Chemical Engineering Commons^™

Acoustothermal Transfection For Cell Therapy, Xiufang Liu, Ning Rong, Zhenhua Tian, Joseph Rich, Lili Niu, Pengqi Li, Laixin Huang, Yandai Dong, Wei Zhou, Pengfei Zhang, Yizhao Chen, Congzhi Wang, Long Meng, Tony Jun Huang, Hairong Zheng

Faculty Publications

Transfected stem cells and T cells are promising in personalized cell therapy and immunotherapy against various diseases. However, existing transfection techniques face a fundamental trade-off between transfection efficiency and cell viability; achieving both simultaneously remains a substantial challenge. This study presents an acoustothermal transfection method that leverages acoustic and thermal effects on cells to enhance the permeability of both the cell membrane and nuclear envelope to achieve safe, efficient, and high-throughput transfection of primary T cells and stem cells. With this method, two types of plasmids were simultaneously delivered into the nuclei of mesenchymal stem cells (MSCs) with efficiencies of …

Production Of Mrna Vaccine For Influenza, Christina Miller

Honors Theses

The objective of this project is to design a process to produce an mRNA influenza vaccine that serves as an alternative to conventional influenza vaccines, replacing current options with innovative mRNA technologies. Given that older populations are most likely to follow up on immunizations consistently, and the increasing average age worldwide, the market for this project appears to be expanding in the next 80 years. Additionally, experts predict more frequent mutations and outbreaks of viral disease from a variety of factors including globalization and global temperature trends. Rapid response to these viral outbreaks will continue to be as, or even …

Electrodeposition Of Gadolinium Metal Thin Films For Neutron Phase Contrast Imaging, Mary Louise Gucik

Chemical and Biological Engineering ETDs

A rare earth metal, gadolinium has unique magnetic properties and the largest neutron absorption cross section making it highly sought after in emerging technology. However, unlike most metals, it is difficult to work with and lacks a damascene process. When incorporated into alloys, gadolinium’s properties are muted and the film thickness must increase to account for the low concentration. Gadolinium metal films have been deposited successfully through sputter coating, but these films have density deficiencies and are limited to line-of-sight substrates. In this work, the deposition of gadolinium metal thin films was investigated for use on complex geometries via electrophoretic …

Improved Modeling And Application Of Data Assimilation In Gas Influx Management During Managed Pressure Drilling, Chen Wei

LSU Doctoral Dissertations

The management of gas influxes in a drilling well has been considered one of the most critical topics for maintaining petroleum drilling safety and efficiency. Reliable modeling of gas influx behaviors in a wellbore or a marine riser greatly benefits the improved understanding of a gas kick event and the development of optimized influx management strategies. However, the current studies on transient multiphase flow modeling show varying limitations on both the model rigorousness and the overall numerical efficiency. Meanwhile, the existence of great system uncertainties, such as the unclear influx sizes and distributions in the well/riser, has brought significant challenges …

Seeding Effects Of Submicron Caal-No3 Ldh Particles On The Hydration And Properties Of Portland Cement And Sulfoaluminate Cement Pastes, Sukanta K. Mondal, Monday Uchenna Okoronkwo

Chemical and Biochemical Engineering Faculty Research & Creative Works

Layered Double Hydroxide (LDH) Is Reported To Improve The Durability Of Concretes, Primarily Due To Its Ability To Exchange Anionic Species, Including Chloride, Which Is Implicated In Corrosion-Driven Durability Issues. However, There Is No Comprehensive Study Investigating The Effect Of LDH On The Properties Of Different Cement Systems At Both Early And Mature Ages. In This Study, The Early Age And Mature Age Properties Of Portland Cement (OPC) And Calcium Sulfoaluminate (CSA) Cement Pastes Seeded With Submicron-Sized Calcium Aluminum-NO3 LDH (CaAl-NO3 LDH) Were Investigated. The Effects Of The 1–5%mass Dosage Of LDH On The Hydration Of Both Cement Systems Were …

Seeding Effects Of Submicron Caal-No3 Ldh Particles On The Hydration And Properties Of Portland Cement And Sulfoaluminate Cement Pastes, Sukanta K. Mondal, Monday Uchenna Okoronkwo

Chemical and Biochemical Engineering Faculty Research & Creative Works

Layered double hydroxide (LDH) is reported to improve the durability of concretes, primarily due to its ability to exchange anionic species, including chloride, which is implicated in corrosion-driven durability issues. However, there is no comprehensive study investigating the effect of LDH on the properties of different cement systems at both early and mature ages. in this study, the early age and mature age properties of Portland cement (OPC) and calcium sulfoaluminate (CSA) cement pastes seeded with submicron-sized calcium aluminum-NO3 LDH (CaAl-NO3 LDH) were investigated. the effects of the 1–5%mass dosage of LDH on the hydration of both cement systems were …

Assessing Hydrolysis Performance Of Ce(Oh)4@Pim-1 Composites Functionalized With Amidoxime, Aldoxime, And Carboxylate Groups Toward Dimethyl 4-Nitrophenylphosphonate, A Nerve Agent Simulant, Peter O. Aina, Sukanta K. Mondal, Ali A. Rownaghi, Fateme Rezaei

Chemical and Biochemical Engineering Faculty Research & Creative Works

In recent years, significant attention has been directed toward evaluating the reactivity of diverse polymers to gauge their catalytic activity against hazardous chemicals. Among these polymers, pim-1 has emerged as an exceptional candidate due to its noteworthy attributes including high surface area, excellent solubility, and the capability to fine-tune the nitrile functional groups along its polymeric backbone. In this study, we explored the impact of different functional groups, such as amidoxime (ax), aldoxime (ox), and carboxylate (cooh), on the degradation of dimethyl 4-nitrophenylphosphonate (dmnp) in comparison to pure pim-1 and their respective ce(oh)4-loaded polymer composites. Remarkably, the pim-1-ax analog exhibited …

Research On 3d Printing Resin Exposure Properties And Its Application On Centrifugal Microfluidic Platform Based On Fluorescence Detection, Zheng Qiao

LSU Doctoral Dissertations

This dissertation encapsulates significant advancements in the field of SLA 3D printing and centrifugal microfluidics. Central to the research is the development of a novel mathematical model for predicting trapped resin thickness in SLA 3D printing, a groundbreaking contribution that addresses a critical aspect of printing intricate structures. This model, the first to establish a mathematical relationship for resin thickness, is rooted in a comprehensive study of the resin curing process. The research leverages the concept of 'critical dosage' for resin curing, leading to a more refined and theoretically grounded approach for calculating curing thickness. Experimentation further validates the model, …

The Application Of Poly-Ε-Caprolactone Nanoparticles For Endotoxin Removal, Mikaela Ritchie

Undergraduate Research Conference at Missouri S&T

Lipopolysaccharides (LPS), also known as endotoxins, are toxins released from the cell walls of gram-negative bacteria upon their death. Ingestion of LPS by humans or animals can initiate inflammatory immune responses, resulting in blood vessels dilation, organ dysfunction and potential death. These toxins contaminate a variety of manufacturing processes from the pharmaceutical industry to animal feed production, posing a risk to humans and animals alike. This study details the production of PCL nanoparticle capable of removing LPS from water and a preliminary analysis of the removal efficiency. The outcomes of this research hold significant implications for industries reliant on bacterial …

Lung Cancer Drug Study Using Alc Nanoparticles, Noah Sparks

Undergraduate Research Conference at Missouri S&T

In a previous project it was determined ALC polarized lipid nanoparticles have a high transfection efficiency in some lung cancer cell lines. Building off this information, these ALC particles will now be tested with an actual lung cancer drug. This process will consist of searching articles for promising lung cancer drugs and selecting one for use. The ALC particles will then undergo a comprehensive drug study using multiple lung cancer cell lines with the selected drug. Aspects such as drug release rate, transfection rate, cytotoxicity, etc. will be determined. The goal is to determine whether ALC nanoparticles show real promise …

Biosensors For Antibacterial Medical Applications, Ryan Fagan, Shelby Wallen

Undergraduate Research Conference at Missouri S&T

Healthcare associated infections are significant concern in medicine and as antibiotics are becoming less effective, we need new alternative methods to treat them. We are testing various materials that can be used to prevent infections in healthcare settings and in wound treatments. These materials include bioactive glass and modified hydrogel materials. From these studies we've learned these are effective in killing bacteria and preventing infections. The goal of this project is to determine the mechanism at which these materials kill bacteria. Our approach is to construct biosensor strains that can indicate bacterial stress response to antimicrobial agents. We have used …

Ionizable Lipid Nanoparticles Fabrication For Gene Delivery, Noah Sparks, Lei Xu, Hu Yang

Undergraduate Research Conference at Missouri S&T

In this project ionizable lipids were analyzed for their efficacy in delivery of mRNA in multiple metastatic lung cancer cells. Different ionizable lipids with their molar ratio in making the LNPs were analyzed in order to enhance the nucleic acid delivery to cytoplasm. Phospholipids also play a role in targeting efficacy of the LNPs to the lung cells. Different combination of ionizable lipids with multiple phospholipids were studied for physical characterization and in-vitro transcription efficiency. Transcription efficiency was then quantified using flow cytometry and optimal particles for transcription were noted.

Quantifying Asphalt Binder Uv Effects With Relaxometry, Rosalee Brown

Undergraduate Research Conference at Missouri S&T

Asphalt deterioration involves many physical and chemical changes caused by environmental factors such as automotive strain, extreme heat, and UV exposure. Asphalt binder assessment in the industry is currently based on structurally destructive testing methods and tests of physical performance without accounting for chemical structure. An emerging evaluation of asphalt binder uses relaxometry, a field of nuclear magnetic resonance (NMR) spectroscopy, to directly observe how asphalt binder is changed by causes of deterioration such as UV exposure. NMR relaxometry explores nuclear spins' interactions with their environment. This noninvasive method can provide a detailed view into the chemical environment of virgin …

Iron Doped Zeolitic Materials For Propane Dehydrogenation Catalyst, Emily Cahill, Amitava Choudhury

Undergraduate Research Conference at Missouri S&T

This research describes the synthesis of iron-doped zeolitic catalysts for the transformation of propane to propylene. The goal of this research is to develop a dehydrogenation catalyst to convert shale-gas derived cheap propane feedstock to economically valuable propylene, while at the same time making this catalyst sustainable and non-toxic to the environment. Fedoped zeolitic frameworks have shown promising activity in percentage of conversion and in selectivity. In our study we analyzed zeolites ZSM-5 and AIP0-5. These were chosen because they have differences in pore structure , channel sizes, and connectivity between channels. Most importantly the differences in the chemistry as …

Investigating The Role Of Fluid Shear Stress During Metastasis On Enhanced Breast Cancer Extravasation, Alejandra M. Ham

LSU Master's Theses

In 2022, ~288,000 American women were diagnosed with metastatic breast cancer which was the leading cause of patient mortality with ~43,000 American women succumbing to the disease. Women can be diagnosed with four different molecular subtypes of breast cancer: luminal A, luminal B, human epithelial growth receptor type 2 enriched (HER2+), and triple-negative breast cancer (TNBC). TNBC accounts for ~12% of all breast cancers, yet it has the worst outcomes. TNBC is highly invasive, with ~46% of TNBC patients developing metastatic disease. A further understanding of metastatic TNBC is needed to provide a greater understanding of how to treat this …

Thermal Frontal Polymerization – Applications In Coatings And Composites, Maria Martinez

LSU Doctoral Dissertations

Thermal frontal polymerization has long been used as a method to cure composites and coatings. However, this is the first time these applications have been used without the use of a catalyst, for the case of composites and coatings.

Chapter 2 discusses how to optimize cure-on-demand, thin layer coatings, using frontal polymerization. Previously, for the use of curing thin layer coating using thermal FP, it was known to just make the formulation viscous enough, as this would prevent premature quenching caused by heat loss. It was discovered that for a given layer thickness, a minimum viscosity and initiator concentration were …

Well Log Analysis And Interpretation For Mishrif Formation For Amarah Oil Field, Attia Attia, Ayat Ahmed Jasim, Aqeel Al-Adili, Luma Mahmoud, Mohamed Mansour Eng.

Petroleum Engineering and Gas

This study is intended to interpret of well logs for the purpose of determining petrophysical parameters for Mishrif formation in the Amarah Oil Field. This fi eld is situated in the province of Mysan ten kilometers to the southwest of Amarah and ten kilometers to the northwest of Halafaya’s fi eld, and is located southeast of the fi eld of Kamit and about 30 km. M–N cross plot as well as the Matrix Identifi cation (MID) have been applied to calculate mineralogy and lithology of the formation using well logs, cross plots, and reservoir quality index (RQI) along with fl …

Globular Protein For Surface Modification Of Cellulosecontaining Materials, Nodira Saydaliyeva

Technical science and innovation

The purpose of this work is the chemical modification of cotton fabrics with natural globular protein. Modification was carried out in the process of finishing cotton fabrics, after decoction and bleaching in a continuous mode. The effect of modification on the strength, protein content and functional groups of cotton fabric has been studied. According to the results obtained using the AUTOGRAPH AGS-N machine, it was found that the modification of cotton fabrics with a solution of globular protein increases the strength of the treated fabrics, depending on the concentration of the modifier and the temperature regime. Treatment with a highly …

Dying Of Natural Silk Deglued By Various Methods, Venera Djavdatovna Khamidova

Technical science and innovation

Products made of natural silk are indispensable for their hygienic properties and will always be in great demand. To obtain an even and intense coloration on a textile material, it is necessary to ensure its effective preparation with the most complete removal of hydrophobic contaminants (both natural and silicone oils applied during the weaving process, lubricants, etc.), which hinder the dyeing of the substrate and reduce the color characteristics of the obtained colors. Therefore, the issue of preparing natural silk for dyeing is relevant. In view of the sensitivity of the main fiber-forming component of natural silk - fibroin to …

Single Wall Carbon Nanotubes For The Detection Of Misfolded Proteins In Neuro-Degenerative Disorders, Stefanie Vining

Senior Honors Projects

Single wall carbon nanotubes (SWCNT) are one-dimensional carbon nanomaterials made up of a singular layer of graphene. These nanotubes can be functionalized with different molecules for various bio-sensing/imaging applications. SWCNTs are unique in their ability to be used as long-term multiplexed sensors due to their photostability and ability to remain fluorescent.

This project focused on the functionalization of SWCNTs to detect misfolded proteins for early detection of neuro-degenerative diseases like Alzheimers. Currently, there is no simple way to detect these misfolded proteins and diagnose for diseases like Alzheimers, Parkinsons, or ALS, and a confirmed diagnosis can take upwards of six …