Alkaloid Production By Hairy Root Cultures,
2014
Utah State University
Alkaloid Production By Hairy Root Cultures, Bo Zhao
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
In the present research, nicotine alkaloid production by Nicotiana tabacum (tobacco) hairy roots and tropane alkaloid production by Hyoscyamus niger hairy roots were investigated. The first objective of this research was to improve the oxygen mass transfer in hairy root cultures with microbubbles. Oxygen was shown as a critical nutrient for the growth of tobacco and H. niger hairy roots. In a 1-liter fermentor, microbubble dispersion improved the oxygen mass transfer, tobacco hairy root growth, and nicotine production in the medium. In a novel ground-joint column bioreactor, microbubbles enhanced the oxygen mass transfer and the growth of H. niger hairy …
Metabolic Modeling Of Spider Silk Production In E. Coli,
2014
Utah State University
Metabolic Modeling Of Spider Silk Production In E. Coli, Sarah Allred
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Spider silk has the potential to be a useful biomaterial due to its high tensile strength and elasticity. It is also biocompatible and biodegradable, making it useful for wound dressings and sutures, tissue and bone scaffolds, vessels for drug delivery, and ligament and tendon replacements. In some studies where spider silk has been used to grow cells, the silk has promoted more cell growth than the control. However, it is difficult to obtain the high volume of silk needed for these undertakings on a large scale. Spiders are territorial and cannibalistic, so they cannot be easily farmed. Therefore, spider silk …
Effect Of Poultry Litter Biochar On Saccharomyces Cerevisiae Growth And Ethanol Production From Steam-Exploded Poplar And Corn Stover,
2014
Utah State University
Effect Of Poultry Litter Biochar On Saccharomyces Cerevisiae Growth And Ethanol Production From Steam-Exploded Poplar And Corn Stover, Oumou Diallo
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The use of ethanol produced from lignocellulosic biomass for transportation fuel offers solutions in reducing environmental emission and the use of non-renewable fuels. However, lignocellulosic ethanol production is still hampered by economic and technical obstacles. For instance, the inhibitory effect of toxic compounds produced during biomass pretreatment was reported to inhibit the fermenting microorganisms, hence there was a decrease in ethanol yield and productivity. Thus, there is a need to improve the bioconversion of lignocellulosic biomass to ethanol in order to promote its commercialization. The research reported here investigated the use of poultry litter biochar to improve the ethanol production …
Distribution Of Heavy Metals From Flue Gas In Algal Bioreactor,
2014
Utah State University
Distribution Of Heavy Metals From Flue Gas In Algal Bioreactor, Katerine Napan
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Algae are microscopic organisms with a great potential to produce biomass and lipids at productivities several times higher than terrestrial crops. To grow, these organisms consume carbon dioxide (CO2), a greenhouse gas. This gas, emitted primarily by power plants after coal burning, can be effectively used for algae production, thus resulting in CO2 remediation and biomass beneficial utilization as feedstuff, industrial filler and biodiesel feedstock. However, since coal is a fuel mined from the earth’s crust, it contains heavy metals that are released during coal burning and inevitably enter the algal cultivation system, contaminating the water were …
Excimer-Monomer Switching Molecular Beacon: The Study On Synthetic Cryptosporidium Dna Detection, Thermodynamics, And Magnesium Effects,
2014
Utah State University
Excimer-Monomer Switching Molecular Beacon: The Study On Synthetic Cryptosporidium Dna Detection, Thermodynamics, And Magnesium Effects, Michael L. Davis
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The purpose of this study is to quantify and determine the binding of a molecular DNA probe or molecular beacon used in the detection of a known waterborne pathogen Cryptosporidium parvum. The objectives of this study are to determine the stability, sensitivity, and selectivity of the molecular beacon in detecting synthesized Cryptosporidium DNA sequences. The designed probe is based on a two-fluorophore system that is different than similar molecular beacons that utilize a fluorophore and quencher detection method. A comparative study between the two probes was also performed to demonstrate enhanced selectivity of the fluorophore based molecular beacon. Magnesium …
Mechanical And Physical Properties Of Spider Silk Films Made From Organic And Water-Based Dopes,
2014
Utah State University
Mechanical And Physical Properties Of Spider Silk Films Made From Organic And Water-Based Dopes, Chauncey Lewis Tucker
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Spider silk has become a popular material due to its exceptional mechanical and biocompatibility properties. The project team has developed methods of producing and improving the properties of synthetic spider silk films. Other groups have been able to produce similar films having less than impressive mechanical properties using a toxic solvent. The spider silk team here at Utah State University has developed a new way of processing these films to more than triple their mechanical properties and has created a novel form of spider silk films produced from a water-based liquid, making it less expensive, more environmentally friendly, and more …
Cryoprotectant Loading In A Microchannel,
2014
Louisiana State University
Cryoprotectant Loading In A Microchannel, Shelby Pursley
Honors Capstones
No abstract provided.
In Vivo Evaluation Of An Automated Pressure Sore Reducer,
2014
Louisiana State University
In Vivo Evaluation Of An Automated Pressure Sore Reducer, Linda J. Cross
Honors Capstones
No abstract provided.
Computational Modeling Of Drug Release From Bone Scaffolds,
2014
Louisiana State University
Computational Modeling Of Drug Release From Bone Scaffolds, Corey Landry
Honors Capstones
No abstract provided.
Investigating The Shelf Life Of Benzoyl Peroxide Coated Nozzles For Bone Cement Application,
2014
Syracuse University
Investigating The Shelf Life Of Benzoyl Peroxide Coated Nozzles For Bone Cement Application, Alysen Leigh Demzik
Renée Crown University Honors Thesis Projects - All
Two-solution bone cements (TSBC) were developed to address the limitations of current powder-liquid bone cements, however are characterized by a limited shelf life due to spontaneous free radical polymerization. As a solution to pre-polymerization concerns, the initiator benzoyl peroxide (BPO) was removed from the cement solution and incorporated into a thin film coating within the static mixing nozzle, allowing the BPO to integrate with the TSBC as it is mixed for use. Only short-term storage of BPO-coated nozzles and the subsequent effects on bone cement properties is known. The goal of this study was to investigate the effects of time, …
Modeling The Adaptive Immune Response To Mutation-Generated Antigens,
2014
University of Connecticut - Storrs
Modeling The Adaptive Immune Response To Mutation-Generated Antigens, Rory J. Geyer
Honors Scholar Theses
Somatic mutations may drive tumorigenesis or lead to new, immunogenic epitopes (neoantigens). The immune system is thought to represses neoplastic growths through the recognition of neoantigens presented only by tumor cells. To study mutations as well as the immune response to mutation-generated antigens, we have created a conditional knockin mouse line with a gene encoding, 5’ to 3’, yellow fluorescent protein (YFP), ovalbumin (which is processed to the immunologically recognizable peptide, SIINFEKL), and cyan fluorescent protein (CFP), or, YFP-ovalbumin-CFP. A frame shift mutation has been created at the 5’ end of the ovalbumin gene, hence YFP should always be expressed, …
Bioactivity And Cell-Mediated Targeting Of Multistage Nanoporous Silicon Particles,
2014
The University of Texas Graduate School of Biomedical Sciences at Houston
Bioactivity And Cell-Mediated Targeting Of Multistage Nanoporous Silicon Particles, Jonathan O. Martinez
Dissertations and Theses (Open Access)
Progress in drug delivery approaches have not adequately translated into clinical advances in the diagnosis or treatment of inflammatory disorders (e.g., cancer). This disconnect is rooted in the inefficient delivery of imaging and therapeutic agents to the inflamed site upon systemic delivery. A multitude of biological barriers pose insurmountable obstacles limiting the ability of the agent to effectively reach and accumulate at the target site. Nanoparticles (NP) surfaced as potential vectors to encapsulate and deliver biological agents. However, even after surface decoration, NP have failed to evade biological barriers (i.e., MPS) and to accumulate at the tumor site at therapeutic …
Application Of Limited Mixing In The Hele–Shaw Geometry In Fabrication Of Janus Hydrogels,
2014
University of Nebraska-Lincoln
Application Of Limited Mixing In The Hele–Shaw Geometry In Fabrication Of Janus Hydrogels, Md Mahmudur Rahman
Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research
It is widely accepted that cells behave differently responding to the stiffness of their extracellular matrix (ECM). Such observations were made by culturing cells on hydrogel substrates of tunable stiffness. However, it was recently proposed that cells may sense how strongly they are tethered to ECM, not the local stiffness of ECM. To investigate both hypotheses, we developed a method to fabricate Janus polyacrylamide (PAAM) gels. We squeeze two drops of different concentrations in the Hele-Shaw geometry to generate radial Stokes flow. When the drops coalesce, limited mixing occurs at the interface due to the narrow confinement, and diffusion normal …
Department Of Biological Systems Engineering Newsletter, April 2014,
2014
University of Nebraska - Lincoln
Department Of Biological Systems Engineering Newsletter, April 2014
BSE Department Magazine
No abstract provided.
Pretreatment Of Scenedesmus Sp. Biomass As A Potential Anaerobic Digestion Substrate,
2014
University of Kentucky
Pretreatment Of Scenedesmus Sp. Biomass As A Potential Anaerobic Digestion Substrate, Xinyi E, Czarena Crofcheck
Biosystems and Agricultural Engineering Faculty Publications
Algal biomass can be a potential substrate for anaerobic digestion. However, raw algae cells show a resistance to biological degradation, resulting in a slower methane production rate. Varying thermal and chemical pretreatments of algal biomass were investigated in an attempt to increase soluble organic matter (SOM) yield, which would result in enhanced methane production during subsequent anaerobic digestion. Scenedesmus sp. was harvested using three different procedures: with flocculation, with flocculation and drying, and without flocculation or drying. For all pretreatments and algae types, fluorescence micrographs were obtained to visually confirm the degradation of the algal cell walls. A complete 2 …
Developing Genetic Tools For Geobacillus,
2014
University of New Hampshire - Main Campus
Developing Genetic Tools For Geobacillus, Brittany Artale
Honors Theses and Capstones
Bacillus and Geobacillus are the primary bacterium used in biotechnology industries due to their ability to excrete extracellular materials such as proteins, enzymes and other byproducts. Bacillus subtilis, has a well-characterized competence machinery, giving way to characterized genetic tools. However, a significant problem associated with working with Bacillus is the regulation of endospore formation. An alternative to Bacillus is Geobacillus, a thermophilic bacterium. Thermophiles offer significant advantages over other bacteria as host organisms in biofuel, bioremediation, and biocatalyst processes. However, the genetic tools and competency associated with Geobacillus is still unknown. Through, genetic engineering the ability …
Evaluation Of Tissue-Engineered Tendon Enthesis Polymer Constructs,
2014
The University of Akron
Evaluation Of Tissue-Engineered Tendon Enthesis Polymer Constructs, Joshua A. Bundy Bs, Mary Beth Wade Phd, Hitomi Nakao Md, Phillip Mcclellan Phd, Qing Yu Phd, Robin Jacquet-Childs Ms, William J. Landis Phd
Williams Honors College, Honors Research Projects
Both scientists and clinicians have proposed tissue engineering as the future of medicine. The possibilities for tissue engineering, that is, fabrication of tissues and organs in the laboratory and their translation to patients, appear to be endless, and many believe that this new approach in medicine will result in abolishing many common ailments, injuries, and congenital defects. Injuries to a tendon enthesis, the normal tissue connection between tendon and bone, are of particular concern to clinicians because of their frequency and failure to repair as a result of surgery. While these injuries may not be life threatening, they can certainly …
Friction Coefficients For Dried Distillers Grains On Eight Structural Surfaces,
2014
Utah State University
Friction Coefficients For Dried Distillers Grains On Eight Structural Surfaces, G. C. Nyendu, S. Pflum, P. Schumacher, C. J. Bern, T. J. Brumm
Biological Engineering Faculty Publications
Static and dynamic coefficients of friction on structural surfaces play important roles in the power requirements and material selection for equipment used in handling and storing agricultural commodities. However, friction data on dried distillers grains with solubles (DDGS) is limited. Further, lack of a standardized method for determining friction coefficient on grain handling materials presents a challenge. This article describes studies carried out to determine the static coefficient of friction (•_s) and dynamic coefficient of friction (•_d) for corn DDGS at 10%, 8.2%, and 6.5% moisture content (all moistures are % wet basis) on eight structural surfaces: High-Density Polyethylene (HDPE), …
Temperature-Dependent Dielectric And Thermal Properties Of Whey Protein Gel And Mashed Potato,
2013
University of Nebraska-Lincoln
Temperature-Dependent Dielectric And Thermal Properties Of Whey Protein Gel And Mashed Potato, Jiajia Chen, Krishnamoorthy Pitchai, Sohan Birla, Ricardo Gonzalez, David Jones, Jeyamkondan Subbiah
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Temperature-dependent dielectric properties (dielectric constant and dielectric loss factor) and thermal properties (thermal conductivity and specific heat capacity) of whey protein gel and mashed potato were measured from -20°C to 100°C. A dielectric properties measurement system and a multipoint temperature calibration protocol were developed. The system consists of an impedance analyzer, a high-temperature coaxial cable, a high-temperature coaxial probe, a micro-climatic chamber and a metal sample holder. Calibrations at two temperatures (25°C and 85°C) were sufficient to accurately measure the dielectric properties of foods from frozen to hot temperatures. Both dielectric constant and dielectric loss factor rapidly increased from -20°C …
Conventional And Catalytic Pyrolysis Of Pinyon Juniper Biomass,
2013
Utah State University
Conventional And Catalytic Pyrolysis Of Pinyon Juniper Biomass, Bhuvanesh Kumar Yathavan
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Pinyon and juniper are invasive woody species which has occupied more than 47 million acres of land in Western United States. Pinyon juniper woodlands domination decreases the herbaceous vegetation, increase bare lands which in turn increases soil erosion and nutrition loss. Thus, The US Bureau of Land Management (BLM) has focused on harvesting these woody species to make room for herbaceous vegetation. The major application of harvested pinyon-juniper (PJ) is low value firewood. Thus, there is a need to develop new high value products from this woody biomass to reduce the cost of harvesting. In this study pyrolysis was carried …
