Biological Engineering Commons^™

Development Of A Protocol To Measure Gene Expression In The Mouse Tibia, Daniel Hoover

Biomedical Engineering

Numerous molecular factors active in bone tissue direct fracture repair and remodeling which can be altered by disease conditions such as Peripheral Arterial Disease (PAD) and Osteoporosis. Methods of molecular biology are commonly applied to investigate the expression and role of these molecular factors. This project presents a robust three-step protocol for examining gene expression in the mouse tibia. The protocol begins with isolating RNA from a flash frozen tibia sample. The isolated RNA is reverse transcribed into cDNA. Finally, PCR is performed to indentify expressed genes. Establishing this protocol will allow further research into the mechanisms of bone remodeling …

Computational Design And Characterization Of A Temperature-Sensitive Plasmid Replicon For Gram Positive Thermophiles, Daniel G. Olson, Lee R. Lynd

Dartmouth Scholarship

Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, Clostridium thermocellum. Traditional mutagenesis techniques yield Ts mutants at a low frequency, and therefore requires the development of high-throughput screening protocols, which are also not available for this organism. Recently there has been progress in the development of computer algorithms which can predict Ts mutations. Most plasmids currently used for genetic modification of C. thermocellum are based on the replicon of plasmid pNW33N, which replicates using the RepB replication protein. To address this problem, we set out …

Engineering Design Of A Prototype Ammonia Absorption Tower, Andrew Warren Calogero

Honors Capstones

No abstract provided.

Design And Implementation Of A Shoulder Simulator, Chelsea Lynn Kowal

Renée Crown University Honors Thesis Projects - All

The main objective of this thesis project is to build a shoulder simulator to be able to understand the mechanics of the shoulder, specifically the rotator cuff and deltoid muscles. The shoulder simulator should be able to mimic the important motions of the human shoulder by pulling on tendons of cadaver shoulders and moving the shoulder in various specified motions. In my thesis project, I worked on creating a shoulder simulator that would control the rotator cuff muscles along with the three deltoid muscles. In order to do this, I needed to design and test the shoulder simulator using cadaver …

Fluidized-Bed Gasification Of Dairy Manure By Box–Behnken Design, Hanjing Wu, Milford A. Hanna, David D. Jones

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Application of excessive animal manure to the land may cause some environmental problems such as eutrophication of surface waters, degradation of ground water quality, and threats to human health. This paper reports an experimental study on the technology of biomass gasification to treat animal waste by analyzing the effects of key operating parameters on gasification. In this research, dairy manure from the University of Nebraska dairy farm was first collected and dried, and then gasified in a fluidized-bed, laboratory-scale gasifier to generate syngas. The effects of three parameters, namely temperature, steam to biomass ratio (SBR) and the equivalence ratio (ER), …

Aptamer-Based Spr Biosensor For Detection Of Avian Influenza Virus, Hua Bai

Graduate Theses and Dissertations

Rapid and specific detection of avian influenza (AI) virus is urgently needed with the concerns over the outbreaks of highly pathogenic H5N1 avian influenza in animal and human infection. Aptamers are artificial oligonucleic acid that can specifically bind to target molecules. They show comparable affinity for target virus and better thermal stability than monoclonal antibodies. Those advantages make aptamers promising candidates in diagnostic and detection applications. The goal of this research was to use DNA&ndashaptamer as the specific recognition element in a portable surface plasmon resonance (SPR) biosensor for detection of AI H5N1 virus in poultry.

A SPR biosensor was …

Real-Time Face Detection And Recognition, Parker Hill, Brendan Morris

College of Engineering: Graduate Celebration Programs

The face has become a popular biometric for identification due to the wide range of features and difficulty in manipulation of the metric. In order to work towards a robust facial recognition system, this work contains a foundation for using the face as a recognition metric. First, faces are detected from still images using a Viola-Jones object detection algorithm. Then, Eigenfaces is applied to the detected faces. The system was tested on face databases as well as real-time feed from a web camera.

Department Of Biological Systems Engineering Newsletter, Volume 8, Number 1, April 2012

BSE Department Magazine

No abstract provided.

Accounting For Indirect Land-Use Change In The Life Cycle Assessment Of Biofuel Supply Chains, Susan Tarka Sanchez, Jeremy Woods, Mark Akhurst, Matthew Brander, Michael O'Hare, Terence P. Dawson, Robert Edwards, Adam J. Liska, Rick Malpas

Adam Liska Papers

The expansion of land used for crop production causes variable direct and indirect greenhouse gas emissions; and other economic, social and environmental effects. We analyze the use of life cycle analysis (LCA) for estimating the carbon intensity of biofuel production from indirect land-use change (ILUC). Two approaches are critiqued; direct, attributional life cycle analysis (ALCA) and consequential life cycle analysis (CLCA). A proposed hybrid “combined model” of the two approaches for ILUC analysis relies on first defining the system boundary of the resulting full LCA. Choices are then made as to the modeling methodology (economic equilibrium or cause-effect), data inputs, …

First Long-Term, Direct Measurements Of Evapotranspiration And Surface Water Balance In The Nebraska Sandhills, David P. Billesbach, Timothy J. Arkebauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The Nebraska SandHills is a vast grassland ecoregion that also serves as the main recharge zone for the High Plains (Ogallala) aquifer. Despite the national importance of this region in supplying irrigation water and supporting the US cattle industry, there have, until now, been no long-term, direct measurements of the components of the surface water balance. We have addressed this issue by installing energy balance/Bowen ratio (EBBR) flux towers in three key ecosystems. We report here the results of 7 years of measurements and interpret them in terms of annual weather variability. Additionally, these data have allowed us to make …

Chemical Modification Of Polysaccharides Using Reactive Extrusion, Pratik Bhandari

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The objective of this dissertation was to study the use of reactive extrusion for the chemical modifications of starch and cellulose. A carboxymethyl derivative of starch and carboxymethyl and acetate derivatives of cellulose were prepared using reactive extrusion.

Carboxymethyl starch with rapid swelling properties in water was prepared using reactive extrusion. This was achieved by controlling the gelatinization and through the use of NaOH by controlling the water/ethanol ratio. The effects of NaOH, H₂O, temperature, ethanol, sodium mono chloro acetate, sodium tripolyphosphate, citric acid, epichlorohydrin and extruder screw configuration on the degree of substitution of carboxymethyl starch were …

Methods For Enhancing Expression Of Secondary Cell Wall Cellulose Synthases In Plants, Chandrashekhar P. Joshi

Michigan Tech Patents

Described are methods for making transgenic plants capable of expressing secondary cell wall cellulose synthases and methods of enhancing expression of secondary cell wall cellulose synthases in plants. Also described are plants produced by the methods. Plants comprising at least three exogenous polynucleotides encoding secondary cell wall cellulose synthases are also provided.

Energy Issues Affecting Corn/Soybean Systems: Challenges For Sustainable Production, Douglas L. Karlen, David Archer, Adam Liska, Seth Meyer

Adam Liska Papers

Quantifying energy issues associated with agricultural systems, even for a two-crop corn (Zea mays L.) and soybean (Glycine max [L.] Merr.) rotation, is not a simple task. It becomes even more complicated if the goal is to include all aspects of sustainability (i.e., economic, environmental, and social). This Issue Paper examines energy issues associated with and affecting corn/soybean rotations by first defining the size of the system from both a U.S. and global perspective and then establishing boundaries based on the Farm Bill definition of sustainability. This structured approach is essential to help quantify energy issues within corn/soybean …

The Biological Systems Engineering Program In Nebraska 1895-2011, William Splinter

Department of Agricultural and Biological Systems Engineering: Faculty Publications

4. Preface: How Mechanization Has Impacted Food Production THE PIONEERS WHO ESTABLISHED THIS DEPARTMENT 8 Oscar Van Pelt Stout 10 Charles Russ Richards 12 J. Brownlee Davidson 14 Leon W. Chase FORMER FACULTY AND THEIR CONTRIBUTIONS 18 Laurence Froyd Seaton 20 Ivan D.Wood 22 Oscar W. Sjogren 23 Chauncey W. Smith 25 Elmer E. Brackett 27 Claud Kedzie Shedd 29 Ruby M. Loper 31 Lester F. Larsen 33 Lloyd W. Hurlbut 36 Rollin Schnieder 39 Howard D. Wittmuss 41 Deon Axthelm 46 Paul. E. Fischbach 48 Stuart O. Nelson 51 Kenneth von Bargen 56 John R. Davis 58 Robert W. …

Using Rapid Geomorphic Assessments To Assess Streambank Stability In Oklahoma Ozark Streams, Derek M. Heeren, Aaron R. Mittelstet, Garey A. Fox, Daniel E. Storm, Abdulsahib T. Al-Madhhachi, Taber L. Midgley, Ashley F. Stringer, Kevin B. Stunkel, Ron D. Tejral

Department of Agricultural and Biological Systems Engineering: Faculty Publications

High streambank erosion and failure rates on streams in the Ozark ecoregion of Oklahoma may be attributed to land use change and degradation of riparian areas. Numerous benefits may be achieved from streambank stabilization, but methods are needed to determine the most critical reaches for investing limited funds. Rapid geomorphic assessments (RGAs) have been used to aid in prioritizing stream reaches. This research (1) applied an existing RGA, the channel stability index (CSI), on several reaches along the Barren Fork Creek and Spavinaw Creek, and (2) modified the existing RGA to create an ecoregion-specific RGA called the Oklahoma Ozark streambank …

A 3-D Computational Fluid Dynamics Model For Forced Air Cooling Of Eggs Placed In Trays, Vikas Kumar, Ai Pheeng Wee, Sohan Birla, Jeyamkondan Subbiah, Harshanardhan Thippareddi

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Shell eggs must be cooled quickly after laying for controlling Salmonella Enteritidis (SE) growth. To fulfill a research need identified by Food Safety and Inspection Service (FSIS), a 3-D Computational Fluid Dynamics (CFD) model was developed to predict the temperature of eggs placed on a tray (6 rows × 5 columns) under forced air cooling. The continuity, momentum, and energy equations were solved along with standard k-ε turbulence model using PHOENICS software. The model was validated by conducting experiments in a wind tunnel at various air temperatures (7–11 °C) and velocities (0.3– 0.7 m/s). Root mean square error for predicting …

Climate Change Impacts On Streamflow, Water Quality, And Best Management Practices For The Shell And Logan Creek Watersheds In Nebraska, M. W. Van Liew, S. Feng, T. B. Pathak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Improvements in the management of water, sediment, and nutrients under future climatic conditions are needed to ensure increased crop and livestock production to meet greater global needs and the future availability of water for competing demands and protection against adverse water quality impairments. This study determined the impacts of future climate change scenarios on streamflow, water quality, and best management practices (BMPs) for two watersheds in Nebraska, USA. The Soil and Water Assessment Tool (SWAT) was employed to simulate streamflow, sediment, total nitrogen (N) and total phosphorus (P) from the Shell Creek Watershed near Columbus, Nebraska and the Logan Creek …

Global Estimation Of Evapotranspiration Using A Leaf Area Index-Based Surface Energy And Water Balance Model, H. Yan, S.Q. Wang, David P. Billesbach, Walter Oechel, J.H. Zhang, Tilden Meyers, Ta. Martin, Roser Matamala, Dennis D. Baldocchi, G. Bohrer, D. Dragoni, R. Scott

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Studies of global hydrologic cycles, carbon cycles and climate change are greatly facilitated when global estimates of evapotranspiration (E) are available. We have developed an air-relative-humidity-based two-source (ARTS) E model that simulates the surface energy balance, soil water balance, and environmental constraints on E. It uses remotely sensed leaf area index (L_ai) and surface meteorological data to estimate E by: 1) introducing a simple biophysical model for canopy conductance (G_c), defined as a constant maximum stomatal conductance g_smax of 12.2mm s⁻¹multiplied by air relative humidity (R_{h …}

Computational Fluid Dynamics In Congenital Heart Disease, William M. Decampli, I. Ricardo Argueta-Morales, Eduardo Divo, Alain J. Kassab

Mechanical Engineering - Daytona Beach

Computational fluid dynamics has been applied to the design, refinement, and assessment of surgical procedures and medical devices. This tool calculates flow patterns and pressure changes within a virtual model of the cardiovascular system. In the field of paediatric cardiac surgery, computational fluid dynamics is being used to elucidate the optimal approach to staged reconstruction of specific defects and study the haemodynamics of the resulting anatomical configurations after reconstructive or palliative surgery. In this paper, we review the techniques and principal findings of computational fluid dynamics studies as applied to a few representative forms of congenital heart disease.

A Thousand Tiny Pieces: The Federal Circuit’S Fractured Myriad Ruling, Lessons To Be Learned, And The Way Forward, Jonathan R. K. Stroud

IP Theory

No abstract provided.