Digital Commons Network^™

Green Synthesis, Characterization, Antimicrobial And Anticancer Studies Of Vanadium Oxide Nanoparticles Using Vitex Agnus Castus Extract, Saba H. Mahdi, Lekaa K. Abdul Karem

Baghdad Science Journal

This study, vanadium oxide nanoparticles were synthesized using the biosynthesis method. The resulting VO₂ NPs are characterized by their cost-effectiveness, ease of use, and safety. Vanadium Nano oxide was prepared from vanadium sulfate monohydrate with a plant extract of Vitex agnus castus at a sodium hydroxide concentration of 0.1 M. The reaction was carried out in an alkaline medium at pH range of 8–12, which were characterized by using many techniques such as; Fourier transform IR spectroscopy, ultraviolet-visible spectroscopy, x-ray diffraction XRD, transmission electron microscopy TEM, scanning electron microscopy SEM and EDX pattern. The crystal size of nanoparticles, determined …

Potential Epigenetic Impacts Of Phytochemicals On Ruminant Health And Production: Connecting Lines Of Evidence, Sebastian P. Schreiber, Juan Villalba, Mirella L. Meyer-Ficca

Wildland Resources Student Research

The once mysterious “dark matter of nutrition”, comprising countless plant derived secondary compounds, also known as phytochemicals, is now understood to have significant and wide-ranging effects on consumers, including myriad health benefits in humans and livestock. The selective consumption of phytochemically rich and diverse plants, in appropriate doses, by ruminants represents an adaptive means of therapeutic and prophylactic self-medication. Due to their chemical structure, phytochemicals have long been recognized as antioxidants. However, the mechanisms that underlie numerous additional phytochemical-based health benefits are generally less understood. These effects (i.e., anti-inflammatory, immunomodulatory, and anticarcinogenic effects) are likely related to epigenetic processes. Evidence …

Rapid Beamformer Weight Calibration Update Method For The Advanced L-Band Phased Array Camera For Astronomy (Alpaca), Kayla Lyman Ward

Theses and Dissertations

The Advanced L-Band Phased Array Camera for Astronomy (ALPACA) is a phased array feed instrument designed for the Green Bank Telescope. It is capable of producing 40 real-time beams on the sky, widening the telescope's field of view and significantly increasing survey speed. ALPACA employs an RF-over-fiber (RFoF) signal transport system from the phased array feed front end to the digital backend. This RFoF link is expected to introduce phase delays and changes in gain through out the course of an observation, invalidating the beamformer weights previously calculated. This work presents rapid beamformer weight calibration updates for ALPACA, a method …

Slow-Growing Lung Mass In A Patient With Multiple Endocrine Neoplasia Type I: Consider Carcinoid Tumor, Ramin Talebi, Charalambos C. Solomides, Nathaniel R. Evans Iii, Gregory C. Kane

Division of Pulmonary, Allergy, and Critical Care Medicine Faculty Papers

A 23-year-old man with multiple endocrine neoplasia type 1 (MEN 1) with pancreatic tail masses and Cushing syndrome (status after transsphenoidal adenomectomy) presented with a slow-growing, asymptomatic right lower lung lobe mass confirmed to be an atypical carcinoid tumor by pathologic staging following successful basilar segmentectomy with mediastinal lymphadenectomy. Pulmonary carcinoid tumor (PCT) is a rare neoplasm in patients with MEN 1, but there is limited data on trends in atypical versus typical carcinoid tumors in these patients. This case emphasizes the need to characterize these trends given the implications for metastatic potential and 5-year survival, which can help guide …

A Systematic Evaluation Of Threaded Internode Communication In Hpc, William Pepper Marts

Computer Science ETDs

High Performance Computing (HPC) applications increasingly rely on both process and thread-level parallelism to maximize performance across complex, multi-node systems. However, conventional bulk synchronous communication strategies often leave both compute and network resources underutilized due to synchronization delays. This dissertation systematically evaluates the potential of fine-grained, threaded inter-node communication as a strategy for reducing these inefficiencies. To this end, I design and develop two tools: the MiniMod modular application framework and the Configurable Messaging Benchmark (CMB), which together enable empirical, reproducible assessment of communication performance across varying application behaviors, threading models, and communication granularities. Through experiments across multiple systems and …

The Effect Of Dbd-Non Thermal Plasma On Oxidative Stress And Tac In Patients With Hcv Infection, Salima K. Ahmed, Salwa H. N. Al- Rubae’I, Hamid H. Murbat, Mazin R. Mohammed

Baghdad Science Journal

Non-thermal plasma (NTP) is a processing technique that is widely-spread in different biological and medical application which stimulates or inhibits cellular processes through releasing reactive oxygen and nitrogen species (ROS and RNS, respectively) in biological systems. In this study, it was investigated that the impact of NTP on oxidative stress and antioxidant capacity markers, as well as the effect of NTP on the copy number of Hepatitis C virus (HCV RNA). Dielectric barrier discharge (DBD) plasma was used to generate the NTP. The results showed that the exposure of HCV specimen to NTP causes oxidative stress, RNA damage, Protein denaturation, …

Identification Of Hypoxia-Inducible Factor Hif1a As A Therapeutic Target During Sars-Cov-2-Associated Lung Injury, Bentley Bobrow, Samuel D Luber, Paul Potnuru, Katherine Figarella, Jieun Kim, Yanyu Wang, In Hyuk Bang, David Robinson, Paulina B Sergot, Steven K Burke, Tingting Mills, Constanza De Dios, Robert Suchting, George W Williams, Adit A Ginde, Yafen Liang, Hongfang Liu, Charles Green, Marie-Francoise Doursout, Alparslan Turan, Daniel I Sessler, Xiaoyi Yuan, Holger K Eltzschig

Faculty, Staff and Student Publications

Hypoxia-inducible factors (HIFs) promote lung protection and pathogen eradication during acute lung injury. We therefore tested the theory that pharmacologic stabilization of HIFs dampens lung injury during SARS-CoV-2 pneumonia. Initial studies in murine SARS-CoV-2 models showed improved outcomes after treatment with the FDA-approved HIF-stabilizer vadadustat. Subsequent studies in genetic models implicated alveolar-expressed Hif1a in mediating lung protection. Therefore, we performed a randomized, double-blinded, multicenter phase 2 trial in patients admitted for SARS-CoV-2 infection and concomitant hypoxia (SpO2 ≤ 94%). Patients (n=448) were randomized to oral vadadustat (900 mg/day) or placebo for up to 14 days. Safety events were similar between …

Staff Education To Addressing Medication Nonadherence Amongst Patients With Mental Health Challenges, Euphemia Mah

Walden Dissertations and Doctoral Studies

Walden University College of Nursing This is to certify that the doctoral study by Euphemia Mah has been found to be complete and satisfactory in all respects, and that any and all revisions required by the review committee have been made. Review Committee Dr. Corinne Wheeler, Committee Chairperson, Nursing Faculty Dr. Robert McWhirt, Committee Member, Nursing Faculty Chief Academic Officer and Provost Sue Subocz, Ph.D. Walden University 2025 Summary Poor adherence to medication regimens among patients who have a mental illness leads to multiple adverse outcomes, which include symptom exacerbation, decreased quality of life, and repeated hospitalizations. To address this …

Self-Initiated Police Activity, Crime, And Complaints Against Police, Vincent Joseph Schaan

Walden Dissertations and Doctoral Studies

This quantitative study aimed to examine the statistical relationship between self-initiated police activity, citizen complaints against police officers, sustained findings of misconduct, and crime within three police agencies located in the southwest United States. Prevailing law enforcement perception fueled by protests and riots infers that reducing proactive policing will decrease complaints. Using chi-square and binary logistic regression, this study analyzed 53,585 police and public contacts to answer three core questions. The statistical tests found a statistically significant inverse relationship between self-initiated police activity and complaints against police, with an odds ratio of 2.386 (95% CI: 1.62,3.50; p<.001). The findings indicate that officers who engage in self-initiated police activity have a 35.95% lower statistical probability of facing a citizen complaint. This study also found no statistically significant relationship between actual police misconduct and self-initiated police activity. The findings advocate for evidence-based decision-making based on statistical data, which found a reduced probability of complaints when law enforcement officers initiate public and police interactions. These findings contribute to positive social change by providing critical knowledge and information, and creating the potential for law enforcement officers and agencies to improve their relationships with the public by reducing complaints. Increasing self-initiated police activity provides a gateway to a reduction in crime and fewer complaints against police; therefore, data and information may motivate agencies and officers to increase self-initiated police activity to help reduce complaints and reduce crime.

Supporting Achievement Motivation In Black And Latine Middle School Students: Development And Evaluation Of The Feasibility And Acceptability Of A Motivational Interviewing Intervention, Austin Roy Cole

USF Tampa Graduate Theses and Dissertations

Participation in accelerated high school curricula can provide substantive post-secondary benefits for students. However, participation continues to reflect disparate enrollment for Black and Latine students. Though organizations and legislatures have sought to improve Black and Latine student enrollment, the enrollment gap has not closed and there is little evidence of improving academic achievement for Black and Latine students beyond increased enrollment. Research shows that when students have their psychological needs of autonomy, competence, and relatedness met, as described by Self-Determination Theory, they demonstrate better academic outcomes and social-emotional development. Barriers reported by students of color reflect these psychological needs. Thus, …

A Community-Based Study To Investigate Persistent Ambient Odors In Childs Park, Fl, Gennaro Saliceto

USF Tampa Graduate Theses and Dissertations

For many years, residents in Childs Park, Saint Petersburg, Florida, have reported unpleasant odors. Although there have been prior efforts to address the issue, the odors continue to be a concern for the community, who worry about possible health implications. This research aimed to equip residents with information to address the issue by identifying the presence of airborne pollutants that generate odors, quantifying the concentrations of toxic organic pollutants, and exploring the sources and environmental conditions that contribute to odors and their perception.

To accomplish these aims, the study utilized different methods. First, two walking measurement campaigns were conducted to …

X-Linked Hypophosphatemia Management In Children: An International Working Group Clinical Practice Guideline., Dalal S. Ali, Thomas O. Carpenter, Erik A. Imel, Leanne M. Ward, Natasha M. Appelman-Dijkstra, Catherine Chaussain, Suzanne M. Jan De Beur, Pablo Florenzano, Hajar Abu Alrob, Rana Aldabagh, R Todd Alexander, Farah Alsarraf, Signe Sparre Beck-Nielsen, Martin Biosse-Duplan, Rachel K. Crowley, Karel Dandurand, Guido Filler, Lisa Friedlander, Seiji Fukumoto, Claudia Gagnon, Paul Goodyer, Corinna Grasemann, Chelsey Grimbly, Salma Hussein, Muhammad K. Javaid, Sarah Khan, Aneal Khan, Anna Lehman, Willem F. Lems, E Michael Lewiecki, Ciara Mcdonnell, Reza D. Mirza, Emmett Morgante, Archibald Morrison, Anthony A. Portale, Christina Rao, Yumie Rhee, Eric T. Rush, Heide Siggelkow, Sotirios Tetradis, Laura Tosi, Gordon Guyatt, Maria Luisa Brandi, Aliya A. Khan

Manuscripts, Articles, Book Chapters and Other Papers

CONTEXT: An International Working Group (IWG) developed new guidelines on the diagnosis, evaluation, management, and monitoring of X-linked hypophosphatemia (XLH) in children. Over the past 5 years, important advances have occurred in our understanding of the presentation, complications, and treatment of XLH.

METHODS: A group of 50 international experts in XLH from Canada, the United States, Europe, Asia, and South America, along with methodology experts and a patient partner, held 18 teleconference meetings in 2023-2024. These meetings addressed key issues regarding diagnosing, evaluating, managing, and monitoring XLH in children. Two systematic reviews were conducted to examine the impact of burosumab …

Filtration Challenges In Non-Alcoholic And Low-Alcohol Beer Production With A Focus On Different Yeast Strains, Christian Schubert, Joerg Maxminer, Matthew Aitkens, Andrew Maust, Bernardo Pontes Guimaraes, Rahul Sen, Scott Lafontaine

Food Science Faculty Publications and Presentations

This study examines the impact of yeast selection on fermentation performance, filtration efficiency, and the stability of low-alcohol and non-alcoholic beer (NAB). Three yeast strains (LA-01, LoNa, and W-34/70) were evaluated for their effects on key NAB parameters like ABV, bitterness, color, haze readings, and filtration-linked performance. Filtration trials revealed that highly flocculating W-34/70 resulted in lower post-filtration turbidity, whereas LA-01 and LoNa (which flocculated less) required more effective filtration techniques to achieve clarity. Among the tested filter sheets, AF 31, AF 71, and AF 101 successfully reduced turbidity below 2 EBC. However, all NABs exhibited haze formation during storage, …

The Maturity-Onset Diabetes Of The Young (Mody) Calculator Overestimates Mody Probability In Hispanic Youth, Guido Alarcon, Anh Nguyen, Angus Jones, Beverley Shields, Maria J Redondo, Mustafa Tosur

Children’s Nutrition Research Center Staff Publications

Context: The applicability of the maturity-onset diabetes of the young (MODY) risk calculator to non-White European populations remains unknown.

Objective: We aimed to test its real-world application in Hispanic youth.

Methods: We conducted a retrospective chart review of Hispanic youth (< 23 years) with diabetes (n = 2033) in a large pediatric tertiary care center in the United States. We calculated MODY probability for all subjects, splitting them into 2 cohorts based on the original model: individuals who were started on insulin within 6 months of diabetes diagnosis (Cohort 1) and those who were not (Cohort 2).

Results: Cohort 1 consisted of 1566 individuals (median age [25p, 75p]: 16 [13, 19] years, 49% female), while Cohort 2 comprised 467 youth (median age [25p, 75p]: 17 [15, 20] years, 62% female). The mean MODY probability was 5.9% and 61.9% in Cohort 1 and Cohort 2, respectively. The mean probability for both cohorts combined was 18.8%, suggesting an …

Changes In Aggressive Behaviors Over Time In Children With Adverse Childhood Experiences: Focusing On The Role Of School Connectedness, Sei-Young Lee, Mijin Choi

Faculty Publications

Previous research has found the link between adverse childhood experiences (ACEs) and externalizing behaviors later in adolescence and adulthood. However, limited studies have explored longitudinal trajectories of aggressive behaviors affected by ACEs. This study aimed to investigate how aggressive behaviors change over time and compare the trajectories of aggressive behaviors between children with three or fewer (low-risk ACEs) and those with four or more ACEs (high-risk ACEs) with the role of school connectedness on the trajectories of aggressive behaviors over time. The study sample consisted of 4231 children collected by the Future of Families and Child Wellbeing Study, a longitudinal …

Implications Of Neural Compression To Scientific Images, João Phillipe Cardenuto, Joshua Krinsky, Lucas Nogueira, Aparna Bharati, Daniel Moreira

Computer Science: Faculty Publications and Other Works

While neural compression has the potential to revolutionize image compression, recent studies have emphasized its ability to introduce subtle artifacts that could alter the image content. Concerned about the impact of such modifications on scientific images, this work explores the potential effects of neural compression on these images, focusing on two critical aspects: semantic understanding and forensic integrity. We use scientific image datasets to assess the performance of neural compression techniques on Visual Question Answering (VQA) and copy-move forgery detection tasks. Our findings indicate that the subtle changes introduced by neural ] compression do not significantly degrade the performance of …

Dosimetric Advantage Of Scanning Beam Proton Therapy In Gynecologic Patients Receiving Adjuvant Radiotherapy, Rachel B Ger, Jarrod M Lentz, Joshua S Niedzielski, Sujay A Vora, Martin Bues, Danairis Hernandez Morales, Justin D Anderson, Christopher J Kutyreff, Christie A Schulz, Pedro R Lara, Ana K Ridgway, Pamela R Lemish, Justin D Gagneur, Aman Anand

Faculty, Staff and Student Publications

Background/Objectives: Adjuvant radiation for gynecologic malignancies often exposes organs at risk (OARs), such as the bone marrow, bowel, rectum, and bladder, to radiation, leading to toxicities that impact treatment tolerance and patient quality of life. Scanning proton beam therapy, particularly with Individual Field Simultaneous Optimization (IFSO), may offer dosimetric and biological advantages over volumetric modulated arc therapy (VMAT). This study evaluates the clinical impact of IFSO-based proton planning in post-operative gynecologic cancer patients.

Materials and Methods: Fourteen patients receiving adjuvant proton therapy to 45 Gy in 25 fractions were retrospectively analyzed. Comparison VMAT plans were generated on the same datasets. …

2025-06-17 Minutes, Morehead State University. Staff Congress.

Staff Congress Records

Staff Congress meeting minutes from June 17, 2025.

Aso Visual Abstract: Characterization Of The Germline Pathogenic Mutational Landscape And Oncologic Outcomes Among 877 Patients With Invasive Lobular Carcinoma, Victoria D Huynh, Jason Mouabbi, Henry M Kuerer, Kerollos Nashat Wanis, Hiam M Abdel-Salam, Angelica M Gutierrez, Helen M Johnson, Anthony Lucci, Kelly K Hunt, Banu K Arun

Faculty, Staff and Student Publications

No abstract provided.

Exploring Facilitators And Barriers Of Medically Tailored Meal Interventions For Patients Hospitalized With Acute Heart Failure: A Qualitative Study, Ihab Hassanieh, Sang Gune K Yoo, Jing Li, Virginia Mckay, Mark D Huffman

2020-Current year OA Pubs

BACKGROUND: Acute heart failure (AHF) is associated with high rehospitalization, morbidity, and mortality rates. Implementing medically tailored meal (MTM) interventions may help to optimize nutrition and improve outcomes for patients with AHF. This study aimed to explore facilitators and barriers to implementing an MTM intervention in a tertiary hospital.

METHODS: From August 2023 to October 2023, we conducted semistructured interviews with health care workers and patients with AHF at Barnes Jewish Hospital in Saint Louis, Missouri. The study used the updated Consolidated Framework for Implementation Research 2.0 to assess 5 domains: MTM characteristics, inner and outer settings, individual characteristics, and …

Appeal No.1032: K. Petroeum, Inc V. Division Of Oil & Gas Resources Management, Ohio Oil & Gas Commission

Ohio Oil & Gas Commission Decisions

No abstract provided.

Advances In Molecular Imaging For Neuroendocrine Neoplasms, Bradley Girod, Vikas Prasad

2020-Current year OA Pubs

Neuroendocrine neoplasms (NENs) represent a heterogenous group of tumors with significant inter- and intra-patient variability. Once considered to be rare, neuroendocrine neoplasms are being increasingly recognized through the advent of advanced diagnostic techniques, which may be contributing to the significant increase in the incidence and detection rate of these tumors. NENs can be classified into well differentiated and poorly differentiated neuroendocrine tumors (NETs) or neuroendocrine carcinomas (NECs). The proliferation rate of NETs can vary from Ki-67 1-55%. In addition, the SSTR expression can vary significantly. Because of this high "heterogeneity", their detection and characterization have become essential to disease management, …

Ldl Receptor-Mediated Endocytosis Of Escherichia Coli Α-Hemolysin Mediates Renal Epithelial Toxicity, Hunter W Kuhn, Madeleine R Smither, Rachel J Jin, Christina A Collins, Hongming Ma, Jason Sina, Joseph P Gaut, Michael S Diamond, David A Hunstad

2020-Current year OA Pubs

The α-hemolysin (HlyA) of uropathogenic

Effectiveness Of Digital Behavioral Activation Interventions For Depression And Anxiety: Systematic Review And Meta-Analysis, Eric Jia, Jushawn Macon, Michelle Doering, Joanna Abraham

2020-Current year OA Pubs

BACKGROUND: As digital interventions gain prominence in mental health care, they present opportunities to improve access and scalability. Despite their potential, the overall impact of digital behavioral activation (BA) interventions across different formats and populations is not yet fully understood.

OBJECTIVE: This systematic review examines the characteristics and functions of digital BA interventions and evaluates their effectiveness for mental health and other patient-related outcomes.

METHODS: A comprehensive search of databases (PubMed, Embase, Web of Science, APA PsycInfo, and ClinicalTrials.gov) was performed in November 2023 to identify randomized controlled trials (RCTs) assessing the effectiveness of digital BA interventions for depression and …

Atpase Level In Iraqi Diabetic Patients With And Without Diabetic Kidney Disease, Reham Khuldon Ibrahim, Kadhim K. Ghudhaib, Ali Abdulmajid Dyab Allawi

Baghdad Science Journal

Complications of diabetes have become one of the main problems that diabetics suffer from, especially during long periods. One of these complications is diabetic nephropathy, as both glomeruli and tubules are exposed to fibrosis due to diabetes. Recent years have witnessed great interest by researchers regarding diabetic nephropathy. This study aimed to determine the level of ATPase and some of the relevant biochemical factors in patients with diabetes and diabetic nephropathy compared with healthy controls. The study included 120 male and female (60 male and 60 female) ranging in age (30-65) years old. Ninety patients with type 2 diabetes are …

Synthesis And Biological Study Of Schiff–Mannich Base Derived From 2-Mercaptobenzimidazole As Chelated Tridentate Ligand With Some Transition Metal Ions Complexes, Thuraya Q. Sabah, Shaymaa R. Baqer

Baghdad Science Journal

To prepare a new ligand, many compounds were used to synthesize Schiff-Mannich base, such as isatin, Para chloro Aniline, 2-mercaptobenzimidazole and indole. The resulting compound 1-((2-((1H-indol-1-ylthio)-1H-benzo[d]imidazol-1-yl)methyl)-3-(4-chlorophenylimino)indolin-2-one (L). (L) was used to create a series of metal ion complexes with Co (II), Ni (II), Cu (II), Pd (II), Pt (IV), and Au (III). C.H.N.S., FTIR, mass spectra UV-ViS, 1H-NMR, 13CNMR, magnetic moment, and molar conductivity were used to characterize all of these compounds. Except for the palladium(II) and gold(III) complexes, all of the produced complexes had an octahedral geometry, according to the data. The antibacterial activity of the produced compounds was …

Synthesis, Characterization And Studying Mass Spectra And Thermal Analysis For Complexes Of Some Metal Ions And Determining Their Activity As Antioxidants, Fatimah Al-Zahraa Sh. H, Abbas Ali Salih Al-Hamdani

Baghdad Science Journal

The new azo dye was prepared from the reaction of the diazonium salt derived from 3-aminophenol with 2-hydroxyquinoline, then it was used to prepare a series of complexes with the chlorides of cobalt, chromium, copper, nickel, platinum, palladium and ammonium molybdate. The ligand was identified by a proton and carbon nuclear magnetic resonance spectroscopy, and the compounds were collected. The prepared materials were subjected to infrared, ultraviolet-visible, and mass spectrometry, as well as thermogravimetric analysis, differential calorimetry, and elemental analysis. Conductivity, magnetic susceptibility, metal content, and chlorine content of the complexes were also measured. The results showed that the ligand …

Synthesis, Antibacterial Evaluation, And Prediction Of The Ability Of Corrosion Inhibition By Density Functional Theory Of Azo-Dyes Of Folic Acid, Athra G. Sager, Jawad Kadhim Abaies, Aseel F. Abdullah

Baghdad Science Journal

In this study, new folic acid (B9) derivatives (a–d) were synthesized by reacting diazonium salt (of folic acid) with different aromatic compounds including 1-naphthol, 2-naphthol, pyrocatechol, or resorcinol. One of Schiff bases (I) was prepared by reacting folic acid with 4-amino benzaldehyde. Then, azo compound (e) was synthesized by coupling Schiff base with 1-naphthol. The new azo compounds (a–e) were identified by FTIR, ¹HNMR spectroscopy, and by measurements of some physical properties. The current work investigated the physical characteristics of compounds (a–e) in order to select the best corrosion inhibitor. 6-311G⁺/(d, p) base set and Density Functional …

Using Patient-Specific 3d-Printed C1-C2 Interfacet Spacers For The Treatment Of Type 1 Basilar Invagination: A Clinical Case Report, Tim T Bui, Alexander T Yahanda, Karan Joseph, Miguel Ruiz-Cardozo, Bernardo A De Monaco, Alexander Perdomo-Pantoja, Joshua P Koleske, Sean D Mcevoy, Camilo A Molina

2020-Current year OA Pubs

No abstract provided.

Sars-Cov-2 Nsp15 Enhances Viral Virulence By Subverting Host Antiviral Defenses, Allen Caobi, Tamarand L Darling, Adrianus C M Boon, Et Al.

2020-Current year OA Pubs

SARS-CoV-2 encodes numerous virulence factors, yet their precise mechanisms of action remain unknown. We provide evidence that the SARS-CoV-2 nonstructural protein 15 (nsp15) enhances viral virulence by suppressing the production of viral double-stranded (dsRNA), a potent inducer of antiviral signaling. The viral variants lacking nsp15 endoribonuclease activity elicited higher innate immune responses and exhibited reduced replication in human stem cell-derived lung alveolar type II epithelial cells, as well as in the lungs of infected hamsters. Consistently, these variants caused significantly less weight loss and mortality compared to wild-type (WT) virus in K18-hACE2 mice. Mechanistically, the cells infected with nsp15 mutants …