Digital Commons Network^™

Designing A Tpack-Based Mobile Learning Model For Biology Education Using Nominal Group Techniques, Cik Jamaliah Abd Manaf, Shakinaz Desa, Muhammad Ikhwan Mat Saad, Helen Crompton

STEMPS Faculty Publications

Mobile learning (m-learning) enhances STEM education, particularly in biology, by supporting visualization and interaction. However, a structured framework integrating technological, pedagogical, and content knowledge (TPACK) is essential. This study designs a TPACK-based mobile learning model for biology using the Nominal Group Technique (NGT). A workshop with 10 experts identified seven key components and 67 elements, with nine elements rejected due to low consensus. The validated framework will guide model development using Interpretive Structural Modelling (ISM) to enhance critical thinking, collaboration, and digital citizenship, ultimately fostering effective mobile learning environments for biology education.

Examining Teaching Competencies And Challenges While Integrating Artificial Intelligence In Higher Education, Xinyue Ren, Min Lun Wu

STEMPS Faculty Publications

The rapid development of artificial intelligence (AI) technologies has demonstrated their affordances and limitations in revolutionizing pedagogical strategies in higher education. Given the lack of guidelines, policies, and resources to assist instructors in efficiently and ethically integrating AI into teaching and learning practices, this systematic review aimed to investigate AI integration competencies and challenges in higher education from the intelligent Technological Pedagogical Content Knowledge (TPACK) perspective. We first applied the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) to identify 23 studies published between 2019 and 2023 that met the inclusion and exclusion criteria. After conducting open coding and …

Advancing Pedagogical And Instructional Design Through Artificial Intelligence (Ai) In Education And Training Contexts, Mohan Yang, Jewoong Moon, Tian Luo, Jinhee Kim

STEMPS Faculty Publications

[Introduction] "The only way to discover the limits of the possible is to go beyond them into the impossible." - Arthur C. Clarke

It is our pleasure and honor as guest editors for this special issue of the Journal of Applied Instructional Design (JAID) to present "Advancing Pedagogical and Instructional Design through Artificial Intelligence (AI) in Education and Training Contexts." This issue arrives at a truly timely moment, as the rapid and continuous development of generative artificial intelligence (GenAI) is fundamentally reshaping the traditional paradigms of teaching, learning, and training.

Culture Wars In The Commonwealth: Public Libraries, Book Bans, And The First Amendment, Natisha Harper, Amber Matthews

STEMPS Faculty Publications

Public libraries are quickly losing the ‘culture war’ being waged against them (Jones, 2024). They also lack the coordinated support, resources, and legislative power of those leading the national assault on the right to read, intellectual freedom, and the First Amendment (ALA, 2024). Few resources provide timely and relevant guidance for public libraries on negotiating book challenges with concerned community members, elected officials, and interested parties. This research study responds to nascent ‘culture war’ developments in Virginia – specifically, recent government interference in public library governance in Warren County in December (Schneider, 2024) and a new bill targeting ‘obscene’ materials …

Disorienting "Western" Viewers With Nacer Khemir's Desert Trilogy, Kristian Peterson

Philosophy Faculty Publications

In this essay, I explore tensions that arise when creating alternative cinematic visions of Muslim life, specifically frictions emerging from the goal of shifting public perception of the community and patterns of self-orientalization. Here, I complicate assumptions about the representation of Islam by looking at a self-articulated presentation of Muslims through the narrative feature series the Desert Trilogy, by Tunisian director Nacer Khemir (born 1948). I argue that Khemir is ensnared by ahistorical Islamic nostalgia and largely reinforces romantic orientalist aesthetics in his films. While his portrait of Muslims is much more “positive” than those in the broader archive, it …

Sex-Dependent Changes In Risk-Taking Predisposition Of Rats Following Space Radiation Exposure, Elliot Smits, Faith E. Reid, Ella N. Tamgue, Paola Alvarado Arriaga, Charles Nguyen, Richard A. Britten

Department Radiation Oncology & Biophysics Faculty Publications

The Artemis missions will establish a sustainable human presence on the Moon, serving as a crucial steppingstone for future Mars exploration. Astronauts on these ambitious missions will have to successfully complete complex tasks, which will frequently involve rapid and effective decision making under unfamiliar or high-pressure conditions. Exposure to low doses of space radiation (SR) can impair key executive functions critical to decision making. This study examined the effects of exposure to 10 cGy of Galactic Cosmic Ray simulated radiation (GCRsim) on decision-making performance in male and female rats with a naturally low predisposition for risk-taking (RTP) prior to exposure. …

A Two-Hit Model Of Executive Dysfunction: Simulated Galactic Cosmic Radiation Primes Latent Deficits Revealed By Sleep Fragmentation, Richard A. Britten, Ella N. Tamgue, Paola Arriaga Alvarado, Arriyam S. Fesshaye, Larry D. Sanford

Department Radiation Oncology & Biophysics Faculty Publications

Future Artemis-class missions to Mars will expose astronauts to prolonged space radiation (SR), sleep disruption, and operational demands requiring greater autonomy, placing decision making and executive function at heightened risk. Both SR and sleep fragmentation (SF) independently impair cognition, yet their combined effects remain poorly understood. Using the Associative Recognition Memory and Interference (ARMIT) task, we assessed cognitive performance in male rats exposed to 10 cGy of Galactic Cosmic Ray simulation (GCRsim), SF, or both. Under well-rested conditions, GCRsim-exposed rats exhibited overt deficits in the C.1.2 stage, performing at chance when reinforcement contingencies shifted, consistent with impaired cognitive flexibility. In …

Cluster Conversation: (Re)Writing Our Histories, (Re)Building Feminist Worlds: Working Toward Hope In The Archives, Ruth Osorio, Lamaya Williams, Megan Mcintyre

English Faculty Publications

[Introduction] "Hope is not like a lottery ticket you can sit on the sofa and clutch, feeling lucky. [...] Hope is an ax you break down doors with in an emergency." - Rebecca Solnit

In 2018, Cheryl Glenn wrote, "The work of feminist rhetorical historiography is far from done; in fact, it has just begun-and it is anchored in hope." Following Glenn, we explore hope in this cluster as a methodological imperative in the archives. Informed by theorists Paulo Freire, bell hooks, Rebecca Solnit, and Cornel West, the writers in this Cluster Conversation envision hope as a radical orientation toward …

Threats To Democracy: A Danger To Social Justice For All, José Luis Irizarry, Rajade M. Berry-James, Duchess D. Humphrey, Rachel Emas, Bruce D. Mcdonald Iii, James Nordin, Michael Lee, Lindsey L. Evans, Anthony M. Starke Jr., Jay M. Grosflam

School of Public Service Faculty Publications

This essay argues that threats to democracy—both global and domestic—pose an existential risk to the advancement of social justice. It explores how democratic backsliding undermines efforts to achieve social justice for all, examining anti-democratic practices such as misinformation, voter suppression, institutional dismantling, and the politicization of public service. Highlighting the interdependence between democratic institutions and social equity in public governance, the authors propose a multilevel response framework to foster democratic resilience through civic engagement, institutional accountability, and collective action. In this essay, members of the Board of the Section on Democracy and Social Justice (DSJ) of the American Society for …

Atmospheric Chemistry Experiment (Ace) Winds, Matthew Wyatt, Peter F. Bernath, Chris Boone, Léo Lavy, Ryan Johnson

Chemistry & Biochemistry Faculty Publications

The Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS) uses limb geometry to measure transmittance spectra of Earth's atmosphere by solar occultation. Line-of-sight wind speeds can be derived via Doppler shifts of molecular lines in infrared spectra. The wind look direction angles relative to geodetic North are derived from geometry. We validate the new ACE version 5.3 (v.5.3) line-of-sight winds with MIGHTI and meteor radar vector wind observations and find a ±15 m/s sunrise/sunset shift above 80 km. We also compare line-of-sight winds from ACE-FTS v.5.2 and v.5.3 with vector winds from the MERRA-2, HWM14, and WACCM-X models. A ±15 m/s …

Integrating Phytochemicals And In Silico Methods For Modern Drug Discovery: A Comprehensive Review, Olorunfemi Oyewole Babalola, Kpomah Bridget, Godspower Oyubu, Sakariyau Adio Waheed, Samuel Ayomikun Ajiboye, Aderonke Elizabeth Fakayode, Adeleye Adegboyega Edema, Udensi Great Chukwuma, Rachael Taiwo Tiamiyu, Adebisola Joy Fakayode, Hameedah Oluwatoyin Adebimpe, Kosisochukwu Dematus Onyeagba, Comfort Oluseun Fakunle, Paul Olamide Ottu, Sonia Gabriel

Chemistry & Biochemistry Faculty Publications

Phytochemicals have long played a central role in drug discovery due to their structural diversity and broad biological activity. This review explores the progression of natural product-based drug development, highlighting how traditional knowledge and modern scientific tools are increasingly being combined. Medicinal plants identified through ethnopharmacological practices continue to guide the search for bioactive compounds, while advancements in screening techniques and structural analysis have improved the isolation and characterization of these substances. The review discusses the wide range of pharmacological actions found in plant-derived compounds. Their mechanisms of action and potential for synergistic effects are examined in detail. The application …

Phytochemical And Nutritional Composition Of Vernonia Amygdalina: Antioxidant, Antidiabetic Efficacy, And Hematological Benefits, Hassan A. Adewuyi, Messiah Atapia, Emmanuel Ochonung, Sakariyau A. Waheed, Adebimpe H. Oluwatoyin, Chizoba V. Obunadike, Adeleye A. Edema, Samad Hussein, Usman A. Mohammed, Kevin L. Sebastine, Tolulope O. Jaiyeola

Chemistry & Biochemistry Faculty Publications

Vernonia amygdalina, a traditionally used medicinal plant, has been purported to possess health-promoting properties. The aim of this study is to investigate the phytochemical and nutritional composition of V. amygdalina, evaluate its antioxidant and antidiabetic efficacy, and assess its hematological benefits. Phytochemical screening, proximate analysis, antioxidant assays (DPPH, ABTS), antidiabetic evaluations in treptozotocin induced diabetic rats, and hematological assessments were conducted according to standard protocol. V. amygdalina exhibited a rich phytochemical profile, comprising flavonoids, phenolic acids, and terpenoids, alongside significant antioxidant activity (IC50 = 12.5 μg/mL) and complete source of protein. Proximate analysis revealed high protein (21.1%) and …

Evaluating The Chemical Reactivity Of Wildfire-Derived Dissolved Organic Molecules: Glutathione Binding Through Kendrick Mass Defect Analysis, Hannah M. Hamontree, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The emerging risks to organisms of pyrogenic-derived dissolved organic matter (PyDOM) from forest fires are of concern due to its toxic and mutagenic potential (e.g., pro-oxidative responses in fauna through the depletion of glutathione, a nitrogen- and sulfur-containing tripeptide found in cells). This study simulates this phenomenon in a laboratory setting by identifying bonding between reduced l-glutathione and organic molecules in leachates from environmentally weathered biomass samples (charred and uncharred) using Kendrick Mass Defect (KMD) analysis from formula lists obtained from negative-mode electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry ((-)ESI-FT-ICR-MS). These formula lists reveal a 10-fold increase in nitrogen- and sulfur-containing …

The Importance Of Atomic Charges For Predicting Site-Selective Ir-, Ru-, And Rh-Catalyzed C-H Borylations, Shannon M. Stephens, Kyle M. Lambert

Chemistry & Biochemistry Faculty Publications

A supervised machine learning model has been developed that allows for the prediction of site selectivity in late-stage C-H borylations. Model development was accomplished using literature data for the site-selective (≥95%) C-H borylation of 189 unique arene, heteroarene, and aliphatic substrates that feature a total of 971 possible sp² or sp³ C-H borylation sites. The reported experimental data was supplemented with additional chemoinformatic descriptors, computed atomic charges at the C-H borylation sites, and data from parameterization of catalytically active tris-boryl complexes resulting from the combination of seven different Ir-, Ru-, and Rh-based precatalysts with eight different ligands. Of the over …

The Role Of Social Isolation In Shaping Health Outcomes In Older Adults: A Regional Analysis, Vince Falco, Lynn Wiles, Jonathan Romero, Leorey Saligan

Ellmer School of Nursing Faculty Publications

Social isolation is a critical public health concern in older adults and is linked to a range of adverse health outcomes across diverse sociodemographic groups. This study investigated the associations between social isolation with physical and mental health of older adults residing in Hampton Roads, a racially diverse region in Southeastern Virginia, home to a large population of senior military veterans. Secondarily, the study explored how social determinants of health (SDoH) and comorbid medical conditions influenced the relationship of social isolation with physical and mental health of these seniors. Physical and mental health were measured using the Patient-Reported Outcomes Measurement …

Unlocking The Potential Of Primary Healthcare Nurse Practitioners Globally: A Concept Analysis Of Their Added Value, Isabelle Savard, Gina Sophia Costanzo, Colette Henderson, Deborah C. Gray, Melanie Rogers, Kelley Kilpatrick

Ellmer School of Nursing Faculty Publications

Background: Primary healthcare nurse practitioners (PHCNPs) are expanding globally to meet rising healthcare demands. Despite positive outcomes, their added value remains underexplored.

Purpose: To clarify and refine the added value PHCNPs bring.

Methods: Building on a previous concept analysis of the added value of nurse practitioners, we analyzed 37 systematic reviews focusing on PHCNPs’ roles across various settings, using Rodgers’ evolutionary approach.

Findings: Attributes include PHCNPs’ skills and competencies, activities, positive outcomes, and professional autonomy. Antecedents involve PHCNP characteristics and structural and institutional factors, while consequences highlight improved patient outcomes, satisfaction, professional dynamics, and system efficiency.

Discussion: Understanding PHCNPs’ added …

Challenges Associated With Pfas Detection Method In Africa, Abdullahi Tunde Aborode, Ridwan Olamilekan Adesola, Ibrahim Idris, Waheed Sakariyau Adio, Segun Olapade, Gladys Oluwafisayo, Israel Ayobami Onifade, Sodiq Fakorede, Taiwo Bakare-Abidola, Jelil Olaoye, Adedeji Daniel Ogunyemi, Oluwaseun Adeolu Ogundijo, Olamilekan Gabriel Banwo, Adetolase Azizat Bakre, Peter Oladoye, Grace Adegoye, Noimat Abeni Jinadu

Chemistry & Biochemistry Faculty Publications

Per- and polyfluoroalkyl substances (PFAS) are a group of man-made chemicals that are widely present in many industries. Monitoring and analyzing PFAS in Africa is challenging due to the limited availability of mass spectrometry (MS), which is an essential technique for detecting PFAS. This review assesses the scope and impact of the shortage of mass spectrometry instruments in Africa, emphasizing the resulting limitations in monitoring environmental and public health threats. The review analyzes the existing PFAS monitoring, the accessibility of MS instruments, and the technical capabilities within the continent. This study suggests that fewer African countries have sufficient MS instruments, …

Bacterial Two-Hybrid Systems Evolved: Innovations For Protein-Protein Interaction Research, Rebecca M. Richardson, Steven M. Pascal

Chemistry & Biochemistry Faculty Publications

Bacterial two-hybrid (B2H) systems offer a versatile platform for detecting protein-protein interactions (PPIs) in vivo. Originally developed to study bacterial transcriptional regulators, these systems have evolved to support a wide range of applications, including interaction mapping, domain analysis, and high-throughput screening. This review surveys the development and expansion of B2H systems across diverse biological contexts. We highlight technical considerations, platform-specific innovations, and recent adaptations that extend the utility of B2H systems in both prokaryotic and eukaryotic protein interaction research environments. By comparing different B2H variants and discussing their integration with complementary approaches, we provide a framework for leveraging these …

Clostridioides Difficile Infection Study Models And Prospectives For Probing The Microbe-Host Interface, Tatiana Zvonareva, David S. Courson, Erin B. Purcell

Chemistry & Biochemistry Faculty Publications

Clostridioides difficile infection (CDI) is an urgent public health threat with a high rate of recurrence and limited treatment options. In vivo models have been indispensable in understanding CDI pathophysiology and establishing treatment protocols and continue to be essential in pre-clinal testing. More importantly, in vivo models offer the opportunity to probe the complex systemic host response to the microbe, which is impossible to recapitulate in vitro. Nonetheless, constraints related to the availability of animal models, cost, ethical considerations, and regulatory control limit their accessibility for basic research. Furthermore, physiological and habitual divergences between animal models and humans often result …

Antihypertensive Potential Of Euphorbia Hirta And Leptadenia Hastata In Adrenaline-Induced Wistar Rats, Fagbohun Oyenike Bushirat, Emmanuel Ogar Ochonung, Messiah Innocent Atapia, Hassan Abdulsalam Adewuyi, Sakariyau Aido Waheed, Adebimpe Oluwatoyin Hameedah, Chizoba Victory Obunadike, Adeleye Adegboyega Edema, Samad Hussein, Abiola Mohammed Usman, Tolulope Olukayode Jaiyeola

Chemistry & Biochemistry Faculty Publications

Background: Hypertension is a major public health concern worldwide, contributing to cardiovascular morbidity and mortality. The use of plant-based therapies, such as Euphorbia hirta and Leptadenia hastata, has gained attention for their potential antioxidant, anti-hyperlipidemic, and organ-protective effects.

Objectives: This study aimed to investigate the phytotherapeutic approach to hypertension using E. hirta and L. hastata extracts in adrenaline-induced hypertensive Wistar rats

Methods: Utilizing an adrenaline-induced hypertensive rat model, we assessed the effects of E. hirta and L. hastata leaf extracts (50, 100, and 200 mg/kg) on systolic blood pressure, renal function, lipid metabolism, and hematological parameters.

Results: Antioxidant activity: …

Nucleotide-Derived Bacterial Alarmones Attenuate The Induction Of Type-I Interferon Responses In A Murine Macrophage Reporter Cell Line, Ryan P. Kilduff, Erin B. Purcell, Lisa M. Shollenberger

Chemistry & Biochemistry Faculty Publications

The stringent response is a well-studied phenomenon in many bacterial systems and regulates resource-consuming activities such as transcription, translation, and replication. The stringent response is a well-conserved signaling framework, as are the nucleotide-derived signaling mediators, collectively referred to as (p)ppGpp or as alarmones. There is a wealth of research evaluating nucleotide-derived alarmone signaling in bacterial models, however, their potential to modulate innate immune signaling has not yet been evaluated. Several common pathogen-synthesized molecules, such as lipopolysaccharide (LPS) and cyclic-di-AMP (c-di-AMP), act as pathogen-associated molecular patterns (PAMPs), which are common patterns that alert the innate immune system of bacterial infection. The …

Green Chelation Strategy For Deashing Of Algal Biomass, Agyare Asante, George Daramola, Ryan W. Davis, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

This study investigated a green chelation strategy for deashing algal biomass using nitrilotriacetic acid (NTA) and deionized water (DI) to enhance its suitability for biofuel and bioproduct applications. Solid-state algal turf scrubber (SS ATS), green algal turf scrubber (ATS), and Scenedesmus were analyzed, with Scenedesmus selected for detailed evaluation due to its high ash removal efficiency. The objective was to optimize a purification process that minimizes ash and heavy metal content while preserving biochemical integrity. Algal biomass underwent sequential washing with DI, NTA, and NTA+DI under varying temperatures (90-130 °C). Analytical techniques including Fourier Transform Infrared (FTIR) spectroscopy, Inductively Coupled …

Social Susceptibility-Driven Longitudinal Tornado Reconnaissance Methodology: 2021 Midwest Quad-State Tornado Outbreak, John W. Van De Lindt, Wanting "Lisa" Wang, Blythe Johnston, P. Shane Crawford, Guirong Yan, Thang Dao, Trung Do, Katie Skakel, Mojtaba Harati, Tu Nguyen, Robinson Umeike, Silvana Croope

Civil & Environmental Engineering Faculty Publications

With the impact of climate change, the intensity and frequency of tornado events have been increasing. Enhancing tornado reconnaissance methods can comprehensively capture building damage and recovery data following tornado events and outbreaks, thereby strengthening community resilience against the threat of future tornado events. Advancements in tornado data reconnaissance research have embraced remote sensing techniques to assess building damage after tornado events, supplanting traditional reconnaissance methods relying on handheld cameras with GIS mapping. Community resilience research offers a groundbreaking perspective, stressing the importance of assessing buildings throughout their recovery cycle-from damage and functionality to recovery-and considering their socioeconomic stability in …

Biochar For Soil Amendment: Applications, Benefits, And Environmental Impacts, Ujjwal Pokharel, Gururaj Neelgund, Ram L. Ray, Venkatesh Balan, Sandeep Kumar

Civil & Environmental Engineering Faculty Publications

The excessive use of chemical fertilizers results in environmental issues, including loss of soil fertility, eutrophication, increased soil acidity, alterations in soil characteristics, and disrupted plant–microbe symbiosis. Here, we synthesize recent studies available from up to 2025, focusing on engineered biochar and its application in addressing issues of soil nutrient imbalance, soil pollution from inorganic and organic pollutants, soil acidification, salinity, and greenhouse gas emissions from fields. Application of engineered biochar enhanced the removal of Cr (VI), Cd²⁺, Ni²⁺, Zn²⁺, Hg²⁺, and Eu³⁺ by 85%, 73%, 57.2%, 12.7%, 99.3%, and 99.2%, …

A Global Application Programming Interface-Enabled Earthquake Ground Motion Relational Database For Engineering Applications, Tristan E. Buckreis, Chukwuebuka C. Nweke, Pengfei Wang, Scott J. Brandenberg, Maria E. Ramos-Sepúlveda, Rashid Shams, Shako Mohammed, Renmin Pretell, Silvia Mazzoni, Paolo Zimmaro, Jonathan P. Steward

Civil & Environmental Engineering Faculty Publications

We present a application programming interface (API)-enabled relational database of global earthquake ground motion intensity measures, associated metadata, and processed time-series data. Raw ground motion records were processed by the authors using either manual or semi-automated processing procedures, and every processed record has passed a quality review by a trained analyst. Computed intensity measures include peak acceleration and velocity, pseudo-spectral acceleration response spectra, cumulative absolute velocity, Arias Intensity, and Fourier amplitude spectra. The processed time-series data, associated metadata, and ground motion intensity measures were organized into a web-served relational database consisting of 32 tables connected by primary/foreign key pairs. Ground …

Sustainable Management Of Erosive Shores: An Interdisciplinary Approach Integrating Engineering And Social Sciences At A Tide-Dominant Beach Area, Jun Ik Sohn, Hyun Dong Kim, Kiran Adhithya Ramakrishnan

Civil & Environmental Engineering Faculty Publications

This study investigates the causes and consequences of shoreline erosion at Kkotji Beach, a prominent tourist destination on the west coast of South Korea, where the degradation of the coastal environment has increasingly threatened the local tourism industry and economy, by employing a mixed-methods approach that combines field observations with MIKE 21 hydrodynamic simulations and by integrating perspectives from coastal engineering and the social sciences to develop practical, site-specific strategies for mitigating erosion, enhancing public awareness, and promoting sustainable coastal planning and development that support long-term environmental resilience and economic stability. The results show that dominant ebb currents drive southward …

Caveolin-Mediated Endocytosis: Bacterial Pathogen Exploitation And Host-Pathogen Interaction, Dibyasri Barman, Rishi Drolia

Biological Sciences Faculty Publications

Within mammalian cells, diverse endocytic mechanisms, including phagocytosis, pinocytosis, and receptor-mediated endocytosis, serve as gateways exploited by many bacterial pathogens and toxins. Among these, caveolae-mediated endocytosis is characterized by lipid-rich caveolae and dimeric caveolin proteins. Caveolae are specialized microdomains on cell surfaces that impact cell signaling. Caveolin proteins facilitate the creation of caveolae and have three members in vertebrates: caveolin-1, caveolin-2, and caveolin-3. Many bacterial pathogens hijack caveolin machinery to invade host cells. For example, the Gram-positive facultative model intracellular bacterial pathogen Listeria monocytogenes exploits caveolin-mediated endocytosis for efficient cellular entry, translocation across the intestinal barrier, and cell–cell spread. Caveolin …

Lap-Hsp60 Complex Modulates Epithelial Tight Junction Barrier, Arun Bhunia, Manalee Manalee Samaddar, Chen Sun, Nicholas Gallina, Nicole Irizarry-Tardi, Donqi Liu, Shivendra Tenguria, Rishi Drolia, Anika Jain, Daisuke Kihara, Wen Jiang, Kee-Hong Kim, Abigail Cox, Kurt Ristroph, Gregory Knipp, Nicholas Noinaj

Biological Sciences Faculty Publications

During infection, Listeria adhesion protein (LAP), a housekeeping enzyme, acts as a tight junction modulator (TJM) through interaction with Hsp60 to facilitate Listeria monocytogenes translocation across the intestinal epithelial barrier. Here, we used purified LAP as a potential TJM to overcome the limiting and variable effects observed by other agents in the class. We structurally determined the LAP interaction alone and in complex with Hsp60 utilizing cryo-EM and computational analysis. LAP structure resolved at 2.83 Å, forms multimeric interlocking dimers and tetramers, and the N-domain interacts with Hsp60, while the C-domain bridges the bacterial surface receptor InlB. The structural studies …

Low Salinities Impair Heat Tolerance And Alter Reproductive Traits With No Implications For Survival In A Marine Annelid From Coastal Habitats, Gabrielle Newton, Gloria Massamba-N'Siala

Biological Sciences Faculty Publications

Climate change is expected to intensify salinity and temperature fluctuations in estuarine and coastal habitats. However, the impact of salinity variation on ectotherms’ thermal tolerance remains underexplored, limiting our understanding of the interplay between these stressors. This study investigated salinity-dependent changes in life history traits (survival, growth rate, fecundity, and egg size) over a 33-day exposure to a salinity gradient (12, 17, 22, 27, 32, 35, 40, 45, 50) in the marine annelid Ophryotrocha labronica. Individuals that survived the salinity treatments were assessed for their upper and lower thermal tolerance limits. Female individuals, the target of the study, exhibited …

Cellular Responses Of Astrangia Poculata (Ellis And Solander, 1786) And Its Symbiont To Experimental Heat Stress, Tyler E. Harman, Daniel Barshis, Briana Hauff Salas, Kevin B. Strychar

Biological Sciences Faculty Publications

Climate change has had devastating effects on tropical coral reefs; however, much less is known regarding how heat stress affects temperate coral. This research focuses on Astrangia poculata (Ellis and Solander, 1786) collected from Narragansett Bay, RI, during the summer and winter seasons and understanding the effect of experimental thermal extremes (i.e., 26 °C) on seasonally different populations. Photosynthetic efficiency (Fv/Fm), symbiont density (via an inverse relationship with pixel intensity), and oxidative stress via reactive oxygen species (ROS) concentrations were measured on symbiotic and aposymbiotic A. poculata. Higher Fv/Fm rates were observed in summer- vs. winter-collected corals (p ≤ …