Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Plant Sciences (7)
- Biochemistry (3)
- Biochemistry, Biophysics, and Structural Biology (3)
- Genetics and Genomics (3)
- Medicine and Health Sciences (3)
-
- Microbiology (3)
- Cell and Developmental Biology (2)
- Chemistry (2)
- Genetics (2)
- Medical Biotechnology (2)
- Medical Sciences (2)
- Medical Specialties (2)
- Oncology (2)
- Pharmacology, Toxicology and Environmental Health (2)
- Physical Sciences and Mathematics (2)
- Plant Biology (2)
- Animal Sciences (1)
- Bacteriology (1)
- Biodiversity (1)
- Biological Phenomena, Cell Phenomena, and Immunity (1)
- Biomedical Engineering and Bioengineering (1)
- Botany (1)
- Cancer Biology (1)
- Engineering (1)
- Environmental Sciences (1)
- Immunology and Infectious Disease (1)
- Inorganic Chemistry (1)
- Molecular Genetics (1)
- Keyword
-
- Plant genetic transformation (2)
- A non-living algal biomass (1)
- Animal biotechnology--Research (1)
- Antibactertial property (1)
- Antibodies (1)
-
- Antioxidants (1)
- Aquatic plants (1)
- Bacteria--Biological control (1)
- Bacteria--Classification--Technique (1)
- Bioinorganic chemistry (1)
- Biotechnology (1)
- Cancer cells (1)
- Cancer--Chemoprevention (1)
- Carcinogenesis--Analysis (1)
- Cell (1)
- Cervix uteri--Cancer (1)
- Cytoplasmic inheritance (1)
- Development of near real-time assessment (1)
- Drug resistance in microorganisms (1)
- Fermentation (1)
- Fertilization (Biology) (1)
- Fertilization in vitro (1)
- Fungi--Malaysia--Biotechnology (1)
- Genetic transformation (1)
- Hematopoietic stem cells--Transplantation (1)
- Immunocytochemistry (1)
- Medical Biotechnology (1)
- Medicinal plants (1)
- Medicinal plants--Micropropagation (1)
- Micropropagation (1)
Articles 1 - 17 of 17
Full-Text Articles in Biotechnology
Development Of Near Real-Time Assessment System For Cancer Cells., Nahrizul Adib Kadri
Development Of Near Real-Time Assessment System For Cancer Cells., Nahrizul Adib Kadri
Student Works (2010-2019)
Dielectrophoresis (DEP) is an electrical phenomenon that occurs when a polarisable particle is placed in non-uniform electrical fields, generating DEP force (FDEP) that is dependent upon the electrophysiological make-up of the particle in use. In cell cytometry studies, DEP offers the advantage of conducting various cell manipulations in vitro without the need of specific surface markers. Its drawbacks, namely the time-consuming processes involved and the inability to conduct large batch cell assays, had limited its usage. This thesis presents the work that offered solution to the said obstacles, by developing a system capable of conducting DEP experiments within a very …
Factors Affecting Cytoplasmic And Nuclear Structures Of Mouse Gametes And Embryos Using Immunocytochemistry Technique., Nooraain Hashim
Factors Affecting Cytoplasmic And Nuclear Structures Of Mouse Gametes And Embryos Using Immunocytochemistry Technique., Nooraain Hashim
Student Works (2010-2019)
Experiment 1 was conducted with the main objective to establish ICC technique for morphological analysis of the mouse oocytes and embryos in local laboratory. Several tests were conducted to achieve specific aims which included; a) to study efficacy of the available instruments and system for detecting fluorescence signals from mouse oocytes, b) to compare efficiency of improved methods to reveal oocyte morphology. Fluorescence images of the meiotic spindles, cortical granules and nuclear materials were compared between sample preparations methods. The results showed that the methods employed were reliable to be used for detecting the meiotic spindle, cortical granules and nuclear …
Purification And Analysis Of Bacterial-And Plant-Derived Scfv Antibodies For The Detection Of Cucumber Mosaic Virus, Wong C.F.Y.
Purification And Analysis Of Bacterial-And Plant-Derived Scfv Antibodies For The Detection Of Cucumber Mosaic Virus, Wong C.F.Y.
Student Works (2010-2019)
abstract N/A
Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari
Biolistic Transformation Of Oil Palm Using The Phosphomannose Isomerase (Pmi) Gene As The Positive Selectable Marker., Bahariah Bohari
Student Works (2010-2019)
The selectable marker system based on the Escherichia coli phosphomannose isomerase (pmi) gene was adapted for genetic transformation of oil palm. This system makes use of the pmi gene that encodes phosphomannose isomerase, which converts mannose-6-phosphate to fructose-6-phosphate and uses mannose as the selection agent. This is to anticipate the future requirement of using non-antibiotic resistance genes for the commercialization of transgenic oil palm. The use of antibiotic or herbicide-based selection systems has caused much public concern due to inadequate knowledge of the agents’ impact on the environment and on human health. In this study, four transformation vectors, namely pMI3, …
Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah
Studies On The Bioactive Properties Of Selected Plant And Fermented Extracts., Saravana Kumar Sinniah
Student Works (2010-2019)
The aim of this study was to isolate protein/peptide and secondary metabolites from plant and fermented sources. The extracts were used to test for antimicrobial and antioxidant properties. In the current study protein/peptide extracts of Allium sativum showed promising results with a 15mm inhibition zone against Escherichia coli, 28mm against Staphylococcus aureus, 16.3mm against Bacillus cereus and 9mm against Pseudomonas aeruginosa. The minimum bacteriocidal concentration (MBC) results showed that Allium sativum protein/peptide extract is only bacteriostatic and not bacteriocidal. Andrographis paniculata showed the most promising result for the ethanolic extracts with an inhibition zone of 14mm against B. cereus, 11.5mm …
Estimation Of The Product Yield And Collection Efficiency Of Peripheral Blood Stem Cell Harvesting Using Peripheral Blood Cd34[+] Cells Quantification., Habsah Aziz
Student Works (2010-2019)
Peripheral blood stem cells (PBSC) have been used for transplantation since the early 1990s. CD34+ has been used as a marker of hematopoietic stem cells to determine the optimal timing for PBSC harvesting. However, a wide ranges of CD34+ cells cut-off values from 8/ul to 66/ul have been used to initiate PBSC harvesting in autologous patients. We performed here a study to determine the peripheral blood CD34+ cells cut-off value for the prediction of stem cell yield of 2 x106 CD34+ cells/kg body weight patients, to study the correlation between peripheral blood CD34+ cells and stem cell yield, to study …
Diversity And Antioxidant Activity Of Trametes Fr. In Malaysia., Noraswati Mohd Nor Rashid
Diversity And Antioxidant Activity Of Trametes Fr. In Malaysia., Noraswati Mohd Nor Rashid
Student Works (2010-2019)
Research on antioxidant properties as a natural antioxidant, particularly in polypores, have been widely done since the past decade. It is known that antioxidant capacity is essential in protecting organisms and cells from damage brought on by oxidative which is believed to be the cause for ageing and degenerative diseases. In this study, biodiversity and antioxidant activity of Malaysian Trametes spp. which is from the group of polypores were investigated. Polypores were collected in Peninsular Malaysia forests from the year 2006 until 2007. The species were identified by taxonomic keys based on macromorphology and micromorphology analysis. Trametes hirsuta, T. pocas, …
Cytotoxic And Anti-Human Papillomavirus Activites In Selected Vegetables., Maizatul Azmah Ab. Latiff
Cytotoxic And Anti-Human Papillomavirus Activites In Selected Vegetables., Maizatul Azmah Ab. Latiff
Student Works (2010-2019)
An appropriate way to control cancer is chemoprevention as a large number of compounds have been observed to prevent the occurrence of cancer and variety of mechanisms exists for producing such protective effects. Numerous epidemiological studies have shown that diets rich in vegetables are associated with a decreased risk of cancer. Chemoprevention involves the use of natural or laboratory made substances to prevent cancer or reduce risk cancer.The E6 oncoprotein from oncogenic Human papillomaviruses (HPVs) has exhibit functions in tumorigenesis, regulation of transcriptions and apoptosis. E6 product from oncogenic viral type like HPV 16 can bind the tumor suppressor p53 …
Synthesis, Characterization And Biological Activities Of Three Indole Schiff Bases And Their Copper And Nickel Complexes., Nazzatush Shimar Jamaludin
Synthesis, Characterization And Biological Activities Of Three Indole Schiff Bases And Their Copper And Nickel Complexes., Nazzatush Shimar Jamaludin
Student Works (2010-2019)
Three derivatives of indole-3-carboxaldehyde with indole-3-acetic hydrazide and their copper(II) and nickel(II) complexes were synthesized. The Schiff base compounds were varied on the substituent where each compound holds a hydrogen, chlorine and bromine atom on the 5th position of indole-3-carboxaldehyde. The structures of the ligands and their complexes were elucidated by various characterization methods. All compounds were screened for their biological effects on neuroprotective screening (in vitro), acute toxicity test (in vivo), ethanol-induced gastric ulcer (anti-ulcer) and glucose tolerance test (antidiabetic). All compounds except bromine-containing compounds were not dose dependent while compounds with bromine displayed significant results, acting as inhibitory …
Agrobacterium-Mediated Transformation Of Cryptocoryne Willisii With Gus And Mgfp 5 Reporter Genes / Wong Sher Ming, Wong Sher Ming
Agrobacterium-Mediated Transformation Of Cryptocoryne Willisii With Gus And Mgfp 5 Reporter Genes / Wong Sher Ming, Wong Sher Ming
Student Works (2010-2019)
In this study, a simple method using Murashige and Skoog basal medium (1962) supplemented with 6-BA alone was developed for direct multiple shoots induction from shoot base culture of C. willisii. Shoots proliferation and elongation were achieved on a single medium without the need of subculturing. The optimum concentration of 6-BA for direct shoots regeneration of C. willisii was investigated. It was found to be 6 mg/ L. Optimum media was then used for regenerate and propagate of transformed C. willisii. On the other hand, ransformation of C. willisii with Agrobacterium tumefaciens strain LBA4404 harbouring pCAMBIA1304 was performed. Effects on …
Application Of Pcr-Based Serogrouping Of Salmonella Enterica., Mohammed Elbagir Mohammed Elhassan Nori
Application Of Pcr-Based Serogrouping Of Salmonella Enterica., Mohammed Elbagir Mohammed Elhassan Nori
Student Works (2010-2019)
Serotyping is the most fundamental approach in epidemiological surveillance and outbreak investigations for Salmonella. Many multiplex PCR assays have been reported for serotyping Salmonella based on O and H antigens. The aim of this study was to apply and improve the approach in differentiation and serotyping Salmonella enterica strains by combination of sequential multiplex PCRs targeting the O, H and Vi antigens of Salmonella, and this achieved by working on 126 strains. All the strains were purified on BSA and produced black colonies of Salmonella. The strains were confirmed by PCR amplification gene that targets the hilA. 122 of 126 …
In Vitro Cultures For Production Of Zerumbone And Mass Propagation In Zingiber Zerumbet Smith., Farah Diana Idris
In Vitro Cultures For Production Of Zerumbone And Mass Propagation In Zingiber Zerumbet Smith., Farah Diana Idris
Student Works (2010-2019)
Lempoyang (Zingiber zerumbet Smith.) is an important plant which has been propagated because of its medicinal values. Lempoyang is widely used as a folk medicine in Asian countries such as Malaysia, Indonesia, India and Taiwan. Zerumbone, a cyclic sesqueterpene found to be the major and main bioactive compound that gives great potential in medicinal purposes especially in the treatment of chronic diseases based on recent reports. Recently, it was found that zerumbone inhibited the growth of human leukemia cell line, HL-60 cell. Even though there are so many great values contain in the lempoyang but it is hard to obtain …
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Student Works (2010-2019)
Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administration in rats, and to investigate the antibacterial activity of both extracts. Disc diffusion and broth dilution methods were carried out in determining the antibacterial effects of the plant extract on the pure cultures of clinical isolates of 8 MRSA and S. aureus ATCC strain. The disc-diffusion method of the ethanolic extracts showed inhibition zone …
Antagonistic Activity Of Selected Marine-Derived Streptomyces Spp. Against Plesiomonas Shigelloides., Angelyn Melaya Kloni
Antagonistic Activity Of Selected Marine-Derived Streptomyces Spp. Against Plesiomonas Shigelloides., Angelyn Melaya Kloni
Student Works (2010-2019)
In this study, primary and secondary screenings were conducted to evaluate the antibacterial and anti-yeast potential of the eleven selected marine-derived Streptomyces spp., particularly against P. shigelloides. The strains were categorized into three colour groups for morphological grouping according to the colour of aerial mycelium; namely grey, yellow green, and white colour groups. Diffusible pigments were produced by eight of the strains tested. Strain(s) from grey and white with red diffusible pigments colour groups displayed strong antibacterial activity. The yeasts tested were resistant to the bioactive metabolite(s) produced by some of the Streptomyces spp. which had shown high activity against …
Adsorption Of Lead (Ii) By A Non-Living Algal Biomass., Mudher A. Hussein
Adsorption Of Lead (Ii) By A Non-Living Algal Biomass., Mudher A. Hussein
Student Works (2010-2019)
Conventional techniques for removing dissolved heavy metals are only practical and cost-effective when applied to high strength wastes with heavy metal ion concentrations greater than 100 ppm. The possibility of using a non-living algal biomass to solve this problem was studied. Lead(II) was used in this study because it has been reported to cause several disorders in human. The non-living algal biomass was obtained from the filamentous green alga Spirogyra neglecta. The effects of initial concentration and contact time, pH, and temperature on the adsorption of lead(II) by the non-living algal biomass were studied. The equilibrium isotherms and kinetics were …
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Antibactertial Property And Toxcity Study Of Medicinal Plant Tinospora Crispa L., Altammar Khadijah Ahmed Y.
Student Works (2010-2019)
Tinospora crispa is a medically important herb used for centuries in traditional medicine for the treatment of various ailments. The aim of this study was to investigate the safety of the aqueous and alcoholic extract of Tinospora crispa by determining its toxicological effects after acute administration in rats, and to investigate the antibacterial activity of both extracts. Disc diffusion and broth dilution methods were carried out in determining the antibacterial effects of the plant extract on the pure cultures of clinical isolates of 8 MRSA and S. aureus ATCC strain. The disc-diffusion method of the ethanolic extracts showed inhibition zone …
A Study On Cell Suspension Cultures For Micropropagation And The Production Of Carpaine From Papaya (Carica Papaya L. Var. Eksotika)., Mohamad Fhaizal Mohamad Bukhori
A Study On Cell Suspension Cultures For Micropropagation And The Production Of Carpaine From Papaya (Carica Papaya L. Var. Eksotika)., Mohamad Fhaizal Mohamad Bukhori
Student Works (2010-2019)
Carica papaya L. is a member of Caricaceae family with four native genera. This fruit is economically important as in multi-purpose fruit produced for dessert and vegetable and is highly in demand for pharmaconomical trade particularly the plant compound as source of medicine. This species is a popular fruit crop in the tropical and sub-tropical regions of Malaysia, Thailand, Indonesia and the Philippines. It is also one of the most widely grown fruits in Malaysia and is mainly cultivated in Johor, Perak and Selangor. The Eksotika papaya variety was introduced to the farmers in the late eighties and this resulted …