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Laboratory and Basic Science Research Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
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- 3-Nitrotyrosine; Early diagnosis; Electrical emission; Fast methods; Hepatic encephalopathies; Liver cirrhosis; Luminescence intensity; Photo probes; SIMPLE method; Spectrofluorimetric methods (1)
- Antidiabetic drugs; Empagliflozin; Matrix effect; Metformin; Pharmacokinetic; UPLC–MS/MS (1)
- Ashwagandha; chemical analysis; liquid chromatography; spectroscopy; Withania somnifera (1)
- Atenolol; fluorescence; green; hydrochlorothiazide; response surface methodology (1)
- Biosensor; Cardiac biomarker; Europium Complex; Luminescence; Troponin (1)
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- Chemosensor; Fluorescence intensity; Real water; UO22+ (1)
- Conducting polymer; Date-rape drugs; Flunitrazepam; Miniaturized sensor; Potentiometry (1)
- Cost benefit analysis; Cost effectiveness; Diagnosis; Organic acids; Organic solvents (1)
- Cost benefit analysis; Diagnosis; Luminescence; Organic solvents; Polyethylene glycols (1)
- Derivative ratio Transformation; Dual Amplitude Difference; Fingerprint; Green assessment; Parent spectrum restoration technique; White analytical chemistry (1)
- Drug products; Liquid chromatography; Mass spectrometry; Proteins (1)
- Glassy carbon electrodes; Hydrophobic polymers; Midazolam; Perfluorinated polyaniline; Solid-contact ion-selective electrodes (1)
- HKUST1/Zeolite A4 (1)
- Heavy metal adsorption (1)
- Tannic acid (1)
- Wastewater remediation (1)
- Publication
Articles 1 - 15 of 15
Full-Text Articles in Laboratory and Basic Science Research
Design And Functionalization Of A Hierarchical Cu-Btc/Zeolite A4 Composite With Tannic Acid For High-Performance Heavy Metal Removal: Synergistic Adsorption Mechanisms, Kinetic Insights, And Reusability Evaluation, Samar A. Salim
Nanotechnology Research Centre
This study presents the development of a novel multifunctional adsorbent Cu-BTC (HKUST-1)/Zeolite A4 composite functionalized with tannic acid—for the efficient removal of toxic heavy metal ions (Pb²⁺, Cd²⁺, Cr(VI)) from aqueous solutions. The composite was synthesized via a solvothermal process and systematically characterized by XRD, FTIR, SEM-EDS, and BET surface area analysis. The resulting material exhibited a high specific surface area (1050 m²/g), a hierarchical pore structure, and a homogeneous distribution of active sites. Batch adsorption experiments revealed superior adsorption capacities of 220 mg/g for Pb²⁺, 180 mg/g for Cd²⁺, and 150 mg/g for Cr(VI) at pH 6, surpassing those …
Fabrication Of Novel Electropolymerized Conductive Polymer Of Hydrophobic Perfluorinated Aniline As Transducer Layer On Glassy Carbon Electrode: Application To Midazolam As A Model Drug Of Benzodiazepines, Ekram Hany Mohamed, Amr Mahmoud,, , Nancy Nashat, Sally El-Mosallamy
Fabrication Of Novel Electropolymerized Conductive Polymer Of Hydrophobic Perfluorinated Aniline As Transducer Layer On Glassy Carbon Electrode: Application To Midazolam As A Model Drug Of Benzodiazepines, Ekram Hany Mohamed, Amr Mahmoud,, , Nancy Nashat, Sally El-Mosallamy
Pharmacy
The objective of this study is to fabricate solid-contact ion selective electrodes (SC-ISEs) that have long term stable potential. Various conducting polymers such as polyaniline and its derivatives have been successfully employed to improve the potential stability in SC-ISEs. Recently, the role of hydrophobicity at the interface between the conducting polymer solid contact and the ion sensing membrane has been investigated and figured out that the hydrophobic interfaces preclude water layer formation that deteriorate the SC-ISEs potential stability and reproducibility. In this work, a hydrophobic polyaniline derivative was fabricated on the surface of a glassy carbon electrode by electropolymerization of …
Development And Validation Of Molnupiravir Assessment In Bulk Powder And Pharmaceutical Formulation By The Rp-Hplc-Uv Method, Ekram Hany Mohamed, A. H. Abdel Aziz, Noha M. El Zahar,, Nour El-Din A. Abdel-Sattar, Safwat A. Mahmoud,, Mohamed S. Attia,
Development And Validation Of Molnupiravir Assessment In Bulk Powder And Pharmaceutical Formulation By The Rp-Hplc-Uv Method, Ekram Hany Mohamed, A. H. Abdel Aziz, Noha M. El Zahar,, Nour El-Din A. Abdel-Sattar, Safwat A. Mahmoud,, Mohamed S. Attia,
Pharmacy
An accurate, sensitive and selective RP-HPLC-UV method has been established for the estimation of Molnupiravir (MOL) in pure bulk powder and pharmaceutical formulation. Separation was achieved on an Inertsil C18 column (150.0 mm × 4.6 mm, 5.0 μm), using a mobile phase of 20 mM phosphate buffer pH 2.5 : acetonitrile (80 : 20, v/v%) in isocratic mode with a flow rate of 1.0 mL min−1. The λmax of MOL prepared in the chosen diluent (ethanol : water in equal proportions) was found to be 230.0 nm. The constructed calibration curve was found to be linear in the concentration range …
A Highly Selective Optical Sensor Eu-Binam For Assessment Of High Sensitivity Cardiac Troponin Tumor Marker In Serum Of Cancer Patients, Ekram Hany Mohamed, Mohamed S. Attia, Ahmed O. Youssef, Shahenda S. Ahmed
A Highly Selective Optical Sensor Eu-Binam For Assessment Of High Sensitivity Cardiac Troponin Tumor Marker In Serum Of Cancer Patients, Ekram Hany Mohamed, Mohamed S. Attia, Ahmed O. Youssef, Shahenda S. Ahmed
Pharmacy
A novel, easy, touchy and selective spectrofluorimetric technique has been successfully applied for sensitive determination of High Sensitivity Cardiac Troponin (TNHS I) in the serum samples of patients suffering malignant tumors through the usage of optical sensor Eu3+-BINAM complex. The technique is primarily based on quenching of the Eu3+-BINAM complex's luminescence intensity upon introducing various concentrations of High Sensitivity Cardiac Troponin (TNHS I). The synthesis and characterization of the optical sensor was performed via absorption and emission. The sensor was also adapted to offer excitation at 394 nm in acetonitrile at pH 7.5. Concentration of High Sensitivity Cardiac Troponin (TNHS …
Environmentally Sustainable Computationally Spectrophotometric Resolution Strategy For Analysis Single-Tablet Regimens Of Antihypertension With Overlapped Spectra, Ekram Hany Mohamed, Hayam M. Lotfy, Reem H. Obaydo,
Environmentally Sustainable Computationally Spectrophotometric Resolution Strategy For Analysis Single-Tablet Regimens Of Antihypertension With Overlapped Spectra, Ekram Hany Mohamed, Hayam M. Lotfy, Reem H. Obaydo,
Pharmacy
Single tablet regimens (STRs) for hypertension suppression improve patient satisfaction, quality of life, medication and adherence compared to multi-tablet regimens (MTRs). Development of simple cost effective and at the same time green an ecofriendly method for analysis and resolution of multicomponent and complex mixtures is an increasingly important issue in the field of pharmaceutical industry in general and analytical chemistry in specific. Thus, the presented research deals with two main goals, the first one is concerned with developing and applying a fully validated resolution plan using numerical data of signals on manipulated spectra for successive extraction of parent spectra of …
Major Biochemical Constituents Of Withania Somnifera (Ashwagandha) Extract: A Review Of Chemical Analysis, Ekram Hany Mohamed, Mahmoud Tareq Abdelwahed,, Maha A. Hegazy
Major Biochemical Constituents Of Withania Somnifera (Ashwagandha) Extract: A Review Of Chemical Analysis, Ekram Hany Mohamed, Mahmoud Tareq Abdelwahed,, Maha A. Hegazy
Pharmacy
Ashwagandha (Withania somnifera) is a very popular medicinal herb originated mainly in India and found in the Middle East and parts of Africa. Ashwagandha has gained recognition as the most revered plant in Ayurvedic medicine. Studies indicated that it is used as anxiolytic, anti-inflammatory, antioxidant, adaptogen, memory enhancer, and possess an effect on neurodegenerative diseases. Additionally, it was revealed that the plant exerts antibacterial, antiviral, antitumor, aphrodisiac, and cardiovascular protection activities. Due to the biological and nutritional importance of nutraceuticals, particularly ashwagandha, and as part of the ongoing research of the natural products and its bioactive phytochemicals, this review aims …
Development Of A Multivariate Model With Desirability-Based Optimization For Determination Of Atenolol And Hydrochlorothiazide By Eco-Friendly Hplc Method With Fluorescence Detection, Ekram Hany Mohamed, Ahmed Hemdan,, Naser F. Al-Tannak,
Development Of A Multivariate Model With Desirability-Based Optimization For Determination Of Atenolol And Hydrochlorothiazide By Eco-Friendly Hplc Method With Fluorescence Detection, Ekram Hany Mohamed, Ahmed Hemdan,, Naser F. Al-Tannak,
Pharmacy
Determination of a widely used antihypertensive combination of atenolol and hydrochlorothiazide was achieved by rapid and eco-friendly high-performance liquid chromatography method combined with fluorescence detection. The response surface methodology is conducive to the complete separation of the two drugs in a shorter analysis time. The separation of the mixture was achieved using an Inertsil C18 analytical column (150 × 4.6 mm, 5 µ). The mobile phase used was ethanol: potassium dihydrogen phosphate at pH 3 (65:35 v/v) and the flow rate was 0.7 mL/min. The fluorescence detector operated at excitation and emission wavelengths of 230 and 310 nm (atenolol) and …
A Highly Selective And Sensitive Spectrofluorimetric Method For The Assessment Of 3-Nitrotyrosine In Serum Using (Eu(Tta)3phen) Photo Probe, Ekram Hany Mohamed, Mohamed S. S. Attia, Ahmed O. Youssef, Nour El-Din A. Abdel-Sattar, Mohammed A. Amin,, Sarah Alharthi, Safwat A. Mahmoud,, Mona N. Abou-Omar,
A Highly Selective And Sensitive Spectrofluorimetric Method For The Assessment Of 3-Nitrotyrosine In Serum Using (Eu(Tta)3phen) Photo Probe, Ekram Hany Mohamed, Mohamed S. S. Attia, Ahmed O. Youssef, Nour El-Din A. Abdel-Sattar, Mohammed A. Amin,, Sarah Alharthi, Safwat A. Mahmoud,, Mona N. Abou-Omar,
Pharmacy
A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)3Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)3Phen) complex in DMSO at pH = 9 and λem = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)3Phen) complex particularly the electrical emission band at λem = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis
A Novel Cyanopyridine Derived Fluorescent Sensor For Selective Determination Of Uranyl Ions In Different Water Samples, Ekram Hany Mohamed, Amany R Salem, Abeer M. El-Naggar,, Mohammed A. Amin,, Mohamed S. S. Attia
A Novel Cyanopyridine Derived Fluorescent Sensor For Selective Determination Of Uranyl Ions In Different Water Samples, Ekram Hany Mohamed, Amany R Salem, Abeer M. El-Naggar,, Mohammed A. Amin,, Mohamed S. S. Attia
Pharmacy
A newly developed spectrofluorometric method is based on the fabrication of an optical cyanopyridine derived sensor, 4-(6-diaryl)-2-oxo -1,2-dihydropyridine-3-carbonitrile using microwave irradiation. The fluorescence intensity of the proposed chemo sensor was obviously quenched in DMSO owing to the presence of UO22+ ions at λex/λem = 320/360 nm. The quenching effect of the cyano-derivative based sensor was found to be proportional to the concentration of UO22+ ion ranging between 9.60 × 10–9–5.28 × 10–5 mol L−1 with a detection and quantification limits of 1.71 × 10–11 and 5.2 × 10–11 mol L−1, respectively. © 2021, Akadémiai Kiadó, Budapest, Hungary.
Tb3+-Atorvastatin Doped In Poly(Ethylene Glycol) Optical Biosensor For Selective Determination Of Progesterone And Testosterone In Serum Samples, Ekram Hany Mohamed, Mohamed S. S. Attia, Safwat A. Mahmoud,, Amal M. Ahmed,, Tarek A. Amin, Ahmed O. Youssef, Mona N. Abou-Omar,, Mohammed A. C Amin,
Tb3+-Atorvastatin Doped In Poly(Ethylene Glycol) Optical Biosensor For Selective Determination Of Progesterone And Testosterone In Serum Samples, Ekram Hany Mohamed, Mohamed S. S. Attia, Safwat A. Mahmoud,, Amal M. Ahmed,, Tarek A. Amin, Ahmed O. Youssef, Mona N. Abou-Omar,, Mohammed A. C Amin,
Pharmacy
An innovative, simple and cost effective Tb3+-atorvastatin photo probe was designed and used as a core for a spectrofluorometric approach to sensitively determine two vital biological compounds in serum samples. Tb3+-atorvastatin complex displays a characteristic electrical band with λem at 545 nm with significant luminescence intensity, which is quenched in the presence of progesterone and testosterone at two variant sets of pH; 6.2 and 7.5, respectively. The conditions were optimized and the best solvent for operation was found to be acetonitrile with λex at 320 nm. Progesterone and testosterone were assessed in serum samples using the same optimal conditions within …
New Tb3+-Simvastatin Optical Biosensor For Sensitive Determination Of Folic Acid, Progesterone, Testosterone And Vitamin D3in Biological Fluids, Ekram Hany Mohamed, Mohamed S. S. Attia, Ahmed O. Youssef, Mona N. Abou-Omar,, Amal M. Ahmed,, Safwat A. Mahmoud,, Mohammed A. Amin, Tarek A. Mahmoud,
New Tb3+-Simvastatin Optical Biosensor For Sensitive Determination Of Folic Acid, Progesterone, Testosterone And Vitamin D3in Biological Fluids, Ekram Hany Mohamed, Mohamed S. S. Attia, Ahmed O. Youssef, Mona N. Abou-Omar,, Amal M. Ahmed,, Safwat A. Mahmoud,, Mohammed A. Amin, Tarek A. Mahmoud,
Pharmacy
An innovative, simple and cost effective Tb3+-simvastatin photo probe was designed and used as a core for a spectrofluorometric approach to sensitively determine four vital biological compounds in different matrices. A Tb3+-simvastatin complex displays a characteristic electrical band with λem at 545 nm with significant luminescence intensity, which is quenched in the presence of folic acid, progesterone, testosterone and vitamin D3 at four variant sets of pH: 5.0, 6.2, 7.5 and 9.0, respectively. The conditions were optimized and the best solvent for operation was found to be acetonitrile at λex at 340 nm. Folic acid was successfully estimated in tablet …
Simultaneous Determination Of Avanafil And Dapoxetine In Human Plasma Using Liquid Chromatography/Tandem Mass Spectrometry (Lc-Ms/Ms) Based On A Protein Precipitation Technique, Ekram Hany Mohamed, Mohamed S. S. Attia, Mona N. Abou-Omar,, Abdelaziz M Annadi,, Ahmed O. Youssef, Mohamed A. Amin, Noha M. El Zahar,
Simultaneous Determination Of Avanafil And Dapoxetine In Human Plasma Using Liquid Chromatography/Tandem Mass Spectrometry (Lc-Ms/Ms) Based On A Protein Precipitation Technique, Ekram Hany Mohamed, Mohamed S. S. Attia, Mona N. Abou-Omar,, Abdelaziz M Annadi,, Ahmed O. Youssef, Mohamed A. Amin, Noha M. El Zahar,
Pharmacy
A rapid and selective LC-MS/MS method is described for the simultaneous assay of Avanafil and Dapoxetine in human plasmaviaa protein precipitation (PP) sample preparation technique. Tadalafil was chosen as the internal standard reaching good recovery and reproducibility while diminishing the effects of the matrix. An Agilent Zorbax Eclipse XDB C18column (4.6 × 50 mm, 1.8 μm) was used for the chromatographic separation and analysis, while 0.1% formic acid : acetonitrile (60 : 40, v/v) was utilized at a flow rate of 0.5 mL min−1. It was revealed that 6 min stop time accomplished the best separation. The assay was linear …
Novel Optical Biosensor Based On A Nano-Gold Coated By Schiff Base Doped In Sol/Gel Matrix For Sensitive Screening Of Oncomarker Ca-125, Ekram Hany Mohamed, Mohamed S. S. Attia, Mona N. Abou-Omar,, Mohammed A. Amin,, Rabah Boukherroub, Hisham Afify
Novel Optical Biosensor Based On A Nano-Gold Coated By Schiff Base Doped In Sol/Gel Matrix For Sensitive Screening Of Oncomarker Ca-125, Ekram Hany Mohamed, Mohamed S. S. Attia, Mona N. Abou-Omar,, Mohammed A. Amin,, Rabah Boukherroub, Hisham Afify
Pharmacy
The urge for sensitive, facile, minimally invasive, and fast detection method of CA-125, a significant and crucial biomarker in ovarian malignancy, is currently substantial. This paper describes the detailed construction and characterization of a newly designed optical nano-biosensor to detect CA-125 accurately and sensitively. The fabricated sensor consists of a nano-gold thin film doped into a matrix of sol-gel, exhibiting a centered fluorescence band at 423 nm when excited at 340 nm. The quantification of CA-125 relies on its quenching ability of this fluorescence signal. The sensor was challenged to evaluate its sensitivity and specificity in detecting CA-125 present in …
All Solid-State Miniaturized Potentiometric Sensors For Flunitrazepam Determination In Beverages, Ekram Hany Mohamed, Mahmoud Tantawy, Ali Yahia
All Solid-State Miniaturized Potentiometric Sensors For Flunitrazepam Determination In Beverages, Ekram Hany Mohamed, Mahmoud Tantawy, Ali Yahia
Pharmacy
Flunitrazepam is one of the frequently used hypnotic drugs to incapacitate victims for sexual assault. Appropriate diagnostic tools should be available to victims regarding the growing concern about “date-rape drugs” and their adverse impact on society. Miniaturized screen-printed potentiometric sensors offer crucial point-of-care devices that alleviate this serious problem. In this study, all solid-state screen-printed potentiometric flunitrazepam sensors have been designed. The paper device was printed with silver and carbon ink. Formation of an aqueous layer in the interface between carbon-conducting material and ion-sensing membrane nevertheless poses low reproducibility in the solid-contact electrodes. Accordingly, poly(3,4-ethylenedioxythiophene) (PEDT) nano-dispersion was applied as …
Validation Of A Novel Uplc-Ms/Ms Method For Estimation Of Metformin And Empagliflozin Simultaneously In Human Plasma Using Freezing Lipid Precipitation Approach And Its Application To Pharmacokinetic Study, Ekram Hany Mohamed, Mona N. Abou-Omar,, Mohamed S. S. Attia, Ahmed O. Youssef, Sarah Alharthi,, M. Kenawy
Validation Of A Novel Uplc-Ms/Ms Method For Estimation Of Metformin And Empagliflozin Simultaneously In Human Plasma Using Freezing Lipid Precipitation Approach And Its Application To Pharmacokinetic Study, Ekram Hany Mohamed, Mona N. Abou-Omar,, Mohamed S. S. Attia, Ahmed O. Youssef, Sarah Alharthi,, M. Kenawy
Pharmacy
fast, sensitive one step UPLC ESI-MS/MS method was successfully applied for the simultaneous estimation of two concurrently administrated antidiabetic drugs, Metformin (MET) and Empagliflozin (EMPA) in human plasma. Metformin-d6 (MET-d6) and Empagliflozin-d4 (EMPA-d4) were utilized as internal standards. Extraction of the analytes from the human plasma was performed through acetonitrile precipitation technique followed by freezing the precipitated plasma proteins and lipids to minimize the matrix effect. Chromatographic analysis was developed on Acquity UPLC BEH C18 column (1.7 μm, 2.1 × 50 mm) using isocratic elution mode. A mobile phase of formic acid (0.01 %): acetonitrile (70:30 v/v) with a flow …