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Laboratory and Basic Science Research Commons™
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Articles 1 - 16 of 16
Full-Text Articles in Laboratory and Basic Science Research
Effect Of Partial Ablation Of Skeletal Dynamin Related Protein 1 On Mitochondrial Health, Inflammation, And Fibrosis In A Mouse Model Of Duchenne Muscular Dystrophy, Tessa L. Duzz
Graduate Masters Theses
Duchenne muscular dystrophy (DMD) is a progressive neuromuscular disorder characterized by muscle weakness, degeneration, fibrosis, and impaired muscle regeneration. Mitochondrial dysfunction is an early pathological feature of DMD and has been linked to excessive mitochondrial fission. Dynamin-related protein 1 (Drp1) is a central regulator of mitochondrial fission, a key process in regulating mitochondrial quality and function. Inhibition of Drp1-mediated mitochondrial fission has shown promise as a potential therapeutic target for alleviating pathology and dysfunction in dystrophic muscle. The objective of this study was to determine whether partial skeletal muscle-specific ablation of Drp1 could improve muscle pathology and mitochondrial health in …
The Isolation And Characterization Of Acidovorax And Brevundimonas Bacteriophages With Preliminary Biocontrol Study On Arabidopsis Thaliana, Minh Quynh N. Vu
The Isolation And Characterization Of Acidovorax And Brevundimonas Bacteriophages With Preliminary Biocontrol Study On Arabidopsis Thaliana, Minh Quynh N. Vu
Senior Theses
As antibiotic-resistance becomes an increasing global concern, researchers are looking for alternatives to combat phytopathogenic rhizobacteria – one of which is through the application of phage biocontrol. To elucidate the complex tripartite relationships between phage, bacteria and plant interactions, nine novel phages were isolated from potential phytopathogenic strains of Acidovorax (RBW13) and Brevundimonas (RBW18). These phages were isolated from root samples obtained from the Rocky Branch Creek and the Broad River located in Columbia, SC. Using Pulsed-Field Gel Electrophoresis (PFGE), we identified seven “medium sized” phages (~40 kbp), one “large-sized” phage (~200 kbp), and one “small-sized” phage ( < 25 kbp). Three phages were determined to be lambda-like due to their “ladder-building” patterns on PFGE. Although spot-tests revealed that all phages seemed to have broad host ranges that spanned over multiple genera, plaque-formations must be observed in future experiments to determine if these phages can infect their hosts productively. Preliminary in planta experiments were performed on Arabidopsis thaliana …
Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan
Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan
DePaul Discoveries
Contamination of oceans by microplastics (mm) currently poses a major threat to aquatic and terrestrial ecosystems worldwide. Recent attention towards this issue has raised questions about the extent to which microplastics have accumulated in the environment, and has led to an increase in studies on the effects of microplastics in various organisms. However, levels of contamination in protected natural areas are still largely unexplored, yet can offer an important empirical perspective on the issue. In addition, little is known about the potential effects of microplastics on behavior in the field. This research was conducted within the protected ACE Basin National …
Exploring Pedagogical Approaches: A Comparative Analysis Of Information Delivery Methods In Fish Dissection Instruction, Kiara Smidt
Honors Projects
The Covid-19 pandemic prompted a global shift to remote work and education, challenging traditional teaching methods. This research explores the effectiveness of audiovisual versus visual-only guides in teaching perch dissection anatomy, safety, and procedure. The study involves a cross-sectional experiment with students from an Introduction to Biology course at Bowling Green State University. Participants were divided into groups using either a video or a written guide, and their knowledge was assessed before and after the dissection. Results calculated through a Student’s t-test indicate no significant difference in overall effectiveness between the two methods, apart from labeling an anatomy diagram and …
Comparison Of Globalfiler Pcr Amplification Inhibition Due To Humic Acid And Canine Dna, Mckinzie Bundy
Comparison Of Globalfiler Pcr Amplification Inhibition Due To Humic Acid And Canine Dna, Mckinzie Bundy
Honors Projects
To obtain a DNA profile from a crime scene sample, the DNA must undergo amplification through the Polymerase Chain Reaction (PCR). The PCR process can be inhibited by factors that increase DNase I activity or by factors that interfere with Taq polymerase binding to the DNA. This study compares inhibition to the PCR process caused by dog DNA with inhibition caused by humic acid. The GlobalFiler PCR Amplification Kit was tested using mixtures of dog DNA and human control DNA and mixtures of humic acid and human control DNA. The profiles obtained from these mixtures were then analyzed for instances …
Laurier Biology Departmental Seminars Poster Colouring Book (Vol 1), Tristan A.F. Long
Laurier Biology Departmental Seminars Poster Colouring Book (Vol 1), Tristan A.F. Long
Biology Faculty Publications
Departmental seminar posters are largely utilitarian affairs – who is talking?, when are they speaking?, where is the seminar? (and maybe will there will be refreshments served?). While functional, they often fail to capture the beauty and excitement of their subject matter, and may fail to engage a wider audience. As the organizer of the Wilfrid Laurier University Department of Biology seminar series in 2014, on a lark, I started drawing posters that tried to illustrate our speaker’s research in a non-conventional manner, and hopefully appeal to those that might otherwise find attending a departmental seminar intimidating. Little did I …
Laboratory Rotations In Cell And Molecular Biology, Michael Cerbo
Laboratory Rotations In Cell And Molecular Biology, Michael Cerbo
Library Impact Statements
No abstract provided.
Environmental Health And Saafety For Scientists Bps 357x, Joanna Burkhardt
Environmental Health And Saafety For Scientists Bps 357x, Joanna Burkhardt
Library Impact Statements
No abstract provided.
Bioethics (Gcsu), Kasey Karen, Jennifer Townes
Bioethics (Gcsu), Kasey Karen, Jennifer Townes
Biological Sciences Grants Collections
This Grants Collection for Bioethics was created under a Round Ten ALG Textbook Transformation Grant.
Affordable Learning Georgia Grants Collections are intended to provide faculty with the frameworks to quickly implement or revise the same materials as a Textbook Transformation Grants team, along with the aims and lessons learned from project teams during the implementation process.
Documents are in .pdf format, with a separate .docx (Word) version available for download. Each collection contains the following materials:
- Linked Syllabus
- Initial Proposal
- Final Report
Ocean Acidification And Predator-Prey Relations: Correlating Disruption Of Predator Avoidance With Chemosensory Deficits, Alexandra Fw Sidun, William G. Wright
Ocean Acidification And Predator-Prey Relations: Correlating Disruption Of Predator Avoidance With Chemosensory Deficits, Alexandra Fw Sidun, William G. Wright
Student Scholar Symposium Abstracts and Posters
One of the most destructive effects of global climate change is the increased carbon sequestering and consequential acidification of our world’s oceans. The impacts of ocean acidification on marine organisms are still relatively unknown, especially effects on behavioral ecology. Avoiding predation has emerged from recent behavioral ecology literature as a critical feature in the life history of a wide array of animal species; experiments on marine fishes suggest acidic water compromises their predator-avoidance abilities. Recent assays in our lab suggest predator-induced behavior is reduced by weakly acidic water. These experiments do not address the potential factor of generalized malaise caused …
Microbial Communities Of The Providence River, Jacqueline Kratch
Microbial Communities Of The Providence River, Jacqueline Kratch
Honors Projects in Science and Technology
The Providence River has been industrialized for over one-hundred years. Industries such as oil storage and metal recycling facilities have left high levels of pollutant metals, including lead (Pb), in the soil and water. The elevated Pb concentrations in these environments influence the selection of Pb resistance mechanisms in the bacterial community1. One mechanism of heavy metal resistance is the Pb efflux pump, consisting of proteins in the cell membrane that aid in the transport of Pb out of the cell2. In this study we investigated the co-occurrence of Pb efflux pumps and antibiotic efflux pumps in bacteria from Pb …
Receptor Of Advanced Glycation Endproducts (Rage) Is Positively Correlated With Tumor Necrosis Factor-Α In Adolescents With Obesity, Tasnim Rahman, Daniel H. Conrad, Anshu Gupta
Receptor Of Advanced Glycation Endproducts (Rage) Is Positively Correlated With Tumor Necrosis Factor-Α In Adolescents With Obesity, Tasnim Rahman, Daniel H. Conrad, Anshu Gupta
Undergraduate Research Posters
Introduction: Obesity in childhood is associated with an increased prevalence of diabetes and other traditional cardiometabolic risk factors, suggesting an epidemic of premature cardiovascular disease among today’s youth. Glycotoxins, known as advanced glycationend products (AGE’s), activating via the membrane-bound receptors (mRAGE), have been implicated in the pathophysiology of inflammation, (increased tumor necrosis factor-α [TNF-α ]), insulin resistance and vascular dysfunction in adults, but the role of RAGE in the early stages of metabolic disorders is unknown. In this study, we assessed relationship of cardiometabolicrisk factors, mRNA expression of TNF-α and RAGE in peripheral monocytes in adolescents with obesity.
Methods: Thirty …
It’S My Body: The Biomedical Ethics Of Cell And Organ Harvest, Christina Perri
It’S My Body: The Biomedical Ethics Of Cell And Organ Harvest, Christina Perri
Common Reading Essay Contest Winners
First Place
Hands-On Labs (Hol): Using Technology And Web-Based Resources To Transform A Traditional Lab-Based Biology Course Into A Distance Learning (Dl) Hybrid Course, Across Schools At Pace University., Erica Kipp
Cornerstone 3 Reports : Interdisciplinary Informatics
No abstract provided.
Hands-On Labs (Hol): Using Technology And Web-Based Resources To Transform A Traditional Lab-Based Biology Course Into A Distance Learning (Dl) Hybrid Course, Across Schools At Pace University., Erica Kipp
Cornerstone 3 Reports : Interdisciplinary Informatics
No abstract provided.
Building Better Scientists Through Cross-Disciplinary Collaboration In Synthetic Biology: A Report From The Genome Consortium For Active Teaching Workshop 2010, Michael J. Wolyniak, Consuelo J. Alvarez, Vidya Chandrasekaran, Theresa M. Grana, Andrea Holgado, Christopher J. Jones, Robert W. Morris, Anil L. Pereira, Joyce Stamm, Talitha M. Washington, Yixin Yang
Building Better Scientists Through Cross-Disciplinary Collaboration In Synthetic Biology: A Report From The Genome Consortium For Active Teaching Workshop 2010, Michael J. Wolyniak, Consuelo J. Alvarez, Vidya Chandrasekaran, Theresa M. Grana, Andrea Holgado, Christopher J. Jones, Robert W. Morris, Anil L. Pereira, Joyce Stamm, Talitha M. Washington, Yixin Yang
Biological Sciences Research
A common problem faced by primarily undergraduate institutions is the lack of funding and material support needed to adequately expose students to modern biology, including synthetic biology. To help alleviate this problem, the Genome Consortium for Active Teaching (GCAT) was founded in 2000 by Malcolm Campbell at Davidson College to bring genomics into the undergraduate curriculum. GCAT’s first tangible activity was to serve as a central clearinghouse both for the purchase and reading of DNA microarrays and for information on how to execute genomics experiments at undergraduate institutions. In response to the evolution of molecular biology in the last decade, …