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Examining The Behavioral And Phyisiolgical Effects Of D-Methamphetamine Administered Via E-Cigarette Aerosolization, Srikethan Mahavadi

Theses and Dissertations

Abstract

EXAMINING THE BEHAVIORAL AND PHYISIOLGICAL EFFECTS OF D-METHAMPHETAMINE ADMINISTERED VIA E-CIGARETTE AEROSOLIZATION

By Srikethan Mahavadi, B.S.

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science at Virginia Commonwealth University

Virginia Commonwealth University, 2021

Principal Investigator: Dr. Keith Shelton, PhD., Department of Pharmacology & Toxicology

E-cigarettes have become increasing popular worldwide. They are primarily used as a means to deliver nicotine to users, but these devices may also be used to administer a wide range of other substances including psychomotor stimulants such as d-methamphetamine. Methamphetamine has been widely abused throughout the United States …

Alcohol And Other Addictive Behaviors Among Bariatric Surgery Patients, Rachel M. Arnold

Theses and Dissertations

Research has repeatedly highlighted a high rate of comorbidity of addictive behaviors in both clinical and non-clinical samples. While polydrug use has received significant attention in recent years, less is known about rates of comorbidity with behavioral addictions such as eating, work, exercise, or sex. Individuals with a history of bariatric surgery may provide a unique opportunity to examine the potential co-occurrence of addictive behaviors. High rates of food addiction symptoms and changes in alcohol use patterns post-surgically have highlighted a potential gap in our understanding of bariatric patients and their needs post-surgically. Using a composite measure of addictive behaviors, …

Characterizing The Patterns, Predictors, And Processes Involved In Recovery From Substance Use Disorders, Rebecca L. Smith

Theses and Dissertations

Alcohol and drug use disorders are associated with significant cost to individuals, families, and society.Approximately 40-75% of affected individuals remit from alcohol use disorders (AUD). Although the development of AUD is well studied, recovery from AUD and factors that contribute to recovery are not as well characterized. With the definition of recovery evolving towards a process rather than an outcome, there is a need to better understand psychosocial functioning and quality of life among individuals at different points in their recovery journeys. Concurrently, additional research is needed to understand the interplay between sources of recovery capital, individual differences in risk …

Incorporating Ontological Information In Biomedical Entity Linking Of Phrases In Clinical Text, Evan French

Theses and Dissertations

Biomedical Entity Linking (BEL) is the task of mapping spans of text within biomedical documents to normalized, unique identifiers within an ontology. Translational application of BEL on clinical notes has enormous potential for augmenting discretely captured data in electronic health records, but the existing paradigm for evaluating BEL systems developed in academia is not well aligned with real-world use cases. In this work, we demonstrate a proof of concept for incorporating ontological similarity into the training and evaluation of BEL systems to begin to rectify this misalignment. This thesis has two primary components: 1) a comprehensive literature review and 2) …

Modification Of Culture Medium And Identification Of Microbial Contaminants For Improved In Vitro Propagation Of Freshwater Mussels, Raquel M. Wetzell

Theses and Dissertations

In vitro propagation efforts play an essential role in conserving and restoring threatened freshwater mussel populations by circumventing the need for a fish host. Across a broad range of taxa, transformation is induced with an artificial M199 medium and rabbit serum. However, such formulation may not be sufficient in culturing critical species with more specific physiological requirements. In this study, multiple serum mixtures were tested to improve in vitro transformation of two freshwater mussel species: yellow lampmussel (Lampsilis cariosa) and tidewater mucket (Atlantaconcha ochracea). These species were selected because they parasitize similar fish host species but …

Biotic Characteristics Of Managed And Unmanaged Coastal Dunes In The Outer Banks, North Carolina, Andrew E. White

Theses and Dissertations

Under future climate change and sea level rise scenarios, Natural and Nature-Based Features (e.g., dunes) that protect coastal habitat and infrastructure will be exposed to increased wave energy and storm surge. Understanding how these forces will impact coastal dunes is necessary for their continued use as protective features. Coastal dunes develop through feedback between vegetation and sediment deposition, a process complicated by species-specific growth rates and responses to burial. Wave flume studies have tested the effects of dune vegetation on erosion and found multiple plant organs across several functional types to be important for resisting erosion. Although dune building and …

A Thematic Analysis Of African American Fathers' Emotion-Related Beliefs And Behaviors, Deon Brown

Theses and Dissertations

This study explored African American fathers’ beliefs about and experiences with their own and their children’s negative emotions (i.e., parental meta-emotion philosophy and parental emotion socialization). Participants included 58 African American fathers in the Midwestern region of the United States between the ages of 29 and 40 (Mage = 30.94). 57 were biological fathers and one was a stepfather to toddlers between 24 and 31 months of age. Participants were invited to complete lab tasks, including the meta-emotion interview (MEI). The MEI is semi-structured interview of parents’ and their children’s negative emotion (i.e., sadness and anger). I selected a subset …

Using Individual Determinants To Predict Behavioral Health Service Use In Integrated Pediatric Primary Care, Alfonso L. Floyd Jr.

Theses and Dissertations

Mental health problems (e.g., attention, externalizing, internalizing) are commonly observed in youth and are often associated with significant impairment and an increased need for mental health services. Conceptual frameworks, such as Andersen’s Behavioral Model of Health Service Utilization, have proposed that several individual determinants (e.g., predisposing, enabling, and need factors) may influence service use for mental health problems among youth. Despite the glaring need for mental health treatment among youth with mental health problems, studies indicate that many affected youths do not receive mental health care. Integrated pediatric primary care (IPPC) settings provide an ideal setting for youth and families …

Towards The Systematic Evaluation Of Variable Modes Of Mechanical Conditioning On The Compositional, Microstructural And Mechanical Properties Of Engineered Tissue Vascular Grafts., Sarah Saunders

Theses and Dissertations

Coronary artery bypass surgery (CABG) remains one of the most common cardiac surgical procedures performed worldwide, frequently involving multiple bypasses, and commonly employing the patient’s internal mammary artery, radial artery, or saphenous vein. CABG is often not possible because native vessels were already employed in previous interventions or are diseased themselves. Synthetic vascular grafts are currently integral tools of vascular surgery and have had relative success in large-caliber applications providing substantial benefit to aortic or iliac grafting; however, small diameter (< 6 mm) arterial grafts have not yet translated into clinical effectiveness due to thrombosis and anastomotic intimal hyperplasia. ETVGs present an exciting potential alternative in vascular grafting by offering a blood vessel substitute that could exhibit all the functional characteristics of native vasculature. In addition to relieving supply limitations associated with coronary artery bypass surgery ETVGs are especially ideal for pediatric patients with congenital heart disease who require grafts that grow as they do, eliminating the need for reoccurring invasive surgeries.

Though the role of biomechanics in regulating cellular behavior promoting non-thrombogenicity, vasoactivity, and ECM synthesis and maintenance is well …

Investigating The Role Of Nlrp3 Inflammasome In Chemotherapy-Induced Peripheral Neuropathy, Urszula O. Warncke

Theses and Dissertations

Paclitaxel and oxaliplatin are commonly used antineoplastic drugs that cause chemotherapy-induce peripheral neuropathy (CIPN) in up to 90% of patients. The sensory symptoms of CIPN include numbness, loss of proprioception, tingling, spontaneous burning, shooting or electric shock-like pain, mechanical and thermal allodynia, and cold hypersensitivity among others. Affected patients struggle with daily activities, have a low quality of life, and might stop cancer treatment. Unfortunately, there are no effective prophylactic or therapeutic treatments for these side effects. Many studies point to the involvement of inflammation as a major contributor of CIPN pathology. We investigated the effects of genetic (NLRP3 …

Crosstalk Between Hippo And Rb Tumour Suppressor Pathways In Ovarian Cancer, Fatmata Sesay

Theses and Dissertations

The cell cycle is a highly regulated process that ensures the timely and accurate division of cells. Events of the normal cell cycle fall under two categories - positive and negative regulatory mechanisms. The first category, positive regulatory machinery, includes active protein complexes of cyclins in association with their partnering cyclin-dependent kinases (cyclin/CDK), which mediate series of phosphorylation events that relay a cell cycle progression from one stage to the next. The second category, the negative regulatory mechanisms, include the checkpoint controls consisting of the retinoblastoma (RB) family of proteins, some of which can form a transcriptional repressor complex DREAM. …

Branched Chain Amino Acid Strain State Monitoring With Raman Spectroscopy And Plasmonic Bowtie Nanoantenna Devices For Early Disease Detection, Caroline A. Campbell

Theses and Dissertations

This work centers on the development and the down-selection of nano-manufactured devices to be used in conjunction with Raman spectroscopy for probing a branched chain amino acid. The nano-manufactured devices integrate plasmonic nanoantennas for the purpose of amplifying molecular fingerprints, which are otherwise difficult to detect, through Surface Enhanced Raman Spectroscopy (SERS). Plasmonic nanostructures can be utilized for a variety of biomedical and biochemical applications to detect the characteristic fingerprint provided by Raman Spectroscopy. The nano-manufactured devices create an electric field that amplifies minute perturbations and raises the signal above background noise. This may provide a deeper understanding of signal …

Do Coverage Expansion And Patient-Centeredness Care Delivery Improve Patient Health Outcomes And Care Quality?, Shiva Salehian

Theses and Dissertations

Although correlated, improvement of patient health outcomes and quality of care is a result of health care delivery, performance, and healthcare policies that intend to impact health outcomes (Porter, 2010; AHRQ, 2015). This dissertation focuses on a special model of care delivery (Patient-Centered Medical Homes (PCMH)) and two programs (Addiction and Recovery Treatment Services (ARTS) and Medicaid expansion) to examine the correlation and impact of these programs on cardiovascular-related preventive care, healthcare expenditures, and health behavior hospitalizations as patient outcomes.

Artificial Intelligence-Powered Chronic Wound Management System: Towards Human Digital Twins, Salih Sarp

Theses and Dissertations

Artificial Intelligence (AI) has witnessed increased application and widespread adoption over the past decade. AI applications to medical images have the potential to assist caregivers in deciding on a proper chronic wound treatment plan by helping them to understand wound and tissue classification and border segmentation, as well as visual image synthesis.

This dissertation explores chronic wound management using AI methods, such as Generative Adversarial Networks (GAN) and Explainable AI (XAI) techniques. The wound images are collected, grouped, and processed. One primary objective of this research is to develop a series of AI models, not only to present the potential …

Bioinformatic Pipeline For Determining Terminal Repeats In The Human Cytomegalovirus Genome Assembled With Pacbio Long Read Sequences, Ahmed Al Qaffas

Theses and Dissertations

Human Cytomegalovirus (HCMV) is a member of the betaherpesvirinae subfamily of the Herpesvirus family. HCMV infection is common among adults worldwide, with an estimated seroprevalence of 66 to 95%, depending on the geographic region (Zuhair et al., 2019). Although most of the virus genomic content has been studied extensively, the terminal repeating region sequences remain understudied. Two main challenges hindered the study of the region: a) limitations of sequencing technologies; and b) misassembly of the repeats due to its complex nature. Here I show a novel bioinformatics pipeline that takes advantage of PacBio's long reads to resolve the challenges mentioned …

Effect Of Viscoelasticity On Cellular Morphology And Activity, Thomas J. Petet Jr

Theses and Dissertations

It has been well established that there is a link between substrate stiffness and cellular activities such as proliferation, migration, and differentiation. Less characterized is the link between the time-dependent viscosity of a substrate with those cellular activities. To explore this, PDMS substrates were created with predictably tunable stiffness and viscosity parameters. A simulated model was also developed in parallel to explore the potential effects of viscosity in a computationally predictive way. It was found that the inclusion of viscosity caused a major paradigm shift to a non-zero substrate equilibrium that was sensitive to increases in the substrate stiffness. Finally, …

Structure-Based Drug Discovery And Development Of Protein Structure Prediction Tools Using An Empirical Force Field, Noah B. Herrington

Theses and Dissertations

Traditional drug discovery has rapidly accelerated thanks to development of computational molecular modeling. The crucial component that these computational studies hinge upon is having a well-defined, and energetically favorable structure. Structures of proteins and ligands that meet these criteria are important for accurately simulating models used to study drug binding. To demonstrate the role of accurate structure simulation in the study of these events, this thesis presents, first, a story examining the problem of accurate structure modeling of ionizable residues within protein structures, specifically aspartic acid, glutamic acid, and histidine. I present our method, which uses the HINT force field …

Investigating The Potential Causal Relationship Between Free Will Belief And Well-Being, Courtney J. Alderson

Theses and Dissertations

This multi-study dissertation had four primary aims. My first was to add to the evidence base indicating a positive association between free will belief (FWB) and subjective well-being (SWB). My second aim was to develop a measure to assess the FWB theme referred to as the principle of alternate possibilities (PAP). To achieve the first two aims, I conducted two cross-sectional studies to further establish the FWB-SWB association and start the development and assessment of a new PAP FWB measure. In the first study (N=995), I hypothesized that an EFA of the new PAP items would produce at least a …

Secondary English Teachers’ Experiences Of Agency: Connections To Shifting Educational Contexts During Covid-19, Kristina Lee

Theses and Dissertations

The COVID-19 pandemic caused schools around the world to enter uncharted territory. Due to the unprecedented nature of the educational crisis, it was important to examine how teacher agency may have been affected. Teacher agency can have important implications for school climate, policy, and the experience of stakeholders. The main focus of this study was to cultivate an understanding of secondary English teachers’ perceptions of agency as they navigated teaching throughout the COVID-19 pandemic. An ecological framework was used to examine teachers’ experiences of agency in the context of COVID-19. The study utilized a basic qualitative design with in-depth interviews …

Assessing Medical Discrimination, Mistrust, And Healthcare Engagement As Predictors Of Covid-19 Vaccination Among Transgender And Gender Diverse Individuals, Shelby A. Smout

Theses and Dissertations

As of March 11^th 2020, the World Health Organization (WHO) declared COVID-19 a global pandemic. Early studies conducted among LGBT+ communities indicated that individuals of color and transgender individuals were more likely to express hesitation towards a possible COVID-19 vaccine. Such hesitation can be explained by historical medical mistreatment of people of color and LGBT+ communities which contributes to higher medical mistrust within these populations. The present study assessed vaccination behaviors among a sample of transgender and gender diverse individuals (N=385) and results indicated an association amongst experiences of discrimination in healthcare, medical mistrust, COVID-19 mistrust, barriers to vaccination, …

“There Was Some Sort Of Missing Middle”: Lived Experiences Of Jewish Youth With Intellectual And Developmental Disabilities, Aliza Lambert

Theses and Dissertations

Though religion and spirituality contribute to overall well-being (Koenig, 2012; Koenig & Cohen, 2002), social support (Biggs & Carter, 2016; Koenig & Cohen, 2002; Taub & Werner, 2016), quality of life (QOL; Myers & Sweeney, 2000), coping strategies (Krok, 2008), and lower rates of depression and anxiety (Brown et al., 2013; Young et al., 2000), religion and spirituality are seldom included in curriculum in counselor education and supervision doctoral programs (Adams et al., 2015; Henriksen et al., 2015). In the present study, the researcher uses a multiple-case study design to describe the lived experiences of Jewish youth with intellectual and …

Predicting Medication Adherence Patterns During The Maintenance Phase Of Treatment For Pediatric Leukemia And Lymphoma: A Model Of Individual- And Family-Level Factors, Stephanie M. Romo

Theses and Dissertations

Adherence during the maintenance phase of pediatric cancer treatment is critical to prevent relapse and ensure long-term, event-free survival. Yet, little research has been done to examine individual- and family-level factors that may relate to adherence during the maintenance phase of treatment, particularly among Latinx patients. This is surprising given findings demonstrating that children who miss more than 5% of their prescribed oral chemotherapy medicine, most commonly 6-mercaptopurine (6MP), are 2.5-2.7 times more likely to relapse than children who take 95% or more of their prescribed 6MP. Pediatric cancer patients face unique adherence challenges given the importance of family involvement …

Self- And Socially-Regulated Learning In Middle School Science Classrooms: A Multiple Case Study, Lauren Cabrera

Theses and Dissertations

Students must employ self-regulated learning (SRL) and socially-regulated learning (soRL) in the science classroom, which includes a wide array of independent and collaborative learning activities. However, little is known about how student SRL and soRL co-occur in students’ learning and how the classroom teacher influences that regulation in situ (Cabrera et al., in preparation; Panadero et al., 2015). This explanatory, sequential case study analyzes classroom video data from six middle school science classrooms. The study uses an integrated coding scheme that captures SRL and soRL behaviors, soRL modes, and targets of regulation (Greene & Azevedo, 2009; Hadwin et al., 2018; …

Protein Structure And Interaction: The Role Of Aromatic Residues In Protein Structure And Interactions Between Pyridoxine 5'-Phosphate Oxidase/Dopa Decarboxylase, Mohammed H. Al Mughram

Theses and Dissertations

Naturally developed proteins are capable of carrying out a wide variety of molecular functions due to their highly precise three-dimensional structures, which are determined by their genetically encoded sequences of amino acids. A thorough knowledge of protein structures and interactions at the atomic level will enable researchers to get a deep foundational understanding of the molecular interactions and enzymatic processes required for cells, resulting in more effective therapeutic interventions. This dissertation intends to use structural knowledge from solved protein structures for two distinct objectives.

In the first project, we conducted a bioinformatics structural analysis of experimental protein structures using our …

Perceived Discrimination And Mental Health Outcomes In College Students: The Mediating Role Of Preventive Health Behaviors And Social Support, Sarah C M Morton

Theses and Dissertations

Perceived discrimination has been linked to adverse mental health outcomes, increased risk-taking behaviors, and poor engagement in health promoting behaviors. College students may be especially susceptible to negative mental health outcomes associated with discrimination due to the unique stressors faced by young adults (e.g., prolonged transition to adulthood, onset of mental health disorders, changes in social support). The current study examined the mediating and moderating roles of health behaviors and social support on the association between perceived discrimination and mental health outcomes (e.g., anxiety, depression, suicidality) in college students. A total of 709 college students (42.8% White; 72.2% female; 30.2% …

Arts Integration Experiences Of Elementary Educators, Amy E. Jefferson

Theses and Dissertations

The purpose of this study was to examine how elementary teachers applied their professional learning to integrate arts in the classroom. The research questions guiding the study included: (1) What content, skills, and understandings do teachers transfer from professional learning to practice? and (2) How do teachers navigate the implementation of arts integration? Knowles' (1978) Adult Learning Theory was used to examine how meeting the needs of teachers as adult learners during professional learning influenced the implementation experience.

A case study design employing qualitative data collection methods such as focus groups, individual interviews, and the sharing of artifacts from practice …

Development And Optimization Of A Low Volume Air-Jet Dry Powder Inhaler (Dpi) Platform For The Delivery Of High Dose Aerosolized Powder To Infants, Connor Howe

Theses and Dissertations

The goal of this research project was to develop an infant air-jet dry powder inhaler (DPI) platform for high-efficiency nose-to-lung (N2L) aerosol administration to infants through optimization of individual system components followed by expansion of the system to higher doses and different formulations using a new approach. Primary challenges associated with aerosol delivery to infants using the N2L route include the extremely narrow extrathoracic airways, short inspiratory times (~0.2 sec), low safe airway pressure (H₂O), and very low volumes of air (~10 mL) available to form the aerosol. The infant air-jet DPI system was comprised of three main …

Special Education Teacher Retention: The Importance Of Teacher Working Conditions, Erica Ross

Theses and Dissertations

Teacher retention, or why teachers stay, is an important area of study considering the impact teacher attrition and turnover can have on student outcomes (Hirsch & Emerick, 2006). Teacher attrition and turnover are even more concerning for those that are special education teachers (SETs), and it is noted as a priority to address in the state of Virginia (Solenson et al., 2018). Through an adapted organizational theory lens, this study addresses the following aims: 1) Determine if there are differences in perceptions of teacher working conditions (TWCs) between special education teachers (SETs) and teachers of other content areas and 2) …

Authenticity, Care, And Relationships: Ethical Decision-Making In Criminal Justice Education, Robert B. Lehmann

Theses and Dissertations

Many events in recent history have demonstrated the need for addressing the training of law enforcement and other public servants commonly referred to as criminal justice professionals in the United States. Reckoning with the imperfect history of the criminal justice system, specifically the history of problematic relations with marginalized people, and developing a system that is better equipped to meet the needs of an equitable and just society is at the forefront of many discussions of criminal justice reform. Improving ethical decision-making training in criminal justice education is one avenue to addressing these needs for current and future professionals. Conceptually, …

An Analysis Of The Special Focus Facility Program And Nursing Home Quality, Annie S. Rhodes

Theses and Dissertations

The Special Focus Facility Program is an intensive program meant to rapidly support 88 of the lowest quality nursing homes in the United States, as determined by the Five-Star Quality Ranking system. There are also 435 facility candidates which are similarly low performing but not enrolled in the program. Academic literature has largely ignored this program, and the existing grey literature is more than a decade old and does not include COVID-19 data. Applying a political economy of aging framework and using a case matching methodology, Special Focus Facilities (SFF), Special Focus Facility Candidate (SFFc) nursing homes, and 5-star nursing …