Digital Commons Network^™

The 3-D Apparatus Of Local Ticks: A New Insight Into The Mechanism Of Sensing Hosts And Their Storage Of Pathogens, Sara Grant, Tristan Davis, Maggie Milhorne, Zipporah Nedlock, Jonathan Marks, Alan L. Gillen

Liberty University Research Week

No abstract provided.

Production, Isolation And Characterization Of Novel Peroxidases From Cucurbita Family, Rahel Mamo

Liberty University Research Week

Peroxidases are found throughout nature and are valuable biocatalysts due to their versatility in catalyzing oxidative reactions using hydrogen peroxide as a co-substrate. The peroxidase from horseradish root is widely used in diagnostics and synthetic applications, however, plant peroxidases from other plant sources remain underexplored. Recently, a peroxidase from the skin of Cucurbita maxima (PKS) was isolated and demonstrated promising biocatalytic properties related to the decomposition of a wide variety of fluorinated aromatic compounds. Purification and characterization of this enzyme revealed a protein sequence that was similar to the well characterized HRP, with some key amino acid substitutions. A second …

Investigating The Natural Communities And Flora Of Candler’S Mountain, Lydia Harris, Cyrus Harris, Tyler Knick

Liberty University Research Week

Candler’s Mountain is home to a variety of natural communities and flora. With more than 1200 vascular plant species documented in Campbell County, the identification and growing historical record of local flora will support short and long-term recognition of the importance of biodiverse natural communities in central Virginia. This project seeks to (1) document flora diversity in this region, (2) further establish an herbarium teaching and research collection of representative species, and (3) advance our understanding of the flora in relation to natural communities. Identified ecological groups and community types are based upon the Virginia Department of Conservation and Recreation …

Characterizing B1 B-Cells In Zebrafish (Danio Rerio), Alyssa Canelo, Victoria Pacheco

Liberty University Research Week

This project tests the hypothesis that developing antibodies specific to zebrafish CD5 and IgM will enable the reliable identification and characterization of B1 B-cells in zebrafish. B1 B-cells play a critical role in autoimmunity across species, yet their functions remain poorly characterized in zebrafish (Danio rerio). Zebrafish are widely used as a developmental and immunological model organism due to their genetic similarity to humans as well as their transparent embryos. The lack of species-specific antibodies has hindered the study of B1 B-cells in zebrafish.

This project seeks to develop and validate antibodies against zebrafish CD5 and IgM. This will be …

Kinetic Analysis Of Degradation And Maximum Catalytic Capacity For Plant Peroxidase Enzymes, Hayden Stollings

Liberty University Research Week

In 2006, the Environmental Protection Agency (EPA) began investigation into the “forever chemicals” known as PFAS. PFAS, which stand for “per- and polyfluoroalkyl substances,” are a highly stable and toxic compound that were mass produced throughout the 1900’s and was commonly found in common kitchenware, carpets, furniture and even produced in industrial processes.

The function behind PFAS’ stability lies in the abnormally strong Carbon-Fluoride bond, which explains for its initial functionality. Moreso, PFAS can withstand natural degradation in the environment, leading to buildup. Exposure to certain PFAS have been linked to health problems such as increased cholesterol, thyroid issues, reproductive …

Characterizing Novel Peroxidase Activity Through Enzyme Kinetics And Equilibrium Binding, Marika Yelle

Liberty University Research Week

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants, motivating the exploration of peroxidases as potential bioremediation catalysts. This study spectroscopically characterized three peroxidases, with a focus on Pumpkin Skin Peroxidase (PKS). Initial characterization assessed PKS equilibrium binding with various ligands to determine dissociation constants (Kd) for each ligand-enzyme interaction. To characterize PKS catalytic activity, guaiacol oxidation was monitored, with parallel KF trials modelled by Michaelis-Menten kinetics, revealing fluoride-induced increases in Km and Vmax, consistent with an inhibitory effect on substrate binding and catalytic turnover. These findings provide insight into the function of PKS and its potential for environmentally relevant …

Investigating The Role Of The Active-Site Metal In Plant Peroxidase Catalysis, Alexandra Knight

Liberty University Research Week

This study investigates how replacing the native iron heme with manganese or cobalt protoporphyrins affects the enzymatic activity of horseradish (HRP), pumpkin skin (PKS), and butternut squash (BNT) peroxidases. A heme extraction protocol was adapted for generating apoenzymes by removing the native iron heme cofactor, and extraction was confirmed using UV-vis spectroscopy. While initial reconstitution of apo-HRP with manganese protoporphyrin IX appeared to be incomplete based on UV-vis analysis, the results suggested that manganese incorporation modifies the spectroscopic characteristics of the native enzyme. Comparing horseradish, pumpkin skin, and butternut peroxidases will reveal potential differences in metal-dependent activity and stability among …

Morphological Evaluation Of Cadaveric Tissues Integrity Using Histology Staining, Jumana Rizk, Levon Shukrian, Maria Mendoza Lopez, Shanice Rerrie

Liberty University Research Week

The transition from gross anatomical dissection to microscopic evaluation is essential for a comprehensive understanding of human biology. While systemic anatomy reveals the macroscopic organization of the body, histology provides insight into cellular structure and tissue integrity. This study aimed to bridge these disciplines through histological assessment of cadaveric tissues. Our objective was to determine whether tissue samples after prolonged fixation could be processed and stained to meet standards for histology education. Tissue samples of the cerebral cortex, renal cortex, liver lobe, and skeletal muscle were obtained from two cadaveric donors, one male and one female. Tissues were processed, embedded …

“Slaves To Sin:” Harnessing Neuroplasticity To Defeat Habitual Behaviors, Charity Parrott

Liberty University Research Week

How does neuroplasticity affect the life of a Christian? This thesis will seek to address the concepts of habit formation, neuroplasticity, and top-down control through a neurobiological perspective, and how they relate to the Biblical concept of habitual sin. Discoveries in neuroplasticity have revealed the brain to be a malleable organ, capable of rewiring neural connections and, in the hippocampus region, neurogenesis, or the creation of new neurons (Fuchs & Flügge, 2014). This thesis will compile research in neuroplasticity, explore the biological reasons behind the difficulty in escaping patterns of sin, and connect Biblical-based strategies for sin prevention with neurobiology. …

Playing The Past: Girls, Dolls, And Entrepreneurs, Taylor Schmalz

Liberty University Research Week

Mattel, Inc. is a toy company located in California and sells some of the most iconic children’s toys across gender lines. From Hot Wheels, Barbie, Fisher-Price, and over a hundred more toy brands, Mattel, Inc. has touched millions of lives since its creation in 1945. Two of the most famous being American Girl Dolls and the Barbie Doll. Each doll was created by female entrepreneurs and was made to empower little girls in America. While the dolls were created to empower girls, teach feminist ideas, and share women’s history, the dolls face backlash for not being feminist enough, too grown …

Effect Of In-Utero Exposure To A Western-Style Diet On Testis Development, Alexa Ashby, Colton Fitzgerald, Kaylee Vis, Madison Ashby, Marisa Halsey, Nathan Malina, Ryan Anderson

Liberty University Research Week

The increase in western-style dietary patterns has led to an increase in obesity prevalence and its associated comorbidities. Specifically, researchers have found that obesity due to western-style diet (WSD) is negatively correlated with reproductive health in men. This study investigated the effects of inutero exposure to a WSD on testis development, specifically of 12- week-old mice (young adult C57Bl/6J). We hypothesized that in-utero exposure to a WSD would negatively impact testis development and function in male offspring. Using histological hematoxylin and eosin staining and immunohistochemistry method, we examined spermatogenesis in seminiferous tubules, anti-Mullerian hormone (AMH) production by Sertoli cells, and …

How Did Maternal Influenza And Tdap Vaccination Coverage Among Pregnant Women In Mississippi Change From 2018–2021, And How Do Coverage Rates Differ By Race/Ethnicity?, Soala Esther Abere, Atika Ali, Fadzai Nyarugwe, Ifeoluwanimi Shobayo, Jalena Smith

Liberty University Research Week

Maternal vaccination is a critical component of preventive care, yet coverage in the United States remains inconsistent, with persistent racial and age-related disparities. This study examined maternal influenza and Tdap vaccination coverage among pregnant women in Mississippi from 2018–2021, a state with disproportionately high maternal and infant mortality. Using publicly available CDC surveillance data, a quantitative secondary data analysis was conducted to assess vaccination trends and differences by race/ethnicity and maternal age group. After excluding suppressed estimates, 48 observations were analyzed. Overall maternal vaccination coverage averaged 51.55% across all years and demographic groups, with influenza coverage ranging from 49–54% and …

Characterization Of Peroxidase From Butternut Squash Skin: Potential Use In Bioremediation, Ava Witkowski

Liberty University Research Week

Peroxidases are present in many diverse plant species and catalyze chemical oxidations, some that may have significance in biotechnological areas like bioremediation. In several recent papers we characterizes a novel peroxidase from the skin of pumpkin that possessed desirable catalytic activities in relation to a class of environmental contaminants call fluorinated phenolics. In the course of these studies, we were able to do genomic analysis on a related plant species curcubita moscata (butternut squash) and identified a peroxidase with very similar sequence. The current study focusses on the characterization of that novel peroxidase in comparison with the pumpkin skin squash. …

Sem-Edx Analysis Of Animal Hairs For The Presence Of Metals, Sydney Stevens, David Green, George Monroe

Liberty University Research Week

No abstract provided.

Analysis Of The Effects Of Phytocannabinoids Cannabichromene (Cbc), Cannabidiol (Cbd), And Cannabigerol (Cbg) On The Enzyme Faah, Rachel Duncan

Liberty University Research Week

No abstract provided.

Differential Effects Of Curcumin And C646 On Mitogenic And Metabolic Signaling In Insulin-Stimulated Hepg2 Cells, Davis Raz, Rachael De Klerk

Liberty University Research Week

Curcumin and C646 both inhibit p300/CBP histone acetyltransferase (HAT) activity, but they differ markedly in their mechanisms, potency, and effects on cellular function. Both compounds have been investigated as modulators of the insulin-signaling pathway and have shown potential for improving insulin-signaling–related defects in experimental models. Our previous findings showed that curcumin positively impacted a metabolic pathway by reducing IRS-1 phosphorylation at S1101, a negative regulatory site, while maintaining downstream PRAS40 and RPS6 phosphorylation. Although C646 also reduced IRS-1 S1101 phosphorylation, it did not preserve downstream insulin signaling as effectively as curcumin. We hypothesized that target proteins within the mitogenic pathway, …

Evaluating Sexual Dimorphism In An Urban Neonate Regina Septemvittata Population, Arleigh Wagoner, Nathan Wilkins

Liberty University Research Week

Regina septemvittata, commonly known as Queensnakes, are broadly distributed across eastern and central North America. In adult Queensnakes, females average a greater snout-vent length (SVL) than males. Since Queensnakes exhibit size dimorphism and have short life spans and low survival rates, it was hypothesized that neonate Queensnakes begin to display a distinct sex-related difference in SVL within their first few months of life. Our study investigated growth rates of neonate Queensnakes by conducting a capture-mark-recapture (CMR) study over a three-month period (August through October; birth to first brumation) in an urban stream in Lynchburg, VA. Captured snakes were measured (SVL), …

Quantifying Neuropathy With Von Frey Filaments In Mice: Novel Neuropathic Pain Animal Model, Maggie Milhorne

Liberty University Research Week

Neuropathic pain is a chronic pain condition resulting from damage to the nervous system which does not respond to current pharmacological treatments. Previous research in rats has demonstrated that formalin injections can induce chronic pain states and molecular changes within the spinal cord comparable to those of the spinal nerve ligation (SNL), an established surgical model of neuropathic pain. This study aimed to determine whether a subcutaneous injection of 5% formalin is sufficient to induce chronic pain in mice, providing a simple and less invasive model to study chronic pain mechanisms. Mechanical pain sensitivity is assessed using the von Frey …

Not Allowed To Say Goodbye: The German Civilian's Perception Of Aktion T4, 1939 - 1941, Dylan Cumbo

Liberty University Research Week

Despite the public pushback during the sterilization program, little stopped it from continuing. Nazi officials had desired for the program to expand during the early stages of sterilization, their chance coming with the death of a child and the outbreak of World War II. Six centers were designated as the main euthanasia centers along with several other locations used to support it. Gas, lethal injections, and starvations were used to kill the disabled on mass. Despite attempts to keep the program secret, they failed as clues became increasingly apparent. Documented civilian responses were largely antagonistic and actions committed were passive …

Authentic Vs. Artificial Intelligence: The Effect Of Generative Ai On Users’ Brains, Ivy Rath

Liberty University Research Week

Currently, research on generative AI's effect on the brains of those who use it is sparse. However, cognitive offloading, or the outsourcing of intellectual processes to technology or other people, is a well-known neurological phenomenon, and it is through cognitive offloading that we may begin to understand how generative AI affects mental processes. Cognitive offloading occurs frequently, and common examples include using a calculator, GPS, or driver-assist functions. Now, generative AI offers users a far more pervasive kind of cognitive offloading, in that entire mental processes once unique to human minds (such as problem-solving) may now be replicated and supplanted …

The Impact Of Maternal Western-Style Diet On Ovarian Anti-Müllerian Hormone Expression And Localization In Mouse Offspring, Kati Platt, Chase Shinabery, Jackson Mellish, Madison Howard, Soriya Scott

Liberty University Research Week

The Western diet, characterized by high saturated fat and sucrose intake, has become increasingly prevalent in the United States and globally, yet its effects on female reproductive health across life stages remain insufficiently understood. In adult human females, consumption of a Western-style diet has been linked to an increase in premenstrual symptoms, higher rates of depression, and elevated free estradiol levels, contributing to an increased risk of breast cancer, particularly in postmenopausal women. In human female children, Western diet consumption has been linked to an early onset of secondary sexual characteristics and premature menarche. Maternal consumption of this diet during …

Spectroscopic Characterization Of Pfas Ligands Binding To Novel Plant Peroxidases, Vladimir Bogatov

Liberty University Research Week

No abstract provided.

Elephant's Toothpaste: A Statistical Investigation Of The Yeast-Catalyzed Decomposition Of Hydrogen Peroxide, Annaliese Defelice

Liberty University Research Week

Catalase is the main enzyme in yeast that catalyzes the popular “elephant’s toothpaste” reaction. Elephant’s toothpaste reactions often proceed using an iodide or yeast catalyst, but iodine is favored for ease of quantitative analysis. There remains little statistical data on the yeast-catalyzed hydrogen decomposition reaction. The relationship between reagent amounts and reaction rate was measured by manipulating reagent quantity while maintaining other variables. The results were graphed and fitted for linearity, indicating the relative relationship between each reagent and the overall reaction rate. There were strong positive correlations between yeast and hydrogen peroxide concentrations and reaction rate. Augmenting the volume …

Investigating Nutritionally Derived Treatments For Metabolic Disease: Enterolactone And Its Effect On Glp-1 And Pyy Secretion, Aidan Clancy

Liberty University Research Week

Gut-derived hormones such as glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) play essential roles in maintaining glucose homeostasis and appetite regulation. These hormones are primarily secreted by enteroendocrine L cells in response to nutrient signaling in the gut. Emerging evidence suggests that polyphenolic compounds and their metabolites may influence gut hormone secretion. Enterolactone (ENL), a microbial metabolite of the dietary lignan, matairesinol, is of particular interest due to its reported anti-diabetic properties. In this study, we propose to test whether ENL can modulate the secretion of GLP-1 and PYY in L cells in vitro, thereby identifying a potential mechanism by …

Offspring Cognitive And Neurobiological Outcomes Influenced By Maternal Dietary Patterns, Emma Whatley, Abigail Frommack, Karina Long, Sarah Nelson

Liberty University Research Week

The impact of maternal diet on offspring neurodevelopment is a relatively new field, and its areas of focus are vast. The field is predominantly studied and understood through the impacts of an unhealthy diet, the maternal Western diet (WD), but there is very little research on healthy diets like the maternal Mediterranean diet (MD). The general healthiness of the diet and some limited research support that this diet could have beneficial impacts on offspring. This research aims to explore the impacts of a maternal MD on adult offspring. We hypothesize that the maternal MD will enhancing cognitive performance in learning, …

Bacterial Purification Of Hat Enzymes From Cryptococcus Neoformans: Esa1 And Gcn5 Purification, Manasi Joshi, Andrew Wisley

Liberty University Research Week

Gene expression in eukaryotes is tightly regulated by post-translational modifications. Histone acetylation is an epigenetic modification associated with increased transcription. The putative histone acetyltransferase (HAT) in the fungus Cryptococcus neoformans, Something About Silencing 3 (SAS3), has been a novel research target due to its potential role in virulence that causes nearly 112,000 to 625,000 annual deaths worldwide through cryptococcal meningitis. Although SAS3 homologs acetylate core histone 3 in mammalian organisms, recombinant SAS3 has not yet demonstrated HAT activity. Previously, an established human HAT p300 was used as a positive control to validate enzymatic conditions for HAT assays. However, using known …

Effects Of Environmental Xenoestrogens On Immune System Development In Mus Musculus, Broderick Adams, Annaliese Defelice

Liberty University Research Week

Prenatal exposure to environmental xenoestrogens may influence immune system development and contribute to autoimmune disease risk. This study investigates whether Bisphenol-C (BPC), a common substitute for Bisphenol-A (BPA), exhibits similar estrogenic and immunomodulatory effects, and whether it can cross the placental barrier. Using a controlled murine model, dams are assigned to multiple treatment groups receiving estradiol, BPA, or BPC via defined administration methods. Offspring are analyzed at gestational day 17, with collection of amniotic fluid, fetal tissues, and serum. Immune markers, including IL-6 and anti-dsDNA antibodies, are quantified ELISA and flow cytometry, while mass spectrometry is used to confirm BPC …

Impacts Of Maternal Diet On Offspring Affective Behavior And Brain Morphology, Abigail Frommack, Emma Whatley, Karina Long, Sarah Nelson

Liberty University Research Week

The field of maternal dietary impacts on offspring neurodevelopment has been established over the past 30 years, and since its establishment it has grown rapidly. The research shows that maternal diet significantly impacts offspring neurodevelopment, with those effects lasting until adulthood. Most research in this field focuses on the detrimental impacts of a Western Diet (WD), but little research exists on the beneficial impacts of a healthy diet. This research aims to close that gap by assessing a healthy diet and its impact on adult offspring. We hypothesize that a maternal Mediterranean diet (MD) will have positive impacts on the …

Enzyme Kinetics Of Faah, Susanna Stell, Emma Hooks

Liberty University Research Week

Anandamide (AEA) is an endogenous ethanolamide that functions as a signaling molecule within the endocannabinoid system, a regulatory network processes including pain perception, mood regulation, appetite, and neural signaling. AEA belongs to a broader class of fatty acid ethanolamides naturally present in the body, which also includes palmitoylethanolamide (PEA) and oleoylethanolamide (OEA). The activity of AEA is tightly regulated through enzymatic degradation by fatty acid amide hydrolase (FAAH), which hydrolyzes AEA into arachidonic acid and ethanolamine, thereby terminating its signaling activity. The purpose of this study is to investigate the rate at which AEA is hydrolyzed by the FAAH enzyme …

Pilot Study: Black Tea Extract And Theaflavin Polyphenols Effects On L6 Myotubes, Elizabeth Allen

Liberty University Research Week

This research investigates bioactive polyphenol group theaflavins found in black tea and how they affect Akt signaling in skeletal muscle. The black tea polyphenols are a collection of innate compounds in Camillia sinensis, among them being the theaflavin group which is comprised of four compounds: theaflavin (TF1), theaflavin 3-gallate (TF2A), theaflavin 3’-gallate (TF2B), and theaflavin 3,3’ digallate (TF3) (He, 2017). The theaflavins group has been described as bioactive in a variety of different cellular pathways such as apoptosis, cellular division, gene expression and nutrient regulation (Li et al., 2021)(He, 2017)(Lin et al., 2007).

Previous work by Nagano et al., demonstrated …