberries suitable for home canning. A …

Development Of An Egg-Specific Mass Spectrometry Targeted Method For Processed Food Matrices, Liyun Zhang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Egg is a major food allergen in the United States, causing symptoms from mild reactions to severe anaphylaxis. An egg-free diet remains the only management approach for egg allergy. Therefore, individuals and families living with egg allergies rely heavily on allergen statements on packaged food products for informed food choices. Currently, no labeling regulations exist for unintentionally introduced allergens, posing risks to consumers with egg allergies. Therefore, it is critical to have an accurate and reliable detection method in allergen control and management to determine the concentration of total protein from allergenic food sources. Traditional enzyme-linked immunosorbent assays are routinely …

Microbial Transfer And Cross-Contamination In Milling Facilities And Pathogen Survival In Milled Products And Baking Mixes, Aryany Leticia Peña-Gomez

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Wheat-based products, traditionally considered low-risk due to their low moisture content, have been involved in numerous foodborne outbreaks in recent decades. Salmonella and Shiga toxin-producing Escherichia coli (STEC) present in these products have caused significant numbers of hospitalizations and massive product recalls. The microbiological quality of flour is highly dependent on the incoming wheat, which is vulnerable to contamination from field to milling. Once pathogens are present in flour, they can survive for extended periods. Implementing strategies to mitigate the risk of pathogen contamination throughout the wheat processing chain is critical.

This study evaluated the distribution of E. coli …

The Effect Of Fat Content On The Inactivation And Recovery Of Listeria Spp. In Ready-To-Eat Foods After High Pressure Processing, Yhuliana Kattalina Niño Fuerte

Department of Food Science and Technology: Dissertations, Theses, and Student Research

High-pressure processing (HPP) is recognized by the United States Department of Agriculture Food Safety and Inspection Service (USDA FSIS) as a post-lethality treatment to control Listeria monocytogenes in ready-to-eat (RTE) meat and poultry products. A scoping review of 603 journal articles, which ultimately was narrowed down to 16 articles, revealed that the efficacy of HPP varies with fat content. The studies demonstrated that L. monocytogenes inactivation by HPP tends to be lower in RTE meats with greater than 5% fat content, compared to products with lower than 5% fat. The wide range of HPP parameters used in these studies …

The Effects Of Moisture On Microorganisms, Quality, And Flavor Of Liuyang Douchi During The Pile Fermentation Period, Jiang Liwen, Zhang Haodong, Qin Yeyou, Hu Jialiang, Liu Yang

Food and Machinery

Objective: Pile fermentation is an important process for the formation of Liuyang Douchi flavor, the initial moisture of the douchi qu during pile fermentation could induce the temperature changes which might further affected the microbial community and flavor compounds formation. Methods: In this paper, metagenomic sequencing, combined with headspace solid-phase microextraction-gas chromatography-mass spectrometry （HS-SPME-GC-MS）, was used to investigate the effects of different moisture （low 35%, medium 42%, high 50%） of the douchi qu on the bacterial community changes and volatile composition of Liuyang Douchi during pile fermentation. Results: The results showed that bacteria in high moisture douchi qu proliferated faster …

Research On The Law Of Impurity Removal Of Soybean Concentrated Lecithin And Substances Affecting Its Transparency, Fang Suqiong, Zhu Dongqi, Chen Wenrong, Yang Jiguo

Food and Machinery

Objective: This study aimed to explore the impurity removal law of soybean lecithin concentrate, analyze and identify the substances that affect the transparency of soybean lecithin concentrate. Methods: Using food-grade soybean lecithin concentrate as raw material and light transmittance as the main evaluation index, the effects of ceramic membrane pore size, material-liquid ratio, solvent system （n-hexane, n-hexane-anhydrous ethanol） and solvent ratio on impurity removal of soybean lecithin concentrate were investigated. Moreover, the collected impurities and soybean concentrated lecithin before and after impurity removal were analyzed and identified by lipidomics. Results: In the system of n-hexane-anhydrous ethanol, …

Analysis Of Physical And Chemical Indicators And Volatile Composition Changes During Aging Of Fuyuxiangxing Crude Baijiu, Lu Mengsi, Yu Ji, Yu Bing, Liang Chaoyu, Yao Maojun

Food and Machinery

Objective: This study aimed to optimize the aging technology of Fuyuxiangxing crude Baijiu and improve its quality. Methods: Testing and analyzing the new wine and the Fuyuxiangxing crude Baijiu aged for 1, 3, 5, 8 and 10 years allowed for the analysis of the physicochemical indexes such as pH, conductivity, viscosity, total acid and total ester. Additionally, the primary volatile flavor components of the six samples were qualitatively assessed using GC and GC-MS, and 26 volatile flavor components of the samples were quantitatively assessed using the external standard method. Results: With the increase of aging time, the conductivity increased, the …

Preparation And Properties Of Rice Protein Hydrolysate-Xanthan Gum Nanocarriers, Yang Yuqi, Yan Peizhou, Cheng Yunhui, Lin Qinlu, Wang Jing, Jiao Ye

Food and Machinery

Objective: To enhance the water solubility and stability of hydrophobic active substances. Methods: A nano-delivery system of rice protein hydrolysate （RPH）-xanthan gum （XG）-curcumin （Cur） was developed, wherein curcumin served as the delivery object, and the encapsulation efficiency, stability, antioxidant activity, and formation mechanism of the nanoparticles were determined and analyzed. Results: The encapsulation efficiency of RPH-XG-Cur nanoparticles reached 78.40%, with a particle size of 74.78 nm, PDI of 0.36, and Zeta potential of -27.93 mV. Remarkably, the incorporation of xanthan gum significantly improved the encapsulation performance as well as pH and ionic strength stability of the nanoparticles. Fourier infrared and …

Enzymatic Hydrolysis Preparation And Activity Analysis Of Antioxidant Peptides Derived From Walnut Dregs, Ni Ce, Cao Tianhong, Chen Min, Yu Xun, Wu Hao, Wen Li

Food and Machinery

Objective: This study focused on the identification and activity analysis of antioxidant active peptides in walnut dregs protein hydrolysates. Methods: Protein was extracted from walnut meal by fractionation method, and five kinds of proteolytic enzymes, including trypsin, papain, neutral protease, alkaline protease and flavor protease, were used to hydrolyze the proteins. The antioxidant activity of trypsin hydrolysate （TH）, papain hydrolysate （PH）, dispase hydrolysate （DH）, alcalase hydrolysate （AH） and flavourzyme hydrolysate （FPH） were evaluated and compared. The in vitro antioxidant activity of different concentrations of AH was determined by the detection indexes of DPPH free radical scavenging rate, ABTS free radical …

Machine Learning Prediction Of Copper Ion Interference With Mercury Ion Fluorescence Signals In Food Heavy Metal Detection, Song Fangliang, Liang Ying, Dong Jie, Wang Xuejie, Qian Jie

Food and Machinery

Objective: To construct an artificial intelligence prediction model to predict the selectivity of fluorescent probes for Hg²⁺ in a complex food testing environment in the presence of Cu²⁺ interference. Methods: Fluorescent probe technology combined with seven advanced classical machine learning models was used to predict and analyze the selectivity of the probe for Hg²⁺ in the presence of Cu²⁺ interference, and to compare the prediction effect of each model and select the optimal model. Results: Efficient models with accuracies of 0.786 and 0.810 in the cross-validation and test sets were successfully established based on Molecular 2D …

Determination Of Seven Nicotinamide Compounds In Health Products, Wu Ziling, He Minheng, Huang Jiale, Cai Xiayi, Sun Wenwen

Food and Machinery

Objective： This study aimed to establish an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of 7 nicotinamide compounds in health food. Methods： The samples were dissolved in 10% methanol water, extracted by ultrasonic, and 10 mmol/L ammonium acetate-acetonitrile was used as the mobile phase of gradient elution. The seven nicotinamide compounds were monitored by ESI, positive and negative ion scanning mode, and multiple reaction monitoring (MRM) mode. Results： The 7 nicotinamide compounds showed good linearity in the mass concentration range, and the correlation coefficients were greater than 0.996. The limit of detection (LOD) was 0.075~0.600 mg/kg, the …

Agile Innovation And Application Of Food Safety Supervision Mode, Zhang Yi

Food and Machinery

As innovative thinking, the concept of agile governance focuses on rapid response, efficiency, and human nature. Applying agile governance to food safety supervision will help to optimize the food safety supervision model and improve its efficiency. Based on agile governance thinking, the innovative application of agile thinking was proposed from three aspects: regulatory objectives, regulatory tools and regulatory subjects.

Research And Development Of Multifunctional Cooking Test Platform Combining Negative Pressure And Superheated Steam, Zeng Mingjian, Zhao Yadong, Xu Fengying, Liu Tianyi, Zhang Peiyao, Jiang Zhuo

Food and Machinery

Objective: To design a multifunctional cooking experimental platform with negative pressure and superheated steam, providing consumers with greener, healthier, and more diverse cooking methods. Methods: The research status of integrated cooking equipment was analyzed, and the application status of superheated steam and negative pressure technology in the food industry was summarized and analyzed. The performance parameters of the multifunctional cooking experimental platform were determined. Based on the characteristics of each function of the experimental platform, the cooking chamber, furnace door and other components were designed, and the overall plan of the experimental platform was developed. Results: The multi-functional cooking experimental …

Grading Crayfish By Estimating The Proportion Of Crayfish Head And Pincers Based On Deeplab V3+, Wang Zihao, Hu Zhigang, Fu Dandan, Jiang Yajun

Food and Machinery

Objective: To achieve reasonable and effective grading of live crayfish, and improve the work of grading crayfish. Methods: The construction of crayfish image shooting platform, to obtain the original image of crayfish, and the semantic segmentation dataset which segmented the three parts of the crayfish head, crayfish pincers, and crayfish tail was created. The correlation between the actual weight of three parts and the corresponding pixel size in the dataset was analyzed, and a new grading standard for crayfish which was according to the proportion of head and pincers in the whole crayfish was summarized. The DeepLab V3+ neural network …

Optimization Design And Experimental Research Of Sandwich Seaweed Tunnel Baking Equipment, Yu Chao, Chen Shufa, Niu Shuoshuo, Liu Yang, Qi Hengyang, Zhan Zhaoya

Food and Machinery

Objective: In order to solve the practical problem of uneven temperature distribution in the tunnel and easy adhesion of sandwich seaweed to the conveyor belt. Methods: The structure of tunnel baking equipment for sandwich seaweed and the software and hardware of fuzzy PID temperature control system were designed in this study, the baking device model was established based on the boundary conditions set in the actual production environment, after that, the CFD simulation optimization experiments was performed on the temperature field inside the tunnel. Results: The simulation experiment showed that the parameter combination of evenly arranged heating tubes, 30 heating …

Rapid Prediction Of Yellow Peach Spoilage Time Based On Portable Near Infrared Spectrometer, Zhou Xu, Yang Qianqian, Zhang Jin, Li Boyan

Food and Machinery

Objective: Portable near-infrared (NIR) spectroscopy and stoichiometric methods were used to predict the spoilage time of yellow peach. Methods: Diffuse reflectance spectra of yellow peach samples were collected using a portable NIR spectrometer. Spectral preprocessing techniques were employed to enhance data features, and partial least squares (PLS) regression was applied to establish a prediction model upon the spoilage time and NIR data of yellow peach. The model performance was evaluated by both root mean square error (RMSE) and coefficient of determination (R²). Results: An R² value of 0.63 and RMSE of 4.09 days were achieved for …

Non Destructive Detection Of Kiwifruit Sugar Content Based On Improved Woa-Lssvm And Hyperspectral Analysis, Zhang Kai, Zhu Lifang, Li Rulin, Wang Ziyi

Food and Machinery

Objective: Addressing the issues of poor accuracy and low efficiency in non-destructive testing methods for kiwifruit sugar content. Methods: Proposing a non-destructive testing method for kiwifruit sugar content that combined hyperspectral detection technology, least squares support vector machine, and improved whale algorithm. By collecting hyperspectral information of kiwifruit through a hyperspectral detection system, after preprocessing and feature wavelength screening, and then input into an improved whale algorithm optimized least squares support vector machine model to achieve rapid and non-destructive detection of kiwifruit sugar content, and verify its performance. Results: The proposed method could achieve rapid and non-destructive detection of kiwifruit …

Walnut Defect Detection Based On Improved Elm And Computer Vision, Xu Jie, Liu Chang

Food and Machinery

Objective: To address the issues of low accuracy and poor efficiency in walnut defect detection among existing food production enterprises. Methods: Proposed a fast non-destructive detection method for walnut defects that combined improved extreme learning machines and computer vision. Collected most of the surface image information of walnuts through computer vision, preprocess the image through Gaussian filtering, optimize color and texture features through iterative and information preserving variable methods, finally, by improving the butterfly algorithm to optimize the parameters of the Extreme Learning Machine （random weights and deviations）, fast non-destructive detection of walnut defects could be achieved, and verify the …

Comparative Analysis Of Spectra And Modeling Of Different Cross-Diameter Orah Mandarin Based On Spectral Transformation Algorithm, Hu Feng, Gong Zhongliang, Wen Tao, Dai Xingyong, Tang Ziye

Food and Machinery

Objective: To reduce the influence of transverse diameter of Mandarin orange on the prediction of sugar content. Methods: 132 citrus were divided into three groups according to transverse diameter: small （65～70 mm）, medium （70～75 mm） and large （75～80 mm）. After collecting the transverse diameter spectrum of all citrus, the spectrum information and transverse diameter information of citrus were synthesized by spectral transformation algorithm, and the spectrum of different sizes of citrus was converted to the same transverse diameter datum. The pre-correction and post-correction spectra were respectively preprocessed, divided by target symbiotic distance algorithm （SPXY）, screened by competitive adaptive weight sampling …

Lightweight Banana Ripeness Detection Based On Improved Alexnet, Jiang Yu, Wang Lingmin

Food and Machinery

Objective: To obtain a lightweight Mini-Alexnet banana ripeness grading model and apply it to Android mobile devices. Methods: Based on the external characteristics of bananas with different ripeness, the Alexnet network model was restructured, part of the convolutional layer was deleted, and the global average pooling was used instead of the full connection layer to reduce the model parameters and required memory. A larger convolutional kernel was replaced to extract the global characteristics of the banana skin to achieve an improved lightweight Mini-Alexnet network model. Then the Mini-Alexnet network model was deployed as Android mobile APP, and its feasibility and …

Study On The Mechanism Of Boschniakia Rossica Polysaccharide Inhibiting The Activation Of Inflammasomes And Pyroptosis In J774a.1 Macrophages, Zhang Tian, Xu Hui, Quan Jishu, Jin Aihua

Food and Machinery

Objective: This study aimed to investigate the inhibitory effect of Boschniakia rossica polysaccharides （BRPS） on the activation of inflammasome and pyroptosis of J774A.1 macrophages induced by lipopolysaccharide （LPS） and adenosine triphosphate （ATP）. Methods: The nucleotide-binding oligomerization domain-like receptor protein 3 （NLRP3） inflammasome model was established by stimulating J774A.1 macrophages with LPS and ATP. Cell viability was detected using the cell counting kit-8, the release of lactate dehydrogenase （LDH） was tested with the microplate method, the cellular level of NLRP3 and cell membrane damage were detected using the immunofluorescence staining method, and the secretion of interleukin-1β （IL-1β） was …

Optimization Of Cellulase-Assisted Extraction Of Volatile Oil From Acorus Tatarinowii And Study On Its Inhibitory Components On Gaba-T Activity, Fang Chengwei, Ma Xiuping, Chen Liang, Li Qian

Food and Machinery

Objective: This study aimed to establish a hollow fiber ligand fishing method for screening γ- Aminobutyrate transaminase (GABA-T) inhibitory component from the volatile oil of Acorus tatarinowii. Methods: Based on optimizing the enzyme assisted extraction process of volatile oil from A. tatarinowii using response surface methodology, GABA-T solution was injected into the hollow fiber lumen and used as a "bait" to fish out the potential active components in A. tatarinowii volatile oil, and its GABA-T inhibitory activity was verified. Results: The results showed that the optimum extraction conditions of volatile oil from A. tatarinowii were as follows: cellulase content …