Biology and Biomimetic Materials Commons^™

Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh

Faculty Works

The formation of composite films of double-stranded DNA and cationic lipid molecules (octadecylamine, ODA) and the hybridization of complementary single-stranded DNA molecules in such composite films are demonstrated. The immobilization of DNA is accomplished by simple immersion of a thermally evaporated ODA film in the DNA solution at close to physiological pH. The entrapment of the DNA molecules in the cationic lipid film is dominated by attractive electrostatic interaction between the negatively charged phosphate backbone of the DNA molecules and the protonated amine molecules in the thermally evaporated film and has been quantified using quartz crystal microgravimetry (QCM). Fluorescence studies …

Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh

Faculty Works

Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …

Characteristics And Performance Of R413a As An Alternative Refrigerant To R12, Waleed Mahmoud El-Nahas

Archived Theses and Dissertations

No abstract provided.

Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh

Faculty Works

Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …

Computer Assisted Instruction Wood Identification Program, Dave Moyer

Graduate Research Papers

In the development of wood identification skills, certain factors are important. These include: mastery of both terminology and technique, repetitive practice experiences, and an individualized approach in both guidance and feedback to the learner. The use of the computer as a programmed instructional aid provides for an individualized approach to learning, simulated teacher-student interaction, and feedback and reinforcement to stimulate learning. The problem of this activity is to develop a computer assisted instructional program to enable the learning of techniques for identifying selected samples of hardwoods.

Mechanical Testing Of Small, Clear Timber, Paul V. Mcclannahan

Graduate Research Papers

The purposes of the research project were:

1. To investigate the technological methods employed in mechanical testing of small clear timber.

2. To discover the uses, importance, and need for mechanical testing within the wood industry.

3. To experiment with the construction and development of various types of testing equipment similar to that being used by industry.

4. To explore the possibility of adapting mechanical testing of wood samples to the junior or senior high industrial arts wood laboratory

Wood Flour Molding, Charles Reed Snyder

Graduate Research Papers

The purposes of this research are briefly stated in the following:

1. Develop an understanding of the values of an industrial process through the use of raw materials and laboratory equipment.

2. Investigate wood flour molding and its relation to industry.

3. Experiment with materials, laboratory equipment, and wood flour mixtures to produce a molded article.

4. Develop an instructional unit for use in an industrial arts curriculum.

Jigs And Fixtures In Wood Industries, Arno K. Grabe

Graduate Research Papers

As an integral part of most woodworking industries, jigs and fixtures are worthy of some study. There is very little evidence of written material covering the topic even though the use of many of these devices is widespread throughout industry. The fact that these devices normally result in a substantial saving in production costs can often make them an important facet of a firm's business.

This saving can be accomplished in a number of different ways: use of unskilled workers, standardization of parts, interchangeability of pieces, reduction of safety hazards, and lowering of production time. Not all points will apply …

The Long Term Effects Of Frequent Cropping, Stanley Thomas Smith

Journal of the Department of Agriculture, Western Australia, Series 4

AGRICULTURALLY speaking, few Western Australian soils are more than 50 years old. We have the responsibility of preserving them for farming—not for one man's lifetime, but for centuries.

The aim of this article is to demonstrate that cropping and cultural practices do have some effects on the soil, and that these effects should be considered when choosing a farm rotation.