Food Biotechnology Commons^™

Research Progress Of Nanomaterials In The Rapid Detection Of Heavy Metal Ions In Food, Wang Yiping, Su Shufang, Yuan Yalan, Mao Ning

Food and Machinery

Nanomaterials have been widely used in the detection of heavy metal ions in food due to their large specific surface area, good stability, special structure, easy modification, and good biocompatibility. This review focuses on the research and application status of nanomaterials such as carbon nanotubes, graphene, carbon quantum dots, metal-organic frameworks and nanoenzymes in the detection of heavy metal ions in food. The challenges and application prospects of nanomaterials in the detection of heavy metal ions in food are also discussed.

Research Progress In The Detection Technology Of Chloramphenicol Residues In Animal Derived Food, Yuan Zhe, Peng Maomin, Liu Li, Peng Xitian, Xia Hong

Food and Machinery

Chloramphenicol is a widely used bactericide and highly effective antibiotic in aquaculture and livestock breeding. To protect human health, detecting chloramphenicol residues in animal-derived food products has become increasingly important. This review introduces the properties of chloramphenicol, the limit standards, and the hazards of residual chloramphenicol in animal-derived food products. Furthermore, it describes commonly used chloramphenicol detection methods. In addition, the review thoroughly analyzes and summarizes the advantages and disadvantages of different detection methods, outlining methods to improve the efficiency and precision of chloramphenicol detection to ensure the quality and safety of animal-derived food products. Finally, the prospects for developing …

Research On Image Recognition Of Cracked Eggs Based On Convolutional Neural Network Model, Li Shu, Tang Mengdi, Tong Siyuan, Sun Ke

Food and Machinery

Objective: In order to improve the accuracy and operating efficiency of the egg crack detection method based on computer vision. Methods: Used poultry egg simulation impact equipment to obtain cracked eggs, and collected images of cracked eggs and intact eggs from different angles through egg dynamic image acquisition equipment. Then, the YOLO-v5, ResNet and SuffleNet models for cracked egg image recognition were established for the original and the preprocessed egg images, respectively. After that, the recognition accuracy and the adaptability for original egg images recognition of different models were compared. Results: The YOLO-v5, ResNet and SuffleNet models could effectively identify …

A Fluorescence Elisa Based On Dsdna-Cuncs For The Detection Of E. Coli O157:H7 In Milk, Cao Wenkai, Shan Shan, Liu Daofeng, Peng Juan, Xing Keyu, Lai Weihua

Food and Machinery

Objective: This study aimed to developed a novel fluorescent enzyme-linked immunosorbent assay method for the sensitive detection of E. coli O157:H7 in milk samples. Methods: The pyrophosphate-Cu²⁺ coordination complex was hydrolyzed by alkaline phosphatase, and Cu²⁺ was released from copper nanoclusters as signal reporting probes. Key factors such as Cu²⁺ concentration, pyrophosphate concentration, and DNA template concentration and length were optimized. Results: Under the control of the optimized conditions, the method exhibited a good linear relationship (R²=0.980 8) with the target E. coli O157:H7 in the range of 5×10⁴~1×10⁸ CFU/mL. The …

Rutin Detection By Electrochemical Sensor Based On Fe3O4@Tio2@Au, Zhou Hong-Yan, Song Yun

Food and Machinery

Objective: A novel electrochemical sensor with high sensitivity was developed for the quantitative analysis of rutin in tartary buckwheat. Methods: A layer of titanium dioxide was loaded on the surface of ferric oxide nano materials (Fe₃O₄) to prepare core-shell Fe₃O₄@titanium dioxide (Fe₃O₄@TiO₂) nano composites, and nano gold (Au) was loaded on its surface for preparing a new type of Fe₃O₄@TiO₂@Au nanocomposites. Then an electrochemical sensor was constructed for quantitative analysis of rutin. The optimal working conditions of the sensor were …

Development Of A Matrix Reference Material For Amoxicillin In Lyophilized Bovine Milk, Liu Tian-He, Wei Ning, Wu Cai-Hong, Zhang Yu, Liu Qiu-Hui

Food and Machinery

Objective： This study aimed to provide the basis of quantity traceability for the analysis of amoxicillin residues in milk. At the same time, it can also provide quantity standards for ensuring the effective implementation of amoxicillin monitoring in milk and combating the abuse of amoxicillin in the country and regions. Methods： Two healthy middle-class dairy cows were injected with amoxicillin intramuscularly. After the natural metabolism of the drug for a period of time, the milk containing the target drug amoxicillin was collected and made into milk matrix reference material containing amoxicillin by vacuum freeze-drying technology. The uniformity test, stability test, …

Application Of Immunoassay In The Detection Of Amantadine Residues, Wang Lin, Wang Yao, Hu Xiao-Fei, Wang Cheng-Bin, Wu Jia-Bei

Food and Machinery

This review summarized and analyzed the immunoassay techniques, such as enzyme-linked immunosorbent assay, immunochromatographic assay, and chemiluminescence immunoassay, which have been applied in the detection of amantadine residue, and provides an outlook on their development.

Progress In The Application Of Nano-Antibody In The Detection Of Small Molecular Contaminants In Food, Jin Ping, Ding Hong-Liu, Jin Xiao-Hong, Shen Qi-Liang, Fan Chun-Yan

Food and Machinery

The structure and characteristics of nano-antibody and their applications in the detection of mycotoxins, pesticide and veterinary drug residues, small molecule toxic substances and other small molecule pollutants were introduced, and the development direction of nano-antibody in biological detection technology and diagnosis research was prospected.

Application Of Flow Cytometry In Microbial Detection Of Dairy Products, Qi Yan, You Chun-Ping

Food and Machinery

The principles and structures of flow cytometer, the methods and applications of flow cytometry in microbial detection of dairy products were introduced in this review, and the future research direction was prospected.

Progress On Detection Methods Of Main Mycotoxins In Food, Hang Yuan, Tong-Ying Ding

Food and Machinery

This review focused on the situation and toxicity of aflatoxin, deoxynivalenol, and alternaria toxin contaminated food, and the current commonly used mycotoxin detection methods at domestic and abroad were summarized, with the advantages and disadvantages of each method being analyzed and compred. Finally, the development direction of mycotoxin detection method in the future was prospected.

Salting-Out Assisted Liquid-Liquid Extraction Coupled With Hplc-Ms/Ms For Determination Of 13 Kinds Of Bisphenols And Alkyl Phenol Compounds In Liquid Dairy Products, Jiang Kan, Zhang Hui, Cao Hui, Wang Jin, Zhou Xue-Jun, Li Xiao-Yan

Food and Machinery

Objective: An analytical method was established for determination of 13 kinds of bisphenols and alkylphenol compounds in liquid dairy products by salting-out-assisted liquid-liquid extraction coupled with HPLC-MS/MS. Methods: The salting-out-assisted liquid-liquid extraction pre-treatment method was adopted, and the detection was performed in the mode of negative ion source multiple reaction monitoring (MRM) by triple quadrupole mass spectrometry, and quantitative analysis was performed by matrix external standard method. Besides, the effect of extraction solvent type, selection and the usage of salting-out agents on the recoveries were studied and the performance of the method was evaluated. Results: The liquid …

Research Progress On The Application Of Composite Hydrogel In Food, Wang Shao-Qing, Mu Ming-Wei, Zhang Bo, Deng Na, Wang Jian-Hui

Food and Machinery

At present, most hydrogels used in the food industry are natural polymers, with poor mechanical properties and high moisture content, which is easy to be contaminated by microorganisms, making it difficult to meet the use needs. In recent years, new composite hydrogels loaded with active substances prepared from natural and synthetic polymers or modified natural polymers have been increasingly used in the food field..Starting from the preparation method of composite hydrogels, this paper introduces its application status in food packaging materials, fat and starch substitution, bioactive molecule delivery, rapid detection of microorganisms, and prospects the development trend of composite hydrogels …

The Research On Detection Methods For Fat In The Fermented Milk Added With The Cmc-Na, Liu Fang-Fang, Xue Min-Min, Wu Qian, Peng Cheng-Cheng, Deng Ling-Yun

Food and Machinery

Objective: Improving the accuracy of the determination of fat in CMC-Na fermented milk. Methods: The national standard method-alkali hydrolysis method was used to determine the fat contained in CMC-Na fermented milk. By optimizing the key parameters in the alkali hydrolysis method, hydrolysis of hydrolysis instead of ammonia hydrolysis, and adding metal ions to the alkali hydrolysis method, the suitable fat containing CMC-Na fermented milk was explored. Results: ① With the increase of CMC-Na content, the lower the fat result in CMC-Na fermented milk measured by alkaline hydrolysis; ② The change of the four key conditions, such as the ammonia volume, …

Construction Of Lead (Ii) Ion Detection Method Based On Regulation Of Thiacyanine Self-Assembly By Nucleic Acid Aptamer, Zhao Han, Yu Li-Jia, Wu Zhi-Sheng, Zhang Ying-Lin, Zhang Hua-Wei, Shi Xiao-Dong

Food and Machinery

In this paper, regulation of self-assembly properties of thiacyanine dye (Dye2) by nucleic acid aptamer (T30695) was used to construct a specific recognition unit for detecting Pb²⁺. G-quadruplexes were formed to interact with dye 2 monomers, which could disassemble dimers of Dye2 into monomer, in the presence of Pb²⁺. The absorption vales at 422 nm via 445 nm was used as a specific recognition signal to achieve the purpose of specificity and high sensitivity identification of Pb²⁺ only by UV-vis spectra. This system has a good linear relationship from 0.25 to 10.00 μmol/L (R^{2 …}

Egg Crack Detection Based On Improved Particle Swarm Optimization, Zhang Jian, Cui Ying-Jie

Food and Machinery

Aiming at the problems of complexity and minuteness of egg crack detection, the improved particle swarm optimization algorithm is proposed in order to improve the detection effect. Firstly, the inertia weight was adjusted with cosine function, having the large value in the early stage and small value in the later stage. Secondly, particle reverse learning was prevented from falling into the extreme value trap, and the optimization was improved efficiently. Thirdly, adaptive threshold was segmented the egg image, variable median filter window was removed the dark spots on the egg image surface, and incomplete Beta function was enhanced the image. …

Parameter Design And Experiment Of Spectral Detection Tray For Orange Fruit Quality Based On Trace Pro Simulation, Zhang Qian-Qian, Xu Sai, Lu Hua-Zhong

Food and Machinery

In order to realize the visible/near infrared spectroscopy nondestructive testing of the internal quality of orange fruits, Trace pro software is used to carry out optical simulation analysis on the designed orange fruit online testing and conveying tray model. referring to the luminance/illuminance value in the simulation result, the tray model with higher value is processed in kind, and is tested and verified by combining with the actual spectrum testing platform.The simulation results show that the optimal shape parameters of the achievement transfer tray were: outer diameter 80 mm, inner transverse diameter 55 mm, inner longitudinal diameter 50 mm, and …

Evaluation Of Qualitative Food Allergen Detection Methods And Cleaning Validation Approaches, Rachel C. Courtney

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Allergen control plans are increasingly used by the food industry to prevent allergen cross-contact and validation of these plans involves methods to detect allergen protein residues. A commonly used rapid allergen detection method is lateral flow devices, although research about their validation is lacking. The objective of this research was to investigate lateral flow devices, their specificity and sensitivity to milk proteins and milk-derived ingredients, swabbing conditions, and applications in cleaning validation.

Several lateral flow devices advertised to detect total milk did not detect whey proteins or whey-derived ingredients. The overload level of the kits was highly variable (ranging from …

Detection Of Genetically Modified Foods, Olivia Boyce

Masters

In recent years, Genetically Modified (GM) foods have become increasingly common on our supermarket shelves. Consumer concerns regarding their safety have prompted codes of practice and legislation requiring labelling of all GM-food-containing products. Labelling requires some means of verification. There is no simple means of detecting GM food and until recently, there were no tests available. The object of this study was to develop a simple, rapid and user-friendly method of detecting genetically modified foods. The study concentrated on the detection of GM tomatoes, using a commercially available tomato paste. The method of choice was the Polymerase Chain Reaction (PCR) …