Neuroscience and Neurobiology Commons^™

The Influence Of Valence On Action Control: A Comparison Of Simon Effects In The Extrinsic Affective Simon Task (East) And The Spatial Simon Task, Yaara Aleissa

College of Arts & Sciences Senior Theses

People adjust their thoughts and actions in response to a changing environment every day and emotions may influence these actions. Understanding how emotion impacts action control in situations where emotion is central to the action (task-relevant) compared to when it is present in the environment as distracting information (task-irrelevant) is essential. Currently it is unclear when emotion most significantly modulates action control. This study seeks to compare how valence (positive versus negative emotion) impacts action control performance when valence is task-relevant and task-irrelevant by using two modified versions of the Simon conflict task.

The Simon task is a paradigm to …

Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier

Honors Theses

This study explores how the tempo of music, measured in beats per minute (BPM), affects dogs' heart rate and oxytocin levels, adding to past research that shows that music affects physiological and behavioral states in dogs. Being able to understand these effects holds practical implications for improving overall well-being in canines in environments such as homes, shelters, and clinical environments. Using a within-subjects design, the dogs were exposed to slow and fast versions of one instrumental track, along with a white noise baseline condition before each randomized condition. Heart rate was recorded continuously throughout the session, and oxytocin saliva samples …

Expanding Access To Noninvasive Spinal Neuromodulation For Pediatric Spinal Cord Injury Rehabilitation: A Comparative Device Study, Alice E C Palmer

College of Arts & Sciences Senior Theses

Paralysis following spinal cord injury (SCI) has traditionally been considered permanent due to the disruption of supraspinal input to spinal motor circuits. However, growing evidence suggests that spinal networks below the level of injury retain intrinsic motor-generating capabilities through central pattern generators. Transcutaneous spinal cord stimulation (scTS) is a noninvasive neuromodulation technique that delivers electrical stimulation through the skin to reactivate these spinal networks. Although prior studies demonstrate that scTS delivered through research-grade devices is safe and effective in adults and children with chronic SCI, clinical translation remains limited by lack of FDA-approved systems, high costs, and restricted access to …

Using A Toxic Aging Coin To Assess Hexavalent Chromium Neurotoxicity: Impact Of Cr(Vi) On The Blood-Brain Barrier, Liam Buchanan

College of Arts & Sciences Senior Theses

The US population faces simultaneous public health threats from both aging and environmental pollutants. Chief among these is hexavalent chromium [Cr(VI)], whose toxic effects in peripheral tissues are well-established but its effects on the brain are still understudied. Following previous results indicating hippocampal metals dyshomeostasis after Cr(VI) exposure, we aim to assess Cr(VI)’s impact on the blood-brain barrier (BBB), a key structure for brain protection. We exposed young, middle-aged, and geriatric rats of both sexes to low levels of Cr(VI) in their drinking water for 90 days, after which we used immunofluorescence and confocal microscopy to analyze tissues for evidence …

Revised - The Role Of Gad67/65 In The Therapeutic Effects Of Ketamine And Fluoxetine In Fear-Conditioned Mice, Alex G. Ottimer

All NMU Master's Theses

Post-traumatic stress disorder (PTSD) is a chronic psychiatric disorder and the most common disorder following exposure to a traumatic event. The most commonly used drugs in PTSD treatment are selective serotonin reuptake inhibitors (SSRIs), though only about 50% of patients respond to this treatment. Previous work in our lab demonstrated the combination of chronic fluoxetine (FLX) and acute ketamine treatment decreased fear response in a contextual fear paradigm in male C57/BL mice. In rodents, fear conditioning (FC) is commonly used in the literature to induce behaviors related to PTSD. The mammalian central fear memory circuit involves a dynamic interplay between …

Adventitial Macrophage Accumulation Impairs Perivascular Nerve Function In Mesenteric Arteries With Inflammatory Bowel Disease, Elizabeth A. Grunz, Benjamin W. Jones, Olubodun Michael Lateef, Sidharth Sen, Katie Wilkinson, Trupti Joshi, Erika M. Boerman

Biomedical Sciences

Introduction: Inflammatory bowel disease involves aberrant immune responses and is associated with both cardiovascular disease risk and altered intestinal blood flow. However, little is known about how inflammatory bowel disease affects regulation of perivascular nerves that mediate blood flow. Previous work found perivascular nerve function is impaired in mesenteric arteries with Inflammatory bowel disease. The purpose of this study was to determine the mechanism of impaired perivascular nerve function.

Methods: RNA sequencing was performed on mesenteric arteries from IL10−/− mice treated with H. hepaticus to induce disease (inflammatory bowel disease) or left non-gavaged (Control). For all other studies, Control and …

Effects Of Vitamin E Deficiency On Anxiety-Like Behavior In Zebrafish With Distinct Stress Coping Styles, Areem Zahid

Theses/Capstones/Creative Projects

Stress and anxiety disorders affect millions worldwide and represent a significant public health burden. Individual differences in stress coping style and antioxidant regulation contribute to variability in stress responsiveness and anxiety-related behaviors. Oxidative imbalance has been implicated in the neurobiology of anxiety, but its interaction with inherent stress coping phenotypes remains unclear. This study examined whether long-term dietary vitamin E deficiency alters anxiety-like behaviors in zebrafish selectively bred for proactive and reactive stress coping styles. Adult zebrafish were maintained on either a vitamin E–sufficient or vitamin E–deficient diet for five months, followed by behavioral assessment using the novel tank diving …

Preclinical Animal Modeling To Investigate Redox-Mediated Molecular Dysfunction In Traumatic Brain Injury, Brandon Z. Mcdonald

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Impacts associated with traumatic brain injury (TBI) induce a multitude of neurological morbidities mediated by a spread of biochemical dysfunction. Redox stress is a significant contributor to TBI pathophysiology and several redox stress markers have substantial evidence to support their role in diagnosing preclinical and clinical TBI. However, there is a critical need to verify their translational utility through correlations with behavioral, imaging, and molecular modalities. In chapter two, we examined the therapeutic efficacy of an antioxidant treatment (NPC3) using a controlled cortical impact (CCI) mouse model of TBI. NPC3 alleviated glial expression and reactivity while influencing the expression of …

Long-Term Stimulant Use Is Associated With Normalized White Matter Integrity In The Vta-Nacc Pathway In Adolescents, Isaac T. Arsenault

Biological Sciences Undergraduate Honors Theses

Medical practitioners are diagnosing Attention-Deficit/Hyperactivity Disorder (ADHD) at a high and ever-increasing rate. The use of stimulant medication to treat ADHD is also increasing and is associated with changes in the brain. Past research found that children with ADHD have lower volume in reward neurocircuitry, such as the ventral tegmental area (VTA) and the nucleus accumbens (NAcc). Research has also found that stimulant usage in those with ADHD might “normalize” the reward circuits. However, few longitudinal studies have directly tested the long-term effects of stimulant medication on brain development. Here we tested whether long-term stimulant use is associated with changes …

Neurobiological Mechanisms Of Attention In Adolescents, Virginia Clark

All Dissertations

Adolescence is a transformative developmental period characterized by profound biological, neurological, and behavioral changes. This dissertation investigates the neurobiological underpinnings of attention in adolescents, emphasizing how internal mechanisms influence and individually shape cognitive development through the endosystem. Rooted in the intersection of human development, learning sciences, and cognitive neuroscience, this work argues for a more integrated understanding of attention as a fundamental cognitive function for learning, one that is behaviorally seen but also biologically and neurologically driven. While developmental science has often emphasized external influences such as family, culture, and environment, this work centers on the adolescent’s internal biological systems …

Application Of Path Analysis To Determine The Impact Of Age And Sex On The Neural Circuits That Promote Nicotine Withdrawal Severity, Veronika Espinoza

Open Access Theses & Dissertations

The rising prevalence of electronic cigarette (e-cigarette) use presents a major public health concern, particularly in adolescent females who are most susceptible to nicotine use and its long-term negative health consequences. The goal of this dissertation was to utilize preclinical rodent models to understand how group differences in age and sex influence neural activation patterns in brain circuits believed to promote nicotine withdrawal. Prior pre-clinical work, conducted largely in male rodents, has established that there are two major brain pathways that modulate the behavioral effects of nicotine withdrawal. These include the mesolimbic and medial habenula-interpeduncular nucleus (MHb-IPN) pathways. To assess …

Novel Roles Of G-Beta-Gamma Signaling In Microtubule Dynamics And Neurodegeneration, Diana I. Olivas

Open Access Theses & Dissertations

Neurodegenerative disorders (NDDs), including Alzheimer's disease (AD), are characterized by progressive neuronal dysfunction, cytoskeletal disruption, and cognitive decline. Although Tau hyperphosphorylation and neurofibrillary tangle (NFT) formation are classical hallmarks of AD, emerging evidence suggests that microtubule (MT) dysfunction may precede and occur independently of Tau pathology. Understanding upstream regulators of MT dynamics is therefore critical for elucidating early mechanisms of neurodegeneration. Previous work from our laboratory demonstrated that G protein βγ (Gβγ) subunits directly interact with tubulin and promote MT assembly. However, the mechanism of Gβγ signaling in neuronal cytoskeletal stability and neurodegeneration remained poorly understood. The present study investigated …

Salience Network Circuit Dynamics In Distress Tolerance And Drug-Seeking Vulnerability, Marina Alicia Smoak

Open Access Theses & Dissertations

The psychological distress experienced during drug withdrawal can lead individuals with substance use disorder to abandon their goal of abstinence. Numerous human studies demonstrate that an inability to persist in challenging, goal-directed behavior while experiencing psychological distress is associated with multiple stages of substance use, including relapse. This behavioral construct is referred to as low distress tolerance (DT). Rodent models of DT similarly show that low DT predicts elevated cocaine relapse, particularly when DT is assessed following extended abstinence. This abstinence period corresponds with the incubation of craving, a phenomenon characterized by progressively increased cocaine-seeking over time. Importantly, relapse vulnerability …

Characterizing The Molecular Response Of Embryonic Zebra Finch To Acute Sound Playback, Saidat O. Adeniran-Obey Miss

All Theses

Auditory stimuli are important cues that influence developmental plasticity in animals and are important for development and adaptation to an ever-changing environment. In the zebra finch (Taeniopygia guttata), there is evidence of adaptive developmental reprogramming in response to a prenatal ‘heat call’. Embryos exposed to this heat call exhibit changes in growth rate post-hatch, increased heat resistance, and enhanced reproductive success at maturity. However, it has long been assumed that embryonic zebra finches cannot respond to sound due to the immaturity of their auditory system. These findings raise the critical question of whether late-stage zebra finch embryos can, …

From Invisible Injury To Measurable Impact: Exploring Associations Between Peripheral Biomarkers Of Neuroinflammation And Mental Health Outcomes After Mtbi, Lauren Leggett Smith

Psychology Theses & Dissertations

Mild traumatic brain injury (mTBI), often referred to as a “silent epidemic,” accounts for the majority of traumatic brain injuries worldwide and is increasingly linked to persistent psychiatric outcomes. Despite its prevalence, mTBI remains understudied due to diagnostic challenges, underreporting, and misperceptions of its clinical significance. The present study examined whether peripheral biomarkers of neuroinflammation and astroglial injury—tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and glial fibrillary acidic protein (GFAP)— mediate associations between mTBI history and psychiatric symptom severity, including depression, anxiety, and posttraumatic stress disorder (PTSD). Participants were 138 traumaexposed adults recruited from an urban university and surrounding community, with …

Development Of A High-Throughput Framework For Studying Choice-Movement Dynamics In Freely Moving Rats Across Motivational And Pharmacological States, Atanu Giri

Open Access Theses & Dissertations

This thesis develops and applies high-throughput behavioral assays and analysis pipelines to study how external contingencies and internal state shape rodent decision-making. First, it introduces RECORD (Reward-Cost in Rodent Decision-making), a modular platform that combines 3D-printed arenas, microcontroller-based control, and closed-loop trial structure to deliver graded rewards and costs in a foraging-like environment. RECORD supports scalable data collection across multiple arenas and a software stack for parsing, databasing, and extracting spatiotemporal behavioral features and psychometric choice functions. Using this framework, the thesis quantifies how animals integrate sucrose reward magnitude with aversive light cost, revealing structured individual differences and sex-dependent movement …

Mapping The Lateral Hypothalamic Area: Chemoarchitecture, Connectivity, And Activation In Motivated Learning And Reward, Vanessa Inez Navarro

Open Access Theses & Dissertations

The lateral hypothalamic area (LHA) is a large and complex hypothalamic region with diverse functions across four main rostrocaudal divisions. Despite its size and diversity, disagreements over how to define LHA subdivisions (e.g., Swanson atlas lists 17 subdivisions) make it challenging to fully characterize its structure and function. Across these subregions, neuronal connectivity varies, indicating they serve different behavioral functions. Consistent with this, particular LHA neuronal pathways have been linked to reward-related responses, emphasizing the region's roles in motivation, feeding, and cognition. Current approaches to classifying the LHA have primarily relied on neurochemical markers or gene-directed manipulations, often without integrating …

High-Resolution Mapping Of Brainstem Feeding Circuits: A Framework For Targeted Neuroscience Tools, Geronimo Tapia

Open Access Theses & Dissertations

The neural control of energy balance is mediated in part by distributed brainstem circuits that integrate metabolic, visceral, and hormonal signals to coordinate appropriate motivated behaviors and autonomic responses. Despite extensive study, the precise neuroanatomical architecture regulating metabolism remains incompletely defined within standardized atlas frameworks. This work addresses that gap through complementary studies examining an acute glycemic challenge methodology known to stimulate feeding, the acute delivery of an anorexigenic-associated neurohormone, and the chemoarchitecture of a major feeding-related region, the parabrachial nucleus. First, utilizing 2-deoxy-D-glucose (2-DG) induced glucoprivation, we map the rapid response of dorsal medullary neuronal populations with high spatial …

A Novel Humidity-Entrained Clock For Mosquito And Fly Activities, Shyh-Chi Chen

Academic Affairs

Light and temperature are known to be the most potent factors that can regulate (or entrain) the circadian clocks; however, the role of other environmental factors in the regulation of circadian rhythms remains largely unexplored. Humidity, in particular, is critical for terrestrial insects' survival and fitness. Insects may use the information of daily humidity fluctuation to avoid excess water loss or dehydration. This project investigated the mechanism underlying the circadian rhythms at cellular and behavior levels. We identified the key molecules and clock neurons responsible for the sensory input and the entrainment of the circadian clock, leading to a better …

A Case For Mtor Pathway Screening In Early Epilepsy Diagnosis, David Seamons

Student Works

Mutations in the mTOR pathway can give rise to epileptic seizures in patients. While a variety of antiepileptic drugs are available to treat epilepsy, a large proportion of patients will experience refractory seizures (Chen et al., 2018). Additionally, patients with mTOR-related epilepsy may experience reduced efficacy in traditional therapeutic options to treat seizures (French et al., 2016; Hadzsiev et al., 2023; Kato et al., 2022; Moloney et al., 2023). Mutations resulting in mTOR-related epilepsy are genetically inherited; however, some variability in family histories of epilepsy may be due to second-hit somatic mutations in focal areas of the brain (Dawson et …