Biomedical Engineering and Bioengineering Commons^™

The Effect Of Pulsed Field Dc Electrophoresis And Field Amplified Sample Stacking On The Microchip Electrophoretic Separation Of Organic Dyes, Travis Geoffrey Stewart

Theses and Dissertations

The utility of field amplified sample stacking and pulsed field electrophoresis toward improving electrophoretic outcomes was studied with regards to the electrophoretic separation of three organic dyes rhodamine B, 2’,7’-dichlorofluorescein, and fluorescein salt in microchip electrophoresis conditions. In this study, there were four experimental groups: nonstacking nonpulsed, nonstacking pulsed, sample stacking nonpulsed, and sample stacking pulsed.

Electrophoretic outcomes were evaluated by examining the electrophoretic separations under an epifluoresence detection method, plotting the signal intensities vs. time, and comparing separation resolutions and signal-to-noise ratios between experimental groups. From this it was shown that sample stacking nonpulsed conditions yielded the best outcome …

Vibrotactile Perception For Sensorimotor Augmentation: Perceptual Discrimination Of Vibrotactile Stimuli Induced By Low-Cost Eccentric Rotating Mass Motors At Different Body Locations In Young, Middle-Aged, And Older Adults, Ella Pomplun, Ashiya Thomas, Erin Corrigan, Valay A. Shah, Leigh A. Mrotek, Robert A. Scheidt

Biomedical Engineering Faculty Research and Publications

Sensory augmentation technologies are being developed to convey useful supplemental sensory cues to people in comfortable, unobtrusive ways for the purpose of improving the ongoing control of volitional movement. Low-cost vibration motors are strong contenders for providing supplemental cues intended to enhance or augment closed-loop feedback control of limb movements in patients with proprioceptive deficits, but who still retain the ability to generate movement. However, it remains unclear what form such cues should take and where on the body they may be applied to enhance the perception-cognition-action cycle implicit in closed-loop feedback control. As a step toward addressing this knowledge …

Electro-Mechanical Data Fusion For Heart Health Monitoring, Kemal Yakut

Theses and Dissertations

Heart disease is a major public health problem and one of the leading causes of death worldwide. Therefore, cardiac monitoring is of great importance for the early detection and prevention of adverse conditions. Recently, there has been extensive research interest in long-term, continuous, and non-invasive cardiac monitoring using wearable technology. Here we introduce a wearable device for monitoring heart health. This prototype consists of three sensors to monitor electrocardiogram (ECG), phonocardiogram (PCG), and seismocardiogram (SCG) signals, integrated with a microcontroller module with Bluetooth wireless connectivity. We also created a custom printed circuit board (PCB) to integrate all the sensors into …

Biomimetic Strategies To Control Therapeutic Release From Novel Dna Nanoparticles, Robert J. Mosley

Theses and Dissertations

The inherent chemical, mechanical, and structural properties of nucleic acids make them ideal candidates for the formulation of tunable, personalized drug nanocarriers. However, none so far have exploited these properties for the controlled release of therapeutic drugs. In this dissertation, a biomimetic approach to controlling drug release is exhibited by specifically manipulating the architecture of novel, DNA nanoparticles to take advantage of drug binding mechanisms of action. Rationally designed DNA strands were immobilized on gold surfaces via a terminal thiol modification. Immobilized monomers can be manipulated to form distinct monolayer architectures including flat, folded, coiled, or stretched structures. Increasing the …

Effectiveness Of Mesoporous Bioglass In Drug Delivery, Sheila Galbreath, Bailey Krueger, Taylor Frazier, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

Since the invention of bioactive glass 50 years ago, it has become a versatile material used in healthcare in a variety of applications and compositions. Bioactive glass has shown superior capabilities of drug delivery compared to traditional carriers. For example, time-released medications are less likely to reach toxic levels, while delivering a specific, therapeutic dose to a localized area. The objective of this paper is to investigate the properties and effectiveness of mesoporous bioglass (MBG) as a drug delivery carrier. A literature review of various polymer coated 45S5 Bioglass^® loaded with vancomycin was analyzed to determine their drug release …

Positive Rate-Dependent Action Potential Prolongation By Modulating Potassium Ion Channels, Candido Cabo

Publications and Research

Pharmacological agents that prolong action potential duration (APD) to a larger extent at slow rates than at the fast excitation rates typical of ventricular tachycardia exhibit reverse rate dependence. Reverse rate dependence has been linked to the lack of efficacy of class III agents at preventing arrhythmias because the doses required to have an anti-arrhythmic effect at fast rates may have pro-arrhythmic effects at slow rates due to an excessive APD prolongation. In this report we show that, in computer models of the ventricular action potential, APD prolongation by accelerating phase 2 repolarization (by increasing I_Ks) and decelerating …

Hybrid Rans-Les Hemolytic Power Law Modeling Of The Fda Blood Pump, Joseph Tarriela

USF Tampa Graduate Theses and Dissertations

This study applies the SBES and IDDES hybrid RANS-LES turbulence models along with the K-ω SST model to four flow conditions of the FDA blood pump. Validation of all three turbulence models show good agreement with experimental pressure and velocity fields. Evaluation of turbulent kinetic energy fields for the hybrid models show 80-90+% of kinetic energy is resolved in the rotor and diffuser regions of the pump. Hemolysis power law models were evaluated using the commonly used von Mises stress and additional energy dissipation stress (EDS). Results show viscous and Reynolds stresses computed with the K-ω SST under predict and …

An Improved Polynomial Chaos Expansion Based Response Surface Method And Its Applications On Frame And Spring Engineering Based Structures, Mhd A. Hafez

ETD Archive

In engineering fields, computational models provide a tool that can simulate a real world response and enhance our understanding of physical phenomenas. However, such models are often computationally expensive with multiple sources of uncertainty related to the model’s input/assumptions. For example, the literature indicates that ligament’s material properties and its insertion site locations have a significant effect on the performance of knee joint models, which makes addressing uncertainty related to them a crucial step to make the computational model more representative of reality. However, previous sensitivity studies were limited due to the computational expense of the models. The high computational …

Design And Finite Element Analysis Of Patient-Specific Total Temporomandibular Joint Implants, Shirish M. Ingawale, Tarun Goswami

Biomedical, Industrial & Human Factors Engineering Faculty Publications

In this manuscript, we discuss our approach to developing novel patient-specific total TMJ prostheses. Our unique patient-fitted designs based on medical images of the patient’s TMJ offer accurate anatomical fit, and better fixation to host bone. Special features of the prostheses have potential to offer improved osseo-integration and durability of the devices. The design process is based on surgeon’s requirements, feedback, and pre-surgical planning to ensure anatomically accurate and clinically viable device design. We use the validated methodology of FE modeling and analysis to evaluate the device design by investigating stress and strain profiles under functional/normal and para-functional/worst-case TMJ loading …

Zein And Lignin-Graft-Plga Nanoparticles As Agrochemical Delivery Systems To Soybean Plants, Eban A. Hanna

LSU Master's Theses

The majority of applied agrochemicals are lost to the environment due to their poor adhesion to plant tissues, resulting in multiple applications and subsequent environmental toxicity. This thesis focuses on reducing these consequences by entrapping agrochemicals in polymeric nanoparticles (PNPs). In the first section, zein nanoparticles (ZNPs) with entrapped methoxyfenozide (MFZ) (209.0 ± 5.6 nm, -42.1 ± 2.1 mV) were synthesized and used to facilitate the translocation of MFZ to the leaves and stems of soybean plants, as measured by LC-MS, under hydroponic conditions. The agrochemical concentration increased within the leaves over time from 0.04 to 2.35 μg/g at 0.2 …

Examining Metal Contents In Primary And Secondhand Aerosols Released By Electronic Cigarettes, Kashala Fabrice Kapiamba, Weixing Hao, Stephen Adom, Wenyan Liu, Yue-Wern Huang, Yang Wang

Chemistry Faculty Research & Creative Works

The usage of electronic cigarettes (ECs) has surged since their invention two decades ago. However, to date, the health effects of EC aerosol exposure are still not well understood because of insufficient data on the chemical composition of EC aerosols and the corresponding evidence of health risks upon exposure. Herein, we quantified the metals in primary and secondhand aerosols generated by three brands of ECs. By combining aerosol filter sampling and inductively coupled plasma mass spectrometry (ICP-MS), we assessed the mass of metals as a function of EC flavoring, nicotine concentration, device power, puff duration, and aging of the devices. …

Functional Requirements For A Samd14-Capping Protein Complex In Stress Erythropoiesis, Suhita Ray, Linda Chee, Yichao Zhou, Meg A. Schaefer, Michael J. Naldrett, Sophie Alvarez, Nicholas T. Woods, Kyle J. Hewitt

Nebraska Center for Biotechnology: Faculty and Staff Publications

Acute anemia induces rapid expansion of erythroid precursors and accelerated differentiation to replenish erythrocytes. Paracrine signals—involving cooperation between stem cell factor (SCF)/Kit signaling and other signaling inputs—are required for the increased erythroid precursor activity in anemia. Our prior work revealed that the sterile alpha motif (SAM) domain 14 (Samd14) gene increases the regenerative capacity of the erythroid system in a mouse genetic model and promotes stress-dependent Kit signaling. However, the mechanism underlying Samd14’s role in stress erythropoiesis is unknown. We identified a protein-protein interaction between Samd14 and the α- and β-heterodimers of the F-actin capping protein (CP) complex. Knockdown of …

Exploring The Metabolic Landscape Of Pancreatic Ductal Adenocarcinoma Cells Using Genome-Scale Metabolic Modeling, Mohammad Mazharul Islam, Andrea Goertzen, Pankaj K. Singh, Rajib Saha

Department of Chemical and Biomolecular Engineering: Faculty Publications

Pancreatic ductal adenocarcinoma (PDAC) is a major research focus because of its poor therapy response and dismal prognosis. PDAC cells adapt their metabolism to the surrounding environment, often relying on diverse nutrient sources. Because traditional experimental techniques appear exhaustive to find a viable therapeutic strategy, a highly curated and omics-informed PDAC genome-scale metabolic model was reconstructed using patient-specific transcriptomics data. From the model-predictions, several new metabolic functions were explored as potential therapeutic targets in addition to the known metabolic hallmarks of PDAC. Significant downregulation in the peroxisomal beta oxidation pathway, flux modulation in the carnitine shuttle system, and upregulation in …

Microheater Stimulation Platform For Neural Cells, Haley Royce

USF Tampa Graduate Theses and Dissertations

Electrical stimulation is the basis of many biomedical devices like cochlear implants,pacemakers, and retinal implants. In these systems, current is applied to a desired tissue to effectively stimulate it. Unfortunately, this is not localized as the current will spread through the tissue, limiting the spatial resolution. Thus, alternative forms of stimulation are needed to improve device precision and resolution. This project explores microheaters as a stimulation platform that can be easily integrated with existing biomedical devices. Microheaters produce heat via the Joule heating effect wherein electrical current is run through high resistance materials to output thermal energy. They are highly …

Cardio-Net: A Matlab-Based Software For The Display And Diagnostic Utilization Of Vectorcardiograms, Ali H. Mannaa, Domenico Gatti

Medical Student Research Symposium

Background: The 12-lead technique is the standard in ECG, however alternate cardiography modalities such as vectorcardiography (VCG) exist . While the VCG modality offers unique clinical metrics and certain advantages over ECG, it is hardly utilized due to it being more difficult to obtain than ECG. Here we introduce Cardio-Net, a MATLAB-based software that uses standard 12-lead ECG data to generate and visualize VCGs. Furthermore, we demonstrate the diagnostic potential of VCG by utilizing a recurrent neural network (RNN) to accurately classify vectorcardiograms.

Methods: MATLAB version 2019b and the following toolboxes were used for data processing: Deep learning, …

Antimicrobial Mechanisms Of Biomaterials: From Macro To Nano, Shounak Roy, Sanchita Sarkhel, Deepali Bisht, Samerender Nagam Hanumantharao, Smitha Rao, Amit Jaiswal

Michigan Tech Publications, Part 1

Overcoming the global concern of antibiotic resistance is one of the biggest challenge faced by scientists today and the key to tackle this issue of emerging infectious diseases is the development of next-generation antimicrobials. The rapid emergence of multi-drug resistant microbes, superbugs and mutated strains of viruses have fueled the search for new and alternate antimicrobial agents with broad-spectrum biocidal activity. Biomaterials, ranging from macroscopic polymers, proteins, and peptides to nanoscale materials such as nanoparticles, nanotubes and nanosheets have emerged as effective antimicrobials. An extensive body of research has established the antibacterial and antiviral efficiencies of different types of biomaterials. …

A Novel Electroconductive Nanofibrous Scaffold For Bone Regeneration, Mitchell Kenter

Medical Engineering Theses

The goal of this study was to develop a biodegradable and conductive scaffold to mimic the piezoelectric properties of bone and the architecture of the extracellular matrix. Poly(3,4- ethylenedioxythiophene) (PEDOT) is a conductive polymer of great interest in tissue engineering due to excellent electrical stability and biocompatibility. To enhance its conductivity, dopants such as dimethyl sulfoxide (DMSO) can be added. Engineered graphene oxide (GO) can also be introduced as oxidant to enhance conductivity and mechanical properties. PEDOT nanocomposites were synthesized by oxidative polymerization of 3, 4-Etylenedioxythiophene monomer (EDOT) in the presence of GO, DMSO, ferric chloride and various solvents. The …

Minimally Invasive 3d Printed Microneedles For Glucose Monitoring, Eduardo Quintero, Justin Wong

Bioengineering Senior Theses

Continuous glucose monitoring (CGM) provides an instantaneous real-time display of glucose level, rate of change of glucose, alerts, and alarms for actual or impending hypo- and hyperglycemia. Continuous glucose monitors are able to stay implanted on the patient anywhere from 7-14 days, giving patients and doctors valuable information to help improve patient care for diabetics, and providing them with a way to continuously monitor their blood glucose level concentration throughout the day.

Conventional methods of glucose detection such as blood glucose meters require the patient to gather frequent blood samples to generate instantaneous results that are only accurate as of …

Towards Targeted Delivery To Cns Neurons By Conquering The Bbb, David G.A. Bengford

Bioengineering Master's Theses

Brain cancer treatments have long been restricted by the blood-brain barrier (BBB). Semi-permeable in the most selective way, this barrier may be crossed by one of three methods: membrane integrity disruption, passive transport, or active transport. The third option offers the opportunity for many medically relevant cargoes, such as proteins and nucleic acids, to be shuttled into the brain. The concept of a therapeutic “Trojan horse” builds on this capability by employing engineered nanovesicles—in this work, engineered exosomes—whose surfaces are studded with ligands functioning as the keys to crossing the BBB. Exosomes are a powerful methodology for this approach because …

Immuno-Anti-Infective Drug Design Using Bioai, Cathy Chen, Vivian Zhang

Bioengineering Senior Theses

According to the World Health Organization, antibiotic resistance is one of the biggest threats to global health, food security, and development today. A growing number of infections, like Methicillin-resistant Staphylococcus aureus, are becoming harder to treat as the antibiotics used to treat them become less effective. As a result, the primary concern for infections in the hospital setting is due to the S. aureus’s growing resistance to antibiotics. Therefore, in response to this global health threat, our project focuses on furthering the research in developing a drug that S. aureus will not develop resistance to. In this paper, we assess …