Digital Commons Network^™

The Efficacy Of Longevity Interventions In Caenorhabditis Elegans Is Determined By The Early Life Activity Of Rna Splicing Factors, Sneha Dutta, Maria Camila Perez Matos, Caroline Heintz, Ayse Sena Mutlu, Mary Piper, Meeta Mistry, Arpit Sharma, Christopher S Morrow, Hannah Smith, Porsha Howell, Rohan Sehgal, Anne Lanjuin, Meng C Wang, William B Mair

Faculty, Staff and Students Publications

Geroscience aims to target the aging process to extend healthspan. However, even isogenic individuals show heterogeneity in natural aging rate and responsiveness to pro-longevity interventions, limiting translational potential. Using RNAseq analysis of young, isogenic, subpopulations of Caenorhabditis elegans selected solely on the basis of the splicing pattern of an in vivo minigene reporter that is predictive of future life expectancy, we find a strong correlation in young animals between predicted life span and alternative splicing of mRNAs related to lipid metabolism. The activity of two RNA splicing factors, Reversed Polarity-1 (REPO-1) and Splicing Factor 1 (SFA-1), early in life is …

Paediatric Difficult Intubations And The Impact Of Timing Of Weekend Versus Weekday Cases: An Analysis Of The Pediatric Difficult Intubation Registry, Christy J Crockett, Judit Szolnoki, Sabina Khan, Angela C Lee, Alexander Kaizer, Patrick Olomu, Melissa Brooks Peterson

Faculty, Staff and Students Publications

Background: Off-hours presentation has been shown to be independently associated with tracheal intubation-related adverse events in patients with predicted difficult airway. The comparison of the complication rates of paediatric difficult intubations on weekends vs weekdays is unknown. The primary objective of this retrospective review is to determine the complication rates for paediatric difficult intubation cases that occur on a weekend vs a weekday.

Methods: We performed a retrospective review of data from the Pediatric Difficult Intubation registry, with service dates June 8, 2012 to May 9, 2023, to compare complication rates (total complications, hypoxaemia, non-hypoxaemia complications, and first-attempt success rate) …

Bicontinuous Interconnected Porous Biomaterials For Tissue Engineering And Regeneration, Aihik Banerjee, Anjana Khanal, Prince D Okoro, Shankar P Kharal, Kevin Dalsania, Baishali Kanjilal, Shiril B Iragavarapu, Yiqing Chen, Janitha M Unagolla, Huinan H Liu, Joshua T Morgan, Robert P Hesketh, Arash Pezhouman, Reza Ardehali, Bahman Anvari, Martin F Haase, Iman Noshadi

Faculty, Staff and Students Publications

Biomaterials mimicking natural extracellular matrix are necessary to create an optimal microenvironment for cell adhesion, migration, proliferation, and differentiation. These scaffolds must possess bicontinuous interconnected porosity to ensure the effective exchange of oxygen, nutrients, and metabolic waste, which are crucial for developing functional tissues. Here, a novel bicontinuous interfacially jammed emulsion (BIJEL)-Integrated PORous Engineered System (BIPORES) is developed to confer bioinert synthetic polyethylene glycol diacrylate (PEGDA) with unique bicontinuous interconnected porosity and surface topography. This platform is fabricated through controlled phase separation and interfacial stabilization of two continuous phases by nanoparticles. Functional validation using human mesenchymal stem cells, and human …

High Risk Of Persistence And Risk Of Dysplasia After Diagnosis Of Ultrashort Barrett's Esophagus, Wasseem Skef, Jasmine Haydel, Ashwin Rao, Ronan Allencherril, Rollin George, Gyanprakash A Ketwaroo, Aaron P Thrift, Hashem B El-Serag, Theresa Nguyen Wenker

Faculty, Staff and Students Publications

Introduction: The management of ultrashort (< 1 cm) Barrett's esophagus (BE) remains unclear. We aimed to determine the prevalence of ultrashort BE (USBE) at index diagnosis, identify factors associated with persistent BE after USBE diagnosis, and identify risk of dysplasia after initial USBE in a population of US veterans.

Methods: This was a retrospective cohort study at the Veterans Affairs hospital in Houston, TX, of consecutive patients with new BE diagnosis from November 1990 to June 2022 with follow-up through April 2023. Using a pathology database, we identified patients with a new USBE diagnosis and any subsequent follow-up esophagogastroduodenoscopy (EGD). We examined the association of sociodemographic and clinical risk factors of persistent USBE cohort compared with longer-length segment BE and those with a negative follow-up EGD after index USBE with χ 2 tests and logistic regression models. Last, we calculated the prevalence and …

Ambulatory Acute Kidney Injury In Patients With Cirrhosis Is Common And Burdensome, Kavish R Patidar, Ruben Hernaez, Yan Liu, George Cholankeril, Jennifer R Kramer, Thom Taylor, Prasun Jalal, Tzu-Hao Lee, Avegail G Flores, Giuseppe Cullaro, Andrew S Allegretti, Pere Gines, Fasiha Kanwal

Faculty, Staff and Students Publications

Background & aims: Little is known about ambulatory acute kidney injury (A-AKI), which develops in an outpatient setting.

Methods: We conducted a retrospective cohort study of patients diagnosed with cirrhosis between January 1, 2018, and December 31, 2019 from 130 hospitals in the Veterans Affairs healthcare system. Patients were classified as having incident A-AKI if they met the International Club of Ascites AKI criteria in an outpatient setting. We used multivariable regression models to identify factors associated with A-AKI development within 1 year of cirrhosis diagnosis. We also conducted a structured implicit review of patients' medical charts to determine the …

Targeting Steroid Receptor Coactivators For The Treatment Of Benign Female Reproductive Disorders, Yuri Park, Jaeyeong Jeong, Eunsu Kim, Nuri Sung, Sang Jun Han

Faculty, Staff and Students Publications

Steroid receptor coactivators (SRCs) are master regulators of nuclear receptor signaling and play essential roles in female reproductive physiology. By integrating steroid hormone signaling with growth factors and metabolic pathways, SRC-1, SRC-2, and SRC-3 coordinate key processes such as decidualization, placental development, and ovarian function. Dysregulation of SRCs is strongly linked to the progression of benign gynecologic disorders, including polycystic ovary syndrome (PCOS), endometriosis, and uterine fibroids, largely through enhancing hormonal hypersensitivity and disrupting nuclear receptor-mediated cellular pathways. Emerging evidence further implicates specific SRC isoforms in disease pathogenesis, underscoring their potential as biomarkers and therapeutic targets. To inhibit SRC activity, …

Gregor: Accelerating Genomics For Rare Diseases, Moez Dawood, Ben Heavner, Marsha M Wheeler, Rachel A Ungar, Jonathan Lotempio, Laurens Wiel, Seth Berger, Jonathan A Bernstein, Jessica X Chong, Emmanuèle C Délot, Evan E Eichler, James R Lupski, Ali Shojaie, Michael E Talkowski, Alex H Wagner, Chia-Lin Wei, Christopher Wellington, Matthew T Wheeler, Gregor Partner Members, Claudia M B Carvalho, Richard A Gibbs, Casey A Gifford, Susanne May, Danny E Miller, Heidi L Rehm, Kaitlin E Samocha, Fritz J Sedlazeck, Eric Vilain, Anne O'Donnell-Luria, Jennifer E Posey, Lisa H Chadwick, Michael J Bamshad, Stephen B Montgomery, Genomics Research To Elucidate The Genetics Of Rare Diseases (Gregor) Consortium

Faculty, Staff and Students Publications

Rare diseases are collectively common, affecting approximately 1 in 20 individuals worldwide. In recent years, rapid progress has been made in rare disease diagnostics due to advances in next-generation sequencing, development of new computational and functional genomics approaches to prioritize genes and variants and increased global sharing of clinical and genetic data. However, more than half of individuals suspected to have a rare disease lack a genetic diagnosis. The Genomics Research to Elucidate the Genetics of Rare Diseases (GREGoR) Consortium was initiated to study thousands of challenging rare disease cases and families and apply, standardize and evaluate emerging genomics technologies …

Optic Nerve Injury Impairs Intrinsic Mechanisms Underlying Electrical Activity In A Resilient Retinal Ganglion Cell, Thomas E Zapadka, Nicholas M Tran, Jonathan B Demb

Faculty, Staff and Students Publications

Retinal ganglion cells (RGCs) are the sole output neurons of the retina and convey visual information to the brain via their axons in the optic nerve. Following injury to the optic nerve, RGCs axons degenerate and many cells die. For example, a model of axon injury, the optic nerve crush (ONC), kills ~80% of RGCs after two weeks. Surviving cells are biased towards ‘resilient’ types, including several with sustained firing to light stimulation. RGC survival may depend on activity, and there is limited understanding of how or why activity changes following optic nerve injury. Here we quantified the electrophysiological properties …

Neurodevelopmental Abnormalities Underlying Behavioral Deficits In A Model Of Pediatric Obstructive Sleep Apnea, Arvind Chandrakantan, Michael R Williamson, Vaishnav Krishnan, Mahyar J Hedayatpour, Adam C Adler, Nandani Adyapak, Chris S Ward, Russell Ray, David Durgan, Farrah Kheradmand, Benjamin Deneen

Faculty, Staff and Students Publications

Rationale: Pediatric Obstructive Sleep Apnea (POSA) is a relatively common childhood sleep disorder whose neurodevelopmental phenotype includes deficits in learning and memory, olfaction, and fine motor abilities.

Objectives: To date, there has not been a validated preclinical model of POSA, hampering efforts in understanding how nocturnal episodes of intermittent hypoxia disrupt neurodevelopmental trajectories. The objective of this study was to create a faithful sculpting of the human condition in a preclinical murine model.

Methods: We used clinical data from children with POSA to develop and validate a mouse model of POSA that faithfully recapitulates several behavioral deficits seen in the …

Effects Of Ultrasound-Assisted Extraction And Transglutaminase Treatment On The Physicochemical Properties Of Protein From Stropharia Rugosoannulata, Lingyu Yang, Lili Yang, Xi Feng, Yang Xiao, Xiaoju Tian, Wen Huang, Ying Liu

Faculty Research, Scholarly, and Creative Activity

Protein from edible mushrooms can serve as a new alternative protein to replace meat in various food formulations. However, the processing characteristics of mushroom protein were poor. In this study, ultrasound-assisted extraction (UAE) and transglutaminase (TGase) were used for the modification of protein from Stropharia rugosoannulata (SRP) to improve its utilization. The optimal parameters of UAE were 480 W for 20 min. The process of TGase modification was optimized by a single-factor experiment and response surface test to obtain the TGase dosage of 0.36 % (w/w), cross-linking at 40℃ for 1.5 h, and reaction pH of 5.4. The effects of …

Genome Sequencing Reveals The Impact Of Pseudoexons In Rare Genetic Disease, Georgia Pitsava, Megan Hawley, Light Auriga, Ivan De Dios, Arthur Ko, Sofia Marmolejos, Miguel Almalvez, Ingrid Chen, Kaylee Scozzaro, Jianhua Zhao, Rebekah Barrick, Nicholas Ah Mew, Vincent A Fusaro, Jonathan Lotempio, Matthew Taylor, Luisa Mestroni, Sharon Graw, Dianna Milewicz, Dongchuan Guo, David R Murdock, Kinga M Bujakowska, Changrui Xiao, Emmanuèle C Délot, Seth I Berger, Eric Vilain

Faculty, Staff and Student Publications

Purpose: Advancements in sequencing technologies have significantly improved clinical genetic testing; yet, the diagnostic yield remains around 30% to 40%. Emerging technologies are now being deployed to address the remaining diagnostic gap.

Methods: We tested whether short-read genome sequencing could increase the diagnostic yield in individuals enrolled into the UCI-GREGoR research study, who had suspected Mendelian conditions and prior inconclusive testing. Two other collaborative research cohorts, focused on aortopathy and dilated cardiomyopathy, consisted of individuals who were undiagnosed but had not undergone harmonized prior testing.

Results: We sequenced 353 families (754 participants) and found a molecular diagnosis in 54 (15.3%) …

Restricting Metabolic Plasticity Enhances Stress Adaptation Through The Modulation Of Pdh And Hif1a In Trap1-Depleted Colon Cancer, Hong-Yuan Tsai, Miao-Hsueh Chen, Jihye Yun, Lisa A Lai, John F Valentine, Mary P Bronner, Teresa A Brentnall, Sheng Pan, Ru Chen

Faculty, Staff and Student Publications

Metabolic plasticity allows cancer cells to survive under adverse conditions. To investigate the role of mitochondrial chaperone tumor necrosis factor receptor-associated protein 1 (TRAP1) in this process, we used CRISPR/Cas9 mediated genetic deletion to knock out (KO) TRAP1 in colon cancer cells. Depletion of TRAP1 triggered a series of events: induced metabolic reprogramming, increased glycolytic flux, downregulation of mitochondrial complex I, and elevated ROS generation. TRAP1-deficient cells showed tolerance to Oxidative Phosphorylation (OXPHOS) inhibitors and exhibited a higher extracellular acidification rate (ECAR). Additionally, TRAP1 depletion activated hypoxia response elements (HREs) and upregulated HIF1A target genes such as GLUT1 and MCT1. …

Risk Score Roulette: A Cautionary Tale Of Polygenic Risk Score Reliability, Diane Xue, Elizabeth E Blue, Alexis C Wood, Jerome I Rotter, Alison E Fohner

Children’s Nutrition Research Center Staff Publications

Genetic risk prediction for Alzheimer's disease (AD) has high potential impact, yet few studies have assessed the reliability of various polygenic risk score (PRS) methods at the individual level. Here, we evaluated the reliability of AD PRS estimates among 6338 participants from the Multi-Ethnic Study of Atherosclerosis. We compared four PRS models that have been previously associated with dementia risk. Despite similar population-level performance metrics, inter-model reliability of individual-level risk assessment was low, even among individuals classified in the top and bottom deciles. These findings raise serious concerns about the downstream application of PRS for guiding interventions for AD.

Global Cooling Watch 2025, Omar Abdelaziz, Ray Gluckman, Ian Hamilton, Radhika Khosla, Lily Riahi

Research Collection College of Integrative Studies

The second edition of UNEP’s Global Cooling Watch Report takes a deep dive into one of the decade’s most urgent challenges: surging heat, soaring cooling demand, and stark inequalities in access. Produced by the UNEP-led Cool Coalition, the report provides a comprehensive assessment of the rapidly growing global demand for cooling and the need for climate-friendly solutions to the issue. The 2025 report builds on the first Global Cooling Watch (2023), which established the Sustainable Cooling Pathway framework. The new edition deepens analysis of intensifying extreme heat and its effect on cooling demand, improved assessment of passive cooling, and coverage …

Home And Religion, Emma Alexandra Grimley, Orlando Woods, Lily Kong

Research Collection College of Integrative Studies

This chapter explores how religious practices and identities intersect with the home as both a construct and physical locale and surveys the social science literature on religion in/and the home. We argue that the shifting space-times of religious practice reflect and influence the changeable and porous boundaries between public and private space, the home and beyond the home. This influence is shown by reference to three areas of focus: the home as a private space of and for religion; the use of religion to cross social and spatial boundaries of home; and the reconfiguration of the home in religious practice …

Poster Abstract: Field Evaluation Of Body Thermoregulation-Based Dynamic Bedroom Air Temperature Control To Improve Sleep, Wenhao Zhang, Stefano Schiavon, Clayton Miller

Research Collection College of Integrative Studies

Sleep is essential for human health and well-being. Previous lab-based studies have shown that dynamically adjusting bedroom temperature according to body thermoregulation can improve sleep quality and thermal satisfaction. However, such strategies have not been tested in real-world settings, and there is a lack of practical solutions developed for residential buildings. In this work, we test a body thermoregulation-based dynamic air conditioning (AC) control strategy designed for typical Singapore homes and evaluated it in a pilot field deployment. The preliminary findings suggest that the proposed strategy can be successfully implemented in real bedroom settings and may improve sleep outcomes.

Beyond Green Claims: How Different Content And Visual Media Shape Sustainability Communication On Social Media Platforms, Elnur Nabivi

European Management Studies

Purpose: This study examines the types of green marketing content that drive consumer engagement on social media, focusing on content categories, visual media effectiveness, and platform-specific strategies.

Design/methodology/approach: Employing a mixed-method content analysis, the research analysed 3,138 posts from six FMCG companies’ corporate accounts on Facebook and X (formerly Twitter) over one year. Posts were coded for green marketing relevance, content type, visual media, and engagement metrics (likes, comments/replies, shares/reposts).

Findings: Green marketing content, comprising 38.4% of posts, outperformed non-green content in engagement across all metrics. Content highlighting environmentally responsible practices and green product promotion drove the …

Remaking The Smart City Through The Covid-19 Pandemic: Seoul, Singapore, Taipei, Orlando Woods, I-Chun Catherine Chang, Haeran Shin

Research Collection College of Integrative Studies

The COVID-19 pandemic has proven to be a catalysing force for urban innovation in general, and for smart city agendas specifically, throughout the world. From the development of contact tracing technologies and the enforcement of quarantine and safe distancing measures, to the dissemination of public health information and advancement of telehealth rollouts, the promises of “smart” technologies to reveal and govern patterns of socio-spatial contact and mobility can be found at the core of effective city management during the pandemic. Arguably nowhere is this truer than in the cities of Seoul, Singapore and Taipei, where the Asian developmental state was …

Global Standards And Local Ambitions Across Green Taxonomies: Climate Change Mitigation From The European Union To South Africa, Theodor Florian Cojoianu, Andreas G. F. Hoepner, Ifigeneia Paliampelou, Anh Vu, Dariusz Wojcik

Research Collection College of Integrative Studies

Country-level green finance taxonomy standards have emerged to provide clarity on environmentally-sustainable economic activities to attract investment, protect financial services consumers, and counteract greenwashing. This paper adopts the Global Production and Financial Network (GPFN) approach and analyses factors affecting the climate change mitigation ambition level of the South African Green Finance Taxonomy (RSA GFT) in comparison with the EU taxonomy, which served as a model for South Africa's (RSA) regulators. It accounts for (i) the interplay between EU’s and RSA’s production and financial networks, and (ii) RSA’s willingness to attract European funding for sustainable development. We find that EU private …

Enhancing Urban Energy Modeling: A Case Study Of Data Acquisition, Enrichment, And Evaluation In Berlin, Felix Rehmann, Martín Mosteiro-Romero, Clayton Miller, Rita Streblow

Research Collection College of Integrative Studies

Urban Building Energy Modeling (UBEM) has become a critical tool for developing local heating and cooling plans, as required by the European Union. Despite growing interest, the reproducibility and reliability of UBEM studies remain limited due to data scarcity and workflow complexity. This paper presents a comprehensive framework to evaluate the data pipeline in UBEM, with a particular focus on data acquisition, enrichment, simulation, calibration, and information application. The approach applies three distinct UBEM workflows (CityEnergyAnalyst, DistrictGenerator, and SimStadt) to the Mierendorffinsel district in Berlin, Germany. We compare the based on quantitative performance metrics and qualitative framework criteria. The results …

A Dataset Exploring Urban Comfort Through Novel Wearables And Environmental Surveys, Patrick Chwalek, Sailin Zhong, Nathan Perry, Tianqi Liu, Clayton Miller, Seiied Hamed Alavi, Denis Lalanne, A. Joseph Paradiso

Research Collection College of Integrative Studies

This study presents a comprehensive dataset capturing indoor environmental parameters, physiological responses, and subjective perceptions across three global cities. Utilizing wearable sensors, including smart eyeglasses, and a modified Cozie app, environmental and physiological data were collected, along with pre-screening, onboarding, and recurring surveys. Peripheral cues facilitated participant engagement with micro-EMA surveys, minimizing disruption over a 5-day collection period. The dataset offers insights into urban comfort dynamics, highlighting the interplay between environmental conditions, physiological responses, and subjective perceptions. Researchers can utilize this dataset to deepen their understanding of indoor environmental quality and inform the design of healthier built environments. Access to …

Assessing The Adoption And Implementation Of Port Community System (Pcs) In Thailand, Boodsayapan Thitanon, Chalongrach Waraporn

World Maritime University Dissertations

No abstract provided.

Determinants Of Containership Time Charter Rates : A Hedonic Pricing Approach, Khusnul Khotimah, Thi Thu Ha Doan

World Maritime University Dissertations

No abstract provided.

Estimating The Value Of 5c’S Of Credit Analysis In Newbuilding Ship Loans, Gwanglyong Park, Xinyi Ruan

World Maritime University Dissertations

No abstract provided.

Legal Analysis On The Use Of Maritime Drones In Law Enforcement Operations, Da Ye Kim

World Maritime University Dissertations

No abstract provided.

Assessing The Effectiveness Of Extension Services On Aquaculture Development In Ashanti Region, Ghana, Bismark Essel

World Maritime University Dissertations

No abstract provided.

Assessing Marpol Annex V Port Reception Facilities Of The National Port Of Liberia (Freeport Of Monrovia), Barbara Wade Cummings

World Maritime University Dissertations

No abstract provided.

Evaluating The Implementation Of The Ballast Water Management Convention In Nigeria : The Possible Contribution Of The Experimentalist Governance Approach, Muhammad Ahmad

World Maritime University Dissertations

No abstract provided.

The Role Of Marine Spatial Planning (Msp) In Managing Conflicts Between Marine Resource Users In Tanzania, Ramadhani Juma Shausi

World Maritime University Dissertations

No abstract provided.

Governance In Nigeria’S Extended Continental Shelf : Implications For Domestic Regulation, Mark Terver Mailumo

World Maritime University Dissertations

No abstract provided.