Digital Commons Network^™

Strategies To Improve Car-T Efficacy: Preconditioning, Gene Knockouts, And Enhancing Infiltration, Safa A. Khan, Salvina Alexander, Duy Anh Khuc, Tumaris Jarkinova, Fatema-Zahra Vhora, Neha Kunka, Rebecca Detweiler

OneUSF Undergraduate Research Conference

Chimeric Antigen Receptor T-cell (CAR-T) therapy is a powerful form of immunotherapy that has shown success in treating blood cancers. CAR-T cells often face challenges that limit their overall effectiveness; hence, researchers are focusing on strategies that improve how well CAR-T cells survive, grow, and target cancer cells. This review was conducted to analyze how CAR-T cells are constructed, highlight the major barriers that limit CAR-T’s efficacy, and determine how to improve it through preconditioning, gene knockouts, and enhanced infiltration. Literature was found through the PubMed, Scopus, and Web of Science databases. The following key search terms and synonyms were …

The Impact Of Ramadan Fasting On Chronic Disease Management, Abdulwahab Eddib, Aakez Adrak Marjan, Faris Jabbar, Nuzat Indawala

OneUSF Undergraduate Research Conference

Every year, 2.06 billion Muslims perform Ramadan fasting, a religious practice involving abstinence from food and drink from dawn to sunset for 30 days. This religious practice changes diet, hydration, sleep, and medication timing, raising clinical concerns for the safety of individuals with chronic diseases who choose to fast. Research reports mixed effects on disease control and treatment safety, emphasizing the need for an overarching analysis of its impact on chronic disease management. This literature review is aimed to examine research on Ramadan and intermittent fasting on chronic disease management and to analyze its effects on disease control, clinical outcomes, …

Comparative Analysis Of Car-T Cell Therapy Across Cancer Subtypes: Influence Of Antigen Binding Properties On Efficacy And Safety, Isabella Ware, Nadia Rohani, Kai Peñalosa, Samia Khan, Selena Berhie, Sreehitha Sajja

OneUSF Undergraduate Research Conference

CAR-T cell therapy is a personalized immunotherapy that genetically engineers a patient’s own immune T-cells in a lab to recognize and attack specific cancer cells. CAR-T cell therapy has shown remarkable success in hematologic malignancies, where antigen expression is highly uniform. The research question that was addressed is “To what extent does antigen heterogeneity and downregulation in solid tumors reduce CAR-T cell cytotoxicity compared with the more uniform antigen expression seen in hematologic malignancies?” CAR-T cell cytotoxicity is measurably reduced in solid tumors due to the tumor’s capacity to alter antigen expression and downregulate CAR proteins whereas hematologic malignancies exhibit …

Computational Analysis Of Gene Expression Profiles To Identify Novel Biomarkers For Triple-Negative Breast Cancer, Autumn Brooks, Saranya Sriramula, Sophia Chadwick, Julia Veronez Da Silva, Stecile Jean Mary, Sreehitha Sajja

OneUSF Undergraduate Research Conference

Our research focused on the effectiveness of using computational methods such as sequencing models to find biomarkers that confirm the existence of Triple-Negative Breast Cancer (TNBC) in patients. The research focused on analyzing the importance of being able to detect patterns in genomic sequences that were consistently seen in patients who were TNBC positive. Additionally, our aim was to understand resistance mechanisms that make TNBC harder to treat through sequencing analytics. Understanding these patterns is especially important for early intervention so patients who have TNBC can get started on treatment before the disease progresses. Triple-negative breast cancer lacks hormone receptors …

Reproductive Equity: A Comparative Chemical Analysis Of Emerging Male Contraception Technology, Grace Kim, Esmaralda Pilar-Lopez

OneUSF Undergraduate Research Conference

Female oral contraceptives, although abundant in access and successful as contraceptives, pose significant risks to women’s health. Biosocial factors that discourage male contraceptive drugs include harmful hormonal changes and inconsistent efficacy. This research employs comparative analysis of three current methods on potential male contraceptive options, focusing on steroid chemistry, functional group modification, and bioavailability. Emerging male contraceptive drugs of interest include the non-hormonal retinoic acid (vitamin A) receptor to antagonize sperm production, blocking the sperm-specific 〖Ca〗^(+2)  ion channel, and a reversible “Chemical Plug” that limits sperm movement at the vas deferens. Through comparison, this research will use six references to …

Islamic Prayer (Salah) And Stress Regulation: A Structured Review Of Biomarkers And Psychological Outcomes, Qiraat Shaikh, Nadean Shehadeh, Tulip Ghazi, Zaynaa Syed

OneUSF Undergraduate Research Conference

The relationship between Islamic prayer (Salah) and stress regulation has been extensively examined in Muslim populations because of its relevance to mental health. However, findings are dispersed across studies using varied methodologies. This structured literature review synthesizes existing research on Salah and stress reduction, focusing on outcomes such as changes in stress related biomarkers and self reported well being among Muslim populations. A structured literature search was conducted using PubMed, NIH, and Google Scholar. Studies published between 2015 and 2025 that focused on solely adult populations within Asia were selected. Studies that examine populations in both a clinical and non …

How Has Western Biomedical Framing Of Type 2 Diabetes Shaped Prevention And Treatment In South Asian Populations?, Amrita Nayak, Sreehitha Sajja

OneUSF Undergraduate Research Conference

This paper examines how Western biomedical framing of Type 2 diabetes (T2D) has shaped prevention and treatment strategies in South Asian populations, often with limited effectiveness. The dominant Western model emphasizes individual responsibility, standardized dietary guidelines, and generalized treatment protocols. While these approaches have proven useful in broader populations, they frequently fail to address the distinct biological, cultural, and structural realities that influence T2D risk among South Asians. A key limitation lies in the underrecognition of earlier metabolic risk. South Asian individuals tend to develop T2D at younger ages and lower body mass index (BMI) levels compared to European populations, …

Retrospective Analysis Of Fda Adverse Event Reporting System (Faers) For Herbal Supplements, Luke Junck, Jennifer Lin, Nikolas Reyes, Teba Albahely, Sreehitha Sajja

OneUSF Undergraduate Research Conference

FAERS is the national database maintained by the FDA in order to monitor the safety of drugs after they reach the market. FAERS supports post-marketing surveillance by helping regulators to detect emerging risks and guide decisions on product labeling changes or further investigations. A systematic review of 21 research articles was conducted, focusing on the population and reasonings behind adverse reports for herbal supplements. Articles were gathered from multiple databases such as Pubmed, ScienceDirect, SpringerNature, and government affiliated websites. This research explored the question, “How do adverse event reports for herbal supplements in FAERS differ across age, race, and geographic …

It's In Your Dna: A Comprehensive Overview Of Forensic Genetic Genealogy And The Future Of Profiling Methods And Legal Implications, Selah A. Thompson

OneUSF Undergraduate Research Conference

Forensic Genetic Genealogy (FGG) is a relatively modern and transformative technique within the field of forensics that allows for precise scientific identification of suspects or victims through DNA (Deoxyribonucleic Acid) analysis, primarily consisting of analyses of Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs) found within samples of DNA. While FGG is quite new within the field, the applications for accurate, scientific-based analysis of crime scenes can help make an unsolvable case something that can be resolved, including some of the most famous cold cases of the past century. Current FGG technology provides incredibly valuable investigative leads, but current …

Determination Of The Degree Of Channeling Present Between Separate Catalytic Domains In Rat Peptidyl-Glycine Alpha-Amidating Monooxygenase, Chloe E. Jones, Tyler Holets, David J. Merkler

OneUSF Undergraduate Research Conference

Rat Peptidyl-Gly Alpha-Amidating Monooxygenase, or PAM, is the ortholog of the enzyme in humans solely responsible for amidating bioactive peptides to enable full biological function. The enzyme is composed of two catalytic domains held together by a flexible linker. The first domain is the copper-containing peptidylglycine α-hydroxylating monooxygenase, or PHM, which α-hydroxylates the peptidyl-glycine substrates. The second domain is the zinc-containing peptidyl-α- hydroxyglycine α-amidating lyase, or PAL, which produces glyoxylate and the amidated peptide final product. In part due to the flexible linker between the domains, the mechanism of substrate transfer between PHM and PAL is poorly characterized. Channeling, or …

Telemedicine Vs. In-Person Oncology Care During Covid-19, Sarah C. Widlak, Genelle Leo, Moksh Patel, Hailey Menias, Ariadna Coa, Serena Bhaskar

OneUSF Undergraduate Research Conference

Amidst the COVID-19 pandemic, healthcare rapidly shifted towards telemedicine, raising questions about its efficacy. Although there is limited research, it is critical to understand how telemedicine affected oncology patients during the pandemic to improve patient care and experiences post-pandemic. This review addresses this gap by evaluating how telemedicine use among adult oncology patients during the pandemic compares with in-person visits regarding quality of care and patient satisfaction. A comprehensive search was performed across multiple databases, mainly PubMed, using keywords related to telemedicine, cancer, and COVID-19. The search yielded 106 unique articles, of which 41 met the inclusion criteria. Two rounds …

Exploring Stem Faculty Perceptions On The Barriers Of Community Engaged Learning, Chandni M. Rattansingh

OneUSF Undergraduate Research Conference

Calls to transform STEM education increasingly emphasize student-centered practices that move beyond passive lectures toward meaningful learning, preparing the future STEM workforce for complex, science-driven challenges. Community Engaged Learning (CEL) represents one such approach. While prior CEL research has largely focused on student outcomes, STEM faculty perceptions, especially regarding motivations, benefits, and barriers, remain understudied, despite faculty being central to CEL design and sustainability. This study investigates STEM faculty perceptions of the barriers to implementing CEL. We conducted 5 focus groups, each varying from 3-6 participants, intentionally separating adopters and non-adopters. Participants were STEM faculty from research-intensive universities holding the …

Cbt-Based Psychotherapy Apps Vs. No Intervention For Insomnia: A Systematic Review Of Symptom Differences In Dsm-5 Adults, Soham C. Dihenia, Mary Hanna, Melissa Fede, Aashna Patel, Pierre Mosad, Semené Clement, Amanda Janner

OneUSF Undergraduate Research Conference

Insomnia is a prevalent health issue impacting many adults alongside other mental disorders. One common treatment is cognitive behavioral therapy for insomnia (CBT-I) which is now increasingly delivered through smartphone apps. With an upward trend in adults, these applications offer a potential accessible and scalable solution. This study evaluated the efficacy of internationally available mental health apps utilizing CBT principles for adults diagnosed with insomnia under the DSM-5 which were compared to the control group with no psychotherapy app exposure.

A systematic review was conducted using PubMed, Google Scholar, and Scopus. Eligible studies were published within the last 5 years, …

The Effect Of Distraction On Emotion-Based Communication Behaviors, Twisha Nadella

OneUSF Undergraduate Research Conference

As technology becomes increasingly integrated in daily activities, there are growing concerns for the effects of multi-tasking within the workplace. It is important to consider how communication efficiency may be impacted by dual-task situations. Therefore, understanding the potential outcomes that distraction may have on language production is valuable in determining optimal working conditions for effective use of emotional communication. This dual-task study was designed to understand the effects of distraction on emotion-based communication behavior. A driving task was utilized as the means of distraction in this experiment, while the concurrent negotiation task was analyzed to understand how emotional language was …

To The Man I Once Was: Exploring Future Technology, Climate Change And Human Relationships In Our Future, Amanda Bitkauskaite

OneUSF Undergraduate Research Conference

From flying cars, evil corporations, killer robots and cybernetic enhancements, creatives have always liked to imagine what the future will be. This novella is no different, drawing from developing technology, climate change predictions and global political discourse to try and estimate what our future will look like.  Told as a simple ‘day in the life’ story, it follows a cyborg who saves a young human girl, taking her through what is an envisioned future of America; a country struggling with the effects of desertification, water crises, mass surveillance, wealth disparity and a growing divide between what it means to be …

Biased Agonism At Β2-Adrenergic Receptor For Therapeutic Precision, Miranda J. Miranda, Jinay Mehta, Zara Kazi, Pravarakhya Pottigari, Praveen Muthusamy, Ritesh Palla

OneUSF Undergraduate Research Conference

Immune and inflammatory disorders occur when the immune system becomes overactive, leading to ongoing inflammation and tissue damage. The β2-adrenergic receptor (β2-AR), found on many immune cells, helps regulate how immune cells move and respond. Traditional β2-AR drugs activate multiple signaling pathways at the same time, which can lead to inconsistent effects and off-target side effects such as increased heart rate, tremors, and reduced receptor sensitivity over time. This highlights the need for more targeted approaches. This systematic review examined whether biased agonism of β2-AR can selectively activate specific signaling pathways to better control immune responses and improve treatment outcomes. …

Optimization Of Antibiotic Selection Conditions In Human T-Cell Lines To Study Mechanisms Of Resistance To Bi-Specific Antibody Therapy In Multiple Myeloma, Raphael Gabra

OneUSF Undergraduate Research Conference

Multiple myeloma is a malignancy of plasma cells for which emerging therapies increasingly target B-cell maturation antigen (BCMA). Many experimental approaches used to investigate therapeutic resistance rely on genetically modified cell populations that must be maintained through antibiotic selection to ensure stable expression of introduced constructs. However, antibiotic sensitivity varies across cell lines and drug types, and improper dosing can compromise experimental reproducibility by allowing unmodified cells to persist or inducing excessive cytotoxic stress. This study aimed to define efficient and practical selection conditions for two commonly used antibiotics, G418 and puromycin, in Jurkat and TALL-104 human T-cell lines. Cells …

Maternal Feedback Over Time: Associations With Youth Perceptions Of Autonomy Support, Andrew Middleton, Genevieve Martin, Alyssa Lazanowski, Wendy M. Rote

OneUSF Undergraduate Research Conference

Parental feedback and direction are often interpreted as controlling; however, developmental theory suggests these behaviors may function as autonomy support when they are responsive to youths’ need. Drawing on self-determination theory, autonomy supportive parenting involves the promotion of volitional functioning through behaviors that align with youths’ perspectives and fosters independent problem-making and a sense of choice (McCurdy et al., 2020; Zhang & Whitebread, 2021). Similarly, scaffolding frameworks emphasize that provisions of parental support (e.g., feedback, direction, and emotional encouragement) contingent on the child’s task performance can promote higher levels of independent functioning (Mermelshtine, 2017; Salonen et al., 2007). The present …

A Momentary Examination Of Surface Acting And Its Impact On Subsequent Affective States Among College Students, Aditya D. Patel

OneUSF Undergraduate Research Conference

Though the psychological costs of surface acting, or the modification of outward emotional expression without concurrent alteration of genuine internal feelings, are well studied in service-oriented sectors, its impact on college students navigating social and academic demands is comparatively under-researched. This investigation seeks to examine the momentary and trait-level dynamics of surface acting and its impact on positive and negative affective states among collegiate populations. Observations were performed through a 5 day ecological momentary assessment protocol involving 72 undergraduate students, yielding over 1500 observations that were analyzed using multilevel vector autoregression to construct temporal, contemporaneous, and between-subjects networks. Temporal networks …

Localization And Function Of Tgsfa2 During Tachyzoite Division In Toxoplasma Gondii, Tom Goro

OneUSF Undergraduate Research Conference

Toxoplasma gondii is an obligate intracellular parasite infecting 30% of the global population. During host cell invasion, new parasites are formed through a budding process that partitions nuclei and organelles into daughters through a specialized form of division is known as endodyogeny. It involves closed mitosis, a single round of DNA replication, and coordinated organelle assembly within the mother cell. In cell division, centrosomes duplicate and serve as a central point for the reorganization of cytoskeletal structures known as microtubule organizing centers (MTOC). In typical eukaryotic cell division, centrosomes duplicate and migrate to opposite poles forming bipolar mitotic spindles required …

Gender Differences In The Diagnosis Of Histrionic Personality, Monica Hennawy, Heidi Lieu, Dayanna Gonzalez, Devin Dombroski, Kaylie Jusino Ortiz, Genelle Leo

OneUSF Undergraduate Research Conference

Histrionic Personality Disorder (HPD) is characterized by attention-seeking, emotionality, and dramatic or seductive behaviors. Individuals with HPD often feel discomfort when not the center of attention and may use exaggerated affect to gain approval. While gender differences in diagnosis and presentation have been reported, the influence of trauma and comorbid conditions - such as alcohol use disorder (AUD) and eating disorders (EDs) - remains underexplored. A literature review was conducted using PubMed with search terms including HPD, trauma, substance use, and eating disorders. A total of 157 articles were identified. Studies not addressing at least two relevant domains were excluded. …

The Overestimation Of Minority Group Size, Kennedy Tennant, Kiara Overstreet, Ty Curci

OneUSF Undergraduate Research Conference

Politicians and citizens often claim that a problem is "growing" or even "out of control" without providing metrics to support their claim. We wondered if people who fear immigration might be prone to overestimate the percentage of people who they perceive as "foreign" and further, whether that overestimation is moderated by the degree of fear or contempt held towards that group. In Study 1, participants viewed fictitious graduate school applications and then estimated the percentage of applicants that were from the Middle East. In study 2, participants viewed fictitious head shots and estimated the percentage of individuals who were wearing …

Leveraging Infection Control Practices To Maximize Quality Patient Outcomes, Sydney Busch, Lauren Rielly Mcconnell, Lauren Rae Selent

OneUSF Undergraduate Research Conference

Health care associated infections (HAI) remain a significant challenge for hospitals and may negatively affect patient outcomes if infection control practices are not consistently followed. A very important indicator of the safety and quality of health care is hand washing as it demonstrates the link between good hygiene practices and low health care related infection rates 1,2. This study examined how infection control practices differed across clinical units. A quantitative, causal- comparative design was used to identify differences across groups. The independent variable was the unit the patient was assigned to. Student researchers paired with faculty and infection control practitioners …

Cyclobenzaprine: Psychiatric Side Effects In Patients With Mood Disorders, Shelby R. Mcgrew, Michael Pestova-Habera

OneUSF Undergraduate Research Conference

Cyclobenzaprine, also known under the brand name Flexeril, is one of the most widely prescribed skeletal muscle relaxants globally. While treatment is typically limited to post-injury pain relief, long-term use has increased due to evidence of pain reduction in patients with chronic conditions such as fibromyalgia and temporomandibular joint disorder. Recently, cyclobenzaprine was found to be contraindicated for patients with bipolar disorder and other mood disorders. This is thought to result from its structural similarity to amitriptyline, a tricyclic antidepressant. As a result, some patients utilizing cyclobenzaprine for pain management may experience acute manic episodes following administration. This study reviews …

Park Architects: The Ocmulgee Mounds As A Unesco Sacred Natural Site, Ashley M. Swan, Layla Moss, Norah Mcelroy, Harley Jude Gilson, Lane Mellott

OneUSF Undergraduate Research Conference

The Ocmulgee Mounds in Macon, Georgia, were built around 900 BCE, representing indigenous land ties and cultural architecture in North America. Turning the Ocmulgee Mound site into a UNESCO Sacred Natural Site will ensure the site's protection, its cultural significance, educational opportunities, historical importance, and environmental resources. This research project will examine the cultural, historical, and environmental importance of the Ocmulgee Mounds and evaluate its eligibility for becoming a UNESCO Sacred Natural Site. The research findings show that the Ocmulgee Mounds site meets the required expectations for becoming a UNESCO Sacred Natural Site. Ocmulgee Mounds is a site that must …

Leveraging Cauti & Evidence-Based Best Practices, Jacob G. Slipak, Lauren Rae Selent, Lauren Reilly Mcconnell

OneUSF Undergraduate Research Conference

An estimated 44% of urinary tract infections are classified as catheter-associated urinary tract infections (CAUTIs). In 2020, stated United States CAUTI infection rates were estimated at 0.754 per 1000 catheter days1 (with higher rates in ICUs). CAUTI is associated with an adjusted incremental total hospital cost of $9,807 (p<0.0001) and increased length of stay of 3.01 days2 (p<0.0001). This study examines staff compliance with known CAUTI best practices. A quantitative, causal- comparative approach was utilized. All patients (n=119) had an indwelling foley catheter. The independent variables were the individual clinical units each patient was assigned to. The dependent variable was adherence to the CAUTI Prevention Bundle best practices. Best practices included proper removal protocol and catheter maintenance (ensuring an unobstructed flow). Initially, a MANOVA was used to compare differences across groups. For a statistically significant criteria one way ANOVA contrasts were conducted. There were statistically significant findings as evidenced by Wilk’s Λ (36, 472) = 0.53, p<0.0005. One-way ANOVA contrasts identified two statistically significant differences: 1) Less than half of employees (48%) were adhering to best practice guidelines for catheter removal protocol (p< 0.02); and 2) Employees (71%) were compliant with best practices for catheter care (p< 0.005). It was chilling to note that less than half of employees (48%) complied with the catheter removal process. This information is the first of its kind in CAUTI and may assist policymakers and other key stakeholders in Florida—and nationally—in identifying, designing and implementing strategies to promote best practices.

Teens And Technology Leverage Health Information Technology, Joshua Gerges, Emily Gerges, Lauren Rielly Mcconnell, Lauren Rae Selent

OneUSF Undergraduate Research Conference

Adolescents seeking health information have multiple sources including physicians, families, schools, organized activities outside of school, “the street” and the Internet. Teens struggle to decide which sources provide them with information that is understandable, particularly related to potentially risky health behaviors. The purpose of this study was to better understand how high-school students use electronic tools. A quantitative, causal comparative approach as used to identify differences across groups. The independent variables were the student grade level. The dependent variables were the students’ response to the following questions, Students who utilized: 1) a computer at home 2) the Internet at home; …

Crossing The Quality Communication Chasm Among Inpatients, Julie Gaurang Patel, Jacob Aiden Boyle, Lauren Reilly Mcconnell, Lauren Rae Selent, Joshua Refaat Gerges

OneUSF Undergraduate Research Conference

Identifying the most effective approach to enhancing service quality and patient satisfaction may be challenging for providers. Efficient communication between healthcare providers and patients is frequently mentioned as a fundamental element of favorable patient experiences. The purpose of this study was to examine patient satisfaction regarding ‘interpersonal communication’ within a multi-hospital system.  A quantitative, causal-comparative design was used to identify differences across groups. The independent variable was the floor the patient was admitted to. The dependent variables were the patients' responses to satisfaction survey questions including: 1) Friendliness of hospital staff; 2) Explanations provided in a way patients could easily …

Students And Obesity Awareness Shape Health Behaviors, Joshua Gerges, Emily Gerges, Lauren Rielly Mcconnell, Lauren Rae Selent

OneUSF Undergraduate Research Conference

Per the World Health Organization, obesity refers to an excessive fat accumulation posing a risk to one’s health. A body mass index (BMI) greater than 25 identifies individuals as overweight. BMI’s greater than 30 denotes obesity and associated with heightened mortality risk.1,2 A causal comparative design was used to identify difference across groups. The independent variable was the students grade level. The dependent variables were the students’ response to the following questions. Students who: 1) consider obesity a national health problem; 2) have been on a weight loss diet in the last year; 3) exercise 3X a week; 4) consume …

Anesthesiologists Shape Perioperative Clinical Decision-Making With Point Of Care Ultrasound (Pocus), Joshua Gerges, Emily Gerges, Lauren Reilly Mcconnell, Lauren Rae Selent

OneUSF Undergraduate Research Conference

POCUS is increasingly used by anesthesiologists during perioperative care to support clinical decision-making by providing rapid bedside imaging, POCUS can assist clinicians in evaluating patient status, identifying potential risks, and determining appropriate interventions. Understanding how anesthesiologists incorporate POCUS findings into their clinical decision-making processes is important in order to optimize perioperative care and guide future training and implementation of this technology. A Multivariate Analysis of Variance (MANOVA) was conducted to identify differences across groups. The independent variable was the type of POCUS examination. The dependent variables included:1) the patient was pre-op, 2) history was available, 3) overall risk potential, 4) …