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Laboratory and Basic Science Research Commons™
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Articles 1 - 30 of 117
Full-Text Articles in Laboratory and Basic Science Research
Fish Tissue Digestion And Microplastic Polymer Identification Protocol Using Open-Source Ftir Database, Shawn L. Kissinger, Vick-Ariel Privert, Jason Bystriansky, Kyle A. Grice
Fish Tissue Digestion And Microplastic Polymer Identification Protocol Using Open-Source Ftir Database, Shawn L. Kissinger, Vick-Ariel Privert, Jason Bystriansky, Kyle A. Grice
DePaul Discoveries
Microplastic (MP) contamination in aquatic ecosystems poses significant concerns for environmental and public health, as seafood represents a critical food source worldwide. Despite increasing evidence of MP presence in marine organisms, efficient and accessible detection methods remain essential for characterizing contamination patterns and informing regulatory frameworks. This study extracted MPs from brain, gill, intestine, liver, and muscle tissues of gilt-head bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), two commercially important species from the Gulf of Cádiz, Spain, using a NaOH–HNO₃ chemical digestion protocol. Isolated particles were characterized by Fourier Transform Infrared (FTIR) spectroscopy, with …
Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser
Enhancing Walleye (Ogaa) Recruitment: Evaluating Assisted Reproduction As A Mitigation Strategy, Douglass Keiser
Biology Graduate Theses
Over the past several decades, cold- and cool-water fish populations across the Upper Midwest, including Minnesota and Wisconsin, have shown troubling signs of decline. Among the most affected is the walleye/Ogaa (Sander vitreus), a species deeply embedded in the ecological balance, cultural identity, and economic vitality of the region, and central to the lifeways, treaty-reserved rights, and resource stewardship of Tribal Nations, including Ojibwe communities across the Upper Midwest. Despite ongoing stocking efforts and harvest regulations, many lake systems have experienced persistent declines in natural recruitment, prompting resource agencies to investigate the underlying causes. One such system is …
Emerald Bowfin Amia Ocellicauda Ecology In Three Minnesota Lakes: Abundance, Age Analysis, Diet Composition, And Stable Isotope Food Web, Griffin Blegen
Emerald Bowfin Amia Ocellicauda Ecology In Three Minnesota Lakes: Abundance, Age Analysis, Diet Composition, And Stable Isotope Food Web, Griffin Blegen
Biology Graduate Theses
The Emerald Bowfin Amia ocellicauda is a predatory fish species native to Minnesota that has often been discriminated against. This research expands ecological knowledge of Bowfin in Minnesota and provides population data to guide future management. Bowfin were sampled in the spring of 2024 and 2025 and a multi-census mark–recapture model was used to estimate abundance in three lakes. Estimated populations were 196 individuals in Little Toad Lake (95% CI: 116, 242) or 4.4 fish/littoral ha (95% CI: 2.7,5.7), 657 in Lake Sarah (95% CI: 436, 1014) or 4.2 fish/littoral ha (95% CI: 3.0, 6.7), and 612 in Gun Lake …
Trophic Structure And Mercury Bioaccumulation In Walleye And Yellow Perch In The Upper And Lower Red Lake Basins, Marissa Pribyl
Trophic Structure And Mercury Bioaccumulation In Walleye And Yellow Perch In The Upper And Lower Red Lake Basins, Marissa Pribyl
Biology Graduate Theses
Mercury is a persistent global contaminant that biomagnifies through aquatic food webs, with dietary and environmental factors serving as the primary drivers of accumulation in fishes. Trophic structure and methylmercury dynamics in Walleye (ogaa; Sander vitreus) and Yellow Perch (asaawens; Perca flavescens) were investigated in the Upper and Lower Red Lake basins in Red Lake, Minnesota, during 2024-2025. Diets of Walleye and Yellow Perch were assessed through stomach dissections, and tissue samples from both species were analyzed for total mercury concentrations. Additional analyses included shiners (gigoozens; Notropis spp., Hudsonius spp.) along with a variety of freshwater fish and …
Largemouth Bass Diet Changes Through The Summer In Central Minnesota, Ethan Kunz
Largemouth Bass Diet Changes Through The Summer In Central Minnesota, Ethan Kunz
Journal of Earth and Life Science
Largemouth Bass Micropterus nigricans is one of the most widespread fish species in the world, due in part to human introduction for angling. Largemouth Bass are opportunistic predators that will consume the largest prey they can often find; however, it has been shown that their diet mainly consists of invertebrates such as crayfish, with less focus on other fish species. This research aimed to determine what the Largemouth Bass diets in Central Minnesota consist of, and how they may change from the beginning of summer through the beginning of fall. A total of 174 Largemouth Bass were sampled from late …
Relationship Between Ratio Of Hybrid Sunfish To Their Parent Species And Latitude, Maxwell Kattner
Relationship Between Ratio Of Hybrid Sunfish To Their Parent Species And Latitude, Maxwell Kattner
Journal of Earth and Life Science
Hybrid sunfish of the Lepomis genus make up a generally small but notable portion of the population of sunfish in Minnesota lakes. Hybrid sunfish play a significant role in freshwater ecosystems across the United States. They are both morphologically and behaviorally distinct in some ways and similar in other ways to their parent species, causing them to both fill and compete with their parent species for niches occupied by multiple Lepomis species. Despite this, the ratio of hybrid sunfish to their parent species has never been properly explored. This study observed if the ratio of hybrid sunfish to their parent …
Effectiveness Of Walleye Stocking In Winter Kill Lakes, Tyler Haus
Effectiveness Of Walleye Stocking In Winter Kill Lakes, Tyler Haus
Journal of Earth and Life Science
Winter kill events, marked by extensive fish mortality due to hypoxic conditions under the ice, are a common phenomenon in shallow eutrophic lakes, especially in the northern lakes in the northern United States. These events can drastically alter fish communities and often result in the elimination of top predators. Though these lakes will typically slowly bounce back to what they were in a couple of years. This study evaluates the effectiveness of walleye, Sander vitreus, stocking in lakes after a winter kill. For this study, four known winter kill lakes that are consistently stocked with walleye were studied. Pre- …
Assessing Urban–Rural Differences In Largemouth Bass Abundance In Minnesota, Jonathan Gates
Assessing Urban–Rural Differences In Largemouth Bass Abundance In Minnesota, Jonathan Gates
Journal of Earth and Life Science
Urbanization can significantly alter freshwater ecosystems and influence sport fish populations. This study examined differences in largemouth bass Micropterus nigricans catch-per-unit-effort (CPUE) between urban and rural lakes in Minnesota and evaluated temporal changes between 2000–2010 and 2011–2021. Forty lakes (20 urban, 20 rural) were selected using Minnesota Department of Natural Resources fisheries survey data. Lakes were classified using NLCD 2019 land cover data, with ≥10% developed land designated as urban. Nonparametric analyses (Wilcoxon rank-sum and signed-rank tests) were used to assess spatial and temporal differences in CPUE. Results showed that rural lakes had consistently higher median CPUE than urban lakes …
Statewide Northern Pike Response To Minnesota’S 2018 Regulation Change, Elijah Wozniak
Statewide Northern Pike Response To Minnesota’S 2018 Regulation Change, Elijah Wozniak
Journal of Earth and Life Science
Management of northern pike (Esox lucius) is challenging because it attempts to balance ecological sustainability with angler interests. Northern pike often stunt their own growth by diminishing the size and abundance of prey fish, their food source. Anglers are often displeased when small pike occur in high population densities. In response to populations of small northern pike, the DNR implemented a new, statewide northern pike regulation in 2018. The objective of this study was to determine whether the regulation successfully changed the mean weight, length, and CPUE of northern pike in Minnesota. The secondary objective was to test …
Neonicotinoid Bioaccumulation In Bluegill And Bullhead Within Lake Julia And Lake Zumwalde, Jake Stuber
Neonicotinoid Bioaccumulation In Bluegill And Bullhead Within Lake Julia And Lake Zumwalde, Jake Stuber
Journal of Earth and Life Science
Brown Bullhead Ameiurus nebulosus, Yellow Bullhead Ameiurus natalis, and Bluegill Lepomis macrochirus are unique in their stark differences. Bullhead are scaleless bottom feeders that can tolerate high pollution and low O2 conditions. In contrast, Bluegill are scaled, aggressive nesters and are less tolerant of high pollutants or low-oxygen conditions. Among these pollutants include a class of chemicals called neonicotinoids (hereafter referred to as neonics). Neonics are a class of insecticides primarily used within agriculture and pest management. Due to the effectiveness, application has escalated on a global scale. They have been well documented by many toxicology assessments. …
Comparison Of Calcified Structures To Estimate Ages Of Northern Pike Esox Lucius In Various Lakes, Kaed Rauk
Comparison Of Calcified Structures To Estimate Ages Of Northern Pike Esox Lucius In Various Lakes, Kaed Rauk
Journal of Earth and Life Science
Northern pike Esox lucius are a dominant predatory fish in many aquatic systems. They can have significant impacts on the ecosystems they inhabit and are also a popular game fish among anglers. However, northern pike are a difficult species to age accurately. This study aimed to compare calcified aging structures to identify trends and determine which structure provides the most accurate age estimates. A total of 119 northern pike were sampled, and multiple structures from each fish were independently aged by two readers. The coefficient of variation (CV) was calculated for each structure to assess precision. Sectioned sagittal otoliths exhibited …
Size Structure Comparison Of Bluegill In Lakes With And Without Special Regulations, Jenna Lawin
Size Structure Comparison Of Bluegill In Lakes With And Without Special Regulations, Jenna Lawin
Journal of Earth and Life Science
Lots of individuals have mixed feelings about special regulations for all sorts of game fish species such as northern pike Esox lucius, crappie Pomoxis spp., sunfish Lepomis spp., and so on. One species commonly sought after by many is bluegill Lepomis macrochirus. Bluegill are often deemed easy to find, catch, and clean. This study looks at three different categories of lakes (all lakes contain bluegill populations). The three categories are lakes with a five sunfish limit, lakes with a ten sunfish limit, and lakes without any special regulations. This study provides valuable insight into the size …
An Approach To Estimate Angling Effort, Catch, And Harvest Of Burbot Lota Lota, Collin J. Mitchell
An Approach To Estimate Angling Effort, Catch, And Harvest Of Burbot Lota Lota, Collin J. Mitchell
Biology Graduate Theses
Once commonly thought of as a nuisance, Burbot Lota lota are now being frequently targeted by anglers in Minnesota. However, the amount of angling effort directed at Burbot, the distribution of effort, catch, and harvest are unknown. Historically, creel surveys have been used to estimate angling effort, catch, and harvest. Additionally, digital cameras have been used independently and supplementally to estimate angling effort, catch, and harvest. In this study, we estimate angling effort, catch, and harvest of Burbot using creel survey techniques and motion-activated digital cameras on Bad Medicine Lake, Minnesota, while also investigating and exploring the limitations of these …
Evaluating The Condition Of Age-0 Burbot In Relation To Diet, Cian Verbridge
Evaluating The Condition Of Age-0 Burbot In Relation To Diet, Cian Verbridge
Journal of Earth and Life Science
Burbot are becoming more and more popular for angling and aquaculture. However, populations have been declining across the world. Very little information is available about their early life and their interactions in the ecosystem due to challenges in sampling. Condition estimates and diet metrics were used to better understand the status of burbot in Lake Bemidji. Using backpack electrofishing, 45 young of the year burbot were collected. The lengths ranged from 86 to 146 mm and the wet weights ranged from 4.29 to 24.36 g. The stomach contents were removed and stored in ethanol for later analysis. The burbot were …
Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez
Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez
Journal of Earth and Life Science
Wild rice (mannoomin; Zizania palustris) is an aquatic grain that grows in slow-moving rivers and shallow bays throughout northern Minnesota. In Headquarters Bay on Leech Lake, wild rice has served as a cultural staple for Indigenous communities for centuries. However, its population has declined in recent years due to multiple environmental issues. One potential contributor is Eurasian watermilfoil (Myriophyllum spicatum; EWM), an invasive species that forms dense mats capable of outcompeting native aquatic vegetation, including wild rice. EWM was first recorded on the southern shoreline of Leech Lake in 2005 and has since become widespread. This study …
The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei
The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei
Journal of Earth and Life Science
Zebra mussels Dreissena polymorpha are an aquatic invasive species that has been spreading quickly throughout Minnesota water bodies since 1989. Zebra mussels are known to change certain morphological conditions in lakes such as turbidity, phytoplankton abundance, and aquatic invertebrate populations. However, studies showing impacts of the infestation of zebra mussels on the health of a fish community have been hard to prove due to a lack of repeated surveys and a suite of other confounding variables such as natural and seasonal variability, plant community, and lake morphometry. Therefore, the objective of this study is to determine whether zebra mussels have …
Size Of Age-0 Sander Vitreus Pre And Post Dreissena Polymorpha Infestation On Brainerd And Bemidji Area Lakes, Quady Bernu
Size Of Age-0 Sander Vitreus Pre And Post Dreissena Polymorpha Infestation On Brainerd And Bemidji Area Lakes, Quady Bernu
Journal of Earth and Life Science
Walleye Sander vitreus is the Minnesota state fish and a vital predator fish in lake ecology. Zebra mussels, Dreissena polymorpha were first found in Minnesota inland lakes in the 1980’s. These mussels have had huge impacts on water clarity as well as phytoplankton and zooplankton population levels which affect how age-0 fish feed, especially a low light feeding predator fish like walleye. A study took place on age-0 walleye size and it supported the conclusion that age-0 walleye were negatively impacted by zebra mussels in Minnesota’s large lakes. The goals of this study were to determine how the size of …
Microsatellites Reveal The Population Genetic Structure Of Yellow Perch In Minnesota, Megan Boche
Microsatellites Reveal The Population Genetic Structure Of Yellow Perch In Minnesota, Megan Boche
Journal of Earth and Life Science
When analyzing a population’s genetic makeup, looking at variation of individuals and populations is a crucial factor. The management of yellow perch will be aided by this information particularly in regard to stocking or relocation. Microsatellite loci were used to analyze the genetic population structure of yellow perch Perca flavescens within and among 29 sampling areas throughout Minnesota’s basins. Seven microsatellite DNA loci were evaluated, with heterozygosities ranging from 0.41 to 0.78. The Lower Mississippi basin samples showed higher numbers of alleles per locus and greater expected heterozygosities compared to other basins, with Lake Pepin (PEP) exhibiting the highest average …
Journal Of Earth And Life Science, Academic Year 2024-2025, Megan Boche, Nathan D. Pyburn, Ben Hoover, Jayden R. Alsleben, Hector Montoya, Jr, Reed Cumberland, Rose Kuhlmann, Ray Anderson, Ellie Isakson, Seth Sisneros-Martinez, Zanden Mellen, Quady Bernu, Cian Verbridge, Benjamin D. Strei, Madelaine Adamich, Arian Garcia, Tegwen Romportl, Sandra J. Marcu, Bemidji State University
Journal Of Earth And Life Science, Academic Year 2024-2025, Megan Boche, Nathan D. Pyburn, Ben Hoover, Jayden R. Alsleben, Hector Montoya, Jr, Reed Cumberland, Rose Kuhlmann, Ray Anderson, Ellie Isakson, Seth Sisneros-Martinez, Zanden Mellen, Quady Bernu, Cian Verbridge, Benjamin D. Strei, Madelaine Adamich, Arian Garcia, Tegwen Romportl, Sandra J. Marcu, Bemidji State University
Journal of Earth and Life Science
No abstract provided.
Size Structure Of Black Crappie And Bluegill In Northern, Eastern, Western, And Southern Minnesota, Ray Anderson
Size Structure Of Black Crappie And Bluegill In Northern, Eastern, Western, And Southern Minnesota, Ray Anderson
Journal of Earth and Life Science
Fish size structure is an important factor in a fishery population. Panfish such as black crappie Pomoxis nigromaculatus and bluegill Lepomis macrochirus play a major role in the health of fisheries. Five lakes from southern, northern, eastern, and western Minnesota were selected for analysis. Data were collected using standard sampling methods, including gill nets, electrofishing, and fyke nets. Length-frequency distributions were created to assess size structure in each region. Relative abundance was measured using catch per unit effort. Data analysis included a one-way ANOVA to compare Proportional Size Distribution (PSD) values and the distribution of fish sizes among regions. Chi-square …
Comparison Of Hoop Nets To Trap Nets In Sampling Panfish, Jayden R, Alsleben
Comparison Of Hoop Nets To Trap Nets In Sampling Panfish, Jayden R, Alsleben
Journal of Earth and Life Science
Panfish including bluegill Lepomis macrochirus and black crappie Pomoxis nigromaculatus are gamefish species of significance found in Minnesota. Hoop nets and trap nets are two types of gear that can be used to sample these fish. Fisheries managers and anglers alike are interested in the best tactics to capture panfish. The behavior of fish, including bluegill and black crappie, change throughout the sampling season and make it difficult to assume accurate representations. Trap nets have historically been adequate in sampling near shore populations, but questions surrounding the gear have allowed other options to be pursued. The objective of this study …
The Relationship Between Plant Density And Microplastic Concentration In The Water In Lake Bemidji, Mn, Arian Garcia
The Relationship Between Plant Density And Microplastic Concentration In The Water In Lake Bemidji, Mn, Arian Garcia
Journal of Earth and Life Science
Microplastics, plastic particles smaller than 5 mm, are an increasing environmental threat, particularly due to their widespread distribution and toxicity. While much research has focused on larger freshwater and marine ecosystems, the presence and impacts of microplastics in smaller inland lakes remain less understood. This study investigates the relationship between plant density and microplastic concentrations in Lake Bemidji, a smaller freshwater lake. A total of 30 water samples were collected across varying vegetation conditions, ranging from open water (0%) to 90% plant coverage. Microplastics were identified and categorized into six morphological types: fibers, fragments, films, spheres/beads, and foam. The samples …
A Sample Of Fish Communities In Beaver Ponds Of Northern Minnesota, Reed Cumberland, Rose Kuhlmann
A Sample Of Fish Communities In Beaver Ponds Of Northern Minnesota, Reed Cumberland, Rose Kuhlmann
Journal of Earth and Life Science
The North American beaver Castor canadensis is an ecosystem engineer and a keystone species to the environment. Beaver inhabit ponds and construct dams to build ponds that provide habitat for certain fish. Little information is provided on the species of fish that live in beaver ponds. The objective of this study is to sample the fish communities in beaver ponds while comparing the effectiveness of two styles of minnow traps. Twelve beaver ponds were selected in the Bemidji area to be sampled. The quantity and species caught in each style of trap were recorded to compare effectiveness of each trap …
Does Depth Affect Zebra Mussel Length In Scalp Lake?, Zanden Mellen
Does Depth Affect Zebra Mussel Length In Scalp Lake?, Zanden Mellen
Journal of Earth and Life Science
Since its first sighting in 1996, zebra mussels Dreissena polymorpha have been confirmed in hundreds of Minnesota lakes. Not only were they found in lakes, but they are also found in rivers too. With lack of effective treatments to impede their spread, zebra mussels continue to colonize new areas causing ecological as well as economic harm. The objective of this study was to see if there was a relationship between a zebra mussel length and the depth, they are found using shell length (mm) and depth (cm). The comparison of zebra mussel length and depth was done by performing a …
Relationship Of Utricle And Trap Door Size On Common Bladderworts Utricularia Macrorhiza, Tegwen Romportl
Relationship Of Utricle And Trap Door Size On Common Bladderworts Utricularia Macrorhiza, Tegwen Romportl
Journal of Earth and Life Science
The common bladderwort Utricularia macrorhiza is a carnivorous aquatic plant that captures organisms with its bladders. This study was aimed at setting the stage for future research which might determine if U. macrorhiza could be a biological method of control for invasive species. The objective of this study was to determine the relationship between utricle and trap door sizes and to gather data on the size ranges of these two morphological features. A regression analysis was used to obtain results which showed there was enough evidence to suggest that the size of bladderwort trap doors have a significant, positive relationship …
Movement And Activity Of Burbot, (Mizay; Lota Lota) In A Spring-Fed Minnesota Lake, Abigail M. Fountain
Movement And Activity Of Burbot, (Mizay; Lota Lota) In A Spring-Fed Minnesota Lake, Abigail M. Fountain
Biology Graduate Theses
Burbot (Mizay; Lota lota) are a relatively unmanaged freshwater fish, important in many local Indigenous cultures, but often overlooked or maltreated in research, conservation, and fishing practices. As such, relatively little research has been conducted on Burbot, despite ecological significance as an indicator species. A recent resurgence in popularity as a target species, however, has prompted new research to inform management policies. The objectives of this study were to summarize Burbot movement by identifying patterns in swimming activity and identify possible predictors of the movement observed. This study investigated the movements of 39 individuals implanted with acoustic transmitters in Bad …
An Edna Assessment Of The Diamond Darter (Crystallaria Cincotta) In Its Current And Historic Distribution Range, Cameron Moore Layne
An Edna Assessment Of The Diamond Darter (Crystallaria Cincotta) In Its Current And Historic Distribution Range, Cameron Moore Layne
Graduate Theses, Dissertations, and Problem Reports (ETD)
Environmental DNA (eDNA) can serve as a useful monitoring tool for cryptic and imperiled freshwater stream fishes. eDNA can fill knowledge gaps regarding species’ distribution, site abundance, and habitat usage. The last known stronghold of the Diamond Darter (Crystallaria cincotta) in the Elk River of West Virginia provides an excellent test case to examine the utility of this non-invasive method of surveying rare species. Diamond Darters are one of the most newly recognized freshwater fishes in North America and are among the most imperiled U.S. fishes, having been federally listed as endangered in 2013. The cryptic behavior and limitations of …
Informing Management Of American Shad (Alosa Sapidissima) In The Carolinas: Evaluating Novel Fish Passage Approaches And Acoustic Tracking Methods, Margaret Gaither
Informing Management Of American Shad (Alosa Sapidissima) In The Carolinas: Evaluating Novel Fish Passage Approaches And Acoustic Tracking Methods, Margaret Gaither
All Theses
Acoustic telemetry is a valuable tool for fisheries scientists to study the movements and migratory behaviors of aquatic organisms. A critical assumption of this method is that tagged fish exhibit similar behaviors and survival rates as their untagged counterparts. To investigate this, we conducted a 12-week laboratory study on adult American Shad (Alosa sapidissima) using gastroesophageal insertion of acoustic transmitters. We implanted 80 fish with dummy transmitters and monitored tag retention and survival. Our analysis, employing a mixed effects Cox’s proportional hazards model, revealed no significant differences in survival between tagged and control fish, regardless of sex. Additionally, …
Phenotypic Plasticity In Pigmentation: Investigating Coloration Patterns In Anablepsoides Hartii Population Responses To Environmental Variation, Jasdeep Kaur
2024 Spring Honors Capstone Projects - Archive
In this study, we investigated the color-changing capabilities of Trinidadian killifish, Anablepsoides hartii, through exposure to varied color conditions over defined periods of time. Our experiment aims to understand adaptive responses when killifish are subjected to different color backgrounds, exploring their mechanisms to change color. By subjecting the species to different environmental color variations, we sought to discern color change patterns, which could indicate adaptive responses related to camouflage, stress, behavior clues, and physiological adjustments. Carefully controlling environmental factors, we monitored and documented the fishes’ color changes, aiming to provide insights into the interplay between fish’s developmental plasticity and its …
The Effects Of Habitat Characteristics And Location On Brook Trout Size Distribution, Bryce Groves
The Effects Of Habitat Characteristics And Location On Brook Trout Size Distribution, Bryce Groves
Journal of Earth and Life Science
Adfluvial Brook Trout Salvelinus fontinalis are known to typically grow larger than riverine Brook Trout. The Kabekona River in Hubbard County, Minnesota exhibits suitable habitat for both fluvial and adfluvial Brook Trout populations. The objective of this study was to analyze the relationships between depth, stream width, canopy cover, water temperature, sediment size, and distance from Kabekona Lake on Brook Trout size distribution in the Kabekona River. Brook Trout (n=35) were angled from the Kabekona River from August 24 to September 26, 2023. Each fish was measured and released, with habitat metrics being recorded at time of release. ArcGIS was …