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Cxcl16-Dependent Scavenging Of Oxidized Lipids By Islet Macrophages Promotes Differentiation Of Pathogenic Cd8+ T Cells In Diabetic Autoimmunity, Neetu Srivastava, Hao Hu, Orion J Peterson, Anthony N Vomund, Marta Stremska, Mohammad Zaman, Shilpi Giri, Tiandao Li, Cheryl F Lichti, Pavel N Zakharov, Bo Zhang, Nada A Abumrad, Yi-Guang Chen, Kodi S Ravichandran, Emil R Unanue, Xiaoxiao Wan

2020-Current year OA Pubs

The pancreatic islet microenvironment is highly oxidative, rendering β cells vulnerable to autoinflammatory insults. Here, we examined the role of islet resident macrophages in the autoimmune attack that initiates type 1 diabetes. Islet macrophages highly expressed CXCL16, a chemokine and scavenger receptor for oxidized low-density lipoproteins (OxLDLs), regardless of autoimmune predisposition. Deletion of Cxcl16 in nonobese diabetic (NOD) mice suppressed the development of autoimmune diabetes. Mechanistically, Cxcl16 deficiency impaired clearance of OxLDL by islet macrophages, leading to OxLDL accumulation in pancreatic islets and a substantial reduction in intra-islet transitory (Tex

Defining Primary Refractory Large B-Cell Lymphoma, Allison M Bock, Brad S Kahl, Et Al.

2020-Current year OA Pubs

Patients with large B-cell lymphoma (LBCL) that fail to achieve a complete response (CR) or who relapse early after anthracycline-containing immunochemotherapy (IC) have a poor prognosis and are commonly considered to have "primary refractory disease." However, different definitions of primary refractory disease are used in the literature and clinical practice. In this study, we examined variation in the time to relapse used to define refractory status and association with survival outcomes in patients with primary refractory LBCL in a single-center prospective cohort with validation in an independent multicenter cohort. Patients with newly diagnosed LBCL were enrolled in the Molecular Epidemiological …

Displace Study Shows Poor Quality Of Transcranial Doppler Ultrasound For Stroke Risk Screening In Sickle Cell Anemia, Kimberly A Davidow, Robin E Miller, Shannon M Phillips, Alyssa M Schlenz, Martina Mueller, Monica L Hulbert, Lewis L Hsu, Neha Bhasin, Robert J Adams, Julie Kanter

2020-Current year OA Pubs

Children with sickle cell anemia (SCA) are at increased risk of stroke when compared with their age-based counterparts. The Stroke Prevention Trial in Sickle Cell Anemia (STOP) previously demonstrated that with the use of transcranial Doppler ultrasound (TCD; Sickle Stroke Screen) and chronic red cell transfusion, the risk of stroke is reduced by over 90%. The STOP criteria detailed the type and method of measurement required; the time-averaged mean maximum velocity (TAMMV). Unfortunately, it has been difficult to adhere to the appropriate TAMMV measurements. The objectives of this study were to assess the quality of TCD and transcranial Doppler imaging …

Unraveling Facets Of Mecom-Associated Syndrome: Somatic Genetic Rescue, Clonal Hematopoiesis, And Phenotype Expansion, Parvathy Venugopal, Kelly L Bolton, Et Al.

2020-Current year OA Pubs

No abstract provided.

The Cns Relapse In T-Cell Lymphoma Index Predicts Cns Relapse In Patients With T- And Nk-Cell Lymphomas, Rahul S Bhansali, Neha Mehta-Shah, Et Al.

2020-Current year OA Pubs

Little is known about risk factors for central nervous system (CNS) relapse in mature T-cell and natural killer cell neoplasms (MTNKNs). We aimed to describe the clinical epidemiology of CNS relapse in patients with MTNKN and developed the CNS relapse In T-cell lymphoma Index (CITI) to predict patients at the highest risk of CNS relapse. We reviewed data from 135 patients with MTNKN and CNS relapse from 19 North American institutions. After exclusion of leukemic and most cutaneous forms of MTNKNs, patients were pooled with non-CNS relapse control patients from a single institution to create a CNS relapse-enriched training set. …

Attenuating Midline Thalamus Bursting To Mitigate Absence Epilepsy, Ping Dong, Konstantin Bakhurin, Yuhui Li, Mohamad A Mikati, Jianmin Cui, Warren M Grill, Henry H Yin, Huanghe Yang

2020-Current year OA Pubs

Advancing the mechanistic understanding of absence epilepsy is crucial for developing new therapeutics, especially for patients unresponsive to current treatments. Utilizing a recently developed mouse model of absence epilepsy carrying the BK gain-of-function channelopathy D434G, here we report that attenuating the burst firing of midline thalamus (MLT) neurons effectively prevents absence seizures. We found that enhanced BK channel activity in the BK-D434G MLT neurons promotes synchronized bursting during the ictal phase of absence seizures. Modulating MLT neurons through pharmacological reagents, optogenetic stimulation, or deep brain stimulation effectively attenuates burst firing, leading to reduced absence seizure frequency and increased vigilance. Additionally, …

Where Are We Now With Oncolytic Viruses In Melanoma And Nonmelanoma Skin Malignancies?, George Nassief, Angela Anaeme, Karen Moussa, David Chen, George Ansstas

2020-Current year OA Pubs

Skin cancer prognosis has greatly improved recently due to the introduction of immune checkpoint inhibitors (ICIs). However, many patients with advanced skin cancer still experience immunotherapy resistance and disease progression during ICI treatment, thus calling for novel therapeutics which address this treatment gap. Talimogene laherparepvec (T-VEC) has gained popularity in recent years as a viable treatment option for patients with skin cancer. In preclinical studies, T-VEC demonstrated both a direct anti-tumor effect in injected lesions as well as a systemic immune-mediated effect in non-injected lesions, which could pose additional benefits when combined with ICI therapy. Following promising results from the …

Developing The Openflexure Microscope Towards Medical Use: Technical And Social Challenges Of Developing Globally Accessible Hardware For Healthcare, William Wadsworth, Julian Stirling, Catherine Mkindi, Joram Mduda, Valerian L Sanga, Paul T Nyakyi, Thomas Hervé Mboa Nkoudou, Elisée Jafsia, Stephane Fadanka, Kelsey Hummel, Sharmila Anandasabapathy, Richard Bowman

Faculty, Staff and Students Publications

The OpenFlexure Microscope is an accessible, three-dimensional-printed robotic microscope, with sufficient image quality to resolve diagnostic features including parasites and cancerous cells. As access to lab-grade microscopes is a major challenge in global healthcare, the OpenFlexure Microscope has been developed to be manufactured, maintained and used in remote environments, supporting point-of-care diagnosis. The steps taken in transforming the hardware and software from an academic prototype towards an accepted medical device include addressing technical and social challenges, and are key for any innovation targeting improved effectiveness in low-resource healthcare. This article is part of the Theo Murphy meeting issue 'Open, reproducible …

Incidence And Types Of Cardiac Arrhythmias In The Peri-Ictal Period In Patients Having A Generalized Convulsive Seizure, Johnson P Hampson, Shirin Jamal Omidi, Manuela Ochoa-Urrea, Blanca Talavera, Oscar Mancera, Norma J Hupp, Jaison S Hampson, M R Sandhya Rani, Nuria Lacuey, Shiqiang Tao, Rup K Sainju, Daniel Friedman, Maromi Nei, Catherine A Scott, Brian Gehlbach, Stephan U Schuele, Jennifer A Ogren, Ronald M Harper, Beate Diehl, Lisa M Bateman, Orrin Devinsky, George B Richerson, Guo-Qiang Zhang, Samden D Lhatoo

Faculty, Staff and Students Publications

Background and objectives: Generalized convulsive seizures (GCSs) are the main risk factor of sudden unexpected death in epilepsy (SUDEP), which is likely due to peri-ictal cardiorespiratory dysfunction. The incidence of GCS-induced cardiac arrhythmias, their relationship to seizure severity markers, and their role in SUDEP physiopathology are unknown. The aim of this study was to analyze the incidence of seizure-induced cardiac arrhythmias, their association with electroclinical features and seizure severity biomarkers, as well as their specific occurrences in SUDEP cases.

Methods: This is an observational, prospective, multicenter study of patients with epilepsy aged 18 years and older with recorded GCS during …

Utilizing Biosimilars To Help Increase Patients Access And Minimize The Financial Burden Of Health Care System In Egypt, Doaa M. Abd-El-Rady, Iman A. El-Baraky, Samar F. Farid

Bulletin of Faculty of Pharmacy Cairo University

Background: Biologics have provided enhanced clinical advantages, but at expense of enormous expenditures. The exorbitant expense of biologics was somewhat mitigated by the development of biosimilars. But biosimilars market access is still recessed in many countries including Egypt. To achieve biosimilars market sustainability, countries need to evaluate the health economic barriers that biosimilars face in gaining market access.

Methods: A retrospective budget impact analysis was conducted to compare the cost of biosimilars to that of their reference biologics from Egyptian regulatory perspective over a five-year period (2018-2022). Time series forecasting analysis was conducted to predict the cost savings …

Removal Of Textile Dyes By Ecofriendly Aquatic Plants From Wastewater: A Review On Plants Species, Mechanisms, And Perspectives, Manal M. El-Sadaawy, Nancy S. Agib

Blue Economy

Water, an essential resource for sustaining life on our planet, is crucial for both human beings and the overall ecosystem. However, in recent decades, several factors, including population growth, rapid industrialization, urbanization, and unsustainable utilization of natural resources, have significantly impacted the quality of water. Industries such as textiles, leather tanning, cosmetics, and pigmentation extensively employ colored compounds called dyes for various purposes. Synthetic dyes have largely replaced natural dyes due to their superior durability against washing, heat, light, pH, and exposure to biological agents. Azo dyes, sulfur dyes, and pigment dyes are some examples of dyes used in industrial …

Evtol Delivery Drone, Caleb Nass, Brennen Bazaldua, Matthew Mason, Dylan Cummings, Derek Ren

2024 Symposium

The creation of online food ordering and delivery is an invention that has introduced a completely new market with the potential for innovation. Traditional methods of delivery currently focus on individual delivery drivers. But this method depends on variables like traffic and road conditions and uses considerable amounts of fuel while incurring prohibitive costs to employers, which may be impractical for small businesses. To that end, our team’s project seeks to design and fabricate a 3D printed eVTOL delivery drone capable of vertical takeoff and landing along with transition to horizontal flight. We believe that our design will be lightweight, …

Preliminary Analysis Of Per-And Polyfluoroalkyl Substances (Pfas) In Surface Waters Of The West Plains Region Of Spokane County, Jerusha Hampson

2024 Symposium

Per-and Polyfluoroalkyl Substances (PFAS) have become a national issue as regulatory limits for these toxins have just recently been put into the Clean Water Act. The West Plains has been significantly impacted by PFAS contamination due to the decades-long use of aqueous film-forming foam (AFFF) for firefighting training at both Fairchild Airforce Base and the Spokane International Airport. Though research is still underway, it is known that PFAS contamination in West Plains groundwater is significant and widespread. This study focuses on West Plains surface waters, as there is currently less information about how these water bodies are impacted by PFAS. …

Welcome To Cheney App, Timothy Nelson, Nolan Posey, Tanner Stephenson, Daniel Palmer, Matthew Matriciano

2024 Symposium

Welcome to Cheney is a non-profit organization committed to fostering communication, connection, and action within the city of Cheney. Their primary purpose is to provide timely and accurate information to the residents of Cheney. Welcome to Cheney has tried utilizing other forms of social media such as Facebook and Instagram to share information, but their presence is being overshadowed amidst the noise on those platforms. Therefore, the intention of this project is to develop a mobile app with the sole purpose of being a reliable means of sharing important information with the residents of Cheney.

The information being shared on …

Live-Weight Gain Of Steers Grazing African Star Grass Four Herbage Allowances, J Perez-Perez, J O. Hernandez-Velez, J G. Herrera-Haro, R Barcena-Gama

IGC Proceedings (1977-2023)

The objective was to determine weight changes of steers grazing African star grass (Cynodon plectostachyus) at four herbage allowances (2.0, 2.5, 3.0 and 3.5 kg of dry matter/100 kg of live weight day-1). A rotational grazing system with 10 grazing days and 30 days rest was followed. A Split Plot Design with four replications was used. Response variables were: daily gain per steer (DGS) and per hectare (DGH), dry matter forage yield (FY), % of utilization (% U) and in vitro dry matter digestibility (DMIVD). DGS and DGH were not different (P >0.05) among allowances and grazing periods. …

Strategic Use Of Brachiaria Decumbens Pasture To Beef Heifers During Dry Season, E G. Cardoso, N F. Seiffert, J Marques Da Silva, V.P. B. Euclides, J M. Vieira

IGC Proceedings (1977-2023)

The main objective of this study was to evaluate a Brachiaria decumbens pasture, instead of a common rangeland, for zebu beef heifers during the dry season of the year as a means of anticipating the first calving age from four to three years of age, and so improve the production rate of the herd. Daily liveweight gains (DLWG) were analyzed for the effects of year and three stocking rates during the dry season (SR1=1.5; SR2=2.0 and SR3=2.5 head/ha). The main effect on heifers DLWG was the year (P<0.01), but SR was also important (P<0.05), mainly during the last half of the dry season when pastures are under severe constraints such as soil moisture deficit and low temperatures. The lowest SR presented the highest DLWG (0.204a, 0.174ab and 0.145b kg/head, respectively for SR1, SR2 and SR3), during the dry season. If climatic conditions are not exceptionally bad, heifer first calving occurs at 37 months of age when reared on B. decumbens.

Quality Grassland For Dairy Production In North-West Spain, A Gonzalez-Rodriguez

IGC Proceedings (1977-2023)

An experiment with two groups of 15 Friesian cows grazing independent areas, with no N and 240 kg N ha-1, was run during two years to see the levels of milk production based on a grass/white clover pasture, maintaining high levels of herbage quality. N application decrease clover content from 55% to 42%. The total DM response to N was low. Herbage utilization was maintained similar in both treatments. Moderate levels of milk production can be reached, more than 4000 litres cow-1 and 9000 litres ha-1, with low use of concentrates, only 400 kg cow-1. Crude protein levels were high …

Sward Height On The Performance Of Cow-Calf Units And Yearling Steers Grazing Cool Season Pasture, E C. Prigge, W B. Bryan, E L. Nestor

IGC Proceedings (1977-2023)

Performance and grazing time of cow-calf units and yearling steers were determined over four years on naturalized cool season pastures maintained at a sward height of 4-6, 6-8, 8-10, or 10-12 cm. Daily gains of cows and steers responded quadratically (P<0.05) to increasing sward heights, with gains maximized at 8-10 cm. Milk production of the cows and gain of the calves were not affected (P>0.10) by sward height. Grazing days (cow-calf unit/hectare) decreased linearly (P<0.01) with increasing sward height. The proportion of the day spent grazing for cows and steers decreased in a linear manner (P<0.05) as pasture height increased. Cows spent less time grazing than steers (P<0.05) at all but the low pasture height.

Buffer Grazing For A Twelve Month Cow-Calf Production System, W B. Bryan, E C. Prigge, D J. Flaherty, G E. D'Souza

IGC Proceedings (1977-2023)

Set stocking is a widely used grazing management for beef cow-calf production on Appalachian hill land pastures of the USA. A replicated study was conducted to compare a conventional (set stocking) and buffer grazing management. For the buffer system, grazing area was restricted in the spring and hay was harvested from the ungrazed area. In summer, a portion of this area (buffer) was grazed and a second cutting of hay removed from the remaining area. For the conventional treatment a fixed area was set stocked and hay was harvested on an area designated for hay only. Land areas and fertilizer …

Intensive Rotational Grazing Systems For Dairying In A Subtropical Environment: Animal, Plant, And Soil Responses, J H. Fike, C R. Staples, L E. Sollenberger, D A. Graetz

IGC Proceedings (1977-2023)

Forage species, stocking rate, and supplementation rate effects upon performance of lactating dairy cows were studied. Forage mass and nutritive value and nutrient concentration in shallow groundwater also were measured. Cows (n = 44) grazed bermudagrass (Cynodon dactylon) or rhizoma peanut (Arachis glabrata), stocked at 4.9 or 7.4 cows ha-1, and 2.5 or 4.9 cows ha-1, respectively. Pelleted concentrate:whole cottonseed (80:20) was offered twice daily post milking at 1 kg per 2 or 3 kg of daily milk production. Cows grazing rhizoma peanut produced more (P = .076) milk per day but had greater (P = …

High Moisture Corn Supplementation Of Different Dry Matter Content To Cows Grazing Temperate Pasture, D H. Rearte, H Alvarez, F Santini

IGC Proceedings (1977-2023)

The objective of this study was to evaluate grazing dairy cows performance supplemented with high moisture corn of different dry matter content. Cows grazed fresh forage oat day and night and received 5 kg DM high moisture corn daily. Treatments 1 and 2 were high moisture corn of 30% and 18% moisture content respectively. Twenty Holstein cows were used for milk production and composition studies and six rumen fistulated cows were used for rumen digestion studies. Milk production and composition and body weight gain were not affected by the dry matter content of the supplemented high moisture corn. Related to …

Intake Of High Producing Holstein Cows Grazing Pasture Or Fed A Total Mixed Ration, E S. Kolver, L D. Muller

IGC Proceedings (1977-2023)

The ability of a pasture diet to support a high dry matter intake without supplementary feed was determined using 16 high producing Holstein cows. Cows (n=8) were adapted to an all-pasture diet by incrementally reducing the amount of total mixed ration (TMR) fed over four weeks. A control group of cows (n=8) remained in confinement and were fed a TMR. Performance of grazing cows differed significantly (P < 0.001) from TMR fed cows by intake (19.0 vs. 23.4 kg DM/d), milk production (29.6 vs. 44.1 kg/d), milk protein content (2.61 vs. 2.80%), liveweight (562.1 vs. 597.3 kg), and condition score (2.02 vs. 2.50). Results indicate that intake of good quality pasture is higher than previously estimated for high producing Holstein cows in the USA, but that milk production is 15 kg/d less than when cows are fed a TMR.

Cattle Production And Botanical Composition In Continuously Stocked Grass-Clover Swards, A Elgersma, H Schlepers

IGC Proceedings (1977-2023)

The performance of grass-clover mixtures was evaluated under cattle grazing, and the relations between grass variety, clover content and animal production were studied during four years at three locations. The prostrate diploid perennial ryegrass variety formed a dense sward with less clover and less weeds than the tetraploid or the erect diploid ryegrass varieties, which contained about the same amount of clover in the mixture. The ryegrass tiller density was lowest in the tetraploid. These differences between mixtures were not reflected in differences in animal performance or net energy production.

Production Efficiency Benchmarks For Irrigated Dairying In South Eastern Australia, K E. Pritchard, O A. Gyles, D P. Armstrong, S L. Spiteri

IGC Proceedings (1977-2023)

Milk production from grazed irrigated pasture is an important industry in south eastern Australia. Irrigation water is essential for perennial pastures, but the efficiency with which the irrigation water is used to produce milk varies widely between farms. As irrigation water is a limiting resource, future productivity increases will depend on increasing the amount of milk produced per unit of water applied. This research project estimates metabolisable energy utilised from grazed pasture, using energy values for animal requirements, products and feedstuffs. Efficiency criteria, in terms of production per hectare of irrigated pasture, per megalitre of irrigation water, and per cow …

Grazing Management And Nitrogen Fertilizer Effects In Grazing Systems For Beef Cows, D G. Morrison, C C. Willis, D L. Castle, G J. Cuomo

IGC Proceedings (1977-2023)

Four 8-ha areas, primarily Cynodon dactylon forage, were overseeded in autumn with annual ryegrass (Lolium multiflorum) and clover (Trifolium incarnatum and Trifolium repens). Two areas received no nitrogen (N) fertilizer and two received 168 kg N/ha annually. Within N level (2 x 2 factorial), one area was divided into 4 paddocks (pad) and the other into 16 pad for rotational grazing. Spring-calving beef cows were stocked (2.5/ha) year-round on each area with calves weaned in October. Annual dry matter forage accumulation was greater where N was applied (18.2 vs 14.2 Mg/ha, P=.02) but was unaffected by …

Sheep Production On An Annual And Perennial Pasture In Southwest Australia, P Sanford, J Gladman, L Cransberg

IGC Proceedings (1977-2023)

Sheep production on an annual and perennial pasture stocked at 11.5 dse/ha, was compared in a mediterranen environment at Manypeaks in Western Australia. The annual sward comprised of 48% subterranean clover (Trifolium subterraneum L.), 20 % annual grasses (Lolium rigidum Gaudin, Hordeum leporinum Link, Vulpia bromoides (L.) S.F. Gray) and 32% capeweed (Arctotheca calendula L.). The perennial sward was 19% kikuyu (Pennisetum clandestinum), 36% subterranean clover, 43% annual grasses (Lolium rigidum Gaudin, Hordeum leporinum Link, Vulpia bromoides (L.) S.F. Gray) and only 2% capeweed which was displaced by kikuyu. Both pasture types had similar growth …

Milk Production And Composition In Charolais And Polled Hereford Cows On Different Forage Systems, J M. Phillips, R B. Simpson, J D. Shockey, S C. Nickerson

IGC Proceedings (1977-2023)

The objective of this study was to study the effect of different winter and summer forage systems on milk production and composition in beef cows. Charolais and Polled Hereford cows were assigned to receive either grass hay and supplemental feed or ryegrass pasture during the late winter/early spring period, and then allowed to either graze warmseason grass pasture on a continuous or rotational basis during the summer period. Representative cows were machine-milked on day 48 (hay/feed vs ryegrass), on days 117 and 175 (continuous vs rotational grazing) postpartum. Milk weights were recorded and converted to 24- hour yields. Samples were …

Intensive Grazing Management For Dairy Heifers, J R. Rodgers

IGC Proceedings (1977-2023)

After grazing our milking herd on intensive rotational grazing for three years utilizing many paddocks and different types and configurations of fencing to control the wise use of the grass and legume species already on our farm, we moved to develop our second farm into a commercial heifer growing project. This paper will attempt to share our practical experience through three years of grazing upwards of 400 Ayrshire and Holstein heifers from six months of age until just prior to calving. Our ultimate objective is year end financial solvency.

Pasture Production And Weaned Heifer Performance From Tropical Grasses, P Mislevy, F M. Pate, F G. Martin, L M. Rutter

IGC Proceedings (1977-2023)

The purpose of this experiment is to determine the influence of energy supplement (molasses 80% and cottonseed meal 20% slurry) fed to heifers while grazing Florico and Florona stargrass (Cynodon nlemfuensis Vanderyst var nlemfuensis) and Florakirk bermudagrass (C. dactylon) pastures. Stocking rate of heifers was 5.9 animals ha- 1 averaging 230 kg. Heifers were rotated weekly and were on a 4 pasture rotation. Grasses were allowed a 3-wk regrowth period. Mean forage growth rate cycle-1 day-1 was not different (P>0.05) between grasses; however Florico and Florona yielded 8 and 21% more cycle- 1 than Florakirk. Mean herbage …

Seasonal Distribution Of Forage Yield From A "Natural" Pasture Under Rotational Grazing, W R. Kanneganti, T R. Dhiman, R P. Walgenbach, L Massingill, M P. Russelle, L D. Satter

IGC Proceedings (1977-2023)

A 2-yr grazing study was conducted to quantify forage available daily for cattle intake from a natural pasture managed under rotational grazing. Grazing was initiated around 1 May, and was managed with a rotation length of about 17 d each for cycles 1 and 2, and 30 d each for the rest. In 1994, under adequate moisture conditions, forage availability during 5/1-6/1, 6/1-8/15, 8/15-9/15, and 9/15-10/ 15 was 77, 66, 38 and 14 kg DM ha-1 d-1, respectively, resulting in a total yield of 8580 kg ha-1 in 175 d. In 1995, a prolonged period of dry summer reduced the …