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Exploring Modalities Of Productivity And Critical Quality Attributes In Fed-Batch Chinese Hamster Ovary Cell Cultures, Stephanie Klaubert 2023 Department of Chemical and Biomolecular Engineering, Clemson University

Exploring Modalities Of Productivity And Critical Quality Attributes In Fed-Batch Chinese Hamster Ovary Cell Cultures, Stephanie Klaubert

All Dissertations

Chinese hamster ovary (CHO) cells are among the most commonly used mammalian cell lines for the production of recombinant therapeutic proteins due to ease of culturing and humanlike post-translational modification capabilities. However, when compared to microbial systems, CHO cell line growth and productivity is relatively low. As the demand for monoclonal antibody (mAb) treatments continues to rise, it is critical to explore modalities for improved growth and productivity in CHO cell cultures, with the goal of increased protein concentrations and maintained product quality. Though some mechanisms for improved productivity, including temperature and cell culture media formulations, are relatively well understood; …


Iron Stress Testing Of Oilfield Produced Waters And Hydraulic Fracturing Fluids In The Bakken, Claudius Epie Njie, Kegang Ling, Md Jakaria, Olusegun Stanley Tomomewo, Adesina Fadairo, Luc Yvan Nkok 2023 University of North Dakota

Iron Stress Testing Of Oilfield Produced Waters And Hydraulic Fracturing Fluids In The Bakken, Claudius Epie Njie, Kegang Ling, Md Jakaria, Olusegun Stanley Tomomewo, Adesina Fadairo, Luc Yvan Nkok

Petroleum Engineering Student Publications

Friction reducers are a major additive in almost all water-based hydraulic fracturing fluids. Despite the important role they play in friction reduction during hydraulic fracturing, they pose a veritable threat to the permeability of tight reservoirs upon precipitation. In the industry today, the potential for such precipitation is seldom assessed and as a result, an additional contributor to the formation damage problem has been given little attention. In this work, the sensitivity and affinity of friction reducers to very low concentrations of ferric ions are exploited to provide a qualitative assessment of their propensity to precipitate in hydraulically fractured hydrocarbon …


Constructing Carbon-Encapsulated Nifev-Based Electrocatalysts By Alkoxide-Based Self-Template Method For Oxygen Evolution Reaction, En-Hui Ma, Xu-Po Liu, Tao Shen, De-Li Wang 2023 School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China

Constructing Carbon-Encapsulated Nifev-Based Electrocatalysts By Alkoxide-Based Self-Template Method For Oxygen Evolution Reaction, En-Hui Ma, Xu-Po Liu, Tao Shen, De-Li Wang

Journal of Electrochemistry

The development of green and sustainable water-splitting hydrogen production technology is beneficial to reducing the over-reliance on fossil fuels and realizing the strategic goal of "carbon neutral". As one of the half reactions for water splitting, oxygen evolution reaction has suffered the problems of sluggish four-electron transfer process and relatively slow reaction kinetics. Therefore, exploring efficient and stable catalysts for oxygen evolution reaction is of critical importance for water-splitting technology. Metal alkoxides are a series of compounds formed by the coordination function of metal ions with alcohol molecules. Metal alkoxides possess the double advantages of organic materials and inorganic materials, …


Investigation Of Foam Based Photobioreactor For The Cultivation Of Chlorella Vulgaris Cpcc 90, Anuradha Krishnan 2023 Western University

Investigation Of Foam Based Photobioreactor For The Cultivation Of Chlorella Vulgaris Cpcc 90, Anuradha Krishnan

Electronic Thesis and Dissertation Repository

Today, microalgae are cultured in large-scale systems such as open ponds and photobioreactors. Open pond systems, while not expensive, have disadvantages, including contamination and high water consumption, resulting in increased cost of harvesting and quick evaporation. Photobioreactors overcome some of the challenges of an open pond system. However, the reactor setup, operation, and microalgae harvesting are relatively more expensive when compared to conventional techniques. In this study, a novel foam-based photobioreactor system was applied to investigate both the cultivation and harvest of microalgae using different surfactant agents, i.e., rhamnolipids, Bovine Serum Albumin (BSA), saponin, and the commercial surfactant Pluronic f127 …


Comparative Evaluation Of The Feasibility Of Utilizing The Bakken Formation Oil And Gas Field Produced Water For Extraction Of Critical Minerals And Salts, With A Focus On The Inyan Kara Formation: An Analysis Of Salinity And Infrastructure, Md Jakaria, Kegang Ling, Josh Crowell, Lisa Holding Eagle, Claudius Epie Njie 2023 University of North Dakota

Comparative Evaluation Of The Feasibility Of Utilizing The Bakken Formation Oil And Gas Field Produced Water For Extraction Of Critical Minerals And Salts, With A Focus On The Inyan Kara Formation: An Analysis Of Salinity And Infrastructure, Md Jakaria, Kegang Ling, Josh Crowell, Lisa Holding Eagle, Claudius Epie Njie

Petroleum Engineering Student Publications

This study compares the salt concentration and mineral composition of water from the Bakken Formation and the Inyan Kara Formation to assess their suitability for salt/critical minerals extraction. The results reveal that the Bakken Formation exhibits significantly higher levels of dissolved solids, calcium, magnesium, and chloride compared to the Inyan Kara Formation, indicating its potential suitability for salt/critical elements extraction. Conversely, the Inyan Kara Formation water displays higher bicarbonate concentrations, which may limit its applicability in certain salt extraction processes. The Bakken Formation proves more viable for water production due to its existing oil and gas infrastructure and abundant produced …


The Effects Of Engineering Summer Camps On Middle And High School Students’ Engineering Interest And Identity Formation: A Multi-Methods Study, Timothy Robinson, Adam Kirn, Jenny Amos, Indira Chatterjee 2023 University of Nevada, Reno

The Effects Of Engineering Summer Camps On Middle And High School Students’ Engineering Interest And Identity Formation: A Multi-Methods Study, Timothy Robinson, Adam Kirn, Jenny Amos, Indira Chatterjee

Journal of Pre-College Engineering Education Research (J-PEER)

This multi-methods study explores changes in engineering interest and identity of middle and high school students (n = 79) attending introductory-level engineering summer camps at a large western land grant university. Middle school is a critical time when student interest, identity, and subsequently career choice begin to emerge and hence it is important that at this age students are given accurate information about engineering majors in college and future career opportunities in engineering. Data were collected over a period of two years in six summer camps. Three separate populations of middle and high school students participated in the summer …


Integration Of Algae And Biomass Processes To Synthesize Renewable Bioproducts For The Circular Economy, Jessica Martin 2023 University of South Florida

Integration Of Algae And Biomass Processes To Synthesize Renewable Bioproducts For The Circular Economy, Jessica Martin

USF Tampa Graduate Theses and Dissertations

Rapid population growth and global industrialization have substantially heightened the demand for fossil-based fuels and products in various sectors of the global economy, including energy production, transportation fuels, and as raw materials for petrochemicals. The intense consumption of fossil fuels has caused immense environmental impacts, especially pertaining to carbon dioxide emissions. Shifting to renewable feedstocks (raw materials) is expected to reduce these emissions by lowering the carbon footprint of fuels and products compared to traditional fossil-derived alternatives. This transition aligns with the goal of creating a sustainable and circular economy, emphasizing efficient resource use, and reducing waste generation through recycling …


Experimental Investigation Of The Vortex-Induced Vibration Response Of A Flexibly-Mounted Rigid Cylinder In The Shear-Thinning And Inertial-Viscoelastic Flow Regimes, Pieter Boersma 2023 University of Massachusetts Amherst

Experimental Investigation Of The Vortex-Induced Vibration Response Of A Flexibly-Mounted Rigid Cylinder In The Shear-Thinning And Inertial-Viscoelastic Flow Regimes, Pieter Boersma

Doctoral Dissertations

Flexible or flexibly-mounted structures with bluff cross-sections in flow can shed vortices at frequencies that increase with increasing flow velocity. When this shedding frequency is equal to the structure's natural frequency, the structure can oscillate. This is called vortex-induced vibrations (VIV). VIV is present in multiple fluid-structure interaction (FSI) systems which can be found in industrial, medical, and engineering applications. These oscillations can be desirable or undesirable, so understanding the physics behind this phenomenon is important. This work seeks to investigate experimentally the VIV response in the inertial-viscoelastic regime where fluid inertia and elasticity influence the system.

The subcritical Newtonian …


Optimizing Channel Formation In Peg Maleimide Hydrogels, Bakthavachalam Kannadasan 2023 University of Massachusetts Amherst

Optimizing Channel Formation In Peg Maleimide Hydrogels, Bakthavachalam Kannadasan

Masters Theses

Blood vessels including the arteries, veins, and capillaries are a critical and indispensable component of various organisms. Some studies estimate that if all the blood vessels present in our body are arranged in line, they would amount to a total length of approximately 60,000 miles. This distance is enough to circle the world two and a half times! In addition to being all pervasive, blood vessels perform certain key functions such as delivery of oxygen and nutrients to various tissues in the body. They also assist in the spread of diseases such as cancer. Therefore, it is important to study …


Electrostatic Effects On Lipid Bilayer Physicochemal Properties And Vesicle Adhesion, Oscar Zabala-Ferrera 2023 University of Massachusetts Amherst

Electrostatic Effects On Lipid Bilayer Physicochemal Properties And Vesicle Adhesion, Oscar Zabala-Ferrera

Doctoral Dissertations

Lipids are an integral part of cells, being the principal component of the cell membrane, and contributing to the function and regulation of biological processes. Lipid nanoparticles mimicking a cell’s endosomes or exosomes are of particular interest within the pharmaceutical industry for their ability to deliver cargo such as RNA into target cells. The delivery process faces a multitude of challenges, so a rational design approach for vesicles that considers a lipid’s physicochemical contribution to the membrane is desired. To that end, this thesis explores the creation of large area biomembranes along with the development of electromechanical and optical characterization …


Machine Learning Modeling Of Polymer Coating Formulations: Benchmark Of Feature Representation Schemes, Nelson I. Evbarunegbe 2023 University of Massachusetts Amherst

Machine Learning Modeling Of Polymer Coating Formulations: Benchmark Of Feature Representation Schemes, Nelson I. Evbarunegbe

Masters Theses

Polymer coatings offer a wide range of benefits across various industries, playing a crucial role in product protection and extension of shelf life. However, formulating them can be a non-trivial task given the multitude of variables and factors involved in the production process, rendering it a complex, high-dimensional problem. To tackle this problem, machine learning (ML) has emerged as a promising tool, showing considerable potential in enhancing various polymer and chemistry-based applications, particularly those dealing with high dimensional complexities.

Our research aims to develop a physics-guided ML approach to facilitate the formulations of polymer coatings. As the first step, this …


Carbonate Precipitation Behavior In Co2 Mea Electrolyzers With Cu Electrocatalyst, John C. Hendershot 2023 Louisiana State University

Carbonate Precipitation Behavior In Co2 Mea Electrolyzers With Cu Electrocatalyst, John C. Hendershot

LSU Master's Theses

Annually, over 35 billion metric tons of carbon dioxide (CO2) are emitted into Earth’s atmosphere. The accumulated CO2 emissions in the atmosphere over the last century drive climate change. CO2 electroreduction (CO2ER) is an emerging technology that aims to recycle carbon emissions from the industrial sector by using CO2, H2O, and electricity as a feedstock to produce ethylene (C2H4) at current densities as high as 1 A cm-2 and Faradaic efficiencies (FE) of 60%. However, CO2 electrolyzers currently demonstrate poor durability at industrially relevant …


Swelling Induced Buckling Of Photopatterned Hydrogel Beams, Chinar Yogesh Kokaje 2023 University of South Florida

Swelling Induced Buckling Of Photopatterned Hydrogel Beams, Chinar Yogesh Kokaje

USF Tampa Graduate Theses and Dissertations

The main goal of this study was to learn more about swelling induced buckling of surface-attached gel lines photo-patterned from thermally responsive polymers. A library of 10 different polymer compositions was synthesized using methacryloyloxybenzophenone (MABP) as the photo-cross-linking monomer. For each of the polymers, a series of beam-like patterns were prepared on polystyrene tissue culture dishes by first depositing a known amount of polymer into the tissue culture dish followed by pattern generation using a photo-lithography mask with line widths of 10, 20, 30, 50, and 80 μm. The target height of each polymer line was 100 μm. After development …


Comparative Evaluation Of Investigation Methods For Estimating The Load-Dependent State Of Charge And End Of Discharge Of A Multirotor Uav Battery, Hanna Dibbern, Morten Roßberg, Claudia Werner 2023 Flensburg University of Applied Sciences

Comparative Evaluation Of Investigation Methods For Estimating The Load-Dependent State Of Charge And End Of Discharge Of A Multirotor Uav Battery, Hanna Dibbern, Morten Roßberg, Claudia Werner

Journal of Aviation Technology and Engineering

As the scope of multirotor unmanned aerial vehicle (UAV) applications increases, more attention is being paid to UAV energy requirements, which vary depending on the mission profile. To obtain accurate information about the UAV battery during flight, the idea of a digital twin including a battery state estimation model is promising. For battery state estimation, a Kalman filter combination is the preferred approach in the literature. Comparing different Kalman filters, the unscented Kalman filter has a more accurate estimation for nonlinear systems compared to the extended Kalman filter. In the application of UAV flight with load-dependent flight missions, the comparison …


Stereoscopic-Based Mass Properties Estimation For Warhead Fragments, Alessia Nocerino, Katharine Larsen, Riccardo Bevilacqua, Elisabetta L. Jerome 2023 University of Naples Federico II

Stereoscopic-Based Mass Properties Estimation For Warhead Fragments, Alessia Nocerino, Katharine Larsen, Riccardo Bevilacqua, Elisabetta L. Jerome

Student Works

FRAGMENTATION characteristics such as spatial distribution, number of fragments, fragment velocity, and fragment mass can be used to characterize the lethality of a fragmenting weapon or any metal cased explosive [1,2]. However, most warhead tests and evaluations are limited to static arena testing, where fragment characteristics must be collected by hand. Recently, stereoscopic imaging techniques have been added to static arena tests. Using this method, position tracks can be collected for each fragment, and then velocity information can be found. This paper proposes a method to estimate the mass and moment of inertia using data collected by a stereoscopic imaging …


Computational Modeling Using A Novel Continuum Approach Coupled With Pathway-Informed Neural Networks To Optimize Dynein-Mediated Centrosome Positioning In Polarized Cells, Arkaprovo Ghosal, Padmanabhan Seshaiyar Dr., Adriana Dawes Dr., General Genomics Inc. 2023 George Mason University

Computational Modeling Using A Novel Continuum Approach Coupled With Pathway-Informed Neural Networks To Optimize Dynein-Mediated Centrosome Positioning In Polarized Cells, Arkaprovo Ghosal, Padmanabhan Seshaiyar Dr., Adriana Dawes Dr., General Genomics Inc.

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.


New Dual Modality Technique Of Gamma-Ray Densitometry (Grd) And Optical Fiber Probe (Ofp) To Investigate Line-Averaged Diameter Profiles Of Gas, Liquid, And Solid Holdups Along The Height Of A Slurry Bubble Column, Omar Farid, Binbin Qi, Sebastián Uribe, Muthanna H. Al-Dahhan 2023 Missouri University of Science and Technology

New Dual Modality Technique Of Gamma-Ray Densitometry (Grd) And Optical Fiber Probe (Ofp) To Investigate Line-Averaged Diameter Profiles Of Gas, Liquid, And Solid Holdups Along The Height Of A Slurry Bubble Column, Omar Farid, Binbin Qi, Sebastián Uribe, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

A new hybrid measurement technique was developed and employed to study line-averaged three-phase holdup distribution in a Slurry Bubble Column Reactor (SBCR) using gamma-ray densitometry (GRD) and point optical fiber probe (OFP) techniques. The OFP technique was used to measure the local gas holdup at 45 nodes in the cross-section area of the SBCR at three different axial levels. Thus, the GRD technique measured the spectrum of the SBCR for two different orientations simultaneously with the OFP technique at the same operating conditions to determine the solid holdup distribution radially and axially. The line-averaged solid holdup for 25% solids loading …


Development Of Eco-Friendly Chitosan-G-Polyacrylamide Preformed Particle Gel For Conformance Control In High-Temperature And High-Salinity Reservoirs, Reem Elaf, Ahmed Ben Ali, Mohammed Saad, Ibnelwaleed A. Hussein, Baojun Bai 2023 Missouri University of Science and Technology

Development Of Eco-Friendly Chitosan-G-Polyacrylamide Preformed Particle Gel For Conformance Control In High-Temperature And High-Salinity Reservoirs, Reem Elaf, Ahmed Ben Ali, Mohammed Saad, Ibnelwaleed A. Hussein, Baojun Bai

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Oil and gas extraction has become challenging nowadays due to the accompanying amount of excess produced water that results in poor recoverability of hydrocarbon, besides other environmental and economic isues. A recent and efficient technology for conformance control is the injection of preformed particle gels (PPGs), which results in a more practical production process. Nevertheless, existing treatments fail in high-temperature reservoirs, are extremely sensitive to salinity, and are hazardous. The characteristics of the designed PPG, such as mechanical strength and thermal durability, is mainly depend on their crosslinking method. Polysaccharides-based gels prepared by physical crosslinking are weaker than the ones …


Landscape Position And Cover Crops Affects Crop Yields In A Terrace-Tiled Field, Harpreet Kaur, Kelly A. Nelson, Gurbir Singh, Gurpreet Kaur, Katherine R. Grote 2023 Missouri University of Science and Technology

Landscape Position And Cover Crops Affects Crop Yields In A Terrace-Tiled Field, Harpreet Kaur, Kelly A. Nelson, Gurbir Singh, Gurpreet Kaur, Katherine R. Grote

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Cover Crops (CC) Provide Soil Ecosystem Benefits Including Reduced Soil Erosion, Improved Soil Health, and Nutrient Cycling. However, the Effect of CCs on Rotational Commodity Crop Grain Yields and Nutrient Uptake May Vary at Different Landscape Positions and under Different Soil Moisture Dynamics. the Objectives of This Study Were to Determine the Influence of CCs in a Terraced Field on Soil Moisture at Crop Emergence and Reproductive Stages of Corn (Zea Mays L) and Soybean (Glycine Max L. Merr.) Development, and Evaluate CCmix (Wheat (Triticum Aestivum L.), Radish (Raphanus Raphanistrum Subsp. Sativus), and Turnip (Brassica Rapa Subsp. Rapa)) and Cereal …


Dependence Of The Granulometric Composition Of Chalk During Granulation In A Turbulized Layer, N.A. Annaev, S.Kh. Nurmukhamedov, Zh.V. Sultonov, N.Sh. Khudoiberdieva 2023 Tashkent Institute of Chemical Technology, Address: 100011, Navoi Street, 32, Tashkent, Uzbekistan.

Dependence Of The Granulometric Composition Of Chalk During Granulation In A Turbulized Layer, N.A. Annaev, S.Kh. Nurmukhamedov, Zh.V. Sultonov, N.Sh. Khudoiberdieva

Chemical Technology, Control and Management

The article presents the results of an experimental study on chalk granulation in the turbulized layer of a turboblade granulator. The design of the apparatus combines the following sequential processes: grinding, granulating and compacting newly formed granules. In addition, this device has the ability to carry out both rotational and reciprocating motion. It has been established that preliminary grinding of the feedstock increases the efficiency of the granulation process and improves the quality of the finished product. In the experiments, four substances were used to create 2 astringent suspensions and the optimal combination and their ratios were determined. The experiments …


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