Digital Commons Network^™

Antiangiogenic Drugs In Combination With Seaweed Fucoidan: A Mechanistic In Vitro And In Vivo Study Exploring The Vegf Receptor And Its Downstream Signaling Molecules In Hepatic Cancer, Maha R.A. Abdollah, Aya A. Ali, Hassnaa Hamed Elgohary, M. M. Elmazar

Pharmacy

Hepatocellular carcinoma (HCC) is one of the most common cancers reported worldwide with poor morbidity and high mortality rates. HCC is a very vascular solid tumour as angiogenesis is not only a key driver for tumour progression but also an exciting therapeutic target. Our research investigated the use of fucoidan, a sulfated polysaccharide readily abundant in edible seaweeds commonly consumed in Asian diet due to their extensive health benefits. Fucoidan was reported to possess a strong anti-cancer activity, but its anti-angiogenic potential is still to be fully unraveled. Our research investigated fucoidan in combination with sorafenib (an anti-VEGFR tyrosine kinase …

2022-2023 Performance Forum - February 17, 2023, Jonathan Dills, Sheng Yuan Kuan, Kari Jenks, Juan Diaz Quijano, Silvia Valdivia Villasante, Aneliya Novikova

Performance Forum

No abstract provided.

Peter Pan Program, A-State Department Of Theatre

Stage Production Programs

This inventive adaptation completely reimagines the origin story of one of the world's most beloved children's books.

Azimuthal Correlations In Z +Jets Events In Proton–Proton Collisions At √S = 13tev, Cms Collaboration

Department of Physics and Astronomy: Faculty Publications

The production of Z bosons associated with jets is measured in pp collisions at √ s = 13 TeV with data recorded with the CMS experiment at the LHC corresponding to an integrated luminosity of 36.3 fb⁻¹. The multiplicity of jets with transverse momentum p_T > 30 GeV is measured for different regions of the Z boson’s p_T(Z ), from lower than 10 GeV to higher than 100 GeV. The azimuthal correlation Δφ between the Z boson and the leading jet, as well as the correlations between the two leading jets are measured in three regions …

An Older Diabetes-Induced Mice Model For Studying Skin Wound Healing, Carlos Poblete Jara, Guilherme Nogueira, Joseane Morari, Thaís Paulino Do Prado, Renan De Medeiros Bezerra, Lício A. Velloso, William Velander, Eliana Pereira De Araújo

Department of Chemical and Biomolecular Engineering: Faculty Publications

Advances in wound treatment depend on the availability of animal models that reflect key aspects of human wound healing physiology. To this date, the accepted mouse models do not reflect defects in the healing process for chronic wounds that are associated with type two diabetic skin ulcers. The long term, systemic physiologic stress that occurs in middle aged or older Type 2 diabetes patients is difficult to simulate in preclinical animal model. We have strived to incorporate the essential elements of this stress in a manageable mouse model: long term metabolic stress from obesity to include the effects of middle …

The George-Anne Inkwell Edition, Georgia Southern University

The Inkwell

No abstract provided.

Surveillance, State Secrets, And The Future Of Constitutional Rights, Laura K. Donohue

Georgetown Law Faculty Publications and Other Works

The Supreme Court’s 2022 decision in Federal Bureau of Investigation v. Fazaga heralds a worrying trend. Over the past 15 years, as more information about how the government wields its foreign intelligence collection authorities on U.S. soil has become available, it has become clear that the government has repeatedly acted outside its constitutional and statutory limits, and at times, in flagrant disregard for judicial orders. As a result, dozens of cases challenging surveillance have been making their way through the courts. Unlike in prior eras, in certain cases it has become easier for litigants to establish an injury-in-fact in light …

Harrisburg Order Confirming Joint Amended Plan Of Reorganization

Catholic Dioceses in Bankruptcy

No abstract provided.

Order Confirming Joint Amended Plan Of Reorganization

Catholic Dioceses in Bankruptcy

No abstract provided.

Welcome Home: A Policy Briefing Book For Housing Opportunity In St. Louis County, Molly W. Metzger

Center for Social Development Research

“Equality of opportunity” is a beautiful American ideal, but it stands in stark relief to the history of racist policies in the United States—including policies in the St. Louis region. Edited by Molly Metzger, this briefing book aims to broaden opportunity by offering ideas for housing policy change in the majority-white, inner-ring suburbs of St. Louis. The policy works in this collection were written by masters of social work students at Washington University’s Brown School as part of a social-policy course taught by Metzger in partnership with Mayor Laura Arnold of Webster Groves, Missouri, Webster Groves’ Alliance for Interracial Dignity, …

Linkage Fees: A Vehicle For Financing Affordable Housing, Lillian Murphy, Stanford Cooper, Russell Beckham

Center for Social Development Research

A linkage fee is a policy instrument for capturing resources to fund affordable housing development and other public services. This brief describes the instrument and offers recommendations on implementing linkage-fee policies.

The contents of this brief evolved from a presentation given in the Colloquium on Inclusive Housing Policy for the St. Louis Region. Held in the Brown School at Washington University on December 15, 2022, the event was organized by the Center for Social Development in partnership with Women’s Voices Raised for Social Justice and the Alliance for Interracial Dignity. The contents may also be found in Welcome Home: A …

Advancing Racial Equity Through Community Land Trusts, Amal Alzhrani, Brittany Kiefer, Da-Yup Kim, Gabby Eissner

Center for Social Development Research

Community land trusts are nonprofit organizations designed to preserve housing affordability. This brief describes the design and operation of such trusts, illustrating the concept by spotlighting the Columbia Community Land Trust of Columbia, Missouri.

The contents of this brief evolved from a presentation given in the Colloquium on Inclusive Housing Policy for the St. Louis Region. Held in the Brown School at Washington University on December 15, 2022, the event was organized by the Center for Social Development in partnership with Women’s Voices Raised for Social Justice and the Alliance for Interracial Dignity. The contents may also be found in …

Corrigendum: Strengthened Multi-Stakeholder Linkages In Valuation Studies Is Critical For Improved Decision Making Outcomes For Valuable Mangroves – The Malaysian Case Study, Soon Loong Lee, Amy Yee Hui Then, Hong Ching Goh, Caroline Hattam, Andrew Edwards-Jones, Melanie C. Austen

School of Biological and Marine Sciences

No abstract provided.

Drug-Like Small Molecules That Inhibit Expression Of The Oncogenic Microrna-21, Matthew D Shortridge, Bhawna Chaubey, Huanyu J Zhang, Thomas Pavelitz, Venkata Vidadala, Gregory L Olsen, Gabriele Varani

Faculty, Staff and Student Publications

We report the discovery of drug-like small molecules that bind specifically to the precursor of the oncogenic and pro-inflammatory microRNA-21 with mid-nanomolar affinity. The small molecules target a local structure at the Dicer cleavage site and induce distinctive structural changes in the RNA, which correlate with specific inhibition of miRNA processing. Structurally conservative single nucleotide substitutions eliminate the conformational change induced by the small molecules, which is also not observed in other miRNA precursors. The most potent of these compounds reduces cellular proliferation and miR-21 levels in cancer cell lines without inhibiting kinases or classical receptors, while closely related compounds …

Adjuvant Chemotherapy Versus Adjuvant Concurrent Chemoradiotherapy After Radical Surgery For Early-Stage Cervical Cancer: A Randomized, Non-Inferiority, Multicenter Trial, Danhui Weng, Huihua Xiong, Changkun Zhu, Xiaoyun Wan, Yaxia Chen, Youzhong Zhang, Xi Zhang, Qinglei Gao, Gang Chen, Hui Xing, Changyu Wang, Kezhen Li, Yaheng Chen, Yuyan Mao, Dongxiao Hu, Zimin Pan, Qingqin Chen, Baoxia Cui, Kun Song, Cunjian Yi, Guangcai Peng, Xiaobing Han, Ruifang An, Liangsheng Fan, Wei Wang, Tingchuan Xiong, Yile Chen, Zhenzi Tang, Lin Li, Xingsheng Yang, Xiaodong Cheng, Weiguo Lu, Hui Wang, Beihua Kong, Xing Xie, Ding Ma

Faculty, Staff and Student Publications

We conducted a prospective study to assess the non-inferiority of adjuvant chemotherapy alone versus adjuvant concurrent chemoradiotherapy (CCRT) as an alternative strategy for patients with early-stage (FIGO 2009 stage IB-IIA) cervical cancer having risk factors after surgery. The condition was assessed in terms of prognosis, adverse effects, and quality of life. This randomized trial involved nine centers across China. Eligible patients were randomized to receive adjuvant chemotherapy or CCRT after surgery. The primary end-point was progression-free survival (PFS). From December 2012 to December 2014, 337 patients were subjected to randomization. Final analysis included 329 patients, including 165 in the adjuvant …

Exploiting Prmt5 As A Target For Combination Therapy In Mantle Cell Lymphoma Characterized By Frequent Atm And Tp53 Mutations, Yuxuan Che, Yang Liu, Yixin Yao, Holly A Hill, Yijing Li, Fangfang Yan, Wei Wang, Lixin Rui, Michael Wang

Faculty, Staff and Student Publications

Constant challenges for the treatment of mantle cell lymphoma (MCL) remain to be recurrent relapses and therapy resistance, especially in patients harboring somatic mutations in the tumor suppressors ATM and TP53, which are accumulated as therapy resistance emerges and the disease progresses, consistent with our OncoPrint results that ATM and TP53 alterations were most frequent in relapsed/refractory (R/R) MCL. We demonstrated that protein arginine methyltransferase-5 (PRMT5) was upregulated in R/R MCL, which predicted a poor prognosis. PRMT5 inhibitors displayed profound antitumor effects in the mouse models of MCL with mutated ATM and/or TP53, or refractory to CD19-targeted CAR T-cell therapy. …

Cd5 Expression By Dendritic Cells Directs T Cell Immunity And Sustains Immunotherapy Responses, Mingyu He, Kate Roussak, Feiyang Ma, Nicholas Borcherding, Vince Garin, Charles Schutt, Yun Zhao, Courtney A Iberg, Kairav Shah, Himanshi Bhatia, Daniel Korenfeld, Sabrina Dinkel, Judah Gray, Alina Ulezko Antonova, Stephen Ferris, David Donermeyer, Cecilia Lindestam Arlehamn, Matthew M Gubin, Jingqin Luo, Laurent Gorvel, Matteo Pellegrini, Alessandro Sette, Thomas Tung, Rasmus Bak, Robert L Modlin, Ryan C Fields, Robert D Schreiber, Paul M Allen, Eynav Klechevsky

Faculty, Staff and Student Publications

The induction of proinflammatory T cells by dendritic cell (DC) subtypes is critical for antitumor responses and effective immune checkpoint blockade (ICB) therapy. Here, we show that human CD1c+CD5+ DCs are reduced in melanoma-affected lymph nodes, with CD5 expression on DCs correlating with patient survival. Activating CD5 on DCs enhanced T cell priming and improved survival after ICB therapy. CD5+ DC numbers increased during ICB therapy, and low interleukin-6 (IL-6) concentrations promoted their de novo differentiation. Mechanistically, CD5 expression by DCs was required to generate optimally protective CD5hi T helper and CD8+ T cells; further, deletion of CD5 from T …

Endothelial-To-Osteoblast Transition In Normal Mouse Bone Development, Xingzhi Song, Yoshihiro Komatsu, Li-Yuan Yu-Lee, Guocan Wang, Sue-Hwa Lin

Faculty, Staff and Student Publications

Metastatic prostate cancer (PCa) in bone induces bone-forming lesions. We have previously shown that PCa-induced bone originates from endothelial cells (ECs) that have undergone EC-to-osteoblast (OSB) transition. Here, we investigated whether EC-to-OSB transition also occurs during normal bone formation. We developed an EC and OSB dual-color reporter mouse (DRM) model that marks EC-OSB hybrid cells with red and green fluorescent proteins. We observed EC-to-OSB transition (RFP and GFP co-expression) in both endochondral and intramembranous bone formation during embryonic development and in adults. Co-expression was confirmed in cells isolated from DRM. Bone marrow– and lung-derived ECs underwent transition to OSBs and …

Exploiting Metabolic Vulnerabilities After Anti-Vegf Antibody Therapy In Ovarian Cancer, Deanna Glassman, Mark S Kim, Meredith Spradlin, Sunil Badal, Mana Taki, Prasanta Dutta, Katherine I Foster, Olamide Animasahun, Jin Heon Jeon, Abhinav Achreja, Anusha Jayaraman, Praveen Kumar, Minal Nenwani, Fulei Wuchu, Emine Bayraktar, Yutuan Wu, Elaine Stur, Lingegowda Mangala, Sanghoon Lee, Timothy A Yap, Shannon N Westin, Livia S Eberlin, Deepak Nagrath, Anil K Sood

Faculty, Staff and Student Publications

Despite modest clinical improvement with anti-vascular endothelial growth factor antibody (AVA) therapy in ovarian cancer, adaptive resistance is ubiquitous and additional options are limited. A dependence on glutamine metabolism, via the enzyme glutaminase (GLS), is a known mechanism of adaptive resistance and we aimed to investigate the utility of a GLS inhibitor (GLSi). Our in vitro findings demonstrated increased glutamine abundance and a significant cytotoxic effect in AVA-resistant tumors when GLSi was administered in combination with bevacizumab. In vivo, GLSi led to a reduction in tumor growth as monotherapy and when combined with AVA. Furthermore, GLSi initiated after the emergence …

Batf2 Promotes Hsc Myeloid Differentiation By Amplifying Ifn Response Mediators During Chronic Infection, Brandon T Tran, Bailee Kain, Yingmin Zhu, Kurt Christensen, Antrix Jain, Anna Malovannaya, Katherine Y King

Faculty, Staff and Students Publications

Basic leucine zipper ATF-like transcription factor 2 (BATF2), an interferon-activated immune response regulator, is a key factor responsible for myeloid differentiation and depletion of HSC during chronic infection. To delineate the mechanism of BATF2 function in HSCs, we assessed Batf2 KO mice during chronic infection and found that they produced less pro-inflammatory cytokines, less immune cell recruitment to the spleen, and impaired myeloid differentiation with better preservation of HSC capacity compared to WT. Co-IP analysis revealed that BATF2 forms a complex with JUN to amplify pro-inflammatory signaling pathways including CCL5 during infection. Blockade of CCL5 receptors phenocopied Batf2 KO differentiation …

Transcriptome, Proteome, And Protein Synthesis Within The Intracellular Cytomatrix, Amanda Williams, Abdol-Hossein Rezaeian, Daniel Kraushaar, Emily Ricco, Matthew J Robertson, Cristian Coarfa, Antrix Jain, Anna Malovannaya, Fabio Stossi, Antone R Opekun, Alyssa P Price, Julien Dubrulle

Faculty, Staff and Students Publications

Despite the knowledge that protein translation and various metabolic reactions that create and sustain cellular life occur in the cytoplasm, the structural organization within the cytoplasm remains unclear. Recent models indicate that cytoplasm contains viscous fluid and elastic solid phases. We separated these viscous fluid and solid elastic compartments, which we call the cytosol and cytomatrix, respectively. The distinctive composition of the cytomatrix included structural proteins, ribosomes, and metabolome enzymes. High-throughput analysis revealed unique biosynthetic pathways within the cytomatrix. Enrichment of biosynthetic pathways in the cytomatrix indicated the presence of immobilized biocatalysis. Enzymatic immobilization and segregation can surmount spatial impediments, …

Deepbend: An Interpretable Model Of Dna Bendability, Md Abul Hassan Samee

Faculty, Staff and Students Publications

The bendability of genomic DNA impacts chromatin packaging and protein-DNA binding. However, we do not have a comprehensive understanding of the motifs influencing DNA bendability. Recent high-throughput technologies such as Loop-Seq offer an opportunity to address this gap but the lack of accurate and interpretable machine learning models still remains. Here we introduce DeepBend, a convolutional neural network model with convolutions designed to directly capture the motifs underlying DNA bendability and their periodic occurrences or relative arrangements that modulate bendability. DeepBend consistently performs on par with alternative models while giving an extra edge through mechanistic interpretations. Besides confirming the known …

The Gut Microbiota Of People With Asthma Influences Lung Inflammation In Gnotobiotic Mice, Naomi G Wilson, Ariel Hernandez-Leyva, Anne L Rosen, Natalia Jaeger, Ryan T Mcdonough, Jesus Santiago-Borges, Michael A Lint, Thomas R Rosen, Christopher P Tomera, Leonard B Bacharier, S Joshua Swamidass, Andrew L Kau

2020-Current year OA Pubs

The gut microbiota in early childhood is linked to asthma risk, but may continue to affect older patients with asthma. Here, we profile the gut microbiota of 38 children (19 asthma, median age 8) and 57 adults (17 asthma, median age 28) by 16S rRNA sequencing and find individuals with asthma harbored compositional differences from healthy controls in both adults and children. We develop a model to aid the design of mechanistic experiments in gnotobiotic mice and show enterotoxigenic

The Use Of Characteristics And Indicators Of Body Construction As Predictors In The Identification Of The Angle Values Of The Physiological Curves Of The Spine In Sequential Objective Testing - Mathematical Models, Jacek Tuz, Adam Maszczyk, Anna Zwierzchowska

Baltic Journal of Health and Physical Activity

Introduction: The physiological curvatures of the spine in the sagittal plane are constantly evolving along with changes in the structure and proportions of the body and physical activity. The aim of this study is to identify and assess the strength of the influence of body features and indices on the values of lumbar lordosis and thoracic kyphosis angles with the use of a mathematical model. In the years 2006–2016, 1,314 female students aged ( ± SD 19.7 ± 0.4) (min.–max. 18.1–22.5) were examined.

Materials and Methods. The following measurements were made: body mass (BM), body height (BH), waist circumference (WC), …

Rediscovering The Artistic Side Of Mathematics, Bogdan G. Nita, Ashwin Vaidya

LASER Journal

Welcome to the inaugural issue of the LASER, a journal devoted to the problems at the interface of math and art. The terms ’math’ and ’art’ are to be broadly construed to encompass all quantitative sciences and forms of art. The journal’s name, acronym for Linking Art and Science through Education and Research, suggests our interest in the theory, practice and pedagogy of this interdisciplinary subject.

How Does Bpm Maturity Affect Process Performance?

Communications of the IIMA

Organizations are increasingly confronted with the consequences of globalization, digitalization and disruptive (technological) developments. The constantly changing and dynamic social, economic, and political environment as well as the public opinion require public organizations to deliver effective and efficient services. These trends also apply to Dutch housing associations who are working together with suppliers in the process of property maintenance to increase quality and to reduce costs. Business Process Management (BPM) has the prospect of enhancing the performance of maintenance processes. Previous studies have confirmed a positive relationship between BPM-maturity and Process performance. However, in different contexts this relation is sometimes …

Cyber Threat Intelligence Discovery Using Machine Learning From The Dark Web

Communications of the IIMA

Cyber threat intelligence (CTI) is an actionable information or insight an organization uses to understand potential vulnerabilities it does have and threats it is facing. One important CTI for proactive cyber defense is exploit type with possible values system, web, network, website or Mobile. This study compares the performance of machine learning algorithms in predicating exploit types using form posts in the dark web, which is a semi- structured dataset collected from dark web. The study uses the CRISP data science approach. The results of the study show that machine learning algorithms which are function-based including support vector machine and …

Yield And Composition Of Forage Maize: Interaction Of Harvest Date, Cultivar And Plastic Mulch, E. M. Little, P. O'Kiely, J. C. Crowley, G. P. Keane

IGC Proceedings (1977-2023)

Forage maize is established as a crop with the potential to consistently supply high yields of quality forage on some Irish farms. Despite its success, considerable variability in crop yield, quality and maturity at harvest can exist from year to year. These reflect differing prevailing weather conditions, particularly temperature during May to September. The use of plastic mulch has increased the likelihood of achieving higher yields of high quality crops and has permitted maize production to extend into areas once considered unsuitable for the crop. In this experiment two cultivars of differing maturity were grown with or without plastic mulch …

Fermentation Characteristics Of Maize/Sesbania Bi-Crop Silage, M. Kondo, J. Yanagisawa, K. Kita, H. Yokota

IGC Proceedings (1977-2023)

Maize is one of the main forages for dairy production and is a suitable material for silage making because of high fermentable carbohydrates, high counts of lactic acid bacteria (LAB) and low buffering capacity (BC) (Nishino et al. 2003; McDonald et al. 1991). Whole crop maize silage is high in energy but low in crude protein (CP). On the other hand, legumes are high in CP but difficult to conserve because of their low water soluble carbohydrates (WSC) and high BC. It might be possible that maize/legume bi-crop silage compensate for their negative points. Sesbania is a legume originated …

Ensiling Of Tannin-Containing Sorghum Grain, E. M. Ott, Y. Acosta Aragón, M. Gabel

IGC Proceedings (1977-2023)

Sorghum is known as important feed-stuff in tropical regions where rainfall is insufficient for the cultivation of maize. Furthermore, those sorghum cultivars rich in tannins are naturally protected to a certain extent against bird damage, insect pests and moulds. Nevertheless, tannins impair the feed quality. Thus, the objectives of this study were to investigate whether ensiling could be a suitable preservation method for sorghum grain originally rich in tannins and if it is possible to reduce tannin content during fermentation.