Direct Electrochemistry And Glucose Biosensing Of Glucose Oxidase-Gold Nanoparticles Composite Synthesized By Enzyme Method,
2014
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China;
Direct Electrochemistry And Glucose Biosensing Of Glucose Oxidase-Gold Nanoparticles Composite Synthesized By Enzyme Method, Fang He, Xiao-Li Qin, Ying-Chun Fu, Chao Chen, Qing-Ji Xie, Shou-Zhuo Yao
Journal of Electrochemistry
Glucose oxidase (GOx)-gold nanoparticles (AuNPs) composite was synthesized by the one-pot enzyme reaction in the mixture solution of NaAuCl4, GOx and glucose, and a Nafion/GOx-AuNPs/glassy carbon electrode was fabricated by the cast-coating method to examine the direct electrochemistry of GOx and the biosensing performance on this electrode. Such a GOx-AuNPs composite showed good direct-electrochemistry activity and bioactivity of GOx, probably because the enzyme-mediated AuNPs are close to the redox active centers of GOx. This enzyme electrode exhibited a linear amperometric response to glucose concentration (0.54 mmol·L-1) at -0.4 V (vs. SCE), and a detection limit of …
Interfacial Polymerization For Colorimetric Labeling Of Protein Expression In Cells,
2014
University of Kentucky
Interfacial Polymerization For Colorimetric Labeling Of Protein Expression In Cells, Jacob L. Lilly, Phillip R. Sheldon, Liv J. Hoversten, Gabriela Romero, Vivek Balasubramaniam, Brad J. Berron
Chemical and Materials Engineering Faculty Publications
Determining the location of rare proteins in cells typically requires the use of on-sample amplification. Antibody based recognition and enzymatic amplification is used to produce large amounts of visible label at the site of protein expression, but these techniques suffer from the presence of nonspecific reactivity in the biological sample and from poor spatial control over the label. Polymerization based amplification is a recently developed alternative means of creating an on-sample amplification for fluorescence applications, while not suffering from endogenous labels or loss of signal localization. This manuscript builds upon polymerization based amplification by developing a stable, archivable, and colorimetric …
Hydrodynamics Of Dna Confined In Nanoslits And Nanochannels,
2014
University of Minnesota - Twin Cities
Hydrodynamics Of Dna Confined In Nanoslits And Nanochannels, K. D. Dorfman, D. Gupta, A. Jain, Douglas R. Tree
Faculty Publications
Modeling the dynamics of a confined, semiflexible polymer is a challenging problem, owing to the complicated interplay between the configurations of the chain, which are strongly affected by the length scale for the confinement relative to the persistence length of the chain, and the polymer-wall hydrodynamic interactions. At the same time, understanding these dynamics are crucial to the advancement of emerging genomic technologies that use confinement to stretch out DNA and “read” a genomic signature. In this mini-review, we begin by considering what is known experimentally and theoretically about the friction of a wormlike chain such as DNA confined in …
Synthesis Of Au And Ag Catalysts With Controlled Sizes Of Metal Particles,
2014
University of South Carolina - Columbia
Synthesis Of Au And Ag Catalysts With Controlled Sizes Of Metal Particles, Eleni A. Kyriakidou
Theses and Dissertations
The performance of supported metal catalysts largely depends on the structure and composition of the metal particles and the nature of the support. In order to achieve better control over these parameters, synthetic route based on the use of templating agents has been proposed. Among the different templating agents, poly(amidoamine) (PAMAM) dendrimers have attracted a lot of recent attention as nanoparticle stabilizers. In this thesis, Au/G4OH nanocomposites were synthesized in aqueous solutions and used as precursors for the preparation of SiO2-supported Au nanoparticles. Elemental analysis, UV-Vis, and STEM measurements were used to estimate the extent of the Au-dendrimer interactions and …
Novel Biofabrication Technologies To Recapitulate In Vivo Geometries In Collagen Hydrogels,
2014
University of South Carolina - Columbia
Novel Biofabrication Technologies To Recapitulate In Vivo Geometries In Collagen Hydrogels, Veronica Rodriguez-Rivera
Theses and Dissertations
Tissue engineering and regenerative medicine aims to restore form and function to tissues that have been lost or damaged due to disease, congenital defect, or trauma. Biomaterials suitable to restore these complex tissues need to provide a balance between chemical and mechanical properties, providing accurate cell-matrix interaction and induce in vivo behaviors such as proliferation, differentiation and migration. It also requires that physical and chemical cues be presented to the body in the proper temporal and spatial pattern. The extracellular matrix exerts forces that are transmitted through focal adhesion causing changes in the cell behavior. The hypothesis for this dissertation …
Surface Modification Of Nanoplatelets In Polymer Nanocomposites,
2014
University of South Carolina - Columbia
Surface Modification Of Nanoplatelets In Polymer Nanocomposites, Shailesh K. Shori
Theses and Dissertations
In polymer nanocomposites (PNCs), it is very important that the nanofiller (typically an inorganic) is well-dispersed in an organic polymer matrix. Optimal compatibility results in end-use products such as packaging materials with enhanced properties. In practice, however, this is challenging to achieve because “ideal” dispersions require the complete uniform separation, termed exfoliation, of the nanofiller within the matrix. As a result, different nanofillers including calcium niobate (CN) and montmorillonite (MMT) are first studied for their ability to exfoliate in aqueous suspensions by measuring the organic cation uptake. The cation exchange capacity, or CEC, determined that CN needs further work before …
A Three-Dimensional-Engineered Matrix To Study The Microenvironment Of Cancer Stem Cells,
2014
University of South Carolina - Columbia
A Three-Dimensional-Engineered Matrix To Study The Microenvironment Of Cancer Stem Cells, Samaneh Kamali Sarvestani
Theses and Dissertations
Maintenance of cancer stem cells (CSCs) is regulated by their microenvironment. As cancer cells are affected by many factors in their microenvironment, a major challenge is to isolate the effect of a specific factor on CSCs while keeping other factors unchanged. We developed a synthetic inert 3D Poly Ethylene Glycol Di-Acrylate (PEGDA) gel culture system as a unique tool to study the effect of microenvironmental factors on CSCs response. Synthetic hydrogels provide the flexibility to design three-dimensional (3D) matrices to isolate and study individual factors in the tumor microenvironment. The first objective of this work was to investigate the effect …
Bio-Active Degradable Micellar Hydrogels For Osteogenic Differentiation Of Mesenchymal Stem Cells,
2014
University of South Carolina - Columbia
Bio-Active Degradable Micellar Hydrogels For Osteogenic Differentiation Of Mesenchymal Stem Cells, Seyedsina Moeinzadeh
Theses and Dissertations
A feasible approach to the restoration of injured bone tissue is the use of tissue engineering strategies to deliver mesenchymal stem cells (MSCs) to the site of regenration in degradable, in-situ gelling, bio-active hydrogels. Although inert Polyethylene glycol (PEG) hydrogels provide flexibility in controlling the cell microenvironment, their use for in vivo applications in tissue regeneration is limited by their persistence (nondegradability) in the site of regeneration. In an attempt to produce inert hydrolytically degradable matrices for encapsulation of MSCs, hydrogels based on star PEG macromonomers chain-extended with short hydroxy acid (HA) segments (SPEXA) were synthesized and characterized with respect …
Theoretical Investigation Of The Catalytic, Liquid-Phase Hydrodeoxygenation Of Organic Acids And Esters,
2014
University of South Carolina - Columbia
Theoretical Investigation Of The Catalytic, Liquid-Phase Hydrodeoxygenation Of Organic Acids And Esters, Sina Behtash
Theses and Dissertations
With worldwide fossil fuel resources dwindling and greenhouse gas emissions rising, it is urgent to find renewable liquid fuel alternatives from e.g. biomass to meet the world’s growing energy demand. Lipid feedstocks and pyrolysis oils from woody biomass can be utilized for the production of second-generation biofuels via a catalytic hydrodeoxygenation (HDO) process. The conversion of fatty acids and esters plays an important role in the activity and selectivity of these processes. Understanding the HDO reaction mechanism of organic acids and esters on metal surfaces is a prerequisite for the rational design of new HDO catalysts specifically designed for upgrading …
Quantifying Individual Losses In A Direct Methanol Fuel Cell,
2014
University of South Carolina - Columbia
Quantifying Individual Losses In A Direct Methanol Fuel Cell, Jennifer Rae Ruffing
Theses and Dissertations
The performance of a direct methanol fuel cell (DMFC) is complicated due to the complex interactions of kinetic and transport processes. As a result, changes in one aspect of the cell have consequences in other aspects, which are difficult to elucidate from the full-cell polarization (i.e. voltage vs. current) behavior that fuel cell researchers often use to characterize the performance of their systems. The objective of this work was to develop a strategy to use current and voltage relationships from anode half cells, cathode half-cells, and a hydrogen pump, coupled with methanol crossover data and a mathematical model, to quantify …
Reduced Astrocyte Viability At Physiological Temperatures From Magnetically Activated Iron Oxide Nanoparticles,
2014
Missouri University of Science and Technology
Reduced Astrocyte Viability At Physiological Temperatures From Magnetically Activated Iron Oxide Nanoparticles, Nicholas J. Schaub, Deniz Rende, Yuan Yuan, Ryan J. Gilbert, Diana Andra Borca-Tasciuc
Chemical and Biochemical Engineering Faculty Research & Creative Works
Superparamagnetic iron oxide nanoparticles (SPIONs) can generate heat when subjected to an alternating magnetic field (AMF). In the European Union, SPIONs actuated by AMF are used in hyperthermia treatment of glioblastoma multiforme, an aggressive form of brain cancer. Current data from clinical trials suggest that this therapy improves patient life expectancy, but their effect on healthy brain cells is virtually unknown. Thus, a viability study involving SPIONs subjected to an AMF was carried out on healthy cortical rat astrocytes, the most abundant cell type in the mammalian brain. The cells were cultured with amino silane- or starch-coated SPIONs with or …
Application Of Multivariate Analysis Techniques To Safeguards Of The Electrochemical Treatment Of Used Nuclear Fuel,
2014
Brigham Young University - Provo
Application Of Multivariate Analysis Techniques To Safeguards Of The Electrochemical Treatment Of Used Nuclear Fuel, Devin Rappleye, Sang-Mun Jeong, Michael Simpson
Faculty Publications
Several countries have shown interest in developing the electrochemical treatment of used nuclear fuel (UNF), commonly termed pyroprocessing. From a proliferation perspective, an advantage of pyroprocessing is its inability to isolate pure plutonium. However, because plutonium is present in the process, it needs to be effectively safeguarded to protect against diversion of plutonium-containing materials that could be post-processed elsewhere. The most complicated unit to safeguard in the process is the electrorefiner where UNF is chemically separated into several material entities. Molten LiCl–KCl serves as the electrolyte into which actinides (including uranium and plutonium), rare earths, and other active metals from …
Process Simulation Of Biomass Gasification Integrated With A Solid Oxide Fuel Cell Stack,
2014
Technological University Dublin
Process Simulation Of Biomass Gasification Integrated With A Solid Oxide Fuel Cell Stack, Wayne Doherty, Anthony Reynolds, David Kennedy
Articles
Biomass gasification-solid oxide fuel cell (BG-SOFC) combined heat and power (CHP) systems are of major interest in the context of climate change mitigation, energy security and increasing energy efficiency. Aspen Plus is employed to simulate various BG-SOFC CHP systems. The aim of the research work is to investigate the technical feasibility of these systems and to study the influence of important operating parameters and examine integration options. Systems based on dual fluidised bed steam gasification and tubular SOFC technologies are modelled. The cathode recycle and electric heater integration options are not attractive in comparison to the base case anode recycle …
Design Of Gan-Based Low-Loss Y-Branch Power Splitter,
2014
Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
Design Of Gan-Based Low-Loss Y-Branch Power Splitter, Retno Wigajatri Purnamaningsih, Irma Saraswati, Nji Raden Poespawati, Elhadj Dogheche
Makara Journal of Technology
We present a Y-branch power splitter design with a multimode section using GaN on Sapphire at telecommunication wavelength. The GaN sample optical properties were also investigated, resulting in a refractive index for the GaN layers nTE=2.289±0.001 and nTM=2.324±0.00. Optimization of the structure parameters for this structure was conducted accurately using BPM methods. The results demonstrated the possibility of realizing a GaN-based Y-branch power splitter for various applications.
Ultra-Wideband Notched Characteristic Fed By Coplanar Waveguide,
2014
Department of Electrical Engineering, Faculty of Industrial Technology, Universitas Trisakti, Jakarta Barat 11440, Indonesia
Ultra-Wideband Notched Characteristic Fed By Coplanar Waveguide, Rastanto Hadinegoro, Yuli Kurnia Ningsih, Indra Surjati
Makara Journal of Technology
In this paper, a novel Ultra-Wide Band (UWB) notch patch antenna with co-planar waveguide (CPW) fed is presented. This antenna only used one layer and the patch antenna is constructed on the first layer and back to back with CPW fed and bottom part is ground plane. The width notch is used to achieve the UWB characteristic. The results shown that the impedance bandwidth is 1130 MHz (1.662–2.792 GHz) or about 50.7% for VSWR <2.
Development Of The Soekarno–Hatta International Airport Rail Link Project Using The Value Engineering Method: Creating Value For Money,
2014
Department of Civil Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
Development Of The Soekarno–Hatta International Airport Rail Link Project Using The Value Engineering Method: Creating Value For Money, Mohammed Ali Berawi, Bambang Susantono, Suyono Dikun, Tommy Ilyas, Herawati Zetha Rahman, Abdur Rohim Boy Berawi, Teuku Yuri Zagloel, Perdana Miraj, Jade Sjafrecia Petroceany
Makara Journal of Technology
Soekarno–Hatta Airport is the main gateway for international flights to Greater Jakarta. Its accessibility depends on the inter-city and Sedyatmo toll roads, which causes congestion in peak hours, leading to uncertainty about travel times. The Soekarno–Hatta International Airport Rail Link (SHIARL) is proposed as an alternative mass transportation project, which is expected to provide accessibility and mobility for people and goods to and from the airport. Previously, the project was unattractive to private investors as it was technically and financially unfeasible. Therefore, this research aims to improve the feasibility of the Soekarno-Hatta International Airport Rail Link (SHIARL) by using a …
The Mechanical And Tribology Properties Of Sputtered Titanium Aluminum Nitride Coating On The Tungsten Carbide Insert Tool In The Dry Turning Of Tool Steel,
2014
Department of Physics, Faculty of Science and Mathematics, Universitas Negeri Jakarta, Jakarta 13220, Indonesia
The Mechanical And Tribology Properties Of Sputtered Titanium Aluminum Nitride Coating On The Tungsten Carbide Insert Tool In The Dry Turning Of Tool Steel, Esmar Budi, Mohd. Razali Bin Muhamad, Md. Nizam Bin Abdul Rahman
Makara Journal of Technology
The effect of the sputtering parameters on the mechanical tribology properties of Titanium Aluminum Nitride coating on the tungsten cabide insert tool in the dry turning of tool steel has been investigated. The coating was deposited using a Direct Current magnetron sputtering system with various substrate biases (-79 to -221 V) and nitrogen flow rates (30 to 72 sccm). The dry turning test was carried out on a Computer Numeric Code machine using an optimum cutting parameter setting. The results show that the lowest flank wear (~0.4 mm) was achieved using a Titanium Aluminum Nitride-coated tool that was deposited at …
Continuous Production Of Nanotubes And Carbon Clusters By Use Of The Revolver-Injection-Type Arc Jet Producer,
2014
Department of Physics, Shizuoka University, Shizuoka, 422-8529 Japan
Continuous Production Of Nanotubes And Carbon Clusters By Use Of The Revolver-Injection-Type Arc Jet Producer, Tetsu Mieno, Naoki Matsumoto, Takahashi Tomie, H. Inoue
Makara Journal of Technology
Efficient and continuous production of fullerenes, single-walled carbon nanotubes (SWNTs), and carbon nano-particles has been developed by the JxB arc jet method, where the Lorentz force of magnetic field jets out gaseous carbon particles to the JxB direction. In order to realize continuous mass production of these carbon clusters, the revolverinjection- type arc jet producer (RIT-AJP) has been developed. Production of fullerenes, endohedral metallo-fullerenes, SWNTs, metal-encapsulated carbon nano particles has been examined.
Analysis Of Vibration Generated By The Rubbing Of Flat Surfaces,
2014
Department of Mechanical Engineering, Universitas Syiah Kuala, Darussalam, Banda Aceh 23111, Indonesia
Analysis Of Vibration Generated By The Rubbing Of Flat Surfaces, Zahrul Fuadi
Makara Journal of Technology
Among the phenomena that occur as a result of contact between two surfaces is friction sound, in which surface roughness is of great importance. In this study, the parameter of contact stiffness is used to explain the frequency characteristic of vibration generated by the rubbing of flat surfaces under a small load. A 3-disk configuration rubbing method was used, which provides a unique characteristic of the system's frequency response function containing specific information about the rubbing vibrations. It is shown that the peak frequency of the rubbing vibration can be explained by using a two-degrees-of-freedom model incorporating the parameter of …
Modified Design Of Pin-On-Ring Tribometer For Hip Joint Prostheses Measurement; Case Study On Salat Activity,
2014
Industrial Technology Faculty, Mechanical Engineering Department, Universitas Islam Indonesia, Yogyakarta 55584, Indonesia
Modified Design Of Pin-On-Ring Tribometer For Hip Joint Prostheses Measurement; Case Study On Salat Activity, Muhammad Khafidh, Jamari Jamari, Sulardjaka Sulardjaka, Mohamad Tauviqirrahman, Emile Van Der Heide
Makara Journal of Technology
Total hip replacement (THR) is one of the most successful orthopedic surgical procedures for replacing a broken hip joint. In THR, wear may occur at the articulating surface of the acetabular cup and the femoral head. In Indonesia, the country with the largest Muslim population in the world, most of the inhabitants do salat (praying) every day. THR users are banned from doing salat for fear it will damage the hip joint prostheses. The previous wear calculation methods on the hip joint prostheses use the gravimetric, coordinate measuring machine (CMM), profiler, and geometric method. The disadvantages of the previous methods …
