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Perches As Cooling Devices For Reducing Heat Stress In Caged Laying Hens: A Review, Jiaying Hu, Yijie Xiong 2021 Purdue University,

Perches As Cooling Devices For Reducing Heat Stress In Caged Laying Hens: A Review, Jiaying Hu, Yijie Xiong

Department of Animal Science: Faculty Publications

Heat stress is one of the most detrimental environmental challenges affecting the biological process and the related production performance of farm animals, especially in poultry. Commercial laying hens have been bred (selected) for high egg production, resulting in increased sensitivity to heat stress due to breeding-linked metabolic heat production. In addition, laying hens are prone to heat stress due to their inadequate species-specific cooling mechanisms resulting in low heat tolerance. In addition, hens have no sweat glands and feathering covers almost their entire body to minimize body heat loss. The poultry industry and scientists are developing cooling methods to prevent …


Micrornas As Biomarkers For Early Diagnosis, Prognosis, And Therapeutic Targeting Of Ovarian Cancer, Yegane Mirahmadi, Reza Nabavi, Fourough Taheri, Mohammad Mahdi Samadian, Zari Naderi Ghale-Noie, Mahsa Farjami, Abbas Samadi-khouzani, Meysam Yousefi, Sara Azhdari, Arash Salmaninejad, Amirhossein Sahebkar 2021 Mashhad University of Medical Sciences,

Micrornas As Biomarkers For Early Diagnosis, Prognosis, And Therapeutic Targeting Of Ovarian Cancer, Yegane Mirahmadi, Reza Nabavi, Fourough Taheri, Mohammad Mahdi Samadian, Zari Naderi Ghale-Noie, Mahsa Farjami, Abbas Samadi-Khouzani, Meysam Yousefi, Sara Azhdari, Arash Salmaninejad, Amirhossein Sahebkar

Department of Animal Science: Faculty Publications

Ovarian cancer is the major cause of gynecologic cancer-related mortality. Regardless of outstanding advances, which have been made for improving the prognosis, diagnosis, and treatment of ovarian cancer, the majority of the patients will die of the disease. Late-stage diagnosis and the occurrence of recurrent cancer after treatment are the most important causes of the high mortality rate observed in ovarian cancer patients. Unraveling the molecular mechanisms involved in the pathogenesis of ovarian cancer may help find new biomarkers and therapeutic targets for ovarian cancer. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression, mostly at the posttranscriptional stage, …


Profile Of Producers And Production Of Dry-Aged Beef In Brazil, Jonatã Henrique Rezende-de-Souza, Flavio Andre Bolini Cardello, Ana Paula Moraes de Paula, Felipe A. Ribeiro, Chase C. Calkins, Sérgio Bertelli Pflanzer 2021 State University of Campinas, Brazil

Profile Of Producers And Production Of Dry-Aged Beef In Brazil, Jonatã Henrique Rezende-De-Souza, Flavio Andre Bolini Cardello, Ana Paula Moraes De Paula, Felipe A. Ribeiro, Chase C. Calkins, Sérgio Bertelli Pflanzer

Department of Animal Science: Faculty Publications

No information is currently available on the profile of producers and production process of dry-aged beef in Brazil, to the best of the authors’ knowledge. We surveyed 37 Brazilian companies that were producing dry-aged beef in 2020 to investigate this market. The absolute and relative frequency of responses was calculated to obtain the sum, average, minimum, and maximum values. From the respondents, dry-aged beef was first produced in 2009, and most producers are located in big cities. Most respondents control and monitor chamber temperature; however, humidity and air velocity only are monitored. The aging period (mostly between 22 to 60 …


Predicting Carcass Cut Yields In Cattle From Digital Images Using Artificial Intelligence, Darragh Matthews, Thierry Pabiou, Ross D. Evans, Christian Beder, Aengus Daly 2021 Department of Mathematics, Munster Technological University, Cork , Ireland; Irish Breeding Federation, Bandon, Co. Cork, Ireland

Predicting Carcass Cut Yields In Cattle From Digital Images Using Artificial Intelligence, Darragh Matthews, Thierry Pabiou, Ross D. Evans, Christian Beder, Aengus Daly

Department of Mathematics Publications

Deep Learning (DL) has proven to be a successful tool for many image classification problems but has yet to be applied to carcass images. The aim of this study was to train DL models to predict carcass cut yields and compare predictions to more standard machine learning (ML) methods. Three approaches were undertaken to predict the grouped carcass cut yields of Grilling cuts and Roasting cuts from a large dataset of 54,598 and 69,246 animals respectively. The approaches taken were (1) animal phenotypic data used as features for a range of ML algorithms, (2) carcass images used to train Convolutional …


Impact Of Feeding Syngenta Enogen® Feed Corn Compared To Control Corn In Different Diet Scenarios To Finishing Beef Cattle, Stacia M. Volk, Hannah H. Wilson, Kathryn J. Hanford, James C. MacDonald, Galen E. Erickson 2021 University of Nebraska, Lincoln

Impact Of Feeding Syngenta Enogen® Feed Corn Compared To Control Corn In Different Diet Scenarios To Finishing Beef Cattle, Stacia M. Volk, Hannah H. Wilson, Kathryn J. Hanford, James C. Macdonald, Galen E. Erickson

Department of Animal Science: Faculty Publications

The objective of this pooled statistical analysis was to evaluate Syngenta Enogen® Feed Corn (EFC) versus conventional corn (CON) when fed as either dry-rolled corn (DRC) or highmoisture corn (HMC) for effects on finishing beef cattle performance and carcass characteristics. Corns were evaluated in diets with byproduct inclusion rates of 0, 15, 18, 20, and 30% distiller grains or 25 and 35% Sweet Bran® (a commercial corn gluten feed product). Seven trials (n = 1856) consisting of 200 pen means comparing 26 diet treatments were analyzed using regression in a pooled analysis. When EFC was processed as DRC, the gain …


Localization Of Kisspeptin, Nkb, And Nk3r In The Hypothalamus Of Gilts Treated With The Progestin Altrenogest, Ashley N. Lindo, Jennifer F. Thorson, Michelle N. Bedenbaugh, Richard B. McCosh, Justin A. Lopez, Samantha A. Young, Lanny J. Meadows, Elizabeth C. Bowdridge, Chrysanthi Fergani, Bradley A. Freking, Michael N. Lehman, Stanley M. Hileman, Clay A. Lents 2021 West Virginia University

Localization Of Kisspeptin, Nkb, And Nk3r In The Hypothalamus Of Gilts Treated With The Progestin Altrenogest, Ashley N. Lindo, Jennifer F. Thorson, Michelle N. Bedenbaugh, Richard B. Mccosh, Justin A. Lopez, Samantha A. Young, Lanny J. Meadows, Elizabeth C. Bowdridge, Chrysanthi Fergani, Bradley A. Freking, Michael N. Lehman, Stanley M. Hileman, Clay A. Lents

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Mechanisms in the brain controlling secretion of gonadotropin hormones in pigs, particularly luteinizing hormone (LH), are poorly understood. Kisspeptin is a potent LH stimulant that is essential for fertility in many species, including pigs. Neurokinin B (NKB) acting through neurokinin 3 receptor (NK3R) is involved in kisspeptin-stimulated LH release, but organization of NKB and NK3R within the porcine hypothalamus is unknown. Hypothalamic tissue from ovariectomized (OVX) gilts was used to determine the distribution of immunoreactive kisspeptin, NKB, and NK3R cells in the arcuate nucleus (ARC). Almost all kisspeptin neurons coexpressed NKB in the porcine ARC. Immunostaining for NK3R was distributed …


Non - Naturally Occurring Porcine Reproductive And Respiratory Syndrome Virus ( Prrsv ) And Methods Of Using, Hiep Lai Xuan Vu, Fernando Osorio, William W. Laegreid, Asit K. Pattnaik, Fangrui Ma 2021 Lincoln , NE

Non - Naturally Occurring Porcine Reproductive And Respiratory Syndrome Virus ( Prrsv ) And Methods Of Using, Hiep Lai Xuan Vu, Fernando Osorio, William W. Laegreid, Asit K. Pattnaik, Fangrui Ma

Department of Animal Science: Faculty Publications

A non - naturally occurring porcine reproductive and respi ratory syndrome virus ( PRRSV ) is provided herein , and methods of making and using the non - naturally occurring PRRSV also are provided


Relationship Of Molecular Breeding Value For Beef Tenderness With Heifer Traits Through Weaning Of Their First Calf, Robert A. Cushman, Gary L. Bennett, Richard G. Tait, Anthony K. McNeel, Eduardo Casas, Timothy P.L. Smith, Harvey C. Freetly 2021 Roman L. Hruska US Meat Animal Research Center

Relationship Of Molecular Breeding Value For Beef Tenderness With Heifer Traits Through Weaning Of Their First Calf, Robert A. Cushman, Gary L. Bennett, Richard G. Tait, Anthony K. Mcneel, Eduardo Casas, Timothy P.L. Smith, Harvey C. Freetly

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Polymorphisms in μ-calpain (CAPN1) that beneficially associate with beef tenderness are reported to antagonistically associate with calving day in beef heifers and post-partum interval to estrus in beef cows. We, therefore, hypothesized that a molecular breeding value for slice shear force, calculated based on CAPN1 and calpastatin (CAST) genotypes, would demonstrate an antagonistic relationship between genomically predicted slice shear force and ordinal calving date in replacement beef heifers. A secondary objective of this study was to evaluate the association of a polymorphism in diacylglycerol O-acyltransferase (DGAT1) with reproductive traits in beef heifers. One hundred eighty-seven MARC III heifers (¼ Angus, …


Validation Of High-Resolution Melting Assays For The Detection Of Virulent Strains Of Escherichia Coli O26 And O111 In Beef And Pork Enrichment Broths, Frank J. Velez, Joseph M. Bosilevac, Prashant Singh 2021 Florida State University

Validation Of High-Resolution Melting Assays For The Detection Of Virulent Strains Of Escherichia Coli O26 And O111 In Beef And Pork Enrichment Broths, Frank J. Velez, Joseph M. Bosilevac, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Extensive validation of diagnostic assays using widely collected surveillance samples is critical for developing pathogen detection assays. The detection of potentially virulent E. coli strains is critical to the red meat industry. We previously developed two high-resolution melting (HRM) assays for detecting potentially virulent and avirulent E. coli O26 and O111 strains. Assays were validated using enriched beef (n = 36) and pork (n = 36) samples collected as part of a U.S. federal regulatory surveillance program. Data from this study showed more than 90% sensitivity and specificity for both the HRM assays, demonstrating suitability for the red meat industry …


Genomic Analysis Of Shiga Toxin-Containing Escherichia Coli O157:H7 Isolated From Argentinean Cattle, Ariel Amadio, James L. Bono, Matías Irazoqui, Mariano Larzábal, Wanderson Marques da Silva, María Florencia Eberhardt, Nahuel A. Riviere, David Gally, Shannon D. Manning, Angel Cataldi 2021 Instituto Nacional de Tecnologia Agropecuaria Buenos Aires

Genomic Analysis Of Shiga Toxin-Containing Escherichia Coli O157:H7 Isolated From Argentinean Cattle, Ariel Amadio, James L. Bono, Matías Irazoqui, Mariano Larzábal, Wanderson Marques Da Silva, María Florencia Eberhardt, Nahuel A. Riviere, David Gally, Shannon D. Manning, Angel Cataldi

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina has the highest pediatric HUS incidence worldwide with 12–14 cases per 100,000 children. Herein, we assessed the genomes of EHEC O157:H7 isolates recovered from cattle in the humid Pampas of Argentina. According to phylogenetic studies, EHEC O157 can be divided into clades. Clade 8 strains that were classified as hypervirulent. Most of the strains of this clade have a Shiga toxin stx2a-stx2c genotype. To better understand the molecular bases …


Whole-Genome Sequencing To Investigate A Possible Genetic Basis Of Perosomus Elumbis In A Calf Resulting From A Consanguineous Mating, Alexa M. Barber, Alyssa Helms, Riley Thompson, Brian K. Whitlock, David J. Steffen, Jessica Lynn Petersen 2021 University of Nebraska-Lincoln

Whole-Genome Sequencing To Investigate A Possible Genetic Basis Of Perosomus Elumbis In A Calf Resulting From A Consanguineous Mating, Alexa M. Barber, Alyssa Helms, Riley Thompson, Brian K. Whitlock, David J. Steffen, Jessica Lynn Petersen

Department of Animal Science: Faculty Publications

Perosomus elumbis (PE) is a lethal, congenital defect marked by aplasia of the lumbar and sacral spine and spinal cord. Contracture of the hind limbs is also commonly observed in affected individuals. PE has been reported in many domestic species, with numerous case reports in Holstein cattle in the past two decades (Jones, 1999; Karakaya et al., 2013; Agerholm et al., 2014). The etiology of PE remains unknown. In one instance, a stillborn Holstein calf with PE was found to be infected with Bovine Viral Diarrhea Virus (BVDV) (Karakaya et al., 2013), and thus, it is possible that PE may …


Using Beet Pulp To Adapt Cattle To Finishing Diets Compared To Traditional Grain Adaptation With Alfalfa Hay, C. J. Schneider, Matt K. Luebbe, K. H. Jenkins, Stephanie A. Furman Furman, Galen E. Erickson, Terry J. Klopfenstein 2021 University of Nebraska-Lincoln

Using Beet Pulp To Adapt Cattle To Finishing Diets Compared To Traditional Grain Adaptation With Alfalfa Hay, C. J. Schneider, Matt K. Luebbe, K. H. Jenkins, Stephanie A. Furman Furman, Galen E. Erickson, Terry J. Klopfenstein

Department of Animal Science: Faculty Publications

A study was conducted to compare grain adaptation programs using beet pulp (BP) to traditional grain adaptation with alfalfa hay (AH). Yearling crossbred steers (n = 232; BW = 326 ± 14.5 kg) were separated into 3 weight blocks, stratified by BW, and assigned randomly, within strata, to 18 feedlot pens, with 12 or 13 steers per pen. Treatments were imposed during grain adaptation (21 d) using 3 grain adaptation programs. Within each grain adaptation program, 4 step rations were fed for 3, 4, 7, and 7 d. Each program increased dry-rolled corn inclusion while roughage inclusion decreased. In the …


Characterisation Of Early Positive Mcr‐1 Resistance Gene And Plasmidome In Escherichia Coli Pathogenic Strains Associated With Variable Phylogroups Under Colistin Selection, Guerrino Macori, Scott V. Nguyen, Ankita Naithani, Daniel Hurley, Li Bai, Farid El Garch, Frédérique Woehrlé, Christine Miossec, Benjamin Roques, Peadar O’gaora, James L. Bono, Séamus Fanning 2021 University College Dublin

Characterisation Of Early Positive Mcr‐1 Resistance Gene And Plasmidome In Escherichia Coli Pathogenic Strains Associated With Variable Phylogroups Under Colistin Selection, Guerrino Macori, Scott V. Nguyen, Ankita Naithani, Daniel Hurley, Li Bai, Farid El Garch, Frédérique Woehrlé, Christine Miossec, Benjamin Roques, Peadar O’Gaora, James L. Bono, Séamus Fanning

Roman L. Hruska U.S. Meat Animal Research Center: Reports

An antibiotic susceptibility monitoring programme was conducted from 2004 to 2010, re-sulting in a collection of 143 Escherichia coli cultured from bovine faecal samples (diarrhoea) and milk‐aliquots (mastitis). The isolates were subjected to whole‐genome sequencing and were distrib-uted in phylogroups A, B1, B2, C, D, E, and G with no correlation for particular genotypes with pathotypes. In fact, the population structure showed that the strains belonging to the different phy-logroups matched broadly to ST complexes; however, the isolates are randomly associated with the diseases, highlighting the necessity to investigate the virulence factors more accurately in order to identify the mechanisms …


Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang 2021 Melbourne University

Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat tenderness is an important quality trait critical to consumer acceptance, and determines satisfaction, repeat purchase and willingness-to-pay premium prices. Recent advances in tenderness research from a variety of perspectives are presented. Our understanding of molecular factors influencing tenderization are discussed in relation to glycolysis, calcium release, protease activation, apoptosis and heat shock proteins, the use of proteomic analysis for monitoring changes, proteomic biomarkers and oxidative/nitrosative stress. Each of these structural, metabolic and molecular determinants of meat tenderness are then discussed in greater detail in relation to animal variation, postmortem influences, and changes during cooking, with a focus on recent …


Effect Of A Trace Mineral Injection On Performance And Trace Mineral Status Of Beef Cows And Calves, Carmen J. Willmore, John B. Hall, Mary E. Drewnoski 2021 University of Idaho

Effect Of A Trace Mineral Injection On Performance And Trace Mineral Status Of Beef Cows And Calves, Carmen J. Willmore, John B. Hall, Mary E. Drewnoski

Department of Animal Science: Faculty Publications

The objective was to determine the effects of an injectable trace mineral (TMI; Multimin 90) containing copper (Cu), manganese (Mn), selenium (Se), and zinc (Zn) on trace mineral status and the resulting impacts on reproduction of beef cows and the growth of their calves. Beef cows (n = 200) were assigned to receive TMI or no injection (CON) prior to calving and breeding over two consecutive years. Calves born to cows receiving TMI also received TMI at birth in both years and at 49 ± 1.3 days of age in year 1. The TMI increased (p = 0.01) liver Zn …


Classifying Ingestive Behavior Of Dairy Cows Via Automatic Sound Recognition, Guoming Li, Yijie Xiong, Qian Du, Zhengxiang Shi, Richard S. Gates 2021 Iowa State University

Classifying Ingestive Behavior Of Dairy Cows Via Automatic Sound Recognition, Guoming Li, Yijie Xiong, Qian Du, Zhengxiang Shi, Richard S. Gates

Department of Animal Science: Faculty Publications

Determining ingestive behaviors of dairy cows is critical to evaluate their productivity and health status. The objectives of this research were to (1) develop the relationship between forage species/heights and sound characteristics of three different ingestive behaviors (bites, chews, and chew-bites); (2) comparatively evaluate three deep learning models and optimization strategies for classifying the three behaviors; and (3) examine the ability of deep learning modeling for classifying the three ingestive behaviors under various forage characteristics. The results show that the amplitude and duration of the bite, chew, and chew-bite sounds were mostly larger for tall forages (tall fescue and alfalfa) …


Pilot-Scale H2S And Swine Odor Removal System Using Commercially Available Biochar, Kyoung S. Ro, Brian Woodbury, Mindy Spiehs, Ariel A. Szogi, Philip J. Silva, Okhwa Hwang, Sungback Cho 2021 United States Department of Agriculture

Pilot-Scale H2S And Swine Odor Removal System Using Commercially Available Biochar, Kyoung S. Ro, Brian Woodbury, Mindy Spiehs, Ariel A. Szogi, Philip J. Silva, Okhwa Hwang, Sungback Cho

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Although biochars made in laboratory seem to remove H2S and odorous compounds effectively, very few studies are available for commercial biochars. This study evaluated the efficacy of a commercial biochar (CBC) for removing H2S and odorous volatile organic compounds (VOCs). We found that the well-known Ergun equation may not be adequate in predicting pressure drop for properly sizing a ventilation system. The H2S breakthrough adsorption capacity of the CBC was 2.51 mg/g under humid conditions, which was much higher than that for dry conditions. The breakthrough capacity increased with the influent concentration of H2S. The efficacy of a pilot-scale biochar …


The Ovarian Follicle Of Ruminants: The Path From Conceptus To Adult, Jennifer L. Juengel, Robert A. Cushman, Joëlle Dupont, Stéphane Fabre, Richard G. Lea, Graeme B. Martin, Francesca Mossa, Janet L. Pitman, Christopher A. Price, Peter Smith 2021 AgResearch Invermay Agricultural Centre

The Ovarian Follicle Of Ruminants: The Path From Conceptus To Adult, Jennifer L. Juengel, Robert A. Cushman, Joëlle Dupont, Stéphane Fabre, Richard G. Lea, Graeme B. Martin, Francesca Mossa, Janet L. Pitman, Christopher A. Price, Peter Smith

Roman L. Hruska U.S. Meat Animal Research Center: Reports

This review resulted from an international workshop and presents a consensus view of critical advances over the past decade in our understanding of follicle function in ruminants. The major concepts covered include: (1) the value of major genes; (2) the dynamics of fetal ovarian development and its sensitivity to nutritional and environmental influences; (3) the concept of an ovarian follicle reserve, aligned with the rise of anti-Müllerian hormone as a controller of ovarian processes; (4) renewed recognition of the diverse and important roles of theca cells; (5) the importance of follicular fluid as a microenvironment that determines oocyte quality; (6) …


Evolution Of The Sheep Industry And Genetic Research In The United States: Opportunities For Convergence In The Twenty-First Century, J. W. Thorne, B. M. Murdoch, B. A. Freking, R. R. Redden, Thomas W. Murphy, J. B. Taylor, Harvey D. Blackburn 2021 Texas A&M AgriLife

Evolution Of The Sheep Industry And Genetic Research In The United States: Opportunities For Convergence In The Twenty-First Century, J. W. Thorne, B. M. Murdoch, B. A. Freking, R. R. Redden, Thomas W. Murphy, J. B. Taylor, Harvey D. Blackburn

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The continuous development and application of technology for genetic improvement is a key element for advancing sheep production in the United States. The US sheep industry has contracted over time but appears to be at a juncture where a greater utilization of technology can facilitate industry expansion to new markets and address inefficiencies in traditional production practices. Significant transformations include the increased value of lamb in relation to wool, and a downtrend in large-scale operations but a simultaneous rise in small flocks. Additionally, popularity of hair breeds not requiring shearing has surged, particularly in semi-arid and subtropical US environments. A …


Breed And Heterotic Effects For Mature Weight In Beef Cattle, Madeline J. Zimmermann, Larry A. Kuehn, Matthew L. Spangler, R. M. Thallman, Warren M. Snelling, Ronald M. Lewis 2021 University of Nebraska-Lincoln

Breed And Heterotic Effects For Mature Weight In Beef Cattle, Madeline J. Zimmermann, Larry A. Kuehn, Matthew L. Spangler, R. M. Thallman, Warren M. Snelling, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Cow mature weight (MWT) is heritable and affects the costs and efficiency of a breeding operation. Cow weight is also influenced by the environment, and the relationship between the size and profitability of a cow varies depending on production system. Producers, therefore, need tools to incorporate MWT in their selection of cattle breeds and herd replacements. The objective of this study was to estimate breed and heterotic effects for MWT using weight-age data on crossbred cows. Cow's MWT at 6 yr was predicted from the estimated parameter values-asymptotic weight and maturation constant (k)-from the fit of the Brody function to …


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