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Full-Text Articles in Food Biotechnology
Rapid Determination Of 81 Illegal Weight Loss Additives In Tablet Candy By Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry, Li Hongli, Su Min, Wu Yanlei, Zhang Mian, Wang Yuqi, Zhou Chunjie, Tao Juan
Rapid Determination Of 81 Illegal Weight Loss Additives In Tablet Candy By Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry, Li Hongli, Su Min, Wu Yanlei, Zhang Mian, Wang Yuqi, Zhou Chunjie, Tao Juan
Food and Machinery
[Objective] To establish a rapid detection method for 81 illegal weight loss additives in tablet candy using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). [Methods] Samples were ultrasonically extracted with 80% methanol-water (containing 0.1% formic acid), separated on a Waters ACQUITY UPLC HSS T3 column, and eluted with a gradient of methanol and 0.1% formic acid water solution. Detection was performed using multiple reaction monitoring (MRM) with simultaneous scanning in positive and negative electrospray ionization modes, and quantification was carried out using the external standard method. [Results] The 81 target analytes showed good linear relationships within their respective mass concentration ranges, …
Detection Of Apple Freshness During Storage Based On Zigbee Network And Pls-Da, Huang Haiyang, Qu Liqiu, Ma Yunlong
Detection Of Apple Freshness During Storage Based On Zigbee Network And Pls-Da, Huang Haiyang, Qu Liqiu, Ma Yunlong
Food and Machinery
[Objective] To address the long detection cycles, complex operation, and destructive sampling in apple freshness detection, a rapid and non-destructive detection method based on Zigbee wireless sensor network and partial least squares-discriminant analysis (PLS-DA) is proposed. [Methods] With Red Fuji apples as the research object, a gas sensor array acquisition system based on the Zigbee protocol is designed to monitor the concentration changes of four characteristic gases (ethanol, ethylene, oxygen, and carbon dioxide) during apple storage in real time. Least squares filtering is employed to preprocess the raw data, and the stable value, mean value, and maximum value are extracted …
Rapid Detection Of Viable Salmonella Based On Specific Phage Infection Combined With Fluorescent Rpa, Liu Daofeng, Zhai Yujie, Tan Yucheng, Shan Shan, Lu Jun, Zhong Ziqing
Rapid Detection Of Viable Salmonella Based On Specific Phage Infection Combined With Fluorescent Rpa, Liu Daofeng, Zhai Yujie, Tan Yucheng, Shan Shan, Lu Jun, Zhong Ziqing
Food and Machinery
[Objective] To establish a rapid detection method for viable Salmonella based on specific phage infection and recombinase polymerase amplification (RPA) in response to the key challenge that traditional molecular biological detection methods have difficulty in conveniently distinguishing between viable and non-viable foodborne pathogens. [Methods] Taking advantage of the characteristic that O-1 phage only proliferates in viable bacteria, the phage is added to the enriched sample. After further incubation, total DNA is extracted. Fluorescent RPA is used to determine whether the phage has significantly proliferated, so as to judge the presence of viable Salmonella in the original sample. [Results] After optimization, …
Establishment And Application Of A Uplc-Ms/Ms Method For Simultaneous Determination Of 39 Veterinary Drugs And Their Metabolites In Black Bone Chicken, Jiawei Du, Yongqin Li, Shuangyuan Jin, Xueyan Wang, Hongwu Wen, Xuelian Guo, Hao Li, Lihua Xu
Establishment And Application Of A Uplc-Ms/Ms Method For Simultaneous Determination Of 39 Veterinary Drugs And Their Metabolites In Black Bone Chicken, Jiawei Du, Yongqin Li, Shuangyuan Jin, Xueyan Wang, Hongwu Wen, Xuelian Guo, Hao Li, Lihua Xu
Journal of Food Science and Biotechnology
[Objective ] This study aims to comprehensively investigate the risk of veterinary drug residues in black bone chicken.[Method ] A method for rapid and simultaneous analysis of 39 veterinary drugs and their metabolites in three categories was established based on low-temperature ultrasonic extraction,solid-phase extraction pretreatment,and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ).The samples were extracted by Mcllvaine-Na 2EDTA and phosphate buffer via ultrasonic treatment at low temperatures,purified by Oasis HLB solid-phase extraction column,separated by T 3 chromatographic column,and quantified by the matrix matching external standard method.[Result ] The 39 veterinary drugs and their metabolites showed good linear relationships in …
Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li
Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the detection specificity and sensitivity for Escherichia coli (E.coli ) O157:H7.[Method ] A high ly specific and sensitive method for the detection of E.coli O157:H7 was established based on magnetic separation and signal amplification strategies combined with surface-enhanced Raman spectroscopy (SERS ).Aptamers were used as recognition elements and combined with magnetic beads and polystyrene (PS) microspheres to construct a separation probe (MB@Apt ) and a signal amplification Raman tag (4-MBN@PS@Apt ),respectively.[Result ] In the presence of E.coli O157:H7,MB@Apt and 4-MBN@PS@Apt formed a “sandwich ” structured composite (MB@Apt/ E.coli O157:H7/4-MBN@PS@Apt ),which could be rapidly …
Rapid Identification Of Drug Resistance Of Escherichia Coli Based On Electrochemical Square Wave Voltammetry Of Resazurin, Qi Chuchu, Lu Lisheng, Ren Jiali, Zhou Kai, Zhang Xinfang, Zhang Ziying
Rapid Identification Of Drug Resistance Of Escherichia Coli Based On Electrochemical Square Wave Voltammetry Of Resazurin, Qi Chuchu, Lu Lisheng, Ren Jiali, Zhou Kai, Zhang Xinfang, Zhang Ziying
Food and Machinery
Objective: To evaluate the antibiotic resistance of five foodborne Escherichia coli clinical isolates to ciprofloxacin, gentamicin and ampicillin. Methods: Based on the theory of electron transfer during bacterial energy metabolism and the introduction of resazurin as a redox probe, an electrochemical square wave voltammetry （SWV） method based on bladed tensin-mediated rapid identification of E. coli drug resistance was established. Results: The electrochemical square wave voltammetry values of E. coli at different concentrations mediated by resazurin were determined, and there was a good linear relationship between the E. coli concentration and the oxidized peak current （I） of resazurin in …
Application Of Surface-Enhanced Raman Spectroscopy In Food Safety Detection, Wang Liuliu, Sun Fangtao
Application Of Surface-Enhanced Raman Spectroscopy In Food Safety Detection, Wang Liuliu, Sun Fangtao
Food and Machinery
Based on Raman scattering phenomenon, surface-enhanced Raman spectroscopy （SERS） detection technology enhances "fingerprint" information of tested substance by adsorbing it on the surface of SERS substrate to achieve trace detection. With the advantages of simple pre-processing, high sensitivity, simple operation, real-time non-destructive detection on site and so on, SERS analysis technology has attracted much attention in food safety rapid detection. The characteristics and limitations of various types of SERS substrates were introduced. The recent research on SERS detection technology in food safety detection was summarized in detail. Finally, this paper discussed the challenges of its commercial application, and prospected the …
Preparation And Application Of Thiabendazole Molecularly Imprinted Sensor Based On Composite Functional Monomers, You Xiao-Ting, Hu Jing, Zhang Dong-Dong, Tang Lu-Hua, Gao Wen-Hui
Preparation And Application Of Thiabendazole Molecularly Imprinted Sensor Based On Composite Functional Monomers, You Xiao-Ting, Hu Jing, Zhang Dong-Dong, Tang Lu-Hua, Gao Wen-Hui
Food and Machinery
Objective: This study aimed to rapidly detect the residual quantity of thiamendazole in food. Methods: O-aminophenol and o-phenylenediamine were screened as composite functional monomers by ultraviolet spectroscopy. The polymerization and elution conditions were studied by electrochemical analysis, and the performance of the sensor was evaluated. A rapid method for the determination of thiamendam residues in food was established. Results: Under the control of the optimal conditions, the imprinted sensor had the specific adsorption performance for thiamendam and its structural analogues, and the selectivity for thiamendam was the strongest. The linear relationship of this method ranged from 1.0×10-8 to 1.0×10 …
Design Of Quinoa Moisture Rapid Detector Based On Capacitance Method, Qian Xi, Zhi-Yong Zhang, Kai Yuan, Dong-Sheng Guo, Ying-Rui Wu
Design Of Quinoa Moisture Rapid Detector Based On Capacitance Method, Qian Xi, Zhi-Yong Zhang, Kai Yuan, Dong-Sheng Guo, Ying-Rui Wu
Food and Machinery
The effects of temperature (5~40 ℃), water content (10.14%~29.55%) and frequency (1~100 kHz) on quinoa capacitance were studied by LCR meter. Based on the multiple regression analysis method, and the optimal excitation frequency (21 kHz) and water content detection model for quinoa water content detection were determined. The minimum system board of MSP430F149 microcontroller, TFT touch-screen, AD5933 impedance measurement chip, self-made concentric circle capacitance sensor and DS18B20 digital temperature sensor were selected to complete the design of the quinoa moisture rapid detector. The accuracy test of the detector showed that the average relative error of quinoa water content test results …
Research Progress Of Triplex Nucleic Acid In Food Safety Detection, Sun Hai-Yan, Lei Yan-Li, Chen Wei-Ju, Quan Ke
Research Progress Of Triplex Nucleic Acid In Food Safety Detection, Sun Hai-Yan, Lei Yan-Li, Chen Wei-Ju, Quan Ke
Food and Machinery
This paper reviews the structure and stability of triplex nucleic acid, the progress of triplex nucleic acid-based fluorescence, electrochemical, colorimetric platform in detecting food borne hazards, and discusses the challenges and future development prospect of triplex nucleic acid-based detection technology in food safety field.
Progress In Food Safety Rapid Analysis Based On Deoxyribozyme, Zeng Yu, Chen Wei-Ju, Quan Ke, Lei Yan-Li
Progress In Food Safety Rapid Analysis Based On Deoxyribozyme, Zeng Yu, Chen Wei-Ju, Quan Ke, Lei Yan-Li
Food and Machinery
The article expounds the structure and activity of DNAzyme, and reviews the application progress of DNAzymes in the detection of heavy metal ions, pathogenic bacteria, microbial toxin and other illegal chemical components in food. The challenges faced by DNAzymes in the actual detection of food harmful substances are analyzed and summarized, and future development prospect in rapid and on-site detection are prospected.
Development Of Rapid Detection Technique For Potassium Content In Cigarette Paper, Chen Peng-Fei, Wang Si-Lu, Yu Sai-Bo, Peng Xing, Huang Jia-Ying, Xiao Yang, Xiao Zhong-Liang, Cao Zhong
Development Of Rapid Detection Technique For Potassium Content In Cigarette Paper, Chen Peng-Fei, Wang Si-Lu, Yu Sai-Bo, Peng Xing, Huang Jia-Ying, Xiao Yang, Xiao Zhong-Liang, Cao Zhong
Food and Machinery
Objective:A method for simple and rapid determination of potassium content in cigarette paper will be built for workshops of cigarette factory.Methods:An ion-selective electrode method was used by comparing with the pretreatment methods for cigarette paper samples and potential response behaviors of potassium ion-selective electrodes in different buffer solutions.Results: The potassium ion selective electrode possessed excellent response performance for K+in lithium acetate buffer solution (0.1 mol/L, pH 7.0) with a linear response range of 1.0×10-5~1.0×10-2mol/L, a slope of 55.79 mV/dec(25 ℃), and a lower limit of detection of 2.14×10-6mol/L. The average relative standard deviations of the K+selective …
Rapid Determination Of Erythrosine In Beverages By Uplc, Xie Sai
Rapid Determination Of Erythrosine In Beverages By Uplc, Xie Sai
Food and Machinery
Objective:In order to develop a method, an easy handling of samples and accurate and rapid detection of erythrosine by ultra-high performance liquid chromatography (UPLC) was established.Methods:The analysis was as follows: chromatographic column of ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7 μm), diode array detector with detection wavelength at 530 nm, column temperature at 40 ℃, mobile phase with acetonitrile and0.02 mol/Lammonium acetate solution (acetic acid to adjust pH 6.25), gradient elution including acetonitrile 0 min-5%, 2 min-25%, 3 min-45%,6 min-70%, 6.5 min-5% and 8 min-5%, flow rate at0.3 mL/min, and with injection volume of 5 μL. …
Research On Rapid Prediction Model Of Rice Moisture Content Based On Near Infrared Spectroscopy, Lu Du, Tang Jian-Bo, Jiang Tai-Ling, Chen Zhong-Ai, Pan Mu
Research On Rapid Prediction Model Of Rice Moisture Content Based On Near Infrared Spectroscopy, Lu Du, Tang Jian-Bo, Jiang Tai-Ling, Chen Zhong-Ai, Pan Mu
Food and Machinery
Objective:In order to established a non-destructive, rapid and efficient method for detecting the moisture content of rice. Methods:This study, 161 rice samples were collected from 5 different regions were studied by near infrared spectroscopy combined with stoichiometry. By eliminating abnormal spectra and preprocessing the spectra, the prediction model of rice moisture content was established by partial least squares regression. Results:15 abnormal spectrum samples were eliminated using the method of principal component analysis combined with mahalanobis distance. The best spectral pretreatment was to eliminate the constant offset. The prediction model R2CAL established in the training set was 0.994 …
Detection Of Aflatoxin B1 In Edible Oil By Electrochemical Sensor Based On Sno2/Sic Hollow Sphere Nano Chain, Wu Min-Fu, Li Sha, Xu Zhen-Lin, Sun Yuan-Ming
Detection Of Aflatoxin B1 In Edible Oil By Electrochemical Sensor Based On Sno2/Sic Hollow Sphere Nano Chain, Wu Min-Fu, Li Sha, Xu Zhen-Lin, Sun Yuan-Ming
Food and Machinery
SnO2/SiC hollow spheres, polyaniline nanowires, gold nanoparticles and other nano materials with good biocompatibility were modified on the electrode substrate and antibody probe to amplify the electrochemical signal, improve the detection sensitivity, an
Research Progress In Detection Of Estrogen Residues In Food By Biological Probes Based On Specific Molecular Recognition, Chen Ting-Ting, Wang Xin, Tao Xiao-Qi
Research Progress In Detection Of Estrogen Residues In Food By Biological Probes Based On Specific Molecular Recognition, Chen Ting-Ting, Wang Xin, Tao Xiao-Qi
Food and Machinery
In this study, the main detection methods based on specific molecular recognition of biological probes in recent years are reviewed, including enzyme-linked immunosorbent assay, lateral flow immunoassay and electrochemical biosensor. The advantages and disadvantages of each method are analyzed.