Digital Commons Network^™

Molecular Studies To Evaluate Variegation Of Philodendron Var. Birkin - Are Genetic Mutations Responsible For Variegation In Philodendron Birkin?, Muira C. Arndt, Isabelle R. Gile, Shekinah J. Beerman

Research & Creative Achievement Day

Plant tissue culture and molecular genetic techniques were used to analyze the instability of the genome in philodendron var. 'Birkin'. This plant has variegated, white, green, and greenish-red leaves (that have lost their variegation) on the same plant. Did genetic mutations occur in meristematic sectors of variegated leaves that have reverted to green coloration, or is it an epigenetic change?  PCR and sets of RAPD plastid primers were used to determine if the presence or absence of a PCR product correlated with differences in leaf color and variegation (white, green-white, variegated, reddish green or green leaves). A DNA isolation procedure …

Student Laboratory For Synthesis And Characterization Of Proton Exchange Membranes, Christiana Hoyt

Research & Creative Achievement Day

The primary goal of this research was to improve the current Proton Exchange Membrane (PEM) Synthesis and Characterization laboratory exercise for CHEM 411-Synthesis and Characterization of Materials. Intended improvements to this lab included measuring conductivity of the PEM by finding the best procedure for creating the conductivity cell through both research and experiments in order to add electrochemical impedance spectroscopy in the characterizations for the lab. Commercial membranes, such as Nafion, have multiple issues: high cost, high methanol permeability, poor function at elevated temperatures and at low humidity being some of them. Using PEEK to synthesize PEMs can be used …

Degradation Of Ovulation Inhibitor Estrogens Using Hplc Chromatography, Jenna Holst

Research & Creative Achievement Day

In recent years, there has been a rise in observed intersex aquatic species along with a rise of reproductive issues in humans. This has been linked to the endocrine disrupting properties of over 200 chemicals that have estrogenic activity and persist in aquatic environments. The focus of our research is the estrogens that are used in hormonal birth control as ovulation inhibitors. Between 2015-2017, 14% of US women aged 15-49 were using hormonal birth control. According to a study published in 2002, three estrogens that are used in hormonal birth controls as ovulation inhibitors, 17α-ethynyl estradiol, mestranol, and 19-norethisterone, occur …

Purification And Characterization Of Vrk2 For Further Research, Steven Richter

Research & Creative Achievement Day

Kinases represent a vast and varied group of proteins, having more than 500 members in human beings alone. They have been found to be involved in various signal transduction pathways, ranging from embryonic development to apoptosis. The vaccinia-related kinases (VRKs) are a small sub-group of kinases containing three members: VRK1, VRK2, and VRK3. These proteins are involved in the cell cycle, cellular proliferation, chromatin condensation and gene transcription. VRK1 has been extensively studied not only for its normal biological roles, but also for its link to cancer. VRK2 is not as well studied as VRK1. However, the two kinases are …

Self-As-Doer Identity And Health Behaviors: How Approach And Avoidant Orientation Correlate With Healthy Eating, Cooper Scheldroup, Kaitlyn Rusch, Katelyn Kelley, Abree Dieterman

Research & Creative Achievement Day

Introduction: The self-as-doer identity, an identity which describes one in correspondence with doing a behavior, has shown to improve healthy eating behaviors but less is known about how the orientation toward the doer identity (i.e., approach vs avoidant) might affect healthy eating behaviors. Approach motivation puts energy towards achieving a positive stimulus whereas avoidant motivation uses energy to avoid an aversive stimulus. Research indicates that approach goals are simpler to implement and may be better for initial behavioral enactment, while avoidance goals may be better for behavior maintenance.

Although research suggests that approach goals may be successful in behavioral change …

Synthesis And Characterization Of Cdse Nanoparticles For Use In Solar Cells, Benjamin A. Carvell

Research & Creative Achievement Day

Over the last several years, interest in thin-film, nanoparticle-based solar cells have increased due to their potential to be low cost, tunable, and highly efficient when compared to other current solar cell technologies. In this research, cadmium selenide nanoparticles capped by oleic acid (CdSe-OLA) were synthesized. After those nanoparticles were synthesized, the bulky oleic acid ligand was exchanged with much smaller, more tunable ligands such as vanillic acid, malic acid, and Sodium 2,3-Dimercaptopropane-1-sulfonate (DMPS). This allows for easier energy transfer when applied to a solar cell. The successful synthesis of these nanoparticles was confirmed using FTIR, UV-vis, and NMR spectroscopy. …

Survey Of Experiences During The Holocaust, Abraham Pasterivar

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Anonymous None

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Anonymous None

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Estelle Glaser

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Anonymous None

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Harold Spear

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Leopold Lowy

Survey of Experiences During the Holocaust

No abstract provided.

Using Natural Condiments To To Manage Diabetes Mellitus, Vignesh Tiruvannamalai '24

IMSAloquium Student Investigation Showcase

Diabetes mellitus is a predominantly chronic disease that results from blood glucose levels that exceed healthy limits. Diabetes is on the rise in modern times, and an estimated 366.000,000 people are expected to have this disease by 2030. The primary way to treat diabetes and prevent complications is to have proper dietary habits and eat nutritious foods. Some condiments are even known to help reduce blood glucose concentrations. Therefore, these condiments (turmeric, psyllium, barnyard millet, cinnamon, fenugreek, and bitter melon) were added to simulated blood at scaled amounts of 50%, 75%, and 100% of their daily recommended maximum intakes. The …

Survey For Experiences During The Holocaust, Tony Deutsch

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Abram Kniazer

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Anonymous None

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Sarah Bick-Beckhaus

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Morris Krasner

Survey of Experiences During the Holocaust

No abstract provided.

Survey Of Experiences During The Holocaust, Ben Stern

Survey of Experiences During the Holocaust

No abstract provided.

LxrTm White Clover: Development Of Transgenic White Clover (Trifolium Repens) With Delayed Leaf Senescence, Y. H. Lin, J. Chalmers, E. Ludlow, C. Pallaghy, G. E. Schrauf, Pablo Rush, A. M. García, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1977-2023)

Leaf senescence is a type of programmed cell death characterized by loss of chlorophyll, lipids, protein, and RNA. Cytokinins are a class of plant hormones that play roles in many aspects of plant growth and development, including leaf senescence, apical dominance, the formation and activity of shoot meristems, nutrient mobilization, seed germination, and pathogen responses. They also appear to mediate a number of light- regulated processes, such as de-etiolation and chloroplast differentiation. It is known that the concentrations of endogenous cytokinins decline in plant tissues as senescence progresses. This observation provides the opportunity to manipulate the senescence program in transgenic …

Production And Analysis Of Transgenic White Clover (Trifolium Repens) Plants Over-Expressing Organic Acid Biosynthetic Genes, C. M. Labandera, Stephen N. Panter, A. Winkworth, J. Simmonds, A. Mouradov, U. John, P. W. Sale, G. C. Spangenberg

IGC Proceedings (1977-2023)

Aluminium (Al) toxicity is a major environmental limitation for plant production in acid soils, which represent more than one third of the world’s agricultural land. Al-induced secretion in roots of organic acids (OA), such as malate and citrate, chelates the toxic Al cation excluding it from the root. This mechanism of Al-tolerance appears also to be associated with enhanced P-use efficiency. The development of transgenic plants for enhanced synthesis and secretion of OA from roots is a promising approach to confer Al-tolerance and enhanced P-acquisition efficiency. In order to understand the association between OA biosynthesis and secretion from roots in …

Minimising Bloat Through Development Of White Clover (T. Repens) With High Levels Of Condensed Tannins, M. T. O’Donoghue, C. Spillane, E. Guiney

IGC Proceedings (1977-2023)

White clover constitutes a low percentage of the overall sward content in Irish pastureland despite EU directives limiting the use of nitrogenous fertilizers. This is mainly due to the tendency of large amounts of white clover to cause bloat. Bloat is a potentially fatal build up of proteinaceous foam in the guts of ruminants. Some lesser cultivated legumes such as Lotus species contain condensed tannins (CT) that decrease the incidence of bloated animals. The project’s objective is to reduce the risk of bloat by generating white clover cultivars with high CT content. We are investigating whether expression of the ANTHOCYANIN …

Polyphenolic Phenomena: Transgenic Analysis Of Some Of The Factors That Regulate The Cell-Specific Accumulation Of Condensed Tannins (Proanthocyanidins) In Forage Crops, Mark P. Robbins, G. Allison, D. Bryant, P. Morris

IGC Proceedings (1977-2023)

Condensed tannins biosynthesised within crops are a well-established mechanism for protecting plant protein in the rumen of grazing livestock. Protein protection mediated by these polymeric flavonoid molecules has been characterised in Lotus spp. and offers an interesting contrast to the polyphenol oxidase (PPO) system that confers protein protection in red clover.

Molecular Breeding Of Transgenic Virus-Immune White Clover (Trifolium Repens) Cultivars, M. Emmerling, P. Chu, K. F. Smith, C. Binnion, M. Ponnampalam, P. Measham, Z. Y. Lin, N. R. Bannan, T. C. Wilkinson, G. C. Spangenberg

IGC Proceedings (1977-2023)

White clover (T. repens L.) is a major component of improved pastures throughout the temperate world. It is, however, highly susceptible to virus infection. Alfalfa mosaic virus (AMV), clover yellow vein virus (CYVV) and white clover mosaic virus (WCMV) all contribute to a significant reduction in dry matter yield and persistence of white clover. Sources of natural resistance to AMV in white clover or sexually compatible species are not available. Pathogen-derived resistance strategies, such as the expression of viral coat protein in transgenic plants, thus provides opportunities for the development of virus immune transgenic white clover.

Identification Of Quantitative Trait Loci For Flowering Time In A Field-Grown Lolium Perene X Lolium Multiflorum Mapping Population, R. N. Brown, R. E. Barker, S. E. Warnke, L. A. Brilman, M. A. R. Mian, S. C. Sim, G. Jung

IGC Proceedings (1977-2023)

Perennial ryegrass (L. perenne) and annual, or Italian, ryegrass (L. multiflorum) are considered to be separate species by the seed trade, and are used and bred for distinct purposes. However, the two species are cross-fertile. Seed producers rely on the different flowering times of the two species to produce pure seed. Flowering times can overlap, leading to genetic mixing. Contamination of perennial ryegrass seed lots with annual types is an expensive problem for grass seed producers in western Oregon, USA.

Approaches For Associating Molecular Polymorphisms With Phenotypic Traits Based On Linkage Disequilibrium In Natural Populations Of Lolium Perenne, L. Skøt, J. Humphreys, I. P. Armstead, M. O. Humphreys, J. A. Gallagher, I. D. Thomas

IGC Proceedings (1977-2023)

Association mapping relies on linkage disequilibrium (LD) between haplotypes and quantitative trait loci (QTL). The level of LD in a genome determines the resolution of this approach. In out-breeding species, LD is expected to decay rapidly, thus allowing for high-resolution mapping. It has been most extensively used in human genetics, but recent work with maize populations has demonstrated its potential in plants (Thornsberry et al., 2001; Wilson et al., 2004), and used in L. perenne to identify AFLP markers associated with a major QTL for heading date on linkage group 7 (Skøt et al., 2004). The objective …

Quantitative Trait Loci For Vegetative Traits In Perennial Ryegrass (Lolium Perenne L.), A. M. Sartie, H. S. Easton, Marty J. Faville, C. Matthew

IGC Proceedings (1977-2023)

Physiological (EP) research in forage grasses relates traits such as leaf elongation rate (LER), leaf elongation duration (LED), and leaf appearance interval (ALf), to forage yield (Chapman & Lemaire, 1993). This paper reveals preliminary quantitative trait locus (QTL) discovery for eight EP traits in perennial ryegrass. It also investigates the potential role of multivariate analyses such as principal component analysis (PCA) in QTL analysis of EP data.

Mapping Water-Soluble Carbohydrate Content In Perennial Ryegrass, L. Turner, J. Gallagher, I. P. Armstead, A. Cairns, M. O. Humphreys

IGC Proceedings (1977-2023)

Perennial ryegrass (Lolium perenne L.) is the main species used in UK agriculture and shows considerable genetic variation for water-soluble carbohydrate (WSC) content (Humphreys, 1989, Turner et al., 2001, 2002). High-sugar grasses have already proved useful in UK livestock production (Miller et al., 2001), but can be unpredictable in the field. Increased understanding of carbon partitioning in ryegrass would benefit future breeding programmes.

Changes In Gene Expression During Acclimation To Cold Temperatures In White Clover (Trifolium Repens L.), M. Lowe, R. P. Collins, M. T. Abberton

IGC Proceedings (1977-2023)

White clover is an important component of many temperate pastures and improved winter hardiness is a major objective of breeding programmes in many countries. Exposure to cold and fluctuations in temperature are components of winter stress and although some studies have investigated the agronomic and physiological mechanisms of cold tolerance, little research has been carried out to identify the genes involved. We are complementing mapping of quantitative trait loci (QTL) responsible for cold tolerance with studies of variation in gene expression between plants growing at different temperatures. In particular we are initially focusing on the process of acclimation by analysing …