Digital Commons Network^™

Identification Of Quantitative Trait Loci For Flowering Time In A Field-Grown Lolium Perene X Lolium Multiflorum Mapping Population, R. N. Brown, R. E. Barker, S. E. Warnke, L. A. Brilman, M. A. R. Mian, S. C. Sim, G. Jung

IGC Proceedings (1977-2023)

Perennial ryegrass (L. perenne) and annual, or Italian, ryegrass (L. multiflorum) are considered to be separate species by the seed trade, and are used and bred for distinct purposes. However, the two species are cross-fertile. Seed producers rely on the different flowering times of the two species to produce pure seed. Flowering times can overlap, leading to genetic mixing. Contamination of perennial ryegrass seed lots with annual types is an expensive problem for grass seed producers in western Oregon, USA.

Approaches For Associating Molecular Polymorphisms With Phenotypic Traits Based On Linkage Disequilibrium In Natural Populations Of Lolium Perenne, L. Skøt, J. Humphreys, I. P. Armstead, M. O. Humphreys, J. A. Gallagher, I. D. Thomas

IGC Proceedings (1977-2023)

Association mapping relies on linkage disequilibrium (LD) between haplotypes and quantitative trait loci (QTL). The level of LD in a genome determines the resolution of this approach. In out-breeding species, LD is expected to decay rapidly, thus allowing for high-resolution mapping. It has been most extensively used in human genetics, but recent work with maize populations has demonstrated its potential in plants (Thornsberry et al., 2001; Wilson et al., 2004), and used in L. perenne to identify AFLP markers associated with a major QTL for heading date on linkage group 7 (Skøt et al., 2004). The objective …

Quantitative Trait Loci For Vegetative Traits In Perennial Ryegrass (Lolium Perenne L.), A. M. Sartie, H. S. Easton, Marty J. Faville, C. Matthew

IGC Proceedings (1977-2023)

Physiological (EP) research in forage grasses relates traits such as leaf elongation rate (LER), leaf elongation duration (LED), and leaf appearance interval (ALf), to forage yield (Chapman & Lemaire, 1993). This paper reveals preliminary quantitative trait locus (QTL) discovery for eight EP traits in perennial ryegrass. It also investigates the potential role of multivariate analyses such as principal component analysis (PCA) in QTL analysis of EP data.

Mapping Water-Soluble Carbohydrate Content In Perennial Ryegrass, L. Turner, J. Gallagher, I. P. Armstead, A. Cairns, M. O. Humphreys

IGC Proceedings (1977-2023)

Perennial ryegrass (Lolium perenne L.) is the main species used in UK agriculture and shows considerable genetic variation for water-soluble carbohydrate (WSC) content (Humphreys, 1989, Turner et al., 2001, 2002). High-sugar grasses have already proved useful in UK livestock production (Miller et al., 2001), but can be unpredictable in the field. Increased understanding of carbon partitioning in ryegrass would benefit future breeding programmes.

Changes In Gene Expression During Acclimation To Cold Temperatures In White Clover (Trifolium Repens L.), M. Lowe, R. P. Collins, M. T. Abberton

IGC Proceedings (1977-2023)

White clover is an important component of many temperate pastures and improved winter hardiness is a major objective of breeding programmes in many countries. Exposure to cold and fluctuations in temperature are components of winter stress and although some studies have investigated the agronomic and physiological mechanisms of cold tolerance, little research has been carried out to identify the genes involved. We are complementing mapping of quantitative trait loci (QTL) responsible for cold tolerance with studies of variation in gene expression between plants growing at different temperatures. In particular we are initially focusing on the process of acclimation by analysing …

Estimation Of The Coefficient Of Double-Reduction In Autotetraploid Lucerne, R. Ayadi, P. Barre, Christian Huyghe, B. Julier

IGC Proceedings (1977-2023)

Polyploidy plays an important role in the evolution of species and many cultivated species, particularly in angiosperms, are polyploids (Bever and Felber, 1992; Gallais, 2003). Autopolyploid species that show a tetrasomic inheritance have complex genetics. However, some theoretical models were built for: (1) genetic mapping (Hackett et al., 1998), (2) quantitative genetics (Gallais, 2003) and (3) population genetics (Ronfort et al., 1998). But in practice, most data analyses ignore one essential feature of tetrasomic inheritance that is double-reduction. Indeed, in an autotetraploid species, homologous chromosomes can form tetravalents at meiosis. In this case, a double-reduction is observed if …

Development Of Est-Derived Simple Sequence Repeat (Ssr) Markers In Italian Ryegrass (Lolium Multiflorum Lam.), M. Hirata, Y. Miura, T. Takamizo, M. Fujimori

IGC Proceedings (1977-2023)

Italian ryegrass (IRG, Lolium multiflorum Lam.) is an important grass species used for agricultural purposes in Japan. Linkage maps in IRG have been constructed using molecular markers including RFLPs, AFLPs (Inoue et al., 2003) and genomic SSRs. The SSR marker is a useful tool for genome analysis because it is PCR-based with a relatively high level of polymorphism, co-dominantly expressed. The EST-SSR markers are expected to be more conserved and have a higher rate of transferability than genomic SSR markers (Scott et al., 2000), and the gene functions of ESTs may be predicted. Ikeda et al. (2004) …

Effects Of Labetolol On Sign Tracking, Cassidy Bos, Brandon Yates, Liam Kubitschek, John Holden

Research & Creative Achievement Day

Sign tracking is defined as when an organism interacts with a conditioned stimulus that signals something desired. This is comparable to when a drug addict is engaging in drug seeking behaviors after interacting with a stimulus which could be associated with drug use. Reducing this type of behavior in addicts who are trying to overcome drug dependence may help prevent relapse. One drug which could be useful is Labetalol, which is an alpha and beta-blocker that is used to treat high blood pressure (hypertension). 17 male Sprague Dawley rats were conditioned over the course of 5 days to associate the …

Influence Of Ground Reaction Forces And Joint Velocities On Kicking Velocity, Miah L. Busse, Maggie L. Plouff, Brady Ferguson

Research & Creative Achievement Day

Introduction: Kicking is a vital component in the game of soccer. One major factor that influences the success of a scoring attempt is ball velocity. Ground reaction force (GRF) and joint velocities of the lower extremities are variables of interest for increasing kicking velocity. Previous studies have shown exercise programs used to strengthen the muscles used in kicking have been successful in increasing kicking velocity (Manolopoulos, et al., 2013).[GJR1] To date, no known studies have analyzed the specific relationship between GRF, joint velocity and kicking velocity.

Purpose: The goal of this pilot study was to analyze the influence of ground …

Welding 3d Printed Structures For Composite Sacrificial Tooling, Dominic L. Perez, Kyle Gerold, Matt K. Ficker, Matthew D. Wooden, Aidan M. Dungy, Brennan G. Coit

Research & Creative Achievement Day

3D printed parts offer the ability to generate dimensioned, complex objects with minimal machining touch time and skill, but they are typically very weak and limited in size. Strong and light composite parts require tooling to be created for the fabric to lay on while the resin is curing, but tooling can be quite expensive during the prototyping phase or for low part quantity runs. This study examined the techniques required to weld smaller 3D printed parts together to form large 3D printed tools that could be used as a sacrificial tool for a composite part. In this method, the …

Investigations Of The Effects Of Mutations In Inscuteable Mediated Pins-Mud Complex And Par Complex Functions In Drosophila Neuroblast, Ryan Liu

Research & Creative Achievement Day

Drosophila melanogaster central brain neuroblasts asymmetrically segregate proteins along the cell's apical-basal axil. Divisions along abnormal orientations or the complete failure to divide asymmetrically may result in overgrowth phenotypes. Neuroblastoma, one of the deadliest solid tumor diseases with extremely poor survival rate for late stages, is an example of this dysregulation. The protein Inscuteable (Insc) was previously found to bind with the protein Partner of Inscuteable (Pins; mPins or LGN in mammals), a receptor-independent G-protein activator, which is a subunit of the Pins-Gαi complex. The combined complex recruits Dlg to ensure correct spindle attachment on the cortex of the cell, …

Feasting Or Fasting: The Influence Of Feeding Regime On Physiological Performance., Kailee Greenough

Research & Creative Achievement Day

Prior studies have shown that the environment can significantly influence the development of physiological and physical traits of life. Specifically, we investigated how the developmental condition of feeding regime influences the physiological performance of boas (Boa constrictor imperator). This study was part of a long-term study of different feeding regimes (feasting=weekly, fasting=1/5weeks). Prior studies in our lab have uncovered morphological differences in growth. These studies demonstrated that snakes fed frequently became more muscular and massive whereas snakes fed infrequently were more refined and less muscular despite being fed the same relative amount of prey. Our study added to …

Investigation Of Mitogenic Activity Of Baculovirus Fibroblast Growth Factors, Ellyssa Wager, Kassandra Hernandez, Justin Johnson

Research & Creative Achievement Day

New Title: Establishing Optimal Seeding Density and Ideal Starvation Conditions of Sf9 and NIH/3T3 Cell Lines

Fibroblast Growth Factors (FGFs) are a family of structurally related polypeptide cytokines. 22 FGF genes (FGF1-14, FGF16-23) are found in the human genome. FGFs have various known intracrine, paracrine, and endocrine functions and are essential for the development and wound repair in organisms through these mechanisms. The role of FGFs in viruses has become an area of piqued interest in the field of pathology as several virus families have genomes that encode one or more growth factor homologues. It has been suggested that a …

Carbon Cornhole, Justin O. Dailey, Blake M. Endle, Joshua J. Lebrun, Bailey J. Banaszynski, Josiah M. Gleisner

Research & Creative Achievement Day

The purpose of this design project is to create a durable lightweight yard game for players of all skill levels. Carbon Cornhole is a composite improvement on the already popular yard game, cornhole, with a new twist. The final product is based on valued feedback from customers. The board is manufactured of carbon fiber/epoxy sandwich panels with a closed cell foam core. The composite design provides a lightweight product. The panels are specifically manufactured to retain their mechanical properties when exposed to outdoor elements such as water and UV radiation. The addition of inlayed LED lights and two new "skill …

A Closer Look At The Death Penalty In The United States, Anna L. Cardell

Research & Creative Achievement Day

The death penalty has always been a source of contention towards most people in the United States – some agree with it, some do not. No matter one's opinion on whether it should be allowed, this poster presentation will give an in-depth analysis and present various visualizations of information regarding the convicted felons that have been sentenced to capital punishment in the United States from 1976-2016. Variables such as gender, age, and race will be explored to create some insight on some of the most infamous killers in United States history. Though looking at the demographics of these individuals and …

Morphological Identification Of Parasites Found In Ducks (Family Anatidae) Along The Mississippi River: A Parasitology Class Project, Taya K. Endreson, Madeline E. Volhard, Visara S. Sok, Amanda A. Amofa, Emma C. Fortsch, Delaney L. Reider

Research & Creative Achievement Day

Ducks (Anatidae) can be found across much of the United States and are hosts to a variety of parasites such as nematodes, trematodes or cestodes. This study focused on identifying the species of the parasites found within ducks based on their morphological features. The morphological structures consisted of body shape, internal organs, mouthparts, and length. The ducks used in this study were legally harvested and donated by hunters from areas across the Mississippi River in Buffalo County and Trempealeau County Wisconsin. A total of 108 ducks have been analyzed for parasites. It is important to identify the types of parasites …

Validation Of Computational Methods For The Tautomerism Of Thiourea Dioxide, Mckenna Lund

Research & Creative Achievement Day

In solution, thiourea dioxide exists in an equilibrium between its dioxide and sulfinic acid forms. This tautomeric relationship, as they all are, is substantially influenced by the solvent's polarity and hydrogen bonding capabilities. More well-known systems, e.g., acetylacetone, have been extensively studied in this regard, while thiourea dioxide's equilibrium has not. We have initiated a hybrid computational-spectroscopic investigation of this system utilizing low-cost Hartree-Fock methods with the 3–21G, 3–21+G, 3–21G*, 3–21+G*, MINI, MIDI, SBKJC and HW basis sets. Beyond gas phase calculations, both tautomeric forms were modeled using the C-PCM implicit solvent model for water and toluene. Obtained modeling results …

Flexural Creep Comparison To Tts Creep For Pp And Pp-Gf30, Max R. Kauphusman, Regan G. Harvey

Research & Creative Achievement Day

Creep testing is one of the most expensive datasets to acquire in both cost ($10k+) and time (2+ months). Dynamic Mechanical Analysis offers a unique opportunity to characterize creep properties of polymers using a technique called Time Temperature Super Positioning (TTSP) in a relatively short period of time. TTSP works by holding either frequency or loading constant at set temperatures for a constant period of time. WLF equations are then used to combine multiple curves from different temperatures into a master curve that can be used to extrapolate out creep performance at designated reference temperatures and save a considerable amount …

Vfgf Protein Purification, Expression, And Proliferation Assay, Justin Johnson, Kassandra Hernandez, Ellyssa Wager

Research & Creative Achievement Day

Fibroblast growth factors (FGF's) are signaling molecules (cytokines) secreted from specific cells known as fibroblasts. They are essential for embryonic development, function postnatally in response to injury, in regulation of electrical excitability of cells, and as hormones that have a role in regulating metabolism. Viral Fibroblast Growth factors Ac6 and Cf6 have been encoded into E. coli plasmids which produce high concentrations of our target vFGFs which are then purified for testing. The vFGFs were expressed with maltose binding protein (MBP) tagged on the N-termini. MBP is used as a pseudo-chaperone with a hydrophobic cleft that can refold the protein …

Comparison Of Colorimetric Assays To Use For The Investigation Of The Mitogenic Activity Of Vfgf In Cell Cultures, Kassandra Hernandez, Ellyssa N. Wager, Justin Johnson

Research & Creative Achievement Day

Fibroblast growth factors (FGFs) are part of a large family of polypeptide growth factors that play a large role in the development and regulation of organisms using intracrine, paracrine, and endocrine cell signaling mechanisms. FGFs are known to be found in both vertebrate and invertebrate organisms. Most recently they have also been identified in viruses, such as baculoviruses. A sequence analysis of the baculovirus genomes has found that they encode for a viral fibroblast growth factor homolog (vfgf) early on in their stage of infection (Katsuma et al. 2006). Although there have been studies from other labs that …

Changes In Depression, Stress, And Anxiety For Student Athletes: Using Guided Imagery, Synetha K. Sultze-Lor

Research & Creative Achievement Day

Introduction: Mental health concerns among college students are increasing in severity. Researchers have shown that elite level athletes may be at a higher risk for suicide, with possible risks including injury, pressure, and substance abuse. Due to the high-stress levels of student athletes, this study was created to explore whether guided imagery (GI) can help student athletes reduce symptoms of stress, depression, and anxiety.GI has been shown to decrease depression and anxiety among a variety of populations, but less work has explored the role that GI has in student athletes. Therefore, it was hypothesized that GI will reduce stress, and …

Improvement Of Native Perennial Forage Plants For Sustainability Of Mediterranean Farming Systems, F. Lelièvre, Florence Volaire

IGC Proceedings (1977-2023)

The amount of water available to agriculture in the Mediterranean is declining because of increasing population pressure and greater incidence of drought. Therefore, the efficiency of the use of water for agricultural production must be maximized and, in this context, perennial forage species have a number of advantages in comparison to the predominantly-used annuals. They can utilize water throughout the whole year besides being able to halt rangeland degradation, restore soil fertility and enhance forage production, thereby contributing to greater sustainability of rain-fed agricultural systems in the southern European Union and North Africa. Despite these advantages, the small size of …

The Potential For Summer-Dormant Perennial Grasses In Mediterranean And Semi-Arid Pastures, F. Lelièvre, Florence Volaire, P. Chapon, M. Norton

IGC Proceedings (1977-2023)

In rain-fed Mediterranean and semi-arid areas, herbage production of perennial grasses depends on their ability to grow efficiently during the rainy seasons and to persist over the dry summer. A key survival strategy in these harsh conditions is summer dormancy (Volaire, 2002). Within the species Dactylis glomerata L., two cultivars (cvs.), contrasting in this trait, were compared in order to analyse their suitability in terms of yield and survival in these environments.

Analysis Of Efficiency Of Dark Photon Decay To Lepton Jets, Sree Atyam '24, Malcolm Wilson-Ahlstrom '24

IMSAloquium Student Investigation Showcase

Dark photons are useful indicators to explain important phenomena beyond the Standard Model of particle physics, namely in the context of many experiments: dark matter. Dark photons can interact with Standard Model particles through a process called kinetic mixing, which allows them to decay into Standard Model leptons, from which lepton jets are produced. The area of interest in these jets comes in the form of analysis of the varying-flavor pairs in the jets. If the given pair in a lepton jet has two particles of different flavor, this observation can offer evidence for possible connections to show relative probabilities …

Digital Commons And Pathways To Publication, Raven Mckelvin '24

IMSAloquium Student Investigation Showcase

In this internship, student Raven McKelvin has been working on Identifying and Strengthening Existing Support for Students on a Research-to-Publication Pathway through portfolio building, workshops, and presentations. While also working with Jean Bigger on advancing information about DigitalCommons.

Information Scoping Skills And The Research Output Of Academic Librarians In Universities In South-South Geopolitical Zone Of Nigeria, Godson Ehimiyein Ilevbare Phd, Uduak Ubong Enang Phd, Mercy Etukudo Ukpanah Phd

Library Philosophy and Practice (e-journal)

This study investigated the relationship between information scoping skills and the research output of academic librarians in universities in South-South Geopolitical Zone of Nigeria with corresponding specific objective, research question, and research hypothesis. To effectively drive the study two theories were adopted. The theory of social cultural learning for the independent variable (information scoping skill), and the theory of self-efficacy for the dependent variable (research output of academic librarians). More so, conceptual review of literature on information scoping skills and research out were carried, and specific empirical review of literature with extrinsic similarity and dissimilarity to the study was also …

Breaking A Bad Stigma: Breaking Bad And The Need For Dynamic Fatherhood Roles To Overrule Historical Stereotypes, Ella Bellflower

Line by Line: A Journal of Beginning Student Writing

Editor's note: This paper is the recipient of the Best-Researched Argument award.

The overarching theme of my English 114 course focused on the depiction of family in popular culture. Thus, the final research project required me to research some aspect of family in American or popular culture, which led to my decision to analyze the 2008 television series Breaking Bad. My initial research question sought to answer how fatherhood was depicted in the show. However, I was unable to find much research on the show in general, leading me to choose a more physical and psychological route. I came …

Maimonides And Julian: A Guide To Love, Alexandra Amrhein

Line by Line: A Journal of Beginning Student Writing

For this project, the goal was to write an explanatory analysis of Maimonides’ Guide of the Perplexed, reconstructing his case on divine revelation and intellectual perception. We were asked to then compare Maimonides’ themes to those represented in Julian of Norwich’s Revelations of Divine Love. I began by closely rereading the assigned section of the Guide’s introductory chapter and taking some notes on the main ideas Maimonides presents. Afterwards, I drafted the body of the essay, summarizing these themes and supporting the summary with specific textual evidence. I then reread Julian’s Revelations, brainstormed major similarities and differences between …

The Truth Untold: The Compromised Election Of 1876, Julie Pugh

Line by Line: A Journal of Beginning Student Writing

My writing process always begins with a slow start. The first steps of this historiography were spread over a long period of time, with a majority of the essay being written within a couple of weeks. I begin by choosing a topic for my paper which I selected from a list of options collected during the preliminary research period. Once I decided on the Election of 1876 and the Compromise of 1877, I drafted a topic proposal and a list of sixteen potential research sources from online databases and the library. From the potential sources, I narrowed my research to …

Another Brick In The Wall: A Historiography Of Black Education In The Reconstruction Era, Jane Carney

Line by Line: A Journal of Beginning Student Writing

This project involved several intermediate assignments, including a topic proposal and annotated bibliography. I also submitted a draft to my professor for review and attended a peer review session in class.