Digital Commons Network^™

Humanized Saccharomyces Cerevisiae Provides A Facile And Effective Tool To Identify Damaging Human Variants That Cause Exosomopathies, Khondakar Sayef Ahammed, Milo B Fasken, Anita H Corbett, Ambro Van Hoof

Faculty, Staff and Student Publications

The RNA exosome is an evolutionarily conserved, multiprotein complex that is the major RNase in 3' processing and degradation of a wide range of RNAs in eukaryotes. Single amino acid changes in RNA exosome subunits cause rare genetic diseases collectively called exosomopathies. However, distinguishing disease-causing variants from nonpathogenic ones remains challenging, and the mechanism by which these variants cause disease is largely unknown. Previous studies have employed a budding yeast model of RNA exosome-linked diseases that relies on mutating the orthologous yeast genes. Here, we develop a humanized yeast model of exosomopathies that allows us to unambiguously assess damaging effects …

Faith-Based Charter Schools And The Establishment Clause: Like The Pig With Wings, They Look Good On Paper, But Can Or Should They Get Off Of The Ground?, Charles J. Russo

Louisiana Law Review

No abstract provided.

Age-Related Meningeal Extracellular Matrix Remodeling Compromises Cns Lymphatic Function, Kate Hitpass Romero, Taylor J Stevenson, Leon C D Smyth, Ben Watkin, Samuel J C Mccullough, Luca Vinnell, Amy M Smith, Patrick Schweder, Jason A Correia, Jonathan Kipnis, Mike Dragunow, Justin Rustenhoven

2020-Current year OA Pubs

Efficient clearance of central nervous system (CNS) waste proteins and appropriate immune surveillance is essential for brain health. These processes are facilitated by lymphatic networks present in the meninges that drain cerebrospinal fluid (CSF). Age-related impairments to meningeal lymphatic drainage contribute to CNS waste accumulation and immune dysfunction, yet the underlying mechanisms remain poorly understood. Here, we identify extracellular matrix (ECM) remodeling in the aged dura as a key driver of CSF clearance deficits, demonstrating that peri-lymphatic collagen accumulation disrupts lymphatic function. Exploring immune-derived factors contributing to this ECM remodeling, we identify transforming growth factor beta 1 (TGFβ1) as a …

Durability And Steel Corrosion Resistance Of Fly Ash Concrete Exposed To Groundwater, Mohamed Ahmed Bayomy, Khaled M. Osman, Hala Emad Elden Fouad

Mansoura Engineering Journal

This article proposes replacing Ordinary Portland Cement (OPC) with a fly ash (FA)-based geopolymer (GP) material, activated by a chemical activator, as the binding material. A 90-day study was conducted to evaluate the performance of fly ash geopolymer concrete (GPC) in a corrosive environment (groundwater). Scanning electron microscopy (SEM), compression testing, and energy-dispersive X-ray spectroscopy (EDX) were used to assess the toughness of the samples. Additionally, the linear polarization resistance (LPR) method was employed to measure the corrosion of steel embedded in the concrete structures. The comparison shows that increasing the fly ash (FA) content in geopolymer concrete (GPC) enhances …

A Nuclear Rna Degradation Code Is Recognized By Paxt For Eukaryotic Transcriptome Surveillance, Yoseop Yoon, Shuangyu Li, Lusong Tian, Marielle Valdez, Angela M Yu, Hong Yin, Wei Li, Fangyuan Ding, Georg Seelig, Lei Li, Yongsheng Shi

Faculty, Staff and Students Publications

The RNA exosome plays critical roles in eukaryotic RNA degradation, but how it specifically recognizes its targets remains unclear. The poly(A) tail exosome targeting (PAXT) connection is a nuclear adaptor that recruits the exosome to polyadenylated RNAs, especially transcripts polyadenylated at intronic poly(A) sites. Here, we show that PAXT-mediated RNA degradation is induced by the combination of a 5' splice site (ss) and a poly(A) junction (PAJ) but not by either sequence alone. These sequences are bound by U1 small nuclear ribonucleoprotein particle (snRNP) and cleavage/polyadenylation factors, which, in turn, cooperatively recruit PAXT. As the 5' ss-PAJ combination is typically …

A Deep Learning Strategy To Identify Cell Types Across Species From High-Density Extracellular Recordings, Marie E Hemelt, Federico D'Agostino, Marlies Oostland, Alvaro Sánchez-López, Young Yoon Chung, Michael Maibach, Stephen Kyranakis, Hannah N Stabb, M Gabriela Martínez Lopera, Agoston Lajko, Marie Zedler, Shogo Ohmae, Nathan J Hall, Beverley A Clark, Dana Cohen, Stephen G Lisberger, Dimitar Kostadinov, Court Hull, Michael Häusser, Javier F Medina

Faculty, Staff and Students Publications

High-density probes allow electrophysiological recordings from many neurons simultaneously across entire brain circuits but fail to reveal cell type. Here, we develop a strategy to identify cell types from extracellular recordings in awake animals and reveal the computational roles of neurons with distinct functional, molecular, and anatomical properties. We combine optogenetics and pharmacology using the cerebellum as a testbed to generate a curated ground-truth library of electrophysiological properties for Purkinje cells, molecular layer interneurons, Golgi cells, and mossy fibers. We train a semi-supervised deep learning classifier that predicts cell types with greater than 95% accuracy based on the waveform, discharge …

Intravascular Imaging-Guided Versus Angiography-Guided Percutaneous Coronary Intervention In Patients With Non-St-Segment Elevation Myocardial Infarction In The United States: Results From Big Data Analysis, Zhen Wang, Mahboob Alam, Hani Jneid, Samin Sharma

Faculty, Staff and Students Publications

Non-ST-segment elevation myocardial infarction (NSTEMI) can be managed by ischemia guide strategies or early invasive strategies. Here, we present the findings of an updated contemporary analysis regarding the use of intracoronary imaging (ICI)-guided PCI versus angiography-guided PCI and in-hospital mortality in patients with NSTEMI in the United States using the NIS database from 2016 to 2021. ICI use increased by nearly threefold between 2016 and 2021, without a significant difference in in-hospital mortality, though interestingly, mortality rates compared with angiography guidance were similar and relatively low. In this study, the use of ICI was associated with lower adjusted odds of …

Same Difference: A Qualitative Exploration Of Stressors Experienced By Dental Team Members Across The Uk, Uk Dental Team Mental Health Research And Implementation Group, Robert Witton, Jennifer Knights, Fiona Ellwood, Ian Mills, Lois Minnis

Peninsula Dental School

Introduction Poor mental health and wellbeing in dental team members is increasingly recognised. The practise of dentistry is linked to high levels of self-reported stress, burnout and psychological distress. Different dental systems operate across the four United Kingdom (UK) countries and there is currently a lack of exploration of the impacts these different systems have on the stressors and challenges dental teams experience.Aim The aim of this paper is to explore and provide a UK country comparison of dental team members' experiences and stressors reported through the MINDSET U.K. survey 2023.Method Qualitative data were collected in an online questionnaire which …

Genomic Insights And Phenotypic Characterisation Of Three Multidrug Resistant Cupriavidus Strains From The Cystic Fibrosis Lung, Sean D Kelly, James Butler, Heather Green, Andrew M Jones, Dervla T D Kenna, Sumita Pai, Katie J Muddiman, Trudie A Mccomb, Briana M Barrand, Vicky Bennett, Gyorgy Fejer, Mathew Upton

School of Biomedical Sciences

AimsWe aimed to investigate phenotypic and genomic traits of three Cupriavidus spp. isolates recovered from people with cystic fibrosis (PWCF). These bacteria are recognised as emerging pathogens in PWCF.Methods and ResultsUsing short and long sequencing reads, we assembled three hybrid complete genomes for the genus Cupriavidus, adding to the 45 published currently, describing multipartite genomes and plasmids. The isolates likely represent three different species, and they carry a cumulative total of 30 ARGs with high homology to well-characterised resistance determinants from other bacteria. Multidrug resistance to antibiotics used in CF management was observed in all three isolates. However, two treatments …

Asynchronous Development Of The Mouse Auditory Cortex Is Driven By Hemispheric Identity And Sex, Ashlan P Reid, Demetrios Neophytou, Robert Levy, Cody Pham, Hysell V Oviedo

2020-Current year OA Pubs

Lateralized auditory processing is essential for specialized functions such as speech processing, typically dominated by the Left Auditory Cortex (ACx) in humans. Hemispheric specializations also occur in the adult mouse ACx, but their developmental origins are unclear. Our study finds that the Left and Right ACx in mice reach developmental milestones at different ages. Thalamocortical responses and maturation of synaptic dynamics develop earlier in the Right ACx than the Left. We show that this timing offset predicts hemisphere-dependent differences in sensory-driven plasticity. Juvenile tone exposure at specific times results in imbalanced adult tone frequency representations in the Right and Left …

The Essential Calcium Channel Of Sperm Catsper Is Temperature-Gated, Dilip K Swain, Citlalli Vergara, Júlia Castro-Arnau, Polina V Lishko

2020-Current year OA Pubs

The flagellar calcium channel CatSper is essential for male fertility, as it regulates calcium influx to trigger the hyperactive motility required for sperm to fertilize the egg. Precise activation of CatSper is critical, as premature activation can impair sperm function. While optimal temperature is known to influence fertilization, its effect on CatSper remains unknown. By directly recording from mouse spermatozoa, we reveal that CatSper functions as a temperature-gated ion channel, with a thermal threshold of 33.5 °C and a temperature coefficient Q

The Geographic Identification Of Elevated Suicide Risk Model: Evaluating A Method For Examining Suicide-Related Behaviors At The Neighborhood Level In Harris County, Texas, Tyler Hendley, Jackson Bolner, Angela Cummings, Angie Hayes, Robert J Cramer

Faculty, Staff and Students Publications

Background: The 2024 National Strategy for Suicide Prevention has called for the development of community-based suicide prevention resources, and improved existing prevention efforts. In line with such efforts, Hill and colleagues developed the Geospatial Identification of Elevated Suicide Risk model that estimates the relative prevalence of adolescent suicide risk within specific geographical areas. The current study seeks to further evaluate and refine the model for use as a tool to evaluate risk and protective factors at the neighborhood level.

Method: Drawing from multiple sources, data was collected detailing adolescent suicidal ideation, suicide attempts, suicide fatalities, and census tract characteristics. Utilizing …

Dietary Patterns And Their Effects On C-Reactive Protein Regarding Female Reproductive Disorders, Mckenzie Moe

Theses and Dissertations

Objectives: Chronic inflammation, as indicated by elevated C-reactive protein (CRP), plays a critical role in women’s reproductive health disorders. CRP is one of the most common biomarkers of inflammation. Dietary components, lifestyle factors, and women’s reproductive health disorders are major components that influence the levels of CRP within the body. Other influential factors include injury, infection, and illness. The purpose of this review is to explore the findings of literature available on nutrition interventions for CRP levels in consideration of women's reproductive disorders.

Methods: This narrative review examines the works of 15 articles, including 10 cross-sectional and five longitudinal designs. …

The Role Of Almonds In Exercise Recovery And Athletic Performance, Kathryn M. Trochman

Theses and Dissertations

Objectives: During exercise, many trained athletes and physically active adults experience symptoms of exercise-induced muscle damage (EIMD). The effects of EIMD can cause limitations in athletic performance and increased duration of exercise recovery. Nutritional interventions have shown results in improving the duration of recovery. However, there are few studies completed on common food items. This review aims to examine the role of almonds in exercise recovery and athletic performance. Methods: A literature search was conducted utilizing Google Scholar, PubMed, CINAHL Ultimate, and reference hand searching. The search parameters included the dietary intervention of almonds for trained athletes and physically active …

The Virtual Content Viewing Device That Enables The User To Experience A Wider Virtual Content Viewing Area And At The Same Time That Enables The User To Experience The Perception Of A Wider Actual View Of The Real World., Punarjeewa Abeysekera

Defensive Publications Series

This paper describes a virtual content viewing device that enables the user to experience a wider virtual content viewing area and at the same time that enables the user to experience the perception of a wider actual view of the real world. The entire virtual content viewing device will consists of a composite projection configuration allocated for the left eye of the user and a composite projection configuration allocated for the right eye of the user. The composite projection configuration allocated for the left eye of the user, will contain a centrally located, from the top direction projecting, quadrilateral shaped …

A Historic Review And Empirical Revitalization Of The Stages Of Concern Questionnaire, Kent Anderson Seidel

School Leadership Review

This paper examines one of three central diagnostic tools of the Concerns Based Adoption Model, the Stages of Concern Questionnaire (SoCQ). The SoCQ was developed with a focus on K12 education. It has been used widely since developed in 1973, in early childhood, higher education, medical, business, community, and military settings. The SoCQ captures data about beliefs and intentions of individuals regarding their engagement with a new program or practice.

In our search of publications utilizing the SoCQ, we have found studies conducted across the world, with approximately 400 based in the United States 100 conducted outside the US. In …

Dismantling Inequities In The Faculty Evaluation System, Mary Petron, William Blackwell, Abbie Strunc

School Leadership Review

Qualifying for merit is a competitive and contentious process in higher education. Many merit structures are based upon traditional faculty evaluation systems which do not reflect the changing nature of higher education and thus, yield inequitable results. This article presents the case of a large department at a mid-size regional university that revised the system for merit and faculty evaluation to recognize undergraduate contributions and more equitably score the scholarship, service, and teaching of faculty members in the department. The case describes the relevant literature, the university and departmental context, the process for designing the new evaluation system, and lessons …

Can Kirana Stores Compete In India's E-Commerce Revolution? Rethinking Traditional Retail In The Digital Age, Anshi Jindal, Ashwarya Kapoor

Management Dynamics

E-commerce has reshaped the global retail landscape bringing both disruptions and opportunities specifically in emerging markets like India. This paper explores the evolution of e-commerce in India and its impact on traditional retail particularly unorganized Kirana stores. By analysing market trends, consumer behaviour and policy implications, the study examines how conventional retailers are adapting to digital transformation. Using Reliance Retail’s JioMart initiative as a case study, it provides insights into the integration of digital and physical retail ecosystems. While e-commerce challenges Kirana stores, it also fosters innovation, growth and the emergence of hybrid retail models.

A Parallel, Real-Time Fpga Implementation Of The Ccsds 123.0-B-2 Standard, Ruaa Ayman Saadi

Thesis/ Dissertation Defenses

Hyperspectral images are used in remote sensing scientific research and more, but they can reach hundreds of megabytes in size. This large number of datasets creates a problem with the limited storage capacity and bandwidth available on the satellite which in turn necessitates the use of hyperspectral image compression algorithms. The near lossless CCSDS123 hyperspectral algorithm provides a high compression capability, but it has some data dependencies in its predictor module that slows down the compression process. This Master of Science (MSc) thesis focuses on implementing a parallel, real-time FPGA based architecture of the near lossless CCSDS123 compression standard. The …

The Choice Of Law For Nazi-Looted Art Restitution: Cassirer V. Thyssen-Bornemisza Collection Foundation, California Assembly Bill 2867, And The Balance Between State, Federal, And Foreign Law, Alexander Stanfield

DePaul Journal of Art, Technology & Intellectual Property Law

No abstract provided.

A Deep Fake Dilemma: The Battle Over Keeping It Real, Tyler Gollin

DePaul Journal of Art, Technology & Intellectual Property Law

No abstract provided.

Machine Learned Misogyny: Gender Bias In Ai, Niria Rodriguez-Davila

DePaul Journal of Art, Technology & Intellectual Property Law

No abstract provided.

Training Your Large Language Model How To Speak "Human": A Transformative Public Necessity Or Copyright Infringement?, Isabelle Nasser

DePaul Journal of Art, Technology & Intellectual Property Law

No abstract provided.

Renaissance Of Inequitable Conduct: Luv N' Care, Ltd. V. Laurain And The Affirmative Egregious Misconduct Exception To But-For Materiality, Katherine Reich

DePaul Journal of Art, Technology & Intellectual Property Law

No abstract provided.

The Tax Adviser, Volume 17, Number 1, January 1986, American Institute Of Certified Public Accountants

Tax Adviser

No abstract provided.

Tax Trends, Nicholas J. Fiore

Tax Adviser

No abstract provided.

Thumbtax, Sandra K. Lewis

Tax Adviser

No abstract provided.

Tax Provisions Of Series E And Ee Government Bonds, Brenda Robert Hurst, Anthony P. Curatola

Tax Adviser

No abstract provided.

Tax Planning Using Electronic Spreadsheets On The Microcomputer, C. Douglass Izard

Tax Adviser

No abstract provided.

Tax Clinic, William T. Diss

Tax Adviser

No abstract provided.