Digital Commons Network^™

Ability Grouping In Primary Physical Education In England: Moving Beyond Binary Discourses And Practices, Shaun D. Wilkinson, Dawn Penney

Research outputs 2022 to 2026

This study recognised that there is currently limited understanding of the extent and nature of ability grouping practices in subject areas other than mathematics and English in primary schools. Using survey methods, this research sought to generate data of sufficient scale to extend understanding of the use of ability grouping practices in primary physical education (PE) in England. Administration of a web-based survey to all (n = 917) state-funded mainstream and special schools catering for students in Key Stage 1 (aged 5–7) and/or Key Stage 2 (aged 7–11) in the North East of England produced a response rate of 27.7% …

Activity Behaviours And Quality Of Life In Patients With Malignant Pleural Effusion, Bianca M. Iacopetta, David C.L. Lam, Jenny C.L. Ngai, Johnny W.M. Chan, Fifian Chiang, Macy M.S. Lui, W. L. Law, Ken K.P. Chan, Joanne Mcveigh, Carolyn J. Peddle-Mcintyre, Y. C.Gary Lee

Research outputs 2022 to 2026

Background and objective Accelerometery is used to measure activity behaviours in patients with malignant pleural effusion (MPE). This study aimed to evaluate physical activity and sedentary behaviour profiles of patients with MPE in Hong Kong, and to explore relationships between activity behaviours and quality of life (QoL). We also compared clinician-recorded performance status measures with objective measures of activity levels. Methods Participants wore an Actigraph GT3X+ accelerometer continuously for 7 consecutive days. The EuroQoL 5 Dimensions 5 Levels (EQ-5D-5L) was used to assess five dimensions of QoL and correlate with activity behaviours. The Eastern Cooperative Oncology Group (ECOG) performance status …

An Updated Social Media Users’ Crisis Response Framework, Violetta Wilk, Francesca Stocco, Saiyidi Mat Roni, Ferry Jie

Research outputs 2022 to 2026

Purpose: This exploratory study aims to investigate social media users’ reactions via user-generated content (UGC) to crisis-affected supply chains, through the lens of Situational Crisis Communication Theory, to: (1) ascertain whether the Social Media Users' Crisis Response (SMUCR) Framework is applicable to a prolonged, multi-brand, global crisis and (2) whether there is any evidence of online brand advocacy (OBA) and online brand detraction (OBD). Design/methodology/approach: Natural language processing (NLP) with Leximancer, enabled thematic and sentiment analyses of 295,024 X (Twitter) posts extracted over a three-year period. Findings: This study found that there were nine stages in social media users’ response …

Associations Between Flavonoid-Rich Food And Flavonoid Intakes And Incident Unhealthy Aging Outcomes In Older United States Males And Females, Nicola P. Bondonno, Yan Lydia Liu, Francine Grodstein, Eric B. Rimm, Aedín Cassidy

Research outputs 2022 to 2026

Background: Our knowledge of the importance of flavonoid-rich foods in preventing unhealthy aging across its different domains is limited. Objectives: This study aimed to examine prospective associations between flavonoid-rich food and flavonoid intakes and indicators of unhealthy aging, namely frailty, impaired physical function, and poor mental health. Methods: We followed up 62,743 females and 23,687 males, all aged ≥60 y, from the Nurses’ Health Study (1990–2014) and Health Professionals Follow-up Study (2006–2018), respectively. Both time-updated and change in intakes of a flavodiet score (an aggregate of intakes of major flavonoid-rich foods and beverages) and individual flavonoid-rich foods and beverages and …

Sleep Discrepancy And Cognitive Function In Community-Dwelling Older Adults, Nadia Soh, Stephanie R. Rainey-Smith, James D. Doecke, Rodrigo Canovas, Romola S. Bucks, Melissa Ree, Michael Weinborn

Research outputs 2022 to 2026

This was the first study to use cluster analysis to characterise sleep discrepancy (the discordance between self-reported and objective sleep) across multiple sleep parameters, in community-dwelling older adults. For sleep efficiency, negative discrepancy (the tendency to self-report worse sleep than objectively-measured) was associated with poorer memory, independent of insomnia severity, depressive symptoms and objective sleep. This suggests a unique role for sleep discrepancy as a possible risk factor for future cognitive decline, and warrants the need for further research.

Complex Tapestry Of Ebooking Determinants: A Moderation Analysis Of Short-Term Rental, E. A. Ndaguba, K. Brown

Research outputs 2022 to 2026

The objective of this study is to investigate the multifaceted determinants influencing booking intentions in the dynamic landscape of short-term rentals. Drawing on established theories in consumer behaviour and hospitality management, the research aims to shed light on the interplay of factors such as price, occupancy rate, reviews, superhost status, instant book feature, listing type, and their potential moderation. By synthesising these insights, the study seeks to provide a deeper understanding of the complex decision-making processes of potential guests in the evolving context of modern hospitality and short-term accommodation platforms.

Submission To The Nsw Standing Committee On Social Issues Inquiry “Impacts Of Harmful Pornography On Mental, Emotional, And Physical Health”, Giselle Woodley, Lelia Green

Research outputs 2022 to 2026

Our submission is formed from a combined experience of decades of working with teens and more recently, the Australian Research Council funded project (DP190102435), Perceptions of harm from adolescents accessing online sexual content, which explored Australian teens and young people’s (aged 11-17) perspectives of Sexually Explicit Material (SEM) including pornography. Qualitative data was collected between 2021 to 2023, and involved 30 teen interviews, with another 19 interviews with teens occurring a year later (49 interviews in total), and four focus groups (n-18) to explore these issues further. 26 parents of these teens from 24 families were also interviewed, followed by …

Cards Against Everyday Sexisms, Mindy Blaise, Emily M. Gray, Jacqueline Ullman, Emma Fishwick, Georgia Beardman

Research outputs 2022 to 2026

This deck of cards has been created using direct quotes from participants who took part in a nation-wide survey about everyday sexisms in Australian Universities. For more information about the project see: www.everydaysexismsau.com

Forecasting Data-Driven System Strength Level For Inverter-Based Resources-Integrated Weak Grid Systems Using Multi-Objective Machine Learning Algorithms, Md Ohirul Qays, Iftekhar Ahmad, Daryoush Habibi, Mohammad A.S. Masoum

Research outputs 2022 to 2026

Shortage of grid-fault level, known as system strength inadequacy, impacts on grid instability and can lead to blackouts. System strength is generally measured by short circuit ratio index at point of coupling (POC) of inverter-based resources (IBRs) and the grid system. Nowadays, accurate knowledge of system strength forecasting for ‘next day’ to ‘next week’ duration is essential to power system operators, owing to the higher-growth of IBRs. However, releavant publications about this subject remain limited when compared with load demand, active and reactive power prediction. Therefore, a data-driven system strength forecasting scheme is presented in this paper to surmount these …

Investigating Falls Risk Awareness In Hospitals Using The Self-Awareness Of Falls Risk Measure (Safrm): Empirical Research Quantitative, Elissa Dabkowski, Simon J. Cooper, Jhodie Duncan, Karen Missen

Research outputs 2022 to 2026

Aim: The overarching aim of this study was to explore patients' falls risk awareness in hospitals using section A of the validated Self Awareness of Falls Risk Measure (SAFRM). Design: Descriptive cross-sectional study design. Setting: Three rural/regional hospitals in the State of Victoria, Australia. Methods: Using a purposive sampling strategy, patients were eligible to participate if aged ≥ 40 years, English-speaking, and have ambulatory capacity prior to hospital admission. Participants were excluded from the study if they returned a Standardised Mini-Mental State Examination (SMMSE) score < 18. Falls risk awareness data was collected from both patient and health professionals using section A of the SAFRM. Patient demographic data was collected from patient medical records. Results: A total of 77 patients (72.9 years ±11.2) and 58 health professionals were recruited. Patients had a significant difference in falls risk awareness when compared to their clinician (z = −2.08, p = 0.038). Regression analyses showed that patients were more likely to overestimate their falls risk if they used anticoagulant medication and if their highest education level was less than or equal year 11. An exploratory factor analysis (EFA) revealed a three-factor solution from section A of the SAFRM, which were labelled Physical Activity Awareness, Cognitive Awareness and Balance Awareness. Conclusions: There was a significant difference in patients' falls risk awareness compared to a health professional. The independent associations of variables with falls risk awareness, such as age, education level and medication use, further our understanding of the differences in falls risk awareness. The findings also establish that the 15-item section A SAFRM is a reliable and feasible falls risk perception measure for use in hospitals, with future research recommended to evaluate the proposed three-factor model with the addition of tailored hospital falls education. Implications for the Profession and/or Patient Care: The findings from this study establish a significant difference in patients' falls risk awareness compared to their health professional. Section A of the SAFRM is a reliable tool for nurses and other health professionals to establish the presence of a falls risk disparity. The ability to empirically measure this disparity and to determine an under- or overestimation of falls risk is a useful addition to clinical practice. The SAFRM facilitates a person-centred approach to falls prevention by providing opportunities for the clinician to collaborate with the patient and tailor fall prevention strategies. Impact: Problem: Inpatient falls in hospital settings. Main Findings: There was a significant difference in patients' falls risk awareness compared to a health professional. Section A of the Self-Awareness of Falls Risk measure is a reliable and feasible tool to identify under- or overestimation of falls risk perception in hospitals. Patients were more likely to overestimate their falls risk if they used anticoagulant medication and if their highest education level was less than year 11. The findings for a three factor-model Physical Activity Awareness, Cognitive Awareness and Balance Awareness could inform future hospital falls education. Impact: Registered nurses, health professionals, inpatients. Reporting Method: STROBE checklist for cross-sectional studies. Patient or Public Contribution: This study involved the collection of data from patient participants and registered nurses.

Towards Equitable Cancer Outcomes For Rural And Remote Communities: Reflections, Lessons And Recommendations, Anna Ugalde, Hannah Jongebloed, Charlene Wright, Helena Rodi, Anna Chapman, Skye Marshall, Drew Aras, Rebecca J. Bergin, Sophie Boffa, Anna Boltong, Michele Conlin, Fiona Crawford–Williams, Carl De Wet, Wasek Faisal, Lan Gao, Harry Gasper, Kate Gunn, Nicolas H. Hart, Theresa Hayes, Florian Honeyball, Elizabeth A. Johnston, Deme Karikios, Nicole Kiss, Lucy Leigh, Patricia M. Livingston, Carmel O'Kane

Research outputs 2022 to 2026

People living in rural and remote areas continue to face significant inequities in cancer outcomes compared to their metropolitan counterparts. Despite advances in cancer control, these disparities persist across the cancer trajectory. This personal view consolidates findings from our Equitable Cancer Outcomes for Rural and Remote Communities series, highlighting survival disadvantages, challenges in measuring and reporting rurality, barriers to implementing evidence-based interventions, and shortcomings in historical policy. We argue for place-based, system-level reform that genuinely partners with rural communities, leverages local strengths, and embeds rural voices in research, policy, and service delivery. Key recommendations include adopting a formal partnership position …

Reimagining Public Art In Thailand : Policy, Practice And Sustainability, Pawinee Boonserm, Nantida Chandransu

Journal of Urban Culture Research

This research examines how governments promote public art through funding, mandate spaces and research support using international case studies. However, challenges such as centralized funding and restrictions on artistic expression persist. The study proposes a framework for "public art" within fine arts, emphasizing interdisciplinary collaboration and sustainable practices in Thailand. It underscores the importance of collaborative creativity, community engagement, and policy advocacy for a thriving public art landscape. Findings reveal the potential of public art to address societal challenges, foster social healing, and contribute to cultural preservation and economic development. Recommendations include promoting decentralized, community-led initiatives, establishing supportive policies, and …

Musical Convergence In Cross-Cultural Jazz Composition : An Analysis Of “Spring Is Back”, Denny Euprasert

Journal of Urban Culture Research

This research analyzes “Spring is Back,” a cross-cultural jazz composition rooted in the traditional Chinese folk song “⼤地回春” (“Earth’s Rebirth in Spring”). Through examining its formal structure, thematic development, and harmonic content, the study sheds light on its artistic significance and global resonance. The composition’s carefully orchestrated structure and thematic transformation blend elements of folk melody with jazz-inspired rhythm and harmony. Utilizing reharmonization techniques, “Spring is Back” enriches its chord progression with extended chords, quartal and quintal harmony, and contrapuntal bass movement, contributing to its vibrant sound. This research illuminates the transformative power of cross-cultural musical convergence in “Spring is …

The Walasuji Tradition : Forgotten Meanings And Values, Fajar Ciptandi, Ulfah Nafi’Ah, Akramullah -

Journal of Urban Culture Research

The Walasuji tradition is one of the cultural activities of the Bugis Makassar community, South Sulawesi, Indonesia. It can be found at wedding celebrations as a large gate made of bamboo called “Baruga” Walasuji. However, it is hard to find Baruga Walasuji at youth’s wedding nowadays. Whereas the tradition has unique meaning and values which are good to preserve. This study employed a data validation triangulation method involving interviews, literature review, and observations. Subsequently, the analysis was carried out using a qualitative approach known as the "Three Layers of Culture" method. The results of the study show how meaning and …

Ancient Service Systems In Historic Houses And Their Contribution To Sustainable Urban Development : The Case Of Architect Sinan’S House, Gülşen Dişli, Gül Gediri-Gökçen

Journal of Urban Culture Research

The service systems of historic buildings, namely, heating, ventilation, cooling, lighting, and drainage play a critical role in the life cycle of buildings and sustainable urban development to preserve traditional knowledge systems. Therefore, a detailed investigation is required before, during, and after any preservation activity to ensure the historic integrity. Hence, this research aims to identify, investigate, and research the original service systems of a historic building, namely, the Architect Sinan's house in Ağırnas, Türkiye, to reveal and sustain these original passive survivability details to future generations and to learn from these details in present constructions. Main methods were site …

The Impact Of War-Induced Trauma On Young Israelis : A Qualitative Examination Of The David’S Circle Project At Koh Phangan, Rachel Lev-Wiesel, Ofra Walter, Yael Rom-Shoshani, Nisara Jaroenkajornkij, Bussakorn Binson

Journal of Urban Culture Research

This qualitative study explores the psychological impact of war-induced trauma on young Israelis who sought refuge and healing in Koh Phangan, Thailand, through participation in the David’s Circle Project. The project provides a supportive, non-clinical space for the October 7th trauma Nova survivors and young reserve soldiers. In-depth, semi-structured interviews and group storytelling revealed common themes of emotional dislocation, mistrust, and existential uncertainty. The findings underscore the importance of alternative, culturally sensitive healing environments for trauma recovery and point to the therapeutic value of grouping in a safe physical and emotional space. Implications for trauma-informed care and post-conflict rehabilitation for …

Creative Thai Art Learning For Urban Society’S Well-Being, Apichart Pholprasert, Soamshine Boonyananta, Intira Phrompan, Suchart Imsamraan, Wanalee Chanrangsri, Priyasri Promchinda

Journal of Urban Culture Research

Lifestyles in crowded, fast-paced urban societies have created conditions that negatively affect physical, mental, and social well-being. This research aimed to develop and implement creative Thai art learning activities to promote well-being in urban communities. A mixed-methods approach was employed in four stages: (1) documentary research and community surveys, including interviews with 5 community leaders and 100 residents in the Rama IV area; (2) development of creative Thai art learning kits; (3) implementation of learning activities with a sample of 500 community residents; and (4) data collection involved satisfaction and well-being assessments and follow-up interviews; the resulting data were then …

Lakhon Rong Songs Of The Nakhon Bantoeng Troupe (Mae Bunnak Kanlayanamit) & The Phenomenon Of Music In Siam, Tharanat Hin-On, Poonpit Amatayakul, Narongchai Pidokrajt, Anak Charanyananda, Nachaya Natchanawaku

Journal of Urban Culture Research

This study examines the evolution of lakhon rong, a form of Thai musical theater, within the context of Siam’s socio-political and cultural changes during the early 20th century, particularly under the influence of Western modernization. The research focuses on the Nakhon Bantoeng Troupe, exploring how adaptations in lakhon rong performances reflected and contributed to the broader societal efforts towards crafting a modern Thai identity. The integration of Western musical elements with traditional Thai forms exemplifies strategic cultural negotiation, aligning with theories of adaptation and appropriation. Through an analysis of play scripts and historic recordings, this study highlights how cultural practices …

Towards A Sustainable Play-Ground : Performance Ecosystems For A Livable Bangkok Neighborhood, Pornrat Damrhung

Journal of Urban Culture Research

This article examines an effort to turn urban research into creative placemaking by using performances to make a livable Bangkok neighborhood more vibrant. Research explored diverse stakeholders of Samyan-Banthat Thong in central Bangkok during COVID-19. This exploration found opportunities to link the neighborhood’s entrepreneurs, students, visitors and property managers, and discovered key characteristics of the complex and changing neighborhood. We used this knowledge to design a small festival bringing performing artists into the area’s main park and to important sites surrounding it for a weekend of live performances, workshops and demonstrations. By using performance to attract diverse publics to the …

Journal Policies, Jucr Staff

Journal of Urban Culture Research

No abstract provided.

Interpreting Literary Heritage : “Lilit Phra Law” Through Dance As Part Of Heritage Interpretation For The 21st Century, Naraphong Charassri

Journal of Urban Culture Research

This article explores how literary heritage can be reinterpreted through creative dance to sustain its relevance in the 21st century. It derives from a dissertation-length study entitled “Interpreting Literary Heritage: Lilit Phra Law through Dance as Part of Heritage Interpretation for the 21st Century.” The research employed six methods: literature review, personal experiential analysis, field studies, media exploration, symposium discussions, and structured interviews. Data collection was conducted in Thailand from March 2004 to April 2024, involving performing arts professionals, music and drama academics, senior practitioners from the Office of Performing Arts, the Fine Arts Department, Ministry of Culture, and both …

Concept-Based Curriculum: An Evidence-Based Approach To Developing A New Framework For Dietetics Education?, Andrea Begley, Janica Jamieson, Gemma Jenkins, Janeane Dart, Lauren T. Williams, Simone Gibson, Fiona E. Pelly, Hattie H. Wright, Claire Palermo

Research outputs 2022 to 2026

Aims: Concept-based approaches to curricula have been proposed as a solution to this issue, and 56 concepts have been proposed for dietetics curricula. This study aimed to develop a framework for a dietetic concept-based curriculum. Methods: A pragmatic approach was employed by a team of nine experienced Australian dietetic educators. Iterative processes of idea generation, reflection, group discussion, and consensus formation were applied by seven of the academics in a two-day face-to-face workshop and four online meetings to further develop the 56 curriculum concepts previously identified for dietetics by Tweedie et al. The discussions were supported by document analysis and …

Effect Of Exercise On Bone-Related Outcomes In Patients With Cancer Affected By Bone Metastases Or Bone Loss: A Systematic Review And Meta-Analysis, Anita Borsati, Gloria Adamoli, Diana Giannarelli, Lorenzo Belluomini, Andrea Trevisan, Piero Schenal, Francesco Bettariga, Anna M. Markarian, Federico Schena, Michele Milella, Robert U. Newton, Sara Pilotto, Alice Avancini

Research outputs 2022 to 2026

Introduction: This systematic review and meta-analysis aimed to evaluate the effects of exercise interventions on bone mineral density (BMD), bone mineral content (BMC), and bone turnover markers in patients with cancer and compromised bone health. Materials and Methods: A comprehensive search of four electronic databases was conducted to identify randomized controlled trials evaluating the effect of exercise intervention compared to supplementation or bone-targeted agents only on BMD, BMC and bone turnover markers. Meta-analyses were performed using random-effects models to calculate effect sizes (ES) with 95% confidence intervals (CI). Results: Eighteen studies were included, involving 1,478 patients with mainly breast and …

Representing Climate Wreckers, Camila Bustos

Elisabeth Haub School of Law Faculty Publications

In recent years, lawyers have become increasingly aware of the implications of the climate crisis for legal practice. Amidst this context, United Nations Secretary General Ant6nio Guterres has urged recent graduates to decline work on behalf of "climate wreckers." This Article examines how professional responsibility rules and principles in the United States should be interpreted on a warming planet, particularly in the context of attorneys representing so-called "climate wreckers" in civil matters. I use the term "climate wrecker" to refer to fossil fuel corporations and trade associations that have engaged in public disinformation campaigns to stall climate action and sow …

Dynamics Of Water Mass Exchange Across The Central Ross Sea Slope, Yuanjie Chen, Zhaoru Zhang, Pasquale Castagno, Xianxian Han, Michael S. Dinniman, Zhiqiang Liu, Chuning Wang

OES Faculty Publications

The central Ross Sea slope (CRSS) is a critical region for water mass exchange, significantly influencing the physical and biological processes of the Ross Sea shelf and the global overturning circulation. This study utilizes a high-resolution ocean-ice shelf-sea ice coupled model to investigate the mechanisms driving this exchange, involving the onshore transport of circumpolar deep water (CDW) and the offshore transport of dense shelf water (DSW). Combining numerical simulations and mooring observations, this work reveals high-frequency oscillations with a periodicity of ∼32 hr in CDW transport induced by Topographic Rossby Waves and DSW transport induced by cyclonic eddies. These findings …

Dynamic Stability Test Technique For Blunt Bodies In A Magnetic Suspension And Balance System, Caleb Hull, Colin Britcher, Dave E. Cox, Mark Schoenenberger, Hisham M. Shehata

Mechanical & Aerospace Engineering Faculty Publications

Techniques for the measurement of subsonic dynamic stability derivatives of blunt-body re-entry capsules are being developed using a Magnetic Suspension and Balance System at NASA Langley Research Center. The measured aerodynamics of a 45-degree sphere-cone similar to configurations being considered for Mars Sample Return Earth Entry Vehicle are reported. A novel test method has been developed where forced oscillatory translation of a test article is used to excite yaw attitude oscillations. The forced motion is then halted to observe free-to-oscillate behavior of the test article. Parameter identification methods are used to extract static and dynamic yawing moment stability characteristics from …

Fault Tree Analysis For Robust Design, Jonathan Degroff, Gene Jean-Win Hou

Mechanical & Aerospace Engineering Faculty Publications

The objective of this research is to incorporate system failure into a robust design formation and solution process. The system failure referred to here will be built using fault tree analysis (FTA), which will take all lower-level failure events into consideration. Two examples are investigated here. One will directly treat the probabilities of the basis events as design variables, The other will be formulated in five different models: deterministic design optimization, the reliability index-based, the “and” gate-based, the “or” gate-based and the “inhibit” gate-based robust design. Their corresponding optimization solutions will be compared with each other. The post-optimality analysis of …

Polarized Positrons At Ce⁺Baf, A. Ushakov, J. Benesch, M. Bruker, L. Cardman, J. Conway, S. Covrig Dusa, P. Degtiarenko, Y. Enomoto, S. Gessner, P. Ghoshal, S. Gopinath, J. Grames, C. Gulliford, S. Habet, G. Hays, C. Hernández-García, A. Hofler, R. Kazimi, M. Kostin, V. O. Kostroun, F. Lin, V. Lizárraga-Rubio, K. Mahler, Y. Morikawa, S. Nagaitsev, S. Ogur, G. Palacios-Serrano, N. Raut, B. Rimmer, Y. Roblin, A. Seryi, K. Smolenski, M. Stutzman, R. Suleiman, A. Sy, N. Taylor, D. Turner, A. Ushakov, C. Valerio-Lizarraga, E. Voutier, S. Wang, Y. Zhang

Mechanical & Aerospace Engineering Faculty Publications

A baseline concept for a continuous wave (CW) polarized positron injector was developed for the Continuous Electron Beam Accelerator Facility (CEBAF) at Jefferson Lab [1]. This concept is based on positron beam generation by a high current polarized electron beam (1 mA, 120 MeV, >85% polarization) irradiating a rotating water-cooled tungsten target or liquid metal target. The update on the development of the Ce⁺BAF injector concept including the polarized electron source, the development of high power targets, simulations of positron capture, the design of the transport line from the positron injector to the CEBAF North Linac and the planned experiment …

Revealing Spatiotemporal Neural Activation Patterns In Electrocorticography Recordings Of Human Speech Production By Mutual Information, Julio Kovacs, Dean Krusienski, Minu Maninder, Willy Wriggers

Mechanical & Aerospace Engineering Faculty Publications

Background

Spatiotemporal mapping of neural activity during continuous speech production has been traditionally approached using correlation coefficient (CC) analysis between cortical signals and speech recordings. A prior study employed this approach using electrocorticography (ECoG) data from participants who underwent invasive intracranial monitoring for epilepsy. However, CC cannot detect nonlinear relationships and is dominated by the correspondence between periods of silence and of non-silence.

New Method

We introduce the mutual information (MI) measure, which can capture both linear and nonlinear dependencies. We validated CC and MI on the sub-second spatiotemporal brain activity recorded during continuous speech tasks. To refine the results, …

Traction Force Microscopy Using An Epifluorescence Microscope: Experimental Considerations And Caveats, Zaria Booth, Mazen Mezher, Rudra Patel, Venkat Maruthamuthu

Mechanical & Aerospace Engineering Faculty Publications

Forces exerted by cells due to their internal contractility play fundamental roles in a host of processes, including adhesion, migration, survival and differentiation. Traction force microscopy (TFM) enables the determination of forces exerted by cells or cell collectives on their environment, which is typically taken to be an extra-cellular matrix (ECM)-coated substrate. Sample preparation for TFM involves the plating of cells onto an environment embedded with fiducial markers. The imaging of these fiducial markers in the presence and absence of the cells then enables calculation of the displacement of localized regions of the environment, and, consequently, the spatial distribution of …