Digital Commons Network^™

Dielectrophoretic Biomarkers For Cancer Detection And Cell Analysis: Integrating Microfluidics And Ai, Raphael Olumide Oladokun

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pancreatic ductal adenocarcinoma (PDAC) carries a five-year survival rate of 13%, driven largely by late-stage diagnosis and the absence of sensitive noninvasive screening tools. Current diagnostic methods lack the sensitivity to detect disease-associated systemic changes at early and precancerous stages, representing a critical clinical gap. This dissertation addresses the central hypothesis that PDAC and its pathological subtypes induce measurable, disease-associated electrophysiological changes in circulating PBMCs that can serve as correlative liquid-biopsy biomarkers. To test this hypothesis, this work asks: can dielectrophoresis (DEP), impedance and zeta potential analysis with AI-assisted classification distinguish benign pancreatic conditions (non-malignant control), precancerous lesions (primarily IPMNs), …

The Coal Mining Kids: Constructing A Working-Class Gay World In West Virginia (1900-2001), Hannah Ford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Located in southwestern West Virginia, Bluefield has been the site of Bluefield State University, the Norfolk & Western Railway headquarters, the Baldwin-Felts Detective Agency, and the Shamrock Bar. The Shamrock was a gay bar opened by Helen Compton, in operation from the late 1960’s until 2001. Bluefield’s early industrial success in coal mining and the railroad quickly urbanized the space between 1900 and 1930, facilitating a diverse, working-class cultural foundation for Bluefield’s gay world. In the 1960’s, urban renewal efforts initiated by the War on Poverty, in combination with public awareness of homosexuality induced by the Lavender Scare, set the …

Multi-Sensor Fusion For Manual Wheelchair State Estimation, Kathylee Pinnock Branford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Kathylee Pinnock Branford Manual wheelchairs are essential to mobility and independence, yet accurately characterizing wheelchair motion in real-world environments remains challenging. Inertial measurement units (IMUs) provide a practical means of estimating wheelchair kinematics, but existing trajectory-reconstruction studies have largely been limited to short, controlled trials. This report developed and evaluated a four-IMU framework comprising one sensor on each rear wheel and two sensors on the wheelchair frame. Three orientation algorithms (VQF, Mahony AHRS, and Madgwick AHRS), two accelerometer ranges (±2 g and ± 4 g), and calibrated and uncalibrated sensor data were evaluated during indoor motion-capture trials and a outdoor …

Energy Partition And Heating Mechanisms In Asymmetric Antiparallel Magnetic Reconnection, Aleksandra Abova-Volkova

Graduate Theses, Dissertations, and Problem Reports (ETD)

Magnetic reconnection is a fundamental plasma physics process whereby a change in magnetic field connectivity drives the conversion of magnetic energy into energy of the surrounding plasma, often explosively. One of the many settings where it occurs is at the dayside magnetopause of Earth and other planets, where the magnetic field, number density, and/or temperature in the plasmas on the two sides upstream of the reconnection site are asymmetric. How the incoming magnetic energy gets partitioned into outgoing energies during asymmetric reconnection remains an outstanding question; of special interest is the fraction that gets converted into internal energy of ions …

Assessing Mobility And Upper Extremity Function Beyond Laboratory Settings In Manual Wheelchair Users With Spinal Cord Injury Using Inertial Measurement Units, Kathylee Pinnock Branford

Graduate Theses, Dissertations, and Problem Reports (ETD)

Manual wheelchair users with spinal cord injury (SCI) face chronic, repetitive shoulder demands that far exceed those of able-bodied daily life, placing them at high risk for rotator cuff pathology and long-term upper extremity dysfunction. Despite this burden, existing research has relied predominantly on brief laboratory assessments that capture only a narrow part of real-world mechanical demands, leaving critical gaps in the identification of true injury risk factors. This dissertation developed and applied inertial measurement unit (IMU)-based methods and novel algorithms to characterize arm use, mobility, and environmental context during a full week of free living behavior in 25 manual …

Process Optimization And Quality Control In Manufacturing Of Frp Composites By Vartm, Mohamed Emadeldin Omar Abdelhadi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Railroad tank cars, being one of the most used means of transportation of hazardous material across the country, have not been structurally upgraded for a long time. Thus, the Pipeline and Hazardous Materials Safety Administration (PHMSA), a United States Department of Transportation (USDOT) agency, released a rule for safer transportation of highly hazardous and flammable liquids by railroad to enhance the design specifications of both the new and existing tank cars in May 2015. A multifunctional composite jacket is proposed as a new material to structurally enhance the railroad tank cars to withstand any accidents that could happen en route. …

"If I Knew Then What I Know Now": Analyzing Online Narratives Of Regretful Parents, Marina Binti Mohd Hamdan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This exploratory study seeks to understand the extent to which parenthood affects individual self-actualization by identifying the trade-offs, compromises, and sacrifices that parents make within the context of family life. Using a thematic analysis of 221 archived posts from the Reddit community r/regretfulparents, this study examines how social pressures surrounding parenthood may be related to experiences of parental regret. Specifically, it identifies two domains: pressures to be parents, including social norms, familial coercion, and interpersonal conflict, and pressures of parenting, including issues of labor, number of children, and challenging children. Across these domains, this study explores how tensions between personal …

Nuclear Aurka-Driven Early Dissemination Of Breast Cancer Uncovers Opposing Roles Of Hif-1a And Hif-1b In Metastasis, Abha Maskey

Graduate Theses, Dissertations, and Problem Reports (ETD)

Metastatic progression is the primary cause of mortality in the majority of solid tumors, including breast cancer (BC). The triple-negative breast cancer (TNBC) is the most aggressive BC subtype with the highest recurrence rates within 1-3 years post-diagnosis, and worst overall survival outcomes. Early distant recurrence of cancer is enabled by early disseminated tumor cells (eDTCs), yet the mechanisms of early dissemination remain poorly defined. Hypoxia-inducible factor 1 (HIF1) is a transcription factor normally induced under hypoxia to enable cell survival. It is the major cause of metastasis from hypoxic tumors. In our work, we demonstrated that the nuclear Aurora …

Intersectionality In Discrimination, Posttraumatic Stress Symptoms, And Suicide Ideation: Black Americans In Appalachian Contexts, Jailyn May Wilson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Racial discrimination is recognized as a race-based traumatic stressor that has been associated with posttraumatic stress symptoms (PTSS) and suicide ideation (SI). However, there remains a paucity of research investigating the impact of racial and other forms of discrimination on understudied populations, including Black Americans residing in Appalachian regions. The current study used an online self-report survey to examine PTSS as a mediator in the association between perceived discrimination and SI and to examine the moderating effect of the number of identity factors with which one experienced discrimination (i.e., race, gender, region) on the relationship between perceived discrimination and PTSS. …

Wellness Policies In Action: A Wscc-Based Comparative Analysis Of District-Level Policy And School-Level Practices, Leanne Denise Watson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The United States Department of Agriculture utilized the policy platform to implement and reinforce federal mandates aimed to improve health outcomes for student-aged youth, requiring all schools participating in the National School Lunch and/or Breakfast Program to develop a written wellness policy beginning with the 2016-2017 school year. However, the resulting impacts on school environments are unclear. The Whole School, Whole Community, Whole Child (WSCC) model is a recommended framework to influence wellness policies and school practices and support the holistic development of students. Nonetheless, current literature calls for more research applying the model to support its use. The purpose …

Cross-Cue Reactivity Of Nicotine And Cannabis Vaping Products, Madeline Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

SIGNIFICANCE. The rewarding properties of nicotine increase when administration is paired with external stimuli, or “cues”, including those related to vaping products. Given the visual similarity between nicotine vaping products (NVPs) and THC vaping products (TVPs), exposure to one product may elicit reactivity (e.g., craving, urge to use) to the other. The present study aimed to develop a set of cues capable of differentiating NVPs from TVPs to inform investigations of cross-cue reactivity. METHODS. Images (n=61 NVPs; n=64 TVPs; 7=unspecified) were obtained via an online search using both generic (“vape”, “vape pen”) and product-specific (e.g., “nicotine vape”, “dab pen”) keywords …

Exploring Youth Soccer Coach Recruitment Practices, Han-Earl Lim

Graduate Theses, Dissertations, and Problem Reports (ETD)

This qualitative single-case study examined how a private-interest, nonprofit youth soccer club in the Midwestern United States recruits coaches, what coach qualities are prioritized in hiring, and how organizational and sociocultural conditions shape those practices. The study was guided by three research questions focused on (a) recruitment and early support processes, (b) hiring criteria and decision rationales, and (c) the organizational and sociocultural contexts influencing recruitment. Data were generated through five semi-structured interviews with four participants who were involved in recruitment practices (the Executive Director, the Assistant Director, and two full-time head coaches). Additional sources included an organizational mapping survey …

Comparison Of Push-Pull Air Curtain To Overhead Downdraft Systems On Aerosol Containment And Distribution In Controlled Environments, Manal Mutairi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Comparison of Push-Pull Air Curtain to Overhead Downdraft Systems on Aerosol Containment and Distribution in Controlled Environments.

This dissertation looks at the ongoing issue of tiny airborne particles created during medical and dental procedures and evaluates ways to reduce the risk of exposure for healthcare workers and patients to these particles. The first study focuses on the size, how long particles stay in the air, and how far they travel from common medical procedures that create aerosols, showing that tiny particles (less than 5 µm) can stay in the air long enough to escape basic droplet controls. As a …

Exploring The Application Of Wastewater Monitoring To Determine The Prevalence And Diversity Of Influenza On The Campus Of West Virginia University, Lakyn Renee Sanders

Graduate Theses, Dissertations, and Problem Reports (ETD)

Influenza viruses (IV) affect populations globally, with the World Health Organization (WHO) estimating that IV are responsible for an estimated 3-5 million infections and 290,000 – 650,000 deaths annually^1–3. More specifically, the 2019-2020 IV season is associated with an estimated 38 million illnesses and 22,000 deaths in the United States alone⁴. IV are rapidly spread between individuals through direct contact with infected individuals, contaminated surfaces, or aerosolized virus particles². Traditional IV infection monitoring relies on clinical screening of patients presenting influenza-like symptoms, commonly through the use of multi-target panel tests such as the BioFire …

Do The Adaptogenic Mushrooms, Cordyceps Militaris And Hericium Erinaceus, Produce Caffeine-Like Discriminative-Stimulus Effects?, Stephen James Cullinan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Caffeine is one of the most widely consumed drugs in the United States, but its excessive consumption increases the risk of heart disease, sleep problems, and anxiety. In response to these issues, there has been a rise in efforts to find safer alternatives to caffeine while maintaining similar subjective effects. The adaptogenic mushrooms, cordyceps and lion’s mane, are claimed to have similar energy- and mood-enhancing effects as caffeine; however, there is limited research directly comparing their effects to caffeine. A drug-discrimination paradigm, in which rats were trained to discriminate between caffeine and saline, was used to compare the discriminative-stimulus effects …

Women In Music: Experiences Of Women Oboists In Us Symphony Orchestras, Brianna Corgel

Graduate Theses, Dissertations, and Problem Reports (ETD)

The evolution of women in American music has been a gradual and ongoing process. Historically, women were largely excluded from public musical life and did not achieve widespread acceptance as professional musicians until the 20^th century, largely due to prevailing cultural and societal expectations. In the 19^th century, women typically performed in domestic settings for small gatherings of family and friends. Female instrumentalists were generally limited to the piano and a few string instruments, while wind instruments—such as the oboe—were discouraged because they were considered unladylike or visually unappealing when played. The 20^th century saw the emergence …

Improving The Continuity Of Care For Opioid Use Disorder: Investigating Fentanyl Overdose Outcomes And Pharmacy-Level Buprenorphine Access, Lyn Yuen Choo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Opioid overdose (OD) deaths remain a leading cause of injury-related death in the United States (US) and approximately 76,516 drug OD deaths were predicted in the past year prior to April 2025. While previous research has focused on fatal opioid ODs, less is known about outcomes following non-fatal opioid ODs (NFOO) involving fentanyl. Patients who experienced a fentanyl OD may develop significant morbidity and inpatient mortality due to fentanyl’s high potency, rapid onset of action, and decreased response to naloxone. Research efforts to improve continuity of care for opioid use disorder (OUD) are highly needed to ensure appropriate follow-up care …

A Guide To The Style Of Domenico Scarlatti With An Analysis Of Six Sonatas, Joseph Mcdermott

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document aims to inform pianists preparing to perform keyboard sonatas by Domenico Scarlatti (1685-1757). Readers will learn about Scarlatti's professional life and his compositional characteristics. Analyses of six sonatas, the influence of Spanish traditional music, and details on performance practices are used to illustrate his style. The six sonatas included in this study are Sonata in G Major, K. 63; Sonata in C minor, K. 22; Sonata in F minor, K. 238; Sonata in F minor, K. 239; Sonata in D Major, K. 490; and Sonata in D Major, K. 491. The influence of Spanish traditional music is discussed …

Dispositional Mindfulness And Autonomic Reactivity, Thomas Dylan Boyer

Graduate Theses, Dissertations, and Problem Reports (ETD)

To explain how different facets of mindfulness were associated with task performance in distinctive ways, Lindsay and Creswell (2017) proposed the Monitor and Acceptance Theory (MAT). According to this model, the mindfulness facet of monitoring present state awareness was thought to influence task performance differently than the nonjudgmental acceptance facet of mindfulness, but no studies to date have examined this model with respect to an individual's physiological response to stressful tasks. Because dispositional mindfulness has been associated with both increased and decreased physiological and affective reactions to stress in previous studies, the present study tested whether the Monitoring facet of …

Timing, Spectral, And Single-Pulse Properties Of Radio Pulsars, Evan Francis Lewis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Radio pulsars are highly magnetized, rapidly rotating neutron stars which emit beams of radio waves from their magnetic poles. Their incredible rotational stability allows for their use in a variety of high-energy astrophysical studies, though the details of their internal structure and emission mechanisms are still unknown. We use observations of a cross-section of the pulsar population in order to investigate the nature of their evolution, their emission properties, and their environments.

First we derive timing solutions for three pulsars discovered through their sporadic single pulses. It was possible to obtain timing solutions through a set of single-pulse times of …

Gcn5l1-Mediated Lysine Acetylation Regulates Mitochondrial Dysfunction In The Aged Heart, Jackson Edmond Stewart

Graduate Theses, Dissertations, and Problem Reports (ETD)

For over a century, cardiovascular disease (CVD) has been and remains the leading cause of death globally. CVD risk and severity increase significantly with advanced age, and mitochondrial dysfunction has been implicated in the pathogenesis of aging. Furthermore, mitochondrial health is crucial for cardiac function, as one-third of total cardiomyocyte volume is occupied by mitochondria in order to meet the high energy demand of contractile and relaxational function. It is thus crucial that we understand the pathological mechanisms driving mitochondrial dysfunction in aging and how this contributes to age-associated cardiovascular disease. The current manuscript aims to fill this knowledge gap …

Three Essays On Urban Transportation, Daniel Amorim Souza Centuriao

Graduate Theses, Dissertations, and Problem Reports (ETD)

Urban environments are shaped by dynamic interactions between infrastructure development, policy interventions, and episodic demand shocks, all of which can generate unintended externalities in the form of motor vehicle collisions, injuries, and fatalities. This dissertation investigates the determinants of traffic safety in dense urban settings, with a particular focus on how different sources of urban activity affect collision risk and severity. Using New York City as a case study and leveraging high-resolution spatial and temporal data, I examine three distinct but interconnected mechanisms: construction activity, traffic safety policies, and large-scale events.

The first chapter analyzes the impact of urban development, …

Preliminary Analysis Of Soil Volatile Organic Compounds As Soil Health Indicators, Thorne Taul

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the past decade, soil health (SH) has gained popularity due to the novelty of sustainable soil use related to human wellbeing. Soil health describes the overall quality of soil and its capacity to function over time. As interest in SH grows, researchers continue to explore new soil health indicators that can improve knowledge on how soil health is assessed and managed. Some indicators directly reflect soil health status, and some indirectly relate to processes and functions occurring within the soil. Volatile organic compounds (VOCs) are naturally occurring compounds that play a role in multiple soil processes. They are associated …

The Effects Of Gesture And Age On Jurors’ Perceptions Of Child Witnesses, Jihan Hani Rashed

Graduate Theses, Dissertations, and Problem Reports (ETD)

Jurors’ perceptions of child witnesses can have significant influence in judicial outcomes, particularly in cases where children’s testimony is central (Goodman et al., 1997; Goodman et al., 1998; Castelli et al., 2005). The role of gesture in shaping jurors’ perceptions is underexplored. Studies highlight that gesture enhances memory recall and reduces cognitive load, making it an important component of effective communication (Goldin-Meadow et al., 2001; Hudson & Sheffield, 1998; Cook & Fenn, 2017). The present research consisted of two studies examining whether child witnesses’ use of gesture during recall of an alleged crime affected jurors’ perceptions of witness credibility and …

Rurality, Infant Feeding Practices, And Perinatal Cardiovascular Health Behaviors, Elly Marie Marshall

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Cardiovascular diseases are a leading cause of pregnancy-related deaths in the United States, with higher rates in rural areas. While limited healthcare access contributes to this disparity, other factors remain unclear. Breastfeeding provides long-term protection against cardiovascular disease, but modern infant feeding practices are complex and may impact maternal cardiovascular health (CVH) in unexpected ways. Healthy CVH behaviors of sufficient sleep duration, adequate physical activity, a healthy diet, and nicotine avoidance can enhance protection against future cardiovascular disease risk. Therefore, the aims of this dissertation are to 1) assess associations between rurality and first trimester CVH behaviors; 2) synthesize …

Manipulating Unit Price During Alternative Reinforcement Schedule Thinning In A Concurrent- Schedules Arrangement, Katherine A. Cucinotta

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reinforcement schedule thinning, a critical component when addressing target behavior using differential reinforcement of alternative behavior schedules, aims to decrease alternative reinforcement rates while maintaining suppression of a target response (Davis et al., 2023; Hagopian et al., 2011). When multiple response options are available, manipulating the relative unit price across options alters the relative efficiency with which reinforcement is obtained and can shift responding toward an alternative behavior. Thus, altering the relative unit price can be a beneficial tactic when reinforcement for a target behavior cannot be withheld. Using concurrent-schedule arrangements, two experiments evaluated the conditions under which target response …

Assessing Water Quality In Drinking Water Distribution Systems: Rural Infrastructure Challenges, Hydrodynamic Effects On Biofilms, And Biofilm Contribution To Disinfection By-Products Formation, Vinila Vasam

Graduate Theses, Dissertations, and Problem Reports (ETD)

Access to clean and safe drinking water remains one of the most persistent public health and environmental challenges in the United States. Maintaining water quality after leaving the treatment plant in drinking water distribution systems (DWDS) is a complicated and often overlooked challenge. Most research focuses on water treatment and source water quality, but not on the miles of pipe that follow. Aging infrastructure, complex flow patterns in the DWDS, and limited resources make water quality management especially difficult. This dissertation includes three core studies: 1) A system-level analysis of small and rural utilities in the geographically complex region of …

Pbmc Transcriptome Analysis Following Lps Stimulation Reveals Distinct Inflammatory Strategies In Feed-Efficient And Inefficient Beef Cattle, Yarahy Leal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Improving feed efficiency is a primary objective in livestock production, serving as a major driver of profitability and sustainability in beef cattle operations as feed costs continue to rise. Residual feed intake (RFI) and residual average daily gain (RADG) are widely recognized indicators of feed efficiency and are commonly used as selection traits to reduce production costs and environmental impact. Previous research suggests a functional shift in transcriptional activity associated with immunity depending on efficiency phenotype, with evidence of greater immune system robustness in more efficient animals. This dissertation examines the molecular mechanisms linking feed efficiency and immune competence and …

Bringing Patriarchy To Looksmaxxing: Identification And Exploration Of Strategies To Restabilize The Patriarchy In The Looksmaxxing Community, Mackenzie Grace Freeman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research literature on looksmaxxing is subsumed under the incelosphere, which is acknowledged as a white and male supremacist space. Yet, researchers fail to attend critically to the structure of patriarchy, choosing instead to base their analyses within the mainstream hegemonic masculinity paradigm. Using a Bourdieusian framework, this project fills a gap in the literature by transcending the mainstream paradigm to explain how the looksmaxxing community uses the body as a site of crisis management in response to patriarchal backlash, with the ultimate goal of restabilizing the patriarchy. This project uses a community-led survey from a popular online looksmaxxing community. This …

The Bleeding Edge Of Misogyny: How Gender-Based Harassment And Infrastructural Neglect Shape Menstrual Distress, Mackenzie J. Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite growing attention to menstrual health, organizational research has largely overlooked how workplace conditions shape menstrual experiences. Dominant perspectives frame menstruation as an individual issue that negatively affects work, neglecting how features of the work environment – such as gender-based harassment and access to menstrual-related infrastructure – contribute to menstrual distress. Addressing this gap, this dissertation adopts a bidirectional perspective, examining how social and material workplace conditions shape menstrual distress, defined as the extent to which menstrual symptoms interfere with daily functioning. Drawing on the transactional model of stress and objectification theory, I hypothesized that gender-based harassment would predict menstrual …