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Cgm-Induced Extracellular Trap Formation Augments Inflammation, Joseph Cavataio, Kenneth Wood B.S., Jean Gabriel De Souza Ph.D., Ulrike Klueh Ph.D.

Medical Student Research Symposium

Background and Aims: Subcutaneous continuous glucose monitoring (CGM) devices serve an important role in diabetes management but are prone to biofouling and ensuant device failure. CGM Implantation of CGM devices incites an inflammatory response with initial skin penetration. One specific response is NETosis, in which leukocytes, (e.g. neutrophils), release nuclear DNA to ensnare foreign antigens designated. This study aimed to determine the factors responsible for CGM-induced NETosis.

Methods: In vitro commercial Libre 2 glucose sensors were evaluated for their ability to induce NETosis. These studies utilized flow cytometry and time-lapse imaging, multiplex cytokine panel assessment, and scanning electron …

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