Laboratory and Basic Science Research Commons^™

Effects Of Poor Quality Diets With Various Dietary Factors Added And Exposure To Cigarette Smoke On The Voluntary Alcohol Intakes Of Albino Rats, Bonnie Emmerson

Loma Linda University Electronic Theses, Dissertations & Projects

Studies were conducted utilizing albino rats to determine the relationship of voluntary alcohol consumption with various dietary additives and exposure to cigarette smoke in combination with poor quality diets. Male, Sprague-Dawley rats, from weanling age to 100 grams, were given the various experimental diets and a free fluid choice of ten per cent ethanol or tap water. Fluid intakes were recorded and analyzed statistically. Individual variation was common with a generally low level of alcohol consumption when rats were fed a poor quality diet of common United States foods. The addition of caffeinated beverages and/or spices and relish caused increases …

Periosteal Circulation And Its Contribution To The Nutrition Of Compact Bone, Jean Elaine Tassell

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Carbon Dioxide Fixation In Schistosoma Mansoni, Clint Earl Carter

Loma Linda University Electronic Theses, Dissertations & Projects

Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined …

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

Antibiotics And The Wasting Disease In Neonatally Thymectomized Rats, Douglas M. Grignon

Loma Linda University Electronic Theses, Dissertations & Projects

The administration of the antibiotic, Chloramphenicol, to pregnant rats just prior to, and for four days after delivery, diminishes the incidence of the so-called "Wasting Disease" in neonatally thymectomized rats.

A total of 50 rats were divided into the following groups:

1. Thymectomy only---nonmedicated 17 rats
2. Thymectomy only---medicated 12 rats
3. Thymectomy-adrenalectomy---nonmedicated 5 rats
4. Thymectomy-adrenalectomy---medicated 8 rats
5. Controls---nonmedicated 4 rats
6. Controls---medicated 4 rats

Chloramphenicol was administered in the drinking water to the mothers of groups 2, 4 and 6 for about 2 days prior to delivery and 4 days after. On the day of birth or within three days after birth, the …

Incidence Of Staphylococcus Aureus On Fomentation Covers, Yvonne Badgley Mcdaniel

Loma Linda University Electronic Theses, Dissertations & Projects

This study was to ascertain whether fomentation covers were contaminated with Staphylococcus aureus prior to being placed on a patient receiving a hydrotherapy treatment.

From a review of literature on the subject of the Staphylococcus aureus organism and from studies of hospital-acquired staphylococcal infection it appeared that: (1) researches had used various techniques for making cultures from fabrics, (2) the Staphylococcus aureus cultures may react in varying ways to the laboratory tests making the classification somewhat difficult, (3) the problem of hospital-acquired staphylococcal cross-infection was present, (4) the role played by the environment in hospital-acquired infections was controversial, and (5) …

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

Use Of Ion Exchange Resins As Buffers, Frederick B. Abeles

Journal of the Minnesota Academy of Science

No abstract provided.

Effect Of 9 Α-Fluorohydrocortisone Acetate On Gastric And Salivary Electrolytes Of Normal Fasting Subjects, Ardis Stern Beckner

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of 9α-FF on the Na:K ratio, chloride, and pH of saliva and gastric secretions, and on the total and free acid of gastric juice in normal human subjects was investigated. This study was designed to investigate whether conditions could be produced in the normal human subject similar to those present in patients with primary aldosteronism by using large doses of 9α-FF. August and others found a change in salivary electrolytes when cortisone, desoxycortisone, or ACTH were given to normal subjects. In these cases as in primary aldosteronism there was a decreased Na:K ratio and potassium excretion, with sodium …

The Effect Of Infection With Polyoma Virus On The Protein, Rna, And Dna Of Mouse Embryo Cell Cultures, Lawrence E. Mcconnehey

Loma Linda University Electronic Theses, Dissertations & Projects

In four separate experiments mouse embryo cell monolayers infected with polyoma virus and uninfected control mouse embryo cells were maintained in Eagle’s Minimum Essential Medium for periods of time ranging from 0 to 168 hours. The cell layers were removed from the glass by trypsinization. Samples of the cells were counted in a Coulter Counter and the remainder of the cells frozen after centrifugation, in a button. Later, the buttons were thawed and protein, RNA, and DNA analyses were performed on each sample. The micrograms per million cells of protein. RNA, and DNA were determined for each sample and plotted …

A Comparative Study Of The Growth Of Polyoma Virus In Two Secondary Cell Lines: Mouse Lymphoma And Normal Mouse Fibroblasts, Rheeta M. Stecker

Loma Linda University Electronic Theses, Dissertations & Projects

In comparing the growth of polyoma virus in secondary cell cultures of a mouse lymphoma (P388D₁, a known polyoma-supporting strain) and normal mouse fibroblasts (Earle’s L cells), the following observations have been made.

The L cell is capable of supporting the growth of the polyoma virus with reproduction and release of infectious particles. Viruses appear in the fluid from time to time over a period of several weeks. This probably represents a carrier state of the virus in L cells. Many of the L cells in infected cultures are not killed by the virus infection.

Virus particles released …

Natural History Of Thelazia Californiensis Price, 1930 (Nematoda: Thelaziidae), A Mammalian Eye Worm, Warren E. Parmelee

Loma Linda University Electronic Theses, Dissertations & Projects

Various factors—embryology, host associations, and seasonal changes In eye worn populations—tending to promote the propagation of T. califomlensls were studied.

The early embryology closely parallels that of other nematode species.

The mature embryos remain alive in saline at 35°C as long as 5 days. Dead embryos can be distinguished from living ones by virtue of the rapid staining by methylene blue by the former. Parturient female thelazias release embryos into saline, and embryos are found regularly in tear secretions of infected laboratory rabbits.

Ovic embryos possess an enveloping egg membrane containing chitin. On this account, this eye worm species is …

The Fern Prothallium As An Experimental Object For Morphogenetic Studies, Dorothy Anita Douglas

Theses & Honors Papers

No abstract provided.

Laboratory Or Busywork?, Albert Robinson Jr.

Journal of the Arkansas Academy of Science

No abstract provided.

The Effect Of Theelin Upon The Growth And Development Of The Genital Tracts Of The Female White Rat, Robert H. Grogan

Bachelors’ Theses

This paper has been designed to meet the requirements of a bachelor's thesis. The main substance of the thesis is a reiteration of some of the work done by other men in the field of ovarian hormones with some original work on the part of the author. I have endeavored to prove that theelin controls, to some extent at least, the development of the genital tracts of female white rats. Especial care was taken to follow out in detail the methods used by Doisy and his associates in similar experiments so that I would have a criterion by which to …