Biotechnology Commons^™

Extraction Of Cannabidiol From Hemp Leaf Using Supercritical Fluid Technique And Production Of Cannabidiol Emulsion Microencapsule By Freeze Drying, Saranya Jansamutr

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to extract, purify, formulate, and evaluate the bioactivities of cannabidiol (CBD) derived from hemp (Cannabis sativa L.) leaves. Supercritical carbon dioxide (SC-CO2) extraction at pressures of 1,600–1,800 psi, combined with flash chromatography, was employed to obtain CBD-rich extracts. Higher extraction pressure significantly increased both yield and CBD concentration (p ≤ 0.05), with 1,800 psi selected for further formulation. The extracts were developed into emulsions and microcapsules using freeze-drying and ß-cyclodextrin as an encapsulating agent to enhance chemical stability. Emulsions were formulated using MCT oil, Tween 20, and lecithin in various ratios including 9:5:5 (formula 1), 8:4:4 (formula …

Application Of Mycelia Inoculum Of Ectomycorrhizal Fungus Astraeus Odoratus For Dry Dipterocarp Forest Restoration And Inoculum Development By Mycelial Entrapment With Calcium Alginate, Yanisa Punsung

Chulalongkorn University Theses and Dissertations (Chula ETD)

Dry Dipterocarp Forests (DDFs) in Thailand face severe degradation, primarily from human-induced fires, which significantly diminishes ectomycorrhizal (ECM) fungal diversity and the productivity of valuable wild mushrooms, such as Astraeus odoratus. However, concrete guidelines for restoring ECM fungal diversity in these degraded areas are currently lacking. This research aimed to develop a sustainable approach to enhance ECM fungal diversity and facilitate the restoration of degraded DDFs by utilizing A. odoratus mycelia. Furthermore, we developed alginate-encapsulated A. odoratus mycelial beads to extend inoculum viability and applicability. The study was conducted at two degraded DDF sites: Ban Kor Thung and Ban Mae …

Fungal Activity In Fusarium Keratoplasticum Isolates With And Without Endohyphal Bacteria On Various Carbon Sources, Maya Brown

Honors College Theses

In recent years, the fungus Fusarium keratoplastic has been associated with failed sea turtle eggs. One fungal isolate collected from a failed egg collected on Wassaw Island, GA in 2020 contained endohyphal bacteria, bacteria living within the hyphae of the fungus. The type of endohyphal bacteria (EHB), as well as the relationship between the bacteria and fungal hosts, are currently unknown. Previous research on the same fungus and EHB, specifically Chitinophaga sp., discovered that EHB influenced the ability of the fungus to utilize carbon sources. Our research goal was to examine the metabolic relationship of Fusarium isolated from failed sea …

Sdgs And Circularity In Fashion Industry: Bruneian Perspective Based On Basic Values, Izzah Mohd Noor, Shahid Anjum

ASEAN Journal on Science and Technology for Development

The fashion industry is facing significant textile waste, with millions of textiles ending up in landfills annually. Overconsumption of textiles and clothes due to fast fashion trends has negative environmental impacts. Transitioning to a circular economy is crucial for minimizing environmental impact and addressing the Sustainable Development Goals (SDGs) in the United Nations' 2030 Agenda. This study investigates the adoption of Circular Economy practices and SDGs awareness in second-hand clothing. Using IBM SPSS Statistics Software and Ordinary Least Squares (OLS) regression, the study models consumer behaviors, and attitudes by investigating the influence of four key components from Schwartz's theory of …

Factors Influencing Consumers' Intention To Use Over-The-Top (Ott) Services: A Study In Brunei Darussalam, Hjh Faralisa Hj Zulfikri, Heru Susanto

ASEAN Journal on Science and Technology for Development

The market for over-the-top (OTT) services has witnessed significant growth and transformation, presenting new opportunities and challenges to service providers. This study aims to explore the factors that influence consumers' intention to use OTT services, focusing on the context of Brunei Darussalam. Drawing on the Technology Acceptance Model (TAM) as a theoretical framework, this study investigates the impact of perceived ease of use, perceived usefulness, innovativeness, service quality, information seeking, and entertainment on consumers' intention to use OTT services. A quantitative research approach was adopted and data were collected through an online survey conducted among the general public in Brunei …

Exploring The Impact Of Vented Packaging Configurations On Airflow Patterns And Cooling Consistency For Muskmelons In A Cold Storage Environment, Nur Wafidah Roslan, Namasivayam Navaranjan, Zohrah Sulaiman, Naseem Uddin

ASEAN Journal on Science and Technology for Development

The cooling effect of different vented packaging designs was investigated for packed muskmelons fruits in a commercial cold storage condition. Computational fluid dynamics (CFD) simulation and experiments were used. The airflow pattern, heat transfer and temperature profiles were predicted from simulation and analysed. The CFD results reveal an uneven and low efficient airflow distribution inside a commercially used vented packaging (Box A). This resulted slow cooling of the fruits in Box A. Two newly designed packaging (Box B and Box C) showed better airflow distribution around the fruits and cooled them faster than Box A. The design of Box C …

Smes’ Perspectives On The Benefits And Challenges Of Halal Certificate Application In Brunei Darussalam, Kamariah Ismail, Syahnur Farhana Haji Shahlehi, Vivi Nabilah Shaya

ASEAN Journal on Science and Technology for Development

The knowledge and research on SMEs and Halal-related studies in Brunei Darussalam are scant. Guided by the principles of Resource-Based View theory, this qualitative study aims to identify the benefits and challenges of Halal certificate application from the perspectives of Bruneian Halal food SMEs, as they obtain the Brunei’s Halal certificate and label. Based on the purposive and snowball sampling, four semi-structured interviews were conducted with business owners and managers in the Halal food industry. Government officials were also in-terviewed to enhance the findings. Thematic analysis, supplemented by secondary data, was employed for a comprehensive understanding. Four themes emerged, covering …

Word Prediction Using Dynamic Skip Connections Along With Arabert And Lstm In Arabic Language, Ahad Almalki, Faris Kateb, Rayan Mosli

ASEAN Journal on Science and Technology for Development

Natural Language Generation (NLG) plays a crucial role in modern digital tools, including chatbots, virtual support, content suggestions, and tailored marketing, making bots more responsive and reducing the need for human staff. While there's much research on NLG for languages like English, languages like Arabic, Urdu, and Chinese still face challenges. This study examines Arabic NLG's unique aspects, dialects, and word variations. With around 420 million Arabic speakers globally, it's crucial to advance NLG for this language. We compared three models: Long Short-Term Memory (LSTM), a mix of Bidirectional Encoder Representations from Transformers (BERT) and LSTM, and a version that …

Sustainability Innovation, Firm Competitiveness, Export Performance, And Sales Growth: A System Dynamics Approach, Budi Harsanto, Joval Ifghaniyafi Farras, Egi Arvian Firmansyah

ASEAN Journal on Science and Technology for Development

In recent times, the interplay between sustainability innovation, firm competitiveness, export performance, and sales growth has garnered significant attention. Research on sustainable innovation's effects on firm competitiveness, export performance, and sales growth remains limited, especially concerning specific contexts like Halal Micro, Small, and Medium Enterprises (MSMEs). The study proposes to bridge this gap by developing a System Dynamics model that considers firm competitiveness, sales growth, export performance, and sustainable innovation. This research uses the system dynamic method which is carried out in four stages. The first stage, analyzing the system mechanism for sustainable innovation, the second stage, building correlations between …

Enhancing Phishing Attack Detection Using Explainable Ai: Trends And Innovations, Bhagyashree D. Shendkar, Pankaj R. Chandre, Sulochana Sagar Madachane, Nikita Kulkarni, Sayalee Deshmukh

ASEAN Journal on Science and Technology for Development

In the context of escalating cyber threats, phishing attacks continue to pose significant challenges to cybersecurity measures, exploiting human vulnerabilities to gain unauthorized access to sensitive information. Innovative solutions are required because traditional detection methods frequently fail to identify and mitigate these assaults successfully. To improve phishing attack detection, this study makes use of Explainable Artificial Intelligence (AI) tools, which provide decision-making processes with transparency and prediction accuracy. The paper begins by outlining the pervasive nature of phishing attacks in today's digital landscape, emphasizing their detrimental impact on individuals, organizations, and society at large. It then discusses the limitations of …

Practical Challenges In Delivering Dsrna For Woody Plant Protection, Zachary Bragg

Theses and Dissertations--Entomology

Unprecedented weather events associated with climate change, coupled with extensive anthropogenic stresses, have reduced the effectiveness of traditional forest pest management approaches, which can no longer keep pace with invasive species overwhelming naïve landscapes and native pests experiencing eruptive and expansive outbreaks. Double-stranded RNA-(dsRNA) mediated gene silencing, a type of RNA interference (RNAi), has been coopted for use as a biopesticide against a multitude of agricultural and horticultural pests and could serve as a powerful tool for woody plant protection. While effective initiation of gene silencing and subsequent mortality have been demonstrated in multiple tree pests, effective and efficient methods …

Antioxidant And Antimicrobial Properties Of Yellow Mealworm (Tenebrio Molitor) Protein Concentrates And Hydrolysates Derived From Varied Dietary Regimens, Shyang-Pei Hong, Eng-Tong Phuah, Wey-Lim Wong, Yee-Ying Lee, Teck-Kim Tang, Ali Yassoralipour

ASEAN Journal on Science and Technology for Development

The larval stage of the darkling beetle, Tenebrio molitor, often referred to as the yellow mealworm, has received significant attention as a promising source of high-quality protein for human consumption due to its exceptional nutritional profile. This study focused on assessing the antioxidant and antimicrobial activities of protein concentrates and hydrolysates obtained from mealworm specimens raised under distinct dietary conditions. One cohort of mealworms was sustained on food waste comprising rice bran, milled corn, and carrots (self-bred) (SM), while another group was exclusively provided with rice bran (commercially fed) (CM). Enzymatic hydrolysis of mealworm protein was accomplished using protease enzymes …

Youth Resilience Toward Climate Smart Agriculture Technology Adoption In Brunei Darussalam, Rosita Hassan, Wida S. Suhaili, Beston F. Nore, Mohamad A. Basuni

ASEAN Journal on Science and Technology for Development

Climate change and food security are critical issues to be addressed, nationally and globally, to reach sustainable agricultural development goals to feed the growing human population. The great challenge is to adapt to the climate change and in the meantime increase food production with sustainable farming practices that require farmers to have a paradigm shift toward climate smart agricultural technology (CSAT). Encouraging farmers to adopt and implement CSAT have become a priority for governmental policy making programmes. The question is what are the measures that can be taken for Brunei to leapfrog into this technology bandwagon to revitalise farmers for …

Innovation Barriers Obstructing The Transfer Of Technology From University To Industry In Asean Developing Countries (Case Study On Brunei Darussalam), Rafiq Zaini, Kamariah Ismail

ASEAN Journal on Science and Technology for Development

This study aims to explore the barriers of innovation in one of ASEAN developing countries particularly Brunei Darussalam. This research focuses on 3 sectors which are from 1) the government of Brunei to 2) the higher education institutions particularly Universiti Teknologi Brunei to 3) industry sectors in Brunei Darussalam - primarily in the processes of technology transfer. Using the Triple Helix theory model, barriers were identified between consecutive sectors. In this study, qualitative research methods have been utilized (case studies using interview methods) and a total of 9 main barriers have been identified between the sectors from the literature review …

A Beacon Of Climate Change And Green Financing Toward Bank Stability Among Tiger Cub Economics, Mohd Razuan Abd Hishamuddin, Imbarine Bujang, Flicia Rimin, Irma Febriana Mimma Kebahyang

ASEAN Journal on Science and Technology for Development

Economic growth strongly depends on financial stability, given that the financial sector conducts most transactions in the real economy. Financial instability causes banking institutions to be wary about backing profitable businesses, resulting in a reluctance to fund such activities. Green financing is one of the measures to minimize banking instability. In general, the role of green financing would be to mitigate climate change risk and control the environment. Numerous studies have been conducted on an empirical model of the relationship between climate change and the financial stability of banking institutions. However, little emphasis is placed on the context of adopting …

The Interaction Of Maqasid Al-Shariah In The Relationship Between Esg And Firm Financial Performance, Dayang Nuradzlina Radin, Norhasimah Shaharuddin, Imbarine Bujang, Igo Febrianto Rijanto

ASEAN Journal on Science and Technology for Development

This paper aims to analyse the role of Maqasid Al-Shariah as a value-added factor in the implementation of ESG for firms' financial performance. Most publicly listed firms are encouraged to get involved in sustainability reporting; however, the adoption rate of ESG among non-financial firms seems unattractive. Therefore, this paper will evaluate the literature review using the systematic literature review approach to justify the role of Maqasid Al-Shariah in implementing ESG. The general conclusion suggested that ESG plays an essential role in financial performance despite the implementation focusing more on cost. The inclusion of Maqasid Al-Shariah in ESG believes that the …

Recent Advance In Biodegradable Packaging From Banana Plant Feedstock: A Comprehensive Review, M. Atiq Juani, Namasivayam Navaranjan

ASEAN Journal on Science and Technology for Development

This review focuses on recent advances in biodegradable packaging derived from banana plant waste, highlighting its pivotal role in addressing environmental concerns linked to conventional packaging materials. The shift toward sustainable packaging stems from the urgent need to combat plastic pollution, reduce reliance on non-renewable resources, and foster sustainability in the food industry. Banana plants, known for generating significant organic waste during cultivation and processing, provide a valuable source for developing biodegradable packaging. Researchers have successfully transformed banana waste into innovative, recyclable, and eco-friendly packaging solutions, promoting a circular economy. Biodegradable packaging, in contrast to traditional materials derived from fossil …

Unlocking Antioxidant Potential: Comparative Analysis Of Sembada 188 And Keladi Rice As Functional Ingredients In Novel Food Production, Mas Munira Rambli, Natasha Azamain, Eng-Tong Phuah

ASEAN Journal on Science and Technology for Development

Rice (Oryza sativa L.) is an essential crop that is a dietary staple for more than half of the world's population. There is currently a movement in consumer behaviour toward healthier food consumption, which is fuelling interest in functional diets like coloured rice. Sembada 188 and Keladi rice are underutilised in Brunei Darussalam and are expected to have functional qualities. A comparison of antioxidant properties between Sembada 188 and Keladi rice was performed in this study. This research produced puffed rice treats prepared by steaming rice, washing, drying for 6-8 hours at 60℃, deep frying for 25 seconds at 180℃, …

Preliminary Study On The Effects Of Vinegar As Pre- Treatment For The Oven-Drying Of Pacific Yellowtail Emperor (Lethrinus Atkinsoni) Fillets, Nurisa A. Suhaili, Rafael S. Jamih, Normina A. Abubakar, Jaro O. Ajik, Merilyn Q. Amlani

ASEAN Journal on Science and Technology for Development

This study aimed to assess the impact of various vinegar compositions used as pre-treatment for Pacific Yellowtail Emperor (PYE) fillets during the oven-drying process, with a focus on moisture content and optimal drying conditions. Two types of commercially available vinegar, Superior Vinegar and Datu Puti Vinegar, were compared, and different drying temperatures were evaluated. The investigation revealed that the drying temperature significantly influenced the moisture content of the dried PYE fillets. Among the tested temperatures (40°C, 60°C, and 80°C), the most favorable outcome in terms of moisture content was achieved when fillets were dried at 80°C for a duration of …

Byproducts Of Tropical Marine Processing As A Candidate For Industrial Food Ingredients: An Appraisal On Macro And Micronutrients, Abdul Aziz Jaziri, Hajariah Hasanuddin, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Masmunira Rambli, Nurul Huda

ASEAN Journal on Science and Technology for Development

Tropical marine fish is a main raw material for surimi processing plants, including golden threadfin bream (Nemipterus virgatus Houttuyn, 1782) and swallowtail dwarf monocle bream (Parascolopsis eriomma Jordan & Richardson, 1909). After processing, more than 60% of fish are considered as byproducts (i.e., head, viscera, skin, bones, fins and scale), and tend to discard due to poor-utilization. Hence, this paper aims to evaluate macro- and micro-nutrients derived from golden threadfin bream (GTB) and swallowtail dwarf monocle bream (SDMB). The higher content of protein (25.31 ± 0.81%) was observed in the skin part of GTB, followed by the flesh (21.26 ± …

Protein Content Analysis On Brunei Delicacies: Kelupis Sulang And Sulang Fruit, Siti Nadia Saifullzan, Jhoorhanah A. Halim, Mas M. Rambli, Aida M. Basri

ASEAN Journal on Science and Technology for Development

Traditional Bruneian delicacy which are often reserved for special occasions; called the kelupis Sulang made from glutinous rice, coconut milk, and the flesh of the Brunei Sulang fruit’s protein content were studied. The protein content of both kelupis and the sulang fruit were examined using both qualitative and protein concentration analyses. The analysis was performed using the Xanthoproteic test, Ninhydrin test, Sulphur Reduction test, and the Biuret test. The readings were obtained from the UV-Visible Spectrophotometry and revealed an average protein concentration of 21 mg/ml in aqueous extracts of kelupis sulang and 26.8 mg/ml in aqueous suspension of kelupis sulang …

Mobile Robot Adhesion Methodology And Development Of An Automatous Robot Module, Lauren Baird, Zachariah Stone, Madison Lemons, Jonathon Moody

Williams Honors College, Honors Research Projects

Due to the increasing availability of space travel as not only a scientific exploration but a commercial exploration, there is a need for an onsite repair station that can be deployed in the event of aircraft maintenance, damage, or failure. We have been tasked with researching and creating a prototype of an automatous robot that can be attached to a spacecraft body, move along the surface while avoiding obstacles, scan for damage, 3D print a repair piece, and then make the repair, all without the need of direct human input. Our team, as will be discussed throughout, was tasked with …

Cyp6g2 Is The Major P450 Epoxidase Responsible For Juvenile Hormone Biosynthesis In Drosophila Melanogaster, Qiangqiang Jia, Liu Yang, Jiamin Wen, Suning Liu, Di Wen, Wei Luo, Weihua Wang, Subba R. Palli, Li Sheng

Entomology Faculty Publications

Background Juvenile hormones (JH) play crucial role in regulating development and reproduction in insects. The most common form of JH is JH III, derived from MF through epoxidation by CYP15 enzymes. However, in the higher dipterans, such as the fruitfly, Drosophila melanogaster, a bis-epoxide form of JHB3, accounted most of the JH detected. Moreover, these higher dipterans have lost the CYP15 gene from their genomes. As a result, the identity of the P450 epoxidase in the JH biosynthesis pathway in higher dipterans remains unknown.

Results In this study, we show that Cyp6g2 serves as the major JH epoxidase responsible for …

The Mechanoreceptor Piezo Is Required For Spermatogenesis In Bombyx Mori, Zhongjie Zhang, Xiaojing Liu, Bo Hu, Kai Chen, Ye Yu, Chenxin Sun, Dalin Zhu, Hua Bai, Subba Reddy Palli, Anjiang Tan

Entomology Faculty Publications

Background The animal sperm shows high diversity in morphology, components, and motility. In the lepidopteran model insect, the silkworm Bombyx mori, two types of sperm, including nucleate fertile eupyrene sperm and anucleate unfertile apyrene sperm, are generated. Apyrene sperm assists fertilization by facilitating the migration of eupyrene spermatozoa from the bursa copulatrix to the spermatheca. During spermatogenesis, eupyrene sperm bundles extrude the cytoplasm by peristaltic squeezing, while the nuclei of the apyrene sperm bundles are discarded with the same process, forming matured sperm.

Results In this study, we describe that a mechanoreceptor BmPiezo, the sole Piezo ortholog in B. mori, …

Impact Of Financial Management System On Organizational Accountability And Responsibility: A Study Of Corporate Entities, Nur Farhanah Binti Marjuni, Junaid M. Shaikh

ASEAN Journal on Science and Technology for Development

This research investigates the impact of Financial Management Systems (FMS) on organizational accountability and responsibility in Brunei's manufacturing sector, utilizing a mixed-methods approach that includes primary data collected through questionnaires distributed to 41 local manufacturing companies and supplemented by secondary data from other ASEAN countries for comparative analysis. The primary focus is to assess the awareness and significance of efficient FMS implementation. Findings demonstrate a substantial influence of FMS on organizational accountability and responsibility, supported by an analysis of primary and secondary data from the ASEAN region. Nevertheless, this study acknowledges limitations such as its exclusive focus on Brunei and …

Fostering Corporate Resilience: Assessing Risk Management Committee Effectiveness In Malaysia’S Telecommunication And Media Sector Pre- And During The Covid-19, Flicia Rimin, Siti Rahayu Beli, Anastasiah Harbi, Imbarine Bujang, Jamaliah Said

ASEAN Journal on Science and Technology for Development

The COVID-19 pandemic has significantly impacted organizations, underscoring the critical role of financial factors in determining performance. Maintaining a healthy financial stance during crises necessitates effective risk management, prompting the exploration of a Risk Management Committee (RMC) as a focal point for addressing risk issues and managing internal controls. A study spanning 2017 to 2021 assessed 11 listed companies in the Telecommunication and Media Sector, revealing that a combined RMC did not enhance financial performance, indicating its ineffectiveness during the crisis. The study advocates for a separate risk management committee due to the audit committee’s limitations in performing both functions. …

Contemporary Vs. Traditional Dna-Based Mutation Testing To Detect Hereditary Breast And Ovarian Cancer Syndrome (Hboc) In Women: A Meta Narrative Review, Hannah Olivia Cantu, Jacob Hipp, Nyela Y. Lopez, Duc Duy Nguyen

Research Methods Poster Session 2024

No abstract provided.

Effect Of An Mrna Vaccine On Liver-Associated Macrophages And The Immunogenicity Of Ag85b-Mrna Vaccine Against Tuberculosis, Chitsuda Pongma

Chulalongkorn University Theses and Dissertations (Chula ETD)

A new vaccine technology based on the delivery of mRNA has gained widespread acceptance due to the COVID-19 pandemic. The delivery of mRNA formulated with lipid nanoparticles (LNPs) elicits a robust immune response, activating both humoral and cell-mediated response. Depending on the LNP formula, mRNA encoding proteins can be detected in the liver upon intramuscular (IM) administration in mice. Thus, an mRNA vaccine present novel platform for new vaccines for infection diseases, including tuberculosis (TB). This study proposed two major goals to investigate the impact of an mRNA vaccine on innate immune cells in the liver and the use of …

Developing A Novel Gold Nanoparticle-Based Colorimetric Assay For The Detection Of Cytomegalovirus (Cmv) In Pediatric-Derived Urine Specimens, Sonam Gupta

Graduate Thesis and Dissertation 2023-2024

Cytomegalovirus (CMV) is a member of the Herpesviridae family and is known to infect people of all ages. In most cases, CMV infection is asymptomatic, and the virus is cleared from the host without showing any significant symptoms. However, 1 out of 200 babies are born with congenital CMV infection, which affects multiple organs, including the brain, liver, spleen, lung, and inner ear. One long-term health problem in 1 out 5 babies born with congenital CMV infection is hearing loss. The progression of CMV-associated hearing loss in the first two years of life may lead to developmental delays in language, …

Rare Variant Analyses Validate Known Als Genes In A Multi-Ethnic Population And Identifies Antxr2 As A Candidate In Pls, Tess D. Pottinger, Joshua E. Motelow, Gundula Povysil, Cristiane A. Martins Moreno, Zhong Ren, Hemali Phatnani, The New York Genome Center Als Sequencing Consortium, Timothy J. Aitman, Javier Santoyo‑Lopez, Scottish Genomes Partnership, Hiroshi Mitsumoto, Als Cosmos Study Group, Pls Cosmos Study Group, Gtac Investigators, David B. Goldstein, Matthew B. Harms

Neurology Faculty Publications

Background Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease affecting over 300,000 people world‑ wide. It is characterized by the progressive decline of the nervous system that leads to the weakening of muscles which impacts physical function. Approximately, 15% of individuals diagnosed with ALS have a known genetic variant that contributes to their disease. As therapies that slow or prevent symptoms continue to develop, such as antisense oligonucleotides, it is important to discover novel genes that could be targets for treatment. Additionally, as cohorts continue to grow, performing analyses in ALS subtypes, such as primary lateral sclerosis (PLS), becomes possible …