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Identifying Functional Regional Changes In Patients With Parkinson’S Disease And Freezing Of Gait, Taylor Robin Davis Sep 2025

Identifying Functional Regional Changes In Patients With Parkinson’S Disease And Freezing Of Gait, Taylor Robin Davis

All ETDs from UAB

Freezing of gait (FOG) affects anywhere from 20-80% of those with Parkinson’s Disease (PD) with the prevalence increasing with disease severity and length. This detrimental symptom is unpredictable and poorly characterized, which adds to the negative experience of FOG as it is difficult to treat. Using resting-state fMRI (rs-fMRI), we set out to determine the functional changes in those with PD-FOG and map onto regions of the brain where signal power, measured using fractional amplitude of low-frequency fluctuations (fALFF), synchronicity, as measured by regional homogeneity (ReHo), and strength and direction of intra-network connections as measured by static effective connectivity, are …


Understanding Visual Memory Encoding And The Effects Of Endurance Exercise In Healthy Adults And People With Temporal Lobe Epilepsy, Brandon Steve Mitchell Sep 2025

Understanding Visual Memory Encoding And The Effects Of Endurance Exercise In Healthy Adults And People With Temporal Lobe Epilepsy, Brandon Steve Mitchell

All ETDs from UAB

The visual memory encoding (VME) of indoor and outdoor scenes is a complex cognitive process that primarily utilizes the bilateral hippocampi and medial temporal lobe (MTL) structures along the parahippocampal gyrus. Alpha and theta frequency band oscillations are important in the processing of visual information and memory formation, respectively. We completed hree studies investigating the cognitive process of VME in healthy adults and people with temporal lobe epilepsy (pwTLE). The first study was cross-sectional and utilized a functional magnetic resonance imaging (fMRI)-constrained magnetoencephalography (MEG) analysis in healthy adults to explore VME. The fMRI results were used as spatial priors to …


Wellness Perspectives Of Early Career Teachers: An Exploratory Study Of School Culture And Environment, Amy Day Carr Sep 2025

Wellness Perspectives Of Early Career Teachers: An Exploratory Study Of School Culture And Environment, Amy Day Carr

All ETDs from UAB

Purpose: The purpose of this grounded theory dissertation was to explore Alabama early career teachers’ perceptions of personal wellness, the wellness culture and environment of their school system, and the impact of the culture on teacher wellness and job satisfaction. Early career teachers face unique challenges that contribute to high turnover rates, yet their wellness has not been systematically studied. This study sought to answer three primary research questions with sub-questions. Teachers were asked to describe their personal wellness, the wellness culture at their schools, and the effect of the wellness culture and environment of the school on teachers in …


Measuring Emotional Well-Being Across Neurodevelopmental Disorders, Alecia Mercier Sep 2025

Measuring Emotional Well-Being Across Neurodevelopmental Disorders, Alecia Mercier

All ETDs from UAB

Emotional well-being has emerged as a crucial determinant of both physical and mental health, with implications for public health initiatives aimed at improving quality of life and reducing mortality. However, a clear and consistent understanding of emotional well-being, its components, and optimal measurement strategies remains lacking, especially for individuals with neurodevelopmental disorders. This dissertation investigated how emotional well-being is conceptualized and measured in individuals with neurodevelopmental disorders. Paper one was a scoping review that identified 20 emotional well-being measures developed or adapted for individuals with intellectual and developmental disabilities. Findings demonstrated that many existing measures do not include emotional well-being …


Strength Of Self-Adhesive Resin Cements To Shortened Zirconia Crowns With And Without An Additional Coat Of Adhesive, Abraham John Sep 2025

Strength Of Self-Adhesive Resin Cements To Shortened Zirconia Crowns With And Without An Additional Coat Of Adhesive, Abraham John

All ETDs from UAB

Self-adhesive cements have gained popularity due to their ease of manipulation as they do not require a separate etch, primer, or bonding agent to bond to the tooth or crown surfaces. However, the use of an adhesive bonding agent with a self-adhesive resin cement has been claimed to improve its bond strength. The objective of this study is to compare the retention bond strength of 2 self-adhesive cements on to the tooth surface with and without the use of bonding agents with a known control. Materials and Methods: A total of 50 extracted non-carious second premolar teeth were collected. The …


Bayesian Mediation Analyses For Zero-Inflated Data, Jinhong Cui Sep 2025

Bayesian Mediation Analyses For Zero-Inflated Data, Jinhong Cui

All ETDs from UAB

Mediation analysis is a fundamental tool in causal inference, enabling researchers to disentangle the pathways through which an exposure affects an outcome via intermediate variables (mediators). It has been widely applied in social sciences, psychology, and healthcare-related studies. Traditionally, mediation analysis has been conducted within the linear structural equation modeling (LSEM) framework. However, recent advances have shifted attention toward the counterfactual framework due to its flexibility in defining causal effects and its capacity to accommodate non-linear relationships, parametric and non-parametric models. Building on this foundation, methodological developments in mediation analysis have expanded to address more complex data structures, such as …


Those Who Strayed: Quaker Abolitionist John Greenleaf Whittier, And The Grimké Sisters Who Broke From The Society Of Friends To Actively Oppose Slavery In The Antebellum Era, David Mark Ledlow Sep 2025

Those Who Strayed: Quaker Abolitionist John Greenleaf Whittier, And The Grimké Sisters Who Broke From The Society Of Friends To Actively Oppose Slavery In The Antebellum Era, David Mark Ledlow

All ETDs from UAB

The adoption of quietist theology by the Quaker community in the early nineteenth century created a divide between Quakers and their abolitionist movement due to the differing methods to achieve an end to slavery. Quaker society began to adopt a stance that avoided all controversies and employed conservative actions to combat slavery while abolitionist began to speak out more against the evils of slavery regardless of any controversy. This divided left Quaker abolitionist like John Greenleaf Whittier and the Grimké sisters with the choice to distance themselves from the Society of Friends or the abolitionist movement. Whittier and the Grimké …


Reducing The Risk Of Phishing Attacks Through Providing Consistent Training Via The Kaizen Education Platform, Dalton E. Pena Sep 2025

Reducing The Risk Of Phishing Attacks Through Providing Consistent Training Via The Kaizen Education Platform, Dalton E. Pena

All ETDs from UAB

Introduction: Many healthcare organizations have experienced cybersecurity related phishing email attacks, costing these organizations many thousands to many millions of dollars to release ransomed data to maintain patient care delivery and operations. Use of technology alone has proven to be insufficient, as most occurrences of data breaches are related to human behaviors. Background: Cybersecurity is a threat increasingly common to healthcare organizations, and it is gaining in both sophistication and complexity, aided by artificial intelligence. Healthcare organizations have adopted new technologies and increased the prevalence of cybersecurity training to develop a healthy cyber culture and construct a human firewall against …


Influence Of Transportation Access And Geographic Distance On Retention And Completion In Substance Use Treatment: A Retrospective Study Of In-Person And Telehealth Modalities, Yasemin Tanik Morgan Sep 2025

Influence Of Transportation Access And Geographic Distance On Retention And Completion In Substance Use Treatment: A Retrospective Study Of In-Person And Telehealth Modalities, Yasemin Tanik Morgan

All ETDs from UAB

Substance use disorder (SUD) remains a public health concern in the United States. Low-income and underserved populations continue to face barriers to accessing effective treatment. The rapid expansion of telehealth has been proposed as a solution to barriers such as geographic distance and transportation. However, it is unclear whether telehealth effectively addresses these barriers or how access-related factors influence treatment outcomes across care modalities. This retrospective cohort study, guided by the Social Determinants of Health (SDOH) framework, evaluated the impact of treatment modality, transportation access, and geographic distance on retention and completion in a publicly funded Intensive Outpatient Program (IOP) …


Psychedelics And Psychosis: An Interdisciplinary Re-Examination, Haley Maria Dourron Sep 2025

Psychedelics And Psychosis: An Interdisciplinary Re-Examination, Haley Maria Dourron

All ETDs from UAB

Psychedelics have long been described as potential drug models of psychosis. There is also concern that psychedelics might worsen psychotic symptoms in individuals with a history of psychotic disorders. This dissertation examines the overlap between psychedelic-induced states and non-affective psychotic disorders (NAPD) from both subjective and neurological perspectives, as well as the perceived impact of psychedelic use in people with a history of NAPD. Nineteen people with a history of NAPD who had used psychedelics were interviewed about their experiences. Participants generally described psychedelic and psychotic experiences as distinct, though both affected similar experiential domains, such as sensory alterations. Altered …


Policy Surveillance Of Decommissioning And Decontamination Regulations, Joshua Gary Collins Sep 2025

Policy Surveillance Of Decommissioning And Decontamination Regulations, Joshua Gary Collins

All ETDs from UAB

Policy surveillance is an analytical collection and study of regulatory action within the public health field. Due to the vastness of the United States regulatory system, large amounts of time and effort are needed for policy surveillance to be accomplished. When considering nuclear decommissioning policies, all state and local regulations should start with the Nuclear Regulatory Commission’s (NRC) requirements, however states have the freedom to increase stringency. Policy surveillance allows for both a convenient and approachable way to reference important regulations in a timely manner while also functioning as a method to identify and manage any inconsistencies found between the …


Murder On The Axonal Express: Synucleinopathy Disruption Of 14-3-3 Protein-Protein Interactions Impairs Axonal Trafficking, Frank Sanders Pair Sep 2025

Murder On The Axonal Express: Synucleinopathy Disruption Of 14-3-3 Protein-Protein Interactions Impairs Axonal Trafficking, Frank Sanders Pair

All ETDs from UAB

14-3-3s are a family of proteins highly expressed in the brain. These proteins exert their functions mainly through protein-protein interactions (PPIs) and have thousands of binding partners. 14-3-3s have been shown to be present in pro-teinaceous aggregates of alpha-synuclein (αsyn), a pathological hallmark of synucleinopathies. This includes such diseases as Parkinson’s Disease (PD) and Dementia with Lewy Bodies (DLB). 14-3-3 proteins, particularly the 14-3-3θ isoform, have been shown to be protective in multiple synucleinopathy models. However, reduced soluble levels of 14-3-3s and increased phosphorylation of 14-3-3θ at S232 observed in synucleinopathies like DLB may impair their protec-tive ability, potentially due …


In-Vitro Antimicrobial Activity, Demineralization Inhibition Potential, And Staining Effects Of Three Silver Fluoride Materials, Quiesly Torres Sep 2025

In-Vitro Antimicrobial Activity, Demineralization Inhibition Potential, And Staining Effects Of Three Silver Fluoride Materials, Quiesly Torres

All ETDs from UAB

Background: Dental caries is a worldwide preventable public health problem. It affects any individuals regardless of their gender, age, ethnicity, and socioeconomic status. Silver diamine fluoride (SDF) has been extensively used for preventing cavities, but it has side effects; therefore, some other biocompatible antimicrobial and remineralization-inducing alternative materials have been developed. Purpose: This study aims to assess the in vitro antimicrobial activity, demineralization inhibition potential, and staining effect of SDF, Nano Silver Fluoride (NSF), and silver fluoride + potassium iodide (SF/KI). Methods: Three silver diamine fluoride-based materials were tested: SDF 38% (Advantage Arrest®, Elevate Oral Care, USA), 1500 ppm NSF …


Attitudes Of Optometrists Towards Referring Patients For Nutrition And Lifestyle Changes To Treat Diabetic Retinopathy And Age-Related Macular Degeneration, Samantha Chapman Sep 2025

Attitudes Of Optometrists Towards Referring Patients For Nutrition And Lifestyle Changes To Treat Diabetic Retinopathy And Age-Related Macular Degeneration, Samantha Chapman

All ETDs from UAB

Some ocular diseases, including diabetic retinopathy and age-related macular degeneration, have direct links to systemic diseases or the condition of the body as a whole. Specific diet and lifestyle changes have been studied in relation to these two diseases and, while it is not within the scope of optometry to prescribe these changes for patients, an optometrist may refer to a variety of specialists so that patients can receive the most holistic care available. The primary goal of this study is to assess the attitudes of currently practicing United States optometrists towards referring to nutrition and lifestyle professionals and their …


Core Body Temperature (Cbt) Monitoring Among Groundskeepers Under Heat Stress, Lara Anne Parker Sep 2025

Core Body Temperature (Cbt) Monitoring Among Groundskeepers Under Heat Stress, Lara Anne Parker

All ETDs from UAB

Heat is the leading cause of death for outdoor workers. In Alabama, there are an estimated 11,160 - 21,460 workers employed in the landscaping and groundskeeping industry, which are vulnerable to heat-related illnesses (HRI). In this study, core body temperature (CBT) and wet-bulb globe temperature (WBGT) index were examined as potential indicators of heat stress. A SlateSafety (SS) Band V2 wearable CBT device and a WBGT monitor were used to collect CBT and WBGT values, respectively. Sampling for the main two measurements was performed over five days for two participants. An additional CBT device option, the e-Celsius performance ingestible pill, …


High Pressure And High Temperature Exploration Of Phase Transformations In Eutectic High Entropy Alloys, Andrew Dobbs Pope Sep 2025

High Pressure And High Temperature Exploration Of Phase Transformations In Eutectic High Entropy Alloys, Andrew Dobbs Pope

All ETDs from UAB

The overall goal of this research is to understand the mechanism of phase transformations in far-from-equilibrium micro/nano-structures produced by additive manufacturing of compositionally complex alloys. The eutectic high entropy alloys based on nickel were selected for this study because of their high tensile strength as well as high ductility. This study examines the high-pressure behavior and phase transformations of laser powder-bed fusion manufactured eutectic high-entropy alloys, specifically AlCoCrFeNi2.1 and Al18Co20Cr10Fe10Ni40W2. Both alloys exhibit a nano-lamellar structure composed of body-centered cubic and face-centered cubic phases. The BCC to FCC phase transformation under high pressures was observed below 21 Giga-Pascals (GPa) in …


Voices From Two Secondary Newcomer Programs In Alabama: A Multiple Case Study, Carl Anthony Floyd Jr Sep 2025

Voices From Two Secondary Newcomer Programs In Alabama: A Multiple Case Study, Carl Anthony Floyd Jr

All ETDs from UAB

This multiple case study investigates the design, implementation, and impact of two secondary newcomer programs in Alabama, with a focus on Students with Limited or Interrupted Formal Education (SLIFE) and non-SLIFE English Learner (EL) newcomers. Grounded in Esteban-Guitart et al.’s (2023) framework of community funds of knowledge and identity, the study explores how these programs function as community educational ecosystems. Data were collected through interviews, observations, and document reviews across two school districts and analyzed using ethnographic and thematic methods. Findings reveal that when newcomer programs are intentionally designed to leverage community assets and cultural identities, they provide meaningful academic, …


Origin And Differentiation Of Uterine Natural Killer Cells, Morgan Elizabeth-Carolyn Greene Sep 2025

Origin And Differentiation Of Uterine Natural Killer Cells, Morgan Elizabeth-Carolyn Greene

All ETDs from UAB

Uterine natural killer (uNK) cells are specialized tissue-resident lymphocytes that are essential for successful pregnancy, yet the molecular pathways governing their development remain poorly defined. This knowledge gap hinders our ability to understand and treat immunological complications of pregnancy, such as recurrent pregnancy loss and pre-eclampsia. This dissertation addresses the fundamental question of how human uNK cells arise and differentiate, first within the non-pregnant endometrium and subsequently within the decidua of early pregnancy. Using single-cell RNA sequencing of matched human blood and endometrial samples, we first delineate the developmental trajectory of endometrial NK (eNK) cells. We demonstrate that eNK cells …


Evaluating Relative Value Units And Its Alternatives Inradiology, Alfred Matthew Dewey Sep 2025

Evaluating Relative Value Units And Its Alternatives Inradiology, Alfred Matthew Dewey

All ETDs from UAB

ABSTRACT Radiologist productivity in the United States is traditionally measured by thework Relative Value Unit (wRVU). However, wRVUs fail to capture several criticalaspects of radiologic work, including study complexity, patient characteristics, andfacility factors. This study evaluated the statistical significance of wRVUs as a measureof productivity and proposes an alternative model grounded in ascribable time—themeasurable duration a radiologist takes to interpret and report a study.A comprehensive literature review highlighted the inadequacy of wRVUs inaccounting for technological advances, case complexity, and workflow differences.Applying contingency theory as the guiding framework, this study posited that radiologyproductivity must be tailored to institutional and contextual variability. …


The Impact Of Nursing Home Ownership On The Election Of Advance Care Directives, Natasha Elie Sep 2025

The Impact Of Nursing Home Ownership On The Election Of Advance Care Directives, Natasha Elie

All ETDs from UAB

Approximately 1.3 million people reside in nursing homes/facilities. Annually, this population represents 25% of mortalities in the United States. The enactment of the Patient Self Determination Act (PSDA) required nursing homes/facilities to educate patients, staff, and the community on the right to advance care directives. This study aimed to evaluate the impact of nursing home ownership on the election of ACDs and end-of-life care services by examining how nursing homes ownership in New York, California, and Ohio compared regarding residents' election of ACD and whether there was a variation in the these states. This paper contributes to the literature by …


An Analysis Of Gamma-Aminobutyric Acid Levels With Structural And Functional Connectivity In Antipsychotic Medication-Naïve First Episode Psychosis Patients, Genelle Dano Samson Sep 2025

An Analysis Of Gamma-Aminobutyric Acid Levels With Structural And Functional Connectivity In Antipsychotic Medication-Naïve First Episode Psychosis Patients, Genelle Dano Samson

All ETDs from UAB

Schizophrenia is a complex psychiatric disorder characterized by hallucinations, delusions, disorganized thinking, and abnormalities in emotional expression, social interaction, and motivation. Cognitive deficits and alterations in perception are core features of psychosis spectrum disorders, though the neurobiological bases of these impairments are not thoroughly understood. Examination of the structures responsible for cognition, along with how their connectivity and neurochemistry are altered in psychosis, is crucial to understanding and treating schizophrenia. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system and plays a crucial role in the regulation of neuronal activity. Evidence from animal models and postmortem …


2025 September 11 - Tennessee Weekly Drought Summary, Tennessee Climate Office, East Tennessee State University Sep 2025

2025 September 11 - Tennessee Weekly Drought Summary, Tennessee Climate Office, East Tennessee State University

Tennessee Climate Office Weekly Drought Summaries

No abstract provided.


Mechanistic Rationale For Toxic Aerosol Formation From Vaping And Dabbing With A Focus On Ketene Formation From Semi-Synthetic Cannabinoids, Kaelas Ryan Munger Sep 2025

Mechanistic Rationale For Toxic Aerosol Formation From Vaping And Dabbing With A Focus On Ketene Formation From Semi-Synthetic Cannabinoids, Kaelas Ryan Munger

Dissertations and Theses

Cannabis vaping is a relatively new route of administration that is increasingly common among youth and young adults. Cannabis vaping has been associated with cases of acute lung injury, especially among youth, and most often involves the heating and aerosolization of high-potency products having cannabinoid concentrations of ~ 50 % - 98 % w/w. Proponents argue that vaping is safer than smoking due to the characteristically lower temperatures and fewer plant materials. However, the extreme cannabinoid concentrations, along with non-natural formulations having undisclosed additives, require studies of the health effects of these products. Despite the popularity of cannabis concentrate vaping, …


From Model To Behavior: Methodological Challenges In Using Fuzzy Cognitive Maps To Represent Mental Models, Catherine Elizabeth Moore Sep 2025

From Model To Behavior: Methodological Challenges In Using Fuzzy Cognitive Maps To Represent Mental Models, Catherine Elizabeth Moore

Dissertations and Theses

This dissertation examines the use of Fuzzy cognitive maps (FCMs) as representations of mental models and how these representations connect to individual behavior. Fuzzy cognitive maps (FCMs) are semi-quantitative models that encode cause-and-effect structures as directed graphs. They are frequently used to represent individuals' knowledge structures/mental models and should in theory correlate to actions and decisions of individuals; however, this correlation has yet to be explored in a systematic manner. This dissertation represents the first step in experimental research on the connection between FCMs and individual behavior. It examines two theories of how humans use mental models, one based on …


Spartan Daily, September 11, 2025, San Jose State University, School Of Journalism And Mass Communications Sep 2025

Spartan Daily, September 11, 2025, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2025

Volume 165, Issue 9


The George-Anne Daily, Georgia Southern University Sep 2025

The George-Anne Daily, Georgia Southern University

George-Anne Media Group: Newsletters & Magazines

No abstract provided.


Nmr-Based Metabolomic Profiling For Brain Cancer Diagnosis And Treatment Guidance, Julia R Zickus, José S Enriquez, Paytience Smith, Bill T Sun, Muxin Wang, Aldo Morales, Pratip K Bhattacharya, Shivanand Pudakalakatti Sep 2025

Nmr-Based Metabolomic Profiling For Brain Cancer Diagnosis And Treatment Guidance, Julia R Zickus, José S Enriquez, Paytience Smith, Bill T Sun, Muxin Wang, Aldo Morales, Pratip K Bhattacharya, Shivanand Pudakalakatti

Faculty, Staff and Student Publications

Nuclear magnetic resonance (NMR) spectroscopy is a routinely used analytical tool for studying chemical entities of varying molecular sizes, ranging from approximately 20 Da to ~45 kDa, and in some cases even larger. Over the past two decades, the use of NMR spectroscopy has significantly expanded to the study of metabolomics. In this medium-sized review, the application of NMR-based metabolomics in the diagnosis, therapeutic intervention, and guidance of therapy for various types of brain cancer is discussed.


Ubiquitination-Targeted Therapies Improve Bmd Ipsc Myogenic Cell Engraftment And Dystrophin Expression In Vivo, Muchen Liu, Somik Chatterjee, Jianbo Wu, Zeinab Kashaniasl, Ashok Kumar, Chunru Lin, Radbod Darabi Sep 2025

Ubiquitination-Targeted Therapies Improve Bmd Ipsc Myogenic Cell Engraftment And Dystrophin Expression In Vivo, Muchen Liu, Somik Chatterjee, Jianbo Wu, Zeinab Kashaniasl, Ashok Kumar, Chunru Lin, Radbod Darabi

Faculty, Staff and Student Publications

Becker muscular dystrophy (BMD) is caused by in-frame mutations in dystrophin gene, leading to progressive muscle weakness, and cardiac and respiratory complications. Currently, there is no cure. We have recently identified the importance of poly-ubiquitination in regulating dystrophin stability through the binding of lncRNA H19 to the dystrophin C-terminal zinc-finger domain (ZNF), inhibiting TRIM63-mediated poly-ubiquitination. We also demonstrated that BMD mutations lead to conformational changes in ZNF domain, reduced lncRNA H19 binding and increased dystrophin ubiquitination. Here we used BMD iPSCs to investigate the in vitro myogenic potential of BMD myogenic cells, as well as in vitro and in vivo …


Investigating Temperature Influences On Shell Growth And Microstructural Variations In Bay Scallops: Insights From Multiscale Microscopy, Benazir Khurshid, Arnaud Benchetrite, Lise Guichaoua, Nicolas Brodusch, Bryce D. Stewart, Roland Kröger, Raynald Gauvin, Martin Mallet, Réjean Tremblay, Natalie Reznikov Sep 2025

Investigating Temperature Influences On Shell Growth And Microstructural Variations In Bay Scallops: Insights From Multiscale Microscopy, Benazir Khurshid, Arnaud Benchetrite, Lise Guichaoua, Nicolas Brodusch, Bryce D. Stewart, Roland Kröger, Raynald Gauvin, Martin Mallet, Réjean Tremblay, Natalie Reznikov

School of Biological and Marine Sciences

Scallops (order Pectinida) are well-known for their robust and beautiful calcitic shells that protect them from external impacts and predators. Scallops respond to environmental conditions, including water temperature, salinity, and food supply, which are reflected in the shell growth rates and patterning. The bay scallop (Argopecten irradians) is a species of high ecological and aquacultural value in North America, and its habitat may expand towards higher latitudes with inevitable global warming. To investigate the effect of water temperature on the accretion rate and the polycrystalline microstructure of the Bay scallop shell, we conducted a controlled growth experiment on juveniles, 4 …


Combined Crystallographic Study Of King Scallop (Pecten Maximus) Shells Using Sem, Ebsd And Raman Spectroscopy, Lise Guichaoua, Natalie Reznikov, Bryce D. Stewart, Roland Kröger, Raynald Gauvin Sep 2025

Combined Crystallographic Study Of King Scallop (Pecten Maximus) Shells Using Sem, Ebsd And Raman Spectroscopy, Lise Guichaoua, Natalie Reznikov, Bryce D. Stewart, Roland Kröger, Raynald Gauvin

School of Biological and Marine Sciences

The shells of Pecten maximus (king scallop) are composed primarily of polycrystalline calcitic calcium carbonate, with a crucial aragonitic prismatic myostracum layer that facilitates soft tissue attachment and contributes to mechanical strength. Despite its importance, the impact of environmental stressors, such as metal contamination, on the myostracum remains underexplored. Hence, this study's main goal was to shed light on the microstructure and crystallography of king scallop shells, particularly the myostracum region, using a combination of scanning electron microscopy (SEM), electron backscatter diffraction (EBSD) analysis, and Raman spectroscopy. This approach was chosen to develop new protocols that integrate imaging techniques for …