Digital Commons Network^™

New Building Permits In The Mountain West, 2024, Taylor Volk, Mohit Pande, Kristian Thymianos, Caitlin J. Saladino, William E. Brown Jr.

Housing & Real Estate

This fact sheet examines data on new build permits from January 2023 to January 2024 in the five Mountain West States of Arizona, Colorado, Nevada, New Mexico, and Utah.“New Home Construction Statistics,” published by This Old House, reported percent changes on new builder permits and construction manufacturing costs and statistics across all 50 states.

Regulating Misdemeanors In China, Enshen Li

Brooklyn Journal of International Law

China has arguably entered an era of misdemeanors. Perceived as offenses punishable by a sentence of three years’ imprisonment or less, misdemeanors have reportedly taken up the majority of criminality in China over the past two decades. This shift in criminal dynamics has precipitated a pressing need for structural changes in the state’s criminal legal system, with calls for reform oriented toward decriminalization as a tailored dispositional channel for petty offenders showing a lower level of criminal culpability. This Article offers a critical scrutiny of China’s prevailing decriminalization initiatives for reducing the penalties and collateral consequences of misdemeanors. Situating these …

Questioning Us Immigration Law Compliance With Treaties For Trade And Investment, William Thomas Worster

Brooklyn Journal of International Law

This Article examines the extent to which US immigration law complies with the United States’ treaty obligations to admit qualifying foreign nationals as treaty traders (E-1) or treaty investors (E-2). These immigration categories are grounded in international agreements—specifically Friendship, Commerce and Navigation treaties (FCNs), Bilateral Investment Treaties (BITs), and Free Trade Agreements (FTAs)—that commit the United States to grant entry to certain foreign individuals and enterprises for the purposes of trade or investment. Although each treaty includes specifically tailored terms and conditions, US immigration regulations apply a single, harmonized set of regulations that often diverge from the treaty texts. This …

Sovereign Immunity And International Arbitration In China: One Step Forward, Two Steps Back, And Three Steps Forward Again, Ylli Dautaj

Brooklyn Journal of International Law

Investment treaty arbitration (ITA), like any other dispute resolution regime, must guarantee the quality if its means as well as offering effective sanctioning of its ends. Thus, any obstacle with respect to post-award proceedings should be dealt with seriously. Due to sovereign immunity, the enforcement against an award-debtor State is more difficult than against a private party. Courts in various jurisdictions have battled with sovereign immunity for many years now and many issues remain outstanding nevertheless. It is for these reasons that sovereign immunity from execution has been said to represent “the last fortress, the last bastion of State immunity.” …

Visa To Stay: Immigration Reform For International Students In The United States: From Contractual Limits To Affiliation-Based Opportunities, Jeanette Subkhanberdina

Brooklyn Journal of International Law

International student mobility is a vehicle of globalization in today’s world, with a significant rise in students pursuing higher education abroad over the past two decades, reaching approximately 6.9 million globally. Regardless of personal motivations, the decision to study abroad rests in a careful evaluation of whether long-term rewards outweigh the short-term sacrifices these students make. For students looking to build a professional foundation and immerse themselves in the culture of the country in which they study, few long-term rewards are more appealing than having their student visas serve as a pathway to permanent residency. Determining who may be granted …

Funding Repression: How The Eu Migration Agreements With Libya And Tunisia Circumvent Non-Refoulement And Enable Human Rights Violations, Emma Soltis

Brooklyn Journal of International Law

In 2023, the European Union and Tunisia entered into a Memorandum of Understanding, which outlined cooperation between the parties on several issues, including migration and mobility. Consistent with the European Union’s broader externalization agenda, the Memorandum of Understanding primarily allocates monetary and technical support to Tunisia and its authorities for migration management. In particular, Tunisian authorities are enabled to patrol the coast and intercept migrants and refugees attempting to irregularly migrate from North Africa to Europe across the Mediterranean Sea. The European Union sponsored a similar Memorandum of Understanding between Italy and Libya in 2017. Critics have widely denounced the …

The Starving Artist: What The Us Can Learn From Dutch Artist-Focused Legislation About Equitable Remuneration For Musical Creators, Natalia Perez-Flores

Brooklyn Journal of International Law

The United States (US) is the “leader in [the] creation, distribution, and worldwide licensing of recorded music.” Despite this, the US fails to afford moral rights protections to musical creators. Unlike economic rights, moral rights are not primarily concerned with the economic exploitation of art and instead protect the personal and reputational value of a work. Legislative justifications for copyright protection in the US include providing adequate rewards and appropriate incentives for artistic creation. To accomplish this, copyright law must protect authors’ personal expression, or moral rights, as much as it protects authors’ financial compensation, or economic rights. By focusing …

Beyond Statutory Loopholes, Qualified Immunity, And Internal Investigations: A Comparative Analysis Of Police Accountability In The United States And The United Kingdom, Kevyn Mcconlogue

Brooklyn Journal of International Law

While the landscape of policing in the United States and United Kingdom appear different today, their shared roots of enslavement and racism fostered a culture of impunity for police officers in both countries. With community skepticism and public discontent with law enforcement continuing to rise in the United States, the investigative and procedural roadblocks in the way of holding police officers accountable for misconduct must come to an end. In an effort to facilitate conversations about progressive police reform and eventual abolition, this Note compares the modern American and British police accountability systems and the avenues through which victims of …

The Role Of Environment In Conflict Discourse: Analyzing Nagorno-Karabakh, Mallory Klucken

University Honors Theses

This paper explores the role of ecological narratives in the long-standing conflict between Armenia and Azerbaijan over the Nagorno-Karabakh region in the South Caucasus. This paper argues that environmental narratives do not only reflect but also shape the conflict's trajectory--most notably in relation to Azerbaijan's 2023 territorial gains and their aftermath. Much of the existing scholarship focuses on the territorial, ethnic, and political dimensions of the conflict, however this study examines how environmental discourse has been employed by both sides to frame grievances and influence domestic and international opinion. By analyzing the discourse of ecocide and recent case studies, including …

Unsettling Home: Traces Of An Unrepaired Settler-Colonial Past In "Redding, California," 1846-1924, Celeste Hegy

University Honors Theses

This thesis critically reads primary documents from the settler-colonial past of "Redding, California," my hometown. In this paper, I sketch out a series of moments of historical trauma which continue to cry out for repair. Through a series of episodes from the mid-nineteenth through the early-twentieth centuries, I examine how original settler thirst for land wrought a contested hegemony in principally genocidal flows of power between the settler-state and its settler inhabitants and the pre-existing local Indigenous nations and peoples. In the first chapter, I argue for an understanding of the dispossession of the northern valley as genocidal. In the …

Incorporating Propensity Score Weighting And Nonresposne Adjustments Into Complex Survey Data With Survival Outcomes, Xinrui Shi

Theses and Dissertations

Propensity score weighting (PSW) plays a key role in minimizing confounding in observational research, especially when estimating treatment effects for time-to-event outcomes. However, its integration into survey data with complex design – particularly data with multiple stage sampling and censoring – remains underexplored. One significant challenge in such settings is the presence of nonresponse, which can introduce additional bias and complicate the use of standard weight adjustments. Moreover, there has been limited study on how PS weights can be effectively combined with nonresponse weighting adjustments in complex survey data that include survival outcomes. This dissertation aims to extend current methodologies …

Current Rates And Trends Of Venous Thromboembolism After Total Hip And Knee Arthroplasty: An Updated Analysis Utilizing The Nsqip Database, Alexander Van Der Ven

All Publications

Background: Total hip arthroplasty (THA) and total knee arthroplasty (TKA) procedure volumes are increasing. Venous thromboembolism (VTE) remains a significant complication, with incidence rates between 0.45% and 5.30%. Enhanced rapid-recovery pathways and chemoprophylaxis evolution may correlate with decreased VTE events over time. This study analyzes recent trends of VTE after THA and TKA.

Methods: Adults undergoing THA or TKA between 2009 and 2022 were identified from the National Quality Surgical Improvement Program database using Current Procedural Terminology codes. VTE was defined as the occurrence of a deep vein thrombosis (DVT) and/or pulmonary embolism (PE) event in the same patient. The …

The 2025 Sc Teacher Working Conditions Survey, Ruiqin Gao, Angela Starrett, Brian Cartiff, Svetlana Dmitrieva

Reports

The SC Teacher Working Conditions Survey (SCTWCS) was developed to better understand teachers’ experiences in their schools and the factors that infl uence their workplace satisfaction and career decisions. SC TEACHER designed the SCTWCS to include 10 areas of working conditions—six resources and four demands—with job satisfaction and intent to stay in the profession as outcomes. For novice teachers, three areas of mentor support (mentor instructional support, mentor non-instructional support, and proximity) are also included.

In the 2025 administration, 54 of the state’s 72 traditional school districts (75%) participated, along with f i ve of the six specialty schools and …

Black Soldier Fly, Hermetia Illucens, Larvae Meal Improves Intestinal Health And Growth Performance Of Nile Tilapia, Oreochromis Niloticus, Juveniles, Sahya Maulu, Ben Eynon, Sherilyn Abarra, Mark Rawling, Daniel Merrifield

School of Biological and Marine Sciences

This study investigated the effects of dietary black soldier fly (BSF) larvae, Hermetia illucens, meal on growth performance, feed utilization, intestinal health, and immune response in Nile tilapia, Oreochromis niloticus, juveniles. The fish (initial weight: 7.28 ± 0.09 g) were reared in a recirculatory aquaculture system (RAS) and fed diets containing different levels of defatted BSF larvae meal: 0% (control diet), 20% (BSF20), and 40% (BSF40). At the end of the feeding trial, the final body weight, weight gain, specific growth rate (SGR), protein efficiency ratio (PER), and feed conversion ratio (FCR) were significantly (p < 0.05) improved with increasing BSF meal inclusion levels in the diet. Histological analysis of the intestine revealed that mucosal fold length (MFL), muscularis thickness (MT), and goblet cell density (GCD) were significantly elevated by the BSF meal compared with the control. In addition, intraepithelial lymphocytes (IELs) and the enterocyte microvilli length were significantly elevated in BSF40-fed fish compared with the control. Gene expression analysis showed that intestinal chitin digestion-related (endochitinase A) and amino acid transporter (Slc6a18) genes were not significantly affected by the BSF meal. However, the expression of peptide transporter Slc15a1a was upregulated in the fish fed BSF40 compared to the control. Moreover, posterior intestine and head kidney cytokine (IL-1β, TNF-α, and IL-10) gene expression levels were not significantly affected by the inclusion of the BSF meal. Therefore, these results suggest that the BSF meal up to a 40% inclusion level can be used as an alternative protein source in the diets of Nile tilapia juveniles to promote growth and intestinal health.

Draft Final 2025 Residential Metals Abatement Program (Rmap) Quality Assurance Project Plan (Qapp) Annual Update (Residential Parcels), Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Specialized Insectivores Drive Differences In Avian Community Composition Between Primary And Secondary Forest In Central Africa, Eleanor M. Barrie, Billi A. Krochuk, Crinan Jarrett, Diogo F. Ferreira, Patricia F. Rodrigues, Susana Lin Mufumu, Silvestre Esteban Malanza, Agustin Ebana Nsue Akele, Cayetano Ebana Ebana Alene, Kristin Brzeski, Jacob C. Cooper, Jared Wolfe, Luke L. Powell

Michigan Tech Publications

The human population of sub-Saharan Africa is projected to triple by 2100, drastically increasing anthropogenic pressure on biodiversity. When rainforest is disturbed by anthropogenic drivers, species respond heterogeneously; these patterns have rarely been quantified for Congo rainforest fauna. Our objective was to understand how community composition changed with human disturbance—with particular interest in the guilds and species that indicate primary rainforest. At a long-term bird banding site on mainland Equatorial Guinea, we captured over 3200 birds across 6 field seasons in selectively logged secondary forest and in largely undisturbed primary forest. Our multivariate ordination indicated a significant split between primary …

Spatial And Multiomics Analysis Of Human And Mouse Lung Adenocarcinoma Precursors Reveals Tim-3 As A Putative Target For Precancer Interception, Bo Zhu, Pingjun Chen, Muhammad Aminu, Jian-Rong Li, Junya Fujimoto, Yanhua Tian, Lingzhi Hong, Hong Chen, Xin Hu, Chenyang Li, Natalie Vokes, Andre L Moreira, Don L Gibbons, Luisa M Solis Soto, Edwin Roger Parra Cuentas, Ou Shi, Songhui Diao, Jie Ye, Frank R Rojas, Eduardo Vilar, Anirban Maitra, Ken Chen, Nicolas Navin, Monique Nilsson, Beibei Huang, Simon Heeke, Jianhua Zhang, Cara L Haymaker, Vamsidhar Velcheti, Daniel H Sterman, Veena Kochat, William I Padron, Ludmil B Alexandrov, Zhubo Wei, Xiuning Le, Linghua Wang, Junya Fukuoka, J Jack Lee, Ignacio I Wistuba, Harvey I Pass, Mark Davis, Samir Hanash, Chao Cheng, Steven Dubinett, Avrum Spira, Kunal Rai, Scott M Lippman, P Andrew Futreal, John V Heymach, Alexandre Reuben, Jia Wu, Jianjun Zhang

Faculty, Staff and Student Publications

How tumor microenvironment shapes lung adenocarcinoma (LUAD) precancer evolution remains poorly understood. Spatial immune profiling of 114 human LUAD and LUAD precursors reveals a progressive increase of adaptive response and a relative decrease of innate immune response as LUAD precursors progress. The immune evasion features align the immune response patterns at various stages. TIM-3-high features are enriched in LUAD precancers, which decrease in later stages. Furthermore, single-cell RNA sequencing (scRNA-seq) and spatial immune and transcriptomics profiling of LUAD and LUAD precursor specimens from 5 mouse models validate high TIM-3 features in LUAD precancers. In vivo TIM-3 blockade at precancer stage, …

Role Of Interventional Pulmonology In Intensive Care Units: A Scoping Review, Abdul Rahman R Halawa, Saad Farooq, Mohammad Asim Amjad, Pushan P Jani, Sujith V Cherian

Faculty, Staff and Student Publications

Interventional pulmonology (IP) represents a rapidly growing and developing subspecialty within pulmonary medicine. To the intensivist, given the elaborate undertakings with respect to airway, lung and pleural disease management-IP has shown an increasing presence and remain a major ally in the care of these patients. Thus, an understanding of the different roles that IP could offer to the intensivist is of prime importance in the multi-disciplinary care of the complex patients within the intensive care units, particularly in relation to lung, airway and pleural diseases. This review article will explore the different intersections of IP in critical care and discuss …

Radiotherapy Promotes Cuproptosis And Synergizes With Cuproptosis Inducers To Overcome Tumor Radioresistance, Guang Lei, Mingchuang Sun, Jun Cheng, Rui Ye, Zhengze Lu, Amber Horbath, David Huo, Shengrong Wu, Anagha Alapati, Sadhna Aggarwal, Zhihao Xu, Chao Mao, Yuelong Yan, Jun Yao, Qidong Li, Xiong Chen, Hyemin Lee, Li Zhuang, Dadi Jiang, Apar Pataer, Jack A Roth, Nicholas Navin, Albert C Koong, Mingjian James You, Steven H Lin, Boyi Gan

Faculty, Staff and Student Publications

Cuproptosis is a recently identified form of copper-dependent cell death. Here, we reveal that radiotherapy (RT) induces cuproptosis in cancer cells, independent of apoptosis and ferroptosis, and depletes lipoylated proteins and iron-sulfur (Fe-S) cluster proteins-both hallmarks of cuproptosis-in patient tumors. Mechanistically, RT elevates mitochondrial copper levels by upregulating copper transporter 1 (CTR1) and depleting mitochondrial glutathione, a copper chelator, thereby triggering cuproptosis. Integrated analyses of RNA sequencing (RNA-seq) from radioresistant esophageal cancer cells and single-cell RNA-seq from esophageal tumors of patients unresponsive to RT link radioresistance to the downregulation of BTB and CNC homology 1 (BACH1). This downregulation de-represses the …

Radiotherapy Promotes Cuproptosis And Synergizes With Cuproptosis Inducers To Overcome Tumor Radioresistance, Guang Lei, Mingchuang Sun, Jun Cheng, Rui Ye, Zhengze Lu, Amber Horbath, David Huo, Shengrong Wu, Anagha Alapati, Sadhna Aggarwal, Zhihao Xu, Chao Mao, Yuelong Yan, Jun Yao, Qidong Li, Xiong Chen, Hyemin Lee, Li Zhuang, Dadi Jiang, Apar Pataer, Jack A Roth, Nicholas Navin, Albert C Koong, Mingjian James You, Steven H Lin, Boyi Gan

Faculty, Staff and Student Publications

Cuproptosis is a recently identified form of copper-dependent cell death. Here, we reveal that radiotherapy (RT) induces cuproptosis in cancer cells, independent of apoptosis and ferroptosis, and depletes lipoylated proteins and iron-sulfur (Fe-S) cluster proteins-both hallmarks of cuproptosis-in patient tumors. Mechanistically, RT elevates mitochondrial copper levels by upregulating copper transporter 1 (CTR1) and depleting mitochondrial glutathione, a copper chelator, thereby triggering cuproptosis. Integrated analyses of RNA sequencing (RNA-seq) from radioresistant esophageal cancer cells and single-cell RNA-seq from esophageal tumors of patients unresponsive to RT link radioresistance to the downregulation of BTB and CNC homology 1 (BACH1). This downregulation de-represses the …

Determining Accurate Pore Structures Of Polypropylene Membrane For Ecmo Using Fe-Sem Under Optimized Conditions, Makoto Fukuda, Yoshiaki Nishite, Eri Murata, Koki Namekawa, Tomohiro Mori, Tsutomu Tanaka, Kiyotaka Sakai

Children’s Nutrition Research Center Staff Publications

Long-term ECMOs are expected to be put into practical use in order to prepare for the next emerging severe infectious diseases after the novel coronavirus pandemic in 2019-2023. While polypropylene (PP) and polymethylpentene (PMP) are currently the mainstream materials for the hollow fiber membranes of ECMO, the PP membrane coated with a silicone layer on the outer surface has also been commercialized. In this study, we sought a method to accurately observe the detailed pore morphologies of the PP membrane by suppressing irreversible changes in the morphology in SEM observation, which is a general-purpose observation with higher resolution. As a …

Angela Asante And Anita Asante As Administrators Of The Estate Of John Asante V. Westfield

All Decisions

In a holdover proceeding, the tenant moved to dismiss for lack of standing, asserting the landlord, as administrators, transferred property title to themselves individually prior to commencement. The court found that a proceeding initiated by an improper party is a nullity and cannot be cured by amendment or substitution, as standing must exist at the time of commencement. Since the estate did not own the premises, the proceeding was dismissed without prejudice, denying the landlord's motions to amend and substitute as moot.

Justo-Aponte V. Eshcol

All Decisions

Handwritten opinion. In an illegal lockout case, the court granted the tenant's motion for civil contempt against the landlord, who ignored a judgment of possession. The tenant was awarded $1,264.00 for eight nights of hotel costs at $158.00 per night, plus a $250.00 statutory fine and attorneys' fees. The landlord eventually complied with the court's order by installing a door and providing the tenant with a key after multiple court appearances.

Current Cutting-Edge Omics Techniques On Musculoskeletal Tissues And Diseases, Xiaofei Li, Liang Fang, Sade W Clayton, Dakota R Kamm, Cuicui Wang, Farshid Guilak, Simon Y Tang, Gretchen A Meyer, Jie Shen, Et Al.

2020-Current year OA Pubs

Musculoskeletal disorders, including osteoarthritis, rheumatoid arthritis, osteoporosis, bone fracture, intervertebral disc degeneration, tendinopathy, and myopathy, are prevalent conditions that profoundly impact quality of life and place substantial economic burdens on healthcare systems. Traditional bulk transcriptomics, genomics, proteomics, and metabolomics have played a pivotal role in uncovering disease-associated alterations at the population level. However, these approaches are inherently limited in their ability to resolve cellular heterogeneity or to capture the spatial organization of cells within tissues, thus hindering a comprehensive understanding of the complex cellular and molecular mechanisms underlying these diseases. To address these limitations, advanced single-cell and spatial omics techniques …

Case Report On Acute Hemorrhagic Acute Disseminated Encephalomyelitis (Adem) Following Parvovirus B19 Infection In An Immunocompetent Child, Akash Dnyandeo Sarkate, Sushma Save, Alpana Kondekar, Raja Rajan

Digital Journal of Clinical Medicine

Acute disseminated encephalomyelitis (ADEM) is a rare, immune-mediated demyelinating disorder of the central nervous system, often triggered by viral infections. Parvovirus B19 has been implicated in various haematological and neurological manifestations; however, its association with acute hemorrhagic ADEM remains rare. Here, we report a case of a 5-year-old male immunocompetent child who developed ADEM following parvovirus B19 infection, emphasising the rarity of such presentations and the importance of early recognition and management.

Targeting Detoxifying Enzymes In The Lymphatic Filariasis Vector: An In Silico Study On Curcumin, Camphor, And Menthol, Rizal Subahar, Rawina Winita, Surya Dwira, Gulshan Fahmi El Bayani

Indonesian Journal of Medical Chemistry and Bioinformatics

Vector control remains a critical component in the prevention of lymphatic filariasis, a disease transmitted by insect vectors. Natural compounds such as curcumin, camphor, and menthol are being explored for their bio-insecticidal properties due to their potential to inhibit key detoxification and neurological enzymes in insects, including acetylcholinesterase (AChE), glutathione S-transferase (GST), and cytochrome P450 oxidases (CYP450). A molecular docking study using SwissDock was conducted to evaluate the interaction of curcumin, camphor, and menthol with AChE, GST, and CYP450 enzymes. Binding affinity (ΔG), hydrogen bonding, and active site interactions were analyzed to assess the inhibitory potential of each compound. Curcumin …

Designing For Dissemination Through Community Advisory Board Engagement In An Implementation Mapping Process: A Case Study, Gabriella M Mcloughlin, Yerusalem Yohannes, Molly Kerstetter, Omar Martinez, Alex Dopp, Jennifer O Fisher, Maura Kepper, Rachel G Tabak, Ross C Brownson

2020-Current year OA Pubs

INTRODUCTION: The purpose of this study was to document the development of a Community Advisory Board (CAB) to enhance equitable dissemination of research findings within an implementation mapping study to enhance equitable impact of Universal School Meals (USM) through the Designing for Dissemination and Sustainability (D4DS) process.

METHODS: The D4DS process comprises 7 key elements to facilitate meaningful dissemination. To accomplish Step 1: Identify Partners, the research team conducted snowball recruitment methods within the local Philadelphia community and with existing connections. To Empathize and Outline the Problem (Step 2) and Understand the Context (Step 3), an interest meeting was held …

A Provably Secure Coercion-Resistant E-Voting Scheme With Confidentiality, Anonymity, Unforgeability, And Cai Verifiability, Yun Xing Kho, Swee Huay Heng, Syh Yuan Tan, Ji Jian Chin

School of Engineering, Computing and Mathematics

Ensuring both cast-as-intended (CAI) verifiability and coercion-resistance in e-voting remains a critical challenge. The e-voting scheme proposed by Finogina and Herranz in 2023 represents the first notable advancement in reconciling these conflicting requirements. CAI verifiability allows voters to confirm that their intended vote has been correctly recorded, even without a secure channel to the election committee, while coercion-resistance prevents external influence and vote-selling. However, essential security properties such as confidentiality, anonymity, unforgeability, and double-voting prevention fall outside the scope of Finogina and Herranz’s e-voting scheme, leaving significant gaps in its security guarantees. To address this limitation, we propose a novel …

Identifying The Drivers Behind The Decline Of Douglas-Fir And True Fir Species In Sw Oregon, Alexander Bryant Bentley

Dissertations and Theses

Increased levels of Pseudotsuga menziesii (DF) and Abies species (TF) decline throughout dry forests in the Klamath Mountains and Western Cascades ecoregions have been recorded in recent years, with preliminary evidence suggesting that factors influenced by changing climatic conditions and encroachment from fire suppression policies are primarily to blame. Yet, the physiological and environmental mechanisms of drought-driven tree decline are poorly understood across biophysical and spatial gradients. Given the complex relations that drive decline, determining the degree of influence of specific drivers is difficult, creating unique challenges for modern forest management. To elucidate the most important factors driving DF and …

Lrrc8 Channel Complexes Counterbalance Katp Channels To Mediate Swell-Secretion Coupling In Mouse Pancreatic Β Cells, Tarek Mohamed Abd El-Aziz, Chen Kang, Litao Xie, John D. Tranter, Sumit Patel, Rahul Chadda, Maria S. Remedi, Rajan Sah

2020-Current year OA Pubs

Insulin secretion from pancreatic β cells is initiated by membrane potential depolarization, followed by activation of voltage-gated Ca2+ channels to trigger Ca2+-mediated insulin vesicle fusion with the β cell plasma membrane. Here, we show that β cell swelling associated with glucose metabolism was sensed by LRRC8 channel complexes and contributed to insulin secretion. Hypertonic perfusate (360-380 mOsm) dose dependently impaired glucose-stimulated insulin secretion by counteracting β cell swelling. Hypotonic perfusate alone, independent of glucose stimulation or KATP channel closure, was sufficient to increase β cell intracellular Ca2+ and trigger insulin secretion. Inhibition of sodium-potassium-chloride cotransporter-1 with bumetanide, which diminished the …