Digital Commons Network^™

The Effects Of Patient Care In Relation To Toxic Workplace Environments, Kayla Fitzgerald

Creating Healthy Work Environments (CHWE)

Background: Bullying in the workplace setting is a significant issue that can severely impact one’s career. Bullying can be demonstrated in several forms- such as verbal abuse, isolation, intimidation, and physical harm. Specific to nursing practice, workplace bullying is shown to have negative effects on mental health, patient care, and overall job satisfaction. While there are no definite means that organizations will take complete action towards this ongoing issue, several implementations can be instilled to reduce the incidence of workplace bullying.

Methods: In this project, studies of negative work environments, specifically workplace bullying, and patient care were critiqued and synthesized. …

Exploring The Impact Of Intersectional Identity On Maternal Morbidity/Birth Outcomes Among Afro-Latinas In Florida, Alexa Parra, Cynthia Lebron, Michaela Larson, Yue Pan, Jonell Potter, Hudson Santos

Creating Healthy Work Environments (CHWE)

Background: The “Hispanic/Latina Paradox" is a phenomenon where Latina women and their infants have perinatal and birth outcomes similar to those of White women. This is often seen as a protective mechanism that helps reduce maternal health inequities. However, this paradox overlooks the fact that there are different subgroups of Latina women, including Afro-Latinas, who are often neglected when addressing racial disparities related to perinatal birth outcomes in the US. The intersectionality of multiple minority identities may put Afro-Latinas at a disadvantage in terms of perinatal health outcomes.

Objective: Determine differences in maternal morbidity, including pregnancy-related hypertension and diabetes, and …

Screening For Postpartum Depression At A Suburban Chiropractic Clinic, Emma Stedronsky, Mary Degrote Goering

Creating Healthy Work Environments (CHWE)

Background: The pregnancy and postpartum period are crucial times to evaluate for mental health conditions. The American College of Obstetrics and Gynecology (ACOG) recommends that providers screen for perinatal and postpartum depression (PPD) at least once during pregnancy and once during the postpartum period using a validated screening tool. A chiropractic clinic in the upper Midwestern United States identified an anecdotal increase in mental health concerns in their pregnant and postpartum patient population since the COVID-19 pandemic. This project utilized interprofessional collaboration by implementing PPD screening at a chiropractic office according to the recommended guidelines using the Edinburgh Postnatal Depression …

Aiw26s: Linkedin Optimization Using Ai, Saisumedha Billapati

Paul English Applied Artificial Intelligence (AI) Institute Publications

This workshop was part of the PEAAII and SAC AI Workshop Series at the University of Massachusetts Boston. The session focused on using artificial intelligence to improve LinkedIn profiles and professional branding. Participants learned how to use AI tools to enhance summaries, keywords, and overall digital presence for career development.

Alaska Earthquake Center Quarterly Review July-September 2025, Heather Mcfarlin, Beth Grassi, Austin Holland, Nate Murphy, Elisabeth Nadin, Carolyn Parcheta, Michael West

Alaska Earthquake Center Reports

This series of technical quarterly reports from the Alaska Earthquake Center (AEC) includes detailed summaries and updates on Alaska seismicity, the AEC seismic network and stations, fieldwork, our online presence, public outreach, and lists publications and presentations by AEC staff. Multiple AEC staff members contributed to this report.

Lymphatic Pumping Failure In The Arm Precedes Dermal Backflow And Breast Cancer-Related Lymphedema, Melissa B Aldrich, Meghan E Mcwain, Simona F Shaitelman, Rian E Kuhns, John C Rasmussen, Wendy A Woodward, Sarah M Desnyder, Wenyaw Chan, Eva M Sevick-Muraca

The Brown Foundation: Institute of Molecular Medicine

Background

Breast cancer patients who undergo regional nodal irradiation therapy (RNI) following neoadjuvant chemotherapy and mastectomy or lumpectomy with axillary lymph node dissection (ALND) frequently encounter breast cancer-related lymphedema (BCRL). Early detection and treatment can significantly improve outcomes. Previously, near-infrared fluorescence lymphatic imaging (NIRF-LI) detection of dermal backflow, or retrograde lymphatic flow into initial lymphatics, was shown to precede clinical BCRL diagnosis by 8–23 months. Herein, we hypothesize that lymphatic pump failure is responsible for dermal backflow and may be a target to prevent onset of BCRL symptoms.

Methods

Arm lymphatics in 51 patients with locally advanced breast cancer were …

Front Matter, Editors Of Comparative Labor Law & Policy Journal

Comparative Labor Law & Policy Journal

Volume 45, Issue 4

Non-Waivability In Labour Law: A Foundational Principle In Transition, Guy Davidov, Cynthia Estlund

Comparative Labor Law & Policy Journal

EMPLOYMENT RIGHTS SECURED IN LEGISLATION cannot be waived by employee consent. This is almost a trite proposition, a truism that labour lawyers and students usually take for granted.

Non-Waivability In Labour Law: An Australian Perspective, Joellen Riley Munton

Comparative Labor Law & Policy Journal

Australian industrial relations laws traditionally provided detailed mandatory standards through a system of arbitrated occupation-based awards. In the years since a shift to enterprise bargaining, the system has tolerated a greater level of flexibility, even in the way that legislated minima are framed. The options for waiving otherwise mandatory standards are, however, limited by procedural protections. The Fair Work Commission maintains a supervisory role to ensure that workers are “better off overall” as a consequence of their bargains. This article explains the many ways in which Australian labour standards permit flexible application, within constraints designed to preclude exploitative practices.

The Non-Waivability Principle: Possible Derogations From Collective Bargaining And Re-Assessment Of Individual Bargaining In Assisted Procedures, Elena Gramano, Silvio Sonnati

Comparative Labor Law & Policy Journal

This article examines the evolving landscape of Italian labour law, with a focus on the principle of non-waivability and its gradual adaptation to demands for flexibility and competitiveness. Traditionally, the Italian labour law framework has been characterised by a strong non-waivability regime, which protects employees from negotiating away their rights due to inherent power imbalances vis-à-vis employers. However, recent regulatory developments have facilitated a shift towards both collective and individual agreements that allow deviations from statutory provisions, including to the detriment of employees. The article analyses the implications of this trend, exploring key regulatory models that enhance the role of …

Waiver Of Statutory Employment Rights In The United Kingdom, Hugh Collins

Comparative Labor Law & Policy Journal

The common law endorses the principle of freedom of contract, but, with few exceptions, Parliament has explicitly prevented straightforward waivers of statutory employment rights. Nevertheless, for the sake of promoting settlements of claims by employees, it is surprisingly easy for individual employees to enter into agreements to compromise claims for breach of those rights without appropriate safeguards such as independent advice and freedom from pressure. Moreover, employers’ lawyers have demonstrated considerable ingenuity in finding ways to avoid the application of employment rights either by manipulations of employment status or by seeking contractual agreements on the facts of the case that …

Relaxation Of Mandatory Rules Through Collective And Individual Agreements In Japanese Labour Law, Ryuichi Yamakawa

Comparative Labor Law & Policy Journal

This article analyses the legal situation in Japan regarding the relaxation of or deviation from mandatory regulations in labour law through collective and individual agreements. Although many statutory labour laws in Japan have a mandatory effect over individual employment contracts, several statutory provisions provide that such mandatory regulations can be relaxed through collective agreements under certain circumstances, as is the case in other industrialised countries. However, Japan’s situation is unique insofar as the relaxation is permissible through collective agreements concluded by non-union (individual) representatives of a majority of employees in the absence of union representatives who have been elected by …

Derogability By Collective Agreements In Germany And The Presumption, Eva Kocher

Comparative Labor Law & Policy Journal

The promotion of derogability clauses, which allow collective agreements to deviate from statutory protection to the detriment of employees, has been an issue in the political debate on German labour law for some 20 years. While such derogability clauses can effectively allow for flexibility at the company or sectoral level, there are differing views on their suitability for promoting collective bargaining. This article highlights the paradox of such derogability clauses: They are legitimised by the presumption of fairness that underpins collective autonomy; at the same time, they create incentives for employers that challenge the very presumption of fairness. All in …

The International Covenant On Economic, Social And Cultural Rights And The Employment Relation By Klaus Lörcher, Niklas Bruun, Ana Teresa Ribeiro, Hart Publishing, 2025, Manfred Weiss

Comparative Labor Law & Policy Journal

AFTER HAVING PUBLISHED THREE ENLIGHTENING BOOKS on the European Convention on Human Rights (ECHR), on the European Social Charter (ESC), and on the Charter of Fundamental Rights of the European Union (CFREU), the authors of the Transnational Trade Union Rights Expert Network of the European Trade Union Institute have now transcended the European territory and presented a publication on a global scale.

Derogability From Swedish Statutory Labour Law, Birgitta Nyström

Comparative Labor Law & Policy Journal

The possibility for the social partners to derogate from statutory legislation in the area of labour and employment law is an important characteristic of the so-called “Swedish model” for industrial relations and labour market regulation. With a system built on collective bargaining and collective agreements, and where legislation in the labour law area was rather scarce until the 1970s, freedom for the social partners is a core element. The possibility to derogate from certain parts of statutory labour law by collective agreements also gives room to adapt rules for different kinds of businesses and trades. Sweden is a member of …

University Placed-Based Community Engagement And Adult Learners: A Field Report In Mutual Flourishing, Elisabeth Stevens, Kate Witvliet, Abbie Lipsker, Craig Mattson

University Faculty Publications and Creative Works

Although higher education increasingly focuses on both community engagement and adult learners, community-engaged research rarely considers them together. This article bridges the gap in the literature between undergraduate adult learners and community engagement. Providing one example of a partnership that considers adult learners as necessary partners for community engagement, Calvin University’s 49507 Initiative is a place-based community educational project in southeast Grand Rapids, Michigan, focusing on the goals and needs of adult learners in the 49507 zip code and adjacent neighborhoods. Through 12 focus groups in the community, adult residents discussed their interests in higher education, identified barriers to higher …

Evaluating The Implementation Of Oregon's Dsm-5-Informed Autism Eligibility Revisions: Interest-Holder Perspectives And Policy Implications, Bradley Josef Hendershott

Dissertations and Theses

Under the Individuals with Disabilities Education Act (IDEA), states establish their own autism spectrum disorder (ASD) eligibility criteria, resulting in substantial interstate variation and frequent misalignment with medical diagnostic standards. In 2019, Oregon revised its ASD eligibility criteria by directly incorporating DSM-5 diagnostic criteria, reflecting proponents' theory of action that this alignment would improve conceptual clarity, identification accuracy, and coherence across educational and medical systems. While an early implementation study documented generally positive early effects reported by evaluators, no research has systematically examined whether the DSM-5 alignment achieved its anticipated effects, how the revised criteria function after multiple years of …

Micro-Refuges Or Ecological Traps: Context-Dependent Effects Of Rock Pools On Intertidal Biodiversity Across Latitudes, Louise B. Firth, Andrea Desiderato, Antony M. Knights, J. David Aguirre, Juan C. Astudillo, Juan C. Azofeifa-Solano, David T. Bilton, Su Yin Chee, Hartvig C. Christie, Ronaldo A. Christofoletti, Amelia Curd, Katherine A. Dafforn, Megan N. Dethier, Yun Wei Dong, Gabin Droual, Stanislas F. Dubois, Free Espinosa, Ally J. Evans, Andy Foggo, Stephen J. Hawkins, Mick E. Hanley, Ben P. Harvey, Eliza Heery, Seokwoo Hong, Amanda R. Hsiung, Tommy T.Y. Hui, Mar Humet, Katrin Iken, Soniya B. Jethva

School of Biological and Marine Sciences

AimWe investigated how local-scale environmental heterogeneity influences biodiversity patterns across broad biogeographic gradients, using intertidal microhabitats as a model system within one of the most environmentally stressful ecosystems on Earth.LocationIntertidal habitats at 26 locations (two rocky shore sites per location) across six continents, spanning 98° of latitude (38°S to 60°N).Time Period2019–2022.Major Taxa StudiedAlgae, sessile and mobile invertebrates.MethodsWe compared biodiversity and thermal environments across contrasting microhabitats (rock pools and adjacent emergent rock) along a latitudinal gradient, sampling during environmentally ‘milder’ and ‘harsher’ periods. Biodiversity was quantified using multiple richness metrics (mean, total, unique taxa) and functional diversity.ResultsMicrohabitat differences strongly influenced biodiversity …

S6k1 Modulates Stat3 Activation To Promote Resistance To Radiotherapy In Lung Cancer, Ali Calderon-Aparicio, Noelle Francois, Tyler Grenda, Shan Xu, Olugbenga Okusanya, Jun He, Nicole L Simone

Department of Radiation Oncology Faculty Papers

Radiotherapy is a mainstay in the management of locally advanced lung cancer; however, intrinsic and acquired radioresistance contribute to poor prognosis. S6K1, a serine/threonine kinase, regulates cell growth, protein synthesis, and survival, and is increased in tumors, which is linked to enhanced survival under therapeutic stress, including radiation. The mechanisms, however, are not fully understood. This study investigates the role of S6K1 in lung cancer radioresistance and the mechanisms involved. Intrinsic radioresistance in lung cancer cells was associated with increased S6K1 activation. Pharmacologic inhibition or genetic deletion of S6K1 enhanced radiosensitivity both in vitro and in vivo, highlighting the therapeutic …

Validating Uas-Based Ndvi Data With Satellite Landsat Imagery For Bald Eagle Habitat Prediction In The Del Rio Springs Ecosystem, Noah Morales, Ronny Schroeder, Elise Anderson

Student Works

Vegetation health is commonly assessed using the Normalized Difference Vegetation Index (NDVI), which can be derived from multispectral sensors operating at different spatial resolutions. Validating NDVI products across sensor platforms is essential to determine their reliability for environmental monitoring and habitat assessment. This research compares NDVI derived from moderate-resolution satellite imagery and high-resolution unmanned aircraft system (UAS) imagery collected over the same study area. Landsat imagery, provided through the joint USGS–NASA mission, was used to represent satellite-based vegetation patterns, while high-resolution multispectral data were acquired using a MicaSense sensor mounted on a UAS to capture fine-scale vegetation detail.

NDVI values …

Managing Acute Myocardial Infarction In Remote Oman: Real-World Data From Al Wusta Governorate, Thuraiya Malik Abdullah Al Harthi, Ibrahim Al Mahrouqi, Amin Elbanna, Karema Hamed, Mohammed Abushilla, Venkatesh Kumar, Maryam Al Nabhani, Hasina Al Harthi, Abdulmunim Al Farsi, Adil Al Mahrouqi

Sultan Qaboos University Medical Journal

Objectives: Acute ST-segment elevation myocardial infarction (STEMI) is a time-sensitive cardiac emergency requiring prompt reperfusion therapy. Management and outcomes in remote areas are underexplored. This study explored the STEMI reperfusion network in Al Wusta governorate in Oman and evaluated the effectiveness of pharmacological thrombolysis in general practitioner (GP)-led, resource-limited hospitals. Methods: This retrospective study reviewed administrative health registries and medical records of patients presenting with STEMI to hospitals in the Al Wusta governorate between June 2017 and December 2024. Response to thrombolysis was defined as 50% resolution of ST-segment on electrocardiogram. Outcomes post-thrombolysis were prespecified. Multivariate logistic regression was used …

Remnant Cholesterol And Atherosclerotic Cardiovascular Disease Risk In Populations With Different Low-Density Lipoprotein Cholesterol Elevations: A Prospective Cohort Study, Hong Zheng, Guanlin Chen, Zhenyu Huo, Yulong Lan, Yuxian Wang, Peng Fu, Weiqiang Wu, Haixiang Zheng, Kuangyi Wu, Zegui Huang, Dan Wu, Shouling Wu, Youren Chen

Research outputs 2022 to 2026

Background: Low-density lipoprotein cholesterol (LDL-C) and remnant cholesterol (RC) are risk factors for atherosclerotic cardiovascular disease (ASCVD). However, the extent to which differences in RC levels affect ASCVD risk in populations with varying degrees of LDL-C elevation remains unclear. This study aimed to investigate whether RC can provide additional risk stratification value across different sexes, ages, and elevated LDL-C statuses. Methods: This study included 12 743 elevated LDL-C participants (LDL-C ≥3.4 mmol/L) and 50 073 age- and sex-matched non-elevated LDL-C controls from the Kailuan Study. Elevated LDL-C participants were categorized by RC levels into <0.5, 0.5 to <1.0, and ≥1.0 mmol/L subgroups. Kaplan-Meier curves and Cox proportional hazards models were used to assess the relationship between RC levels and ASCVD risk across different sexes, ages, and high LDL-C statuses. Results: During a median follow-up of 12.8 years, 1686 elevated LDL-C participants (13.2%) and 5252 non-elevated LDL-C participants (10.5%) developed ASCVD. In the borderline-high LDL-C group (3.4 ≤ LDL-C < 4.1 mmol/L), those with the lowest RC levels showed no significant risk difference compared with controls (hazard ratio [HR], 1.03 [95% CI, 0.93-1.13]), and this pattern remained consistent across different sexes and ages. In contrast, in the high LDL-C group (LDL-C ≥4.1 mmol/L), even when RC was at the lowest level, ASCVD risk remained significantly higher than that of controls (HR, 1.20 [95% CI, 1.02-1.41]). CONCLUSIONS: In the borderline-high LDL-C population, those with the lowest RC levels showed no significant risk difference compared with controls, and this pattern remained consistent across different sexes and age subgroups. In the high LDL-C population, even when RC was at the lowest level, ASCVD risk remained significantly higher than that of controls.

Syndecan-1-Targeted Therapeutic Antibody Impairs Macropinocytosis And Elicits Antitumor Immunity In Pancreatic Cancer, Zecheng Yang, Madelaine S Theardy, Shuaitong Chen, Yongkun Wei, Mitsunobu Takeda, Yue Zeng, Xiaofei Wang, Jun Yao, Jennifer Li, Prapassorn Thirasastr, Jangho Park, Yangxi Zheng, Long T Vien, Khalida M Wani, Huamin Wang, Sisi Gao, Tim Heffernan, Lawrence Kwong, Ignacio I Wistuba, Laura Bover, Giulio F Draetta, Haoqiang Ying, Wantong Yao

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest malignancies, with a 5-year survival rate of just 13%. While the development and early clinical use of small molecules targeting oncogenic KRAS mutations, key drivers of PDAC, have shown promise, resistance to these targeted therapies remains a significant challenge. We recently identified Syndecan-1 (SDC1), a highly expressed heparan sulfate proteoglycan, as a critical KRAS effector protein that promotes nutrient salvage and tumor growth. Here, we report the development of a human-specific monoclonal antibody (anti-SDC1 mAb) that inhibits PDAC cell proliferation in vitro and suppresses PDAC tumor growth in vivo. Mechanistically, …

Tumor Microenvironment Transcriptional Activity Enables Robust Stratification Of Chemotherapy Response In Triple-Negative Breast Cancer, Yaoyi Dai, Xiaoxi Pan, Shuai Guo, Shuangxi Ji, Shaolong Cao, Matthew D Montierth, Yujie Jiang, Jeffrey T Chang, Leming Shi, Shabnam Shalapour, Gloria V Echeverria, Lucy Yates, Johan Staaf, Bora Lim, Yinyin Yuan, Wenyi Wang

Faculty, Staff and Student Publications

Triple-negative breast cancer (TNBC) exhibits heterogeneous treatment responses, yet molecular subtypes based on predefined biological pathways show limited prognostic value. We introduce tumor-specific total mRNA expression (TmS), a pathway-agnostic deconvolution metric derived from matched RNA/DNA sequencing, as a robust stratification tool. Analyzing 575 TNBC patients across Western and East Asian populations, TmS outperforms established subtypes in predicting chemotherapy outcomes, stratifying patients into high TmS with favorable prognosis and low TmS with poor prognosis. Stromal enrichment with immune exclusion emerges as a universal feature of chemotherapy-resistant low-TmS tumors across all cohorts. Population-specific features distinguish Asian cohorts: high-TmS tumors exhibit cell cycle-driven …

Expectant Management Vs Medication For Patent Ductus Arteriosus In Preterm Infants: The Pda Randomized Clinical Trial, Matthew M Laughon, Sonia M Thomas, Kristi L Watterberg, Kathleen A Kennedy, Martin Keszler, Namisavayam Ambalavanan, Alexis S Davis, Jonathan L Slaughter, Ronnie Guillet, Tarah T Colaizy, C Michael Cotten, Megan A Dhawan, Carl L Bose, Jennifer Talbert, Sarah Smucny, William E Benitz, Matthew A Rysavy, Robin K Ohls, Mariana C Baserga, Sara B Demauro, Mambarambath Jaleel, Wesley M Jackson, Waldemar A Carlo, Karen M Puopolo, Anna Maria Hibbs, Anup Katheria, Pablo J Sánchez, Carl T D'Angio, Ravi M Patel, Beth Ann Johnson, Valerie Y Chock, Abhay J Bhatt, Stephanie L Merhar, Ryan Moore, Abbot R Laptook, Sarvin Ghavam, Janell Fuller, Shilpa Vyas-Read, Stephen D Kicklighter, Baiba Steinbrekera, Kathryn Anderson, Praveen K Chandrasekharan, Myra H Wyckoff, Cassandra Montoya, Abhik Das, Barbara Do, Samantha Chang, Rosemary D Higgins, Michele C Walsh

Faculty, Staff and Student Publications

Importance: The management of patent ductus arteriosus (PDA) in preterm infants is controversial.

Objective: To determine whether expectant management compared with active treatment of a protocol-defined PDA in preterm infants decreases the incidence of death or bronchopulmonary dysplasia (BPD).

Design, setting, and participants: A randomized clinical trial including infants born at 22 to 28 weeks' gestation and diagnosed with a protocol-defined PDA between the age of 48 hours and 21 days at screening. The trial was conducted from December 2018 to December 2024 at 33 hospitals within the National Institute of Child Health and Human Development Neonatal Research Network. The …

Regalado V. Hughes Avenue Corp. And The Department Of Housing Preservation And Development Of The City Of New York

All Decisions

In this HP proceeding, the tenant moved for civil contempt and civil penalties against the landlord for failing to comply with a consent order to correct HPD violations, including those for mold and lead-based paint. The court granted the tenant's motion, finding the landlord in civil contempt for non-compliance with the order. The landlord's arguments, including a vague superintendent's affirmation and an unauthenticated D.H.P.D. repair bill, were insufficient to rebut the presumption that violations persisted. The court imposed a $350 per day fine, payable to the tenant, until outstanding "C" and "B" violations are corrected, and referred the calculation of …

17 Pennsylvania Avenue Hdfc V. Ventura

All Decisions

In this holdover proceeding concerning an HDFC, the Civil Court of the City of New York granted the tenant's motion for summary judgment, dismissing the case. The landlord sought to evict the long-term tenant, alleging good cause based on consistent failure to pay rent. However, after applying DRIE credits, the landlord's records only showed three months of unpaid rent totaling $1,500 from 2020. The court, citing precedent and emphasizing the affordable housing purpose of HDFCs, found that this minor, unpursued debt from several years prior did not constitute good cause for eviction. The tenant's counterclaim for harassment was severed.

Associations Between The Microbiome And Immune Responses To An Adenovirus-Based Hiv-1 Candidate Vaccine Are Distinct Between African And Us Cohorts, Yuhao Li, Daniel J Stieh, Lindsay Droit, Andrew Hyoungjin Kim, Rachel Rodgers, Kathie A Mihindukulasuriya, Leran Wang, Maria G Pau, Olive Yuan, Herbert W Virgin, Dan H Barouch, Megan T Baldridge, Scott A Handley

2020-Current year OA Pubs

Optimization of prophylactic vaccine regimens to elicit strong, long-lasting immunity is an urgent need highlighted by the COVID-19 pandemic. Stronger vaccine immunogenicity is frequently reported in individuals living in high-income countries compared to individuals living in low- and middle-income countries. While numerous host genetic and immune factors may influence vaccine responses, geographic restrictions to vaccine effectiveness may also be influenced by the intestinal microbiota, which modulates host immune systems. However, the potential role of the gut microbiota on responses to HIV-1 vaccines has not yet been explored. We analyzed the bacteriome by targeted 16S sequencing and the virome by virus-like …

Cannabis And Psychedelics Among U.S. Young Adults: Use, Messaging Exposure, Perceptions, And Legalization Support, Carla J Berg, Cassidy R Loparco, Darcey M Mccready, Laura C Schubel, Patricia A Cavazos-Rehg, Erin Kasson, Shriya Thakkar, Diane M Ndisebuye, Y Tony Yang

2020-Current year OA Pubs

This study assessed the correlates of cannabis and psychedelic use and legalization support among young adults in the United States (US). Using 2025 data among adults ages 18-34 (

Accurate Interpretation Of Within-Host Dissemination Using Barcoded Bacteria, Rachel T Giorgio, My T Le, Ting Zhang, Caitlyn L Holmes, Karthik Hullahalli

2020-Current year OA Pubs

Bacterial dissemination across tissues is a critically important process influencing infection outcomes. Monitoring within-host dissemination is challenging because conventional measures of bulk bacterial burden cannot distinguish between lineages that are shared between tissues and those that replicate locally. This limitation can be overcome using barcoded bacteria, where deep sequencing of the barcode locus and comparisons of barcodes between tissues define which lineages spread within the host. Numerous studies have used barcoded bacteria to generate high-resolution maps of dissemination. However, since multiple cells in the infectious inoculum can contain identical barcodes, inferences about dissemination can be confounded when distinct lineages from …