Laboratory and Basic Science Research Commons^™

Fish Tissue Digestion And Microplastic Polymer Identification Protocol Using Open-Source Ftir Database, Shawn L. Kissinger, Vick-Ariel Privert, Jason Bystriansky, Kyle A. Grice

DePaul Discoveries

Microplastic (MP) contamination in aquatic ecosystems poses significant concerns for environmental and public health, as seafood represents a critical food source worldwide. Despite increasing evidence of MP presence in marine organisms, efficient and accessible detection methods remain essential for characterizing contamination patterns and informing regulatory frameworks. This study extracted MPs from brain, gill, intestine, liver, and muscle tissues of gilt-head bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), two commercially important species from the Gulf of Cádiz, Spain, using a NaOH–HNO₃ chemical digestion protocol. Isolated particles were characterized by Fourier Transform Infrared (FTIR) spectroscopy, with …

The Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier

Poster Presentations

Music is a universal component of human culture that has been demonstrated influencing emotional, cognitive, and physiological responses, specifically heart rate and hormonal responses (Mehr et al., 2019; Trappe, 2012). Recently, this research has expended to explore how music may affect non-human animals, particularly dogs, due to their roles as therapy animals, service animals, and human companions (Lindig et al., 2020; Gee et al., 2021). Being able to understand how music influences canine stress and relaxation hold important applications in environments such as shelters, veterinary clinics, and at home. One important feature of music is tempo, or beats per minute …

Assessing Urban–Rural Differences In Largemouth Bass Abundance In Minnesota, Jonathan Gates

Journal of Earth and Life Science

Urbanization can significantly alter freshwater ecosystems and influence sport fish populations. This study examined differences in largemouth bass Micropterus nigricans catch-per-unit-effort (CPUE) between urban and rural lakes in Minnesota and evaluated temporal changes between 2000–2010 and 2011–2021. Forty lakes (20 urban, 20 rural) were selected using Minnesota Department of Natural Resources fisheries survey data. Lakes were classified using NLCD 2019 land cover data, with ≥10% developed land designated as urban. Nonparametric analyses (Wilcoxon rank-sum and signed-rank tests) were used to assess spatial and temporal differences in CPUE. Results showed that rural lakes had consistently higher median CPUE than urban lakes …

Neonicotinoid Bioaccumulation In Bluegill And Bullhead Within Lake Julia And Lake Zumwalde, Jake Stuber

Journal of Earth and Life Science

Brown Bullhead Ameiurus nebulosus, Yellow Bullhead Ameiurus natalis, and Bluegill Lepomis macrochirus are unique in their stark differences. Bullhead are scaleless bottom feeders that can tolerate high pollution and low O₂ conditions. In contrast, Bluegill are scaled, aggressive nesters and are less tolerant of high pollutants or low-oxygen conditions. Among these pollutants include a class of chemicals called neonicotinoids (hereafter referred to as neonics). Neonics are a class of insecticides primarily used within agriculture and pest management. Due to the effectiveness, application has escalated on a global scale. They have been well documented by many toxicology assessments. …

The Chemistry Of Red Lipstick: The Impact Red Lipstick Has On Society, Lilliana Weldeslassie

Electronic Theses & Dissertations (2024 - present)

Cosmetic chemistry is deeply embedded in everyday life, shaping the products people use from morning to night. Among these, red lipstick stands out as a formulation that blends complex chemistry with a cultural meaning. This thesis examines the scientific foundations of red lipstick through its chemical composition, pigment structure, toxicological history, analytical methods, and evolving sustainability practices. Historically, red lipsticks relied on high-risk ingredients such as lead, mercury, and other toxic metals, reflecting an era before chemical safety and regulatory oversight were incorporated.²¹ Modern formulations have established safer synthetic dyes, natural waxes, plant-based oils, and stabilizers created to optimize …

Two Major Ontario Hospital Labs Bought Research Dogs From U.S. Breeder With Troubled Pastdogs Bred For Scientific And Medical Testing Suffer Abuse As The Scientific Industry Increasingly Turns To Alternatives For Research., Allison Hunter, Mary Beech, Sami Marshak, Kate Morano

Capstones

The purpose-bred dog breeding industry for research is little known. Each year, thousands of dogs are bred for scientific and medical testing, supplied to research facilities by a handful of major commercial breeders. But gaps in enforcement and regulation have allowed abuses to persist, even as the industry increasingly turns to alternatives to animal testing. Animal rights advocates have been decrying the treatment of these dogs. A federal Inspector General report found lax oversight of the industry, raising further concerns about how dogs are treated. This story was reported in collaboration with the Investigative Journalism Bureau, based in Toronto, and …

The Science Of Sound: Studying The Cognitive, Emotional, And Physiological Effects Of Frequency, Genre, And Music, Jett Yarborough

Senior Honors Theses

Music influences emotion, physiology, and cognition, yet little is known about how the frequency it is tuned to affects these influences. Prior research has shown that music tuned to 432 Hz can reduce stress, lower blood pressure, and improve sleep. My colleagues and I conducted two studies to investigate this. Our research found that music tuned to 432 Hz promotes a significant increase in memory retention and induces a state of focus and relaxation. These results suggest that shifting from the standard tuning frequency of 440 Hz to 432 Hz could have a profoundly positive impact on our daily lives. …

Enhancing Cataract Surgery Outcomes: Optimal Use Of Pre- And Post-Operative Eye Drops, Keith Skolnick M.D., Anu Valiaveedu

Mako: NSU Undergraduate Student Journal

Many preoperative and postoperative cataract patients struggle with comprehending the use of prescription medication as directed. Language barriers and low health literacy levels are major factors contributing to improper use of prescriptions. To increase patients comprehension, the Fort Lauderdale Eye Institute employed an educational intervention consisting of a live presentation and an instructional video. Results found that 44% of patients were hesitant to ask questions to clinical staff, 32% felt overwhelmed, and nearly 70% lacked confidence in using their prescribed eye drops. Following the intervention, 91% of patients reported increased confidence in their medications, and most indicated that the video …

Scientist Perspectives: A Correlation Analysis Of Translational Science Competencies Compared With Principles Of Engagement And Knowledge Integration, Rachel Wenzl

Theses & Dissertations

The competencies identified in the development of the “Fundamental Characteristics of the Translational Scientist” may not consider and fully integrate the concepts of knowledge integration and engagement, key components of the translational science spectrum. This gap may be widening and deepening the “valley of death” and exacerbating ongoing challenges in the field of translational science. This dissertation explored the association of knowledge integration, stakeholder engagement, and community engagement principles compared with translational science competencies.

Utilizing content review and a non-experimental, correlation study design, data were collected through a quantitative survey from adult research subjects all self-identified as a research investigator …

Investigating The Relationship Among Environmental Factors, Reproductive Status, And Stress In A Preclinical Model Of Endocrine Function In Women’S Translational Health, Bianca A. Camacho, Laura Emma Garcia, Raquel Villarreal, Mario Gil

Research Symposium

Background: The average menstrual cycle of a healthy adult woman is a duration of approximately 28 days, during which in the follicular phase (first 14 days) the progesterone hormone is at a stable level while estrogen increases to its peak level on day 14. Day 14 is known as ovulation, during which progesterone increases during its Luteinizing Hormone (LH) surge while estrogen decreases. Menses, known colloquially as a woman’s “period,” begins approximately at day 14 after ovulation and lasts an average of 7 days. The luteal phase, otherwise known as the secretory phase, lasts for the remaining 14 days until …

An Approach To Estimate Angling Effort, Catch, And Harvest Of Burbot Lota Lota, Collin J. Mitchell

Biology Graduate Theses

Once commonly thought of as a nuisance, Burbot Lota lota are now being frequently targeted by anglers in Minnesota. However, the amount of angling effort directed at Burbot, the distribution of effort, catch, and harvest are unknown. Historically, creel surveys have been used to estimate angling effort, catch, and harvest. Additionally, digital cameras have been used independently and supplementally to estimate angling effort, catch, and harvest. In this study, we estimate angling effort, catch, and harvest of Burbot using creel survey techniques and motion-activated digital cameras on Bad Medicine Lake, Minnesota, while also investigating and exploring the limitations of these …

Modeling Wild Rice Bed Health: A Dual Metric Approach For Bed Strength And Invasive Impact, Seth Sisneros-Martinez

Journal of Earth and Life Science

Wild rice (mannoomin; Zizania palustris) is an aquatic grain that grows in slow-moving rivers and shallow bays throughout northern Minnesota. In Headquarters Bay on Leech Lake, wild rice has served as a cultural staple for Indigenous communities for centuries. However, its population has declined in recent years due to multiple environmental issues. One potential contributor is Eurasian watermilfoil (Myriophyllum spicatum; EWM), an invasive species that forms dense mats capable of outcompeting native aquatic vegetation, including wild rice. EWM was first recorded on the southern shoreline of Leech Lake in 2005 and has since become widespread. This study …

The Effect Of Zebra Mussels On Minnesota Fish Communities, Benjamin D. Strei

Journal of Earth and Life Science

Zebra mussels Dreissena polymorpha are an aquatic invasive species that has been spreading quickly throughout Minnesota water bodies since 1989. Zebra mussels are known to change certain morphological conditions in lakes such as turbidity, phytoplankton abundance, and aquatic invertebrate populations. However, studies showing impacts of the infestation of zebra mussels on the health of a fish community have been hard to prove due to a lack of repeated surveys and a suite of other confounding variables such as natural and seasonal variability, plant community, and lake morphometry. Therefore, the objective of this study is to determine whether zebra mussels have …

High Fat Diet & Social Isolation: Interactive Effects On Pain, Cognition, & Neuroinflammation, Ian M. Campuzano

Research Psychology Theses

Prior research has established a role for both social isolation and exposure to high fat Western diets in altering a range of behaviors from reduced memory performance to increased depression-like behaviors. The present study scrutinizes the interplay among these variables during the peri-adolescent developmental phase, utilizing Long-Evans rats as the experimental model. Our overarching hypothesis is that rats exposed to either social isolation, a high-fat diet, or both will result in heightened pain sensitivity, diminished cognitive flexibility, and increased neuroinflammatory responses within brain regions implicated in sociability, cognition, memory, and pain processing. Behavioral flexibility will be assessed using a maze-based …

Microplastic Accumulation In The Marsh Periwinkle (Littoraria Irrorata), Sarah Keaton, Hannah Lukhard, Eli Windy, Manar Hasan

DePaul Discoveries

Contamination of oceans by microplastics (mm) currently poses a major threat to aquatic and terrestrial ecosystems worldwide. Recent attention towards this issue has raised questions about the extent to which microplastics have accumulated in the environment, and has led to an increase in studies on the effects of microplastics in various organisms. However, levels of contamination in protected natural areas are still largely unexplored, yet can offer an important empirical perspective on the issue. In addition, little is known about the potential effects of microplastics on behavior in the field. This research was conducted within the protected ACE Basin National …

The Biobehavioral Effects Of Embryonic Exposure To Neural Inflammation And Oxidative Stress In Zebrafish, Dalton J. Anderson

Honors College Theses

The purpose of this research is to improve understanding of the neurodevelopmental effects of embryonic exposure to elevated inflammation and oxidative stress induced by the antipyretic drug acetaminophen (APAP). Our study was the first to examine the interactive effects of APAP and inflammation in zebrafish embryos and how the treatments affect brain development and larval behavior. Experimental groups of zebrafish larvae were exposed to lipopolysaccharide (LPS) to induce inflammation, APAP, or LPS + APAP and larval behavior was analyzed using Ethovision automated behavioral tracking software. We also measured changes in whole-brain Glycogen Synthase Kinase 3 Beta (GSK3B) and GSK3B phosphorylation, …

Mild Traumatic Brain Injury In Yucatan Minipigs: Assessing Changes In Approachability And Mechanical Nociception Following Diffuse Central Fluid Percussion Injury, Mark D. Pavlichenko

Theses and Dissertations

Traumatic brain injury (TBI) is a major healthcare concern affecting millions. Symptoms of TBI include sensory sensitivity and social changes that may persist long-term. Due to similarities in cytoarchitecture, metabolism, and inflammation, higher order minipig models are advantageous for translational TBI research, however, there remains a shortage of information regarding their behavioral and sensory sequelae following injury. Therefore, in this study, we assessed changes in sociability and somatosensation in adult male and female Yucatan minipigs for up to one-week following a sham or central fluid percussion injury (cFPI). Specifically, the voluntary human approach task (VHAT) and the forced human approach …

Exploration Of The Implementation Of An Integrated Electronic Laboratory Information Management System On Quality Diagnostics Service Indicators At A County Level Public Hospital In Western Kenya., Kelly Allen

Capstone Collection

Underinvestment in pathology and laboratory capacity caused by low visibility in research and in prioritization by public health leaders results in limited effective healthcare coverage and an estimated 1.1 million premature deaths annually in Low-and-Middle-Income Countries. Kenya’s public health laboratories provide a median 41% of the Essential Diagnostic List to their patients and in Kisumu County, as much as 44.2% of the population has little to no access to essential diagnostics. The government of Kisumu implemented the county Health Laboratory Strategic Plan 2018-2022 to address this public health challenge. Little information exists on the effectiveness of these initiatives and the …

Estimating The Minimum Number Of Individuals (Mni) For Skeletal Collections With Consideration To The Introduction Of Procurement Bias, M. Elizabeth Dyess, T. Heil

2023 Symposium

Of the competing methods for the estimation of the number of individuals represented within a skeletal assemblage, variations of the calculation of MNI (Minimum Number of Individuals) are most often employed. This presentation provides the preliminary results of an exhaustive study designed to determine the minimum number of individuals represented within a collection of 1,065 skeletal elements and fragments, belonging to the Eastern Washington University Anthropology Program. Results produced by established methods of computation were reinterpreted to account for the introduction of Procurement Bias in the calculation of MNI.

Synthesis Of Biomass-Based Graphene Nanomaterials For Aqueous Heavy Metal Removal And Cement-Based Composite Property Enhancement, Tharindu N. Karunaratne

Theses and Dissertations

Utilizing biomass such as lignin, bamboo, soybean, corn stalk, rice husk, etc., as a carbon source to produce graphene-based nanomaterials has been reported recently. However, the potential of using such nanomaterials for engineering and environmental applications has not been realized. This dissertation investigates the use of graphene-based nanomaterials synthesized from using biomass as a carbon source for water remediation and cement-based composites’ (CBCs) property enhancement.

The first chapter introduces graphene and graphene-based nanomaterials, as well as the synthesis and application of graphene-based nanomaterials for removing heavy metals in an aqueous solution and for property enhancement in CBCs. The experimental investigation …

It Turned Into A Bioblitz: Urban Data Collection For Building Scientific Literacy And Environmental Connection, Kelly O'Donnell, Lisa Brundage

Publications and Research

In 2013, Macaulay Honors College redesigned its required science curriculum to focus on scientific literacy skills rather than content. Central to this shift was inclusion of a data collection event, a BioBlitz, to provide students with the basis for their own semester-long research projects. Students are teamed with naturalists in an urban green space to find as many species as they can in 24 h and to contribute to a global biodiversity database via the app iNaturalist. We have learned two important lessons: (1) developing an interdisciplinary curriculum with a high degree of experiential learning is more successful when both …

Exploring Nitrogen Fixation In Methanosarcina Acetivorans, Rylee Cook

Biological Sciences Undergraduate Honors Theses

As of 2016, the genomes of over sixty species of methanogenic archaea have been sequenced.4 Research on the genomes of methanogens such as M. acetivorans led to the characterization of numerous genes with unknown functions. Genes similar to those of nitrogenase have been shown to possess functions unrelated to nitrogen fixation, such as F430 biosynthesis genes. 5,6,7 My research focused on the uncharacterized gene clusters ma2032-33 and ma1631-33 (Figure 1). These genes have conserved nitrogenase domains: ma2032 has fused nifH and nifD domains, while ma2033 has a nifD domain. The ma1631-33 gene cluster contains two nitrogenase homologs and one iron …

Scientific Development Of An Integrated Workflow For Latent Print, Questioned Document, And Dna Processing Of Paper Evidence, Ashley Morgan

Dissertations, Theses, and Capstone Projects

Touch paper evidence could be the source of probative human DNA but recovery is challenging and forensic laboratories instead prioritize processing by the Latent Print and Questioned Document disciplines. Recent advances in DNA collection methods and the increased sensitivity of STR typing kits have improved success rates for DNA testing of paper evidence; but prior to implementing DNA collection, laboratories have to decide in which order to examine paper for the different types of forensic evidence. This thesis developed and tested a multi-discipline workflow for processing paper evidence by DNA, Latent Prints and Questioned Documents experts. Preliminary sampling studies indicated …

Safety In The Woods: Studying The Effectiveness Of The Kentucky Master Logger Program, Austin Winn Leake

Theses and Dissertations--Community & Leadership Development

Logging is a dangerous field; in this study the researcher looks to see the knowledge both before and after professional loggers participate in the Kentucky Master Logger Program. This information was used to see the overall effectiveness of the Kentucky Master Logger Program. The researcher found an overall improvement in logging professionals’ perceptions and understanding of logging safety. The researcher recommends creating an emphasis on a safe work culture through safety incentives and a greater focus on less used personal protective equipment such as insecticide

Laboratory Research Skills (Permanent), Joanna Burkhardt

Library Impact Statements

No abstract provided.

Laboratory Research Skills, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Stage Of Estrous Cycle Influences Behavior After Social Stress, Alexander Jonah Rodriguez

Honors Thesis

Since women are almost twice as likely to develop major depression compared to men, research into this and other potentially debilitating mood disorders using behavioral studies should involve females despite hormonal, physiological, and potential behavior changes due to reproductive cyclicity. Until recently, most studies exclusively used males, thus limiting results of previous research to half of the population. Using adult female C57BL/6N mice as model organisms, we exposed them to novel forms of social stress across five days which encompassed the four-day estrous cycle to determine if the stages of the estrous cycle had an impact on behavior when they …

Corticospinal Excitability During A Perspective Taking Task As Measured By Tms-Induced Motor Evoked Potentials, Elizabeth Murray, Janet Brenya, Katherine Chavarria, Karen J. Kelly, Anjel Fierst, Nathira Ahmad, Caroline Anton, Layla Shaffer, Kairavi Kapila, Logan Driever, Kayla Weaver, Caroline Dial, Maya Crawford, Iso Hartman, Tommy Infantino, Fiona Butler, Abigail Straus, Shakeera L. Walker, Brianna Balugas, Matthew Pardillo, Briana Goncalves, Julian Keenan

Department of Biology Faculty Scholarship and Creative Works

Only by understanding the ability to take a third-person perspective can we begin to elucidate the neural processes responsible for one’s inimitable conscious experience. The current study examined differences in hemispheric laterality during a first-person perspective (1PP) and third-person perspective (3PP) taking task, using transcranial magnetic stimulation (TMS). Participants were asked to take either the 1PP or 3PP when identifying the number of spheres in a virtual scene. During this task, single-pulse TMS was delivered to the motor cortex of both the left and right hemispheres of 10 healthy volunteers. Measures of TMS-induced motor-evoked potentials (MEPs) of the contralateral abductor …

Developmental Influences On The Initial Subjective Rewarding Effects Of Etoh, Madison Waldron

Master’s Theses

The present studies were aimed to better understand developmental contributions to the risk for disordered drinking, and facilitate the long-term goal of developing effective interventions for individuals at high risk for alcohol use disorders. Experiment 1 assessed the effect of adolescent pre-exposure to ethanol on adult place preference, as well as, sex- and beta-endorphin(bE)-related contributions. Adolescent C57BL/6J and bE deficient mice were injected with 1.5g/kg of ethanol or saline and put back into their home cages. At the time of adulthood, we employed a single-exposure conditioned place preference paradigm (SE-CPP) to investigate the impact of preexposure on the initial subjective …

Effects Of Acute And Chronic Nicotine Administration On Choice Of Probabilistic Outcomes, Katya A. Nolder

Graduate Theses, Dissertations, and Problem Reports (ETD)

Risky choice can be operationally defined as the choice for a larger, uncertain reinforcer over a smaller, certain reinforcer. Research suggests smokers engage in more risky or maladaptive decisions when compared to nonsmokers. The relation between nicotine and risky choice could benefit from further investigation, since nicotine is the active substance of tobacco products that maintains tobacco addiction. Acute nicotine administration has shown to alter risky choice; however, since the everyday smoker uses nicotine repeatedly, more research on chronic administration is warranted and would allow for assessment of tolerance or sensitization of these effects. The present study investigated effects of …