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Quantitative Imaging Of Cell-Level Auxin Cytokinin Outputs In Soybean And Model Plants, Paul Gaillard 2020 South Dakota State University

Quantitative Imaging Of Cell-Level Auxin Cytokinin Outputs In Soybean And Model Plants, Paul Gaillard

Electronic Theses and Dissertations

Auxin and cytokinin are major plant hormones that orchestrate plant development. These hormones are involved in cell division and differentiation and their spatio‐temporal balance controls organ initiation and maturation. Even though independent transcriptional responses to these hormones have been characterized, their concomitant outputs have not been described. We developed methodology to determine auxin-cytokinin relative output (ACRO) ratios at cellular resolution. Hormone-specific fluorescent markers that localize to the nucleus were used to report transcriptional outputs of auxin and cytokinin. Multiphoton microscopy and image data processing were used to quantify their relative ratio at cellular level in three-dimensional tissue volumes. This strategy …


The Role Of Endophytes In Conferring Salinity Tolerance In Prairie Cordgrass And The Winter Wheat Mint Variety, Jeffery Bartel 2020 South Dakota State University

The Role Of Endophytes In Conferring Salinity Tolerance In Prairie Cordgrass And The Winter Wheat Mint Variety, Jeffery Bartel

Electronic Theses and Dissertations

Prairie Cordgrass (PCG) Spartina pectinata is a native grass to North America that survives saline conditions, can be harvested multiple times in a year, and can be used as biofuel. Endophytes are bacteria that reside in plant tissues and often have growthpromoting capabilities. They can assist plants by obtaining nutrients by improving the tolerance of plants against a broad range of stresses, such as plant pathogens, viruses, flooding, drought, acids, or bases. PCG can survive ocean levels of salinity. Endophytes that naturally bond with PCG may not only be able to not only help PCG but be transferred to other …


Soil Physical And Hydrological Properties, And Greenhouse Gas Emissions Under Integrated Crop-Livestock Agroecosystems, Navdeep Singh 2020 South Dakota State University

Soil Physical And Hydrological Properties, And Greenhouse Gas Emissions Under Integrated Crop-Livestock Agroecosystems, Navdeep Singh

Electronic Theses and Dissertations

Cover crops (CCs) and grazing play a critical role in successful implementation of the integrated crop-livestock system (ICLS) because they can have a direct impact on soils and greenhouse gas emissions. The objectives of this study were to (i) evaluate the short-term impacts of CCs [grass dominated cover crops (GdC) and legume dominated cover crops (LdC)] and grazed CCs and corn (Zea mays L.) residue under oat (Avena sativa L.)–CC–corn rotation on soil physical and hydrological properties; (ii) quantify the architecture of soil pores using X-ray computed tomography (CT) for soils managed under long-term ICLS, native grazed pasture and corn-soybean …


Cover Crop Nutrient Cycling On Western South Dakota Croplands, Justin Brown 2020 South Dakota State University

Cover Crop Nutrient Cycling On Western South Dakota Croplands, Justin Brown

Electronic Theses and Dissertations

Cover crops (CC) are widely gaining attention and implementation by producers. Much of this is due to the positive influence that CCs can have. Increases in crop yield, biological diversity, water infiltration, and nutrient cycling have been observed as well as reductions in wind and water erosion. The semiarid climate of western South Dakota presents a challenge in the use of CCs with the limited rainfall received. Little research has been done in this area on the effects of CCs on cropland. Three studies were performed to evaluate CCs on nutrient cycling, forage production and crop yield. First, is an …


Assembly Of Triticum Boeoticum Ssp. Aegilopoides And Triticum Monoccocum Genomes, Mustafa Aljadi 2020 South Dakota State University

Assembly Of Triticum Boeoticum Ssp. Aegilopoides And Triticum Monoccocum Genomes, Mustafa Aljadi

Electronic Theses and Dissertations

Wheat is ancient cereal. Wheat has n = 7 chromosomes which is belong to genus Triticum. The group Triticum monococcum sp. aegilopoides(boeoticum) is a diploid wheat (2n = 14). It has a morphology aspect of having narrow, flat spike which usually shatters before harvesting time. The domesticated type of T.boeoticum known as a diploid wheat of Triticum monococcum L. In this research we want to generate a high quality assembly of wild and domesticated type of Einkorn wheat. Our method is using Illumina short sequence reads assembled by both CLC, and W2RAP(Wheat/Whole- genome Robust Assembly Pipeline) software’s. In our approach …


Plant Microbial Interactions In Wheat: Fusarium Head Blight And Arbuscular Mycorrhizal Fungi, Yaqoob Rashid Thurston 2020 South Dakota State University

Plant Microbial Interactions In Wheat: Fusarium Head Blight And Arbuscular Mycorrhizal Fungi, Yaqoob Rashid Thurston

Electronic Theses and Dissertations

Plant microbial interactions consist of the many relationships between plants and microbes which involve studies that observe the biology and molecular genetics of pathological, symbiotic, and associative interactions. Worldwide studies involving these interactions are scarcely available in wheat (Triticum aestivum L.). In South Dakota (SD), wheat research is a major platform used to understand the nature and consequences of these interactions. Specifically, our research efforts here at South Dakota State University (SDSU) concentrate on two different, but valuable, interactions in wheat: the fungal pathogen that causes fusarium head blight (FHB) and the symbiotic interaction of arbuscular mycorrhizae fungi (AMF) with …


Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc 2020 South Dakota State University

Reverse Genetic Analysis Of Ehd1 For An Association Between Flowering Time And Seed Dormancy In Rice (Oryza Sativa L.), Yeter Karakoc

Electronic Theses and Dissertations

This research aimed to understand the evolutionary mechanism of seed dormancy and flowering time and to provide basic knowledge for the manipulation of germination features in crop breeding. Flowering time and seed dormancy are two adaptive traits for flowering plants. Seed dormancy (SD) helps regulate the timing of germination to promote the survival of seed-bearing plants in adverse environments. Cereal crops and weedy/wild relatives, such as Asian cultivated rice and weedy rice (Oryza sativa L.), have weak and strong seed dormancy, respectively. Thus, weedy and cultivated rice has been used as a model system to investigate mechanisms of seed dormancy …


Effect Of Management Decisions On Corn Yield Productivity And Stability In Environments With Contrasting Water Availability, Juan Ignacio Di Salvo 2020 University of Kentucky

Effect Of Management Decisions On Corn Yield Productivity And Stability In Environments With Contrasting Water Availability, Juan Ignacio Di Salvo

Theses and Dissertations--Plant and Soil Sciences

Corn (Zea Mays L.) is a grain crop with large productivity, but also elevated evapotranspiration demand, making it highly susceptible to periods of water stress occurring during critical reproductive stages. Environmental conditions in Kentucky make it possible to grow corn under rainfed conditions, but the crop is still likely to experience water stress during some times of the growing season depending on the year and location. There is limited information on the size of the yield gap due to water stress in Kentucky, and the timing and intensity of water deficit.

In addition, evaluating the interactive effects of hybrid …


Cover Crops And Cover Crop Mixes: Stratification Of Biological Effects, Landon M. Gibbs 2020 University of Kentucky

Cover Crops And Cover Crop Mixes: Stratification Of Biological Effects, Landon M. Gibbs

Theses and Dissertations--Plant and Soil Sciences

The potential nutrient cycling benefits from legumes (e.g. N2-fixation) and the high biomass potential of cereal rye are well known. Further studies are warranted to evaluate bi-culture mixtures and their effects on soil nutrient stratification and microbial enzyme activity because these two properties may be differently expressed (enhanced) by legume/grass mixes. The objectives of this study were: (1) show different cover crops and cover crop mixes containing grasses and legumes differentially stratify carbon and N; (2) show the change in microbial enzyme activity in soils planted with individual cover crops relative to cover crop mixes; 3) determine the …


Effect Of Urea Fertilizer Deep Placement Days After Transplanting Using Brri Prilled Urea Applicator On Transplanted Boro Rice Yield, Muhammad Rashed Al Mamun, K. Naher, Md Towfiqur Rahman, Md Anwar Hossen 2020 Sylhet Agricultural University

Effect Of Urea Fertilizer Deep Placement Days After Transplanting Using Brri Prilled Urea Applicator On Transplanted Boro Rice Yield, Muhammad Rashed Al Mamun, K. Naher, Md Towfiqur Rahman, Md Anwar Hossen

Biological Systems Engineering: Papers and Publications

Rice is the most important crop in the developing countries of Asia. In the south and south-east Asia, rain-fed and irrigated transplanted rice occupies nearly two-thirds of the rice-growing area and produces more than 80% of the rough rice. In these areas, prilled urea conventionally applied by farmers is very insufficient in the transplanted rice field, where severe losses occur (up to 60% of applied N) via NH3volatilization, denitrification, leaching, and runoff. Considering loss minimization, an experiment was conducted during the Boro season at Bangladesh Agricultural Development Corporation (BADC) in Sylhet to evaluate the performance of BRRI Prilled …


Cover Crop Productivity And Subsequent Soybean Yield In The Western Corn Belt, Katja Koehler-Cole, Roger W. Elmore, Humberto Blanco-Canqui, Charles A. Francis, Charles Shapiro, Christopher A. Proctor, Sabrina J. Ruis, Derek M. Heeren, Suat Irmak, Richard Ferguson 2020 University of Nebraska-Lincoln

Cover Crop Productivity And Subsequent Soybean Yield In The Western Corn Belt, Katja Koehler-Cole, Roger W. Elmore, Humberto Blanco-Canqui, Charles A. Francis, Charles Shapiro, Christopher A. Proctor, Sabrina J. Ruis, Derek M. Heeren, Suat Irmak, Richard Ferguson

Biological Systems Engineering: Papers and Publications

Cover crops (CC) in corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotations may prevent N loss and provide other ecosystem services but CC productivity in the western Corn Belt is limited by the short growing season. Our objective was to assess CC treatment and planting practice effects on CC biomass, spring soil nitrate concentrations, and soybean yield at two rainfed sites in eastern and one irrigated site in south-central Nebraska over 4 years. Cover crop treatments (cereal rye [Secale cereale L.] [RYE] and a mix of rye, legume, and brassica species [MIX]) were planted …


Vida Tank Mixtures For Postemergence Weed Control In Fallow, R. S. Currie, P. W. Geier 2020 Kansas State University

Vida Tank Mixtures For Postemergence Weed Control In Fallow, R. S. Currie, P. W. Geier

Kansas Agricultural Experiment Station Research Reports

The objective of the study was to compare Vida (pyraflufen ethyl) with various tank mix partners for weed control in fallow. All herbicides controlled flixweed and tansymustard 96% or more by 16 days after treatment. Treatments containing Spartan provided faster and better kochia control compared to other herbicides, and these treatments were the only ones to control kochia 95% or more late in the season.


The Agronomics Of Organically Managed Legume-Grass Mixtures For Ensiled Forage Production, Allen Wilder 2020 University of Vermont

The Agronomics Of Organically Managed Legume-Grass Mixtures For Ensiled Forage Production, Allen Wilder

Graduate College Dissertations and Theses

Legume-grass mixtures are commonly grown on livestock farms in northern New England that feed high forage diets. Such farms typically ferment stored feed into silage that can be fed throughout the year. Many legumes and grass species are available for use in temperate climates and information on the yield, forage quality, and fermentation characteristics of various legume-grass combinations would help farmers make informed decisions about species selection and optimum management for their forage programs.

One obstacle to evaluating numerous forage treatments in agronomic research is the high variability in silage dry matter content. We hypothesized that a method of artificial …


Long-Term Nitrogen, Phosphorus, And Potassium Fertilization Of Irrigated Grain Sorghum, A. Schlegel, H. D. Bond 2020 Kansas State University

Long-Term Nitrogen, Phosphorus, And Potassium Fertilization Of Irrigated Grain Sorghum, A. Schlegel, H. D. Bond

Kansas Agricultural Experiment Station Research Reports

Long-term research shows that phosphorus (P) and nitrogen (N) fertilizer must be applied to optimize production of irrigated grain sorghum in western Kansas. In 2019, N applied alone increased yields 66 bu/a, whereas N and P applied together increased yields up to 85 bu/a. Averaged across the past 10 years, N and P fertilization increased sorghum yields up to 78 bu/a. Application of 160 lb/a N (with P) produced the maxi­mum yield in 2019, which is slightly more than the 10-year average. Application of potassium (K) has had no effect on sorghum yield throughout the study period. Average grain N …


Effects Of Pre-Cutting Round Alfalfa Hay Bales On Forage Quality And Processing Time, W. E. Brown, E. Harms, J Heimsoth, J. McGinnis, C. I. Vahl, B. J. Bradford, M. J. Brouk 2020 Kansas State University

Effects Of Pre-Cutting Round Alfalfa Hay Bales On Forage Quality And Processing Time, W. E. Brown, E. Harms, J Heimsoth, J. Mcginnis, C. I. Vahl, B. J. Bradford, M. J. Brouk

Kansas Agricultural Experiment Station Research Reports

Round hay balers with knives that cut the hay as it enters the baling chamber reduce the particle size upon baling, and eliminate the need for a tub grinder. The objective of this study was to evaluate the effects of a round hay baler with knives on forage quality of alfalfa hay at baling and after storage, and the effects of the processing method on nutrient composition and particle size distribution. Alfalfa hay was baled (560 M Megawide HC2, John Deere, Moline, IL) with knives every 4 inches (CUT; theo­retical length of cut) or without knives (NORM). At baling and …


Association Of Horizontal Silo Pad Type, Elevation And Core Depth With Indicators Of Silo Ramp Hygiene, Forage Quality, And Digestibility, W. E. Brown, N. M. Bello, M. J. Brouk 2020 Kansas State University

Association Of Horizontal Silo Pad Type, Elevation And Core Depth With Indicators Of Silo Ramp Hygiene, Forage Quality, And Digestibility, W. E. Brown, N. M. Bello, M. J. Brouk

Kansas Agricultural Experiment Station Research Reports

Horizontal silo piles without walls are constructed using packing equipment to adequately pack the forage for air exclusion. During packing, the equipment uses a ramp of forage to access the top of the pile, potentially introducing soil into the forage when the base of the silo is made of soil. Soil contains microorganisms which may cause malfermentation and pose health risks to livestock. The objective of this study was to assess the association of horizontal silo pad type, elevation, and core depth with indicators related to silage hygiene and nutrient quality. We hypothesized that ash and mineral content, microbiological profile, …


Weather Information For Garden City, 2019, J. Elliott 2020 Kansas State University

Weather Information For Garden City, 2019, J. Elliott

Kansas Agricultural Experiment Station Research Reports

Summary of 2019 weather for research conducted at the Garden City field location.


Weather Information For Tribune, H. D. Bond, J. Slattery 2020 Kansas State University

Weather Information For Tribune, H. D. Bond, J. Slattery

Kansas Agricultural Experiment Station Research Reports

Summary of 2019 weather for research conducted at the Tribune field location.


Wheat And Grain Sorghum In Four-Year Rotations, A. Schlegel, J. Holman, A. Burnett 2020 Kansas State University

Wheat And Grain Sorghum In Four-Year Rotations, A. Schlegel, J. Holman, A. Burnett

Kansas Agricultural Experiment Station Research Reports

In 1996, an effort began to quantify soil water storage, crop water use, and crop pro­ductivity on dryland systems in western Kansas. Research on 4-year crop rotations with wheat and grain sorghum was initiated at the Southwest Research-Extension Center near Tribune, KS. Rotations were wheat-wheat-sorghum-fallow (WWSF), wheat-sorghum-sorghum-fallow (WSSF), and continuous wheat (WW). Soil water at wheat planting averaged about 9 in. following sorghum, which is about 3 in. more than the average for the second wheat crop in a WWSF rotation. Soil water at sorghum planting was only about 1.5 in. less for the second sorghum crop compared to sorghum …


Large-Scale Dryland Cropping Systems, A. Schlegel, L. Haag, A. Burnett 2020 Kansas State University

Large-Scale Dryland Cropping Systems, A. Schlegel, L. Haag, A. Burnett

Kansas Agricultural Experiment Station Research Reports

This study was conducted from 2008–2019 at the Kansas State University Southwest Research- Extension Center near Tribune, KS. The purpose of the study was to identify whether more intensive cropping systems can enhance and stabilize production in rainfed cropping systems to optimize economic crop production, more efficiently capture and utilize scarce precipitation, and maintain or enhance soil resources and environmental quality. The crop rotations evaluated were continuous grain sorghum (SS), wheat-fallow (WF), wheat-corn-fallow (WCF), wheat-sorghum-fallow (WSF), wheat-corn-sorghum-fallow (WCSF), and wheat-sorghum-corn-fallow (WSCF). All rotations were grown using no-tillage practices except for WF, which was grown using reduced-tillage. The efficiency of precipitation …


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