Digital Commons Network^™

Mechanistic Insights Of Enterolactone Within 3t3-L1 Adipocyte Cells, Merritt Smith

Liberty University Research Week

Type 2 diabetes mellitus (T2D) is one of the leading causes of morbidity and mortality in the United States, with approximately 25% of healthcare expenditures directed toward its management and associated metabolic complications. In response to the urgent need for novel therapeutic strategies, recent research has focused on bioactive compounds derived from dietary sources. In light of that, we identified the plant lignan matairesinol (MA) and its gut-derived metabolite, enterolactone (ENL), as agents capable of enhancing glucose uptake in 3T3-L1 differentiated adipocytes. These results suggest that enterolactone may represent a promising dietary supplement for improving glycemic control in patients with …

An Evaluation Of White-Spotted Bamboo Shark (Chiloscyllium Plagiosum) Development, Madison Ashby, Alexa Ashby, Baylis Huckabay, Cyrus Harris, Emma Bron, Lydia Harris, Maia Dieken, Oliver Thomas, Will Chelberg

Liberty University Research Week

An inhabitant of the north Indo-Pacific Ocean, the whitespotted bamboo shark (Chiloscyllium plagiosum) is a demersal, ambush predator of fish and invertebrates. Despite the known abundance of C. plagiosum, there is limited diagnostic information on their growth beyond embryonic development and hatching. A population of this species under managed care provides a unique opportunity to chart growth from the respective stages of life such as egg hatchling, sub-adult, and adult life stages. Through this research, we set out to chart the growth parameters on three hatchling bamboo sharks from a weekly data collection. These parameters include weight (g), sex, pre-caudal …

Personality Correlations, Blake Stillman

Liberty University Research Week

For this research project, the focus was to conduct a survey of people and ask them what their Enneagram types were, what their Myers-Briggs personalities were, and how they correlated with one another. With that information, we were then going to see what their preferred communication style was and see if there was any kind of pattern, and then apply this to the world of communication to better understand how to communicate with different personalities of people. There have not been any studies done on this specific topic and how it relates to the field of communication. I wanted to …

Evaluation Of Cadaver Tissue Antigenicity As A Practical Teaching Tool For Immunohistochemistry, Shanice Rerrie, Jumana Rizk, Levon Shukrian, Maria Mendoza Lopez

Liberty University Research Week

Immunohistochemistry (IHC) is a core technique in biomedical research and is increasingly important for teaching at the undergraduate and graduate levels. However, teaching laboratories typically rely on animal-derived tissues because the access to fresh human specimens is restricted due to ethical concerns. The purpose of this study was to evaluate whether cadaveric tissue can retain sufficient antigenicity for immunohistochemical analysis and thereby serve as an effective and ethical teaching resource. Liver lobe and renal cortex samples (approximately 1 cm³) were collected from an 88-year-old female donor. Target proteins were selected for their established roles in vitamin D metabolism and their …

When The Music Stops: How Removing The Musical Scores Changes The Message Of The Film, Natalie Dean, Caroline Breen

Liberty University Research Week

Music has always been an intricate part of film. Films like Jaws, Jurassic Park, Star Wars, Harry Potter, Avengers are not simply recognized by their logos and characters, but also popular scores and theme songs that are tailored specifically to those films. Character theme songs, opening musical scores and more are used to create a communicative connection between the film and the audience. Previous research has primarily evaluated cognitive data such as attention level, or explored altering the music itself, such as a change in tempo, rhythm, and pitch. This study investigates how a film’s overall messaging to its audience …

Single Step Cyanoacrylate Fumigation With Common Dyes, Abigail Proffitt, Jared Filegar

Liberty University Research Week

Cyanoacrylate fumigation is a widely used fingerprint development method to visualize latent fingerprints left on nonporous surfaces. This methodology relies on heating cyanoacrylate, otherwise known as superglue, to create vapors that polymerize onto fingerprints. These vapors react via biological components such as amino acids and other residues left from finger oils. This technique is very effective; however, once the polymer has completed its reaction the fingerprint is colorless resulting in the need for a secondary staining technique. The additional procedure increases processing time and introduces potentials for smearing or human error. This research aims to investigate a way to consolidate …

A Bloody Window To The Past: Str Analysis Of Dna From A Bloodstained Civil War Surgical Table, Nadia Walter

Liberty University Research Week

This study aimed to generate DNA profiles from the bloody Hillsman House surgical table via STR analysis while also determining the best method of sample collection and analysis from a wooden substrate. During the Battle at Sailor’s Creek in 1865, the historical Hillsman House served as a field hospital where hundreds of Civil War soldiers were treated. In 2016, Jones and McClintock collected and analyzed samples from bloodstains on the floorboards of the house, showing that historical DNA is still viable for profiling. During this process, marks on the floorboard were presumed to be from the surgical table, which was …

Formation Of Rosette-Like Hierarchical Structures On Cylindrical Titanium Surfaces., Cole Urquhart, Ally Doud, Michael Korn, Hector Medina

Liberty University Research Week

The purpose of this experiment was to characterize titanium-based rosettes and other micro-sized crystal formations on multiple different non-planar titanium surfaces. Rosettes are crystal structures in the titanium sulfate family. They are 20-150µm in diameter and vastly increase the surface area of the places where they are grown, which can lead to various possible applications in future research. The titanium type that was being experimented on was titanium gauze (50µm diameter). To grow titanium-based structures, two steps are involved: etching and crystal formation. Firstly, the titanium material was placed in a centrifuge tube filled with 1mL of 18M (96%) conc. …

Regulation Of Glut5 Expression And Fructose Uptake By Heat Stress And Pro-Inflammatory Cytokines In Enterocytes, Landri Shope, Gretchka Mair

Liberty University Research Week

Diabetes is an escalating global health concern, affecting 11.1% of the adult population worldwide, with over 90% of cases classified as type 2 diabetes mellitus (T2DM) (Facts & Figures., n.d.). T2DM is characterized by chronic fasting hyperglycemia and is increasingly associated with alterations in the gut microbiome. This study tests the hypothesis that heat stress, a gut dysbiosis-associated stressor, will upregulate the fructose transporter GLUT5 in enterocytes. Increased GLUT5 expression may enhance intestinal fructose uptake, thereby contributing to worsened glycemic control in T2DM. While the mechanism linking inflammation and impaired glucose homeostasis remains elusive, emerging evidence supports strong associations among …

Various Animal Species Microscopic Hair Analysis, Bryanna Smith

Liberty University Research Week

This research aims to determine whether animal hair characteristics can reliably differentiate various animal species, a study with potential forensic applications. Both human hair and animal hair are comprised of three key components: the cuticle (the outer coating composed of overlapping scales), the cortex (protein-rich structure that surrounds the medulla and contains pigments) and the medulla (the central core, which may be absent). The goal of this research project is to document data collected from microscopic analysis of a variety of non-domesticated animal hair/fur samples, such as bear, skunk, or wolf, to determine detailed similar and different characteristics between them …

Forensic Differentiation Of Canine Hair, Allison Kelley

Liberty University Research Week

The goal of this research is to determine whether the characteristics of dog hair can effectively differentiate between canine breeds, which is important for forensic applications. While there is extensive literature on human hair analysis, there is not much to be found on canine hair, although dogs are present at many crime scenes. This research ultimately aims to gather sufficient data on a wide range of dog breeds to compile a comprehensive database to identify differences in dog hair, contributing to the discipline of trace evidence hair analysis within forensic science. The central hypothesis in this study is that specific …

The 3-D Apparatus Of Local Ticks: A New Insight Into The Mechanism Of Sensing Hosts And Their Storage Of Pathogens, Sara Grant, Tristan Davis, Maggie Milhorne, Zipporah Nedlock, Jonathan Marks, Alan L. Gillen

Liberty University Research Week

No abstract provided.

Production, Isolation And Characterization Of Novel Peroxidases From Cucurbita Family, Rahel Mamo

Liberty University Research Week

Peroxidases are found throughout nature and are valuable biocatalysts due to their versatility in catalyzing oxidative reactions using hydrogen peroxide as a co-substrate. The peroxidase from horseradish root is widely used in diagnostics and synthetic applications, however, plant peroxidases from other plant sources remain underexplored. Recently, a peroxidase from the skin of Cucurbita maxima (PKS) was isolated and demonstrated promising biocatalytic properties related to the decomposition of a wide variety of fluorinated aromatic compounds. Purification and characterization of this enzyme revealed a protein sequence that was similar to the well characterized HRP, with some key amino acid substitutions. A second …

Investigating The Natural Communities And Flora Of Candler’S Mountain, Lydia Harris, Cyrus Harris, Tyler Knick

Liberty University Research Week

Candler’s Mountain is home to a variety of natural communities and flora. With more than 1200 vascular plant species documented in Campbell County, the identification and growing historical record of local flora will support short and long-term recognition of the importance of biodiverse natural communities in central Virginia. This project seeks to (1) document flora diversity in this region, (2) further establish an herbarium teaching and research collection of representative species, and (3) advance our understanding of the flora in relation to natural communities. Identified ecological groups and community types are based upon the Virginia Department of Conservation and Recreation …

Characterizing B1 B-Cells In Zebrafish (Danio Rerio), Alyssa Canelo, Victoria Pacheco

Liberty University Research Week

This project tests the hypothesis that developing antibodies specific to zebrafish CD5 and IgM will enable the reliable identification and characterization of B1 B-cells in zebrafish. B1 B-cells play a critical role in autoimmunity across species, yet their functions remain poorly characterized in zebrafish (Danio rerio). Zebrafish are widely used as a developmental and immunological model organism due to their genetic similarity to humans as well as their transparent embryos. The lack of species-specific antibodies has hindered the study of B1 B-cells in zebrafish.

This project seeks to develop and validate antibodies against zebrafish CD5 and IgM. This will be …

Kinetic Analysis Of Degradation And Maximum Catalytic Capacity For Plant Peroxidase Enzymes, Hayden Stollings

Liberty University Research Week

In 2006, the Environmental Protection Agency (EPA) began investigation into the “forever chemicals” known as PFAS. PFAS, which stand for “per- and polyfluoroalkyl substances,” are a highly stable and toxic compound that were mass produced throughout the 1900’s and was commonly found in common kitchenware, carpets, furniture and even produced in industrial processes.

The function behind PFAS’ stability lies in the abnormally strong Carbon-Fluoride bond, which explains for its initial functionality. Moreso, PFAS can withstand natural degradation in the environment, leading to buildup. Exposure to certain PFAS have been linked to health problems such as increased cholesterol, thyroid issues, reproductive …

Characterizing Novel Peroxidase Activity Through Enzyme Kinetics And Equilibrium Binding, Marika Yelle

Liberty University Research Week

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants, motivating the exploration of peroxidases as potential bioremediation catalysts. This study spectroscopically characterized three peroxidases, with a focus on Pumpkin Skin Peroxidase (PKS). Initial characterization assessed PKS equilibrium binding with various ligands to determine dissociation constants (Kd) for each ligand-enzyme interaction. To characterize PKS catalytic activity, guaiacol oxidation was monitored, with parallel KF trials modelled by Michaelis-Menten kinetics, revealing fluoride-induced increases in Km and Vmax, consistent with an inhibitory effect on substrate binding and catalytic turnover. These findings provide insight into the function of PKS and its potential for environmentally relevant …

Investigating The Role Of The Active-Site Metal In Plant Peroxidase Catalysis, Alexandra Knight

Liberty University Research Week

This study investigates how replacing the native iron heme with manganese or cobalt protoporphyrins affects the enzymatic activity of horseradish (HRP), pumpkin skin (PKS), and butternut squash (BNT) peroxidases. A heme extraction protocol was adapted for generating apoenzymes by removing the native iron heme cofactor, and extraction was confirmed using UV-vis spectroscopy. While initial reconstitution of apo-HRP with manganese protoporphyrin IX appeared to be incomplete based on UV-vis analysis, the results suggested that manganese incorporation modifies the spectroscopic characteristics of the native enzyme. Comparing horseradish, pumpkin skin, and butternut peroxidases will reveal potential differences in metal-dependent activity and stability among …

Morphological Evaluation Of Cadaveric Tissues Integrity Using Histology Staining, Jumana Rizk, Levon Shukrian, Maria Mendoza Lopez, Shanice Rerrie

Liberty University Research Week

The transition from gross anatomical dissection to microscopic evaluation is essential for a comprehensive understanding of human biology. While systemic anatomy reveals the macroscopic organization of the body, histology provides insight into cellular structure and tissue integrity. This study aimed to bridge these disciplines through histological assessment of cadaveric tissues. Our objective was to determine whether tissue samples after prolonged fixation could be processed and stained to meet standards for histology education. Tissue samples of the cerebral cortex, renal cortex, liver lobe, and skeletal muscle were obtained from two cadaveric donors, one male and one female. Tissues were processed, embedded …

“Slaves To Sin:” Harnessing Neuroplasticity To Defeat Habitual Behaviors, Charity Parrott

Liberty University Research Week

How does neuroplasticity affect the life of a Christian? This thesis will seek to address the concepts of habit formation, neuroplasticity, and top-down control through a neurobiological perspective, and how they relate to the Biblical concept of habitual sin. Discoveries in neuroplasticity have revealed the brain to be a malleable organ, capable of rewiring neural connections and, in the hippocampus region, neurogenesis, or the creation of new neurons (Fuchs & Flügge, 2014). This thesis will compile research in neuroplasticity, explore the biological reasons behind the difficulty in escaping patterns of sin, and connect Biblical-based strategies for sin prevention with neurobiology. …

Playing The Past: Girls, Dolls, And Entrepreneurs, Taylor Schmalz

Liberty University Research Week

Mattel, Inc. is a toy company located in California and sells some of the most iconic children’s toys across gender lines. From Hot Wheels, Barbie, Fisher-Price, and over a hundred more toy brands, Mattel, Inc. has touched millions of lives since its creation in 1945. Two of the most famous being American Girl Dolls and the Barbie Doll. Each doll was created by female entrepreneurs and was made to empower little girls in America. While the dolls were created to empower girls, teach feminist ideas, and share women’s history, the dolls face backlash for not being feminist enough, too grown …

Effect Of In-Utero Exposure To A Western-Style Diet On Testis Development, Alexa Ashby, Colton Fitzgerald, Kaylee Vis, Madison Ashby, Marisa Halsey, Nathan Malina, Ryan Anderson

Liberty University Research Week

The increase in western-style dietary patterns has led to an increase in obesity prevalence and its associated comorbidities. Specifically, researchers have found that obesity due to western-style diet (WSD) is negatively correlated with reproductive health in men. This study investigated the effects of inutero exposure to a WSD on testis development, specifically of 12- week-old mice (young adult C57Bl/6J). We hypothesized that in-utero exposure to a WSD would negatively impact testis development and function in male offspring. Using histological hematoxylin and eosin staining and immunohistochemistry method, we examined spermatogenesis in seminiferous tubules, anti-Mullerian hormone (AMH) production by Sertoli cells, and …

How Did Maternal Influenza And Tdap Vaccination Coverage Among Pregnant Women In Mississippi Change From 2018–2021, And How Do Coverage Rates Differ By Race/Ethnicity?, Soala Esther Abere, Atika Ali, Fadzai Nyarugwe, Ifeoluwanimi Shobayo, Jalena Smith

Liberty University Research Week

Maternal vaccination is a critical component of preventive care, yet coverage in the United States remains inconsistent, with persistent racial and age-related disparities. This study examined maternal influenza and Tdap vaccination coverage among pregnant women in Mississippi from 2018–2021, a state with disproportionately high maternal and infant mortality. Using publicly available CDC surveillance data, a quantitative secondary data analysis was conducted to assess vaccination trends and differences by race/ethnicity and maternal age group. After excluding suppressed estimates, 48 observations were analyzed. Overall maternal vaccination coverage averaged 51.55% across all years and demographic groups, with influenza coverage ranging from 49–54% and …

Characterization Of Peroxidase From Butternut Squash Skin: Potential Use In Bioremediation, Ava Witkowski

Liberty University Research Week

Peroxidases are present in many diverse plant species and catalyze chemical oxidations, some that may have significance in biotechnological areas like bioremediation. In several recent papers we characterizes a novel peroxidase from the skin of pumpkin that possessed desirable catalytic activities in relation to a class of environmental contaminants call fluorinated phenolics. In the course of these studies, we were able to do genomic analysis on a related plant species curcubita moscata (butternut squash) and identified a peroxidase with very similar sequence. The current study focusses on the characterization of that novel peroxidase in comparison with the pumpkin skin squash. …

Sem-Edx Analysis Of Animal Hairs For The Presence Of Metals, Sydney Stevens, David Green, George Monroe

Liberty University Research Week

No abstract provided.

Analysis Of The Effects Of Phytocannabinoids Cannabichromene (Cbc), Cannabidiol (Cbd), And Cannabigerol (Cbg) On The Enzyme Faah, Rachel Duncan

Liberty University Research Week

No abstract provided.

Differential Effects Of Curcumin And C646 On Mitogenic And Metabolic Signaling In Insulin-Stimulated Hepg2 Cells, Davis Raz, Rachael De Klerk

Liberty University Research Week

Curcumin and C646 both inhibit p300/CBP histone acetyltransferase (HAT) activity, but they differ markedly in their mechanisms, potency, and effects on cellular function. Both compounds have been investigated as modulators of the insulin-signaling pathway and have shown potential for improving insulin-signaling–related defects in experimental models. Our previous findings showed that curcumin positively impacted a metabolic pathway by reducing IRS-1 phosphorylation at S1101, a negative regulatory site, while maintaining downstream PRAS40 and RPS6 phosphorylation. Although C646 also reduced IRS-1 S1101 phosphorylation, it did not preserve downstream insulin signaling as effectively as curcumin. We hypothesized that target proteins within the mitogenic pathway, …

Evaluating Sexual Dimorphism In An Urban Neonate Regina Septemvittata Population, Arleigh Wagoner, Nathan Wilkins

Liberty University Research Week

Regina septemvittata, commonly known as Queensnakes, are broadly distributed across eastern and central North America. In adult Queensnakes, females average a greater snout-vent length (SVL) than males. Since Queensnakes exhibit size dimorphism and have short life spans and low survival rates, it was hypothesized that neonate Queensnakes begin to display a distinct sex-related difference in SVL within their first few months of life. Our study investigated growth rates of neonate Queensnakes by conducting a capture-mark-recapture (CMR) study over a three-month period (August through October; birth to first brumation) in an urban stream in Lynchburg, VA. Captured snakes were measured (SVL), …

Quantifying Neuropathy With Von Frey Filaments In Mice: Novel Neuropathic Pain Animal Model, Maggie Milhorne

Liberty University Research Week

Neuropathic pain is a chronic pain condition resulting from damage to the nervous system which does not respond to current pharmacological treatments. Previous research in rats has demonstrated that formalin injections can induce chronic pain states and molecular changes within the spinal cord comparable to those of the spinal nerve ligation (SNL), an established surgical model of neuropathic pain. This study aimed to determine whether a subcutaneous injection of 5% formalin is sufficient to induce chronic pain in mice, providing a simple and less invasive model to study chronic pain mechanisms. Mechanical pain sensitivity is assessed using the von Frey …

Not Allowed To Say Goodbye: The German Civilian's Perception Of Aktion T4, 1939 - 1941, Dylan Cumbo

Liberty University Research Week

Despite the public pushback during the sterilization program, little stopped it from continuing. Nazi officials had desired for the program to expand during the early stages of sterilization, their chance coming with the death of a child and the outbreak of World War II. Six centers were designated as the main euthanasia centers along with several other locations used to support it. Gas, lethal injections, and starvations were used to kill the disabled on mass. Despite attempts to keep the program secret, they failed as clues became increasingly apparent. Documented civilian responses were largely antagonistic and actions committed were passive …