Laboratory and Basic Science Research Commons^™

Calling On Science: Making “Alternatives” The New Gold Standard, Melvin E. Andersen

in Vitro Research Models Collection

All of life’s great journeys start with a goal in mind! The 2007 NAS report, Toxicity Testing in the 21st Century – A Vision and A Strategy, has proposed a clear goal. This report envisions a not-so-distant future where all routine toxicity testing for environmental agents will be conducted in human cells in vitro evaluating perturbations of cellular responses in a suite of toxicity pathway assays. Dose response modeling would utilize computational systems biology models of the circuitry underlying each toxicity pathway; in vitro to in vivo extrapolations would use pharmacokinetic models, ideally physiologically based pharmacokinetic models, to predict human …

New Target Genes For Tumor Suppressors P53 And P73 In Regenerating Liver, Svitlana M. Kurinna

Dissertations and Theses (Open Access)

The p53-family of proteins regulates expression of target genes during tissue development and differentiation. Within the p53-family, p53 and p73 have hepatic-specific functions in development and tumor suppression. Despite a growing list of p53/p73 target genes, very few of these have been studied in vivo, and the knowledge regarding functions of p53 and p73 in normal tissues remains limited. p53+/-p73+/- mice develop hepatocellular carcinoma (HCC), whereas overexpression of p53 in human HCC leads to tumor regression. However, the mechanism of p53/p73 function in liver remains poorly characterized. Here, the model of mouse liver regeneration is used to identify new target …

Assessment Of Different Seed Mixture Planting Using Ground Beetles (Coleoptera, Carabidae) As Environmental Indicators Along A Disturbance Corridor In Lawrence County, Kentucky, Michelle M. Staley

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Michelle M. Staley on April 30, 2010.

Zooplankton Population Dynamics In The Upper Mississippi River-Lake System, Krystal Michaels

Honors Capstones

Capstone submitted as a graduation requirement for the BSU Honors Program.

Bioassessment Of Fish And Aquatic Macroinvertebrate Communities Of Laurel Creek In Rowan County, Kentucky, Audrey M. Richter

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Audrey M. Richter on April 2, 2010.

Wku Scholar, Pamela Napier, Editor-In-Chief

WKU Scholar

No abstract provided.

Toxicity Testing In The 21st Century: A Vision And A Strategy, Daniel Krewski, Daniel Acosta Jr, Melvin Anderson, Henry Anderson, John C. Bailar Iii, Kim Boekelheide, Robert Brent, Gail Charnley, Vivian G. Cheung, Sidney Green Jr, Karl T. Kelsey, Nancy I. Kerkvliet, Abby A. Li, Lawrence Mccray, Otto Meyer, Reid D. Patterson, William Pennie, Robert A. Scala, Gina M. Solomon, Martin Stephens, James Yager, Lauren Zeise

Experimentation Collection

With the release of the landmark report Toxicity Testing in the 21st Century: A Vision and a Strategy, the U.S. National Academy of Sciences, in 2007, precipitated a major change in the way toxicity testing is conducted. It envisions increased efficiency in toxicity testing and decreased animal usage by transitioning from current expensive and lengthy in vivo testing with qualitative endpoints to in vitro toxicity pathway assays on human cells or cell lines using robotic high-throughput screening with mechanistic quantitative parameters. Risk assessment in the exposed human population would focus on avoiding significant perturbations in these toxicity pathways. Computational systems …

Environmental Monitoring: Acoustic Wireless Sensors For Pest Detection, Adam Osseiran

ECU Research Week

Presented at a Showcase of Research at the School of Engineering.

Southern Lagoon And Village Of Gales Point, Belize, Ca: Water Uses, Water Quality, And Potential Health Impacts, Rasmi Nair, Ritchie Taylor

Impact Belize

An International Health Service Learning Program, Impact Belize 2010, was conducted by Western Kentucky University. Faculty and students worked closely with Gales Point villagers to gain an insight into the use of Southern Lagoon. The objective of the study was to assess fecal coliform levels in Southern lagoon, its primary and secondary contact use by villagers and its potential impact on health. Study methodology involved collection of water samples, both near shore and offshore, in the lagoon and incubating them using ColiQuant EZ method approved by EPA. A questionnaire was administered to villagers attending the WKU clinic to collect data …

The Current Scientific And Legal Status Of Alternative Methods To The Ld50 Test For Botulinum Neurotoxin Potency Testing, Sarah Adler, Gerd Bicker, Hans Bigalke, Christopher Bishop, Jörg Blümel, Dirk Dressler, Joan Fitzgerald, Frank Gessler, Heide Heuschen, Birgit Kegel, Andreas Luch, Catherine Milne, Andrew Pickett, Heidemarie Ratsch, Irmela Ruhdel, Dorothea Sesardic, Martin Stephens, Gerhard Stiens, Peter D. Thornton, René Thürmer, Martin Vey, Horst Spielmann, Barbara Grune, Manfred Liebsch

Experimentation Collection

No abstract provided.

Building Better Scientists Through Cross-Disciplinary Collaboration In Synthetic Biology: A Report From The Genome Consortium For Active Teaching Workshop 2010, Michael J. Wolyniak, Consuelo J. Alvarez, Vidya Chandrasekaran, Theresa M. Grana, Andrea Holgado, Christopher J. Jones, Robert W. Morris, Anil L. Pereira, Joyce Stamm, Talitha M. Washington, Yixin Yang

Biological Sciences Research

A common problem faced by primarily undergraduate institutions is the lack of funding and material support needed to adequately expose students to modern biology, including synthetic biology. To help alleviate this problem, the Genome Consortium for Active Teaching (GCAT) was founded in 2000 by Malcolm Campbell at Davidson College to bring genomics into the undergraduate curriculum. GCAT’s first tangible activity was to serve as a central clearinghouse both for the purchase and reading of DNA microarrays and for information on how to execute genomics experiments at undergraduate institutions. In response to the evolution of molecular biology in the last decade, …

Play And Adversity: How The Playful Mammalian Brain Withstands Threats And Anxieties, Stephen M. Siviy

Psychology Faculty Publications

Most mammals play, but they do so in a dangerous world. The dynamic relationship between the stresses created by their world and the activity of play helps to explain the evolution of play in mammals, as the author demonstrates in evidence garnered from experiments that introduce elements of fear to rats at play. The author describes the resulting fearful behavior and quantifies the fluctuation in play that results, and then he investigates how these are modified by increased maternal care or the use of benzodiazepines. In conclusion, he discusses how such research can help shed light on the neurobiology underlying …

Genetic Connections Between Neurological Disorders And Cholesterol Metabolism, Ingemar Bjorkhem, Valerio Leoni, Steve Meaney

Articles

Cholesterol is an essential component of both the peripheral and central nervous systems of mammals. Over the last decade, evidence has accumulated that disturbances in cholesterol metabolism are associated with the development of various neurological conditions. In addition to genetically defined defects in cholesterol synthesis, which will be covered in another review in this Thematic Series, defects in cholesterol metabolism (cerebrotendinous xanthomatosis) and intracellular transport (Niemann Pick Syndrome) lead to neurological disease. A subform of hereditary spastic paresis (type SPG5) and Huntington's disease are neurological diseases with mutations in genes that are of importance for cholesterol metabolism. Neurodegeneration is generally …

Observational Learning In Wild And Captive Dolphins, Deirdre Yeater, Stan A. Kuczaj Ii

Psychology Faculty Publications

Many non-human species imitate the behavior of others, and dolphins seem particularly adept at this form of observational learning. Evidence for observational learning in wild dolphins is rare, given the difficulty of observing individual wild animals in sufficient detail to eliminate other possible explanations of purported imitation. Consequently, much of the evidence supporting observational learning in dolphins has involved animals in captive settings. This research suggests that dolphins have an affinity for mimicry, and that they are more successful at observational learning if they choose to imitate another rather than being asked to do so. These results, combined with those …

High-Density Screening Reveals A Different Spectrum Of Genomic Aberrations In Chronic Lymphocytic Leukemia Patients With ‘Stereotyped’ Ighv3-21 And Ighv4-34 B-Cell Receptors, Millaray Marincevic, Nicola Cahill, Rebeqa Gunnarsson, Anders Isaksson, Mahmoud Mansouri, Hanna Göransson, Markus Rasmussen, Mattias Jansson, Fergus Ryan, Karin Karlsson, Hans-Olov Adami, Fred Davi, Jesper Jurlander, Gunnar Juliusson, Kostas Stamatopoulos, Richard Rosenquist

Articles

Background The existence of multiple subsets of chronic lymphocytic leukemia expressing ‘stereotyped’ Bcell receptors implies the involvement of antigen(s) in leukemogenesis. Studies also indicate that ‘stereotypy’ may influence the clinical course of patients with chronic lymphocytic leukemia, for example, in subsets with stereotyped IGHV3-21 and IGHV4-34 B-cell receptors; however, little is known regarding the genomic profile of patients in these subsets. Design and Methods We applied 250K single nucleotide polymorphism-arrays to study copy-number aberrations and copy-number neutral loss-of-heterozygosity in patients with stereotyped IGHV3-21 (subset #2, n=29), stereotyped IGHV4-34 (subset #4, n=17; subset #16, n=8) and non-subset #2 IGHV3-21 (n=13) and …

Identification Of Eurycea Using Cytochrome B, Karis A. Myers

Senior Honors Theses

Genomic sequencing is a powerful tool that has many applications for research, one of which is in the field of taxonomy and the identification of species. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. The Polymerase Chain Reaction is used to amplify a target DNA sequence, and the protocol for this procedure must be optimized for the specific sequence of target DNA. Primers must also be designed and modified for …

Bringing Toxicology Into The 21st Century: A Global Call To Action, Troy Seidle, Martin Stephens

Experimentation Collection

Conventional toxicological testing methods are often decades old, costly and low-throughput, with questionable relevance to the human condition. Several of these factors have contributed to a backlog of chemicals that have been inadequately assessed for toxicity. Some authorities have responded to this challenge by implementing large-scale testing programmes. Others have concluded that a paradigm shift in toxicology is warranted. One such call came in 2007 from the United States National Research Council (NRC), which articulated a vision of ‘‘21st century toxicology” based predominantly on non-animal techniques. Potential advantages of such an approach include the capacity to examine a far greater …

Scientific Autonomy And The 3rs, Bernard E. Rollin

Experimentation Collection

No abstract provided.

Fisheries Research Report No.196 - Management Of Bioeroding Sponges In Wild Stocks Of Pinctada Maxima In Western Australia, Sabine Daume, Jane Fromont, Anthony M. Hart

Fisheries Research Reports

Final FRDC Report – Project 2005/074

The Western Australian fishery is the only remaining significant fishery for pearl oysters in the world and comprises most of the exported pearl product of Australia, currently worth around $125 million annually. The majority of pearl shells used to culture pearls come from the pearling beds in the inshore waters near Broome. The fishery for pearl oysters preferentially targets smaller ‘culture’ shell (4-5 years age class, 120mm - 160mm shell length) that are more suitable for growing pearls, leaving larger MOP oysters (6+ age class, 175mm+) on the fishing grounds.

Silver-lipped pearl oysters (Pinctada …

Curated Exhibit: Histological Colorations: Explorations Into The Human Form, Laura J. Hall

Posters and Presentations

This exhibit showcased mixed-media prints by artist Kathamann, inspired by her experience as a histology technician. The 26 prints, featured in the exhibit, offer colorful and whimsical interpretations of human histological slides, exploring the intricate beauty of the human body at a microscopic level. Curated by Laura J. Hall, MFA, the exhibit was displayed at the Domenici Center for Health Sciences Education. Kathamann’s work reflects both her scientific background and artistic vision, bringing a new perspective to the study of the human form. September 25, 2009 - August 31, 2010

The Effects Of Fluorescent Tracking Powder On Oxygen Consumption In Salamanders Using Either Cutaneous Or Bimodal Respiration, Sarah A. Orlofske, Kristine L. Grayson, William A. Hopkins

Biology Faculty Publications

Fluorescent powder is gaining attention as an effective method for tracking terrestrial amphibian movements, particularly for species that are too small for conventional tracking equipment. The technique requires coating portions of an animal with fluorescent powder, releasing the animal, and following the trail of powder as it is progressively lost during movement. Recent studies have shown that fluorescent powder has no negative effects on survival or growth. However, a substance that coats the skin, a major respiratory organ in most amphibians, may have sublethal effects on performance and consequently behavior. We tested the effect of fluorescent powder application on the …

Mnte-2-Pyp And Radiation In A Prostate Cancer Model: Implications For Radiotherapy, Adeola Y. Makinde

Loma Linda University Electronic Theses, Dissertations & Projects

A major limitation of successful radiation therapy in cancer treatment is the increase in normal tissue damage as higher doses are used to achieve greater tumor destruction. Radiation dose optimization in cancer therapy requires achieving maximum tumor destruction with minimal damage to normal tissue Antioxidants have been shown to protect normal tissues against radiation damage, as radiation-induced tissue damage results predominantly from reactive oxygen species that directly damage cellular components. However, for effective use as normal tissue radioprotectants in radiotherapy, these antioxidants must not protect the tumors. Mn (III) tetrakis (N-ethylpyridinium-2-yl) porphyrin (MnTE-2-PyP) is a metalloporphyrin antioxidant that has been …

Research Choice And Finance In University Bioscience, David E. Ervin, Steven T. Buccola, Hui Yang

Economics Faculty Publications and Presentations

Academic bioscience's rising importance for downstream technology and growing private sector relationships have evoked substantial policy attention. We contribute to the scrutiny by asking how university bioscientists design and finance their research, with particular attention to the mutuality of research portfolio choice and funding success. The analysis requires consideration of other major influences on academic science, including scientific norms, human capital, and institutional environment. Drawing on a national survey of university bioscientists, we find that public financial support encourages more basic investigation and private support encourages more applied investigation. Yet downstream research is only moderately more excludable than upstream. Once …

Role Of No In The Regulation Of Cortisol Synthesis In Long-Term Hypoxic Sheep Fetal Adrenal, Tshepo Rothi Monau

Loma Linda University Electronic Theses, Dissertations & Projects

Our laboratory has demonstrated that sheep fetuses adapt to long-term hypoxia (LTH) by maintaining normal level of basal plasma cortisol despite increased adrenocorticotropic hormone (ACTH). But the mechanisms involved are not fully understood. Studies have shown that nitric oxide (NO) inhibits synthesis of steroid hormones in a variety of tissues, and in different animal species. Studies also showed that hypoxia increases both expression and activity levels of the nitric oxide (NO) generating enzyme, nitric oxide synthase (NOS), in certain tissues. We have here investigated the expression level and localization of NOS isoforms in sheep fetal adrenals. We also investigated the …

Hepatitis B Virus Subgenotypes D1 And D3 Are Prevalent In Pakistan, Anwar Ali Siddiqui, Saeeda Baig, Runu Chakravarty Runu Chakravarty, Tariq Moatter

Department of Biological & Biomedical Sciences

Background:

As the hepatitis B genotyping is important for assessing its clinical implications and geographical distribution, the sub-genotypes have been found useful for determination of specific genomic markers related to hepatocarcinogenesis. In Pakistan, there is no reported data on molecular evolutionary analysis of HBV. A study was, therefore, much needed to evaluate the spectra of mutations present in the strains prevalent here.

Findings:

To confirm specificity of PCR typing, phylogenetic analysis of the pre-S1 region and the divergence was studied through 13 sequences of 362 bp (accession number EF432765 – EF432777). A total of 315 serum samples, selected from HBsAg …

Personal Reflections On Russell And Burch, Frame, And The Hsus, Martin Stephens

Animal Welfare Collection

The coincidence of anniversaries associated with the publication of William Russell and Rex Burch’s The Principles of Humane Experimental Technique, the founding of the Fund for the Replacement of Animals in Medical Experiments (FRAME), and the establishment of the collaboration between FRAME and the University of Nottingham, provides an opportunity to reflect on Russell and Burch’s legacy and how it was carried forward by FRAME. The Principles, published in 1959, was the pioneering work in what later became the alternatives or Three Rs field of replacement, reduction, and refinement of animal use. Such was the book’s initial and undeserved obscurity, …

Food, Fibre And Flowers, Fuelling The Economy With Innovation And Science, Department Of Agriculture And Food, Western Australia

Horticulture published reports

The Department of Agriculture and Food is a world leader in the rapidly growing field of agri-food and fibre research and development, providing significant benefits for Westen Australian producers, the food industry sector and consumers.

The development of value-added products through innovation and science, which deliver real opportunities for the State's agri-food export capacity, is also important.

Seven examples of the Department's science and innovation.

Horseshoe Crab Spawning Survey Protocol, Jennifer Mattei

Biology Faculty Publications

The protocol lists supplies and clothing needed for the survey, as well as which data is to be collected and when and how it should be entered for the census of horseshoe crabs on the Recapture Data Sheet for 2009. Adopted from Cape Cod and Delaware Bay survey protocols.

An Analysis Of Illegal Bushmeat Availability In Local Restaurants Located In Voi, Kenya, Bridget A. Sutton

Masters Theses & Specialist Projects

The illegal bushmeat harvest has been identified as a reason for declining wildlife populations throughout much of Africa. For many years the trade was thought to exist primarily in Western Africa. The illegal use of bushmeat in Eastern Africa, including Kenya, went undocumented and unstudied. In 2004, the Born Free Foundation published a study which claimed illegal sale of wild game was substantial in butcheries throughout Nairobi, Kenya. In an effort to determine other markets of the commercial trade, the goal of this study was to analyze local restaurants in Voi, Kenya for illegal bushmeat sale. The town of Voi …

Towards An Action Spectrum For Photoentrainment Of The Chlamydomonas Reinhardtii Circadian Clock, Christa Gaskill

Masters Theses & Specialist Projects

No abstract provided.