Laboratory and Basic Science Research Commons^™

Novel Nanoformulation Of Piperlongumine Attenuates Pancreatic Tumor Desmoplasia And Modulates Tumor Immune Responses, Vivek K. Kashyap, Reinaldo Mandes, Neeraj Chauhan, Daniel Zubieta, Mohammed Sikander, Eswara N.H.K Ghali, Bilal B. Hafeez, Murali M. Yallapu, Subhash C. Chauhan

Research Symposium

Pancreatic cancer (PanCa) is characterized by a lack of early diagnosis, poor response to available therapeutic modalities, and chemoresistance. Gemcitabine (GEM) is currently considered the most effective therapy for PanCa; however, it shows only a marginal survival benefit of 6 months. This poor drug response has been attributed to desmoplasia, causes suboptimal drug delivery, alters tumor microenvironment (TME), which includes tumor surrounding blood vessels, fibroblasts, immune cells, extracellular matrix, and other signaling molecules and induces chemo-resistance in tumors. To overcome these existing issues associated with chemotherapy, the identification and development of novel therapeutic modalities are a pressing need. Piperlongumine (PL) …

Ormeloxifene-Mediated Attenuation Of Β-Catenin Signaling In Hepatocellular Carcinoma Progression, Mohammed Sikander, Shabnam Malik, Daniel Zubieta, Anyssa Rodriguez, Molly Vela, Sheema Khan, Fathi T. Halaweish, Murali M. Yallapu, Meena Jaggi, Subhash C. Chauhan

Research Symposium

Background: Hepatocellular carcinoma (HCC) is the most prevalent form of primary liver cancer, with approximately 41,000 new cases and 29,000 deaths annually in the U.S. The management of HCC poses significant challenges due to severe toxicity and inadequate responses to existing chemotherapeutic agents. Aberrant β-catenin signaling is recognized as a crucial factor influencing the differentiation grades of HCC patients. Therefore, there is an urgent need to identify more effective and non-toxic β-catenin inhibitors for cancer treatment. Notably, ormeloxifene (ORM), a clinically approved selective estrogen receptor modulator with a favorable therapeutic index, has demonstrated remarkable anticancer activity, making it a promising …

Investigating The Relationship Among Environmental Factors, Reproductive Status, And Stress In A Preclinical Model Of Endocrine Function In Women’S Translational Health, Bianca A. Camacho, Laura Emma Garcia, Raquel Villarreal, Mario Gil

Research Symposium

Background: The average menstrual cycle of a healthy adult woman is a duration of approximately 28 days, during which in the follicular phase (first 14 days) the progesterone hormone is at a stable level while estrogen increases to its peak level on day 14. Day 14 is known as ovulation, during which progesterone increases during its Luteinizing Hormone (LH) surge while estrogen decreases. Menses, known colloquially as a woman’s “period,” begins approximately at day 14 after ovulation and lasts an average of 7 days. The luteal phase, otherwise known as the secretory phase, lasts for the remaining 14 days until …

Transcription Factor Binding And Discovery Of Novel Gene In The Opportunistic Human Pathogen, Pseudomonas Aeruginosa, Alaina Westee

Symposium of Student Scholars

Pseudomonas aeruginosa (PA) is a gram-negative, ubiquitously-found bacterium that primarily causes nosocomial, or hospital-borne, infections within immunocompromised patients. Therefore, it has been deemed a critical priority pathogen by the World Health Organization (WHO) and the Center for Disease Control (CDC), generating a need for research on its fundamental biology. The Van Dyke laboratory focuses on proteins called transcription factors, which promote or repress gene expression to regulate an organism’s functioning. We are specifically studying the cadmium-responsive transcription factor, CadR, due to the importance of metals in virulence and the lack of information on PA’s response to metal fluctuations. We intend …

The Correlation Between Temporomandibular Disorder (Tmd) And Vitamin D Deficiency, Iris Kim, Claudia Freitas

Annual Research Symposium

No abstract provided.

An Approach To Estimate Angling Effort, Catch, And Harvest Of Burbot Lota Lota, Collin J. Mitchell

Biology Graduate Theses

Once commonly thought of as a nuisance, Burbot Lota lota are now being frequently targeted by anglers in Minnesota. However, the amount of angling effort directed at Burbot, the distribution of effort, catch, and harvest are unknown. Historically, creel surveys have been used to estimate angling effort, catch, and harvest. Additionally, digital cameras have been used independently and supplementally to estimate angling effort, catch, and harvest. In this study, we estimate angling effort, catch, and harvest of Burbot using creel survey techniques and motion-activated digital cameras on Bad Medicine Lake, Minnesota, while also investigating and exploring the limitations of these …

Ivy Proteins As Guardians Of Bacterial Cell Wall Integrity: Regulation Of Lytic Transglycosylases Explored, Aamna Aijaz, Trinity E. Alamutu, Vy T. Dao, Nathaniel R. Davis, Michael D. Landers, Nykolas K. Mackevicius, Liliana Ortiz, Irina F. Padilla-Montoya, Kacy J. Smith, Maria L. Snyder, Anthony J. Stefanie, Blaise M. Williams, Thomas C. Leeper

The Kennesaw Journal of Undergraduate Research

Lytic transglycosylases (LTs) are bacterial enzymes involved in biosynthesis and maintenance of the peptidoglycan cell wall. LTs are reported to be regulated by two paralogs of Inhibitor of Vertebrate Lysozymes: Ivyp1 and Ivyp2. This study investigated the regulatory dynamics between the soluble LT (SltB1), and Ivy paralogs as putative regulators in Pseudomonas aeruginosa (PA). The secondary objective was to delineate the critical roles played by a glutamic acid at position 135 in SltB1 and a histidine at position 62 in Ivyp1 in governing their molecular interaction through site directed mutagenesis. Isolated SltB1 shows no lytic activity in our assay, in …

Delphi-Driven Consensus Definition For Mesenchymal Stromal Cells And Clinical Reporting Guidelines For Mesenchymal Stromal Cell-Based Therapeutics, Laurent Renesme, Kelly D. Cobey, Manoj M. Lalu, Tania M. Bubela, Raghavan Chinnadurai, John De Vos, Rod Dunbar, Dean Fergusson, Daniel Freund, Jacques Galipeau

Office of the Provost

Background aims: Despite promising results in pre-clinical studies, mesenchymal stromal cells (MSCs) face significant challenges in clinical translation. A scoping review by our group highlighted two key issues contributing to this gap: (i) lack of a clear and consensus definition for MSCs and (ii) under-reporting of critical parameters in MSC clinical studies. To address these issues, we conducted a modified Delphi study to establish and implement a consensus definition for MSCs and develop reporting guidelines for MSC clinical studies.
Methods: A steering committee of 22 international experts, including stakeholders from different MSC research fields, participated in the three Delphi rounds. …

Attenuation Of Vitamin E On Haloxyfop-R-Methyl Ester-Induced Hepatic Oxidative Stress In Orally Exposed Rats., Omolara D. Falodun

STEM for Success Resources

No abstract provided.

How Did Covid-19 Impact Physiology Research Globally? – Lessons Learned And Future Recommendations, Jeet Patel, Santanu De

Mako: NSU Undergraduate Student Journal

The Coronavirus Infectious Disease 2019 (COVID-19) pandemic caused massive changes in the medical field across the globe. As the whole world moved from in-person to remote activities, students found themselves in a predicament where they were compelled towards schooling and work online from home. This would mean that all projects, research, tests, and meetings were conducted over Zoom or other online meeting platforms. Laboratories were shut down or only a limited number of people were allowed to enter to conduct the necessary research. This paper discusses how the COVID-19 pandemic affected research process and experiments within various aspects of the …

Evaluating The Condition Of Age-0 Burbot In Relation To Diet, Cian Verbridge

Journal of Earth and Life Science

Burbot are becoming more and more popular for angling and aquaculture. However, populations have been declining across the world. Very little information is available about their early life and their interactions in the ecosystem due to challenges in sampling. Condition estimates and diet metrics were used to better understand the status of burbot in Lake Bemidji. Using backpack electrofishing, 45 young of the year burbot were collected. The lengths ranged from 86 to 146 mm and the wet weights ranged from 4.29 to 24.36 g. The stomach contents were removed and stored in ethanol for later analysis. The burbot were …

Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez

Journal of Earth and Life Science

Wild rice (mannoomin; Zizania palustris) is an aquatic grain that grows in slow-moving rivers and shallow bays throughout northern Minnesota. In Headquarters Bay on Leech Lake, wild rice has served as a cultural staple for Indigenous communities for centuries. However, its population has declined in recent years due to multiple environmental issues. One potential contributor is Eurasian watermilfoil (Myriophyllum spicatum; EWM), an invasive species that forms dense mats capable of outcompeting native aquatic vegetation, including wild rice. EWM was first recorded on the southern shoreline of Leech Lake in 2005 and has since become widespread. This study …

The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei

Journal of Earth and Life Science

Zebra mussels Dreissena polymorpha are an aquatic invasive species that has been spreading quickly throughout Minnesota water bodies since 1989. Zebra mussels are known to change certain morphological conditions in lakes such as turbidity, phytoplankton abundance, and aquatic invertebrate populations. However, studies showing impacts of the infestation of zebra mussels on the health of a fish community have been hard to prove due to a lack of repeated surveys and a suite of other confounding variables such as natural and seasonal variability, plant community, and lake morphometry. Therefore, the objective of this study is to determine whether zebra mussels have …

Size Of Age-0 Sander Vitreus Pre And Post Dreissena Polymorpha Infestation On Brainerd And Bemidji Area Lakes, Quady Bernu

Journal of Earth and Life Science

Walleye Sander vitreus is the Minnesota state fish and a vital predator fish in lake ecology. Zebra mussels, Dreissena polymorpha were first found in Minnesota inland lakes in the 1980’s. These mussels have had huge impacts on water clarity as well as phytoplankton and zooplankton population levels which affect how age-0 fish feed, especially a low light feeding predator fish like walleye. A study took place on age-0 walleye size and it supported the conclusion that age-0 walleye were negatively impacted by zebra mussels in Minnesota’s large lakes. The goals of this study were to determine how the size of …

Microsatellites Reveal The Population Genetic Structure Of Yellow Perch In Minnesota, Megan Boche

Journal of Earth and Life Science

When analyzing a population’s genetic makeup, looking at variation of individuals and populations is a crucial factor. The management of yellow perch will be aided by this information particularly in regard to stocking or relocation. Microsatellite loci were used to analyze the genetic population structure of yellow perch Perca flavescens within and among 29 sampling areas throughout Minnesota’s basins. Seven microsatellite DNA loci were evaluated, with heterozygosities ranging from 0.41 to 0.78. The Lower Mississippi basin samples showed higher numbers of alleles per locus and greater expected heterozygosities compared to other basins, with Lake Pepin (PEP) exhibiting the highest average …

Aquatic Invertebrate Populations In Roadside Wetlands In Relation To Water Characteristics, Ellen P. Isakson

Journal of Earth and Life Science

The continual habitat destruction and draining of wetland systems makes the need to understand the ecology of roadside wetlands all the more necessary. Roadside wetlands provide valuable habitat to countless aquatic invertebrates, which can provide insight into the health of the wetland system due to their diversity. The objective of this study was to analyze the populations of aquatic invertebrates in roadside wetlands in relation to water characteristics such as pH, salinity, conductivity, water depth and temperature, and dissolved oxygen. This was done by collecting samples of invertebrate populations from roadside wetlands, in addition to data on water quality characteristics …

Enhancing The Mechanical Properties Of Sla 3d-Printed Bio-Resin From Linseed Oil Using Cellulose And Lignin Biopolymers, Chukwunonso A. Ikedionu

College of Graduate Studies: Theses & Dissertations

Abstract

This research explores the development of bio resin from linseed oil by the process of epoxidation and acrylation to produce acrylated epoxidized linseed oil (AELO). Lignin and cellulose biopolymers were added as reinforcement at varying weight fractions of 1 wt.%, 2.5 wt.%, and 5 wt.%, and their mechanical performance was compared against pure AELO. The formulations were printed using an Elegoo SLA 3D printer to create standardized test specimens like tensile bars, compression cylinders, and hardness blocks, which were all prepared according to the ASTM testing standard.

The results indicate that reinforcement effects depend on both filler type and …

The Impact Of Plant-Based Diets On Erythrocyte Function, Oxidative Stress, And Vascular Inflammation In Type 2 Diabetes Mellitus, Walter G. Craig

Cal Poly Humboldt theses and projects

Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder with diverse etiological contributors, characterized by insulin resistance, chronic inflammation, oxidative stress, and an increased risk of vascular complications. While much research has focused on plasma and serum markers of metabolic dysfunction, the role of erythrocytes in diabetic pathophysiology remains underexplored. This review examines the impact of dietary macronutrient composition, particularly plant-based diets, on erythrocyte function, oxidative stress, and vascular inflammation in T2DM. Red blood cells (RBCs) are not only oxygen carriers but also dynamic regulators of immune and inflammatory responses, with structural and functional alterations playing a critical role …

Developing New Methods For Quantifying Karenia Brevis In Florida Waters, Antonia N. Ferguson

Honors Undergraduate Theses

Harmful Algal Blooms (HABs), known as red tides, have long impacted Florida’s coastal ecosystems. Caused by the toxic dinoflagellate Karenia brevis, these blooms are named based on the characteristic red discoloration of coastal waters. Negative impacts of K. brevis range from fish kills, neurotoxic shellfish poisoning, and respiratory irritation in humans. Traditionally, K. brevis monitoring has been conducted weekly by Florida Fish and Wildlife Conservation Commission’s Fish and Wildlife Research Institute (FWC-FWRI) using light microscopy for cell identification and enumeration of collected water samples. However, this method is time-consuming, costly, and relies on deep taxonomic expertise for proper cell …

The Role Of Horizontal Gene Transfer In Antibiotic Resistance Amidst Prevalent Community And Healthcare-Associated Mrsa Rates, Gabrielle Nascimento

Honors College Theses

In recent decades, cases of community-acquired MRSA (CA-MRSA) and healthcare-associated MRSA (HA-MRSA) have been on the rise. The ability of antibiotic resistance to transfer between microorganisms greatly expands its threat to the effective treatment and containment of bacterial infections. The mecA gene is found on a mobile cassette within the Staphylococcal cell and encodes for PBP2a, an alternate version of penicillin-binding protein (PBP) with less binding affinity for β-lactam antibiotics, increasing resistance to the effects of antibiotics. The goal of this research is to investigate the horizontal transfer of the mecA gene between various Staphylococcus species as well as to …

Training Module And Assessment Strategy For Light Microscopy Skills Training In A Research Environment, Kristopher E. Kubow, Joseph A. Harsh

Faculty Scholarship

Training in specialized techniques and instrumentation is an important component of undergraduate and graduate education. While such training can occur in formal lab courses, it also occurs as students engage in research. Although training in a research lab setting is typically informal, implementing evidence-based practices from the classroom would be beneficial, particularly for learning complex techniques and instruments, for example, light microscopy. Using advanced microscopes for research is more complicated than using teaching microscopes in a course. In addition, microscopes are easy to misuse because they will generally always produce an image even if that image is suboptimal, misleading, or …

Comparative Dna Extraction Methods To Use For Lamp Assay As Molecular Diagnosis Of Human Schistosomes From Urine Samples, Brittany R. Pulkkila, Chummy Sikasunge, James Mwansa, Nilanjan Lodh

Clinical Lab Sciences Faculty Research and Publications

Background

Schistosomiasis in Africa is an ongoing public health problem that is caused by two major human species, Schistosoma mansoni and S. haematobium, which often cause concurrent infections. Due to the global goal of controlling or eliminating schistosomiasis as a public health problem, the issue of diagnostic sensitivity has become more critical in the assessment of program success. In that regard, the World Health Organization (WHO) has drawn attention to the need for field-applicable tests with high specificity and sensitivity.

Methods

To address this, we have evaluated the amplification of S. mansoni and S. haematobium cell-free repeat DNA by …

Journal Of Earth And Life Science, Academic Year 2024-2025, Megan Boche, Nathan D. Pyburn, Ben Hoover, Jayden R. Alsleben, Hector Montoya, Jr, Reed Cumberland, Rose Kuhlmann, Ray Anderson, Ellie Isakson, Seth Sisneros-Martinez, Zanden Mellen, Quady Bernu, Cian Verbridge, Benjamin D. Strei, Madelaine Adamich, Arian Garcia, Tegwen Romportl, Sandra J. Marcu, Bemidji State University

Journal of Earth and Life Science

No abstract provided.

Size Structure Of Black Crappie And Bluegill In Northern, Eastern, Western, And Southern Minnesota, Ray Anderson

Journal of Earth and Life Science

Fish size structure is an important factor in a fishery population. Panfish such as black crappie Pomoxis nigromaculatus and bluegill Lepomis macrochirus play a major role in the health of fisheries. Five lakes from southern, northern, eastern, and western Minnesota were selected for analysis. Data were collected using standard sampling methods, including gill nets, electrofishing, and fyke nets. Length-frequency distributions were created to assess size structure in each region. Relative abundance was measured using catch per unit effort. Data analysis included a one-way ANOVA to compare Proportional Size Distribution (PSD) values and the distribution of fish sizes among regions. Chi-square …

Comparison Of Hoop Nets To Trap Nets In Sampling Panfish, Jayden R, Alsleben

Journal of Earth and Life Science

Panfish including bluegill Lepomis macrochirus and black crappie Pomoxis nigromaculatus are gamefish species of significance found in Minnesota. Hoop nets and trap nets are two types of gear that can be used to sample these fish. Fisheries managers and anglers alike are interested in the best tactics to capture panfish. The behavior of fish, including bluegill and black crappie, change throughout the sampling season and make it difficult to assume accurate representations. Trap nets have historically been adequate in sampling near shore populations, but questions surrounding the gear have allowed other options to be pursued. The objective of this study …

The Relationship Between Plant Density And Microplastic Concentration In The Water In Lake Bemidji, Mn, Arian Garcia

Journal of Earth and Life Science

Microplastics, plastic particles smaller than 5 mm, are an increasing environmental threat, particularly due to their widespread distribution and toxicity. While much research has focused on larger freshwater and marine ecosystems, the presence and impacts of microplastics in smaller inland lakes remain less understood. This study investigates the relationship between plant density and microplastic concentrations in Lake Bemidji, a smaller freshwater lake. A total of 30 water samples were collected across varying vegetation conditions, ranging from open water (0%) to 90% plant coverage. Microplastics were identified and categorized into six morphological types: fibers, fragments, films, spheres/beads, and foam. The samples …

A Sample Of Fish Communities In Beaver Ponds Of Northern Minnesota, Reed Cumberland, Rose Kuhlmann

Journal of Earth and Life Science

The North American beaver Castor canadensis is an ecosystem engineer and a keystone species to the environment. Beaver inhabit ponds and construct dams to build ponds that provide habitat for certain fish. Little information is provided on the species of fish that live in beaver ponds. The objective of this study is to sample the fish communities in beaver ponds while comparing the effectiveness of two styles of minnow traps. Twelve beaver ponds were selected in the Bemidji area to be sampled. The quantity and species caught in each style of trap were recorded to compare effectiveness of each trap …

Does Depth Affect Zebra Mussel Length In Scalp Lake?, Zanden Mellen

Journal of Earth and Life Science

Since its first sighting in 1996, zebra mussels Dreissena polymorpha have been confirmed in hundreds of Minnesota lakes. Not only were they found in lakes, but they are also found in rivers too. With lack of effective treatments to impede their spread, zebra mussels continue to colonize new areas causing ecological as well as economic harm. The objective of this study was to see if there was a relationship between a zebra mussel length and the depth, they are found using shell length (mm) and depth (cm). The comparison of zebra mussel length and depth was done by performing a …

Age Class Is Not Related To Size Or Composition Of Home Ranges Among Suburban White-Tailed Deer During Parturition, Nathan D. Pyburn

Journal of Earth and Life Science

How white-tailed deer use the landscape in an urban environment can help us understand the effects of urbanization on deer populations. White-tailed deer form observable matriarchal hierarchies in studied populations. This study examines the matriarchal structure, home range size, and home range composition of 25 collared white-tailed deer within the city limits of Bemidji, MN. The results found no significant difference between age class and home range size or composition. However, movement metrics from yearling and mature age classes were more similar than the juvenile age class females. I propose this is because social acceptance between mature females was higher …

Relationship Of Utricle And Trap Door Size On Common Bladderworts Utricularia Macrorhiza, Tegwen Romportl

Journal of Earth and Life Science

The common bladderwort Utricularia macrorhiza is a carnivorous aquatic plant that captures organisms with its bladders. This study was aimed at setting the stage for future research which might determine if U. macrorhiza could be a biological method of control for invasive species. The objective of this study was to determine the relationship between utricle and trap door sizes and to gather data on the size ranges of these two morphological features. A regression analysis was used to obtain results which showed there was enough evidence to suggest that the size of bladderwort trap doors have a significant, positive relationship …