Synthesis And Development Of Zwitterionic Pei (Zpei) For Optimized Delivery Of Nucleic Acids,
2017
University of Kentucky
Synthesis And Development Of Zwitterionic Pei (Zpei) For Optimized Delivery Of Nucleic Acids, Joseph Raleigh Duke Iii
Theses and Dissertations--Chemistry
Gene therapy holds promise for the treatment a wide range of diseases ranging from cystic fibrosis to cardiovascular disease to cancer. The need for safe and efficient gene delivery methods remains the primary barrier to human gene therapy. Non-viral vector materials, including polymers, can be designed to be biocompatible and non-immunogenic, but lack the efficiency to be clinically relevant. Gene therapy awaits the development of new materials that are both safe and efficient. Here, we have synthesized a series of modified zwitterionic polymers based on the common transfecting agent polyethylenimine (PEI). Using a variety of biochemical and biophysical methods we …
พอลิแล็กทิกแอซิด/พอลิไวนิลแอลกอฮอล์/แกรฟีนนาโนคอมพอสิต,
2017
คณะวิทยาศาสตร์
พอลิแล็กทิกแอซิด/พอลิไวนิลแอลกอฮอล์/แกรฟีนนาโนคอมพอสิต, จาฬุพักต์ รัตนคช
Chulalongkorn University Theses and Dissertations (Chula ETD)
งานวิจัยนี้มีวัตถุประสงค์เพื่อพัฒนาวิธีการเตรียมพอลิแล็กทิกแอซิด/พอลิไวนิลแอลกอฮอล์/แกรฟีนนาโนคอมพอสิต รวมถึงศึกษาอิทธิพลของการเติมอนุภาคแกรฟีนต่อสัณฐานวิทยา สมบัติเชิงกล สมบัติทางความร้อน และสมบัติการนำไฟฟ้าของวัสดุนาโนคอมพอสิตที่เตรียมได้ โดยเริ่มด้วยการสังเคราะห์แกรฟีนจากแกรไฟต์ด้วยวิธีของฮัมเมอร์ และใช้กรดแอลแอสคอร์บิกเป็นตัวรีดิวซ์ พิสูจน์เอกลักษณ์ของอนุภาคแกรฟีนที่เตรียมได้ด้วยเทคนิคอินฟราเรดสเปกโทรสโกปีและเทคนิคเอกซเรย์ดิฟแฟรกชัน จากนั้นเตรียมของผสมระหว่างพอลิไวนิลแอลกอฮอล์กับแกรฟีนที่ปริมาณต่าง ๆ ด้วยวิธีแช่เยือกแข็ง แล้วนำไปผสมกับพอลิแล็กทิกแอซิดด้วยเครื่องอัดรีดสกรูคู่และขึ้นรูปเป็นชิ้นงานด้วยกระบวนการอัดแบบ ศึกษาสัณฐานวิทยาเพื่อวิเคราะห์อันตรกิริยาของผสมระหว่างพอลิไวนิลแอลกอฮอล์กับแกรฟีนด้วยกล้องจุลทรรศน์แบบคอนโฟคอล และวิเคราะห์ภาคตัดขวางของชิ้นงานคอมพอสิตด้วยกล้องจุลทรรศน์อิเล็กตรอนแบบส่องกราด ผลการทดสอบสมบัติเชิงกล สมบัติทางความร้อน และสมบัติการนำไฟฟ้าของวัสดุนาโนคอมพอสิตที่เตรียมได้ พบว่าปริมาณผลึกลดลงเมื่อปริมาณแกรฟีนเพิ่มขึ้น แต่ไม่ส่งผลต่ออุณหภูมิกลาสทรานสิชัน อุณหภูมิหลอมเหลวผลึก และอุณหภูมิการเกิดผลึก อีกทั้งการเติมแกรฟีนที่มีการใช้พอลิไวนิลแอลกอฮอล์เป็นตัวพาลงในพอลิเมอร์เมทริกซ์ ทำให้ความทนแรงดึง และความยืดสูงสุด ณ จุดขาด มีแนวโน้มลดลงด้วย อย่างไรก็ดีการเติมแกรฟีนในปริมาณน้อย ๆ สามารถช่วยเพิ่มความทนแรงดึงได้เมื่อเปรียบเทียบกับงานวิจัยก่อนหน้าที่ไม่มีการเติมตัวพาหรือตัวช่วยเพิ่มการกระจายตัว นอกจากนี้พบว่าปริมาณแกรฟีนที่เพิ่มขึ้น ทำให้ค่าการนำไฟฟ้าของคอมพอสิตเมื่อเทียบกับพอลิแล็กทิกแอซิดบริสุทธิ์เพิ่มขึ้น คอมพอสิตที่พัฒนาขึ้นสามารถนำไปประยุกต์ในงานที่ต้องการสมบัติการต้านทานไฟฟ้าสถิตย์ได้
3d Printing Of Graphene/Thermoplastic Elastomer Composites,
2017
Faculty of Sciences
3d Printing Of Graphene/Thermoplastic Elastomer Composites, Warrayut Kanabenja
Chulalongkorn University Theses and Dissertations (Chula ETD)
Graphene based thermoplastic polyurethane composites filaments was prepared by melt blending process prior to fabricate with 3D printing technique. The effects of different graphene loading (0.05 wt% - 0.20 wt%) in TPU matrix on the properties of composites were studied. Initially, graphite was successfully oxidized to graphite oxide following Hummer’s method and the reduction of graphene oxide to graphene by L-ascorbic acid was also achieved as being confirmed by the FTIR and XRD results. TPU and TPU/graphene nanocomposites were investigated for their thermal, mechanical, physical and electrical properties. Thermal stability of TPU/graphene composites was improved against neat TPU. The first …
Metal-Salen Derivatives For Electro-Reduction Of Carbon Dioxide,
2017
Faculty of Science
Metal-Salen Derivatives For Electro-Reduction Of Carbon Dioxide, Kantima Chitchak
Chulalongkorn University Theses and Dissertations (Chula ETD)
Copper(II) salen, salophen-based ligands, and their corresponding copper complexes were prepared and utilized for the electrocatalytic reduction of carbon dioxide. Controlled-potential electrolysis of copper(II) salen electrocatalyst with carbon dioxide in non-aqueous solution was investigated to obtain the optimized electrolysis condition including the applied potential and proton donor quantity. With the applied potential of –2.10 V, carbon monoxide (3.50-16.30%) was obtained as the only product in gas phase. Methane was additionally found with the addition of proton donor and its maximum percentage (0.39 ± 0.00%) were obtained from the electrolysis with 25.00 mM proton donor and 2.00 mM copper(II) salen. Moreover, …
Synthesis Of Ni-Tin Incorporated Porous Silica Catalysts For Dry Reforming Of Methane,
2017
Faculty of Science
Synthesis Of Ni-Tin Incorporated Porous Silica Catalysts For Dry Reforming Of Methane, Maslin Chotirach
Chulalongkorn University Theses and Dissertations (Chula ETD)
A new titanium nitride (TiN) promoted nickel catalyst was synthesized and tested as an alternative catalyst in the dry reforming of methane (DRM). The series of this catalyst with various Ni and TiN contents was prepared in two steps, direct synthesis of SBA-15 in the presence of TiN, followed by the impregnation of Ni. The influence of TiN promoted Ni-based catalyst on dry reforming reaction was investigated at the temperature of 700ºC and atmospheric pressure for a duration of 4 h, using feed ratio of CH4/CO2 = 1. The promising catalysts, that gave highest CO2 and CH4 conversions as well …
Electrochemical Properties Of Lnsr3-Xcaxfe3-Ybyo10 (Ln = La, Pr And Sm; X = 0-1.0; B = Co, Ni And Cu; Y = 0-1.5) For Intermediate Temperature Solid Oxide Fuel Cells,
2017
Faculty of Science
Electrochemical Properties Of Lnsr3-Xcaxfe3-Ybyo10 (Ln = La, Pr And Sm; X = 0-1.0; B = Co, Ni And Cu; Y = 0-1.5) For Intermediate Temperature Solid Oxide Fuel Cells, Nicharee Wongsawatgul
Chulalongkorn University Theses and Dissertations (Chula ETD)
To enhance the electrochemical performance of cathode material for intermediate temperature solid oxide fuel cell (IT-SOFC) the Ruddlesden-Popper (RP) phases of LnSr3-xCaxFe3-yByO10 (Ln = La, Pr and Sm; x = 0-1.0; B = Ni, Co, and Cu; y = 0-1.5) was investigated and prepared by citric nitrate combustion method. Firstly, various Ca concentrations were added to the LaSr3-xCaxFe3O10-δ structure and it was found that the materials contained low Ca concentration provided the lower area specific resistance (ASR) and better electrochemical cell performance due to the enhance in conductivity and reduction of TEC. Secondly, the effect of Pr and Sm replacement …
Electrochemical Sensors For Human Papillomavirus Dna, C-Reactive Protein And Food Colorants Detections,
2017
Faculty of Science
Electrochemical Sensors For Human Papillomavirus Dna, C-Reactive Protein And Food Colorants Detections, Sakda Jampasa
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this dissertation, electrochemical sensors for determination of medical biomolecular markers and food colorants were developed. The research can be divided into two parts. In the first part, electrochemical sensors for determination of clinically important biomarkers were studied. There was two subprojects based on the target markers. The first subproject was the development of electrochemical sensor for the simultaneous detection of high-risk human papillomaviruses (HPV) DNA type 16 and 18 as biomarkers for cervical cancer. In this work, a highly specific pyrrolidinyl peptide nucleic acid was employed as DNA capture probe for both target DNAs. A second PNA probe labeled …
Fecu/Sio2 Catalysts Prepared By Autocombustion Method For Fischer-Tropsch Synthesis,
2017
Faculty of Science
Fecu/Sio2 Catalysts Prepared By Autocombustion Method For Fischer-Tropsch Synthesis, Suthasinee Pengnarapat
Chulalongkorn University Theses and Dissertations (Chula ETD)
The purpose of this study was to prepare iron-based catalysts supported on silica by autocombustion method for directly using for Fischer-Tropsch synthesis (FTS) without a reduction step. The effect of different citric acid (CA):iron nitrate (N) molar ratios (CA:N), acid types and SiO2 supports on the FTS performance of catalysts were investigated. The CA:N had an important influence on the formation of iron active phases (FexC) and FTS activity. Increasing the CA:N molar ratios up to 0.1 increased CO conversion of catalyst to 86.5%, which was then decreased markedly at higher CA:N molar ratios. An excess of CA resulted in …
Microencapsulation Of Eugenol In Polyelectrolyte Complexes Of Chitosan And Alginate,
2017
Faculty of Science
Microencapsulation Of Eugenol In Polyelectrolyte Complexes Of Chitosan And Alginate, Chamaiporn Supachettapun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Eugenol is widely used in several applications such as food activity packaging to prolong shelf life of fruits and vegetables because of its antimicrobial activity. However, eugenol is easy to decompose, leading to reduce its activity. In this work, we prepared eugenol loaded alginate-chitosan microparticles in order to solve this problem and controlled release essential oil using ionic gelation technique. The effect of concentration of eugenol (0.1-0.5 g), calcium chloride (0.5 – 8 %W/V) and surfactant (2-10% W/V) was investigated. The results showed that the optimum condition was eugenol 0.1 g, calcium chloride 2% W/V and surfactant 4% W/V. The …
Functional Surfactants In Lubricant For Microelectronic Industry,
2017
Faculty of Science
Functional Surfactants In Lubricant For Microelectronic Industry, Chuenkamol Khongphow
Chulalongkorn University Theses and Dissertations (Chula ETD)
The functional surfactants, as the additional additives in the standard glycol-based lubricant were studied and optimized to reduce and protect the surface of the magnetic read/write head from deposited contaminants during the lapping processes. The scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS) results identified that the black contamination was bismuth (Bi) particles, came out from lapping plate during the lapping process due to wear. Selected surfactants exhibited the results in reduce and prevent the deposition of contaminants by forming the micelle beyond their critical micelle concentration (CMC) values. We found that both polyethoxylate alcohol (PEAL) and quaternary amines …
Synthesis Of Puerarin-Gold Nanoparticles For Transdermal Delivery,
2017
Faculty of Science
Synthesis Of Puerarin-Gold Nanoparticles For Transdermal Delivery, Kwanruen Chanpeng
Chulalongkorn University Theses and Dissertations (Chula ETD)
Puerarin (Pur) is herbal medicine that its inflammatory response but low solubility to reduce activity. Thus, the aim of this study was to synthesize q188-kappa-carrageenan@Pur@gold nanoparticles (q188-κ-c@Pur@AuNPs) for enhancing solubility and transdermal delivery. The κ-c was synthesized by quaternization of κ-c with 25% (v/v) q188, and the q188-κ-c @AuNPs was prepared by reducing HAuCl4 into AuNPs by using 25% (v/v) q188-κ-c as reducing and stabilizing agents. The highest degree of quaternization (%DQ) at 21.12% was found q188 addition, which was calculated from 1H NMR. The solubility of the q188-κ-c derivatives was 43.7±0.01%, which higher than κ-c. The size of synthesized …
Effect Of Corrosion Inhibitors In Lubricant For Electronic Industry,
2017
Faculty of Science
Effect Of Corrosion Inhibitors In Lubricant For Electronic Industry, Natclitta Maipul
Chulalongkorn University Theses and Dissertations (Chula ETD)
The selected functional polymers and fatty esters of poly)1-butene pyrrolidone) (PP) and propylene glycol monostearate (PG) were investigated and applied to use as the corrosion inhibitors for protecting material removal during read/write head lapping processes in hard disk drive (HDD) production. Potentiodynamic polarization measurement was used to study their corrosion behaviors against antiferromagnetic materials (IrMn) which are the most sensitive materials in the head structure. Corrosion rate of PP- and PG-modified lubricants were 0.086 and 0.084 Å/min, respectively, while standard lubricant was 0.111 Å/min. Moreover, the leaching out of metallic atoms was investigated by using inductively couple plasma mass spectroscopy …
Mixed Metal Oxide Catalysts Derived From Natural Sources For Production Of Fatty Acid Methyl Esters,
2017
Faculty of Science
Mixed Metal Oxide Catalysts Derived From Natural Sources For Production Of Fatty Acid Methyl Esters, Wayu Jindapon
Chulalongkorn University Theses and Dissertations (Chula ETD)
The objectives of this research are to study active sites of Ca-based catalysts derived from calcite (CaCO3) and dolomite (CaMg(CO3)2), and to clarify catalytic effect of MgO in the calcined dolomite as catalyst in transesterification of palm oil with methanol. Firstly, the effects of calcination temperature on evolution of MgO in dolomite and its catalytic activity were studied. The MgO containing in the calcined dolomite was not active, while the main active site was CaO. However, Ca and Mg mixed oxides obtained from calcined dolomite had higher activity than that prepared by physical mixing in which calcite and magnesite (MgCO3) …
Hydrodeoxygenation Of Oleic Acid On Unsupported Co-Mo And Ni-Mo Sulfide Catalysts,
2017
Faculty of Science
Hydrodeoxygenation Of Oleic Acid On Unsupported Co-Mo And Ni-Mo Sulfide Catalysts, Paphawee Sanggam
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this research, hydrodeoxygenation (HDO) of oleic acid (model compound of palm oil) over unsupported Co-Mo and Ni-Mo sulfide catalysts was carried out in Parr reactor to produce bio-hydrogenated diesel (BHD). The effects of reaction parameters: temperature, hydrogen pressure and the mole ratio of catalysts (Ni/(Ni+Mo) or Co/(Co+Mo)) on the conversion and product yields (mainly n-C17 and n-C18 hydrocarbons) were investigated to find the optimal conditions. The results showed that high pressure favored a HDO pathway, while high temperature strongly affected to decarboxylation and decarbonylation pathways. At optimal condition as temperature of 280oC, reaction times of 6 h and hydrogen …
Preparation Of Porous Carbon From Popped Rice Via Impregnated Water Coupled With Freeze-Drying Process,
2017
Faculty of Science
Preparation Of Porous Carbon From Popped Rice Via Impregnated Water Coupled With Freeze-Drying Process, Purichaya Nisawa-Anutaraphan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Activated carbon is an interesting material due to its high surface area derived from the highly porous structure. This material is suitable for using as a good adsorbent in various applications such as wastewater treatment, gas absorption and medicine. Production of activated carbon typically consists of two steps which are carbonization and activation. Agricultural wastes are used as carbon sources. This research focuses on the production of activated carbon from popped rice due to its appropriate chemical and structural characteristics, including highly starch content and connected-pore structure. Therefore, it should potentially be applied to produce activated carbon if the surface …
Photooxidation Of Organosulfur Compounds Catalyzed By Iodo-Bodipy Derivatives Under Visible Light Irradiation,
2017
Faculty of Science
Photooxidation Of Organosulfur Compounds Catalyzed By Iodo-Bodipy Derivatives Under Visible Light Irradiation, Piyamaporn Tangkasemsamran
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this work, iodo-BODIPY derivatives, I-GB, 3I-GB, I-RB, were synthesized and used as photocatalysts for oxidation of organosulfurs such as thiols and thioethers into the corresponding disulfides and sulfoxides, respectively, as well as compared with benchmark photocatalyst, rose bengal. All BODIPY derivatives were successfully synthesized in good yields (36-80%) via condensation reaction between 4-iodobenzaldehyde and corresponding pyrroles, followed by oxidation with DDQ and complexation with BF3.OEt2. Among all synthesized BODIPYs, 3I-GB and 3I-RB showed relatively low quantum yield (0.053-0.054) with high singlet oxygen generation efficiency under green LED suggesting that both of them could serve as good photocatalysts for sulfur …
Development Of Hydrogen Embrittlement Resisted Pd-Cu Alloy Membrane,
2017
Faculty of Science
Development Of Hydrogen Embrittlement Resisted Pd-Cu Alloy Membrane, Ratchaneekorn Chukiatthai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The development of PdCu alloy membranes that can be used in hydrogen separation at low working temperature (<300°C) without hydrogen embrittlement problem is a very attractive topic for the petrochemical industry. Pd–Cu membranes have been prepared by electroless plating and electroplating method on stainless steel support. The compositions of the PdCu alloy membranes were related to both the metal deposition rates. The appropriate annealing temperature of Pd–Cu membranes was found at 500 °C in argon atmosphere for 24 hours to form a complete alloy material. After thermal annealing, the Cu atom was distributed in Pd alloy membrane. The surface morphology, compositions, and crystallinity of the PdCu alloy membranes were characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). The best Pd contents in PdCu alloy membrane were about 84 and 75 %at. The hydrogen flux through the PdCu alloy membrane was measured at temperature 150 to 300 °C and differential pressure 0.5 to 2.5 bar for membrane stability. The hydrogen flux increased with increasing temperature and pressure. Adding Cu atoms in Pd can reduce strain in Pd lattice structure. Both PdCu alloy membrane can be operated at the lower temperature than a critical temperature of pure Pd membrane without hydrogen embrittlement.
Effect Of Metallocene Elastomers And Screw Speed On Polypropylene Compounds: Morphological And Physical Properties,
2017
Faculty of Science
Effect Of Metallocene Elastomers And Screw Speed On Polypropylene Compounds: Morphological And Physical Properties, Supot Ponhan
Chulalongkorn University Theses and Dissertations (Chula ETD)
Polypropylene (PP) which is widely used in automotive industries can replace engineering plastics and metals due to its outstanding mechanical properties, moldability and reduced weight. Some automotive parts such as bumper require higher impact resistance, which is the limited property of PP. The aim of this work is to improve the impact properties with balancing the stiffness-impact properties and the physical properties by blending PP with metallocence elastomer (POE) using a twin screw extruder. The effects of PP and POE type and blending ratio on the PP/POE blends properties and morphology was studied. The results showed that the impact strength …
Water/Dust Repellent Coatings For Wood Surface From 3-Aminopropyltriethoxy Silane-Quat 188,
2017
Faculty of Science
Water/Dust Repellent Coatings For Wood Surface From 3-Aminopropyltriethoxy Silane-Quat 188, Tanate Tubtimtong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Wetting and dust fouling on wood surface are big problems because of hydroxyl group and static charge on its surface. These problems could be minimized by surface modification which decreasing of hydroxyl groups and increasing of positive charges on wood surface. Many researches were reported about wetting issue but there were few studies directly involved to dust problem. In this research, 3-aminopropyltriethoxy silane (APTES) and 3-chloro-2-hydroxypropyltrimethyl ammonium chloride (Q188) were used to modify hydroxyl groups on wood surface to enhance wetting and dust repellency behavior of wood. The APTES-treated woods (AW) were prepared by immersion solution method and then were …
Molecular Dynamics Of Single-Wall Carbon Nanotube Wrapped With Polypropylene,
2017
Faculty of Science
Molecular Dynamics Of Single-Wall Carbon Nanotube Wrapped With Polypropylene, Wanwisa Panman
Chulalongkorn University Theses and Dissertations (Chula ETD)
Nowadays, the nanocomposite materials have been widely used in various applications due to their unique properties such as thermal and electrical properties. Polymer/carbon nanotube (CNT) nanocomposite is one the important nanocomposite materials that are manufactured for improving thermal conductivity and electrical properties of polymers. Unfortunately, polypropylene (PP)/CNT preparation is difficult because of CNT dispersion and aggregation. Accordingly, amylose (AMY) and chitosan (CS) are selected in the present study in order to demonstrate how AMY and CS could diminish such problems by non-covalent modification on outer surface of single-walled CNT using molecular dynamics (MD) simulations. The MD results reveal that AMY …
