Laboratory and Basic Science Research Commons^™

Identification Of Eurycea Using Cytochrome B, Karis A. Myers

Senior Honors Theses

Genomic sequencing is a powerful tool that has many applications for research, one of which is in the field of taxonomy and the identification of species. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. The Polymerase Chain Reaction is used to amplify a target DNA sequence, and the protocol for this procedure must be optimized for the specific sequence of target DNA. Primers must also be designed and modified for …

Bringing Toxicology Into The 21st Century: A Global Call To Action, Troy Seidle, Martin Stephens

Experimentation Collection

Conventional toxicological testing methods are often decades old, costly and low-throughput, with questionable relevance to the human condition. Several of these factors have contributed to a backlog of chemicals that have been inadequately assessed for toxicity. Some authorities have responded to this challenge by implementing large-scale testing programmes. Others have concluded that a paradigm shift in toxicology is warranted. One such call came in 2007 from the United States National Research Council (NRC), which articulated a vision of ‘‘21st century toxicology” based predominantly on non-animal techniques. Potential advantages of such an approach include the capacity to examine a far greater …

Scientific Autonomy And The 3rs, Bernard E. Rollin

Experimentation Collection

No abstract provided.

Fisheries Research Report No.196 - Management Of Bioeroding Sponges In Wild Stocks Of Pinctada Maxima In Western Australia, Sabine Daume, Jane Fromont, Anthony M. Hart

Fisheries Research Reports

Final FRDC Report – Project 2005/074

The Western Australian fishery is the only remaining significant fishery for pearl oysters in the world and comprises most of the exported pearl product of Australia, currently worth around $125 million annually. The majority of pearl shells used to culture pearls come from the pearling beds in the inshore waters near Broome. The fishery for pearl oysters preferentially targets smaller ‘culture’ shell (4-5 years age class, 120mm - 160mm shell length) that are more suitable for growing pearls, leaving larger MOP oysters (6+ age class, 175mm+) on the fishing grounds.

Silver-lipped pearl oysters (Pinctada …

Curated Exhibit: Histological Colorations: Explorations Into The Human Form, Laura J. Hall

Posters and Presentations

This exhibit showcased mixed-media prints by artist Kathamann, inspired by her experience as a histology technician. The 26 prints, featured in the exhibit, offer colorful and whimsical interpretations of human histological slides, exploring the intricate beauty of the human body at a microscopic level. Curated by Laura J. Hall, MFA, the exhibit was displayed at the Domenici Center for Health Sciences Education. Kathamann’s work reflects both her scientific background and artistic vision, bringing a new perspective to the study of the human form. September 25, 2009 - August 31, 2010

The Effects Of Fluorescent Tracking Powder On Oxygen Consumption In Salamanders Using Either Cutaneous Or Bimodal Respiration, Sarah A. Orlofske, Kristine L. Grayson, William A. Hopkins

Biology Faculty Publications

Fluorescent powder is gaining attention as an effective method for tracking terrestrial amphibian movements, particularly for species that are too small for conventional tracking equipment. The technique requires coating portions of an animal with fluorescent powder, releasing the animal, and following the trail of powder as it is progressively lost during movement. Recent studies have shown that fluorescent powder has no negative effects on survival or growth. However, a substance that coats the skin, a major respiratory organ in most amphibians, may have sublethal effects on performance and consequently behavior. We tested the effect of fluorescent powder application on the …

Mnte-2-Pyp And Radiation In A Prostate Cancer Model: Implications For Radiotherapy, Adeola Y. Makinde

Loma Linda University Electronic Theses, Dissertations & Projects

A major limitation of successful radiation therapy in cancer treatment is the increase in normal tissue damage as higher doses are used to achieve greater tumor destruction. Radiation dose optimization in cancer therapy requires achieving maximum tumor destruction with minimal damage to normal tissue Antioxidants have been shown to protect normal tissues against radiation damage, as radiation-induced tissue damage results predominantly from reactive oxygen species that directly damage cellular components. However, for effective use as normal tissue radioprotectants in radiotherapy, these antioxidants must not protect the tumors. Mn (III) tetrakis (N-ethylpyridinium-2-yl) porphyrin (MnTE-2-PyP) is a metalloporphyrin antioxidant that has been …

Research Choice And Finance In University Bioscience, David E. Ervin, Steven T. Buccola, Hui Yang

Economics Faculty Publications and Presentations

Academic bioscience's rising importance for downstream technology and growing private sector relationships have evoked substantial policy attention. We contribute to the scrutiny by asking how university bioscientists design and finance their research, with particular attention to the mutuality of research portfolio choice and funding success. The analysis requires consideration of other major influences on academic science, including scientific norms, human capital, and institutional environment. Drawing on a national survey of university bioscientists, we find that public financial support encourages more basic investigation and private support encourages more applied investigation. Yet downstream research is only moderately more excludable than upstream. Once …

Role Of No In The Regulation Of Cortisol Synthesis In Long-Term Hypoxic Sheep Fetal Adrenal, Tshepo Rothi Monau

Loma Linda University Electronic Theses, Dissertations & Projects

Our laboratory has demonstrated that sheep fetuses adapt to long-term hypoxia (LTH) by maintaining normal level of basal plasma cortisol despite increased adrenocorticotropic hormone (ACTH). But the mechanisms involved are not fully understood. Studies have shown that nitric oxide (NO) inhibits synthesis of steroid hormones in a variety of tissues, and in different animal species. Studies also showed that hypoxia increases both expression and activity levels of the nitric oxide (NO) generating enzyme, nitric oxide synthase (NOS), in certain tissues. We have here investigated the expression level and localization of NOS isoforms in sheep fetal adrenals. We also investigated the …

Hepatitis B Virus Subgenotypes D1 And D3 Are Prevalent In Pakistan, Anwar Ali Siddiqui, Saeeda Baig, Runu Chakravarty Runu Chakravarty, Tariq Moatter

Department of Biological & Biomedical Sciences

Background:

As the hepatitis B genotyping is important for assessing its clinical implications and geographical distribution, the sub-genotypes have been found useful for determination of specific genomic markers related to hepatocarcinogenesis. In Pakistan, there is no reported data on molecular evolutionary analysis of HBV. A study was, therefore, much needed to evaluate the spectra of mutations present in the strains prevalent here.

Findings:

To confirm specificity of PCR typing, phylogenetic analysis of the pre-S1 region and the divergence was studied through 13 sequences of 362 bp (accession number EF432765 – EF432777). A total of 315 serum samples, selected from HBsAg …

Personal Reflections On Russell And Burch, Frame, And The Hsus, Martin Stephens

Animal Welfare Collection

The coincidence of anniversaries associated with the publication of William Russell and Rex Burch’s The Principles of Humane Experimental Technique, the founding of the Fund for the Replacement of Animals in Medical Experiments (FRAME), and the establishment of the collaboration between FRAME and the University of Nottingham, provides an opportunity to reflect on Russell and Burch’s legacy and how it was carried forward by FRAME. The Principles, published in 1959, was the pioneering work in what later became the alternatives or Three Rs field of replacement, reduction, and refinement of animal use. Such was the book’s initial and undeserved obscurity, …

Food, Fibre And Flowers, Fuelling The Economy With Innovation And Science, Department Of Agriculture And Food, Western Australia

Horticulture published reports

The Department of Agriculture and Food is a world leader in the rapidly growing field of agri-food and fibre research and development, providing significant benefits for Westen Australian producers, the food industry sector and consumers.

The development of value-added products through innovation and science, which deliver real opportunities for the State's agri-food export capacity, is also important.

Seven examples of the Department's science and innovation.

Horseshoe Crab Spawning Survey Protocol, Jennifer Mattei

Biology Faculty Publications

The protocol lists supplies and clothing needed for the survey, as well as which data is to be collected and when and how it should be entered for the census of horseshoe crabs on the Recapture Data Sheet for 2009. Adopted from Cape Cod and Delaware Bay survey protocols.