The Antitumor Effects Of Plasma-Activated Saline On Muscle-Invasive Bladder Cancer Cells In Vitro And In Vivo Demonstrate Its Feasibility As A Potential Therapeutic Approach,
2021
Old Dominion University
The Antitumor Effects Of Plasma-Activated Saline On Muscle-Invasive Bladder Cancer Cells In Vitro And In Vivo Demonstrate Its Feasibility As A Potential Therapeutic Approach, Hao Zhang, Jishen Zhang, Bo Guo, Hailan Chen, Dehui Xu, Michael G. Kong
Bioelectrics Publications
Muscle-invasive bladder cancer (MIBC) is a fast-growing and aggressive malignant tumor in urinary system. Since chemotherapy and immunotherapy are only useable with a few MIBC patients, the clinical treatment of MIBC still faces challenges. Here, we examined the feasibility of plasma-activated saline (PAS) as a fledgling therapeutic strategy for MIBC treatment. Our data showed that plasma irradiation could generate a variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in saline. In vivo tests revealed that pericarcinomatous tissue injection with PAS was effective at preventing subcutaneous bladder tumor growth, with no side effects to the visceral organs after …
Comprehensive Collagen Crosslinking Comparison Of Microfluidic Wet-Extruded Microfibers For Bioactive Surgical Suture Development,
2021
Old Dominion University
Comprehensive Collagen Crosslinking Comparison Of Microfluidic Wet-Extruded Microfibers For Bioactive Surgical Suture Development, Amrita Dasgupta, Nardos Sori, Stella Petrova, Yas Maghdouri-White, Nick Thayer, Nathan Kemper, Seth Polk, Delaney Leathers, Kelly Coughenour, Jake Dascoli, Riya Palikonda, Connor Donahue, Anna A. Bulysheva, Michael P. Francis
Bioelectrics Publications
Collagen microfiber-based constructs have garnered considerable attention for ligament, tendon, and other soft tissue repairs, yet with limited clinical translation due to strength, biocompatibility, scalable manufacturing, and other challenges. Crosslinking collagen fibers improves mechanical properties; however, questions remain regarding optimal crosslinking chemistries, biocompatibility, biodegradation, long-term stability, and potential for biotextile assemble at scale, limiting their clinical usefulness. Here, we assessed over 50 different crosslinking chemistries on microfluidic wet-extruded collagen microfibers made with clinically relevant collagen to optimize collagen fibers as a biotextile yarn for suture or other medical device manufacture. The endogenous collagen crosslinker, glyoxal, provides extraordinary fiber ultimate tensile …
Cardioporation Enhances Myocardial Gene Expression In Rat Heart,
2021
Old Dominion University
Cardioporation Enhances Myocardial Gene Expression In Rat Heart, Carly Boye, Sezgi Arpag, Nina Burcus, Cathryn Lundberg, Scott Declemente, Richard Heller, Michael Francis, Anna Bulysheva
Bioelectrics Publications
Damage from myocardial infarction (MI) and subsequent heart failure are serious public health concerns. Current clinical treatments and therapies to treat MI damage largely do not address the regeneration of cardiomyocytes. In a previous study, we established that it is possible to promote regeneration of cardiac muscle with vascular endothelial growth factor B gene delivery directly to the ischemic myocardium. In the current study we aim to optimize cardioporation parameters to increase expression efficiency by varying electrode configuration, applied voltage, pulse length, and plasmid vector size. By using a surface monopolar electrode, optimized pulsing conditions and reducing vector size, we …
The Hsp90 Inhibitor, Auy-922, Protects And Repairs Human Lung Microvascular Endothelial Cells From Hydrochloric Acid-Induced Endothelial Barrier Dysfunction,
2021
Old Dominion University
The Hsp90 Inhibitor, Auy-922, Protects And Repairs Human Lung Microvascular Endothelial Cells From Hydrochloric Acid-Induced Endothelial Barrier Dysfunction, Ruben M.L. Colunga Biancatelli, Pavel Solopov, Betsy Gregory, John D. Catravas
Bioelectrics Publications
Exposure to hydrochloric acid (HCl) leads acutely to asthma-like symptoms, acute respiratory distress syndrome (ARDS), including compromised alveolo-capillary barrier, and respiratory failure. To better understand the direct effects of HCl on pulmonary endothelial function, we studied the characteristics of HCl-induced endothelial barrier dysfunction in primary cultures of human lung microvascular endothelial cells (HLMVEC), defined the involved molecular pathways, and tested the potentially beneficial effects of Heat Shock Protein 90 (HSP90) inhibitors. HCl impaired barrier function in a time- and concentration-dependent manner and was associated with activation of Protein Kinase B (AKT), Ras homolog family member A (RhoA) and myosin light …
Stobe Photography Mapping Of Cell Membrane Potential With Nanosecond Resolution,
2021
Old Dominion University
Stobe Photography Mapping Of Cell Membrane Potential With Nanosecond Resolution, Allen S. Kiester, Bennett L. Ibey, Zachary N. Coker, Andrei G. Pakhomov, Joel N. Bixler
Bioelectrics Publications
The ability to directly observe membrane potential charging dynamics across a full microscopic field of view is vital for understanding interactions between a biological system and a given electrical stimulus. Accurate empirical knowledge of cell membrane electrodynamics will enable validation of fundamental hypotheses posited by the single shell model, which includes the degree of voltage change across a membrane and cellular sensitivity to external electric field non-uniformity and directionality. To this end, we have developed a high-speed strobe microscopy system with a time resolution of ~ 6 ns that allows us to acquire time-sequential data for temporally repeatable events (non-injurious …
The Role Of Reactive Oxygen Species In The Immunity Induced By Nano-Pulse Stimulation,
2021
Old Dominion University
The Role Of Reactive Oxygen Species In The Immunity Induced By Nano-Pulse Stimulation, Siqi Guo, Niculina I. Burcus, Megan Scott, Yu Jing, Iurii Semenov
Bioelectrics Publications
Reactive oxygen species (ROS) are byproducts of tumor cells treated with Nano-Pulse Stimulation (NPS). Recently, ROS have been suggested as a contributing factor in immunogenic cell death and T cell-mediated immunity. This research further investigated the role of NPS induced ROS in antitumor immunity. ROS production in 4T1-luc breast cancer cells was characterized using three detection reagents, namely, Amplex Red, MitoSox Red, and Dihydroethidium. The efficiency of ROS quenching was evaluated in the presence or absence of ROS scavengers and/or antioxidants. The immunogenicity of NPS treated tumor cells was assessed by ex vivo dendritic cell activation, in vivo vaccination assay …
Effect Of He Plasma Jet Versus Surface Plasma On The Metabolites Of Acute Myeloid Leukemia Cells,
2021
Old Dominion University
Effect Of He Plasma Jet Versus Surface Plasma On The Metabolites Of Acute Myeloid Leukemia Cells, Dehui Xu, Ning Ning, Yujing Xu, Wenjie Xia, Hai-Lan Chen, Michael G. Kong
Bioelectrics Publications
Cold atmospheric plasma, including plasma jet and surface plasma, can promote the apoptosis of cancer cells without causing significant damage to surrounding normal cells, which was hopeful to be applied to the clinical cancer therapy. However, experimental plasma devices used directly to clinical experiments has challenges in technology and methods, especially the difference in killing tumor cells efficiency of these two common plasma sources. Therefore, it is great necessity to explore the differences in treating tumors between different plasma sources. This paper achieved good killing efficiency by using two kinds of cold atmospheric plasma generating devices, namely plasma jet and …
Mise Au Point D’Une Méthode De Mesure Objective De L’Effet D’Occlusion Basée Sur La Voix,
2021
Institut de recherche Robert-Sauvé en santé et en sécurité du travail (IRSST)
Mise Au Point D’Une Méthode De Mesure Objective De L’Effet D’Occlusion Basée Sur La Voix, Hugues Nélisse, Hugo Saint-Gaudens, Franck Sgard, Olivier Doutres
Rapports de recherche scientifique
Lors de l’utilisation de protecteurs auditifs ou de prothèses auditives, le fait d’obstruer le canal auditif peut engendrer un effet indésirable et désagréable, appelé « effet d’occlusion », qui se manifeste notamment par la sensation que notre propre voix sonne différemment, avec de basses fréquences amplifiées, qu’elle est distordue. L’effet d’occlusion est souvent considéré comme une source d’inconfort qui entraîne parfois les utilisateurs de protecteurs ou de prothèses à mal les utiliser ou encore à simplement les retirer. Dans le cas des travailleurs, par exemple, le retrait des protecteurs auditifs peut les exposer à des niveaux de bruits potentiellement dangereux, …
The Acute Impact Of Stair Climbing On Vertical Jump Height,
2021
Eastern Illinois University
The Acute Impact Of Stair Climbing On Vertical Jump Height, Matthew Grossman
Masters Theses
Vertical jump (VJ) is a strong determinant of explosive power in the trained and untrained population. There is agreement among the literature that vertical jump training can be improved through progression in specific training related to vertical jump. For example, plyometrics is a widely studied training method for increasing VJ, and has been generally accepted method for increasing VJ. Within the literature, training methods are continually being studied to improve athletic performance. Exploration of improvements outside of resistance training can provide further insight on enchancing athletic performance. The present study examined the acute impact of stair climbing (SC) on VJ. …
Qualitative Fitting Characteristics Of Elastomeric Half Face-Piece Respirators Using Isoamyl Acetate Agent,
2021
Old Dominion University
Qualitative Fitting Characteristics Of Elastomeric Half Face-Piece Respirators Using Isoamyl Acetate Agent, Anahita Fakherpour, Mehdi Jahangiri, Sean Banaee
Community & Environmental Health Faculty Publications
To examine the fitting testing of elastomeric half face-piece respirators (EHRs), a total of 41 candidates were randomly assigned into seven EHRs equipped with organic vapor (OV) cartridges which were commonly used in the Iranian industrial workplaces. The qualitative fitting into the facial dimensions was assessed using the Allegro Isoamyl Acetate fit test kit. While the studied EHRs showed very low passing fit testing rates, the 3M, AoSafety (Medium), and AoSafety (Large) had the highest passing rates with 22.0%, 14.60%, and 9.76%, respectively. The AoSafety (All sizes) delivered a higher passing fit test rate than the 3M brand (29.30 vs. …
The Role Of Hamstring Contraction During Running: An Analysis,
2021
Liberty University
The Role Of Hamstring Contraction During Running: An Analysis, Theodore Hu
Senior Honors Theses
The hamstring muscle group, spanning the posterior aspect of the proximal lower limb, generates large amounts of contractile force during running. Researchers have examined this power generation within different phases of the stride cycle. Forces generated by the hamstrings hold implications for both running efficiency and the role of strengthening for injury prevention. The goal of this review is to examine the hamstring’s unique physiology as a bi-articular muscle, its role in running stride, various training philosophies, and the specific impact on running economy contributing to overall running performance. The summation of research presented shows that the hamstring muscle group …
Peculiarities Of Neurostimulation By Intense Nanosecond Pulsed Electric Fields: How To Avoid Firing In Peripheral Nerve Fibers,
2021
Old Dominion University
Peculiarities Of Neurostimulation By Intense Nanosecond Pulsed Electric Fields: How To Avoid Firing In Peripheral Nerve Fibers, Vitalii Kim, Emily Gudvangen, Oleg Kondratiev, Luis Redondo, Shu Xiao, Andrei G. Pakhomov
Bioelectrics Publications
Intense pulsed electric fields (PEF) are a novel modality for the efficient and targeted ablation of tumors by electroporation. The major adverse side effects of PEF therapies are strong involuntary muscle contractions and pain. Nanosecond-range PEF (nsPEF) are less efficient at neurostimulation and can be employed to minimize such side effects. We quantified the impact of the electrode configuration, PEF strength (up to 20 kV/cm), repetition rate (up to 3 MHz), bi- and triphasic pulse shapes, and pulse duration (down to 10 ns) on eliciting compound action potentials (CAPs) in nerve fibers. The excitation thresholds for single unipolar but not …
The Effect Of Endogenous Ligands Of The Aryl Hydrocarbon Receptor On Antibody Expression In A Human B-Cell Model,
2021
Wright State University
The Effect Of Endogenous Ligands Of The Aryl Hydrocarbon Receptor On Antibody Expression In A Human B-Cell Model, Valerie Benedict
Browse all Theses and Dissertations
Small changes in antibody expression have broad impacts on human health and disease. An environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces profound immune suppression in animal models in an aryl hydrocarbon receptor (AhR)-mediated fashion. In a human Burkitt's lymphoma cell line (CL-01), TCDD inhibited IgG secretion and differentially impacted Ig heavy chain expression. In addition to TCDD, the AhR has several endogenous, dietary, and bacterial ligands such as 6-formylindolo[3,2-b]carbazole (FICZ) and indole, which are present within the human body at varied concentrations (0.30 mM- 6.64 mM). Both FICZ (100 nM) and indole (100 µM) significantly reduced IgG secretion. Overall antibody secretion is …
Structual Analysis Of The Cl-Par-4 Tumor Suppressor As A Function Of Ionic Environment,
2021
Old Dominion University
Structual Analysis Of The Cl-Par-4 Tumor Suppressor As A Function Of Ionic Environment, Krishna K. Raut, Komala Ponniah, Steven M. Pascal
Chemistry & Biochemistry Faculty Publications
Prostate apoptosis response-4 (Par-4) is a proapoptotic tumor suppressor protein that has been linked to a large number of cancers. This 38 kilodalton (kDa) protein has been shown to be predominantly intrinsically disordered in vitro. In vivo, Par-4 is cleaved by caspase-3 at Asp-131 to generate the 25 kDa functionally active cleaved Par-4 protein (cl-Par-4) that inhibits NF-κB-mediated cell survival pathways and causes selective apoptosis in tumor cells. Here, we have employed circular dichroism (CD) spectroscopy and dynamic light scattering (DLS) to assess the effects of various monovalent and divalent salts upon the conformation of cl-Par-4 in vitro. We have …
Acoustic Detection Of Developmental Dysplasia Of The Hip In Models Representing Neonates,
2021
University of Central Florida
Acoustic Detection Of Developmental Dysplasia Of The Hip In Models Representing Neonates, Pinak Raodeo
Honors Undergraduate Theses
Developmental dysplasia of the hip (DDH) is a condition that involves the dislocation of the head of the femur in the acetabulum of the pelvic bone. Although it may not interfere with a child's range of motion during infancy, DDH can cause various effects over time such as joint pain, abnormal gait, and even paralysis. It is crucial to catch this phenomenon early on so that permanent disability is not introduced to the patient. In this study, an excitation device was used to send a broadband frequency signal through a hip joint simulated by a 3D printed bone apparatus consisting …
Accumbens Cholinergic Interneurons Mediate Cue-Induced Nicotine Seeking And Associated Glutamatergic Plasticity,
2021
Arizona State University
Accumbens Cholinergic Interneurons Mediate Cue-Induced Nicotine Seeking And Associated Glutamatergic Plasticity, Jonna M. Leyrer-Jackson, Michael Holter, Paula F. Overby, Jason M. Newbern, Michael D. Scofield, M. Foster Olive, Cassandra D. Gipson
Family and Community Medicine Faculty Publications
Nicotine, the primary addictive substance in tobacco, is widely abused. Relapse to cues associated with nicotine results in increased glutamate release within nucleus accumbens core (NAcore), modifying synaptic plasticity of medium spiny neurons (MSNs), which contributes to reinstatement of nicotine seeking. However, the role of cholinergic interneurons (ChIs) within the NAcore in mediating these neurobehavioral processes is unknown. ChIs represent less than 1% of the accumbens neuronal population and are activated during drug seeking and reward-predicting events. Thus, we hypothesized that ChIs may play a significant role in mediating glutamatergic plasticity that underlies nicotine-seeking behavior. Using chemogenetics in transgenic rats …
Opioid Use Disorder: The Timeline For Medication Assisted Therapy,
2021
Arcadia University
Opioid Use Disorder: The Timeline For Medication Assisted Therapy, Alexander Cristofori
Capstone Showcase
Opioid Use Disorder is patterns of opioid use leading to withdrawal, giving up important life events in order to use opioids, and excessive time spent using opioids, to name a few diagnostic criteria. The clinical progression of the disorder involves periods of acute exacerbation and remission that are cyclic in nature. Treatment is most effective when it includes both pharmacological and psychosocial modalities, referred to as medication assisted therapy (MAT). Three drugs used commonly in MAT-based treatment for OUD from oldest to newest include Methadone, Buprenorphine-naloxone, and Naltrexone. Treatment program models that prioritize total abstinence from the addictive substance attached …
Anatomical Variations And Morphometric Properties Of The Circulus Arteriosus Cerebri In A Cadaveric Malawian Population,
2021
University of Malawi, Malawi
Anatomical Variations And Morphometric Properties Of The Circulus Arteriosus Cerebri In A Cadaveric Malawian Population, Charles Nyasa, Anthony Mwakikunga, Lackson H. Tembo, Charles Dzamalala, Amadi O. Ihunwo
Brain and Mind Institute
Background: Knowledge of the anatomy of the circulus arteriosus cerebri (CAC) is important in understanding its role as an arterial anastomotic structure involved in collateral perfusion and equalisation of pressure, and may explain observed variations in neurovascular disease prevalences across populations. This study was aimed at understanding the anatomical configuration and morphometric properties of the CAC in Malawian population. Materials and methods: Brains were collected from 24 recently-deceased black Malawian human cadavers during medico-legal autopsies. Photographs of the CACs were taken using a camera placed at a 30 cm height from the base of the brain. Whole-circle properties and segmental …
Predicting Total Length Of Fish In Minnesota Lakes Using Bony Structure Dimensions,
2021
Bemidji State University
Predicting Total Length Of Fish In Minnesota Lakes Using Bony Structure Dimensions, Jay Holland
Journal of Earth and Life Science
This study seeks to establish a relationship between a fish’s total length to the size of various bony structures of prey fishes. Three important prey fish species in Minnesota were selected for examination in this study, including Black Crappie Pomoxis nigromaculatus, Bluegill Lepomis macrochirus, and Yellow Perch Perca flavescens. Length and weight dimensions of each fish’s otoliths, cleithra, and vertebrae were associated to total lengths of the fish they came from. In many dietary studies, the need for these relationships is strongly related to the disfigurement or degradation of prey fish in stomach samples. With accurate enough regression equations, researchers …
Distinct Cellular Profiles Of Hif1a And Vegf Mrna Localization In Microglia, Astrocytes And Neurons During A Period Of Vascular Maturation In The Auditory Brainstem Of Neonate Rats,
2021
CUNY City College
Distinct Cellular Profiles Of Hif1a And Vegf Mrna Localization In Microglia, Astrocytes And Neurons During A Period Of Vascular Maturation In The Auditory Brainstem Of Neonate Rats, Daphne Chang, Quetanya Brown, Grace Tsui, Ye He, Jia Liu, Lingyan Shi, Adrián Rodríguez-Contreras
Advanced Science Research Center
Defining the relationship between vascular development and the expression of hypoxia-inducible factors (Hifs) and vascular endothelial growth factor (Vegf) in the auditory brainstem is important to understand how tissue hypoxia caused by oxygen shortage contributes to sensory deficits in neonates. In this study, we used histology, molecular labeling, confocal microscopy and 3D image processing methods to test the hypothesis that significant maturation of the vascular bed in the medial nucleus of the trapezoid body (MNTB) occurs during the postnatal period that precedes hearing onset. Isolectin-B4 histochemistry experiments suggested that the MNTB vasculature becomes more elaborate between …
