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Articles 1 - 13 of 13
Full-Text Articles in Laboratory and Basic Science Research
Increasing The Resilience Of Plant Immunity To A Warming Climate, Jong Hum Kim, Christian Castroverde, Shuai Huang, Chao Li, Richard Hilleary, Adam Seroka, Reza Sohrabi, Diana Medina-Yerena, Bethany Huot, Jie Wang, Sharon Marr, Mary Wildermuth, Tao Chen, John Macmicking, Sheng Yang He
Increasing The Resilience Of Plant Immunity To A Warming Climate, Jong Hum Kim, Christian Castroverde, Shuai Huang, Chao Li, Richard Hilleary, Adam Seroka, Reza Sohrabi, Diana Medina-Yerena, Bethany Huot, Jie Wang, Sharon Marr, Mary Wildermuth, Tao Chen, John Macmicking, Sheng Yang He
Biology Faculty Publications
Extreme weather conditions associated with climate change affect many aspects of plant and animal life, including the response to infectious diseases. Production of salicylic acid (SA), a central plant defence hormone, is particularly vulnerable to suppression by short periods of hot weather above the normal plant growth temperature range via an unknown mechanism. Here we show that suppression of SA production in Arabidopsis thaliana at 28 °C is independent of PHYTOCHROME B (phyB) and EARLY FLOWERING 3 (ELF3), which regulate thermo-responsive plant growth and development. Instead, we found that formation of GUANYLATE BINDING PROTEIN-LIKE 3 (GBPL3) defence-activated biomolecular condensates (GDACs) …
Laurier Biology Departmental Seminars Poster Colouring Book (Vol 1), Tristan A.F. Long
Laurier Biology Departmental Seminars Poster Colouring Book (Vol 1), Tristan A.F. Long
Biology Faculty Publications
Departmental seminar posters are largely utilitarian affairs – who is talking?, when are they speaking?, where is the seminar? (and maybe will there will be refreshments served?). While functional, they often fail to capture the beauty and excitement of their subject matter, and may fail to engage a wider audience. As the organizer of the Wilfrid Laurier University Department of Biology seminar series in 2014, on a lark, I started drawing posters that tried to illustrate our speaker’s research in a non-conventional manner, and hopefully appeal to those that might otherwise find attending a departmental seminar intimidating. Little did I …
Planaria: An Animal Model That Integrates Development, Regeneration And Pharmacology, Oné R. Pagán
Planaria: An Animal Model That Integrates Development, Regeneration And Pharmacology, Oné R. Pagán
Biology Faculty Publications
Although planarians are established model organisms in developmental biology and regeneration studies, in the last forty years or so, they have caught the attention of pharmacologists, especially to study the pharmacology of drugs of abuse. This review covers the following topics: some fundamentals of the history of animal models and planarians in biomedical research; an abbreviated story of systematic pharmacology research using planarians as a model organism; an example of how planarians are contributing to the search for compounds against acute cocaine toxicity; an analysis of the number of papers on planarians and pharmacological topics from 1900- 2016; some perspectives …
Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah Dewolf, Bruno Carneiro
Evaluation Of The Developmental Toxicity Of Lead In The Danio Rerio Body, Nicole M. Roy, Sarah Dewolf, Bruno Carneiro
Biology Faculty Publications
Lead has been utilized throughout history and is widely distributed and mobilized globally. Although lead in the environment has been somewhat mitigated, the nature of lead and its extensive uses in the past prohibit it from being completely absent from our environment and exposure to lead is still a public health concern. Most studies regarding lead toxicity have focused on the brain. However, little is found in the literature on the effects of lead in other tissues. Here, we utilize the zebrafish model system to investigate effects of lead exposure during early developmental time windows at 24, 48 and 72 …
Brief Embryonic Strychnine Exposure In Zebrafish Causes Long-Term Adult Behavioral Impairment With Indications Of Embyronic Synaptic Changes, Nicole M. Roy, Brianna Arpie, Joseph Lugo, Elwood Linney, Edward D. Levin, Daniel Cerutti
Brief Embryonic Strychnine Exposure In Zebrafish Causes Long-Term Adult Behavioral Impairment With Indications Of Embyronic Synaptic Changes, Nicole M. Roy, Brianna Arpie, Joseph Lugo, Elwood Linney, Edward D. Levin, Daniel Cerutti
Biology Faculty Publications
Zebrafish provide a powerful model of the impacts of embryonic toxicant exposure on neural development that may result in long-term behavioral dysfunction. In this study, zebrafish embryos were treated with 1.5mM strychnine for short embryonic time windows to induce transient changes in inhibitory neural signaling, and were subsequently raised in untreated water until adulthood. PCR analysis showed indications that strychnine exposure altered expression of some genes related to glycinergic, GABAergic and glutamatergic neuronal synapses during embryonic development. In adulthood, treated fish showed significant changes in swimming speed and tank diving behavior compared to controls. Taken together, these data show that …
The Effects Of Fluorescent Tracking Powder On Oxygen Consumption In Salamanders Using Either Cutaneous Or Bimodal Respiration, Sarah A. Orlofske, Kristine L. Grayson, William A. Hopkins
The Effects Of Fluorescent Tracking Powder On Oxygen Consumption In Salamanders Using Either Cutaneous Or Bimodal Respiration, Sarah A. Orlofske, Kristine L. Grayson, William A. Hopkins
Biology Faculty Publications
Fluorescent powder is gaining attention as an effective method for tracking terrestrial amphibian movements, particularly for species that are too small for conventional tracking equipment. The technique requires coating portions of an animal with fluorescent powder, releasing the animal, and following the trail of powder as it is progressively lost during movement. Recent studies have shown that fluorescent powder has no negative effects on survival or growth. However, a substance that coats the skin, a major respiratory organ in most amphibians, may have sublethal effects on performance and consequently behavior. We tested the effect of fluorescent powder application on the …
Horseshoe Crab Spawning Survey Protocol, Jennifer Mattei
Horseshoe Crab Spawning Survey Protocol, Jennifer Mattei
Biology Faculty Publications
The protocol lists supplies and clothing needed for the survey, as well as which data is to be collected and when and how it should be entered for the census of horseshoe crabs on the Recapture Data Sheet for 2009. Adopted from Cape Cod and Delaware Bay survey protocols.
Glow Sticks As Effective Bait For Capturing Aquatic Amphibians In Funnel Traps, Kristine L. Grayson, Andrew W. Roe
Glow Sticks As Effective Bait For Capturing Aquatic Amphibians In Funnel Traps, Kristine L. Grayson, Andrew W. Roe
Biology Faculty Publications
Funnel traps of various designs have been used to capture adults and larvae of aquatic amphibians (e.g., Buech and Egeland 2002; Richter 1995). Most studies use unbaited funnel traps to capture amphibians while others have used shrimp or salmon eggs (Adams et al. 1997). Light traps and light sticks are commonly used in studies of fish, particularly larvae (Doherty 1987; Marchetti et al. 2004), but have not been widely used to capture amphibians. Glow sticks have been mentioned briefly in the literature as a means to increase capture rates of aquatic amphibians (Smith and Rettig 1996), but no studies have …
A Switch From Polyandry To Serial Monogamy: Results From A Three Year Tagging Study Of Horseshoe Crabs In Long Island Sound, Jennifer Mattei Ph.D., Paul Goodell, Christine Depierro, Meghann Burke
A Switch From Polyandry To Serial Monogamy: Results From A Three Year Tagging Study Of Horseshoe Crabs In Long Island Sound, Jennifer Mattei Ph.D., Paul Goodell, Christine Depierro, Meghann Burke
Biology Faculty Publications
Part of an ongoing population study of the North American Horseshoe Crab, Limulus polyphemus, in Long Island Sound was completed during the 2003 – 2005 spawning seasons at Milford Point, in Milford, CT. Horseshoe crabs range more widely throughout Long Island Sound than expected and exhibit weak site fidelity. Animals originally tagged in Milford were found as far west as Stamford and as far east as Clinton, CT. during the 3 year study. Out of 522 female horseshoe crabs tagged in 2004 only 4 came back to spawn again at Milford Pt. in 2005. The sex ratios of tagged and …
Inhibition Of Axonal Growth By Brefeldin A In Hippocampal Neurons In Culture, Mark Jareb, Gary Banker
Inhibition Of Axonal Growth By Brefeldin A In Hippocampal Neurons In Culture, Mark Jareb, Gary Banker
Biology Faculty Publications
The outgrowth of neuronal processes involves a great increase in the surface area of the cell. The supply of membrane material necessarily must be coordinated with the demands for neurite growth. The selective growth of only one or two neurites at any given time during the development of polarity raises the possibility that the production of materials by the soma is limiting for growth (Dotti and Banker, 1987; Dotti et al., 1988; Goslin and Banker, 1990). To examine the role of the availability of membrane components during the development of polarity and axonal elongation, we treated neurons with brefeldin A, …
Altered Trafficking Of Mutant Connexin32, Suzanne M. Deschênes, Jessica L. Walcott, Tamara L. Wexler, Steven S. Scherer, Kenneth H. Fischbeck
Altered Trafficking Of Mutant Connexin32, Suzanne M. Deschênes, Jessica L. Walcott, Tamara L. Wexler, Steven S. Scherer, Kenneth H. Fischbeck
Biology Faculty Publications
We examined the cellular localization of nine different connexin32 (Cx32) mutants associated with X-linked Charcot–Marie–Tooth disease (CMTX) in communication-incompetent mammalian cells. Cx32 mRNA was made, but little or no protein was detected in one class of mutants. In another class of mutants, Cx32 protein was detectable in the cytoplasm and at the cell surface, where it appeared as plaques and punctate staining. Cx32 immunoreactivity in a third class of mutants was restricted to the cytoplasm, where it often colocalized with the Golgi apparatus. Our studies suggest that CMTX mutations have a predominant effect on the trafficking of Cx32 protein, resulting …
Connexin32 Is A Myelin-Related Protein In The Pns And Cns, Steven S. Scherer, Suzanne M. Deschênes, Yi-Tian Xu, Judith B. Grinspan, Kenneth H. Fischbeck, David L. Paul
Connexin32 Is A Myelin-Related Protein In The Pns And Cns, Steven S. Scherer, Suzanne M. Deschênes, Yi-Tian Xu, Judith B. Grinspan, Kenneth H. Fischbeck, David L. Paul
Biology Faculty Publications
We have examined the expression of a gap junction protein, connexin32 (Cx32), in Schwann cells and oligodendrocytes. In peripheral nerve, Cx32 is found in the paranodal myelin loops and Schmidt-Lanterman incisures of myelinating Schwann cells, and the levels of Cx32 protein and mRNA change in parallel with those of other myelin-related genes during development, Wallerian degeneration, and axonal regeneration. In the central nervous system, Cx32 is found in oligodendrocytes and their processes, but not in compact myelin, and the levels of Cx32 protein and mRNA increase during development in parallel with those of the other myelin genes. Thus, Cx32 is …
Increased Stem Cell Factor Release By Hemangioma-Derived Endothelial Cells, C. J. Meininger, Shannon Brightman, K. A. Kelly, B. R. Zetter
Increased Stem Cell Factor Release By Hemangioma-Derived Endothelial Cells, C. J. Meininger, Shannon Brightman, K. A. Kelly, B. R. Zetter
Biology Faculty Publications
Background: Capillary hemangiomas, the most common tumors in young children, consist of proliferating capillary vessels and endothelial cells. These tumors also contain large numbers of mast cells, compared with the normal surrounding skin or tissue. We have recently shown that stem cell factor (SCF), the gene product of the murine steel locus, can act as a chemoattractant for mast cells. In this study, we investigated whether SCF might be involved in the recruitment and maintenance of mast cells in hemangiomas. Experimental Design: Cultured endothelial cells derived from a murine hemangioma were compared with normal vascular endothelial cells for the ability …