Laboratory and Basic Science Research Commons^™

Investigating The Antibacterial And Immunomodulatory Properties Of Lactobacillus Acidophilus Postbiotics, Rachael M. Wilson

Graduate School of Biomedical Sciences Theses and Dissertations

Probiotics are nonpathogenic microorganisms that have been extensively studied for their ability to prevent various infectious, gastrointestinal, and autoimmune diseases. The mechanisms underlying these probiotic effects have not been elucidated. However, we and other researchers have evidence suggesting that probiotic bacteria secrete metabolites that are antimicrobial and anti-inflammatory. As such, we developed a methodology to collect the secreted metabolites from a probiotic bacterium, Lactobacillus acidophilus, and tested this cell free filtrate (CFF) both in vitro and in vivo. Using this CFF, we have demonstrated that L. acidophilus secretes a molecule(s) that has specific bactericidal activity against the opportunistic pathogen, Pseudomonas …

Med13 Degradation Defines A New Receptor-Mediated Autophagy Pathway Activated By Nutrient Deprivation, Sara E. Hanley

Graduate School of Biomedical Sciences Theses and Dissertations

Cells are exposed to an enormous amount of diverse extracellular cues but have a limited arsenal of weapons for protecting and maintaining homeostasis. To overcome these restrictions, nature has engineered proteins that have multiple functions. The pleiotropy of using one protein to carry out a variety of functions allows cells to rapidly execute tailored responses to a diverse set of signals. The Cdk8 kinase module (CKM) is a conserved detachable unit of the Mediator complex predominantly known for its role in transcriptional regulation. The CKM is composed of four proteins, the scaffolding proteins Med13 and Med12, as well as the …

Preliminary Report On Fossil Marine Vertebrates From The Mid-Pfeifer Shale (Upper Cretaceous) From Central Kansas, Alison G. Mieczkowski

2023 Midwest Ecology & Evolution Conference

The Pfeifer Shale is a 93-million-years-old rock unit belonging to the Upper Cretaceous Greenhorn Limestone broadly distributed in central Kansas, where it formed in the Western Interior Seaway, a Late Cretaceous epicontinental seaway in North America. Here, we describe fossil marine vertebrates collected from the middle portion of the Pfeifer Shale at a locality in Russell County, Kansas. Surface-collected specimens are largely represented by teeth of some extinct sharks, such as Ptychodus, Cretoxyrhina, and Squalicorax, that are typical for rock units of the Western Interior Seaway immediately above and below the Pfeifer Shale. Examination of microscopic fossils …

Eliminating Barriers To Prosthetic Use Following Lower Extremity Amputation, Matthew Laskovy, Graham Long, Samuel Osei, Jonathon Murdock, Diane Studzinski, Rose E. Callahan, Otto W. Brown

Conference Presentation Abstracts

OBJECTIVES: Approximately 60,000 lower extremity amputations (LEA) are performed annually in the United States and 38%-55% of those individuals are fitted for a prosthesis. The average price of a lower extremity prosthetic device alone is $10,000, not including the associated costs of physical therapy and prosthetic fitting and maintenance. The most important factor influencing quality of life for individuals who have undergone LEA is the ability to ambulate with a prosthesis. The goal of our study was to investigate characteristics of individuals who abandon use of their prosthesis following LEA and identify any modifiable factors that may increase long-term successful …

Accelerometry-Based Analysis Of Postural Sway In Parkinson's Disease Patients With Levodopa-Induced Dyskinesia, Chandler Brock

UNO Student Research and Creative Activity Fair

Parkinson’s disease (PD) is a progressive neurodegenerative disorder, with patient numbers projected to double to 12 million in the next 20 years. Levodopa-induced dyskinesia (LID) is a major problem associated with the long-term use of levodopa for symptomatic treatment of PD. These involuntary movements can become disabling and may interfere with quality of life. Our prior research showed that PD w/ LID were less stable while standing (i.e., increased postural sway) and had a higher incidence of falls. The aim of this study is to determine if postural sway properties are altered by LID via decomposing the sway signal. We …

Effect Of Dual Tasking And Levodopa-Induced Dyskinesia On Postural Sway In People With Parkinson's Disease, Joseph Ayotunde Aderonmu, Carolin Curtze

UNO Student Research and Creative Activity Fair

Parkinson's disease (PD) is a neurodegenerative disorder that results in motor impairments such as gait and balance deficits. Levodopa is one of the most effective drugs in treating the slowness of movement in individuals with PD. However, the long-term use of levodopa in treating PD often causes undesirable involuntary and uncontrollable movements, known as levodopa-induced dyskinesia (LID). LID is a known cause of increased postural sway. Yet, the relative contribution of the body segments often affected by dyskinesia to postural sway is unknown. We aimed to investigate the contribution of different body segments to postural sway in PD for individuals …

Rna Virus-Mediated Changes In Organismal Oxygen Consumption Rate In Young And Old Drosophila Melanogaster Males, Eli Hagedorn, Dean Bunnell, Beate Henschel, Daniel Smith Jr, Stephanie Dickinson, Andrew Brown, Maria De Luca, Ashley Turner, Stanislava Chtarbanova

Research, Publications & Creative Work

Aging is accompanied by increased susceptibility to infections including with viral pathogens resulting in higher morbidity and mortality among the elderly. Significant changes in host metabolism can take place following virus infection. Efficient immune responses are energetically costly, and viruses divert host molecular resources to promote their own replication. Virus-induced metabolic reprogramming could impact infection outcomes, however, how this is affected by aging and impacts organismal survival remains poorly understood. RNA virus infection of Drosophila melanogaster with Flock House virus (FHV) is an effective model to study antiviral responses with age, where older flies die faster than younger flies due …

Thymic Stromal Lymphopoietin Participates In The Host Response To Intra-Amniotic Inflammation Leading To Preterm Labor And Birth, Tomi Kanninen, Li Tao, Roberto Romero, Yi Xu, Marcia Arenas-Hernandez, Jose Galaz, Zhenjie Liu, Derek Miller, Dustyn Levenson, Jonathan M. Greenberg, Jonathan Panzer, Justin Padron, Kevin Theis, Nardhy Gomez-Lopez Phd

Medical Student Research Symposium

Objective: To determine if bacteria (Ureaplasma parvum and Sneathia spp.) associated with intra-amniotic infection can trigger the induction of cytokine Thymic stromal lymphopoietin (TSLP) in human amnion epithelial cells (hAECs) in vitro.

Material or subjects: Amniotic fluid and chorioamniotic membrane (CAM) were collected from women with sPTL who delivered at term (n=30) or preterm without intra-amniotic inflammation (n=34), with sterile intra-amniotic inflammation (SIAI, n=27), or with intra-amniotic infection (IAI, n=17). Amnion epithelial cells (AECs), Ureaplasma parvum, and Sneathia spp. were also utilized.

Methods: The expression of TSLP, TSLPR, and IL-7Rα was evaluated in amniotic fluid or CAM by …

Sea-Phages Course-Based Undergraduate Research Experience For Creating A Biotechnology Workforce Development Pipeline, Daphne Fauber, Kari Clase, Carol Weaver

Graduate Industrial Research Symposium

Purdue University has been a member of the SEA-PHAGES (Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science) program since 2011. This program, created in conjunction with the Howard Hughes Medical Institute (HHMI), facilitates undergraduate curriculum for the authentic discovery of novel bacteriophages within the classroom. Since joining the program, undergraduates at Purdue have harnessed wet lab and bioinformatics principles to contribute over 200 previously uncharacterized bacteriophages and 25 novel genomes to the wider scientific literature. The SEA-PHAGES classes at Purdue have resulted in tangible professional deliverables for students through conference presentations and publications. Student outcomes also include transferable skills …

Anterior And Posterior Tongue Regions And Taste Papillae: Distinct Roles And Regulatory Mechanisms With An Emphasis On Hedgehog Signaling And Antagonism., Archana Kumari, Charlotte M. Mistretta

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Sensory receptors across the entire tongue are engaged during eating. However, the tongue has distinctive regions with taste (fungiform and circumvallate) and non-taste (filiform) organs that are composed of specialized epithelia, connective tissues, and innervation. The tissue regions and papillae are adapted in form and function for taste and somatosensation associated with eating. It follows that homeostasis and regeneration of distinctive papillae and taste buds with particular functional roles require tailored molecular pathways. Nonetheless, in the chemosensory field, generalizations are often made between mechanisms that regulate anterior tongue fungiform and posterior circumvallate taste papillae, without a clear distinction that highlights …

Mglu5 Inhibition In The Basolateral Amygdala Prevents Estrous Cycle-Dependent Changes In Cue-Induced Cocaine Seeking, Claire M. Corbett, Emily N. D. Miller, Jessica A. Loweth

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Drug associated cues are a common relapse trigger for individuals recovering from cocaine use disorder. Sex and ovarian hormones influence patterns of cocaine use and relapse vulnerability, with studies indicating that females show increased cue-induced craving and relapse vulnerability compared to males. In a rodent model of cocaine craving and relapse vulnerability, cue-induced cocaine seeking behavior following weeks of withdrawal from extended-access cocaine self-administration is higher in females in the estrus stage of the reproductive (estrous) cycle (Estrus Females) compared to both Males and females in all other stages (Non-Estrus Females). However, the neuronal substrates and cellular mechanisms underlying these …

Dpc29 Promotes Post-Initiation Mitochondrial Translation In Saccharomyces Cerevisiae, Kyle A. Hubble, Michael F. Henry

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Mitochondrial ribosomes synthesize essential components of the oxidative phosphorylation (OXPHOS) system in a tightly regulated process. In the yeast Saccharomyces cerevisiae, mitochondrial mRNAs require specific translational activators, which orchestrate protein synthesis by recognition of their target gene's 5'-untranslated region (UTR). Most of these yeast genes lack orthologues in mammals, and only one such gene-specific translational activator has been proposed in humans-TACO1. The mechanism by which TACO1 acts is unclear because mammalian mitochondrial mRNAs do not have significant 5'-UTRs, and therefore must promote translation by alternative mechanisms. In this study, we examined the role of the TACO1 orthologue in yeast. We …

Development Of A Method For Identifying And Quantifying Epicatechin In Cinnamon Extract Supplement Capsules, Danielle Valls

Annual Research Symposium

No abstract provided.

Effects Of Post-Translational Histone Modifications On Transcription Rate, Aaron Bohn

Annual Research Symposium

No abstract provided.

Biorisk Management, Laboratory Acquired Infections And Clinical Containment, Kashif Ali, Furqan Kabir, Esmeralda Meyer

Department of Paediatrics and Child Health

No abstract provided.

Nutrition Derived Advanced Glycation End Products Are Bio-Social Determinants Of Health That Inform On Cancer Disparities, David P. Turner, Victoria J. Findlay, Bradley A. Krisanits

Surgery Presentations

Objectives

Through their ability to perpetuate a reactive stroma, the objective was to define the increased nutritional bioavailability of advanced glycation end products (AGEs) as a pro-tumorigenic consequence of interrelated health inequity risk factors that can influence ancestry specific tumor biology.

Methods

In vivo, ex vivo, and in vitro models were used to define the molecular effects of nutrition associated AGEs on the ancestry specific tumor microenvironment and tumor growth.

Results

The PI’s work provides the first in vivo evidence supporting an oncogenic role for AGEs. In mouse allograft models, both the chronic consumption of AGEs and pre-treatment …

It Turned Into A Bioblitz: Urban Data Collection For Building Scientific Literacy And Environmental Connection, Kelly O'Donnell, Lisa Brundage

Publications and Research

In 2013, Macaulay Honors College redesigned its required science curriculum to focus on scientific literacy skills rather than content. Central to this shift was inclusion of a data collection event, a BioBlitz, to provide students with the basis for their own semester-long research projects. Students are teamed with naturalists in an urban green space to find as many species as they can in 24 h and to contribute to a global biodiversity database via the app iNaturalist. We have learned two important lessons: (1) developing an interdisciplinary curriculum with a high degree of experiential learning is more successful when both …

Effect Of Initial Infestation Of Zebra Mussels On Freshwater Drum Diets In Red Lake, Mn, Marissa M. Pribyl

Journal of Earth and Life Science

Zebra mussel Dreissena polymorpha veligers were first found in the Red Lake system in Beltrami County in 2017, however no adult zebra mussels have been documented. When zebra mussels first invade a system, fish often switch their prey use to this new abundant resource. Freshwater drum Aplodinotus grunniens is one of these fish species known to consume physidae and bivalves, including zebra mussels, using pharyngeal teeth, the molar-like grinders in the back of their throats. This research aims to identify any presence of adult zebra mussels in the system and determine freshwater drum diet composition and feeding patterns by analyzing …

Have Rusty Crayfish Affected The Diets Of Yellow Perch?, Matthew D. Kvam

Journal of Earth and Life Science

Freshwater ecosystems are some of the most susceptible to invasive species. It is one of the reasons they face the highest levels of species endangerment. Rusty crayfish Faxonius rusticus are one such invasive species, they fragment food webs and negatively affect native species. However, it seems as though native species are beginning to rely on them as a source of food. Yellow perch Perca flavescens, an important forage and game species, is one such species that is highly affected by these invasions. At smaller sizes yellow perch primarily feed on invertebrates whose populations are degraded by rusty crayfish. Therefore, the …

Major Biochemical Constituents Of Withania Somnifera (Ashwagandha) Extract: A Review Of Chemical Analysis, Ekram Hany Mohamed, Mahmoud Tareq Abdelwahed,, Maha A. Hegazy

Pharmacy

Ashwagandha (Withania somnifera) is a very popular medicinal herb originated mainly in India and found in the Middle East and parts of Africa. Ashwagandha has gained recognition as the most revered plant in Ayurvedic medicine. Studies indicated that it is used as anxiolytic, anti-inflammatory, antioxidant, adaptogen, memory enhancer, and possess an effect on neurodegenerative diseases. Additionally, it was revealed that the plant exerts antibacterial, antiviral, antitumor, aphrodisiac, and cardiovascular protection activities. Due to the biological and nutritional importance of nutraceuticals, particularly ashwagandha, and as part of the ongoing research of the natural products and its bioactive phytochemicals, this review aims …

Identification Of Novel Biosynthetic Gene Clusters Encoding For Polyketide/Nrps-Producing Chemotherapeutic Compounds From Marine-Derived Streptomyces Hygroscopicus From A Marine Sanctuary, Hannah Ruth Flaherty

Honors Theses and Capstones

Nearly one out of six deaths in 2020, around ten million people, were caused by cancer, making it a leading cause of death worldwide (WHO, 2022). This major public health issue, in addition to the rise of multidrug-resistant (MDR) pathogens, provides a high demand for the discovery of new pharmaceutical drugs to be used clinically to treat these conditions. The Streptomyces genus accounts to produce 39% of all microbial metabolites currently approved for human health, indicating its potential as an important species to study for antimicrobial and anticancer agents. The long linear genome of Streptomyces contains specialized sequences known as …

Examining The Oxidative Stress Effects In Rats Exposed To 2-Aminoanthracene In Utero And High Fat Diet Three Months After, Brianna A. Lanoix

Honors College Theses

Polycyclic aromatic hydrocarbons are organic compounds that are created synthetically or found in partially burned oil, gasoline, and coal. 2-aminoanthracene (2AA) is a common example of a PAH and can affect lipid and carbohydrate metabolism and resulting in inflammatory problems and oxidative stress. The objective of the research project is to investigate oxidative stress in relation to diabetes in rats exposed to 2AA in utero and a high fat diet later in life. Pregnant dams were separated into treatment groups based on their dosages of 2AA; 0 mg/kg (control), 50 mg/kg (low), 100 mg/kg (high). During the gestation -postpartum period, …

Investigation To Examine The Profile Of Auditory Brainstem And Hearing Thresholds Using Tone Burst Audiometry Brainstem Response In A Preclinical Migraine Model, Megan Huelsing

Graduate Theses/Dissertations

Migraine is a disabling neurological disease that is characterized by prominent auditory symptoms, including hyperacusis, which is defined as extreme sensitivity to sounds. The goal of this study was to investigate changes in hearing sensitivity and neural changes in the brainstem by measuring neural activity of the auditory brainstem in an established preclinical model of chronic migraine. To induce a chronic migraine state within the trigeminal system, male and female Sprague Dawley rats were subjected to three known human risk factors including neck muscle tension and REM sleep deprivation that promote latent sensitization, and exposure to a pungent odor, which …

A Diet Comparison Of Bluegill And Bluegill X Pumpkinseed Hybrids, Russell D. Zwiers

Journal of Earth and Life Science

Bluegill Lepomis macrochirus and pumpkinseed Lepomis gibbosus commonly prey upon macroinvertebrates but have been found to show different preferences to what type of macroinvertebrates. Hybrids of the two species have been found to display physical characteristics of both parents but prey selection of the hybrids compared to the parents is unknown. Therefore, the objective of this study was to analyze the diets of bluegill and bluegill x pumpkinseed hybrids to understand what each species’ preference is when it comes to food as well as finding how a hybrid’s diet compares to the pure breed of one of the two crossed …

Effects Of Velocity And Substrate Size On The Formation Of Zebra Mussel Druses, Ian Mott

Journal of Earth and Life Science

Zebra Mussels Dreissena polymorpha are an invasive bivalve species that are highly successful at dominating new environments. These invaders have been known to colonize substrate so thoroughly that all they have to attach to are members of their own species. When large mats of interconnected mussels completely cover a riverbed, it can have a drastic effect on the surrounding environment. This study serves to create a better understanding of the hydrologic parameters needed for Zebra Mussels to create these ecosystem-transforming colonies known as druses. Data was collected on a small stretch of the Upper Mississippi River in Northern Minnesota, previously …

Estimate Of Long-Term Phytoplankton Biomass In Lake Bemidji, William J. Peterson

Journal of Earth and Life Science

Phytoplankton are photosynthetic organisms that are the base of aquatic food webs. In a dimictic temperate lake such as Lake Bemidji, the environment adjusts with the changing water temperatures and sunlight levels. The phytoplankton in a dimictic lake are adapted to subsist in long term seasonal changes but the exact change to the number of phytoplankton in unknown. The focus of this study was to estimate the biomass of phytoplankton in a long term period including winter stratification and summer peak. From a period between March 2023 and October 2023 water samples were collected from the lake in the south …

Characterizing Cellular Injury And Inflammation With Aldob-/-Mouse Model Of Mafld, Andy Rashid

All Master's Theses

Metabolic-associated fatty liver disease (MAFLD) affects 1 in 3 people in the US and is a healthcare burden on the order of billions of dollars. MAFLD is characterized by pathological lipid accumulation in the liver, excess production of triglycerides and cholesterol, and export of LDL particles that raise serum lipid levels. These metabolic perturbations can lead to the development of liver fibrosis, chronic inflammation, and insulin resistance. Diagnosing MAFLD prior to fibrosis is crucial for successful intervention and prevention of advanced liver disease and other metabolic complications. Fructose-1Phospahte (F1P) accumulation from fructose overconsumption can drive hepatic lipogenesis and MAFLD progression. …

Cell Signaling And Stress Response In The Yeast Saccharomyces Cerevisiae: A Study Of Snf1, Scott E. Arbet Ii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Saccharomyces cerevisiae are yeast that are unicellular eukaryotic organisms that are well studied as a model organism for understanding fundamental cellular processes. The ability of yeast to sense nutrient availability is crucial for their survival, growth, and reproduction. Yeast cells use various mechanisms to sense and respond to nutrient availability, including transporter-mediated uptake, receptor-mediated signaling, and sensing of metabolites. The subcellular localization of nutrient-sensing components is crucial for yeast function in nutrient sensing and signaling. Protein complexes, such as the AMP-activated protein kinase (AMPK) pathway, in nutrient sensing and response, as well as the downstream effects of these pathways …

Amphisbaenian Head Movement And Burrowing Forces In Damp Granular Media, Jacob Newell

Williams Honors College, Honors Research Projects

Damp granular media is a difficult environment to study because it is both practically complex and it lacks equations which fully describe its behavior. In this study, an oscillatory lateral head movement and its effects while penetrating damp granular media were tested using a robophysical model. This experimental research was inspired by the burrowing behavior of the clade Amphisbaenia, a group of usually limbless squamates that employ a variety of different burrowing behaviors, but it can apply to a wide range of burrowers. This research could help with both human burrowing technologies and the further investigation of animal behaviors.

The Effect Of Alpha Lipoic Acid On Cardiac Autonomic Neuropathy In Danio Rerio, Sophia Scott

Williams Honors College, Honors Research Projects

Even though poor glycemic control puts diabetics at risk for a variety of disease complications, there are many barriers to achieving adequate blood glucose regulation. Diabetic cardiac autonomic neuropathy (CAN) is a progressive autonomic denervation of the cardiovascular system resulting from chronic hyperglycemia and characterized by orthostatic hypertension, sinus tachycardia, low heart rate variability, and fatal arrhythmias. Available CAN therapeutics are limited to symptom alleviation, and do not prevent or reverse cardiac nerve damage. Limited evidence from clinical trials suggests the antioxidant Alpha Lipoid Acid (ALA) prevents CAN progression (Lee et al., 2017; Serhiyenko et al., 2020; Tankova, Koev & …