Laboratory and Basic Science Research Commons^™

Viability Of Dentin In Rats Incisors : Fluid Movement In Pulpless, Endodontically Treated, And Normal Teeth In Rats, Maria Luisa Canales

Loma Linda University Electronic Theses, Dissertations & Projects

It is a well established fact that there is a fluid transport system through the dentin, however to the present time very little has been done regarding this system in teeth that have been endodontically treated.

The present work was conducted in vivo using the lower incisors of rats as the medium for the study.

A selected number of Sprague Dawley rats (36 animals), whose average age was 60 days, and whose body weight averaged about 180 grams, were given an anesthetic of Sodium Pentabarbitol, .05 to .07 ml. per 100 grams body weight. This anesthetic was injected intraperitoneally.

The …

Effect Of Post-Operative Use Of Chlorhexidine On Regeneration Of Bifurcation Defects In Dogs, Gary Bogle

Loma Linda University Electronic Theses, Dissertations & Projects

The daily post-operative use of topical chlorhexidine application has been compared to daily toothbrushing. Periodontal defects were experimentally created in the bifurcations of lower premolars in dogs. The defects were treated by surgical debridement. Chlorhexidine application and toothbrushing were performed post-operatively for six weeks on contralateral pairs of teeth. The amounts of connective tissue and bone regeneration were evaluated from microscopic sections of the bifurcation areas. No difference between chlorhexidine and tooth brushing was found for the connective tissue attachment. However, significantly more bone had regenerated in the chlorhexidine treated areas than the tooth brushed area.

New Laboratory For Virus Disease Studies, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Exotic diseases continually pose a threat to Australia's animal industries and the consequences of major outbreak could be disastrous.

If an outbreak should occur, it is essential that clinical diagnosis is confirmed without delay for control measures to be effective.

The completion of the new Virology Laboratory provides a building in which most exotic viral diseases could be handled with safety for diagnostic purposes in the event of an outbreak.

Effects Of Dietary Factors And Morphine Administration On Voluntary Alcohol Consumption And Dopamine Metabolism In Rats, Claudia Smithson

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of diet and dietary additives on voluntary alcohol consumption in male Sprague-Dawley rats was studied. Alcohol intake measured weekly and reported as ml of a 10% solution ingested per 100 grams body weight. A four week pre-experimental period was designed to determine individual tendencies to drink and thus to eliminate the high and low drinkers from the study. No difference was found in alcohol intake with either starch or sugar as the carbohydrate source; however, there was a greater weight gain ( p < .025) in those animals consuming sugar-based diets. Rats on poor-quality diets containing minimal amounts of vitamins and minerals continued to gradually increase alcohol consumption during the study. This diet, supplemented with coffee, caused the greatest significant increase in alcohol intake and the lowest weight gains. Addition of coffee to the diets significantly increased alcohol consumption (p< .005) but decreased growth rate (p < .0005) . However, when the poor quality diets were supplemented with vitamins and minerals, alcohol consumption was reduced by about 50% (p < .005) and weight gain was significantly increased (p < .0005).

The daily intraperitoneal administration of morphine sulfate significantly decreased alcohol intake (p < .02) and the per cent of total fluid consumed as alcohol (p < .02) but did not effect water consumption.

An attempt was …

The Comparative Histochemistry Of The Infective Oncospheres Of Oochoristica Osheroffi Meggitt, 1934, And Oochoristica Scelopori Voge And Fox, 1950, (Cyclophyllidea: Linstowiidae) With Notes On The Origins Of The Egg Membranes, Gary R. Coulter

Loma Linda University Electronic Theses, Dissertations & Projects

Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.

Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.

Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was …

Endodontic Sealing Properties Of Cavit, Chloro-Rosin Gutta Percha, N2, And Silver Cones Using Radioisotopes, Frank H. Beal

Loma Linda University Electronic Theses, Dissertations & Projects

The main purpose of this investigation was to test the endodontic sealing abilities of silver cones, N2, warm gutta percha, chemically softened gutta percha, laterally condensed gutta percha and Cavit under carefully controlled conditions. Evaluations were done by radiographic, macroscopic, microscopic, and autoradiographic examinations.

The four points which must be considered when evaluating the efficacy of sealing root canal systems, in vivo, were presented and discussed.

Cavit as a total endodontic filling material was presented, investigated and discussed.

An improvement of the chloro-rosin - gutta percha diffusion technique was presented.

The concept of surface as well as marginal leakage was …

Separation Of The Heavy Lanthanons From Egyptian Monazite Sand, John Nicholas Keramopoulos

Archived Theses and Dissertations

No abstract provided.

Vibrational Analysis Of Octahedral Molecules, Magdi Aziz Girgis

Archived Theses and Dissertations

No abstract provided.

The Constant Estrus Rat As A Source Of Viable Ova, John C. Drake

Morehead State Theses and Dissertations

A Thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by John C. Drake, Jr. on September 10, 1971.

Root Exposure And Its Effect On The Fluid Movement Within The Tooth, William J. Busch

Loma Linda University Electronic Theses, Dissertations & Projects

This study examined the fluid movement in dentin associated with the exposure of the root surface for varying periods of time ranging from 75 minutes to 30 days. Eight kittens received experimental defects on the roots of the canines. Just prior to sacrifice a fluorescing dye was injected intraperitoneally to make the fluid within the dentin visible under ultraviolet light. Frozen ground sections were prepared and examined. The results showed that the exposed side of the root had a reduced amount of fluid fluorescence when compared to the opposite side of the tooth which had an intact root surface. The …

The Degree And Time-Course Of Stress In The Patient Undergoing Cardiac Surgery, Hulda Esther Serra, Patricia Jo Yvonne Tygret

Loma Linda University Electronic Theses, Dissertations & Projects

An important nursing objective is to assist patients through stressful experiences. It has been assumed that cardiac surgery constitutes such a stress situation. For this reason, twenty-two patients undergoing cardiac surgery at Loma Linda University Hospital studied for the purpose of exploring the degree and time-course of were psychological stress.

The methodology consisted of administration of psychological tests before and after surgery; determination of urine catecholamines (UCA) before, during and after surgery; administration of a coping assessment tool before surgery, and a recall interview on the fifth day after surgery. The Multiple Affect Adjective Check List (MAACL)-Today form, and the …

The Duration Of The Effect Of Different Carbohydrates On Neutrophilic Phagocytosis, Judy L. Reeser

Loma Linda University Electronic Theses, Dissertations & Projects

In order to determine the duration of the effect of glucose, fructose, sucrose, honey, starch, and orange juice on neutrophilic phagocytosis, 10 subjects underwent a complete series of carbohydrate tolerance tests. Blood samples were drawn at specific time periods following ingestion of 100 grams of each carbohydrate. Blood was then analyzed for glucose and phagocytic index (the mean number of bacteria engulfed per leukocyte); a red blood cell and white blood cell count and differential white cell count were done also on about 1/2 of the samples. In addition, 7 other subjects participated in a fasting study in which no …

Cardioregulatory Role Of The Abdominal Ganglion Of Aplysia Californica, George Maeda

Loma Linda University Electronic Theses, Dissertations & Projects

An isolated heart-abdominal ganglion preparation of this mollusc was developed and used to study the cardioregulatory function of the ganglion and nerves. The heart was perfused with artificial sea water through an atrial cannula and the gastro-esophageal artery was catheterized for blood pressure monitoring. The presence and cardioregulatory effects of axons in the nerves was investigated by electrical stimulation. Fine-tipped glass microelectrodes inserted into ganglionic neurons were used to determine the relationship between the neuronal and cardiac activities.

The principal results are listed below.

1. Primarily inhibitory responses are obtained from both right and left connective stimulation, with some evidence for …

The Determination Of The Time Of Ovulation, First Cleavage, Subsequent Cleavage Divisions And Implantation In Female Mice Treated With Gonadotrophins, James William Weldon

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by James William Weldon in May of 1971.

Bacteriuria As A Precursor Of Intracatheter Encrustation, Namcy Moll

Loma Linda University Electronic Theses, Dissertations & Projects

The problem with which this study was concerned was the development of criteria that could be used to determine when urinary catheter changes were necessary. By determining when encrustation occurred, it was felt that some complications could be eliminated by changing catheters before obstruction occurred due to encrustation.

It was the purpose of this study to: 1) explore some indications for catheter change, 2) determine if encrustation of urinary catheters necessitates catheter change, and 3) estimate potential criteria for a routine time for catheter changes.

Forty-five catheters were examined after their removal from patients. Flow rates were determined on 33 …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

Histopathological Variations Of Periapical Granulomas And Radicular Cysts, Thanapen Kanchanasut

Loma Linda University Electronic Theses, Dissertations & Projects

The periapical dental granuloma and cyst is the most frequent sequela of pulpitis. These lesions are always intraosseous and often become quiescent after the acute phases are over, forming a space which primarily consists of chronic inflammatory tissue. This chronic inflammatory tissue usually appears as a one-centimeter area of punched out bone on the x-ray film and is referred to as a dental granuloma. Should epithelium choose to proliferate in this inflammatory area, it may undergo subsequent cystic degeneration forming the typical periapical cyst. While much has been written with regard to the etiology and treatment of the apical cyst …

Molecular Weight Determinations Of Some Salmonella Potsdam Bacteriophage Dna's, Winston H. Richards

Loma Linda University Electronic Theses, Dissertations & Projects

Two group A phages, P9c/2 and P4/2, were studied. Their density, sedimentation velocity, and biochemical properties were compared. Their densities were determined by density gradient centrifugation in 38.3% cesium chloride. Sedimentation velocity was used to determine their molecular weights. To accomplish this, the DNA of P9c/2 and P4/2 was labeled with tritium and T₂ DNA, labeled with ³²P, was used as a standard. The DNA of P9c/2 and P4/2 was separately mixed with T₂ DNA and centrifuged in a 0-15% sucrose gradient. The molecular weights were calculated using the formula D₂/D₁= (M_{2 …}

The Incidence Of Ichthyophthirius Multifiliis Fouquet 1876 In Fish From Two Freshwater Streams And Commercial Bait Shops Of Rowan County, Kentucky, Lois Irene Parsons

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Lois Irene Parsons on May 14, 1970.

A Study To Demonstrate The Immediate Destination Of An Embolus Formed In The Lumen Of A Peripheral Venous Catheter, Rosemarie Morikone Emori

Loma Linda University Electronic Theses, Dissertations & Projects

This study was designed to show the immediate destination of an embolus formed in the lumen of an intravenous catheter placed in a peripheral vessel. The hypothesis stated that clots injected into a peripheral vessel will lodge in the lungs. A subhypothesis was that the clot may lodge anywhere along the circulatory pathway.

Indications of need for the study as shown by the review of literature were the following: (1) the complications resulting from the frequent use of intravenous polyethylene catheters, (2) experimental studies that showed that clots were formed around intravenous catheters, (3) the evidence that intravenous catheters predispose …

Effects Of Diet And Stress On Gastric Lesions In Albino Rats And Guinea Pigs, Naomi Mckenzie

Loma Linda University Electronic Theses, Dissertations & Projects

Studies were conducted to (1) investigate whether any protective factor is present in fresh water-cured olives or fresh cabbage as compared with the heated supplements against stress-induced gastric lesions in rats maintained on a nutritionally adequate diet for six weeks, (2) determine the relative protective factor in water-cured olives as compared with cabbage, (3) study the effect of black pepper in combination with fresh cabbage and olives and heated cabbage and olives in rats that were fed the same nutritionally adequate diet, and (4) investigate the effects of the same dietary components—cabbage and olives--on histamine- induced gastric lesions in guinea …

Mandibular Nerve Regeneration Through Solid And Canalized Bone And Plaster Grafts, Lawrence D. Day

Loma Linda University Electronic Theses, Dissertations & Projects

Utilizing an extra-oral approach and sterile technique, left mandibular ostectomy was performed on five miniature pigs. The segment removed was 3 cm long and included the inferior border, mandibular canal and, in some cases, apices of a molar tooth.

Bone and plaster grafts (one solid and one canalized of each) were used to fill these defects. The fifth was left with no graft.

The objective was to learn the effect of each of these procedures on regeneration of the mandibular nerve across the defect. It was hypothesized that canals through the grafts would facilitate regeneration.

X-rays were taken periodically. The …

A Preliminary Investigation Of The Incidence Of Acanthocephalans In Freshwater Fish From Two Similar Streams In Rowan County, Kentucky, Langston D. Smith

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Langston D. Smith on July 23, 1969.

Some Aspects Of Cranial Bone Growth In The Puberal Macaca Mulatta Monkey: As Evidenced By Metallic Implants And Cephalometric Roentgenography And The Application Of A Geological Lapidary Technique For The Semi-Serial Thin Sectioning Of Undecalcified Bone, Lee Eugene Olsen

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to investigate cranial and facial bone growth in the puberal Macaca mulatta monkey over a six month period utilizing the methods of metallic implantation combined with serial cephalometric roentgenography and a bichromatic vital staining method.

The employment of the vital stains, alizarin Red S and anthracene blue, over the six month period of the study, failed to stain the actively growing bone as evidenced by gross and microscopic analysis of the undecalcified bone preparations.

Interimplant measurements were made across certain bone sutures and the following results were obtained: The zygomaticotemporal sutures showed marked growth …

A Cephalometric, Anatomic And Histologic Investigation Of The Effects Resulting From A Heavy Interrupted Orthopedic Force To The Maxilla Of The Macaca Mulatta Monkey, Roger Wayne Triftshauser

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine changes that occur in the craniofacial complex, when a heavy interrupted orthopedic force is employed to retract the maxilla in the post puberal rhesus monkey. Cephalometric, histologic and anatomic examinations were utilized to substantiate the findings.

Four female rhesus monkeys were used for experimentation. Two were experimental animals and the other two controls. Cervical retraction with headgear was applied over a period of 313 days and 159 days to the two experimental animals. Forces not exceeding 2.5 lbs. were found most effective.

The findings were as follows:-

1. A total posterior displacement of …

Hay And Pasture Digestibility Measured In The Laboratory, D N. Tulloch

Journal of the Department of Agriculture, Western Australia, Series 4

A recently developed " i n vitro" (laboratory) method of determining the digestibility of hay and pastures is giving good results in the Department of Agriculture's Dairy Laboratory.

Ecological Factors Controlling Population Distribution Of The Burying Beetle, Nicrophorus Orbicollis Say, Gerald L. Demoss

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Gerald L. DeMoss on July 11, 1968.

Concurrent Infections Of Schistosoma Mansoni And Trypanosoma Equiperdum In Mice, Venus Ee-Siriporn

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this investigation was to determine factors which may influence the survival time of mice infected with Trypanosoma equiperdum and the effects of T. equiperdum on mice infected with Schistosoma mansoni.

In order to maintain experimentally obtained standard hours of survival (88 hrs) in mice inoculated intraperitoneally with 150,000 T. equiperdum, it was found that trypanosomes removed from the donor rat should be kept at 25 C and all counts, dilutions of the organisms, and injection should be completed within 1 hour after removal from the donor host. Survival time of mice is increased as the …

A Study Of Alveolar Bone Loss And Two-Hour Postprandial Blood Sugar Levels, R. Leslie Arnett

Loma Linda University Electronic Theses, Dissertations & Projects

Two-hour postprandial blood sugar determinations were carried out on 500 patients referred for periodontal care. The mean percentage of alveolar bone loss around the mandibular bicuspid teeth was established for each individual. The subjects were then grouped according to blood sugar levels and the mean alveolar bone loss was calculated. The group was separated into male and female sub-groups and data was computed for these segments as well as the total sample. The data was evaluated in a similar manner for those subjects between 40 and 49 years of age.

No Chi-square determinations were statistically significant but significance in differences …

A Study Of The Extent Of Spread Of Infestation, Effects, And Possible Control Of The Tent Caterpillar In Lewis County, Kentucky, Edna Mae Love

Morehead State Theses and Dissertations

A thesis presented to the faculty of the Graduate School at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Arts by Edna Mae Love on May 22, 1968.